The present study was focused on the biodegradation of pine needles by the production of hydrolyt... more The present study was focused on the biodegradation of pine needles by the production of hydrolytic enzymes viz. cellulase, xylanase, laccase as well as reducing sugars production w.r.t optimization of moisture (Basal salt media) ratio for the newly isolated white rot fungi Phanerochaete sp. S7, Armillaria sp. K3 and Phanerochaete chrysosporium [NCIM 1197] were optimized under solid state fermentation and maximum of cellulase (52.27 U/g) with specific activity of 2.10 was observed in P. chrysosporium using untreated pine needles as substrate at 1:4 of moisture ratio, while maximum of xylanase and laccase were expressed by white rot Armillaria sp K3 at 1:4 of moistening agent i.e.174.00 U/g and 5.94 U/g with respect to their specific activities of 7.65 and 0.28 using untreated pine needles. In case of reducing sugars maximum of 15.48 mg/g was released at 1:4 of moisture using pretreated pine needles which is followed by the value of 14.88 mg/g at 1:3 by P. chrysosporium in untreated pine needles. In our study the newly isolated white rot fungi Phanerochaete sp. S7 and Armillaria sp. K3 were found to be potential strains for the hyper production of lignin degrading enzyme i.e. laccase as compared to the standard strain Phanerochaete chrysosporium [NCIM 1197].
The Journal of Maternal-Fetal & Neonatal Medicine, 2020
Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp a... more Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp and Mycoplasmahominis in amniotic fluid of patients at high risk for intra-amniotic infection,
Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp a... more Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp and Mycoplasmahominis in amniotic fluid of patients at high risk for intra-amniotic infection,
... MARIA ELENA NILO*, JULIAN ALVARADO*, MARCELO RAMIREZ y EDUARDO ESPEJO*. ... Se analizan muest... more ... MARIA ELENA NILO*, JULIAN ALVARADO*, MARCELO RAMIREZ y EDUARDO ESPEJO*. ... Se analizan muestras de sangre periférica del recién nacido y de la madre mediante la técnica de Strout, la cual consiste en la centrifugación de 10 capilares de sangre, de los cuales se ...
The catabolism of dimethoxybenzil, anisoin and hydroanisoin in nitrogen-limited stationary cultur... more The catabolism of dimethoxybenzil, anisoin and hydroanisoin in nitrogen-limited stationary cultures of the brown-rot fungi Wolfiporia cocos and Gloeophyllum trabeum was analyzed. These three 1, 2-diarylethane lignin model compounds, which differ in the degree of ...
The effects of selected culture parameters on the solid-state fermentation of radiata pine (Pinus... more The effects of selected culture parameters on the solid-state fermentation of radiata pine (Pinus radiata) by the brown-rot fungus Gloeophyllum trabeum were examined. Oxygen concentration is critical for wood decay; a twofold increase was observed when 21% 02 was employed, as compared to 5% 02. The highest yields were obtained with p02 > 50%. Although optimum pH for fungal growth was 4.5, pH lower than 4.0 appeared to be essential for wood degradation by these fungi. Culture conditions allowing the highest decay capacities were then employed to follow the chemical changes occurring during decay of radiata pine by G. trabeum, Lentinus lepideus, and Wolfiporia cocos and their effects on rumen digestibility were assessed. Dry matter digestibility increased from 0 to more than 20% during the first week of wood colonization by the three tested fungi. Then, after 4 weeks of incubation, digestibility feU to less than 5%for W. cocos and L. lepideus. Increase in digestibility could be related to polysaccharide depolymerization, as well as to the alkali-solubilizable fraction of structural polysaccharides and lignin in decayed wood.
The present study was focused on the biodegradation of pine needles by the production of hydrolyt... more The present study was focused on the biodegradation of pine needles by the production of hydrolytic enzymes viz. cellulase, xylanase, laccase as well as reducing sugars production w.r.t optimization of moisture (Basal salt media) ratio for the newly isolated white rot fungi Phanerochaete sp. S7, Armillaria sp. K3 and Phanerochaete chrysosporium [NCIM 1197] were optimized under solid state fermentation and maximum of cellulase (52.27 U/g) with specific activity of 2.10 was observed in P. chrysosporium using untreated pine needles as substrate at 1:4 of moisture ratio, while maximum of xylanase and laccase were expressed by white rot Armillaria sp K3 at 1:4 of moistening agent i.e.174.00 U/g and 5.94 U/g with respect to their specific activities of 7.65 and 0.28 using untreated pine needles. In case of reducing sugars maximum of 15.48 mg/g was released at 1:4 of moisture using pretreated pine needles which is followed by the value of 14.88 mg/g at 1:3 by P. chrysosporium in untreated pine needles. In our study the newly isolated white rot fungi Phanerochaete sp. S7 and Armillaria sp. K3 were found to be potential strains for the hyper production of lignin degrading enzyme i.e. laccase as compared to the standard strain Phanerochaete chrysosporium [NCIM 1197].
The Journal of Maternal-Fetal & Neonatal Medicine, 2020
Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp a... more Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp and Mycoplasmahominis in amniotic fluid of patients at high risk for intra-amniotic infection,
Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp a... more Escobar (2020) Comparison of two identification and susceptibility test kits for Ureaplasma spp and Mycoplasmahominis in amniotic fluid of patients at high risk for intra-amniotic infection,
... MARIA ELENA NILO*, JULIAN ALVARADO*, MARCELO RAMIREZ y EDUARDO ESPEJO*. ... Se analizan muest... more ... MARIA ELENA NILO*, JULIAN ALVARADO*, MARCELO RAMIREZ y EDUARDO ESPEJO*. ... Se analizan muestras de sangre periférica del recién nacido y de la madre mediante la técnica de Strout, la cual consiste en la centrifugación de 10 capilares de sangre, de los cuales se ...
The catabolism of dimethoxybenzil, anisoin and hydroanisoin in nitrogen-limited stationary cultur... more The catabolism of dimethoxybenzil, anisoin and hydroanisoin in nitrogen-limited stationary cultures of the brown-rot fungi Wolfiporia cocos and Gloeophyllum trabeum was analyzed. These three 1, 2-diarylethane lignin model compounds, which differ in the degree of ...
The effects of selected culture parameters on the solid-state fermentation of radiata pine (Pinus... more The effects of selected culture parameters on the solid-state fermentation of radiata pine (Pinus radiata) by the brown-rot fungus Gloeophyllum trabeum were examined. Oxygen concentration is critical for wood decay; a twofold increase was observed when 21% 02 was employed, as compared to 5% 02. The highest yields were obtained with p02 > 50%. Although optimum pH for fungal growth was 4.5, pH lower than 4.0 appeared to be essential for wood degradation by these fungi. Culture conditions allowing the highest decay capacities were then employed to follow the chemical changes occurring during decay of radiata pine by G. trabeum, Lentinus lepideus, and Wolfiporia cocos and their effects on rumen digestibility were assessed. Dry matter digestibility increased from 0 to more than 20% during the first week of wood colonization by the three tested fungi. Then, after 4 weeks of incubation, digestibility feU to less than 5%for W. cocos and L. lepideus. Increase in digestibility could be related to polysaccharide depolymerization, as well as to the alkali-solubilizable fraction of structural polysaccharides and lignin in decayed wood.
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Papers by Eduardo Espejo