PKCε is a transforming oncogene and a predictive biomarker of various human cancers. However, a p... more PKCε is a transforming oncogene and a predictive biomarker of various human cancers. However, a precise in vivo link of PKCε to cancer induction, progression and metastasis remain undefined. To achieve these goals, we generated tissue specific conditional PKCε knockout mice (PKCε-CKO) using cre-lox technology. Homozygous PKCεLoxP/LoxP mice have normal body weight and phenotype. To determine what effect loss of PKCε would have on the prostate, the PKCεLoxP/LoxP mice were bred to probasin cre (PB-Cre4+) mice which express cre specifically in the prostate epithelium of postnatal mice. Western blot and immunohistochemical analyses showed reduced levels of PKCε specifically in the prostate of PKCε-CKO mice. Histopathological analyses of prostate from both PKCεLoxP/LoxP and prostate PKCε-CKO mice showed normal pathology. To determine the functional impact of prostate specific deletion of PKCε on prostate tumor growth, we performed an orthotopic xenograft study. Transgenic adenocarcinoma o...
Insulin/IGF-1-like signalling (IIS) and dietary restriction (DR) are the two major modulatory pat... more Insulin/IGF-1-like signalling (IIS) and dietary restriction (DR) are the two major modulatory pathways controlling longevity across species. Here, we show that both pathways license a common chromatin modifier, ZFP-1/AF10. The downstream transcription factors of the IIS and select DR pathways, DAF-16/FOXO or PHA-4/FOXA, respectively, both transcriptionally regulate the expression of zfp-1. ZFP-1, in turn, negatively regulates the expression of DAF-16/FOXO and PHA-4/FOXA target genes, apparently forming feed-forward loops that control the amplitude as well as the duration of gene expression. We show that ZFP-1 mediates this regulation by negatively influencing the recruitment of DAF-16/FOXO and PHA-4/FOXA to their target promoters. Consequently, zfp-1 is required for the enhanced longevity observed during DR and on knockdown of IIS. Our data reveal how two distinct sensor pathways control an overlapping set of genes, using different downstream transcription factors, integrating poten...
Chronic exposure to ultraviolet radiation (UVR) is linked to the development of cutaneous squamou... more Chronic exposure to ultraviolet radiation (UVR) is linked to the development of cutaneous squamous cell carcinoma (SCC), a non-melanoma form of skin cancer that can metastasize. Tumor necrosis factor-alpha (TNFα), a pro-inflammatory cytokine, is linked to UVR-induced development of SCC. To find clues about the mechanisms by which TNFα may promote UVR-induced development of SCC, we investigated changes in the expression profiling of microRNAs (miRNA), a novel class of short noncoding RNAs, which affects translation and stability of mRNAs. In this experiment, TNFα knockout (TNFα KO) mice and their wild type (WT) littermates were exposed to acute UVR (2.0 kJ/m2) and the expression profiling of epidermal miRNA was determined 4hr post UVR exposure. TNFα deletion in untreated WT mice resulted in differential expression (log fold change>1) of seventeen miRNA. UVR exposure in WT mice induced differential expression of 22 miRNA. However, UVR exposure in TNFα KO mice altered only two miRNA...
APA PsycNET Our Apologies! - The following features are not available with your current Browser c... more APA PsycNET Our Apologies! - The following features are not available with your current Browser configuration. - display, print, save, export, and email selected records - get My List count - save record to My List - get references ...
Protein kinase C epsilon (PKCε), a Ca,(2+)-independent phospholipid-dependent serine/threonine ki... more Protein kinase C epsilon (PKCε), a Ca,(2+)-independent phospholipid-dependent serine/threonine kinase, is among the six PKC isoforms (α,δ, ε, η, μ, ζ) expressed in both mouse and human skin. Epidermal PKCε level dictates the susceptibility of PKCε transgenic (TG) mice to the development of cutaneous squamous cell carcinomas (SCC) elicited either by repeated exposure to ultraviolet radiation (UVR) or by using the DMBA initiation-TPA tumor promotion protocol (Cancer Res, 2004, 64:7756-65). Histologically, SCC in TG mice, like human SCC, is poorly differentiated and metastatic. Our earlier studies to elucidate mechanisms of PKCε-mediated development of SCC, using either DMBA-TPA or UVR, indicated elevated release of cytokine TNFα. To determine whether TNFα is essential for the development of SCC in TG mice, we generated PKCε transgenic mice//TNFα-knockout (TG//TNFαKO) by crossbreeding TNFαKO with TG mice. We now present that deletion of TNFα in TG mice inhibited the development of SCC ...
Lowering insulin-IGF-1-like signalling (IIS) activates FOXO transcription factors (TF) to extend ... more Lowering insulin-IGF-1-like signalling (IIS) activates FOXO transcription factors (TF) to extend life span across species. To study the dynamics of FOXO chromatin occupancy under this condition in C. elegans, we report the first recruitment profile of endogenous DAF-16 and show that the response is conserved. DAF-16 predominantly acts as a transcriptional activator and binding within the 0.5 kb promoter-proximal region results in maximum induction of downstream targets that code for proteins involved in detoxification and longevity. Interestingly, genes that are activated under low IIS already have higher DAF-16 recruited to their promoters in WT. DAF-16 binds to variants of the FOXO consensus sequence in the promoter proximal regions of genes that are exclusively targeted during low IIS. We also define a set of 'core' direct targets, after comparing multiple studies, which tend to co-express and contribute robustly towards IIS-associated phenotypes. Additionally, we show th...
. It is commonly known as Jin Gang Teng in Chinese, Chobchini in Hindi, Madhusnuhi in Sanskrit an... more . It is commonly known as Jin Gang Teng in Chinese, Chobchini in Hindi, Madhusnuhi in Sanskrit and china root in English. It possesses antiinflammatory, diuretic, anti-diabetic, anti-psoriatic, digestive properties. There is no authentic protocol for the pharmacognostic parameters and anti-diabetic activity of Smilax china L. So, in the present study a detailed investigation of its pharmacognostic properties and anti-diabetic activity was performed. The pharmacognostic study included powder microscopy, loss of weight on drying, ash values, swelling index, and fluorescence activity. In addition, extracts were obtained using petroleum ether, chloroform, alcohol, hydroalcoholic, and aqueous solvents and the phytochemical constituents present in these extracts were subjected to a variety of chemical tests. Among these extracts, the hydroalcoholic and aqueous fractions exhibited anti-diabetic activity in rats with alloxan-induced diabetes.
Journal of experimental therapeutics & oncology, 2015
Etiopathology of tumor biology is highly complex and ovarian cancer is one of the important gynae... more Etiopathology of tumor biology is highly complex and ovarian cancer is one of the important gynaecological neoplasia associated with high risk of mortality rate. Methlenetetrahydrofolate reductase (MTHFR) mutation are commonly linked to folate metabolism with increased risk factor for the development of neural tube defects, recurrent pregnancy loss and development of several type of cancer but genetic interaction between two alleles of MTHFR has been poorly defined in ovarian cancer in India. Hence, present study becomes imperative with the aim to assess the alleles frequency of MTHFR (C677T & A1298C) gene polymorphism using PCR based RFLP analysis. The O.R at 95% confidence interval (C.I.) was computed between cases and their respective controls to determine "risk factor". Interestingly, our findings reveals highly significant (p < 0.001) difference in heterozygous (CT) condition of C677T allele by computing odd ratio (0.12 at 95% C.I, 0.021-0.0428; P for trend = 0.001...
We present here that heat-shock protein 90 (Hsp90) inhibitor 17-(allylamino)-17-demethoxygeldanam... more We present here that heat-shock protein 90 (Hsp90) inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17AAG), when topically applied to mouse skin, inhibits UVR-induced development of cutaneous squamous cell carcinoma (SCC). In these experiments, DMSO:acetone (1:40 v/v) solution of 17AAG (500 nmol) was applied topically to mouse skin in conjunction with each UVR exposure (1.8 kJ m À 2 ). The UVR source was Kodacelfiltered FS-40 sun lamps (approximately 60% UVB and 40% UVA). In independent experiments with three separate mouse lines (SKH-1 hairless mice, wild-type FVB, and protein kinase C epsilon (PKCe)-overexpressing transgenic FVB mice), 17AAG treatment increased the latency and decreased both the incidence and multiplicity of UVRinduced SCC. Topical 17AAG alone or in conjunction with UVR treatments elicited neither skin nor systemic toxicity. 17AAG-caused inhibition of SCC induction was accompanied by a decrease in UVR-induced (1) hyperplasia, (2) Hsp90b-PKCe interaction, and (3) expression levels of Hsp90b, Stat3, pStat3Ser727, pStat3Tyr705, pAktSer473, and matrix metalloproteinase (MMP). The results presented here indicate that topical Hsp90 inhibitor 17AAG is effective in prevention of UVR-induced epidermal hyperplasia and SCC. One may conclude from the preclinical data presented here that topical 17AAG may be useful for prevention of UVR-induced inflammation and cutaneous SCC either developed in UVR-exposed or organ transplant population.
Recent patents on drug delivery & formulation, Jan 29, 2015
Fast Dissolving/Disintegrating Dosage Forms (FDDFs) are formulated and developed for immediate dr... more Fast Dissolving/Disintegrating Dosage Forms (FDDFs) are formulated and developed for immediate drug delivery, quicker onset of action, and/or as age appropriate dosage forms meant for special populations like paediatrics, , psychotic, dysphagic, and frequent traveller patients. FDDFs family include tablets (FDTs), oral films (FDOF), mini tablets, capsules, pellets, granules etc. FDDFs dissolve/disintegrate quickly in saliva resulting in solution/suspension for oral ingestion and thereby eliminate the need for water for swallowing the dosage form.FDTs market, in regions of US, Europe, and Japan, grew more than $8.4 billion in 2011 and is expected to reach to $12 billion globally by 2018[1]. FDOF market is expected to attain $1 billion mark in 2015 [1]. FDDFs have successfully entered in Pharmacopoeia as Orally Disintegrating Tablets (US and Japan) or Orodispersible Tablets (ODT or OD Tablet) [Europe and Japan] and orodispersible films (ODF) [Europe].FDDFs have recently shifted their ...
Proper disposal and/or recycling of different industrial waste materials have long been recognize... more Proper disposal and/or recycling of different industrial waste materials have long been recognized as a prime environmental concern throughout the world, and fly ash is major amongst them. In the present study, we tried to assess the feasibilities of possible effective and safe utilization of fly ash as soil amendment in Indian paddy field and its impact on rice plants, especially at growth and yield level. Our results showed that certain doses of fly ash amendments have significantly improved the physico-chemical and mineralogical properties of paddy field soil, and at lower level of amendments, fly ash induced the growth performances of three rice cultivars too. Grain yield and grain quality also responded similarly as per the growth responses. However, differential cultivar response was observed accordingly, and cultivar Sugandha-3 showed higher yield as compared with cultivars Sambha and Saryu-52. Based on the observed results, it was concluded that up to a certain level, fly ash amendments could be beneficial for Indian paddy field and can be utilized as feasible management strategy for the disposal of this major industrial waste.
Journal of clinical and diagnostic research : JCDR, 2014
Pre-Eclampsia (PE) affects 6-31% of pregnant women with multiple gestations. There are conflictin... more Pre-Eclampsia (PE) affects 6-31% of pregnant women with multiple gestations. There are conflicting reports on the association of PE with Chorionicity and zygosity; however, there is a lack of information on this potential association in a population of pregnant Asian Indian women. To determine as to whether chorionicity and zygosity were associated with PE in a population of Asian Indian women with twin gestations. A retrospective observational study was done at a single tertiary care centre in Southern India. The study included pregnant women with twin gestations, who was delivered at the study institute in 2012. Hypertension in pregnancy was categorized, based on the criteria of the International Society for the Study of Hypertension in Pregnancy. Chorionicity was determined by using ultrasonography and zygosity was determined, based on clinical criteria. Point estimates and the 95% Confidence Intervals (CI) around point estimates of PE and associations of chorionicity and zygosit...
Field experiments were conducted to evaluate the effect of fly ash on growth and yield of three l... more Field experiments were conducted to evaluate the effect of fly ash on growth and yield of three locally grown cultivars of an important leguminous plant mung bean (Vigna radiata L.) on soil amended with different concentrations of fly ash. The values of pH, EC, WHC, soil cations, total heavy metals of the soil increased; however, values of BD, NH(4)(+)-N, NO(3)(-)-N, total N, organic carbon (OC), organic matter (OM), available P and cation exchange capacity (CEC) decreased with fly ash incorporation in the soil. Fly ash amendment led to improve the growth performance and various yield attributes. An increase in all the growth parameters was recorded in 10% fly ash amended (FAA) soil for cv M. Jyoti and M. Janpriya, while 5% FAA soil was most suitable for cv M. Jagriti. Yield (gm(-2)) also increased significantly by 40.6% and 33.9% for cv M. Jyoti and M. Janpriya, respectively, in 10% FAA soil and by 29.5% in cv M. Jagriti in 5% FAA soil. The experimental results depict that different cultivars of mung bean demonstrate a marked difference in response to various concentrations of fly ash under field conditions and this may indicate a genetic base for variability.
ABSTRACT An experiment was conducted to evaluate the combined effect of amended media and vermiwa... more ABSTRACT An experiment was conducted to evaluate the combined effect of amended media and vermiwash on growth and flowering of landscape gerbera grown under greenhouse condition. CPV and Soil amended with vermicompost, hydrogel and HBM had significant positive effects on growth and flowering of gerbera. Appearance of first true leaf in newly transplanted plants (3.67 d), number of leaves per plant at first flower appearance (16.33), appearance of first true leaf after harvest (4.00 days), leaf area per leaf (82.80 cm2), maximum number of primary roots (42.00), minimum days taken for appearance of first flower bud (49.67), minimum days taken for first flower harvest (71.67), flower head diameter (10.11 cm), days taken to flower senescence in plants (20.33), fresh and dry weight of plant was recorded on CPV+PHG+VC with 20% vermiwash sprayed. Compared to all other treatments chlorophyll content and relative water content significantly higher with vermicompost 20%, PHG 0.25% and HMB 1.0% with vermiwash 20% sprayed. The beneficial effects of amended media were associated with change in physical and chemical properties and hormone like effect of vermiwash which increase the growth of the plants. Therefore, it can be concluded that, plant performance was best in the media containing CPV+PHG+VC with 20% vermiwash concentration.
PKCε is a transforming oncogene and a predictive biomarker of various human cancers. However, a p... more PKCε is a transforming oncogene and a predictive biomarker of various human cancers. However, a precise in vivo link of PKCε to cancer induction, progression and metastasis remain undefined. To achieve these goals, we generated tissue specific conditional PKCε knockout mice (PKCε-CKO) using cre-lox technology. Homozygous PKCεLoxP/LoxP mice have normal body weight and phenotype. To determine what effect loss of PKCε would have on the prostate, the PKCεLoxP/LoxP mice were bred to probasin cre (PB-Cre4+) mice which express cre specifically in the prostate epithelium of postnatal mice. Western blot and immunohistochemical analyses showed reduced levels of PKCε specifically in the prostate of PKCε-CKO mice. Histopathological analyses of prostate from both PKCεLoxP/LoxP and prostate PKCε-CKO mice showed normal pathology. To determine the functional impact of prostate specific deletion of PKCε on prostate tumor growth, we performed an orthotopic xenograft study. Transgenic adenocarcinoma o...
Insulin/IGF-1-like signalling (IIS) and dietary restriction (DR) are the two major modulatory pat... more Insulin/IGF-1-like signalling (IIS) and dietary restriction (DR) are the two major modulatory pathways controlling longevity across species. Here, we show that both pathways license a common chromatin modifier, ZFP-1/AF10. The downstream transcription factors of the IIS and select DR pathways, DAF-16/FOXO or PHA-4/FOXA, respectively, both transcriptionally regulate the expression of zfp-1. ZFP-1, in turn, negatively regulates the expression of DAF-16/FOXO and PHA-4/FOXA target genes, apparently forming feed-forward loops that control the amplitude as well as the duration of gene expression. We show that ZFP-1 mediates this regulation by negatively influencing the recruitment of DAF-16/FOXO and PHA-4/FOXA to their target promoters. Consequently, zfp-1 is required for the enhanced longevity observed during DR and on knockdown of IIS. Our data reveal how two distinct sensor pathways control an overlapping set of genes, using different downstream transcription factors, integrating poten...
Chronic exposure to ultraviolet radiation (UVR) is linked to the development of cutaneous squamou... more Chronic exposure to ultraviolet radiation (UVR) is linked to the development of cutaneous squamous cell carcinoma (SCC), a non-melanoma form of skin cancer that can metastasize. Tumor necrosis factor-alpha (TNFα), a pro-inflammatory cytokine, is linked to UVR-induced development of SCC. To find clues about the mechanisms by which TNFα may promote UVR-induced development of SCC, we investigated changes in the expression profiling of microRNAs (miRNA), a novel class of short noncoding RNAs, which affects translation and stability of mRNAs. In this experiment, TNFα knockout (TNFα KO) mice and their wild type (WT) littermates were exposed to acute UVR (2.0 kJ/m2) and the expression profiling of epidermal miRNA was determined 4hr post UVR exposure. TNFα deletion in untreated WT mice resulted in differential expression (log fold change>1) of seventeen miRNA. UVR exposure in WT mice induced differential expression of 22 miRNA. However, UVR exposure in TNFα KO mice altered only two miRNA...
APA PsycNET Our Apologies! - The following features are not available with your current Browser c... more APA PsycNET Our Apologies! - The following features are not available with your current Browser configuration. - display, print, save, export, and email selected records - get My List count - save record to My List - get references ...
Protein kinase C epsilon (PKCε), a Ca,(2+)-independent phospholipid-dependent serine/threonine ki... more Protein kinase C epsilon (PKCε), a Ca,(2+)-independent phospholipid-dependent serine/threonine kinase, is among the six PKC isoforms (α,δ, ε, η, μ, ζ) expressed in both mouse and human skin. Epidermal PKCε level dictates the susceptibility of PKCε transgenic (TG) mice to the development of cutaneous squamous cell carcinomas (SCC) elicited either by repeated exposure to ultraviolet radiation (UVR) or by using the DMBA initiation-TPA tumor promotion protocol (Cancer Res, 2004, 64:7756-65). Histologically, SCC in TG mice, like human SCC, is poorly differentiated and metastatic. Our earlier studies to elucidate mechanisms of PKCε-mediated development of SCC, using either DMBA-TPA or UVR, indicated elevated release of cytokine TNFα. To determine whether TNFα is essential for the development of SCC in TG mice, we generated PKCε transgenic mice//TNFα-knockout (TG//TNFαKO) by crossbreeding TNFαKO with TG mice. We now present that deletion of TNFα in TG mice inhibited the development of SCC ...
Lowering insulin-IGF-1-like signalling (IIS) activates FOXO transcription factors (TF) to extend ... more Lowering insulin-IGF-1-like signalling (IIS) activates FOXO transcription factors (TF) to extend life span across species. To study the dynamics of FOXO chromatin occupancy under this condition in C. elegans, we report the first recruitment profile of endogenous DAF-16 and show that the response is conserved. DAF-16 predominantly acts as a transcriptional activator and binding within the 0.5 kb promoter-proximal region results in maximum induction of downstream targets that code for proteins involved in detoxification and longevity. Interestingly, genes that are activated under low IIS already have higher DAF-16 recruited to their promoters in WT. DAF-16 binds to variants of the FOXO consensus sequence in the promoter proximal regions of genes that are exclusively targeted during low IIS. We also define a set of 'core' direct targets, after comparing multiple studies, which tend to co-express and contribute robustly towards IIS-associated phenotypes. Additionally, we show th...
. It is commonly known as Jin Gang Teng in Chinese, Chobchini in Hindi, Madhusnuhi in Sanskrit an... more . It is commonly known as Jin Gang Teng in Chinese, Chobchini in Hindi, Madhusnuhi in Sanskrit and china root in English. It possesses antiinflammatory, diuretic, anti-diabetic, anti-psoriatic, digestive properties. There is no authentic protocol for the pharmacognostic parameters and anti-diabetic activity of Smilax china L. So, in the present study a detailed investigation of its pharmacognostic properties and anti-diabetic activity was performed. The pharmacognostic study included powder microscopy, loss of weight on drying, ash values, swelling index, and fluorescence activity. In addition, extracts were obtained using petroleum ether, chloroform, alcohol, hydroalcoholic, and aqueous solvents and the phytochemical constituents present in these extracts were subjected to a variety of chemical tests. Among these extracts, the hydroalcoholic and aqueous fractions exhibited anti-diabetic activity in rats with alloxan-induced diabetes.
Journal of experimental therapeutics & oncology, 2015
Etiopathology of tumor biology is highly complex and ovarian cancer is one of the important gynae... more Etiopathology of tumor biology is highly complex and ovarian cancer is one of the important gynaecological neoplasia associated with high risk of mortality rate. Methlenetetrahydrofolate reductase (MTHFR) mutation are commonly linked to folate metabolism with increased risk factor for the development of neural tube defects, recurrent pregnancy loss and development of several type of cancer but genetic interaction between two alleles of MTHFR has been poorly defined in ovarian cancer in India. Hence, present study becomes imperative with the aim to assess the alleles frequency of MTHFR (C677T & A1298C) gene polymorphism using PCR based RFLP analysis. The O.R at 95% confidence interval (C.I.) was computed between cases and their respective controls to determine "risk factor". Interestingly, our findings reveals highly significant (p < 0.001) difference in heterozygous (CT) condition of C677T allele by computing odd ratio (0.12 at 95% C.I, 0.021-0.0428; P for trend = 0.001...
We present here that heat-shock protein 90 (Hsp90) inhibitor 17-(allylamino)-17-demethoxygeldanam... more We present here that heat-shock protein 90 (Hsp90) inhibitor 17-(allylamino)-17-demethoxygeldanamycin (17AAG), when topically applied to mouse skin, inhibits UVR-induced development of cutaneous squamous cell carcinoma (SCC). In these experiments, DMSO:acetone (1:40 v/v) solution of 17AAG (500 nmol) was applied topically to mouse skin in conjunction with each UVR exposure (1.8 kJ m À 2 ). The UVR source was Kodacelfiltered FS-40 sun lamps (approximately 60% UVB and 40% UVA). In independent experiments with three separate mouse lines (SKH-1 hairless mice, wild-type FVB, and protein kinase C epsilon (PKCe)-overexpressing transgenic FVB mice), 17AAG treatment increased the latency and decreased both the incidence and multiplicity of UVRinduced SCC. Topical 17AAG alone or in conjunction with UVR treatments elicited neither skin nor systemic toxicity. 17AAG-caused inhibition of SCC induction was accompanied by a decrease in UVR-induced (1) hyperplasia, (2) Hsp90b-PKCe interaction, and (3) expression levels of Hsp90b, Stat3, pStat3Ser727, pStat3Tyr705, pAktSer473, and matrix metalloproteinase (MMP). The results presented here indicate that topical Hsp90 inhibitor 17AAG is effective in prevention of UVR-induced epidermal hyperplasia and SCC. One may conclude from the preclinical data presented here that topical 17AAG may be useful for prevention of UVR-induced inflammation and cutaneous SCC either developed in UVR-exposed or organ transplant population.
Recent patents on drug delivery & formulation, Jan 29, 2015
Fast Dissolving/Disintegrating Dosage Forms (FDDFs) are formulated and developed for immediate dr... more Fast Dissolving/Disintegrating Dosage Forms (FDDFs) are formulated and developed for immediate drug delivery, quicker onset of action, and/or as age appropriate dosage forms meant for special populations like paediatrics, , psychotic, dysphagic, and frequent traveller patients. FDDFs family include tablets (FDTs), oral films (FDOF), mini tablets, capsules, pellets, granules etc. FDDFs dissolve/disintegrate quickly in saliva resulting in solution/suspension for oral ingestion and thereby eliminate the need for water for swallowing the dosage form.FDTs market, in regions of US, Europe, and Japan, grew more than $8.4 billion in 2011 and is expected to reach to $12 billion globally by 2018[1]. FDOF market is expected to attain $1 billion mark in 2015 [1]. FDDFs have successfully entered in Pharmacopoeia as Orally Disintegrating Tablets (US and Japan) or Orodispersible Tablets (ODT or OD Tablet) [Europe and Japan] and orodispersible films (ODF) [Europe].FDDFs have recently shifted their ...
Proper disposal and/or recycling of different industrial waste materials have long been recognize... more Proper disposal and/or recycling of different industrial waste materials have long been recognized as a prime environmental concern throughout the world, and fly ash is major amongst them. In the present study, we tried to assess the feasibilities of possible effective and safe utilization of fly ash as soil amendment in Indian paddy field and its impact on rice plants, especially at growth and yield level. Our results showed that certain doses of fly ash amendments have significantly improved the physico-chemical and mineralogical properties of paddy field soil, and at lower level of amendments, fly ash induced the growth performances of three rice cultivars too. Grain yield and grain quality also responded similarly as per the growth responses. However, differential cultivar response was observed accordingly, and cultivar Sugandha-3 showed higher yield as compared with cultivars Sambha and Saryu-52. Based on the observed results, it was concluded that up to a certain level, fly ash amendments could be beneficial for Indian paddy field and can be utilized as feasible management strategy for the disposal of this major industrial waste.
Journal of clinical and diagnostic research : JCDR, 2014
Pre-Eclampsia (PE) affects 6-31% of pregnant women with multiple gestations. There are conflictin... more Pre-Eclampsia (PE) affects 6-31% of pregnant women with multiple gestations. There are conflicting reports on the association of PE with Chorionicity and zygosity; however, there is a lack of information on this potential association in a population of pregnant Asian Indian women. To determine as to whether chorionicity and zygosity were associated with PE in a population of Asian Indian women with twin gestations. A retrospective observational study was done at a single tertiary care centre in Southern India. The study included pregnant women with twin gestations, who was delivered at the study institute in 2012. Hypertension in pregnancy was categorized, based on the criteria of the International Society for the Study of Hypertension in Pregnancy. Chorionicity was determined by using ultrasonography and zygosity was determined, based on clinical criteria. Point estimates and the 95% Confidence Intervals (CI) around point estimates of PE and associations of chorionicity and zygosit...
Field experiments were conducted to evaluate the effect of fly ash on growth and yield of three l... more Field experiments were conducted to evaluate the effect of fly ash on growth and yield of three locally grown cultivars of an important leguminous plant mung bean (Vigna radiata L.) on soil amended with different concentrations of fly ash. The values of pH, EC, WHC, soil cations, total heavy metals of the soil increased; however, values of BD, NH(4)(+)-N, NO(3)(-)-N, total N, organic carbon (OC), organic matter (OM), available P and cation exchange capacity (CEC) decreased with fly ash incorporation in the soil. Fly ash amendment led to improve the growth performance and various yield attributes. An increase in all the growth parameters was recorded in 10% fly ash amended (FAA) soil for cv M. Jyoti and M. Janpriya, while 5% FAA soil was most suitable for cv M. Jagriti. Yield (gm(-2)) also increased significantly by 40.6% and 33.9% for cv M. Jyoti and M. Janpriya, respectively, in 10% FAA soil and by 29.5% in cv M. Jagriti in 5% FAA soil. The experimental results depict that different cultivars of mung bean demonstrate a marked difference in response to various concentrations of fly ash under field conditions and this may indicate a genetic base for variability.
ABSTRACT An experiment was conducted to evaluate the combined effect of amended media and vermiwa... more ABSTRACT An experiment was conducted to evaluate the combined effect of amended media and vermiwash on growth and flowering of landscape gerbera grown under greenhouse condition. CPV and Soil amended with vermicompost, hydrogel and HBM had significant positive effects on growth and flowering of gerbera. Appearance of first true leaf in newly transplanted plants (3.67 d), number of leaves per plant at first flower appearance (16.33), appearance of first true leaf after harvest (4.00 days), leaf area per leaf (82.80 cm2), maximum number of primary roots (42.00), minimum days taken for appearance of first flower bud (49.67), minimum days taken for first flower harvest (71.67), flower head diameter (10.11 cm), days taken to flower senescence in plants (20.33), fresh and dry weight of plant was recorded on CPV+PHG+VC with 20% vermiwash sprayed. Compared to all other treatments chlorophyll content and relative water content significantly higher with vermicompost 20%, PHG 0.25% and HMB 1.0% with vermiwash 20% sprayed. The beneficial effects of amended media were associated with change in physical and chemical properties and hormone like effect of vermiwash which increase the growth of the plants. Therefore, it can be concluded that, plant performance was best in the media containing CPV+PHG+VC with 20% vermiwash concentration.
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