Papers by Esmeralda Benitez
Journal of Infectious Diseases, 2009
Human infections with Ascaris lumbricoides may have important effects on allergy and susceptibili... more Human infections with Ascaris lumbricoides may have important effects on allergy and susceptibility to infectious diseases that start in early life. To investigate if sensitization to Ascaris occurs in utero, we measured IFN-γ and IL-4 responses in Ascaris antigen-stimulated cord blood of newborns of infected and non-infected mothers using flow cytometry. There was evidence of elevated frequencies of IFN-γ and IL-4-expressing CD4+ T cells in newborns of infected mothers compared to those of non-infected mothers. Our data provide evidence of in utero sensitization to A. lumbricoides, and raise the possibility that the immunological effects of infection start in the fetus. , MO) at 10 μg /mL in a humidified atmosphere of 5% CO 2 at 37°C. After 2 hours, 20 μg/mL of Brefeldin A (Sigma) was added to each culture well and cultures were Guadalupe et al.
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Papers by Esmeralda Benitez