OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety pro... more OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety profile, widely used to prevent recurrent respiratory tract infections. Several studies have been reporting the immunomodulating properties and antiviral roles of OM-85. The COVID-19 pandemic, originating in 2019, has presented a significant global public health crisis. While effective vaccines have been developed, vaccination rates vary considerably, and numerous concerning viral variants continue to emerge. The challenge persists in creating early interventions to halt the progression of the disease to its severe stages. To examine the therapeutic effect of OM-85 after SARS-CoV-2 infection and compared to recombinant human (rhINF-β) we collected nasopharyngeal cells from COVID-19 patients. The cells were treated ex-vivo with OM-85 or hrINF-β and the response was analyzed after 24h for gene expression by real-time PCR. We found that OM-85 decreased the SARS-CoV-2 N1 gene expression and inc...
bioRxiv (Cold Spring Harbor Laboratory), Oct 14, 2022
We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself an... more We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 and MLKL) were involved in SARS-CoV-2 N1 gene expression in monocytes. SARS-CoV-2 immune complexes promoted monocyte necroptosis in a RIPK3-and MLKL-dependent manner, and Syk tyrosine kinase was necessary for SARS-CoV-2 immune complex-induced monocyte necroptosis, indicating the involvement of Fcγ receptors on necroptosis. Finally, we provide evidence that elevated LDH levels as a marker of lytic cell death are associated with COVID-19 pathogenesis.
O laser de baixa potência vem sendo utilizado como adjuvante no tratamento de feridas por acelera... more O laser de baixa potência vem sendo utilizado como adjuvante no tratamento de feridas por acelerar o processo cicatricial, mediante o aumento do metabolismo celular. O interesse pelo uso do laser nas aplicações clínicas e o potencial das interações não-térmicas da luz laser com o tecido vem sendo motivo de vários estudos. O potencial da irradiação laser de intensidade relativamente baixa aplicada diretamente no tecido para modular certos processos biológicos, em particular fotobioestimular os processos de regeneração dos tecidos, controlar o processo inflamatório e atenuar a dor. O objetivo deste trabalho é discutir a eficácia do laser através de um levantamento literário. Os métodos utilizados para realização deste trabalho foram bases de dados da bireme; scielo; bvsalud; pubmed; portal da saúde. gov; bdtd; cochrane; com as palavras chaves: ferida, laserterapia de baixa intensidade, inflamação, regeneração tecidual, reparo tecidual, terapia, dor, laser de baixa potência; cicatriza...
Objetivo: Identificar, com base nas publicações científicas, os resultados do uso da laserterapia... more Objetivo: Identificar, com base nas publicações científicas, os resultados do uso da laserterapia de baixa intensidade no tratamento de feridas. Método:Trata-se de uma pesquisa descritiva tipo revisão integrativa da literatura (RIL). A pesquisa foi realizada por meio de busca eletrônica nas bases de dados: Banco de Dados em Enfermagem (BDENF), Scientific Electronic Library Online (SciELO), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine (PUBMED) e Biblioteca Virtual em Saúde (BVS), considerando o recorte temporal de 2007 a 2022. Resultados e Conclusão: A maioria dos estudos avaliados, mostraram resultados estatisticamente significativos em variáveis como tempo de cicatrização e fechamento da ferida p=<0,002. Outro desfecho significativo foi para o alívio da dor p=<0,001, mostrando efeitos analgésicos. Descritores: Laserterapia AND Feridas e Lesões
OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively b... more OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively breastfeed for the first 6 months of life in a sample of women in the first 24 h postpartum during the hospital stay. METHODS Cross-sectional study with data from screening phase of a birth cohort. The proportion of mothers who did not intend to breastfeed exclusively for 6 months (primary outcome) derived from a negative response to the question "Would you be willing to try to breastfeed exclusively for the first 6 months?", in an interview conducted by previously trained interviewers. Crude and adjusted prevalence ratios (PR) with 95% confidence intervals were obtained by Poisson regression with robust variance. RESULTS A total of 2964 postpartum women were interviewed. The overall prevalence of mothers who did not intend to breastfeed exclusively for 6 months was 17.8% (16.4-19.1%). After adjusting for maternal age and type of pregnancy (singleton or multiple), no intention to exclusively breastfeed was higher in mothers with a monthly household income < 3 minimum wages (PR, 1.64; 1.35-1.98) and in those who intended to smoke 4-7 days/week after delivery (PR, 1.42; 1.11-1.83). The presence of significant newborn morbidity (PR, 0.32; 0.19-0.54) and intention to breastfeed up to 12 months (PR, 0.46; 0.38-0.55) had a protective effect against not intending to breastfeed exclusively for 6 months. CONCLUSIONS Approximately 1 in every 5 mothers did not intend to breastfeed exclusively for 6 months. Strategies aimed at promoting exclusive breastfeeding should focus attention on mothers from lower economic strata and smokers.
Respiratory syncytial virus (RSV) is a seasonal pathogen responsible for the highest percentage o... more Respiratory syncytial virus (RSV) is a seasonal pathogen responsible for the highest percentage of viral bronchiolitis in pediatric patients. There are currently no vaccine available and therapeutic methods to mitigate the severity of RSV bronchiolitis are limited. OM-85, an oral standardized bacterial lysate isolated from human respiratory strains and widely used to prevent recurrent infections and/or exacerbations in populations at risk, has been shown to be effective and safe in children and adults. Here, we demonstrate that airway administration of OM-85 in Balb/c mice prior to infection prevents RSV-induced disease, resulting in inhibition of viral replication associated with less perivascular and peribronchial inflammation in the lungs. These protective effects are dose and time-dependent with complete protection using 1mg dose of OM-85 only four times intranasally. Mechanistic insights using this topical route in the airways revealed increased alveolar macrophages, a selectiv...
OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety pro... more OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety profile, widely used to prevent recurrent respiratory tract infections. Several studies have been reporting the antiviral roles of OM-85. Here we demonstrated the effect of ex-vivo OM-85 exposure in nasopharyngeal cells collected from COVID-19 patients. OM-85 decreased the SARS-CoV-2 N1 gene expression and increased RIG-I (DDX58) gene expression in these cells. These data support the antiviral effect of OM-85 against SARS-CoV-2.
Background Gut microbiota-derived short-chain fatty-acid (SFCA) acetate protects mice against RSV... more Background Gut microbiota-derived short-chain fatty-acid (SFCA) acetate protects mice against RSV A2 strain infection by increasing interferon-β production and expression of interferon-stimulated genes (ISGs). However, the role of SFCA in RSV infection using strains isolated from patients is unknown. Methods We first used RSV clinical strains isolated from infants hospitalized with RSV bronchiolitis to investigate the effects of in vitro SCFA-acetate treatment of human pulmonary epithelial cells. We next examined whether SCFA-acetate treatment is beneficial in a mouse model of RSV infection using clinical isolates. We sought to investigate the relationship of gut microbiota and fecal acetate with disease severity among infants hospitalized with RSV bronchiolitis, and whether treating their respiratory epithelial cells with SCFA-acetate ex-vivo impacts viral load and ISG expression. We further treated epithelial cells from SARS-CoV-2 infected patients with SCFA-acetate. Findings In vitro pre-treatment of A549 cells with SCFA-acetate reduced RSV infection with clinical isolates and increased the expression of RIG-I and ISG15. Animals treated with SCFA-acetate intranasally recovered significantly faster, with reduction in the RSV clinical isolates viral load, and increased lung expression of IFNB1 and the RIG-I. Experiments in RIG-I knockout A549 cells demonstrated that the protection relies on RIG-I presence. Gut microbial profile was associated with bronchiolitis severity and with acetate in stool. Increased SCFA-acetate levels were associated with increasing oxygen saturation at admission, and shorter duration of fever. Ex-vivo treatment of patients’ respiratory cells with SCFA-acetate reduced RSV load and increased expression of ISGs OAS1 and ISG15, and virus recognition receptors MAVS and RIG-I, but not IFNB1. These SCFA-acetate effects were not found on cells from SARS-CoV-2 infected patients. Interpretation SCFA-acetate reduces the severity of RSV infection and RSV viral load through modulation of RIG-I expression. Funding FAPERGS (FAPERGS/MS/CNPq/SESRS no. 03/2017 - PPSUS 17/2551-0001380-8 and COVID-19 20/2551-0000258-6); CNPq 312504/2017-9; CAPES) - Finance Code 001.
Objetivo: Conhecer a percepção do pai frente ao nascimento do filho em uma maternidade pública de... more Objetivo: Conhecer a percepção do pai frente ao nascimento do filho em uma maternidade pública de um Hospital do Rio Grande do Sul. Métodos: Estudo descritivo e qualitativo, realizado com pais durante o trabalho de parto e parto da sua companheira em um Alojamento Conjunto, utilizando entrevistas semiestruturadas para coleta de dados durante os meses de agosto e novembro de 2020. Resultados: Participaram do estudo 11 pais, com idade prevalente entre 20-30 anos, a maioria tem como grau de instrução ensino fundamental incompleto, a procedência de Parobé e o estado civil casado. O número de parto cesárea foi maior e 73% dos partos foram assistidos. Os achados do estudo propuseram refletir e discutir quanto a emoção e felicidade ao nascimento do filho, a satisfação com o atendimento da equipe, ansiedade e preocupação com o parto, expectativas com o nascimento, a participação durante o trabalho de parto e parto e a segurança em se tornar pai. Conclusão: Podemos evidenciar a importância e...
OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively b... more OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively breastfeed for the first 6 months of life in a sample of women in the first 24 h postpartum during the hospital stay. METHODS Cross-sectional study with data from screening phase of a birth cohort. The proportion of mothers who did not intend to breastfeed exclusively for 6 months (primary outcome) derived from a negative response to the question "Would you be willing to try to breastfeed exclusively for the first 6 months?", in an interview conducted by previously trained interviewers. Crude and adjusted prevalence ratios (PR) with 95% confidence intervals were obtained by Poisson regression with robust variance. RESULTS A total of 2964 postpartum women were interviewed. The overall prevalence of mothers who did not intend to breastfeed exclusively for 6 months was 17.8% (16.4-19.1%). After adjusting for maternal age and type of pregnancy (singleton or multiple), no intention t...
Objetivo: Identificar, sintetizar e analisar as literaturas referentes ao cuidado com COVID-19 na... more Objetivo: Identificar, sintetizar e analisar as literaturas referentes ao cuidado com COVID-19 na gestação. Métodos: Revisão integrativa nas bases de dados Medline e Epistemonikos utilizando os descritores: “coronavírus na gestação” e “COVID-19 na gestação”. A busca ocorreu em abril de 2020. Resultados: Foram selecionados 26 artigos à qual emergiram três categorias: Diagnósticos de COVID-19 em gestantes e precauções; Cuidados com gestantes com COVID-19; Cuidados com fetos e neonatos. Os sinais e sintomas em mulheres grávidas com COVID-19 tendem a ser leves ou moderadas, possivelmente como resultado dos efeitos combinados do gênero, idade e estado imunológico da gravidez. De acordo com a literatura a principal via de parto escolhida para as gestantes acometidas pelo coronavírus é o parto normal. Em trabalhos de parto, a segunda fase deve ser, se possível, adiantada, pois a dificuldade respiratória da gestante pode ser agravar devido ao esforço e utilização de máscara. Considerações ...
OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety pro... more OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety profile, widely used to prevent recurrent respiratory tract infections. Several studies have been reporting the immunomodulating properties and antiviral roles of OM-85. The COVID-19 pandemic, originating in 2019, has presented a significant global public health crisis. While effective vaccines have been developed, vaccination rates vary considerably, and numerous concerning viral variants continue to emerge. The challenge persists in creating early interventions to halt the progression of the disease to its severe stages. To examine the therapeutic effect of OM-85 after SARS-CoV-2 infection and compared to recombinant human (rhINF-β) we collected nasopharyngeal cells from COVID-19 patients. The cells were treated ex-vivo with OM-85 or hrINF-β and the response was analyzed after 24h for gene expression by real-time PCR. We found that OM-85 decreased the SARS-CoV-2 N1 gene expression and inc...
bioRxiv (Cold Spring Harbor Laboratory), Oct 14, 2022
We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself an... more We analyzed the ability of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) itself and SARS-CoV-2-IgG immune complexes to trigger human monocyte necroptosis. SARS-CoV-2 was able to induce monocyte necroptosis dependently of MLKL activation. Necroptosis-associated proteins (RIPK1, RIPK3 and MLKL) were involved in SARS-CoV-2 N1 gene expression in monocytes. SARS-CoV-2 immune complexes promoted monocyte necroptosis in a RIPK3-and MLKL-dependent manner, and Syk tyrosine kinase was necessary for SARS-CoV-2 immune complex-induced monocyte necroptosis, indicating the involvement of Fcγ receptors on necroptosis. Finally, we provide evidence that elevated LDH levels as a marker of lytic cell death are associated with COVID-19 pathogenesis.
O laser de baixa potência vem sendo utilizado como adjuvante no tratamento de feridas por acelera... more O laser de baixa potência vem sendo utilizado como adjuvante no tratamento de feridas por acelerar o processo cicatricial, mediante o aumento do metabolismo celular. O interesse pelo uso do laser nas aplicações clínicas e o potencial das interações não-térmicas da luz laser com o tecido vem sendo motivo de vários estudos. O potencial da irradiação laser de intensidade relativamente baixa aplicada diretamente no tecido para modular certos processos biológicos, em particular fotobioestimular os processos de regeneração dos tecidos, controlar o processo inflamatório e atenuar a dor. O objetivo deste trabalho é discutir a eficácia do laser através de um levantamento literário. Os métodos utilizados para realização deste trabalho foram bases de dados da bireme; scielo; bvsalud; pubmed; portal da saúde. gov; bdtd; cochrane; com as palavras chaves: ferida, laserterapia de baixa intensidade, inflamação, regeneração tecidual, reparo tecidual, terapia, dor, laser de baixa potência; cicatriza...
Objetivo: Identificar, com base nas publicações científicas, os resultados do uso da laserterapia... more Objetivo: Identificar, com base nas publicações científicas, os resultados do uso da laserterapia de baixa intensidade no tratamento de feridas. Método:Trata-se de uma pesquisa descritiva tipo revisão integrativa da literatura (RIL). A pesquisa foi realizada por meio de busca eletrônica nas bases de dados: Banco de Dados em Enfermagem (BDENF), Scientific Electronic Library Online (SciELO), Literatura Latino-Americana e do Caribe em Ciências da Saúde (LILACS), US National Library of Medicine (PUBMED) e Biblioteca Virtual em Saúde (BVS), considerando o recorte temporal de 2007 a 2022. Resultados e Conclusão: A maioria dos estudos avaliados, mostraram resultados estatisticamente significativos em variáveis como tempo de cicatrização e fechamento da ferida p=<0,002. Outro desfecho significativo foi para o alívio da dor p=<0,001, mostrando efeitos analgésicos. Descritores: Laserterapia AND Feridas e Lesões
OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively b... more OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively breastfeed for the first 6 months of life in a sample of women in the first 24 h postpartum during the hospital stay. METHODS Cross-sectional study with data from screening phase of a birth cohort. The proportion of mothers who did not intend to breastfeed exclusively for 6 months (primary outcome) derived from a negative response to the question "Would you be willing to try to breastfeed exclusively for the first 6 months?", in an interview conducted by previously trained interviewers. Crude and adjusted prevalence ratios (PR) with 95% confidence intervals were obtained by Poisson regression with robust variance. RESULTS A total of 2964 postpartum women were interviewed. The overall prevalence of mothers who did not intend to breastfeed exclusively for 6 months was 17.8% (16.4-19.1%). After adjusting for maternal age and type of pregnancy (singleton or multiple), no intention to exclusively breastfeed was higher in mothers with a monthly household income < 3 minimum wages (PR, 1.64; 1.35-1.98) and in those who intended to smoke 4-7 days/week after delivery (PR, 1.42; 1.11-1.83). The presence of significant newborn morbidity (PR, 0.32; 0.19-0.54) and intention to breastfeed up to 12 months (PR, 0.46; 0.38-0.55) had a protective effect against not intending to breastfeed exclusively for 6 months. CONCLUSIONS Approximately 1 in every 5 mothers did not intend to breastfeed exclusively for 6 months. Strategies aimed at promoting exclusive breastfeeding should focus attention on mothers from lower economic strata and smokers.
Respiratory syncytial virus (RSV) is a seasonal pathogen responsible for the highest percentage o... more Respiratory syncytial virus (RSV) is a seasonal pathogen responsible for the highest percentage of viral bronchiolitis in pediatric patients. There are currently no vaccine available and therapeutic methods to mitigate the severity of RSV bronchiolitis are limited. OM-85, an oral standardized bacterial lysate isolated from human respiratory strains and widely used to prevent recurrent infections and/or exacerbations in populations at risk, has been shown to be effective and safe in children and adults. Here, we demonstrate that airway administration of OM-85 in Balb/c mice prior to infection prevents RSV-induced disease, resulting in inhibition of viral replication associated with less perivascular and peribronchial inflammation in the lungs. These protective effects are dose and time-dependent with complete protection using 1mg dose of OM-85 only four times intranasally. Mechanistic insights using this topical route in the airways revealed increased alveolar macrophages, a selectiv...
OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety pro... more OM-85 is a bacterial lysate from common respiratory tract pathogens, with an excellent safety profile, widely used to prevent recurrent respiratory tract infections. Several studies have been reporting the antiviral roles of OM-85. Here we demonstrated the effect of ex-vivo OM-85 exposure in nasopharyngeal cells collected from COVID-19 patients. OM-85 decreased the SARS-CoV-2 N1 gene expression and increased RIG-I (DDX58) gene expression in these cells. These data support the antiviral effect of OM-85 against SARS-CoV-2.
Background Gut microbiota-derived short-chain fatty-acid (SFCA) acetate protects mice against RSV... more Background Gut microbiota-derived short-chain fatty-acid (SFCA) acetate protects mice against RSV A2 strain infection by increasing interferon-β production and expression of interferon-stimulated genes (ISGs). However, the role of SFCA in RSV infection using strains isolated from patients is unknown. Methods We first used RSV clinical strains isolated from infants hospitalized with RSV bronchiolitis to investigate the effects of in vitro SCFA-acetate treatment of human pulmonary epithelial cells. We next examined whether SCFA-acetate treatment is beneficial in a mouse model of RSV infection using clinical isolates. We sought to investigate the relationship of gut microbiota and fecal acetate with disease severity among infants hospitalized with RSV bronchiolitis, and whether treating their respiratory epithelial cells with SCFA-acetate ex-vivo impacts viral load and ISG expression. We further treated epithelial cells from SARS-CoV-2 infected patients with SCFA-acetate. Findings In vitro pre-treatment of A549 cells with SCFA-acetate reduced RSV infection with clinical isolates and increased the expression of RIG-I and ISG15. Animals treated with SCFA-acetate intranasally recovered significantly faster, with reduction in the RSV clinical isolates viral load, and increased lung expression of IFNB1 and the RIG-I. Experiments in RIG-I knockout A549 cells demonstrated that the protection relies on RIG-I presence. Gut microbial profile was associated with bronchiolitis severity and with acetate in stool. Increased SCFA-acetate levels were associated with increasing oxygen saturation at admission, and shorter duration of fever. Ex-vivo treatment of patients’ respiratory cells with SCFA-acetate reduced RSV load and increased expression of ISGs OAS1 and ISG15, and virus recognition receptors MAVS and RIG-I, but not IFNB1. These SCFA-acetate effects were not found on cells from SARS-CoV-2 infected patients. Interpretation SCFA-acetate reduces the severity of RSV infection and RSV viral load through modulation of RIG-I expression. Funding FAPERGS (FAPERGS/MS/CNPq/SESRS no. 03/2017 - PPSUS 17/2551-0001380-8 and COVID-19 20/2551-0000258-6); CNPq 312504/2017-9; CAPES) - Finance Code 001.
Objetivo: Conhecer a percepção do pai frente ao nascimento do filho em uma maternidade pública de... more Objetivo: Conhecer a percepção do pai frente ao nascimento do filho em uma maternidade pública de um Hospital do Rio Grande do Sul. Métodos: Estudo descritivo e qualitativo, realizado com pais durante o trabalho de parto e parto da sua companheira em um Alojamento Conjunto, utilizando entrevistas semiestruturadas para coleta de dados durante os meses de agosto e novembro de 2020. Resultados: Participaram do estudo 11 pais, com idade prevalente entre 20-30 anos, a maioria tem como grau de instrução ensino fundamental incompleto, a procedência de Parobé e o estado civil casado. O número de parto cesárea foi maior e 73% dos partos foram assistidos. Os achados do estudo propuseram refletir e discutir quanto a emoção e felicidade ao nascimento do filho, a satisfação com o atendimento da equipe, ansiedade e preocupação com o parto, expectativas com o nascimento, a participação durante o trabalho de parto e parto e a segurança em se tornar pai. Conclusão: Podemos evidenciar a importância e...
OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively b... more OBJECTIVE To investigate the prevalence and factors associated with no intention to exclusively breastfeed for the first 6 months of life in a sample of women in the first 24 h postpartum during the hospital stay. METHODS Cross-sectional study with data from screening phase of a birth cohort. The proportion of mothers who did not intend to breastfeed exclusively for 6 months (primary outcome) derived from a negative response to the question "Would you be willing to try to breastfeed exclusively for the first 6 months?", in an interview conducted by previously trained interviewers. Crude and adjusted prevalence ratios (PR) with 95% confidence intervals were obtained by Poisson regression with robust variance. RESULTS A total of 2964 postpartum women were interviewed. The overall prevalence of mothers who did not intend to breastfeed exclusively for 6 months was 17.8% (16.4-19.1%). After adjusting for maternal age and type of pregnancy (singleton or multiple), no intention t...
Objetivo: Identificar, sintetizar e analisar as literaturas referentes ao cuidado com COVID-19 na... more Objetivo: Identificar, sintetizar e analisar as literaturas referentes ao cuidado com COVID-19 na gestação. Métodos: Revisão integrativa nas bases de dados Medline e Epistemonikos utilizando os descritores: “coronavírus na gestação” e “COVID-19 na gestação”. A busca ocorreu em abril de 2020. Resultados: Foram selecionados 26 artigos à qual emergiram três categorias: Diagnósticos de COVID-19 em gestantes e precauções; Cuidados com gestantes com COVID-19; Cuidados com fetos e neonatos. Os sinais e sintomas em mulheres grávidas com COVID-19 tendem a ser leves ou moderadas, possivelmente como resultado dos efeitos combinados do gênero, idade e estado imunológico da gravidez. De acordo com a literatura a principal via de parto escolhida para as gestantes acometidas pelo coronavírus é o parto normal. Em trabalhos de parto, a segunda fase deve ser, se possível, adiantada, pois a dificuldade respiratória da gestante pode ser agravar devido ao esforço e utilização de máscara. Considerações ...
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