Hydrodistillation of the dried leaves of eleven species of the genus Eucalyptus L'Hér., i.e.,... more Hydrodistillation of the dried leaves of eleven species of the genus Eucalyptus L'Hér., i.e., E. astringens Maiden, E. camaldulensis Dehnh., E. diversifolia Bonpl., E. falcata Turcz., E. ficifolia F. Muell., E. gomphocephala DC., E. lehmannii (Schauer) Benth., E. maculata Hook., E. platypus Hook., E. polyanthemos Schauer, and E. rudis Endl., harvested from Korbous arboreta (region of Nabeul, northeast of Tunisia) in April 2006, afforded essential oils in yields varying from 0.1±0.1 to 3.8±0.1%, dependent on the species. E. astringens and E. ficifolia showed the highest and the lowest mean percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 138 components, representing 74.0 to 99.1% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8‐cineole, followed by trans‐pinocarveol (1), spathulenol (2), α‐pinene, p‐cymene, (E,E)‐farnesol, cry...
Hydrodistillation of the dried leaves of 13 species of the genus Eucalyptus L&amp... more Hydrodistillation of the dried leaves of 13 species of the genus Eucalyptus L' Hér., viz., E. bicostata Maiden, Blakely & Simmonds, E. cinerea F. Muell. ex Benth., E. exerta F. Muell., E. gigantea Hook. f., E. gunnii Hook. f., E. macarthurii Deane & Maiden., E. macrorrhyncha F. Muell., E. maidenii F. Muell., E. odorata Behr., E. pauciflora Sieber ex Sprengel, E. sideroxylon A. Cunn. ex Woolls, E. tereticornis Sm., and E. viminalis Labill., harvested from Souinet arboreta (region of Ain Draaham, north of Tunisia) in June 2006, afforded essential oils in yields varying from 0.5+/-0.2 to 3.9+/-0.4%, dependent on the species. E. cinerea and E. exerta provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 142 components, representing 81.5 to 98.9% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8-cineole (1), followed by cryptone, spathulenol (4), p-cymene (2), viridiflorol (6), globulol (7), beta-eudesmol, alpha-terpineol (5), limonene (8), D-piperitone, alpha-pinene (3), cuminal, and gamma-eudesmol. The principal component and the hierarchical cluster analyses separated the 13 Eucalyptus leaf essential oils into three groups, each constituting a chemotype.
This study is designed to examine the chemical composition and antioxidant activity of the essent... more This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50-99.57% of the oils. Major components of the oils were α-pinene (58.61-77.39%), camphene (0.67-9.31%), δ-3-carene (0-10.01%) and trans-verbenol (0-5.24%). Antioxidant activities were determined by two different test systems, DPPH and ABTS radical scavenging activities. In both systems ripe berries exhibited better activity potential than the unripe ones.
Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e.... more Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e., E. brockwayi C. A. Gardn., E. gracilis F. Muell., E. gillii Maiden, E. largiflorens F. Muell., E. loxophleba Benth., E. occidentalis Endl., E. oldfieldii F. Muell., E. salmonophloia F. Muell., E. sargentii Maiden, E. stricklandii Maiden, E. torquata Luehm., and E. woodwardii Maiden, harvested from Hajeb Layoun arboreta (region of Kairouan, central Tunisia) in January 2005, afforded essential oils in yields varying from 0.5±0.1 to 5.7±0.5%, dependent on the species. E. sargentii and E. brockwayi provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 133 components, representing 92.9–98.8% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8‐cineole, terpinen‐4‐ol, α‐pinene (2), p‐cymene, aromadendrene (1), globulol (5), trans‐pinocarveol (6), spathulenol (7), β‐eudesmol, torquatone (3), and 4‐methylpentan‐2‐yl acetate (8). The principal component analysis and the hierarchical clustering indicated that the volatile leaf oil composition of the twelve Eucalyptus species could be clearly differentiated.
Stems, leaves, buds and flowers of Laurus nobilis L. growing wild in Tunisia were analysed for th... more Stems, leaves, buds and flowers of Laurus nobilis L. growing wild in Tunisia were analysed for their essential oil composition. The essential oil of Laurus nobilis L. gathered from different stations were isolated by hydrodistillation and analysed by GC/MS. The oil yields on a dry weight basis ranged between 0.4% and 1.1%. The major component identified was 1,8-cineole, other predominant components were alpha-terpinyl acetate, methyl eugenol, eugenol and linalool. Although the same compounds were present in all plant organs, the leaves differed from the stems in the concentration of 1,8-cineole and methyl eugenol, buds and flowers in the concentration of 1,8-cineole and the stem's oil composition differs from the others in content of methyl eugenol. The results obtained from GC/MS analysis of the volatile oils from individual plant organs were submitted to principal component analysis. Chemometric investigations led to differentiation of stems, leaves and buds-flowers with the respect to the content of 1,8-cineole, metyhyl eugenol and alpha-terpynil acetate; flowers and buds were non-differentiated. Finally, the antibacterial activity of the leaves' essential oils has been assayed.
Present study has been carried out to estimate the impact of geographical distribution on the yie... more Present study has been carried out to estimate the impact of geographical distribution on the yield and chemical constitute of three Eucalyptus verities viz E. cinerea F. Muell. ex Benth., E. astringens Maiden and E. sideroxylon A.Cunn. ex Schauer-. These species were collected from six arboreta of Tunisia in January 2008. The essential oil was extracted by hydrodistillation method and estimated the essential oil yield which varies from 1.5±0.1% to 4.0±0.2%. Results of the study revealed that yield of essential oil are not only depends on the Eucalyptus species but also depends on the origin of harvest. E. sideroxylon A. Cunn. exWoolls, cultivated in jbel abderrahman arboreta and E. cinerea F. Muell. ex Benth. from choucha (sejnanae) arboreta provided the lowest and the highest percentage of essential oil amongst all the studied provenances, respectively. GC (RI) and GC/MS analysis showed the presence of 163 components, representing 98.8 to 99.5% of the total oil. The contents of th...
This study is outlined to probe the chemical composition of essential oil and in vitro antioxidan... more This study is outlined to probe the chemical composition of essential oil and in vitro antioxidant activity of Juniperus oxycedrus ssp. macrocarpa (S. & m.) Ball. and Juniperus oxycedrus L. ssp. rufescens (L. K.) berries, collected from four sites, according to their maturity phase. The chemical composition of the hydrodistilled essential oil was analysed by GC-MS. Forty-eight compounds were identified, accounting for approximately 79.8-98.9% of the oil. The main constituents were α-pinene, germacrene D, myrcene, abietadiene and cis-calamenene, their mean percentage vary according to their phenological stage. The antioxidant activity of the samples was determined by the ABTS and DPPH radical scavenging activities. Hawaria essential oil extracted from mature berries showed the highest antioxidant capacity.
Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e.... more Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e., E. brockwayi C. A. Gardn., E. gracilis F. Muell., E. gillii Maiden, E. largiflorens F. Muell., E. loxophleba Benth., E. occidentalis Endl., E. oldfieldii F. Muell., E. salmonophloia F. Muell., E. sargentii Maiden, E. stricklandii Maiden, E. torquata Luehm., and E. woodwardii Maiden, harvested from Hajeb Layoun arboreta (region of Kairouan, central Tunisia) in January 2005, afforded essential oils in yields varying from 0.5±0.1 to 5.7±0.5%, dependent on the species. E. sargentii and E. brockwayi provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 133 components, representing 92.9–98.8% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8‐cineole, terpinen‐4‐ol, α‐pinene (2), p‐cymene, aromadendrene (1), g...
Hydrodistillation of the dried leaves of five species of the genus Eucalyptus L He¤ r., viz., E. ... more Hydrodistillation of the dried leaves of five species of the genus Eucalyptus L He¤ r., viz., E. dundasii Maiden, E. globulus Labill., E. kitsoniana Maiden, E. leucoxylon F. Muell., and E. populifolia Hook., harvested from Jbel Abderrahman arboreta (region of Nabeul, northeast of Tunisia) in April 2006, afforded essential oils in yields varying from 0.9 AE 0.3 to 3.8 AE 0.6%, dependent on the species. E. globulus and E. Kitsoniana provided the highest and the lowest percentage of essential oil amongst the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 127 compounds, representing 93.8 to 98.7% of the total oil composition. The contents of the different samples varied according to the species. The main components were 1,8-cineole (2; 4.7-59.2%), followed by a-pinene (1; 1.9-23.6%), trans-pinocarveol (6; 3.5-21.6%), globulol (8; 4.3-12.8%), p-cymene (3; 0.5-6.7%), aterpineol (1.5-4.5%), borneol (0.2-4.4%), pinocarvone (1.1-3.8%), aromadendrene (1.4-3.4%), isospathulenol (0.0-1.9%), fenchol (4; 0.1-2.5%), limonene (1.0-2.4%), epiglobulol (0.6-2.1%), viridiflorol (9; 0.8-1.8%), and spathulenol (0.1-1.6%). E. leucoxylon was the richest species in 2. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) separated the five Eucalyptus leaf essential oils into four groups, each constituting a chemotype.
Leaves of seven species of the genus Eucalyptus L&amp... more Leaves of seven species of the genus Eucalyptus L'Hér., viz., E. cladocalyx F. Muell., E. citriodora Hook., E. diversicolor F. Muell., E. fasciculosa F. Muell., E. grandis W. Hill, E. ovata Labill., and E. botryoides Sm., were harvested from Zerniza arboreta (region of Sejnene, northwest of Tunisia) in June 2007. Of the latter species, leaves were collected from trees having two origins, Morocco and Italy. Hydrodistillation of the dried leaves provided essential oils in yields varying from 0.4±0.0 to 3.3±0.1%, according to the species. E. citriodora had the highest mean percentage of essential oil amongst the species examined, whereas the lowest one was obtained for E. botryoides originating from Morocco. Analysis by GC (RI) and GC/MS allowed the identification of 140 compounds, representing 92.5 to 99.4% of the total oil composition. The contents of the different samples varied according to the species. The main components were 1,8-cineole (2), followed by α-pinene (1), p-cymene, borneol, α-terpineol, cryptone, spathulenol, trans-pinocarveol (4), bicyclogermacrene (5), caryophyllene oxide, and β-phellandrene. Principal components analysis and hierarchical cluster analysis separated the eight Eucalyptus leaf essential oils into five groups, each constituting a chemotype.
AE 0.2 to 3.9 AE 0.4%, dependent on the species. E. cinerea and E. exerta provided the highest an... more AE 0.2 to 3.9 AE 0.4%, dependent on the species. E. cinerea and E. exerta provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 142 components, representing 81.5 to 98.9% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8-cineole (1), followed by cryptone, spathulenol (4), p-cymene (2), viridiflorol (6), globulol (7), b-eudesmol, a-terpineol (5), limonene (8), d-piperitone, a-pinene (3), cuminal, and g-eudesmol. The principal component and the hierarchical cluster analyses separated the 13 Eucalyptus leaf essential oils into three groups, each constituting a chemotype.
The essential‐oil composition of 60 individual trees of Juniperus phoenicea L. from four Tunisian... more The essential‐oil composition of 60 individual trees of Juniperus phoenicea L. from four Tunisian populations in three different periods were investigated by GC and GC/MS analyses. 59 Compounds were identified in the oils, and a relatively high variation in their contents was found. All the oils were dominated by the terpenic hydrocarbon fraction, and the main component was α‐pinene (20.28–40.86%). The results of the oil compositions were processed by hierarchical clustering and principal component analysis (PCA) allowing establishing four groups of essential‐oils differentiated by one compound or more. Pattern of geographic variation in essential‐oil composition indicated that individuals from the continental site (Makthar) were clearly distinguished from those from littoral localities (Tabarka, Hawaria, and Rimel).
The essential oil composition of leaves of 60 individual trees of Juniperus oxycedrus L. growing ... more The essential oil composition of leaves of 60 individual trees of Juniperus oxycedrus L. growing in four locations in Tunisia harvested in three different seasons were investigated by GC and GC/MS. Seventy compounds were identified in the oils, and a relatively high variation in their contents were found. All the oils were dominated by terpenic hydrocarbons, with α‐pinene (27.35–58.03%) as the main component, followed by geranyl acetone (13; 1.96–7.14%), 13‐epimanoyl oxide (16; 1.35–6.95%), and eudesma‐4(15),7‐dien‐1‐ol (11; 1.39–4.18%). The 18 major oil components were processed by hierarchical clustering and principal component analysis (PCA) allowing to establish four groups, one divided into two subgroups, of populations according to the location and season of harvest. Their oils were differentiated by one compound or more, showing a clear seasonal and geographical polymorphism in their chemical composition allowing the identification of specific chemotypes. The pattern of geogr...
En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. ... more En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. La plante est connue pour son pouvoir antioxydant. Ce pouvoir est evalue par le dosage de l'acide carnosique, principale molecule antioxydante et precurseur de l'acide rosmarinique. Nous presentons les travaux realises sur 30 echantillons constitues par les parties aeriennes de la plante (feuilles, fleurs et jeunes pousses) et preleves dans 10 populations appartenant a trois etages bioclimatiques differents (le semi-aride superieur, le semi-aride inferieur et l'aride superieur). La determination de la teneur en acide carnosique par CLHP a montre des variations considerables selon l'origine geographique et la partie de la plante. Tenant compte de l'importance des extraits antioxydants dans la lutte contre les radicaux libres, l'evaluation du pouvoir antioxydant de romarin de Tunisie a permis de selectionner la partie utilisee d'une des dix populations etudiees presentant le meilleur profil antioxydant.
En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. ... more En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. La plante est connue pour son pouvoir antioxydant. Ce pouvoir est evalue par le dosage de l'acide carnosique, principale molecule antioxydante et precurseur de l'acide rosmarinique. Nous presentons les travaux realises sur 30 echantillons constitues par les parties aeriennes de la plante (feuilles, fleurs et jeunes pousses) et preleves dans 10 populations appartenant a trois etages bioclimatiques differents (le semi-aride superieur, le semi-aride inferieur et l'aride superieur). La determination de la teneur en acide carnosique par CLHP a montre des variations considerables selon l'origine geographique et la partie de la plante. Tenant compte de l'importance des extraits antioxydants dans la lutte contre les radicaux libres, l'evaluation du pouvoir antioxydant de romarin de Tunisie a permis de selectionner la partie utilisee d'une des dix populations etudiees presentant le meilleur profil antioxydant.
Journal of Experimental Biology and Agricultural Sciences, 2016
The intra-and inter populational geographic variation of eight distant populations of Juniperus p... more The intra-and inter populational geographic variation of eight distant populations of Juniperus phoenicea L. originating from Tunisia was examined biometrically on the basis of morphological-key characters of branches and cones. Seven and eight qualitative and quantitative (measured) traits of each branches and cones were studied on 45 and 29 vouchers, respectively. The principal component analysis (PCA) and hierarchical cluster analysis (HCA) of euclidean distances were performed to verify the hypothesis of geographic differentiation and for the existence of two J. phoenicea L. subspecies. The sampled populations differed significantly from one another, while the intra-populational variation was lower. Two major groups emerged on the PCA and HCA results, separating the continental populations from the coastal ones. Compared to the morphological traits of J. phoenicea subspecies in the literature and the basic flora, we confirm the existence of two J. phoenicea taxa: J. phoenicea ssp. turbinata was the reported from the coastal sites and J. phoenicea ssp. phoenicea located in the continental ones.
This study is designed to examine the chemical composition and antioxidant activity of the essent... more This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50–99.57% of the oils. Major components of the oils were α-pinene (58.61–77.39%), camphene (0.67–9.31%), δ-3-carene (0–10.01%) and trans-verbenol (0–5.24%). Antioxidant activities were determined
Essential oils fromJuniperus oxycedrusL. have been used since antiquity for fragrance, flavoring,... more Essential oils fromJuniperus oxycedrusL. have been used since antiquity for fragrance, flavoring, medicinal, antimicrobial, insecticidal, and cosmetic purposes. Several works studied the chemical composition of the essential oils ofJuniperus oxycedrusleaves. The aim of this study is to investigate the chemotaxonomic relationships and antibacterial activity of two Tunisian subspecies:Juniperus oxycedrusssp.oxycedrus(L. K.) Deb. andJuniperus oxycedrusssp.macrocarpa(S. & m.) Ball. In addition, and for the first time, we reported the antibacterial activities of TunisianJ. oxycedrusssp.macrocarpaandJ. oxycedrusssp.oxycedrusagainst four bacteria. Essential oils obtained by hydrodistillation were analysed by GC and GC/MS. Fifty-five constituents were identified. Thirty four major compounds were retained for the study of the chemical variability, andα-pinene, sylvestrene,p-cymene, and 13-epi-manoyl oxide were the main ones. The chemical principal components analysis (PCA) identified three c...
ABSTRACT Isolation of essential oil from dried leaves of Juniperus phoenicea of Tunisia were obta... more ABSTRACT Isolation of essential oil from dried leaves of Juniperus phoenicea of Tunisia were obtained with two different methods: by means of the classical hydrodistillation (HD) and by using carbon dioxide in the supercritical state (SFE). Operative conditions adopted in SFE were: pressure, 90 bar and temperature, 50 °C. The leaf volatile oil was made up of germacrene D (12.6 %), (E)-caryophyllene (7.2 %), cubebol (6.5 %), ô-cadinene (6.0 %) and Ot-terpinyl acetate (5.6 %). The corresponding yield (w/w) was 0.64 %. The essential oil obtained by hydrodistillation at a yield of (0.26 %) was characterized by Ot-pinene (16.4 %), ô-cadinene (8.1 %) germacrene D (8.0 %), a-terpinyl acetate (7.1 %) and (E)-caryophyllene (5.8 %) as main constituents.
Hydrodistillation of the dried leaves of eleven species of the genus Eucalyptus L'Hér., i.e.,... more Hydrodistillation of the dried leaves of eleven species of the genus Eucalyptus L'Hér., i.e., E. astringens Maiden, E. camaldulensis Dehnh., E. diversifolia Bonpl., E. falcata Turcz., E. ficifolia F. Muell., E. gomphocephala DC., E. lehmannii (Schauer) Benth., E. maculata Hook., E. platypus Hook., E. polyanthemos Schauer, and E. rudis Endl., harvested from Korbous arboreta (region of Nabeul, northeast of Tunisia) in April 2006, afforded essential oils in yields varying from 0.1±0.1 to 3.8±0.1%, dependent on the species. E. astringens and E. ficifolia showed the highest and the lowest mean percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 138 components, representing 74.0 to 99.1% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8‐cineole, followed by trans‐pinocarveol (1), spathulenol (2), α‐pinene, p‐cymene, (E,E)‐farnesol, cry...
Hydrodistillation of the dried leaves of 13 species of the genus Eucalyptus L&amp... more Hydrodistillation of the dried leaves of 13 species of the genus Eucalyptus L' Hér., viz., E. bicostata Maiden, Blakely & Simmonds, E. cinerea F. Muell. ex Benth., E. exerta F. Muell., E. gigantea Hook. f., E. gunnii Hook. f., E. macarthurii Deane & Maiden., E. macrorrhyncha F. Muell., E. maidenii F. Muell., E. odorata Behr., E. pauciflora Sieber ex Sprengel, E. sideroxylon A. Cunn. ex Woolls, E. tereticornis Sm., and E. viminalis Labill., harvested from Souinet arboreta (region of Ain Draaham, north of Tunisia) in June 2006, afforded essential oils in yields varying from 0.5+/-0.2 to 3.9+/-0.4%, dependent on the species. E. cinerea and E. exerta provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 142 components, representing 81.5 to 98.9% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8-cineole (1), followed by cryptone, spathulenol (4), p-cymene (2), viridiflorol (6), globulol (7), beta-eudesmol, alpha-terpineol (5), limonene (8), D-piperitone, alpha-pinene (3), cuminal, and gamma-eudesmol. The principal component and the hierarchical cluster analyses separated the 13 Eucalyptus leaf essential oils into three groups, each constituting a chemotype.
This study is designed to examine the chemical composition and antioxidant activity of the essent... more This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50-99.57% of the oils. Major components of the oils were α-pinene (58.61-77.39%), camphene (0.67-9.31%), δ-3-carene (0-10.01%) and trans-verbenol (0-5.24%). Antioxidant activities were determined by two different test systems, DPPH and ABTS radical scavenging activities. In both systems ripe berries exhibited better activity potential than the unripe ones.
Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e.... more Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e., E. brockwayi C. A. Gardn., E. gracilis F. Muell., E. gillii Maiden, E. largiflorens F. Muell., E. loxophleba Benth., E. occidentalis Endl., E. oldfieldii F. Muell., E. salmonophloia F. Muell., E. sargentii Maiden, E. stricklandii Maiden, E. torquata Luehm., and E. woodwardii Maiden, harvested from Hajeb Layoun arboreta (region of Kairouan, central Tunisia) in January 2005, afforded essential oils in yields varying from 0.5±0.1 to 5.7±0.5%, dependent on the species. E. sargentii and E. brockwayi provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 133 components, representing 92.9–98.8% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8‐cineole, terpinen‐4‐ol, α‐pinene (2), p‐cymene, aromadendrene (1), globulol (5), trans‐pinocarveol (6), spathulenol (7), β‐eudesmol, torquatone (3), and 4‐methylpentan‐2‐yl acetate (8). The principal component analysis and the hierarchical clustering indicated that the volatile leaf oil composition of the twelve Eucalyptus species could be clearly differentiated.
Stems, leaves, buds and flowers of Laurus nobilis L. growing wild in Tunisia were analysed for th... more Stems, leaves, buds and flowers of Laurus nobilis L. growing wild in Tunisia were analysed for their essential oil composition. The essential oil of Laurus nobilis L. gathered from different stations were isolated by hydrodistillation and analysed by GC/MS. The oil yields on a dry weight basis ranged between 0.4% and 1.1%. The major component identified was 1,8-cineole, other predominant components were alpha-terpinyl acetate, methyl eugenol, eugenol and linalool. Although the same compounds were present in all plant organs, the leaves differed from the stems in the concentration of 1,8-cineole and methyl eugenol, buds and flowers in the concentration of 1,8-cineole and the stem's oil composition differs from the others in content of methyl eugenol. The results obtained from GC/MS analysis of the volatile oils from individual plant organs were submitted to principal component analysis. Chemometric investigations led to differentiation of stems, leaves and buds-flowers with the respect to the content of 1,8-cineole, metyhyl eugenol and alpha-terpynil acetate; flowers and buds were non-differentiated. Finally, the antibacterial activity of the leaves' essential oils has been assayed.
Present study has been carried out to estimate the impact of geographical distribution on the yie... more Present study has been carried out to estimate the impact of geographical distribution on the yield and chemical constitute of three Eucalyptus verities viz E. cinerea F. Muell. ex Benth., E. astringens Maiden and E. sideroxylon A.Cunn. ex Schauer-. These species were collected from six arboreta of Tunisia in January 2008. The essential oil was extracted by hydrodistillation method and estimated the essential oil yield which varies from 1.5±0.1% to 4.0±0.2%. Results of the study revealed that yield of essential oil are not only depends on the Eucalyptus species but also depends on the origin of harvest. E. sideroxylon A. Cunn. exWoolls, cultivated in jbel abderrahman arboreta and E. cinerea F. Muell. ex Benth. from choucha (sejnanae) arboreta provided the lowest and the highest percentage of essential oil amongst all the studied provenances, respectively. GC (RI) and GC/MS analysis showed the presence of 163 components, representing 98.8 to 99.5% of the total oil. The contents of th...
This study is outlined to probe the chemical composition of essential oil and in vitro antioxidan... more This study is outlined to probe the chemical composition of essential oil and in vitro antioxidant activity of Juniperus oxycedrus ssp. macrocarpa (S. & m.) Ball. and Juniperus oxycedrus L. ssp. rufescens (L. K.) berries, collected from four sites, according to their maturity phase. The chemical composition of the hydrodistilled essential oil was analysed by GC-MS. Forty-eight compounds were identified, accounting for approximately 79.8-98.9% of the oil. The main constituents were α-pinene, germacrene D, myrcene, abietadiene and cis-calamenene, their mean percentage vary according to their phenological stage. The antioxidant activity of the samples was determined by the ABTS and DPPH radical scavenging activities. Hawaria essential oil extracted from mature berries showed the highest antioxidant capacity.
Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e.... more Hydrodistillation of the dried leaves of twelve species of the genus Eucalyptus L' Hér., i.e., E. brockwayi C. A. Gardn., E. gracilis F. Muell., E. gillii Maiden, E. largiflorens F. Muell., E. loxophleba Benth., E. occidentalis Endl., E. oldfieldii F. Muell., E. salmonophloia F. Muell., E. sargentii Maiden, E. stricklandii Maiden, E. torquata Luehm., and E. woodwardii Maiden, harvested from Hajeb Layoun arboreta (region of Kairouan, central Tunisia) in January 2005, afforded essential oils in yields varying from 0.5±0.1 to 5.7±0.5%, dependent on the species. E. sargentii and E. brockwayi provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 133 components, representing 92.9–98.8% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8‐cineole, terpinen‐4‐ol, α‐pinene (2), p‐cymene, aromadendrene (1), g...
Hydrodistillation of the dried leaves of five species of the genus Eucalyptus L He¤ r., viz., E. ... more Hydrodistillation of the dried leaves of five species of the genus Eucalyptus L He¤ r., viz., E. dundasii Maiden, E. globulus Labill., E. kitsoniana Maiden, E. leucoxylon F. Muell., and E. populifolia Hook., harvested from Jbel Abderrahman arboreta (region of Nabeul, northeast of Tunisia) in April 2006, afforded essential oils in yields varying from 0.9 AE 0.3 to 3.8 AE 0.6%, dependent on the species. E. globulus and E. Kitsoniana provided the highest and the lowest percentage of essential oil amongst the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 127 compounds, representing 93.8 to 98.7% of the total oil composition. The contents of the different samples varied according to the species. The main components were 1,8-cineole (2; 4.7-59.2%), followed by a-pinene (1; 1.9-23.6%), trans-pinocarveol (6; 3.5-21.6%), globulol (8; 4.3-12.8%), p-cymene (3; 0.5-6.7%), aterpineol (1.5-4.5%), borneol (0.2-4.4%), pinocarvone (1.1-3.8%), aromadendrene (1.4-3.4%), isospathulenol (0.0-1.9%), fenchol (4; 0.1-2.5%), limonene (1.0-2.4%), epiglobulol (0.6-2.1%), viridiflorol (9; 0.8-1.8%), and spathulenol (0.1-1.6%). E. leucoxylon was the richest species in 2. Principal component analysis (PCA) and hierarchical cluster analysis (HCA) separated the five Eucalyptus leaf essential oils into four groups, each constituting a chemotype.
Leaves of seven species of the genus Eucalyptus L&amp... more Leaves of seven species of the genus Eucalyptus L'Hér., viz., E. cladocalyx F. Muell., E. citriodora Hook., E. diversicolor F. Muell., E. fasciculosa F. Muell., E. grandis W. Hill, E. ovata Labill., and E. botryoides Sm., were harvested from Zerniza arboreta (region of Sejnene, northwest of Tunisia) in June 2007. Of the latter species, leaves were collected from trees having two origins, Morocco and Italy. Hydrodistillation of the dried leaves provided essential oils in yields varying from 0.4±0.0 to 3.3±0.1%, according to the species. E. citriodora had the highest mean percentage of essential oil amongst the species examined, whereas the lowest one was obtained for E. botryoides originating from Morocco. Analysis by GC (RI) and GC/MS allowed the identification of 140 compounds, representing 92.5 to 99.4% of the total oil composition. The contents of the different samples varied according to the species. The main components were 1,8-cineole (2), followed by α-pinene (1), p-cymene, borneol, α-terpineol, cryptone, spathulenol, trans-pinocarveol (4), bicyclogermacrene (5), caryophyllene oxide, and β-phellandrene. Principal components analysis and hierarchical cluster analysis separated the eight Eucalyptus leaf essential oils into five groups, each constituting a chemotype.
AE 0.2 to 3.9 AE 0.4%, dependent on the species. E. cinerea and E. exerta provided the highest an... more AE 0.2 to 3.9 AE 0.4%, dependent on the species. E. cinerea and E. exerta provided the highest and the lowest percentage of essential oil amongst all the species examined, respectively. Analysis by GC (RI) and GC/MS allowed the identification of 142 components, representing 81.5 to 98.9% of the total oil. The contents of the different samples varied according to the species. The main components were 1,8-cineole (1), followed by cryptone, spathulenol (4), p-cymene (2), viridiflorol (6), globulol (7), b-eudesmol, a-terpineol (5), limonene (8), d-piperitone, a-pinene (3), cuminal, and g-eudesmol. The principal component and the hierarchical cluster analyses separated the 13 Eucalyptus leaf essential oils into three groups, each constituting a chemotype.
The essential‐oil composition of 60 individual trees of Juniperus phoenicea L. from four Tunisian... more The essential‐oil composition of 60 individual trees of Juniperus phoenicea L. from four Tunisian populations in three different periods were investigated by GC and GC/MS analyses. 59 Compounds were identified in the oils, and a relatively high variation in their contents was found. All the oils were dominated by the terpenic hydrocarbon fraction, and the main component was α‐pinene (20.28–40.86%). The results of the oil compositions were processed by hierarchical clustering and principal component analysis (PCA) allowing establishing four groups of essential‐oils differentiated by one compound or more. Pattern of geographic variation in essential‐oil composition indicated that individuals from the continental site (Makthar) were clearly distinguished from those from littoral localities (Tabarka, Hawaria, and Rimel).
The essential oil composition of leaves of 60 individual trees of Juniperus oxycedrus L. growing ... more The essential oil composition of leaves of 60 individual trees of Juniperus oxycedrus L. growing in four locations in Tunisia harvested in three different seasons were investigated by GC and GC/MS. Seventy compounds were identified in the oils, and a relatively high variation in their contents were found. All the oils were dominated by terpenic hydrocarbons, with α‐pinene (27.35–58.03%) as the main component, followed by geranyl acetone (13; 1.96–7.14%), 13‐epimanoyl oxide (16; 1.35–6.95%), and eudesma‐4(15),7‐dien‐1‐ol (11; 1.39–4.18%). The 18 major oil components were processed by hierarchical clustering and principal component analysis (PCA) allowing to establish four groups, one divided into two subgroups, of populations according to the location and season of harvest. Their oils were differentiated by one compound or more, showing a clear seasonal and geographical polymorphism in their chemical composition allowing the identification of specific chemotypes. The pattern of geogr...
En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. ... more En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. La plante est connue pour son pouvoir antioxydant. Ce pouvoir est evalue par le dosage de l'acide carnosique, principale molecule antioxydante et precurseur de l'acide rosmarinique. Nous presentons les travaux realises sur 30 echantillons constitues par les parties aeriennes de la plante (feuilles, fleurs et jeunes pousses) et preleves dans 10 populations appartenant a trois etages bioclimatiques differents (le semi-aride superieur, le semi-aride inferieur et l'aride superieur). La determination de la teneur en acide carnosique par CLHP a montre des variations considerables selon l'origine geographique et la partie de la plante. Tenant compte de l'importance des extraits antioxydants dans la lutte contre les radicaux libres, l'evaluation du pouvoir antioxydant de romarin de Tunisie a permis de selectionner la partie utilisee d'une des dix populations etudiees presentant le meilleur profil antioxydant.
En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. ... more En Tunisie, les nappes de romarin {Rosmarinus officinalis L.) couvrent plus de 350 000 hectares. La plante est connue pour son pouvoir antioxydant. Ce pouvoir est evalue par le dosage de l'acide carnosique, principale molecule antioxydante et precurseur de l'acide rosmarinique. Nous presentons les travaux realises sur 30 echantillons constitues par les parties aeriennes de la plante (feuilles, fleurs et jeunes pousses) et preleves dans 10 populations appartenant a trois etages bioclimatiques differents (le semi-aride superieur, le semi-aride inferieur et l'aride superieur). La determination de la teneur en acide carnosique par CLHP a montre des variations considerables selon l'origine geographique et la partie de la plante. Tenant compte de l'importance des extraits antioxydants dans la lutte contre les radicaux libres, l'evaluation du pouvoir antioxydant de romarin de Tunisie a permis de selectionner la partie utilisee d'une des dix populations etudiees presentant le meilleur profil antioxydant.
Journal of Experimental Biology and Agricultural Sciences, 2016
The intra-and inter populational geographic variation of eight distant populations of Juniperus p... more The intra-and inter populational geographic variation of eight distant populations of Juniperus phoenicea L. originating from Tunisia was examined biometrically on the basis of morphological-key characters of branches and cones. Seven and eight qualitative and quantitative (measured) traits of each branches and cones were studied on 45 and 29 vouchers, respectively. The principal component analysis (PCA) and hierarchical cluster analysis (HCA) of euclidean distances were performed to verify the hypothesis of geographic differentiation and for the existence of two J. phoenicea L. subspecies. The sampled populations differed significantly from one another, while the intra-populational variation was lower. Two major groups emerged on the PCA and HCA results, separating the continental populations from the coastal ones. Compared to the morphological traits of J. phoenicea subspecies in the literature and the basic flora, we confirm the existence of two J. phoenicea taxa: J. phoenicea ssp. turbinata was the reported from the coastal sites and J. phoenicea ssp. phoenicea located in the continental ones.
This study is designed to examine the chemical composition and antioxidant activity of the essent... more This study is designed to examine the chemical composition and antioxidant activity of the essential oil of Juniperus phoenicea L. ripe and unripe berries. GC and GC/MS analyses resulted in the detection of 42 components representing approximately 96.50–99.57% of the oils. Major components of the oils were α-pinene (58.61–77.39%), camphene (0.67–9.31%), δ-3-carene (0–10.01%) and trans-verbenol (0–5.24%). Antioxidant activities were determined
Essential oils fromJuniperus oxycedrusL. have been used since antiquity for fragrance, flavoring,... more Essential oils fromJuniperus oxycedrusL. have been used since antiquity for fragrance, flavoring, medicinal, antimicrobial, insecticidal, and cosmetic purposes. Several works studied the chemical composition of the essential oils ofJuniperus oxycedrusleaves. The aim of this study is to investigate the chemotaxonomic relationships and antibacterial activity of two Tunisian subspecies:Juniperus oxycedrusssp.oxycedrus(L. K.) Deb. andJuniperus oxycedrusssp.macrocarpa(S. & m.) Ball. In addition, and for the first time, we reported the antibacterial activities of TunisianJ. oxycedrusssp.macrocarpaandJ. oxycedrusssp.oxycedrusagainst four bacteria. Essential oils obtained by hydrodistillation were analysed by GC and GC/MS. Fifty-five constituents were identified. Thirty four major compounds were retained for the study of the chemical variability, andα-pinene, sylvestrene,p-cymene, and 13-epi-manoyl oxide were the main ones. The chemical principal components analysis (PCA) identified three c...
ABSTRACT Isolation of essential oil from dried leaves of Juniperus phoenicea of Tunisia were obta... more ABSTRACT Isolation of essential oil from dried leaves of Juniperus phoenicea of Tunisia were obtained with two different methods: by means of the classical hydrodistillation (HD) and by using carbon dioxide in the supercritical state (SFE). Operative conditions adopted in SFE were: pressure, 90 bar and temperature, 50 °C. The leaf volatile oil was made up of germacrene D (12.6 %), (E)-caryophyllene (7.2 %), cubebol (6.5 %), ô-cadinene (6.0 %) and Ot-terpinyl acetate (5.6 %). The corresponding yield (w/w) was 0.64 %. The essential oil obtained by hydrodistillation at a yield of (0.26 %) was characterized by Ot-pinene (16.4 %), ô-cadinene (8.1 %) germacrene D (8.0 %), a-terpinyl acetate (7.1 %) and (E)-caryophyllene (5.8 %) as main constituents.
Uploads
Papers by Hanene medini