Indonesia is the world's largest archipelagic state. It consists of approximately 17,508 islands ... more Indonesia is the world's largest archipelagic state. It consists of approximately 17,508 islands and is divided into 34 provinces. The population in Indonesia was 237.6 million according to 2010 census data. This makes Indonesia the fourth most populous country in the world after China, India, and the United States of America. Indonesia lies along the equator ; therefore, it has a tropical climate. Furthermore, Indonesia has significant diversity of culture due to differing geographical regions, histories, and ethnic groups. This causes all sorts of problems, such as problems in transport and communications. These problems can increase the lack of oral health care awareness among the public. According to the 2013 national report on basic health research (Riset Kesehatan Dasar/RISKESDAS), 53.2% of the population−93,998,727 individuals−have active caries. In addition, dental caries tends to be more prevalent and tooth decay tends to exist to a higher degree among older individuals. The data showed that 17.6% of Indonesians aged 65 or older had lost all their teeth. This percentage is far from the WHO target of less than 5% edentulous for 2010 and indicates that oral health care awareness among the public is lacking. Disparities in the geographical distribution of dentists remain a major concern and especially affect low − income populations in rural areas. Hence, this is a significant issue affecting quality of life in Indonesia. According to the Indonesian Medical Council, there are 17,252 registered dentists in the country. In other words, only 2.4 dentists were available for every 30,000 individuals, which is far less than number that Indonesian community health centers need to provide. Therefore, to get sufficient professional and skilled dentists, the education system in dental schools needs to be reformed. In 2003, education systems in Indonesia were changing
The human oral cavity contains more than 19,000 microbial species (Keijser et al., 2008). These m... more The human oral cavity contains more than 19,000 microbial species (Keijser et al., 2008). These microorganisms interact with each other and with their host tissues (Kroes et al., 1999 ; Paster BJ et al., 2001). These complex interactions result in the formation of microbial biofilms as dental plaque and tongue surface debris, leading to caries, periodontal disease, and oral malodor. Biofilms are surface−adherent populations of microorganisms consisting of cells, water, and extra − cellular matrix material (Costerton et al. , 1995 ; Sutherland, 2001). It is now known that organisms living in biofilms behave very differently from those living in a free−floating or planktonic state (Bhardwaj et al., 2009). The ability of bacterial cells to adhere to a privileged site to build protective structures and to communicate through chemical signals enables them to counteract the host's natural defenses (Bhardwaj et al., 2009). Recent studies have suggested that several oral bacteria are opportunistic pathogens that cause systemic diseases and adverse health outcomes, such as bacterial endocarditis
The most common childhood oral infectious disease is dental caries caused by biofilm on the teeth... more The most common childhood oral infectious disease is dental caries caused by biofilm on the teeth and gums surface. It is estimated to occur five times more often than the second most common childhood illness, asthma (Peterson et al., 2013). Therefore, it is essential to understand more fully the mechanisms of cariogenic strain activity based on the biofilm formed by the microbial, which may be applied in the prevention and early diagnostics of dental caries in children. Oral biofilm have three−dimensional structures, consisting of bacterial species anchored to solid surfaces such as tooth enamel, tooth roots or dental implants (Zijnge et al., 2010). These biofilm also consist of proteins from the saliva (Huang et al., 2011). Saliva is recognized as pool of biological markers that consist of biochemical molecules, such as DNA, RNA and proteins, also the structure of microbiota (Zhang et al., 2016). Furthermore, saliva plays an important role in preventing the microbial imbalance and maintaining
International Journal of Systematic and Evolutionary Microbiology, 2021
Bacterial strain PAGU 2197T, which was isolated from soil collected from the bottom of a pond in ... more Bacterial strain PAGU 2197T, which was isolated from soil collected from the bottom of a pond in Japan, is characterized in this study. Cells of strain PAGU 2197T were aerobic, Gram-negative, short rod-shaped, non-motile, flexirubin-producing, oxidase-positive, catalase-positive and lecithinase-negative. A phylogenetic study based on 16S rRNA gene sequences and multilocus sequence analysis (gyrB, rpoB and rpoD) indicated that strain PAGU 2197T belongs to the genus Chryseobacterium and is a member of an independent lineage including Chryseobacterium tructae CCUG 60111T (sequence similarity, 95.9 %), Chryseobacterium lactis CCUG 60566T (93.4 %) and Chryseobacterium viscerum CCUG 60103T (91.6 %). The average nucleotide identity values were 80.83–85.04 %. Because average nucleotide identity values of 95–96 % exceed the 70 % DNA–DNA hybridization cutoff value for species discrimination, strain PAGU 2197T represents a novel species in the genus Chryseobacterium . The genome of strain PAGU...
Five species of oral Veillonella, V. atypica, V. denticariosi, V. dispar, V. parvula, and V. rogo... more Five species of oral Veillonella, V. atypica, V. denticariosi, V. dispar, V. parvula, and V. rogosae, have been suggested to be early colonizers of dental biofilm and causes of opportunistic infections and oral malodor. However, the pathogenicity and the distribution of oral Veillonella spp. have not been clarified. Previously, oral Veillonella spp. were identified by using 16S rDNA sequence analysis. In addition, recently, Veillonella isolates from human tongue biofilm were identified by rpoB gene sequences, but these procedures are time-consuming and complex. To overcome this problem, Igarashi et al. have designed species-specific primer sets for oral Veillonella spp. by using a highly variable region in the rpoB gene. In the present study, the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s were examined by using these species-specific primer sets. Tongue biofilms of these subjects were found to be divided into two groups based on the distribution and frequency of oral Veillonella spp. In one group, V. rogosae was the predominant species; the other group consisted of mainly V. dispar and V. atypica. Multiple factors may influence these differences in distribution and frequency of oral Veillonella spp. in tongue biofilm. This is the first report also demonstrating the availability of the species-specific primer sets for PCR to determine the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s.
Here, we report the draft genome sequence of Veillonella tobetsuensis ATCC-BAA 2400T. This bacter... more Here, we report the draft genome sequence of Veillonella tobetsuensis ATCC-BAA 2400T. This bacterium has the remarkable ability to form oral biofilms. The genome is predicted to encode the necessary enzymes involved in the pathway that facilitates the conversion of lactate to propionate.
1 Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1... more 1 Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan 2 Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Salemba Raya No.4, Jakarta Pusat 10430, Indonesia 3 Postdoctoral Fellow of Japan Society for the Promotion of Science, 5-3-1, Kouji-machi, Chiyoda-Ku, Tokyo, 102-0083, Japan 4 Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, 3435 Main Street, 109 Foster Hall, Buffalo, New York 14214, USA 5 Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Bangkok, 10400, Thailand *Corresponding author
HOPE Meeting is a special program offered by Japan Society for the Promotion of Science for excel... more HOPE Meeting is a special program offered by Japan Society for the Promotion of Science for excellent doctoral students and young researchers in the Asia-Pacific region. During the Meetings, the participants share a living space with Nobel Laureates and attend a variety of programs. The aim of this meeting is to inspire and motivate the participants to be excellent scientists. It was a very valuable experience for me to spend five days at the 5th HOPE Meeting, interacting closely with many young investigators and Nobel Laureates. My experiences at the HOPE Meeting greatly inspired my research. 受付:平成25年4月5日 受理:平成25年4月23日 The Dental Journal of Health Sciences University of Hokkaido 32 (71-74)2013 Figure 1. Participants’ nationalities (percentages) in the 5th HOPE Meeting. 71 (71) 第32巻1号 4C150 1C133/本文 ※31‐1から組体裁変更 OTF/071~074 レビュ MASHI 4C 2013.07.15 09.20.56 Page 71
Background: As the most frequent infectious disease among children worldwide, dental caries have ... more Background: As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral Veillonella are limited. The oral Veillonella community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status. Here, we studied the oral hygiene status by examining the composition and proportion of oral Veillonella species in saliva of Japanese children. Methods: Microbial samples collected from 15 Japanese children divided into three oral hygiene groups were cultured under anaerobic conditions after homogenization and dilution, and inoculated onto brain heart infusion and selective medium Veillonella agar. Genomic DNA was extracted from each isolate. Veillonella species were detected by one-step PCR using rpoB species-specific primers. To analyse the ...
The genus Veillonella is a common and abundant member of the oral microbiome. It includes eight s... more The genus Veillonella is a common and abundant member of the oral microbiome. It includes eight species, V. atypica, V. denticariosi, V. dispar, V. infantium, V. nakazawae, V. parvula, V. rogosae and V. tobetusensis. They possess important metabolic pathways that utilize lactate as an energy source. However, the overall metabolome of these species has not been studied. To further understand the metabolic framework of Veillonella in the human oral microbiome, we conducted a comparative pan-genome analysis of the eight species of oral Veillonella. Analysis of the oral Veillonella pan-genome revealed features based on KEGG pathway information to adapt to the oral environment. We found that the fructose metabolic pathway was conserved in all oral Veillonella species, and oral Veillonella have conserved pathways that utilize carbohydrates other than lactate as an energy source. This discovery may help to better understand the metabolic network among oral microbiomes and will provide guid...
We report the complete genome sequence of Veillonella nakazawae JCM 33966 T (=CCUG 74597 T ). Thi... more We report the complete genome sequence of Veillonella nakazawae JCM 33966 T (=CCUG 74597 T ). This bacterium is a member of the oral Veillonella and has the potential to be anticariogenic as an oral probiotic seed.
International Journal of Systematic and Evolutionary Microbiology
Two strains of previously unknown Gram-negative cocci, T1-7T and S6-16, were isolated from the or... more Two strains of previously unknown Gram-negative cocci, T1-7T and S6-16, were isolated from the oral cavity of healthy Japanese children. The two strains showed atypical phenotypic characteristics of members of the genus Veillonella , including catalase production. Sequencing of their 16S rRNA genes confirmed that they belong to genus Veillonella . Under anaerobic conditions, the two strains produced acetic acid and propionic acid as metabolic end-products in a trypticase–yeast extract–haemin medium containing 1 % (w/v) glucose, 1 % (w/v) fructose and 1 % (v/v) sodium lactate. Comparative analysis of the 16S rRNA, dnaK, rpoB and gltA gene sequences revealed that the two strains are phylogenetically homogeneous and comprise a distinct, novel lineage within the genus Veillonella . The sequences from the two strains shared the highest similarity, at 99.9, 95.8, 96.9 and 96.7 %, using the partial 16S rRNA, dnaK, rpoB and gltA gene sequences, respectively, with the type strains of the two...
Veillonella species are known to contribute to the formation of early oral biofilms and tend to b... more Veillonella species are known to contribute to the formation of early oral biofilms and tend to be prevalent in people with poor oral hygiene status. Here, we report the draft genome sequences of 4 oral Veillonella strains that were established recently as novel species.
Indonesia is the world's largest archipelagic state. It consists of approximately 17,508 islands ... more Indonesia is the world's largest archipelagic state. It consists of approximately 17,508 islands and is divided into 34 provinces. The population in Indonesia was 237.6 million according to 2010 census data. This makes Indonesia the fourth most populous country in the world after China, India, and the United States of America. Indonesia lies along the equator ; therefore, it has a tropical climate. Furthermore, Indonesia has significant diversity of culture due to differing geographical regions, histories, and ethnic groups. This causes all sorts of problems, such as problems in transport and communications. These problems can increase the lack of oral health care awareness among the public. According to the 2013 national report on basic health research (Riset Kesehatan Dasar/RISKESDAS), 53.2% of the population−93,998,727 individuals−have active caries. In addition, dental caries tends to be more prevalent and tooth decay tends to exist to a higher degree among older individuals. The data showed that 17.6% of Indonesians aged 65 or older had lost all their teeth. This percentage is far from the WHO target of less than 5% edentulous for 2010 and indicates that oral health care awareness among the public is lacking. Disparities in the geographical distribution of dentists remain a major concern and especially affect low − income populations in rural areas. Hence, this is a significant issue affecting quality of life in Indonesia. According to the Indonesian Medical Council, there are 17,252 registered dentists in the country. In other words, only 2.4 dentists were available for every 30,000 individuals, which is far less than number that Indonesian community health centers need to provide. Therefore, to get sufficient professional and skilled dentists, the education system in dental schools needs to be reformed. In 2003, education systems in Indonesia were changing
The human oral cavity contains more than 19,000 microbial species (Keijser et al., 2008). These m... more The human oral cavity contains more than 19,000 microbial species (Keijser et al., 2008). These microorganisms interact with each other and with their host tissues (Kroes et al., 1999 ; Paster BJ et al., 2001). These complex interactions result in the formation of microbial biofilms as dental plaque and tongue surface debris, leading to caries, periodontal disease, and oral malodor. Biofilms are surface−adherent populations of microorganisms consisting of cells, water, and extra − cellular matrix material (Costerton et al. , 1995 ; Sutherland, 2001). It is now known that organisms living in biofilms behave very differently from those living in a free−floating or planktonic state (Bhardwaj et al., 2009). The ability of bacterial cells to adhere to a privileged site to build protective structures and to communicate through chemical signals enables them to counteract the host's natural defenses (Bhardwaj et al., 2009). Recent studies have suggested that several oral bacteria are opportunistic pathogens that cause systemic diseases and adverse health outcomes, such as bacterial endocarditis
The most common childhood oral infectious disease is dental caries caused by biofilm on the teeth... more The most common childhood oral infectious disease is dental caries caused by biofilm on the teeth and gums surface. It is estimated to occur five times more often than the second most common childhood illness, asthma (Peterson et al., 2013). Therefore, it is essential to understand more fully the mechanisms of cariogenic strain activity based on the biofilm formed by the microbial, which may be applied in the prevention and early diagnostics of dental caries in children. Oral biofilm have three−dimensional structures, consisting of bacterial species anchored to solid surfaces such as tooth enamel, tooth roots or dental implants (Zijnge et al., 2010). These biofilm also consist of proteins from the saliva (Huang et al., 2011). Saliva is recognized as pool of biological markers that consist of biochemical molecules, such as DNA, RNA and proteins, also the structure of microbiota (Zhang et al., 2016). Furthermore, saliva plays an important role in preventing the microbial imbalance and maintaining
International Journal of Systematic and Evolutionary Microbiology, 2021
Bacterial strain PAGU 2197T, which was isolated from soil collected from the bottom of a pond in ... more Bacterial strain PAGU 2197T, which was isolated from soil collected from the bottom of a pond in Japan, is characterized in this study. Cells of strain PAGU 2197T were aerobic, Gram-negative, short rod-shaped, non-motile, flexirubin-producing, oxidase-positive, catalase-positive and lecithinase-negative. A phylogenetic study based on 16S rRNA gene sequences and multilocus sequence analysis (gyrB, rpoB and rpoD) indicated that strain PAGU 2197T belongs to the genus Chryseobacterium and is a member of an independent lineage including Chryseobacterium tructae CCUG 60111T (sequence similarity, 95.9 %), Chryseobacterium lactis CCUG 60566T (93.4 %) and Chryseobacterium viscerum CCUG 60103T (91.6 %). The average nucleotide identity values were 80.83–85.04 %. Because average nucleotide identity values of 95–96 % exceed the 70 % DNA–DNA hybridization cutoff value for species discrimination, strain PAGU 2197T represents a novel species in the genus Chryseobacterium . The genome of strain PAGU...
Five species of oral Veillonella, V. atypica, V. denticariosi, V. dispar, V. parvula, and V. rogo... more Five species of oral Veillonella, V. atypica, V. denticariosi, V. dispar, V. parvula, and V. rogosae, have been suggested to be early colonizers of dental biofilm and causes of opportunistic infections and oral malodor. However, the pathogenicity and the distribution of oral Veillonella spp. have not been clarified. Previously, oral Veillonella spp. were identified by using 16S rDNA sequence analysis. In addition, recently, Veillonella isolates from human tongue biofilm were identified by rpoB gene sequences, but these procedures are time-consuming and complex. To overcome this problem, Igarashi et al. have designed species-specific primer sets for oral Veillonella spp. by using a highly variable region in the rpoB gene. In the present study, the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s were examined by using these species-specific primer sets. Tongue biofilms of these subjects were found to be divided into two groups based on the distribution and frequency of oral Veillonella spp. In one group, V. rogosae was the predominant species; the other group consisted of mainly V. dispar and V. atypica. Multiple factors may influence these differences in distribution and frequency of oral Veillonella spp. in tongue biofilm. This is the first report also demonstrating the availability of the species-specific primer sets for PCR to determine the distribution and frequency of oral Veillonella spp. in the tongue biofilm of healthy adults in their 20s.
Here, we report the draft genome sequence of Veillonella tobetsuensis ATCC-BAA 2400T. This bacter... more Here, we report the draft genome sequence of Veillonella tobetsuensis ATCC-BAA 2400T. This bacterium has the remarkable ability to form oral biofilms. The genome is predicted to encode the necessary enzymes involved in the pathway that facilitates the conversion of lactate to propionate.
1 Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1... more 1 Department of Oral Microbiology, School of Dentistry, Health Sciences University of Hokkaido, 1757 Kanazawa, Ishikari-Tobetsu, Hokkaido, 061-0293, Japan 2 Department of Oral Biology, Faculty of Dentistry, Universitas Indonesia, Salemba Raya No.4, Jakarta Pusat 10430, Indonesia 3 Postdoctoral Fellow of Japan Society for the Promotion of Science, 5-3-1, Kouji-machi, Chiyoda-Ku, Tokyo, 102-0083, Japan 4 Department of Oral Biology, School of Dental Medicine, University at Buffalo, The State University of New York, 3435 Main Street, 109 Foster Hall, Buffalo, New York 14214, USA 5 Department of Oral Microbiology, Faculty of Dentistry, Mahidol University, 6 Yothi Street, Bangkok, 10400, Thailand *Corresponding author
HOPE Meeting is a special program offered by Japan Society for the Promotion of Science for excel... more HOPE Meeting is a special program offered by Japan Society for the Promotion of Science for excellent doctoral students and young researchers in the Asia-Pacific region. During the Meetings, the participants share a living space with Nobel Laureates and attend a variety of programs. The aim of this meeting is to inspire and motivate the participants to be excellent scientists. It was a very valuable experience for me to spend five days at the 5th HOPE Meeting, interacting closely with many young investigators and Nobel Laureates. My experiences at the HOPE Meeting greatly inspired my research. 受付:平成25年4月5日 受理:平成25年4月23日 The Dental Journal of Health Sciences University of Hokkaido 32 (71-74)2013 Figure 1. Participants’ nationalities (percentages) in the 5th HOPE Meeting. 71 (71) 第32巻1号 4C150 1C133/本文 ※31‐1から組体裁変更 OTF/071~074 レビュ MASHI 4C 2013.07.15 09.20.56 Page 71
Background: As the most frequent infectious disease among children worldwide, dental caries have ... more Background: As the most frequent infectious disease among children worldwide, dental caries have a strong relationship with oral hygiene status, specifically in the development of infection. However, the study regarding the identification and distribution of oral Veillonella are limited. The oral Veillonella community may affected by the differences in geographical location, age, diet, lifestyle, socio-economic status and oral hygiene status. Here, we studied the oral hygiene status by examining the composition and proportion of oral Veillonella species in saliva of Japanese children. Methods: Microbial samples collected from 15 Japanese children divided into three oral hygiene groups were cultured under anaerobic conditions after homogenization and dilution, and inoculated onto brain heart infusion and selective medium Veillonella agar. Genomic DNA was extracted from each isolate. Veillonella species were detected by one-step PCR using rpoB species-specific primers. To analyse the ...
The genus Veillonella is a common and abundant member of the oral microbiome. It includes eight s... more The genus Veillonella is a common and abundant member of the oral microbiome. It includes eight species, V. atypica, V. denticariosi, V. dispar, V. infantium, V. nakazawae, V. parvula, V. rogosae and V. tobetusensis. They possess important metabolic pathways that utilize lactate as an energy source. However, the overall metabolome of these species has not been studied. To further understand the metabolic framework of Veillonella in the human oral microbiome, we conducted a comparative pan-genome analysis of the eight species of oral Veillonella. Analysis of the oral Veillonella pan-genome revealed features based on KEGG pathway information to adapt to the oral environment. We found that the fructose metabolic pathway was conserved in all oral Veillonella species, and oral Veillonella have conserved pathways that utilize carbohydrates other than lactate as an energy source. This discovery may help to better understand the metabolic network among oral microbiomes and will provide guid...
We report the complete genome sequence of Veillonella nakazawae JCM 33966 T (=CCUG 74597 T ). Thi... more We report the complete genome sequence of Veillonella nakazawae JCM 33966 T (=CCUG 74597 T ). This bacterium is a member of the oral Veillonella and has the potential to be anticariogenic as an oral probiotic seed.
International Journal of Systematic and Evolutionary Microbiology
Two strains of previously unknown Gram-negative cocci, T1-7T and S6-16, were isolated from the or... more Two strains of previously unknown Gram-negative cocci, T1-7T and S6-16, were isolated from the oral cavity of healthy Japanese children. The two strains showed atypical phenotypic characteristics of members of the genus Veillonella , including catalase production. Sequencing of their 16S rRNA genes confirmed that they belong to genus Veillonella . Under anaerobic conditions, the two strains produced acetic acid and propionic acid as metabolic end-products in a trypticase–yeast extract–haemin medium containing 1 % (w/v) glucose, 1 % (w/v) fructose and 1 % (v/v) sodium lactate. Comparative analysis of the 16S rRNA, dnaK, rpoB and gltA gene sequences revealed that the two strains are phylogenetically homogeneous and comprise a distinct, novel lineage within the genus Veillonella . The sequences from the two strains shared the highest similarity, at 99.9, 95.8, 96.9 and 96.7 %, using the partial 16S rRNA, dnaK, rpoB and gltA gene sequences, respectively, with the type strains of the two...
Veillonella species are known to contribute to the formation of early oral biofilms and tend to b... more Veillonella species are known to contribute to the formation of early oral biofilms and tend to be prevalent in people with poor oral hygiene status. Here, we report the draft genome sequences of 4 oral Veillonella strains that were established recently as novel species.
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