Papers by Jaroslav Blaško
International Journal of Pharmaceutics
Proceedings of the National Academy of Sciences
Mycobacterium tuberculosis, one of the deadliest pathogens in human history, is distinguished by ... more Mycobacterium tuberculosis, one of the deadliest pathogens in human history, is distinguished by a unique, multilayered cell wall, which offers the bacterium a high level of protection from the attacks of the host immune system. The primary structure of the cell wall core, composed of covalently linked peptidoglycan, branched heteropolysaccharide arabinogalactan, and mycolic acids, is well known, and numerous enzymes involved in the biosynthesis of its components are characterized. The cell wall biogenesis takes place at both cytoplasmic and periplasmic faces of the plasma membrane, and only recently some of the specific transport systems translocating the metabolic intermediates between these two compartments have been characterized [M. Jackson, C. M. Stevens, L. Zhang, H. I. Zgurskaya, M. Niederweis, Chem. Rev., 10.1021/acs.chemrev.0c00869 (2020)]. In this work, we use CRISPR interference methodology in Mycobacterium smegmatis to functionally characterize an ATP-binding cassette (...
PeerJ
Thymol is a substance with a great therapeutic potential possessing antibacterial and antifungal ... more Thymol is a substance with a great therapeutic potential possessing antibacterial and antifungal activity, with a characteristic odour that remains long after application but is not pleasant at higher concentrations. In this study, attention has been focused on describing the chemical and biological properties of the simply prepared trimethylsilyl ether of thymol (kubicin). Interestingly, kubicin has similar volatility as thymol, undergoes hydrolysis in the water (moisture; forming thymol and trimethylsilanol) and can be used at 6,000 times higher concentration than thymol without any negative and irritating odour. Kubicin showed diverse fungistatic and fungicidal activities when tested by direct contact assay, or in vapour phase. The volatile vapour of kubicin was effective on all tested fungal strains. These results suggest that vapours of kubicin might provide an alternative way to fight against fungal contamination.
Science and Technology Development Journal
This paper aims to develop a non-solvent microextraction technique using INCAT (Inside Needle Cap... more This paper aims to develop a non-solvent microextraction technique using INCAT (Inside Needle Capillary Adsorption Trap) for GC-MS analysis of volatile metabolites in urine. INCATs with different sorbents were tested, resulting in an INCAT with 3 sorbent layers Chromosorb W+ 20% SE-54, Carbopack X and Carboxen 1000 (1 : 1 : 1), which proved to be the most suitable for differentiating and identifying volatile metabolites in urine samples of patients from those of healthy people.
Monatshefte für Chemie - Chemical Monthly
Acta Polytechnica
This paper investigates the Angle of Repose (AoR) of powder materials with respect to their morph... more This paper investigates the Angle of Repose (AoR) of powder materials with respect to their morphological and rheological properties. Glass beads, sand, flour and semolina of different particle sizes were used as the experimental materials. The investigated material was analysed with respect to particle shape and size. The rheological properties of the material were obtained by a shear cell test. The AoR was analysed in terms of cohesion, bulk density, particle size and circularity. More cohesive materials such as the flour samples exhibited the largest AoR > 40°, indicating their poor flowability. Glass bead samples with a high circularity value had significantly lower AoR than the flour. The Angle of Internal Friction values were not dependent on those of the AoR. Using a dimensional analysis, a mathematical model was developed to determine the AoR values based on the material properties. By the application of this model, highly accurate calculation of the value of AoR is made ...
The Journal of Physical Chemistry A
Monatshefte für Chemie - Chemical Monthly
Monatshefte für Chemie - Chemical Monthly
Particulate Science and Technology
Journal of Breath Research
Journal of separation science, Jan 30, 2017
A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino a... more A simple method for the simultaneous derivatization of carbohydrates, polyols, amines and amino acids using hexamethyldisilazane and N,O-bis(trimethylsilyl)trifluoroacetamide was developed. This method allows the direct derivatization of urine samples without sample pretreatment before derivatization. The method was successfully used for analysis of the selected metabolites in urine samples of healthy individuals and neonates suffering from galactosemia. The limits of detection by positive chemical ionization gas chromatography with tandem mass spectrometry analysis were in the range of 1.0 mgL-1 for mannitol to 4.7 mg/L for glucose.
Monatshefte für Chemie - Chemical Monthly
Monatshefte für Chemie - Chemical Monthly
Monatshefte für Chemie - Chemical Monthly, 2016
Monatshefte für Chemie - Chemical Monthly, 2016
Monatshefte für Chemie - Chemical Monthly, 2016
Journal of bacteriology, Jan 14, 2015
Mycobacterium tuberculosis possesses a thick and highly hydrophobic cell wall, composed principal... more Mycobacterium tuberculosis possesses a thick and highly hydrophobic cell wall, composed principally of a mycolic acid - arabinogalactan - peptidoglycan complex, which is critical for survival and virulence. DprE1 is a well-characterized component of decaprenyl-phospho-ribose epimerase that produces decaprenyl-phospho-arabinose (DPA) for the biosynthesis of mycobacterial arabinans. Upstream of dprE1 lies rv3789 encoding a short transmembrane protein of the GtrA family, whose members are often involved in the synthesis of cell surface polysaccharides. We demonstrate that rv3789 and dprE1 are cotranscribed from a common transcription start site situated 64 bp upstream of rv3789. Topology mapping revealed four transmembrane domains in Rv3789 and a cytoplasmic C-terminus consistent with structural models built using analysis of sequence coevolution. To investigate its role, we generated an unmarked deletion mutant of rv3789 in M. tuberculosis. The mutant was characterized by impaired gro...
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Papers by Jaroslav Blaško