Papers by Marek Kowalczyk
Croatian Medical Journal, 2015
Neurotoxicity Research, 2000
Annals of Agricultural and Environmental Medicine, 2014
Introduction and objective. Acetylcholinesterase (AChE) and cholinergic receptors play an importa... more Introduction and objective. Acetylcholinesterase (AChE) and cholinergic receptors play an important role in the immune system, including lymphocyte-induced angiogenesis. However, their exact role is not fully understood. The presented work tests the influence of isopropyl methylphosphonofluoridate (IMPF), an irreversible inhibitor of AChE, on selected immune parameters associated with angiogenesis in mice. The levels of VEGF, bFGF, TNF-α, and IFN-γ production were measured, together with the ability of lymphoid spleen cells to induce local GvH reaction after a single dose of IMPF. Materials and method. Experiments were performed in male BALB/c mice. Acetylcholinesterase activity in erythrocytes was determined by the Ellman`s procedure. Levels of cytokines were measured in serum using standard commercial ELISA kits. Influence of IMPF intoxication upon angiogenesis was examined by the LIA test, according to the Sidky and Auerbach procedure. Results. The results showed a 6-and 8-fold increase in VEGF at days 1 and 7 of the experiment, respectively, as well as a decrease (at days 14 and 21 after administration), followed by a significant increase (day 1) in bFGF levels. A statistically significant decrease in the concentration of IFN-γ was observed throughout all experiments. The maximum decrease in the level of TNF-α was found at days 1 and 7 after administration of IMPF. Additionally, a significant decrease was found in the ability to form new blood vessels following IMPF administration. Conclusions. This study revealed that IMPF has a significant effect on the regulation of lymphocyte-induced angiogenesis, which is related with the modulation of angiogenic and pro-inflammatory cytokines secretion. The observed differences suggest a possible derangement of certain elements of the neuronal and/or non-neuronal cholinergic system.
Polish journal of pharmacology and pharmacy
A new assay of afterdischarge pattern evaluation is presented, using computerized automatic analy... more A new assay of afterdischarge pattern evaluation is presented, using computerized automatic analysis where procedure of data reduction, known as syntactic analysis, was involved. Effects of phenobarbital and diazepam on afterdischarge pattern were also studied. Adopting Kalman filters technique and Markov chain midel a multi-channel analysis was performed. Significant differences between effects of two tested drugs on seizure activity propagation were found.
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Papers by Marek Kowalczyk