Rezumat Obiectiv: Investigarea influenţei microclimatului salinelor Dej şi Cacica asupra morfolog... more Rezumat Obiectiv: Investigarea influenţei microclimatului salinelor Dej şi Cacica asupra morfologiei şi exprimãrii electroforetice a fibroblastelor dermale in vitro obţinute din ţesutul hipodermic al şobolanilor Wistar, în condiţii normale şi dupã rãni şi arsuri experimentale. Materiale şi metode: culturile de fibroblaste dermale au fost obţinute din ţesutul hipodermic prelevat de la şobolani Wistar. Culturile obţinute se dezvolta în monostrat de fibroblaste ataşate la vasul de cultivare. Înainte de iniţierea culturilor, şobolanii Wistar cu greutate de 75-100 g au fost separaţi în trei loturi: control, rãni şi arsuri. Cinci animale din fiecare lot au fost trimise la salinele Dej şi Cacica timp de 14 zile şi menţinute în mediul salin, ca şi în speleoterapie. Rezultate: Speleoterapia aplicatã şobolanilor Wistar a determinat diferenţe semnificative în morfologia celularã şi în exprimarea electroforeticã a fibroblastelor dermale din culturile primare realizate. Datele obţinute confirmã ...
Rezumat Obiectiv: Investigarea influenţei microclimatului Salinei Turda asupra morfologiei şi exp... more Rezumat Obiectiv: Investigarea influenţei microclimatului Salinei Turda asupra morfologiei şi exprimãrii electroforetice a fibroblastelor pulmonare in vitro obţinute din ţesutul pulmonar al şobolanilor Wistar, în condiţii normale şi de sensibilizare cu Ovalbumina -"astmatici experimentali ". Materiale şi metode: culturile de fibroblaste pulmonare au fost obţinute din plămâni prelevaţi de la şobolani Wistar. Culturile obţinute din ţesut pulmonar de şobolan se dezvolta în monostrat de fibroblaste ataşate la vasul de cultivare. Înainte de iniţierea culturilor, şobolanii Wistar cu greutate de 75-100 g au fost separaţi în două loturi: control şi sensibilizaţi cu ovalbumină. Cinci animale din fiecare lot au fost trimise la Salina Turda timp de 14 zile şi menţinute în mediul din mina de sare, ca şi în cura speleoterapeuticã. Rezultate: Speleoterapia aplicatã şobolanilor Wistar a determinat diferenţe semnificative în morfologia celularã şi în exprimarea electroforeticã a fibroblas...
The scope of the present study was the preparation and characterization of irinotecan nanocomposi... more The scope of the present study was the preparation and characterization of irinotecan nanocomposite beads based on montmorillonite (Mt) and sodium alginate (AL) as drug carriers. After irinotecan (I) incorporation into Mt, the resulting hybrid was compounded with alginate, and I-Mt-AL nanocomposite beads were obtained by ionotropic gelation technique. The structure and surface morphology of the hybrid and composite materials were established by means of X-ray diffraction (XRD), IR spectroscopy (FT-IR), thermal analysis (TG-DTA) and scanning electron microscopy (SEM). Irinotecan incorporation efficiency in Mt and in alginate beads was determined both by UV-vis spectroscopy and thermal analysis and was found to be high. The hybrid and composite materials were tested in vitro in simulated intestinal fluid (pH 7.4, at 37 • C) in order to establish if upon administering the beads at the site of a resected colorectal tumor, the delivery of the drug is sustained and can represent an alternative to the existing systemic chemotherapy. The in vitro drug release test results clearly suggested that Mt, and Mt along with AL were able to control the release of irinotecan by making it sustained, without any burst effect, and by reducing the released amount and the release rate. The nanocomposite beads may be a promising drug delivery system in chemotherapy.
To investigate the influence of salt mine medium from the Romanian Cacica and Dej Salt Mines upon... more To investigate the influence of salt mine medium from the Romanian Cacica and Dej Salt Mines upon the cell morphology and electrophoretic expression of heterogeneous skin cell cultures obtained from Wistar rats' abdominal skin, on normal and Ovalbumin-sensitized animals.
ABSTRACT Objective: To investigate the influence of Cacica and Dej salt mines microclimate on the... more ABSTRACT Objective: To investigate the influence of Cacica and Dej salt mines microclimate on the morphology and electrophoresis protein expression of in vitro dermal fibroblasts from hypodermic tissue of Wistar rats in normal conditions and after experimental induced wounds and burns. Materials and methods: Dermal fibroblast cultures were obtained from hypodermic tissue collected from Wistar rats. Grow in cultures derived fibroblast monolayer is attached to the cultivation flask. Before initiation of cultures, Wistar rats weighing 75-100 g were separated into three groups: control, wounds and burns. Five animals from each group were sent to Cacica and Dej salt mines, for 14 days and kept in saline environment, as in speleotherapy. Results: Speleotherapy applied to Wistar rats caused significant differences in cell morphology and electrophoretic expression of dermal fibroblasts in primary culture completed. The data obtained confirm the therapeutic effects of speleotherapy compared with experimental data from control animals. Conclusions: The results of this study indicate that speleotherapy induces changes in morphology and protein expression of in vitro dermal fibroblasts and therapeutic effects of these changes are present in the wounds and burns cases.
The conference THEME: natural therapeutic factors -their role in promoting human health and survi... more The conference THEME: natural therapeutic factors -their role in promoting human health and survival of balneology resorts Organizers: National Institute of Rehabilitation, Physical Medicine and Balneoclimatology Romanian Balneology Association Locatie: Doina Complex, Neptun Theme: -Marathon of balneary resorts-Features of the medical resort bussines and the results of research activities (for this section, please register in time, limited to 12 places and get the touch with organizers, for presentation details) -Studies and researches in the filed of therapeutic mud -Sulphurous waters-mechanism of action and therapeutic effects. -Studies on micro and bioclimat. -Medical recovery in balneary resort.
Objective: To investigate the influence of salt mine medium from the Turda Salt Mine upon the cel... more Objective: To investigate the influence of salt mine medium from the Turda Salt Mine upon the cell morphology and electrophoretic expression of pulmonary fibroblasts in vitro obtained from Wistar rats' lung, in normal and Ovalbumin challenged -"asthmatic" conditions. Materials and methods: Pulmonary fibroblasts cultures were prepared from Wistar rat lung. Cultures derived from lung rat develop with a monolayer of fibroblasts attached to the culture dish. Before cultures initiation, Wistar rats of 75-100 g weight were divided in two lots: control and ovalbumin challenged animals. Five animals of each lot were send to Turda Salt Mine for 14 days and maintained in the salt mine medium, as in speleotherapy treatment.
Main therapeutic indications of salt mines and caves are represented by respiratory diseases, esp... more Main therapeutic indications of salt mines and caves are represented by respiratory diseases, especially asthma. Asthma is a disease characterized by chronic inflammation of the airways which make them hyperresponsive and change in their architecture, a process called remodeling. To solve the existing problems in allergy, pulmonology and medical recovery field and for use of natural therapeutic factors in patient treatement with different pathologies, international scientific community reviewed the therapeutic properties of caves and salt mines. Our objective was to explore the effects of speleotherapy on cellular morphology and physiology of pulmonary and dermal fibroblasts obtained from tissues of Wistar rats, in normal and Ovalbumin challenged, " asthmatic " conditions. 60 Wistar rats of 75-100 g weight were divided in two lots: control and ovalbumin challenged animals. Ten animals of each lot were send to Cacica, Turda and Dej Salt Mine for 14 days and maintained in the salt mine medium, as in speleotherapy treatment. Pulmonary and dermal fibroblasts cultures were prepared from Wistar rat lung and respectively dermal tissue. The complex picture of results was analysed and explained through biological mechanisms comparing to the control cell cultures obtained from healthy, untreated Wistar rats. In this article, we describe the supposed biological mechanisms that explain the protective effects of speleotherapy. Speleotherapy induces changes on the morphology and protein expression of pulmonary and dermal fibroblasts in vitro, and these changes-by comparing with ovalbumin sensitised animals, supports the beneficial effects of speleotherapy.
Rezumat Obiectiv: Investigarea influenţei microclimatului salinelor Dej şi Cacica asupra morfolog... more Rezumat Obiectiv: Investigarea influenţei microclimatului salinelor Dej şi Cacica asupra morfologiei şi exprimãrii electroforetice a fibroblastelor dermale in vitro obţinute din ţesutul hipodermic al şobolanilor Wistar, în condiţii normale şi dupã rãni şi arsuri experimentale. Materiale şi metode: culturile de fibroblaste dermale au fost obţinute din ţesutul hipodermic prelevat de la şobolani Wistar. Culturile obţinute se dezvolta în monostrat de fibroblaste ataşate la vasul de cultivare. Înainte de iniţierea culturilor, şobolanii Wistar cu greutate de 75-100 g au fost separaţi în trei loturi: control, rãni şi arsuri. Cinci animale din fiecare lot au fost trimise la salinele Dej şi Cacica timp de 14 zile şi menţinute în mediul salin, ca şi în speleoterapie. Rezultate: Speleoterapia aplicatã şobolanilor Wistar a determinat diferenţe semnificative în morfologia celularã şi în exprimarea electroforeticã a fibroblastelor dermale din culturile primare realizate. Datele obţinute confirmã ...
Rezumat Obiectiv: Investigarea influenţei microclimatului Salinei Turda asupra morfologiei şi exp... more Rezumat Obiectiv: Investigarea influenţei microclimatului Salinei Turda asupra morfologiei şi exprimãrii electroforetice a fibroblastelor pulmonare in vitro obţinute din ţesutul pulmonar al şobolanilor Wistar, în condiţii normale şi de sensibilizare cu Ovalbumina -"astmatici experimentali ". Materiale şi metode: culturile de fibroblaste pulmonare au fost obţinute din plămâni prelevaţi de la şobolani Wistar. Culturile obţinute din ţesut pulmonar de şobolan se dezvolta în monostrat de fibroblaste ataşate la vasul de cultivare. Înainte de iniţierea culturilor, şobolanii Wistar cu greutate de 75-100 g au fost separaţi în două loturi: control şi sensibilizaţi cu ovalbumină. Cinci animale din fiecare lot au fost trimise la Salina Turda timp de 14 zile şi menţinute în mediul din mina de sare, ca şi în cura speleoterapeuticã. Rezultate: Speleoterapia aplicatã şobolanilor Wistar a determinat diferenţe semnificative în morfologia celularã şi în exprimarea electroforeticã a fibroblas...
The scope of the present study was the preparation and characterization of irinotecan nanocomposi... more The scope of the present study was the preparation and characterization of irinotecan nanocomposite beads based on montmorillonite (Mt) and sodium alginate (AL) as drug carriers. After irinotecan (I) incorporation into Mt, the resulting hybrid was compounded with alginate, and I-Mt-AL nanocomposite beads were obtained by ionotropic gelation technique. The structure and surface morphology of the hybrid and composite materials were established by means of X-ray diffraction (XRD), IR spectroscopy (FT-IR), thermal analysis (TG-DTA) and scanning electron microscopy (SEM). Irinotecan incorporation efficiency in Mt and in alginate beads was determined both by UV-vis spectroscopy and thermal analysis and was found to be high. The hybrid and composite materials were tested in vitro in simulated intestinal fluid (pH 7.4, at 37 • C) in order to establish if upon administering the beads at the site of a resected colorectal tumor, the delivery of the drug is sustained and can represent an alternative to the existing systemic chemotherapy. The in vitro drug release test results clearly suggested that Mt, and Mt along with AL were able to control the release of irinotecan by making it sustained, without any burst effect, and by reducing the released amount and the release rate. The nanocomposite beads may be a promising drug delivery system in chemotherapy.
To investigate the influence of salt mine medium from the Romanian Cacica and Dej Salt Mines upon... more To investigate the influence of salt mine medium from the Romanian Cacica and Dej Salt Mines upon the cell morphology and electrophoretic expression of heterogeneous skin cell cultures obtained from Wistar rats' abdominal skin, on normal and Ovalbumin-sensitized animals.
ABSTRACT Objective: To investigate the influence of Cacica and Dej salt mines microclimate on the... more ABSTRACT Objective: To investigate the influence of Cacica and Dej salt mines microclimate on the morphology and electrophoresis protein expression of in vitro dermal fibroblasts from hypodermic tissue of Wistar rats in normal conditions and after experimental induced wounds and burns. Materials and methods: Dermal fibroblast cultures were obtained from hypodermic tissue collected from Wistar rats. Grow in cultures derived fibroblast monolayer is attached to the cultivation flask. Before initiation of cultures, Wistar rats weighing 75-100 g were separated into three groups: control, wounds and burns. Five animals from each group were sent to Cacica and Dej salt mines, for 14 days and kept in saline environment, as in speleotherapy. Results: Speleotherapy applied to Wistar rats caused significant differences in cell morphology and electrophoretic expression of dermal fibroblasts in primary culture completed. The data obtained confirm the therapeutic effects of speleotherapy compared with experimental data from control animals. Conclusions: The results of this study indicate that speleotherapy induces changes in morphology and protein expression of in vitro dermal fibroblasts and therapeutic effects of these changes are present in the wounds and burns cases.
The conference THEME: natural therapeutic factors -their role in promoting human health and survi... more The conference THEME: natural therapeutic factors -their role in promoting human health and survival of balneology resorts Organizers: National Institute of Rehabilitation, Physical Medicine and Balneoclimatology Romanian Balneology Association Locatie: Doina Complex, Neptun Theme: -Marathon of balneary resorts-Features of the medical resort bussines and the results of research activities (for this section, please register in time, limited to 12 places and get the touch with organizers, for presentation details) -Studies and researches in the filed of therapeutic mud -Sulphurous waters-mechanism of action and therapeutic effects. -Studies on micro and bioclimat. -Medical recovery in balneary resort.
Objective: To investigate the influence of salt mine medium from the Turda Salt Mine upon the cel... more Objective: To investigate the influence of salt mine medium from the Turda Salt Mine upon the cell morphology and electrophoretic expression of pulmonary fibroblasts in vitro obtained from Wistar rats' lung, in normal and Ovalbumin challenged -"asthmatic" conditions. Materials and methods: Pulmonary fibroblasts cultures were prepared from Wistar rat lung. Cultures derived from lung rat develop with a monolayer of fibroblasts attached to the culture dish. Before cultures initiation, Wistar rats of 75-100 g weight were divided in two lots: control and ovalbumin challenged animals. Five animals of each lot were send to Turda Salt Mine for 14 days and maintained in the salt mine medium, as in speleotherapy treatment.
Main therapeutic indications of salt mines and caves are represented by respiratory diseases, esp... more Main therapeutic indications of salt mines and caves are represented by respiratory diseases, especially asthma. Asthma is a disease characterized by chronic inflammation of the airways which make them hyperresponsive and change in their architecture, a process called remodeling. To solve the existing problems in allergy, pulmonology and medical recovery field and for use of natural therapeutic factors in patient treatement with different pathologies, international scientific community reviewed the therapeutic properties of caves and salt mines. Our objective was to explore the effects of speleotherapy on cellular morphology and physiology of pulmonary and dermal fibroblasts obtained from tissues of Wistar rats, in normal and Ovalbumin challenged, " asthmatic " conditions. 60 Wistar rats of 75-100 g weight were divided in two lots: control and ovalbumin challenged animals. Ten animals of each lot were send to Cacica, Turda and Dej Salt Mine for 14 days and maintained in the salt mine medium, as in speleotherapy treatment. Pulmonary and dermal fibroblasts cultures were prepared from Wistar rat lung and respectively dermal tissue. The complex picture of results was analysed and explained through biological mechanisms comparing to the control cell cultures obtained from healthy, untreated Wistar rats. In this article, we describe the supposed biological mechanisms that explain the protective effects of speleotherapy. Speleotherapy induces changes on the morphology and protein expression of pulmonary and dermal fibroblasts in vitro, and these changes-by comparing with ovalbumin sensitised animals, supports the beneficial effects of speleotherapy.
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