Spirulina platensis contain variety of pigments, such as chlorophylls, carotenoids, and phycocyan... more Spirulina platensis contain variety of pigments, such as chlorophylls, carotenoids, and phycocyanin. Phycocyanin (PC) exists in abundance, but due to its instability, the utilization of this pigment is still very limited. In this study, the PC was modified using formaldehyde crosslinks yielding phycocyanin-formaldehyde (PC-F), and its photostability was evaluated. The PC-F formation was designated by the distinctive alterations of the maximum absorption to 611 nm, which was 10 nm blue-shifted than those of the PC. Additionally, the sharp peaks of FTIR spectra at 1636 nm for eC]O and at 1019 nm for eCeOeC, suggesting the interaction of phycocyanin with formaldehyde. The PC-F showed stabilization improvement up to 1.53-folds after 300 mins of yellow light exposure than those of PC. Contrary to yellow light irradiation, a severe decrease of PC-F absorbance was observed reach to 4.9-folds under UV-B irradiation. The poor stability of PC-F upon white light and UV-A irradiation were indicated by the decline of PC-F absorbance up to 1.72 and 1.80, respectively. Moreover, the present study suggests that the modification of phycocyanin by formaldehyde crosslink can increase photostability upon yellow light irradiation.
ABSTRAK Sumber daya hayati biomassa mikroalga memiliki kandungan pigmen warna yang dapat dimanfaa... more ABSTRAK Sumber daya hayati biomassa mikroalga memiliki kandungan pigmen warna yang dapat dimanfaatkan sebagai antena penangkap cahaya (sensitizer), utamanya cahaya matahari. Spirulina platensis adalah salah satu sumber pigmen klorofil yang berpotensi sebagai sensitizer. Berdasarkan hasil penelitian sebelumnya, pigmen klorofil bersifat tidak stabil dan mudah rusak. Pada penelitian ini, modifikasi pigmen klorofil melalui penggantian ion logam Mg2+ sebagai atom pusat klorofil dengan ion logam Zn2+ bertujuan untuk meningkatkan kestabilan klorofil yang akan dijadikan kandidat sensitizer alami. Karakteristik pigmen klorofil, sifat stabilitas cahaya, dan stabilitas termalnya dibandingkan terhadap pigmen klorofil termodifikasi logam Zn (Zn-klorofil). Tahapan penelitian yang dilakukan yaitu ekstraksi pigmen klorofil, pembentukan turunan klorofil (feofitin), dan modifikasi klorofil dengan substitusi logam Zn. Selanjutnya, uji karakteristik tiap pigmen dilakukan menggunakan spektrofotometer UV...
Spirulina platensis contain variety of pigments, such as chlorophylls, carotenoids, and phycocyan... more Spirulina platensis contain variety of pigments, such as chlorophylls, carotenoids, and phycocyanin. Phycocyanin (PC) exists in abundance, but due to its instability, the utilization of this pigment is still very limited. In this study, the PC was modified using formaldehyde crosslinks yielding phycocyanin-formaldehyde (PC-F), and its photostability was evaluated. The PC-F formation was designated by the distinctive alterations of the maximum absorption to 611 nm, which was 10 nm blue-shifted than those of the PC. Additionally, the sharp peaks of FTIR spectra at 1636 nm for eC]O and at 1019 nm for eCeOeC, suggesting the interaction of phycocyanin with formaldehyde. The PC-F showed stabilization improvement up to 1.53-folds after 300 mins of yellow light exposure than those of PC. Contrary to yellow light irradiation, a severe decrease of PC-F absorbance was observed reach to 4.9-folds under UV-B irradiation. The poor stability of PC-F upon white light and UV-A irradiation were indicated by the decline of PC-F absorbance up to 1.72 and 1.80, respectively. Moreover, the present study suggests that the modification of phycocyanin by formaldehyde crosslink can increase photostability upon yellow light irradiation.
ABSTRAK Sumber daya hayati biomassa mikroalga memiliki kandungan pigmen warna yang dapat dimanfaa... more ABSTRAK Sumber daya hayati biomassa mikroalga memiliki kandungan pigmen warna yang dapat dimanfaatkan sebagai antena penangkap cahaya (sensitizer), utamanya cahaya matahari. Spirulina platensis adalah salah satu sumber pigmen klorofil yang berpotensi sebagai sensitizer. Berdasarkan hasil penelitian sebelumnya, pigmen klorofil bersifat tidak stabil dan mudah rusak. Pada penelitian ini, modifikasi pigmen klorofil melalui penggantian ion logam Mg2+ sebagai atom pusat klorofil dengan ion logam Zn2+ bertujuan untuk meningkatkan kestabilan klorofil yang akan dijadikan kandidat sensitizer alami. Karakteristik pigmen klorofil, sifat stabilitas cahaya, dan stabilitas termalnya dibandingkan terhadap pigmen klorofil termodifikasi logam Zn (Zn-klorofil). Tahapan penelitian yang dilakukan yaitu ekstraksi pigmen klorofil, pembentukan turunan klorofil (feofitin), dan modifikasi klorofil dengan substitusi logam Zn. Selanjutnya, uji karakteristik tiap pigmen dilakukan menggunakan spektrofotometer UV...
Uploads
Papers by Rizka Alifia