Corrosion inhibition of SAE 1018 steel by pure-culture bio®lms of Pseudomonas fragi and Escherich... more Corrosion inhibition of SAE 1018 steel by pure-culture bio®lms of Pseudomonas fragi and Escherichia coli DH5a has been evaluated in complex Luria-Bertani medium, seawater-mimicking medium, and modi®ed Baar's medium at 30°C. In batch cultures, both bacteria inhibited corrosion three to six fold compared to sterile controls, and the corrosion was comparable to that observed in anaerobic sterile media. To corroborate this result, a continuous reactor and electrochemical impedance spectroscopy were used to show that both P. fragi K and E. coli DH5a decreased the corrosion rate by 4-to 40-fold as compared to sterile controls; this matched the decrease in corrosion found with sterile medium in the absence of oxygen and with E. coli DH5a grown anaerobically. In addition, the requirement for live respiring cells was demonstrated by the increase in the corrosion rate that was observed upon killing the P. fragi K bio®lm in continuous cultures, and it was shown that fermentation products do not cause an increase in corrosion. Hence, pure-culture bio®lms inhibit corrosion of SAE 1018 steel by depleting oxygen at the metal surface.
Corrosion inhibition of SAE 1018 steel by pure-culture bio®lms of Pseudomonas fragi and Escherich... more Corrosion inhibition of SAE 1018 steel by pure-culture bio®lms of Pseudomonas fragi and Escherichia coli DH5a has been evaluated in complex Luria-Bertani medium, seawater-mimicking medium, and modi®ed Baar's medium at 30°C. In batch cultures, both bacteria inhibited corrosion three to six fold compared to sterile controls, and the corrosion was comparable to that observed in anaerobic sterile media. To corroborate this result, a continuous reactor and electrochemical impedance spectroscopy were used to show that both P. fragi K and E. coli DH5a decreased the corrosion rate by 4-to 40-fold as compared to sterile controls; this matched the decrease in corrosion found with sterile medium in the absence of oxygen and with E. coli DH5a grown anaerobically. In addition, the requirement for live respiring cells was demonstrated by the increase in the corrosion rate that was observed upon killing the P. fragi K bio®lm in continuous cultures, and it was shown that fermentation products do not cause an increase in corrosion. Hence, pure-culture bio®lms inhibit corrosion of SAE 1018 steel by depleting oxygen at the metal surface.
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