Proceedings of the National Academy of Sciences, 2009
Triple-negative breast cancers (TNBCs) are defined by a lack of expression of estrogen, progester... more Triple-negative breast cancers (TNBCs) are defined by a lack of expression of estrogen, progesterone, and HER2 receptors. Because of the absence of identified targets and targeted therapies, and due to a heterogeneous molecular presentation, treatment guidelines for patients with TNBC include only conventional chemotherapy. Such treatment, while effective for some, leaves others with high rates of early relapse and is not curative for any patient with metastatic disease. Here, we demonstrate that these tumors are sensitive to the heat shock protein 90 (Hsp90) inhibitor PU-H71. Potent and durable anti-tumor effects in TNBC xenografts, including complete response and tumor regression, without toxicity to the host are achieved with this agent. Notably, TNBC tumors respond to retreatment with PU-H71 for several cycles extending for over 5 months without evidence of resistance or toxicity. Through a proteomics approach, we show that multiple oncoproteins involved in tumor proliferation, survival, and invasive potential are in complex with PU-H71-bound Hsp90 in TNBC. PU-H71 induces efficient and sustained downregulation and inactivation, both in vitro and in vivo, of these proteins. Among them, we identify downregulation of components of the Ras/Raf/MAPK pathway and G 2-M phase to contribute to its anti-proliferative effect, degradation of activated Akt and Bcl-xL to induce apoptosis, and inhibition of activated NF-B, Akt, ERK2, Tyk2, and PKC to reduce TNBC invasive potential. The results identify Hsp90 as a critical and multimodal target in this most difficult to treat breast cancer subtype and support the use of the Hsp90 inhibitor PU-H71 for clinical trials involving patients with TNBC.
COPD (chronic obstructive pulmonary disease) is defined by a fixed expiratory airflow obstruction... more COPD (chronic obstructive pulmonary disease) is defined by a fixed expiratory airflow obstruction associated with disordered airways and alveolar destruction. COPD is caused by cigarette smoking and is the third greatest cause of mortality in the US. Forced expiratory volume in 1 second (FEV1) is the only validated clinical marker of COPD, but it correlates poorly with clinical features and is not sensitive enough to predict the early onset of disease. Using LC/MS global untargeted metabolite profiling of serum samples from a well-defined cohort of healthy smokers (n = 37), COPD smokers (n = 41) and non-smokers (n = 37), we sought to discover serum metabolic markers with known and/or unknown molecular identities that are associated with early-onset COPD. A total of 1,181 distinct molecular ions were detected in 95% of sera from all study subjects and 23 were found to be differentially-expressed in COPD-smokers vs. healthy-smokers. These 23 putative biomarkers were differentially-cor...
Archives of biochemistry and biophysics, Jan 6, 2015
Deinococcus radiodurans (Drad) is the most radioresistant organism known. Although mechanisms tha... more Deinococcus radiodurans (Drad) is the most radioresistant organism known. Although mechanisms that underlie the extreme radioresistance of Drad are incompletely defined, resistance to UV irradiation-induced killing was found to be greatly attenuated in an NO synthase (NOS) knockout strain of Drad (Δnos). We now show that endogenous NO production is also critical for protection of Drad against γ-irradiation (3000 Gy), a result of accelerated growth recovery, not protection against killing. NO-donor treatment rescued radiosensitization in Δnos Drad but did not influence radiosensitivity in wild type Drad. To discover molecular mechanisms by which endogenous NO confers radioresistance, metabolite profiling studies were performed. Untargeted LC-MS-based metabolite profiling in Drad quantified relative abundances of 1,425 molecules and levels of 294 of these were altered by >5-fold (p< 0.01). Unexpectedly, these studies identified a dramatic perturbation in carotenoid biosynthetic ...
Integrins are heterodimeric transmembrane receptors involved in sensing and transmitting informat... more Integrins are heterodimeric transmembrane receptors involved in sensing and transmitting informational cues from the extracellular environment to the cell. This study explored sub-proteome changes in response to elimination of the β3 integrin using a knockout murine model. Cleavable isotope-coded affinity tagging (cICAT) in combination with sub-cellular fractionation, multiple dimensions of separation and tandem mass spectrometry (MS/MS) were used to characterize differentially expressed proteins among β3 integrin(-/-) (β3(-/-)) mouse embryonic fibroblasts and isogenic wild-type (WT) controls. From a cytosolic protein fraction, 48 proteins were identified, in which expression differed by > 1.5-fold. Predominant ontological groups included actin-binding/cytoskeletal proteins and protease/protease inhibitors. Interestingly, β3 integrin expression was inversely correlated with expression of cathepsin B, a lysosomal cysteine protease, as its expression was greater by over 3.5-fold in...
Motor imagery electroencephalography is widely used in the brain-computer interface systems. Due ... more Motor imagery electroencephalography is widely used in the brain-computer interface systems. Due to inherent characteristics of electroencephalography signals, accurate and real-time multiclass classification is always challenging. In order to solve this problem, a multiclass posterior probability solution for twin SVM is proposed by the ranking continuous output and pairwise coupling in this paper. First, two-class posterior probability model is constructed to approximate the posterior probability by the ranking continuous output techniques and Platt's estimating method. Secondly, a solution of multiclass probabilistic outputs for twin SVM is provided by combining every pair of class probabilities according to the method of pairwise coupling. Finally, the proposed method is compared with multiclass SVM and twin SVM via voting, and multiclass posterior probability SVM using different coupling approaches. The efficacy on the classification accuracy and time complexity of the prop...
Nitration of tyrosine and other aromatic amino acid residues in proteins occurs in the setting of... more Nitration of tyrosine and other aromatic amino acid residues in proteins occurs in the setting of inflammatory, neurodegenerative, and cardiovascular diseases-importantly, this modification has been implicated in the pathogenesis of diverse diseases and the physiological process of aging. To understand the biological consequences of aromatic nitration in both health and disease, it is critical to molecularly identify the proteins that undergo nitration, specify their cognate modification sites and quantify their extent of nitration. To date, unbiased identification of nitrated proteins has often involved painstaking 2D-gel electrophoresis followed by Western Blotting with an anti-nitrotyrosine antibody for detection. Apart from being relatively slow and laborious, this method suffers from limited coverage, the potential for false-positive identifications, and failure to reveal specific amino acid modification sites. To overcome these shortcomings, we have developed a solid-phase, chemical-capture approach for unbiased and high-throughput discovery of nitrotyrosine and nitrotryptophan sites in proteins. Utilizing this method, we have successfully identified several endogenously nitrated proteins in rat brain and a total of 244 nitrated peptides from 145 proteins following in vitro exposure of rat brain homogenates to the nitrating agent peroxynitrite (1 mM). As expected, Tyr residues constituted the great majority of peroxynitrite-mediated protein nitration sites; however, we were surprised to discover several brain proteins that contain nitrated Trp residues. By incorporating a stable-isotope labeling step, this new Aromatic Nitration Site IDentification (ANSID) method was also adapted for relative quantification of nitration site abundances in proteins. Application of the ANSID method offers great potential to advance our understanding of the role of protein nitration in disease pathogenesis and normal physiology.
Proceedings of the National Academy of Sciences, 2015
Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, ... more Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, including cells of hematopoietic origin. In wild-type (WT) S49 T-lymphoma cells, signaling by cAMP and glucocorticoids converges on the induction of the proapoptotic B-cell lymphoma-family protein Bim to produce mitochondria-dependent apoptosis. Kin(-), a clonal variant of WT S49 cells, lacks PKA catalytic (PKA-Cα) activity and is resistant to cAMP-mediated apoptosis. Using sorbitol density gradient fractionation, we show here that in kin(-) S49 cells PKA-Cα is not only depleted but the residual PKA-Cα mislocalizes to heavier cell fractions and is not phosphorylated at two conserved residues (Ser(338) or Thr(197)). In WT S49 cells, PKA-regulatory subunit I (RI) and Bim coimmunoprecipitate upon treatment with cAMP analogs and forskolin (which increases endogenous cAMP concentrations). By contrast, in kin(-) cells, expression of PKA-RIα and Bim is prominently decreased, and increases in cAMP do not increase Bim expression. Even so, kin(-) cells undergo apoptosis in response to treatment with the glucocorticoid dexamethasone (Dex). In WT cells, glucorticoid-mediated apoptosis involves an increase in Bim, but in kin(-) cells, Dex-promoted cell death appears to occur by a caspase 3-independent apoptosis-inducing factor pathway. Thus, although cAMP/PKA-Cα and PKA-R1α/Bim mediate apoptotic cell death in WT S49 cells, kin(-) cells resist this response because of lower levels of PKA-Cα and PKA-RIα subunits as well as Bim. The findings for Dex-promoted apoptosis imply that these lymphoma cells have adapted to selective pressure that promotes cell death by altering canonical signaling pathways.
Proceedings of the National Academy of Sciences of the United States of America, Jan 13, 2015
Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, ... more Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, including cells of hematopoietic origin. In wild-type (WT) S49 T-lymphoma cells, signaling by cAMP and glucocorticoids converges on the induction of the proapoptotic B-cell lymphoma-family protein Bim to produce mitochondria-dependent apoptosis. Kin(-), a clonal variant of WT S49 cells, lacks PKA catalytic (PKA-Cα) activity and is resistant to cAMP-mediated apoptosis. Using sorbitol density gradient fractionation, we show here that in kin(-) S49 cells PKA-Cα is not only depleted but the residual PKA-Cα mislocalizes to heavier cell fractions and is not phosphorylated at two conserved residues (Ser(338) or Thr(197)). In WT S49 cells, PKA-regulatory subunit I (RI) and Bim coimmunoprecipitate upon treatment with cAMP analogs and forskolin (which increases endogenous cAMP concentrations). By contrast, in kin(-) cells, expression of PKA-RIα and Bim is prominently decreased, and increases in cA...
2008 11th IEEE International Conference on Communication Technology, 2008
ABSTRACT To overcome the shortcomings of information loss and reduction mistakes in traditional m... more ABSTRACT To overcome the shortcomings of information loss and reduction mistakes in traditional method of rules reduction, a new algorithm of rules reduction for real value attribute information system was proposed. Fuzzy setspsila similarity was introduced into rules reduction of information system based on rough sets theory. The corresponding condition attribute value of every rule was from 0 to 1 by evaluating every real attribute value as unitary one. Every rule was considered as a fuzzy set to denote rules similarity by fuzzy sets similarity. The rules reduction was accomplished by the improved similarity coefficient of fuzzy sets during the reduction process, and the algorithm was tested by international rice information system (IRIS) database. The experimental results show that the algorithm can correctly reduce rules reduction for real value attribute information system.
Fifth World Congress on Intelligent Control and Automation (IEEE Cat. No.04EX788), 2004
ABSTRACT It is important to evaluate the significance of system parameters in engineering, especi... more ABSTRACT It is important to evaluate the significance of system parameters in engineering, especially for reducing the dimension and complexity of system. The rough sets theory is introduced into evaluation the significance of system. A parameter defined by the concepts of lower and upper approximation in rough sets theory is used to estimate the significance of system parameters. The proposed method in the article is applied in fault analysis of diesel engine&#39;s plunger and the result matches the practical one. We can get more information of the system by this method comparing with the other methods, such as K-W method and method based on information entropy.
2006 6th World Congress on Intelligent Control and Automation, 2006
ABSTRACT The analysis and synthesis of Hinfin control via state feedback with memory for discrete... more ABSTRACT The analysis and synthesis of Hinfin control via state feedback with memory for discrete switched systems with time delay subsystems are studied. Based on multiple Lyapunov functions theory, a new sufficient condition in form of LMI is derived from the Hinfin performance analysis under arbitrary switching. Furthermore, a Hinfin controller via state feedback with memory of switched systems is established, and the result is extended to uncertain switched systems with time delay. Finally, two numerical examples show the feasibility of the designed controller
2010 The 2nd International Conference on Industrial Mechatronics and Automation, 2010
This paper describes the application of hidden Markov model (HMM) to recognition of gait mode bas... more This paper describes the application of hidden Markov model (HMM) to recognition of gait mode based on electromyographic (EMG) signals. Four types of time-domain features were extracted from the EMG signals of selected muscles within a time segment. According to the division of the gait phase, the structure of HMM was determined, in which each state is associated with a
2010 Chinese Control and Decision Conference, 2010
ABSTRACT Aiming at value reduction, a kind of RSVR algorithm was presented based on support in as... more ABSTRACT Aiming at value reduction, a kind of RSVR algorithm was presented based on support in association rules via Apriori algorithm. A more effective reduction table can be obtained by deleting those rules with less support according to least support - minsup. The reduction feasibility of this algorithm was achieved by reducing the given decision table. Testing by UCI machine learning database and comparing this algorithm with least value reduction algorithm indicate the validity of RSVR algorithm.
Zhejiang Daxue Xuebao (Gongxue Ban)/Journal of Zhejiang University (Engineering Science Edition
To overcome the shortcomings of information loss and reduction mistakes in the traditional method... more To overcome the shortcomings of information loss and reduction mistakes in the traditional method of rules reduction, a new algorithm of rules reduction for real value attribute information system is proposed. Fuzzy sets’ similarity is introduced into rules reduction of an information system based on rough sets theory. The corresponding condition attribute value of every rule was from 0 to 1 by evaluating every real attribute value as unitary one. Every rule is considered as a fuzzy set to denote rules similarity by fuzzy sets’ similarity. The rules reduction is accomplished by the improved similarity coefficient of fuzzy sets during the reduction process, and the algorithm is tested by the international rice information system (IRIS) database. The experimental results show that the algorithm can correctly reduce rules for real value attribute information system.
Twenty-seven patients with severe colon trauma treated in the Shaoxing People's Hospital from 199... more Twenty-seven patients with severe colon trauma treated in the Shaoxing People's Hospital from 1995 to 2006 were retrospectively analyzed. The patients with severe tunica muscularis injury were treated by medical glue combined with the greater omentum during operations. The initial result was encouraging. All patients were cured with no anastomotic leakage or abscess. Therefore, reasonable use of medical glue combined with the greater omentum can not only improve the curative rate but also reduces postoperative complications for patients with colon injury.
Proceedings of the National Academy of Sciences, 2009
Triple-negative breast cancers (TNBCs) are defined by a lack of expression of estrogen, progester... more Triple-negative breast cancers (TNBCs) are defined by a lack of expression of estrogen, progesterone, and HER2 receptors. Because of the absence of identified targets and targeted therapies, and due to a heterogeneous molecular presentation, treatment guidelines for patients with TNBC include only conventional chemotherapy. Such treatment, while effective for some, leaves others with high rates of early relapse and is not curative for any patient with metastatic disease. Here, we demonstrate that these tumors are sensitive to the heat shock protein 90 (Hsp90) inhibitor PU-H71. Potent and durable anti-tumor effects in TNBC xenografts, including complete response and tumor regression, without toxicity to the host are achieved with this agent. Notably, TNBC tumors respond to retreatment with PU-H71 for several cycles extending for over 5 months without evidence of resistance or toxicity. Through a proteomics approach, we show that multiple oncoproteins involved in tumor proliferation, survival, and invasive potential are in complex with PU-H71-bound Hsp90 in TNBC. PU-H71 induces efficient and sustained downregulation and inactivation, both in vitro and in vivo, of these proteins. Among them, we identify downregulation of components of the Ras/Raf/MAPK pathway and G 2-M phase to contribute to its anti-proliferative effect, degradation of activated Akt and Bcl-xL to induce apoptosis, and inhibition of activated NF-B, Akt, ERK2, Tyk2, and PKC to reduce TNBC invasive potential. The results identify Hsp90 as a critical and multimodal target in this most difficult to treat breast cancer subtype and support the use of the Hsp90 inhibitor PU-H71 for clinical trials involving patients with TNBC.
COPD (chronic obstructive pulmonary disease) is defined by a fixed expiratory airflow obstruction... more COPD (chronic obstructive pulmonary disease) is defined by a fixed expiratory airflow obstruction associated with disordered airways and alveolar destruction. COPD is caused by cigarette smoking and is the third greatest cause of mortality in the US. Forced expiratory volume in 1 second (FEV1) is the only validated clinical marker of COPD, but it correlates poorly with clinical features and is not sensitive enough to predict the early onset of disease. Using LC/MS global untargeted metabolite profiling of serum samples from a well-defined cohort of healthy smokers (n = 37), COPD smokers (n = 41) and non-smokers (n = 37), we sought to discover serum metabolic markers with known and/or unknown molecular identities that are associated with early-onset COPD. A total of 1,181 distinct molecular ions were detected in 95% of sera from all study subjects and 23 were found to be differentially-expressed in COPD-smokers vs. healthy-smokers. These 23 putative biomarkers were differentially-cor...
Archives of biochemistry and biophysics, Jan 6, 2015
Deinococcus radiodurans (Drad) is the most radioresistant organism known. Although mechanisms tha... more Deinococcus radiodurans (Drad) is the most radioresistant organism known. Although mechanisms that underlie the extreme radioresistance of Drad are incompletely defined, resistance to UV irradiation-induced killing was found to be greatly attenuated in an NO synthase (NOS) knockout strain of Drad (Δnos). We now show that endogenous NO production is also critical for protection of Drad against γ-irradiation (3000 Gy), a result of accelerated growth recovery, not protection against killing. NO-donor treatment rescued radiosensitization in Δnos Drad but did not influence radiosensitivity in wild type Drad. To discover molecular mechanisms by which endogenous NO confers radioresistance, metabolite profiling studies were performed. Untargeted LC-MS-based metabolite profiling in Drad quantified relative abundances of 1,425 molecules and levels of 294 of these were altered by >5-fold (p< 0.01). Unexpectedly, these studies identified a dramatic perturbation in carotenoid biosynthetic ...
Integrins are heterodimeric transmembrane receptors involved in sensing and transmitting informat... more Integrins are heterodimeric transmembrane receptors involved in sensing and transmitting informational cues from the extracellular environment to the cell. This study explored sub-proteome changes in response to elimination of the β3 integrin using a knockout murine model. Cleavable isotope-coded affinity tagging (cICAT) in combination with sub-cellular fractionation, multiple dimensions of separation and tandem mass spectrometry (MS/MS) were used to characterize differentially expressed proteins among β3 integrin(-/-) (β3(-/-)) mouse embryonic fibroblasts and isogenic wild-type (WT) controls. From a cytosolic protein fraction, 48 proteins were identified, in which expression differed by > 1.5-fold. Predominant ontological groups included actin-binding/cytoskeletal proteins and protease/protease inhibitors. Interestingly, β3 integrin expression was inversely correlated with expression of cathepsin B, a lysosomal cysteine protease, as its expression was greater by over 3.5-fold in...
Motor imagery electroencephalography is widely used in the brain-computer interface systems. Due ... more Motor imagery electroencephalography is widely used in the brain-computer interface systems. Due to inherent characteristics of electroencephalography signals, accurate and real-time multiclass classification is always challenging. In order to solve this problem, a multiclass posterior probability solution for twin SVM is proposed by the ranking continuous output and pairwise coupling in this paper. First, two-class posterior probability model is constructed to approximate the posterior probability by the ranking continuous output techniques and Platt's estimating method. Secondly, a solution of multiclass probabilistic outputs for twin SVM is provided by combining every pair of class probabilities according to the method of pairwise coupling. Finally, the proposed method is compared with multiclass SVM and twin SVM via voting, and multiclass posterior probability SVM using different coupling approaches. The efficacy on the classification accuracy and time complexity of the prop...
Nitration of tyrosine and other aromatic amino acid residues in proteins occurs in the setting of... more Nitration of tyrosine and other aromatic amino acid residues in proteins occurs in the setting of inflammatory, neurodegenerative, and cardiovascular diseases-importantly, this modification has been implicated in the pathogenesis of diverse diseases and the physiological process of aging. To understand the biological consequences of aromatic nitration in both health and disease, it is critical to molecularly identify the proteins that undergo nitration, specify their cognate modification sites and quantify their extent of nitration. To date, unbiased identification of nitrated proteins has often involved painstaking 2D-gel electrophoresis followed by Western Blotting with an anti-nitrotyrosine antibody for detection. Apart from being relatively slow and laborious, this method suffers from limited coverage, the potential for false-positive identifications, and failure to reveal specific amino acid modification sites. To overcome these shortcomings, we have developed a solid-phase, chemical-capture approach for unbiased and high-throughput discovery of nitrotyrosine and nitrotryptophan sites in proteins. Utilizing this method, we have successfully identified several endogenously nitrated proteins in rat brain and a total of 244 nitrated peptides from 145 proteins following in vitro exposure of rat brain homogenates to the nitrating agent peroxynitrite (1 mM). As expected, Tyr residues constituted the great majority of peroxynitrite-mediated protein nitration sites; however, we were surprised to discover several brain proteins that contain nitrated Trp residues. By incorporating a stable-isotope labeling step, this new Aromatic Nitration Site IDentification (ANSID) method was also adapted for relative quantification of nitration site abundances in proteins. Application of the ANSID method offers great potential to advance our understanding of the role of protein nitration in disease pathogenesis and normal physiology.
Proceedings of the National Academy of Sciences, 2015
Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, ... more Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, including cells of hematopoietic origin. In wild-type (WT) S49 T-lymphoma cells, signaling by cAMP and glucocorticoids converges on the induction of the proapoptotic B-cell lymphoma-family protein Bim to produce mitochondria-dependent apoptosis. Kin(-), a clonal variant of WT S49 cells, lacks PKA catalytic (PKA-Cα) activity and is resistant to cAMP-mediated apoptosis. Using sorbitol density gradient fractionation, we show here that in kin(-) S49 cells PKA-Cα is not only depleted but the residual PKA-Cα mislocalizes to heavier cell fractions and is not phosphorylated at two conserved residues (Ser(338) or Thr(197)). In WT S49 cells, PKA-regulatory subunit I (RI) and Bim coimmunoprecipitate upon treatment with cAMP analogs and forskolin (which increases endogenous cAMP concentrations). By contrast, in kin(-) cells, expression of PKA-RIα and Bim is prominently decreased, and increases in cAMP do not increase Bim expression. Even so, kin(-) cells undergo apoptosis in response to treatment with the glucocorticoid dexamethasone (Dex). In WT cells, glucorticoid-mediated apoptosis involves an increase in Bim, but in kin(-) cells, Dex-promoted cell death appears to occur by a caspase 3-independent apoptosis-inducing factor pathway. Thus, although cAMP/PKA-Cα and PKA-R1α/Bim mediate apoptotic cell death in WT S49 cells, kin(-) cells resist this response because of lower levels of PKA-Cα and PKA-RIα subunits as well as Bim. The findings for Dex-promoted apoptosis imply that these lymphoma cells have adapted to selective pressure that promotes cell death by altering canonical signaling pathways.
Proceedings of the National Academy of Sciences of the United States of America, Jan 13, 2015
Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, ... more Cyclic AMP/protein kinase A (cAMP/PKA) and glucocorticoids promote the death of many cell types, including cells of hematopoietic origin. In wild-type (WT) S49 T-lymphoma cells, signaling by cAMP and glucocorticoids converges on the induction of the proapoptotic B-cell lymphoma-family protein Bim to produce mitochondria-dependent apoptosis. Kin(-), a clonal variant of WT S49 cells, lacks PKA catalytic (PKA-Cα) activity and is resistant to cAMP-mediated apoptosis. Using sorbitol density gradient fractionation, we show here that in kin(-) S49 cells PKA-Cα is not only depleted but the residual PKA-Cα mislocalizes to heavier cell fractions and is not phosphorylated at two conserved residues (Ser(338) or Thr(197)). In WT S49 cells, PKA-regulatory subunit I (RI) and Bim coimmunoprecipitate upon treatment with cAMP analogs and forskolin (which increases endogenous cAMP concentrations). By contrast, in kin(-) cells, expression of PKA-RIα and Bim is prominently decreased, and increases in cA...
2008 11th IEEE International Conference on Communication Technology, 2008
ABSTRACT To overcome the shortcomings of information loss and reduction mistakes in traditional m... more ABSTRACT To overcome the shortcomings of information loss and reduction mistakes in traditional method of rules reduction, a new algorithm of rules reduction for real value attribute information system was proposed. Fuzzy setspsila similarity was introduced into rules reduction of information system based on rough sets theory. The corresponding condition attribute value of every rule was from 0 to 1 by evaluating every real attribute value as unitary one. Every rule was considered as a fuzzy set to denote rules similarity by fuzzy sets similarity. The rules reduction was accomplished by the improved similarity coefficient of fuzzy sets during the reduction process, and the algorithm was tested by international rice information system (IRIS) database. The experimental results show that the algorithm can correctly reduce rules reduction for real value attribute information system.
Fifth World Congress on Intelligent Control and Automation (IEEE Cat. No.04EX788), 2004
ABSTRACT It is important to evaluate the significance of system parameters in engineering, especi... more ABSTRACT It is important to evaluate the significance of system parameters in engineering, especially for reducing the dimension and complexity of system. The rough sets theory is introduced into evaluation the significance of system. A parameter defined by the concepts of lower and upper approximation in rough sets theory is used to estimate the significance of system parameters. The proposed method in the article is applied in fault analysis of diesel engine&#39;s plunger and the result matches the practical one. We can get more information of the system by this method comparing with the other methods, such as K-W method and method based on information entropy.
2006 6th World Congress on Intelligent Control and Automation, 2006
ABSTRACT The analysis and synthesis of Hinfin control via state feedback with memory for discrete... more ABSTRACT The analysis and synthesis of Hinfin control via state feedback with memory for discrete switched systems with time delay subsystems are studied. Based on multiple Lyapunov functions theory, a new sufficient condition in form of LMI is derived from the Hinfin performance analysis under arbitrary switching. Furthermore, a Hinfin controller via state feedback with memory of switched systems is established, and the result is extended to uncertain switched systems with time delay. Finally, two numerical examples show the feasibility of the designed controller
2010 The 2nd International Conference on Industrial Mechatronics and Automation, 2010
This paper describes the application of hidden Markov model (HMM) to recognition of gait mode bas... more This paper describes the application of hidden Markov model (HMM) to recognition of gait mode based on electromyographic (EMG) signals. Four types of time-domain features were extracted from the EMG signals of selected muscles within a time segment. According to the division of the gait phase, the structure of HMM was determined, in which each state is associated with a
2010 Chinese Control and Decision Conference, 2010
ABSTRACT Aiming at value reduction, a kind of RSVR algorithm was presented based on support in as... more ABSTRACT Aiming at value reduction, a kind of RSVR algorithm was presented based on support in association rules via Apriori algorithm. A more effective reduction table can be obtained by deleting those rules with less support according to least support - minsup. The reduction feasibility of this algorithm was achieved by reducing the given decision table. Testing by UCI machine learning database and comparing this algorithm with least value reduction algorithm indicate the validity of RSVR algorithm.
Zhejiang Daxue Xuebao (Gongxue Ban)/Journal of Zhejiang University (Engineering Science Edition
To overcome the shortcomings of information loss and reduction mistakes in the traditional method... more To overcome the shortcomings of information loss and reduction mistakes in the traditional method of rules reduction, a new algorithm of rules reduction for real value attribute information system is proposed. Fuzzy sets’ similarity is introduced into rules reduction of an information system based on rough sets theory. The corresponding condition attribute value of every rule was from 0 to 1 by evaluating every real attribute value as unitary one. Every rule is considered as a fuzzy set to denote rules similarity by fuzzy sets’ similarity. The rules reduction is accomplished by the improved similarity coefficient of fuzzy sets during the reduction process, and the algorithm is tested by the international rice information system (IRIS) database. The experimental results show that the algorithm can correctly reduce rules for real value attribute information system.
Twenty-seven patients with severe colon trauma treated in the Shaoxing People's Hospital from 199... more Twenty-seven patients with severe colon trauma treated in the Shaoxing People's Hospital from 1995 to 2006 were retrospectively analyzed. The patients with severe tunica muscularis injury were treated by medical glue combined with the greater omentum during operations. The initial result was encouraging. All patients were cured with no anastomotic leakage or abscess. Therefore, reasonable use of medical glue combined with the greater omentum can not only improve the curative rate but also reduces postoperative complications for patients with colon injury.
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Papers by Yuliang Ma