Papers by Zahra Baghestani
Microscopy and Microanalysis, 2014
American journal of human genetics, Jan 9, 2015
Clustered regularly interspaced short palindromic repeat (CRISPR) has arisen as a frontrunner for... more Clustered regularly interspaced short palindromic repeat (CRISPR) has arisen as a frontrunner for efficient genome engineering. However, the potentially broad therapeutic implications are largely unexplored. Here, to investigate the therapeutic potential of CRISPR/Cas9 in a diverse set of genetic disorders, we establish a pipeline that uses readily obtainable cells from affected individuals. We show that an adapted version of CRISPR/Cas9 increases the amount of utrophin, a known disease modifier in Duchenne muscular dystrophy (DMD). Furthermore, we demonstrate preferential elimination of the dominant-negative FGFR3 c.1138G>A allele in fibroblasts of an individual affected by achondroplasia. Using a previously undescribed approach involving single guide RNA, we successfully removed large genome rearrangement in primary cells of an individual with an X chromosome duplication including MECP2. Moreover, removal of a duplication of DMD exons 18-30 in myotubes of an individual affected...
Chromosoma, 2015
Chromatin, once thought to serve only as a means to package DNA, is now recognized as a major reg... more Chromatin, once thought to serve only as a means to package DNA, is now recognized as a major regulator of gene activity. As a result of the wide range of methods used to describe the numerous levels of chromatin organization, the terminology that has emerged to describe these organizational states is often imprecise and sometimes misleading. In this review, we discuss our current understanding of chromatin architecture and propose terms to describe the various biochemical and structural states of chromatin.
MARCO symposium (Chalenges for …, 2009
... [1] Azizi, M. 2005. Bunium persicum essential oils suppressed potato sprouting in storage. Pr... more ... [1] Azizi, M. 2005. Bunium persicum essential oils suppressed potato sprouting in storage. Proceedings of the 36th International Symposium on Essential oils, 4-7 September, Budapest, Hungary. ... [10] Yosofi, M., 2007. Flora of Iran, Payam-e-nour publication, Tehran, 227p. ...
American Journal of Animal and Veterinary Sciences, 2011
Problem statement: The seeds of Nigella sativa Linn. (Ranunculaceae) known as black seed or black... more Problem statement: The seeds of Nigella sativa Linn. (Ranunculaceae) known as black seed or black cumin, are used in herbal medicine all over the world for the treatment and prevention of a number of diseases and conditions that include asthma, diarrhea and dyslipidaemia. Approach: In this study the effect of intramammary injection of Nigella Sativa Extract (NSE) in paraffin on quarter milk, quality and Somatic Cell Count (SCC) and the shedding pattern of Staphylococcus aureus were investigated. Thirty Holstein cows, naturally infected with S. aureus subclinical mastitis, were subjected to treatment with the NSE at a dose of 10 mL in paraffin (200 mg mL −1 ) per day for 3 days, or with 10 mL paraffin as control. The injection areas were checked for adverse reactions. The daily milk production was measured before and after treatment. Intramammary injection of NSE caused a remarkable healing. Quarter milk samples were collected for bacteriological analysis and Somatic Cell Counts (SCC). The bacterial count moderately decreased in the treatment group. After the end of the treatment, the numbers of S. aureus-infected quarters and milk SCC tended to decrease in the NSEtreated cows. These clarifications were significantly higher one week post-treatment than pretreatment. Similar changes were not observed in the control group. Results: The results of the present study showed that the NSE has potential as a therapeutic agent for S. aureus infection causing subclinical mastitis of dairy cows and may contribute to the cow's recovery from mastitis. Conclusion:
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Papers by Zahra Baghestani