In the present research the protective effect of green tea ethanol extract (TE) with or without m... more In the present research the protective effect of green tea ethanol extract (TE) with or without mannitol and branched chain amino acids (TAM) were evaluated in a rat model of hepatorenal syndrome (HRS). HRS was induced in rats by intraperitoneal injection of D-(+)-galactosamine hydrochloride. Control HRS rats showed significant increase in plasma bilirubin and transaminases activities with reduction in albumin compared with normal rats. Plasma creatinine, urea, endotheline-1, cholesterol, malondialdehyde, phenylalanine and tumor necrosis factor-α increased significantly with significant decrease in high density lipoprotein cholesterol, total antioxidant capacity and calcium in HRS model. Creatinine clearance was reduced while urinary N-acetyl-β-D-glucosaminidase was elevated in HRS rats. Pretreatment of rats by TE and TAM separately produced significant prevention of the induced biochemical changes by galactosamine hydrochloride. Interlukin-6 gene expression was significantly enhanced in the liver and kidney of HRS model and significantly down regulated on treatment with either TE or TAM. Liver histopathology was severely affected without any change in kidney histology in HRS rats; TAM treatment prevented the changes in liver histopathology more than TE. It can be concluded that pretreatment with TE or TAM afforded protection from HRS; TAM was more efficient.
Hepatorenal syndrome (HRS) is a major health problem where the functions of both liver and kidney... more Hepatorenal syndrome (HRS) is a major health problem where the functions of both liver and kidney are affected. The aim of the present research was to evaluate the protective effect of Nigella sativa meal and its ethanol extract in hepatorenal syndrome model in rats. Proximate composition and amino acid contents of Nigella sativa meal were analyzed. Total phenolic contents and phenolic compounds were determined in the ethanol extract. HRS was induced in rats via intraperitoneal injection of galactosamine. Three weeks prior to HRS induction, one group of rats was given daily oral dose of Nigella sativa meal alcohol extract, another group was fed on diet containing Nigella sativa meal, the third group served as control with HRS. A control normal group was run without any treatments. Different biochemical and nutritional parameters were followed. Gene expression of Interlukin 6 (IL-6) in both liver and kidney was determined. Safety of ethanol extract was assessed through acute toxicity test. Results showed that Nigella sativa meal contain 27.1% protein and a Fischer ratio of 3.303. Total phenolic content was 42.64 ± 0.929 mg gallic acid equivalent/g dry ethanol extract, identified phenolic compounds were protochatechuic, chlorogenic, vanillic, sinapic, coumaric and cinnamic acid and chrysin. HRS control group showed liver and kidney dysfunction reflected in the elevated plasma creatinine, urea, transaminases activity, total and direct bilirubin and endothelin 1 and urinary N-acetyl-β-D-Glucosaminidase together with reduction in plasma albumin and creatinine clearance. Treatment with either forms of Nigella sativa significantly improved all determined parameters except for creatinine and creatinine clearance. Reduction in hemoglobin, plasma total antioxidant capacity and calcium along with elevation of plasma malondialdehyde and tumor necrosis factor-α were noticed in HRS control. All these parameters were significantly improved on treatment with Nigella sativa meals and extract except for calcium in case of the extract. Total cholesterol and high density lipoprotein cholesterol showed dyslipidemia in HRS control which were significantly ameliorated on consumption of Nigella sativa meals and extract. Liver and kidney Interlukin 6 gene expression was significantly enhanced in HRS control and significantly down regulated on treatment with Nigella sativa meals and extract. Ethanol extract showed complete safety on the highest tested dose.
Diabetes mellitus and its complication are a worldwide health problem. Many plants have been used... more Diabetes mellitus and its complication are a worldwide health problem. Many plants have been used to improve glucose tolerance and insulin sensitivity in treatment strategies of diabetes mellitus. The present study investigated the potential therapeutic effects of herbal tea from Balanites aegyptiaca fruits on certain biochemical markers in streptozotocin-induced diabetes mellitus in rats. Diabetes was induced in rats by single intraperitoneal injection of streptozotocin (STZ) with concentration of 60 mg/kg body weight. Herbal tea solutions of B. aegyptiaca fruits with different concentration 0.25, 0.5, and 1.0% were given to diabetic rats in replacement of drinking water. Diabetic control and normal control groups were given drinking water without herbal tea. The effects of B. aegyptiaca tea on blood glucose, total cholesterol, triglyceride, total protein, urea, creatinine and the activities of liver marker enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), and antioxidants markers were examined in the plasma of control and treated rats. After four weeks of treatment with B. aegyptiaca tea; blood glucose, urea, creatinine, AST, ALT, total cholesterol and triglyceride was significantly reduced in diabetic rats. However, the levels of total protein and selected antioxidant enzymes activity were increased compared to diabetic control. Total antioxidant capacity was restored to near normal levels. The present results shown that herbal tea of B. aegyptiaca fruit has an anti-hyperglycemic effect and subsequently may improve hepatics and renal damage associated with STZ-induced diabetes mellitus in rats. Hence, the use of B. aegyptiaca fruit tea is applicable to approach health-promoting.
In the present research the protective effect of green tea ethanol extract (TE) with or without m... more In the present research the protective effect of green tea ethanol extract (TE) with or without mannitol and branched chain amino acids (TAM) were evaluated in a rat model of hepatorenal syndrome (HRS). HRS was induced in rats by intraperitoneal injection of D-(+)-galactosamine hydrochloride. Control HRS rats showed significant increase in plasma bilirubin and transaminases activities with reduction in albumin compared with normal rats. Plasma creatinine, urea, endotheline-1, cholesterol, malondialdehyde, phenylalanine and tumor necrosis factor-α increased significantly with significant decrease in high density lipoprotein cholesterol, total antioxidant capacity and calcium in HRS model. Creatinine clearance was reduced while urinary N-acetyl-β-D-glucosaminidase was elevated in HRS rats. Pretreatment of rats by TE and TAM separately produced significant prevention of the induced biochemical changes by galactosamine hydrochloride. Interlukin-6 gene expression was significantly enhanced in the liver and kidney of HRS model and significantly down regulated on treatment with either TE or TAM. Liver histopathology was severely affected without any change in kidney histology in HRS rats; TAM treatment prevented the changes in liver histopathology more than TE. It can be concluded that pretreatment with TE or TAM afforded protection from HRS; TAM was more efficient.
Hepatorenal syndrome (HRS) is a major health problem where the functions of both liver and kidney... more Hepatorenal syndrome (HRS) is a major health problem where the functions of both liver and kidney are affected. The aim of the present research was to evaluate the protective effect of Nigella sativa meal and its ethanol extract in hepatorenal syndrome model in rats. Proximate composition and amino acid contents of Nigella sativa meal were analyzed. Total phenolic contents and phenolic compounds were determined in the ethanol extract. HRS was induced in rats via intraperitoneal injection of galactosamine. Three weeks prior to HRS induction, one group of rats was given daily oral dose of Nigella sativa meal alcohol extract, another group was fed on diet containing Nigella sativa meal, the third group served as control with HRS. A control normal group was run without any treatments. Different biochemical and nutritional parameters were followed. Gene expression of Interlukin 6 (IL-6) in both liver and kidney was determined. Safety of ethanol extract was assessed through acute toxicity test. Results showed that Nigella sativa meal contain 27.1% protein and a Fischer ratio of 3.303. Total phenolic content was 42.64 ± 0.929 mg gallic acid equivalent/g dry ethanol extract, identified phenolic compounds were protochatechuic, chlorogenic, vanillic, sinapic, coumaric and cinnamic acid and chrysin. HRS control group showed liver and kidney dysfunction reflected in the elevated plasma creatinine, urea, transaminases activity, total and direct bilirubin and endothelin 1 and urinary N-acetyl-β-D-Glucosaminidase together with reduction in plasma albumin and creatinine clearance. Treatment with either forms of Nigella sativa significantly improved all determined parameters except for creatinine and creatinine clearance. Reduction in hemoglobin, plasma total antioxidant capacity and calcium along with elevation of plasma malondialdehyde and tumor necrosis factor-α were noticed in HRS control. All these parameters were significantly improved on treatment with Nigella sativa meals and extract except for calcium in case of the extract. Total cholesterol and high density lipoprotein cholesterol showed dyslipidemia in HRS control which were significantly ameliorated on consumption of Nigella sativa meals and extract. Liver and kidney Interlukin 6 gene expression was significantly enhanced in HRS control and significantly down regulated on treatment with Nigella sativa meals and extract. Ethanol extract showed complete safety on the highest tested dose.
Diabetes mellitus and its complication are a worldwide health problem. Many plants have been used... more Diabetes mellitus and its complication are a worldwide health problem. Many plants have been used to improve glucose tolerance and insulin sensitivity in treatment strategies of diabetes mellitus. The present study investigated the potential therapeutic effects of herbal tea from Balanites aegyptiaca fruits on certain biochemical markers in streptozotocin-induced diabetes mellitus in rats. Diabetes was induced in rats by single intraperitoneal injection of streptozotocin (STZ) with concentration of 60 mg/kg body weight. Herbal tea solutions of B. aegyptiaca fruits with different concentration 0.25, 0.5, and 1.0% were given to diabetic rats in replacement of drinking water. Diabetic control and normal control groups were given drinking water without herbal tea. The effects of B. aegyptiaca tea on blood glucose, total cholesterol, triglyceride, total protein, urea, creatinine and the activities of liver marker enzymes aspartate aminotransferase (AST), alanine aminotransferase (ALT), and antioxidants markers were examined in the plasma of control and treated rats. After four weeks of treatment with B. aegyptiaca tea; blood glucose, urea, creatinine, AST, ALT, total cholesterol and triglyceride was significantly reduced in diabetic rats. However, the levels of total protein and selected antioxidant enzymes activity were increased compared to diabetic control. Total antioxidant capacity was restored to near normal levels. The present results shown that herbal tea of B. aegyptiaca fruit has an anti-hyperglycemic effect and subsequently may improve hepatics and renal damage associated with STZ-induced diabetes mellitus in rats. Hence, the use of B. aegyptiaca fruit tea is applicable to approach health-promoting.
Uploads
Papers by Hoda B Mabrok