Papers by joud shafiq
Mycobacterial strains fromtheMycobacterium aviumcomplex were compared witheachotherandwith Mycoba... more Mycobacterial strains fromtheMycobacterium aviumcomplex were compared witheachotherandwith Mycobacterium phlei isolates byrestriction endonuclease digestion ofchromosomal DNAwithSspIandanalysis bypulsed-field gelelectrophoresis. Characteristic profiles were observed forknowntyped strains, andfive groups were identified. Primary bovine isolates identified as Mycobacterium paratuberculosis byclassical methods were showntofall into boththeM.paratuberculosisandM.avium-like groups.M.paratuberculosis 18was inthelatter category. TwoMycobacterium intracellulare strains ofdifferent Schaefer serotypes had different digestion profiles. Inaddition, this system was exploited forthepreparation ofDNA probesbythe isolation, digestion, andsubcloning ofDNA fragments separated bypulsed-field gelelectrophoresis. Probe JC12hybridized onlytoM.aviumcomplex strains, butnottoM.phlei, showing characteristic hybridization profiles foreachofthegroupspreviously identified bypulsed-field gelelectrophoresis. Thea...
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Infection and Immunity, 1994
Plasmodium falciparum malaria in humans is associated with an increase in the percentage and abso... more Plasmodium falciparum malaria in humans is associated with an increase in the percentage and absolute number of gamma delta T cells in the peripheral blood. This increase begins during the acute infection phase and persists for at least 4 weeks during convalescence. In the present study, 25 to 30% of the gamma delta T cells expressed HLA-DR antigens in vivo and in some patients they proliferated in response to further stimulation by purified human interleukin 2 in vitro. However, there was no in vitro proliferative response to various malarial antigens, including a 75-kDa heat shock protein and a 72-kDa glucose-regulated protein of P. falciparum during the acute infection phase. Cytofluorographic studies showed that although an increase of V delta 1- gamma delta T cells was largely responsible for the expansion of the total number of gamma delta T cells, there was also a proportional increase in V delta 1+ cells. These results were confirmed with anchored PCR and by DNA sequencing t...
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Abstract Submitted to the Faculty of Graduate Studies and Research in partial fulfilment of the r... more Abstract Submitted to the Faculty of Graduate Studies and Research in partial fulfilment of the requirements for the degree of Doctor of Philosophy, Department of Medical Microbiology and Immunology.. Thesis (Ph. D.)--University of Alberta, 1997.
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Papers by joud shafiq
Teaching Documents by joud shafiq