Carboxymethylenebutenolidase

carboxymethylenebutenolidase
Identifiers
EC number	3.1.1.45
CAS number	Template:CAS
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles NCBI proteins

In enzymology, a carboxymethylenebutenolidase (EC 3.1.1.45) is an enzyme that catalyzes the chemical reaction

4-carboxymethylenebut-2-en-4-olide + H₂O $\rightleftharpoons$ 4-oxohex-2-enedioate

Thus, the two substrates of this enzyme are 4-carboxymethylenebut-2-en-4-olide and H₂O, whereas its product is 4-oxohex-2-enedioate.

This enzyme belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is 4-carboxymethylenebut-2-en-4-olide lactonohydrolase. Other names in common use include maleylacetate enol-lactonase, dienelactone hydrolase, and carboxymethylene butenolide hydrolase. This enzyme participates in gamma-hexachlorocyclohexane degradation and 1,4-dichlorobenzene degradation.

Structural studies

As of late 2007, 10 structures have been solved for this class of enzymes, with PDB accession codes 1DIN, 1GGV, 1ZI6, 1ZI8, 1ZI9, 1ZIC, 1ZIX, 1ZIY, 1ZJ4, and 1ZJ5.