Nucleic acid methods

For protein methods, see Protein methods.

Nucleic acid methods are the techniques used to study nucleic acids (DNA and RNA).

Purification

• Phenol–chloroform extraction
• Minicolumn purification
• RNA extraction
• Boom method

Quantification

• Abundance in weight: spectroscopic quantification
• Absolute abundance in number: quantitative PCR
• High-throughput relative abundance: DNA microarray
• High-throughput absolute abundance: SAGE
• Size: gel electrophoresis

Synthesis

• De novo: oligonucleotide synthesis
• Amplification: PCR

Kinetics

• Dual polarization interferometry
• QCM-D

Other

• Bisulfite sequencing
• DNA sequencing
• Expression cloning
• Fluorescence in situ hybridization
• Lab-on-a-chip
• Nucleic acid simulations
• Northern blot
• Nuclear run-on assay
• Radioactivity in biological research
• Southern blot
• Sucrose gradient centrifugation
• Several bioinformatics methods, such as RNA structure prediction

See also

• CSH Protocols
• Current Protocols

External links

• Protocols for Recombinant DNA Isolation, Cloning, and Sequencing