Khalid Sait

King AbdulAziz University (KAU) Jeddah, Saudi Arabia, Department of Obstetrics and Gynecology, Faculty Member

Followers

Following

Co-authors

Mentions

161

Public Views

Professor and gynecological oncologiest Laparoscopy surgery. Canadian certification King abdulaziz universityJeddah saudi arabia
Address: Jeddah, Makkah Province, Saudi Arabia

less

Peter MacCallum Cancer Centre

Ming-Yin Lin

Sheng-mou Hsiao

Setareh Akhavan

Tehran University of Medical Sciences

Maria Del Pilar Estevez Diz

Universidade de São Paulo

Susie Lau

Apichai Vasuratna

InterestsView All (15)

Uploads

Papers by Khalid Sait

Chemosensitizing effect of aqueous extract of sweet fennel in cisplatin treated HeLa cells

Gynecologic Oncology, 2015

Objectives: Cisplatin is an important chemotherapeutic agent that is widely used in treatment aga... more Objectives: Cisplatin is an important chemotherapeutic agent that is widely used in treatment against several malignancies, but the adverse effects in normal tissues and organs limit its use. The aim of this study was to evaluate the effect of aqueous extract of sweet fennel alone and in combination with cisplatin on a human cervical cancer adenocarcinoma cell line (HeLa cells) in a search for an effective, cost-effective therapy with minimal adverse effects. Methods: The HeLa cell line was used to study the cytotoxic effect of different concentrations of the aqueous extract of sweet fennel alone and in combination with 50 mcg/mL cisplatin. Drug interaction was quantified by the combination index. Gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC) were used to analyze the components of the sweet fennel decoction. MTT assay was used to examine cell viability percentage. Electron microscopy was applied to study the ultrastructure of the cells. Results: The phenyl propanoids (23%) and phenols (12%) constituted the highest percentage of the aqueous extract. Increasing the concentration of sweet fennel from 50 mcg/mL to 80 mcg/mL decreased the percentage of cell viability of HeLa cells from 86.74% to 78.28%, respectively. Further decrease to 11.31% was demonstrated when 50 mcg/mL of fennel was combined with 50 mcg/mL cisplatin (additive effect). In addition to the signs of apoptosis observed in HeLa cells at 50 mcg/mL of fennel, disruption of both nuclear and cytoplasmic membranes and presence of autophagolysosomes were noticed at a dose of 80 mcg/mL. Combining 50 mcg/mL of cisplatin with 60, 70, and 80 mcg/mL of sweet fennel revealed no significant difference in comparison to cisplatin alone. The combination with 50 mcg/mL of sweet fennel revealed marked vacuolization of the cytoplasm, fragmentation of the nucleus, and complete disruption of the nuclear membrane. Conclusions: The combination of cisplatin and 50 mcg/mL fennel could enhance inhibition of cervical cancer growth. This combination could be effective in lowering the dose of single or repeated cumulative courses of cisplatin and, hence, decrease its hazardous adverse effects. In vivo studies and the evaluation of different combination doses of cisplatin and sweet fennel are recommended.

Khalid Sait

Uploads

Papers by Khalid Sait

Log In