Key message There are multiple publications on Anthurium transformation, yet a commercial product... more Key message There are multiple publications on Anthurium transformation, yet a commercial product has not been achieved. This may be due to use of nonoptimum promoters here we address this problem. Abstract Different promoters and tissue types were evaluated for transient b-glucuronidase (GUS) expression in Anthurium andraeanum Hort. 'Marian Seefurth' following microprojectile bombardment. Plasmids containing the Ubiquitin 2, Actin 1, Cytochrome C1 from rice, Ubiquitin 1 from maize and 35S promoter from Cauliflower Mosaic Virus fused to a GUS reporter gene were bombarded into in vitro grown anthurium lamina, somatic embryos and roots. The number of GUS foci and the intensity of GUS expression were evaluated for each construct. Ubiquitin promoters from rice and maize resulted in the highest number of expressing cells in all tissues examined. Due to the slow growth of anthurium plants, development of transgenic anthurium plants takes years. This research has rapidly identified multiple promoters that express in various anthurium tissues facilitating the development of transformation vectors for the expression of desirable traits in anthurium plants.
ABSTRACT Basil oil, Ocimum basilicum L., is a volatile plant essential oil that is known to have ... more ABSTRACT Basil oil, Ocimum basilicum L., is a volatile plant essential oil that is known to have insecticidal activity against stored product pests such as rice weevil, Sitophilus oryzae (L.). Basil oil was diluted in acetone and applied to a sponge held inside a tea strainer for fumigations in containers with and without rice. Basil oil fumigation (3 ml, 10% concentration) caused high mortality in adult rice weevils when weevils were exposed in air in a sealed 1 L plastic container. However, when basil oil was placed in packaged rice, weevil mortality was low and reproduction was not affected. Effectiveness of plant essential oil fumigation should be conducted under realistic conditions to avoid experimental artifacts and misleading results.
Paret, M. L., Cabos, R., Kratky, B. A., and Alvarez, A. M. 2010. Effect of plant essential oils o... more Paret, M. L., Cabos, R., Kratky, B. A., and Alvarez, A. M. 2010. Effect of plant essential oils on Ralstonia solanacearum race 4 and bacterial wilt of edible ginger. Plant Dis. 94:521-527.
Key message There are multiple publications on Anthurium transformation, yet a commercial product... more Key message There are multiple publications on Anthurium transformation, yet a commercial product has not been achieved. This may be due to use of nonoptimum promoters here we address this problem. Abstract Different promoters and tissue types were evaluated for transient b-glucuronidase (GUS) expression in Anthurium andraeanum Hort. 'Marian Seefurth' following microprojectile bombardment. Plasmids containing the Ubiquitin 2, Actin 1, Cytochrome C1 from rice, Ubiquitin 1 from maize and 35S promoter from Cauliflower Mosaic Virus fused to a GUS reporter gene were bombarded into in vitro grown anthurium lamina, somatic embryos and roots. The number of GUS foci and the intensity of GUS expression were evaluated for each construct. Ubiquitin promoters from rice and maize resulted in the highest number of expressing cells in all tissues examined. Due to the slow growth of anthurium plants, development of transgenic anthurium plants takes years. This research has rapidly identified multiple promoters that express in various anthurium tissues facilitating the development of transformation vectors for the expression of desirable traits in anthurium plants.
ABSTRACT Basil oil, Ocimum basilicum L., is a volatile plant essential oil that is known to have ... more ABSTRACT Basil oil, Ocimum basilicum L., is a volatile plant essential oil that is known to have insecticidal activity against stored product pests such as rice weevil, Sitophilus oryzae (L.). Basil oil was diluted in acetone and applied to a sponge held inside a tea strainer for fumigations in containers with and without rice. Basil oil fumigation (3 ml, 10% concentration) caused high mortality in adult rice weevils when weevils were exposed in air in a sealed 1 L plastic container. However, when basil oil was placed in packaged rice, weevil mortality was low and reproduction was not affected. Effectiveness of plant essential oil fumigation should be conducted under realistic conditions to avoid experimental artifacts and misleading results.
Paret, M. L., Cabos, R., Kratky, B. A., and Alvarez, A. M. 2010. Effect of plant essential oils o... more Paret, M. L., Cabos, R., Kratky, B. A., and Alvarez, A. M. 2010. Effect of plant essential oils on Ralstonia solanacearum race 4 and bacterial wilt of edible ginger. Plant Dis. 94:521-527.
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