Papers by DR. NAGENDRA K U M A R CHANDRAWANSHI
Research Journal of Biotechnology, 2024
Laetiporus sulphureus (Bull.) Murrill belongs to the family of Polyporaceae which appears striden... more Laetiporus sulphureus (Bull.) Murrill belongs to the family of Polyporaceae which appears strident orange or sulfur yellow colouration, commonly known as chicken of the wood, which is found in hardwood and is cautiously consumed by humans. Recent research revealed that it is a prominent source of bioactive compounds having potential sources for antioxidants and other pharma-active components. Thus, the present objectives are to determine and to choose the potential polar solvent for extract preparation and then to validate the potency of the antioxidant and antidiabetic properties. The current studies employed various polar solvents for extract preparation. The experimentation recorded that the methanol solvent had shown a maximum yield of extracts (11.05±0.05 (g /100 dry weights). The obtained extracts were tested and significant results were obtained for antioxidant and antidiabetic assays. It has been recorded as a decrease dose with increasing order of concentration of extracts with a total of 50% inhibition (IC50). The studies suggested that L. sulphureus has therapeutic and nutritional values, which will be used for various human ailments and provide alternative sources for the pharmaceutical industry instead of chemically synthesized materials.
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Research Journal of Chemistry and Environment, 2024
Heavy metal contamination in drinking water causes serious health issues. The hazards of heavy me... more Heavy metal contamination in drinking water causes serious health issues. The hazards of heavy metals, especially iron contamination affect human health such as skin roughness, the stickiness of hair, weakness of teeth etc. The objective is to study the heavy metals contaminated water remediation process by mushroom cultivated bags. The mushroom cultivation used agro wastes; the cultivated residues were known as spent mushroom substrates. In the present experiment, Calocybe indica spent mushroom substrates to treat heavy metal contamination from drinking water. The iron-contaminated water concentration rapidly decreased from 0.231±0.01 to 0.134±0.00 with maximum exposure periods. The time exposures, or contact time, have increased and removal efficiency has improved along with the incubation time. The study data inferred more heavy metal adsorption than untreated water samples. An initial pH range of 4.0 to 5.0, a contact duration of 10 minutes, an initial iron (III) concentration of 50 mg/L and an absorbed dose half-saturation constant of 0.4 g were used. The current study showed that the increasing pH of the medium enhanced the adsorption capacity of the spent mushroom substrate. Similarly, the time exposures, or contact time, have increased and removal efficiency has grown along with the incubation time. These findings underscore the potential of utilizing spent mushroom substrates as an economical and portable biosorbent for rural water treatment applications.
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International Journal of Emerging Technologies and Innovative Research, 6(5): 473-477, 2019
G. lucidum is commonly known as 'Reishi.' It has a variety of bioactive compounds. Aspergillus ni... more G. lucidum is commonly known as 'Reishi.' It has a variety of bioactive compounds. Aspergillus niger is common fungi, categorized with GRAS (generally recognized as safe) status by food and drug administration. However, due to its cosmopolitan nature, human beings get frequently exposed to spores and vegetative forms of A.niger. Mushroom has a wide diversity of bioactive components; the compound extraction process has an essential selection of suitable solvents for particular bioactive products for higher targetted efficiency activity. The present study took various organic and aqueous mediums for extract preparation. The rapid in vitro antifungal activity was analyzed. Among them, ethanolic extracts have shown potential, followed by other solvents in normal water, methanol, chloroform, and hot water. The current study result interpreted that owing to the new attraction to the properties of new antifungal products like fighting multidrug-resistant fungus. It is essential to develop a better understanding of the current methods available for screening and quantifying the antifungal effect of an extract or a pure compound for its applications in human health. IndexTerms-Reishi, bioactive compounds, good solvent, in vitro, antifungal activity.
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NewBioWorld, 1(1):5-8, 2019
Schizophyllum commune is well-known, non-consumable split gill mushroom having a variety of nutra... more Schizophyllum commune is well-known, non-consumable split gill mushroom having a variety of nutraceutical properties, due to the presence of various bioactive components. In this study, antidiabetic potency of Schizophyllum commune was evaluated using different extraction solvents. Results revealed the extract of methanol solvent showed high antidiabetic potency (IC50 =50.98 μg/ml), followed by another solvents such as ethanol and hot water extracts respectively. Antidiabetic activity of extracts was compared with standards and positive correlation was observed. In conclusion, based on accumulated data Schizophyllum commune may be used as natural antidiabetic substance and a better alternative to synthetic chemicals. It can be prospective source of potent constituent for fighting against human metabolic disorder.
Keywords: Split gill mushroom, Antidiabetic assay, Extraction solvent, Metabolic disorder.
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Research J. Pharm. and Tech. 11(12), 2018
Daedaleopsis confragosa belongs to the family of Polyporaceae, which is a highly variable species... more Daedaleopsis confragosa belongs to the family of Polyporaceae, which is a highly variable species, presence maze-like pores, commonly whitish to brownish cap with a zone of color and its pore surface to bruise reddish, which is found in hardwood and stumps. The flesh is very tough nature, not consumed by the human. Although the recent research revealed that it is having the prominent sources of antioxidants and other pharma bio-active component. So, therefore, the present study performed to in vitro evaluation of antioxidant and antidiabetic efficieny. In the present studies, using a various polar solvent for extracts preparation. The experimentations were recorded that the hot water solvent had shown a maximum yield of extracts 50.00 (g/weight of defatted dried mushroom). The significant results were recorded for antioxidant and antidiabetic capacity in different measuring methods. The methanolic extracts (CME) showed the strongest DPPH radical scavenging activity (94.39±0.01), the water extract gave a highest reducing power (84.12±0.00) and maximum hydrogen peroxide inhibitory found for hot water extracts (CHE) (99.32±0.03 at 100 μg/mL. The observed values were expressed in decrease dose-dependently with increasing order of concentration of the extracts with IC50. The studies are suggested that the D. confragosa is having enormous therapeutic and nutritive values, which will be used for various human ailments and also provide alternative sources for the pharmaceutical valued.
KEYWORDS: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
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Schizophyllum commune belongs to the large and remarkable group of mushroom and it is known as sp... more Schizophyllum commune belongs to the large and remarkable group of mushroom and it is known as split gill mushroom. It is an unexplored and non-consumed by the human. Recent research revealed that S. commune emerges as a richness for various antioxidants and other pharma active component. In the present studied, hot water, methanol, and ethanol crude extracts of S. commune were investigated for their antioxidant capacity by using various assay methods and also determined antidiabetic property. Ethanolic extract showed ample potential for antioxidant activity, in the DPPH scavenging method recorded higher inhibition (IC 50 =18.56 µg/ml), other than in hot water and methanol extracts. For the reducing power assay, hot water extract showed great scavenging activity recorded (IC 50 =20.00 µg/ml) comparison to another extract. Ethanolic extract showed most potent H 2 O 2 scavenging activity in the assay; which was showed (IC 50 =19.79 µg/ml) and similarly total phenolic content was given significantly values for ethanolic extract (IC 50 =11.86 µg/ml), comparatively methanol and hot water extracts. Positive correlation was found between to all extracts medium and their antioxidant activities. The studied revealed that split gill mushroom may have potential sources of natural antioxidant and antidiabetic stuff, therefore may be utilized as promising sources of therapeutics.
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Gloriosa superba L. is an herbaceous climber distributed in tropical parts of the
world. Pharmace... more Gloriosa superba L. is an herbaceous climber distributed in tropical parts of the
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
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This article gives an overview of categorization of threatened status of Gloriosasuperba L., medi... more This article gives an overview of categorization of threatened status of Gloriosasuperba L., medicinal plant in the present status. The available literature related to these aspects drawn from studies done in various interest areas of threatened condition viz. extinct, critically endangered and endangered, vulnerable (VU), data deficient (DD), low risk (LR) and not evaluated (NE). The supporting literature obtained from standard web sources and available authentic data from national and international research agencies. The relative analysis is definitely promoted to actual classifying of G. superbaL. in national, IUCN system and as well an international database. Throughout to individual afford required of a botanist, biotechnologist, conservationists and policy makers , an obligatory to conserve it and develop techniques for in-situ and ex-situ conservation. Further, the threatened medicinal plant will be available for future, accordingly essential need a development method for molecular study, germplasm conservation and phytochemistry etc.
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The medicinal plants are widely used by the traditional medical practitioners for curing various ... more The medicinal plants are widely used by the traditional medical practitioners for curing various diseases in their day to day practice. In traditional systems of medicine, different parts (leaves, stem, flower, root, seeds and even whole plant) of Ocimum sanctum L. (Known as Tulsi in Hindi), a small herb seen throughout India and in Chhattisgarh region two types found black and green leaves Ocimum. The objective of study to find out better species of green or black leaves of Ocimum. Phytochemical screenings carried out of green and black leaves founds to dissimilar patterns of chemical constituents and region also differs in antimicrobial activity against to Staphylococcus aureus and E. coli. respectively. Consequently green leaves of Ocimum showed to better response of phytochemical constituent as well as antimicrobial activity as compare to black Ocimum leaves.
Keywords: Green leaves, black leaves, phytochemical constituents, antimicrobial activity.
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Present paper deals the study of MIC using a serial dilution method. The antibacterial activity d... more Present paper deals the study of MIC using a serial dilution method. The antibacterial activity determined against gram-positive bacteria and was isolated from soil samples of biotechnology department of Pandit Ravishankar Shukla University, Raipur. For this study 10 different Penicillium species were employed viz. P. citrinum, P. purpurogenum, P. notatum, P. rubrum, P. oxalicum, P. frequentans, P. funiculosum, P. variabile, P. rugulosum and P. chrysogenum. In the present investigation it was observed that whenever increasing the concentration of antibacterial substance to medium produce a less population of test bacteria. Bacterial growth was determined by spectrophotometer at 600 nm. Among all, three Penicillium species viz. [P. purpurogenum (0.043), P. rubrum (0.039) and P. oxalicum (0.068)] showed good results. Whereas other Penicillium species showed moderate antibacterial property.
Keywords: Penicillium, MIC, Antibacterial activity.
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Conference Presentations by DR. NAGENDRA K U M A R CHANDRAWANSHI
Daedaleopsis confragosa belongs to the family of Polyporaceae, which is a highly variable species... more Daedaleopsis confragosa belongs to the family of Polyporaceae, which is a highly variable species, presence maze like pores, commonly whitish to brownish cap with zone of color and its pore surface to bruise reddish, which is found in hardwood and stumps. The flesh is very tough nature, not consumed by human. But in the recent research revealed that it is having the prominent sources of antioxidants and other pharma bio active component. So, therefore, the present study performed to in vitro evaluation of antioxidant and correspondingly determined the phytochemical of the mushroom. In the present studies, used various polar solvent for extracts preparation. The experimentation were recorded that the hot water solvent had shown a maximum yield of extracts. The significant results were recorded for antioxidant and phytochemical assayed. The recorded values decrease dose dependently with increasing order of concentration of extracts with IC50. The studies are suggested that the D. confragosa having therapeutic and nutritive values. The outcome of the study, will be supported for development and isolation of new bioactive component from D. confragosa, which will be used for various human ailments and also provide alternative sources for pharmaceutical industry instead of chemically synthesized materials.
Keywords: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
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Glory lily (Gloriosa superba L.) is an herbaceous climber and globally interested important medic... more Glory lily (Gloriosa superba L.) is an herbaceous climber and globally interested important medicinal plant, due to the presence of pharma active ingredient colchicine. G. superba is distributed in tropical parts of India. It possesses a strong apical dominance character in nature, which is a notable reason for pushing it in threaten category. The present study focused on development of microtuberization technique for conservation and propagation of Glory lily. The meristematic regions of underground tubers were selected as explants. The in vitro study was carried on MS medium containing different combination of plant growth regulator (PGR) and other adjuvant such as citric acid, Polyvinyl pyrrolidone-40 and sucrose. Maximum number of microtubers were obtained in the presence of BA and containing higher level of sucrose in the culture medium. The regenerated microtubers were produced shoots; they were get separated and subjected to in vitro hardening. The in vitro hardened plantlets with functional roots and photo synthetically active leaves were successfully transferred to green house with 95 % survival.
Keywords: Glory lily, In vitro conservation, Medicinal plant, Microtuberization, Plant growth regulator.
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Thesis Chapters by DR. NAGENDRA K U M A R CHANDRAWANSHI
International Publications, Kanpur, India (ISBN: 978-81-945988-3-1), 2020
The Indian economy is the fastest growing economy in the globe. The economic revolution is signif... more The Indian economy is the fastest growing economy in the globe. The economic revolution is significantly depending on the performance of agriculture and other allied sectors. The agriculture practices are the most important custom tradition developed by human beings for survival on the earth at ancient. The most population proportion of India belongs to rural areas. Thus, majority of the population depend on indirectly or directly in agriculture practices for employment opportunities. According to the food and agriculture organization (FAO) report for India, as 70% of rural population depend on primary employment as agriculture, among to them only 82% of farmers have small and marginal level. The population will continually increasing in last decade. Therefore some emerging challenges has appears such as huger rate, malnutrition, contaminated food products, along with reduced agriculture land, loss of agriculture productivity, less fertile, climate changes, pest be resistance, due to heavy used in chemical or pesticides in era of green revolution. The objective of this chapter, to deal with inclusive economic development through sustainable practices in agriculture, development of bio economy, implementing of advance and biological practices in cropping fields, furthermore, to prepares the future policy to incorporating in developed country schemes for betterment for economic and social empowering
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Papers by DR. NAGENDRA K U M A R CHANDRAWANSHI
Keywords: Split gill mushroom, Antidiabetic assay, Extraction solvent, Metabolic disorder.
KEYWORDS: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
Keywords: Green leaves, black leaves, phytochemical constituents, antimicrobial activity.
Keywords: Penicillium, MIC, Antibacterial activity.
Conference Presentations by DR. NAGENDRA K U M A R CHANDRAWANSHI
Keywords: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
Keywords: Glory lily, In vitro conservation, Medicinal plant, Microtuberization, Plant growth regulator.
Thesis Chapters by DR. NAGENDRA K U M A R CHANDRAWANSHI
Keywords: Split gill mushroom, Antidiabetic assay, Extraction solvent, Metabolic disorder.
KEYWORDS: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
world. Pharmaceutically important alkaloid-colchicine, present in its tubers and
seeds and due to overexploitation it becomes vulnerable in the forests. In the
present investigation, in vitro tuber production was carried out for its propagation
and conservation. The plant possesses a very strong apical dominance.
Consequently, any damage to the plant apical meristem is fatal for it which was also
exhibited during in vitro culture. Only apical meristems were able to produce a
single and un-branched shoots and nodal explants were remained dormant even in
the presence of exogenous cytokinin. The in vitro propagation was accomplished
by the microtuber formation technique, in two steps. Maximum number of
microtubers 9.8±0.8 per culture in eight weeks, were produced in vitro on
Murashige and Skoog medium with sucrose (60 g LG1) and in the presence
of 35.5 μM 6-benzyladenine (BA) with citric acid and polyvinyl pyrrolidone-40.
Subsequently the induced microtubers were sub-cultured on to the medium with
lower cytokinin level, 8.88 μM BA. The individual microtubers with shoots were
subjected to a single step rooting and in vitro acclimatization in coco-pit containing
vessels, exhibited 90% survival. In vitro grown tubers contained less percentage of
colchicine than the natural field-grown plant tubers. However, microtubers showed
increased colchicine content, as they grow older.
Key words: Apical dominance release, bud culture, cytokinin, glory lily
Keywords: Green leaves, black leaves, phytochemical constituents, antimicrobial activity.
Keywords: Penicillium, MIC, Antibacterial activity.
Keywords: Polyporaceae, Antioxidant, Antidiabetic, Polar solvent and Human ailments.
Keywords: Glory lily, In vitro conservation, Medicinal plant, Microtuberization, Plant growth regulator.