Suresh Kumar
I am Dr. S. Sureshkumar, working as assistant professor (Biotechnology) in Faculty of Agricultural Research, Sri Ramakrishna Mission Vivekananda Education and Research Institute, Periyanaicken palayam, Coimbatore, Tamilnadu. Specialization with marker assisted breeding.
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Maize is an important food and feed crop worldwide. Phytic Acid (PA), in maize kernel, is an anti-nutritional factor. PA chelates mineral cations and causesmineral deficiency in humans and phosphorous deficiency in animals. The undigested PA excreted by monogastric animals, causes phosphorous eutrophication. Therefore, development of low phytate maize is indispensable. The low phytate locus (lpa2 allele) has been transferred from low phytate mutant line “EC 659418” into an elite inbred UMI 395 through Marker Assisted Backcross Breeding (MABB). The MABB involved three backcrosses followed by two selfing steps, includes “foreground selection” i.e., selecting lines with lpa2 allelewith the help of a co-dominant SSR marker “umc2230” and “background selection” i.e., selecting plants having genetic background similar to that of the recurrent parent using 50 co-dominant SSR markers. Two low phytate lpa2lineswith genome similar (> 90% similarity) to that of recurrentparent have been identified.These lines can be used as parent in future hybridization programs for obtaining low phytate high yielding maize hybrids.
Maize is an important food and feed crop worldwide. Phytic Acid (PA), in maize kernel, is an anti-nutritional factor. PA chelates mineral cations and causesmineral deficiency in humans and phosphorous deficiency in animals. The undigested PA excreted by monogastric animals, causes phosphorous eutrophication. Therefore, development of low phytate maize is indispensable. The low phytate locus (lpa2 allele) has been transferred from low phytate mutant line “EC 659418” into an elite inbred UMI 395 through Marker Assisted Backcross Breeding (MABB). The MABB involved three backcrosses followed by two selfing steps, includes “foreground selection” i.e., selecting lines with lpa2 allelewith the help of a co-dominant SSR marker “umc2230” and “background selection” i.e., selecting plants having genetic background similar to that of the recurrent parent using 50 co-dominant SSR markers. Two low phytate lpa2lineswith genome similar (> 90% similarity) to that of recurrentparent have been identified.These lines can be used as parent in future hybridization programs for obtaining low phytate high yielding maize hybrids.