Papers by Armando Mena-Duran
Journal of atrial fibrillation, 2013
Background: The present multicentre study was aimed at determining the effect of preoperative atr... more Background: The present multicentre study was aimed at determining the effect of preoperative atrial fibrillation (preop-AF) as stroke risk factor in coronary artery bypass graft surgery (CABG) during the perioperative period. Methods: Patients undergoing isolated CABG surgery were enrolled from 21 Spanish centers. Baseline variables related with perioperative stroke risk were recorded and analysed. The Northern New England Cardiovascular Disease Study Group (NNECVDSG) stroke risk schema was used to stratify stroke risk and compare predicted vs observed neurologic outcomes in this study. Results: 26347 patients were enrolled in the study. Prevalence of preop-AF was 4.2%, and was associated significantly with major cardiovascular comorbidities. The stroke rate was 1.38% (365 strokes), and it was slightly higher for patients with preop-AF vs non preop-AF, 1.82% vs 1.36%, p = 0.2. NNECVDSG schema showed good predictive ability calculating the area under the receiver operating character...
Therapeia Estudios Y Propuestas En Ciencias De La Salud, 2011
El nuevo virus xenotrópico relacionado con el virus de la leucemia murina (XMRV), único gamma ret... more El nuevo virus xenotrópico relacionado con el virus de la leucemia murina (XMRV), único gamma retrovirus identificado en humanos hasta la fecha, se aisló de especímenes de cáncer de próstata en el año 2006, y en el 2009 se asoció impactantemente con el síndrome de fatiga crónica (SFC), enfermedad debilitante de sintomatología compleja que cursa, frecuentemente, con alteraciones de la enzima antiviral ribonucleasa L (RNasa L), al igual que el cáncer de próstata hereditario. La imposibilidad de replicar estos hallazgos y la presencia de secuencias virales similares halladas en kits comerciales y preparaciones de polimerasas "hotstart" que contienen anticuerpos derivados de hibridomas plantean la posibilidad de que las secuencias amplificadas en los estudios iniciales del XMRV puedan corresponder a contaminaciones con ADN murino y que el nuevo XMRV no sea más que un artefacto creado por procesos de recombinación en el seno de un xenoinjerto. Sin embargo, una contaminación casual no permitiría explicar la desigual distribución de secuencias virales halladas entre pacientes y controles sanos, ni cómo fue posible hallar secuencias genómicas específicas humanas flanqueando las secuencias terminales repetidas largas (LTRs) virales del XMRV en tejidos de pacientes de cáncer de próstata.
Annals of the Rheumatic Diseases, 2013
Blood, 2006
Recently, Chen et al (J Biol Chem.2005;280:5361–9) showed SHP-1 (PTPN6) gene supression by FLT3/I... more Recently, Chen et al (J Biol Chem.2005;280:5361–9) showed SHP-1 (PTPN6) gene supression by FLT3/ITD in TF-1 cell lines and in primary cells from AML patients. To address the prognostic impact that both together FLT3/ITD and SHP-1 gene suppression may have in myeloid malignancies patients, we have studied 85 patients diagnosed with de novo AML and MDS. SHP-1 gene expression was quantified by real time PCR and compared with normal CD34+ cells from healthy donors. FLT3/ITD was detected by conventional agarose gel electrophoresis after PCR. FLT3/D835 was detected by melting curve methodology. FLT3 mutations were correlated with higher WBC and blasts count at diagnosis (p=0.001). FLT3/ITD had a negative impact on DFS compared with wt or D835 mutations (log-rank p=0.054). Median levels for SHP-1 were lower in leukemic blasts than in normal peripheral blood CD34+ cells (p=0.001). SHP-1 lowest expression was found in those patients where FLT3 mutations were present (p=0.001). SHP-1 lowest e...
Acute promyelocytic leukemia (APL) is genetically characterized by a reciprocal translocation be-... more Acute promyelocytic leukemia (APL) is genetically characterized by a reciprocal translocation be- tween chromosomes 15 and 17, the t(15;17)(q22;q21), which results in the fusion gene PML/RARA. A small proportion of patients with APL have complex or simple variants of this translocation. We report the case of a 31-year-old woman with APL (FAB-M3 classical form) carrying an apparently balanced translocation t(17;20)(q21;q12) masking a t(15;17)(q22;q21) confirmed by fluorescence in situ hybridization (FISH) and molecular studies. The patient was treated with an all-trans-retinoic acid (ATRA) plus anthracycline-based protocol and achieved complete remission, with no recur- rence to date. These results illustrate the usefulness of combining cytogenetics, FISH, and reverse transcription-polymerase chain reaction (RT-PCR) methods to evidence the PML/RARA fusion gene in cases with morphologic suspicion of APL with variant or cryptic t(15;17). 2006 Elsevier Inc. All rights reserved.
Blood
Tumour lysis syndrome (TLS) can be a life threatening complication during induction chemotherapy ... more Tumour lysis syndrome (TLS) can be a life threatening complication during induction chemotherapy in patients with acute myeloid leukaemia (AML). However, its incidence, risk stratification and management strategies are based on data mainly from lymphoid malignancies. Risk-adapted prophylactic strategies for TLS are in need with the introduction agents like Rasburicase. This single-centre retrospective chart review study was conducted to identify TLS predictive factors in a large AML population undergoing first induction chemotherapy. Between January 1980 and December 2000, 614 consecutive adult patients were diagnosed of de novo AML and started therapy in our institution. Prophylaxis of TLS consisted of intravenous hydration and oral allopurinol. Laboratory TLS (LTLS) was defined as either a 25% change from baseline or level above upper laboratory normal values (ULN)…
Blood
Background. AML conventional chemotherapy followed or not by autologous stem cell transplantation... more Background. AML conventional chemotherapy followed or not by autologous stem cell transplantation could be curative for high risk MDS and sAML. Aim. To evaluate outcome in 103 patients enrolled in PETHEMA’s FLAG-IDA protocol achieving complete remission (CR) followed by intensive chemotherapy and autologous transplantation compared to those with no further treatment. Patients and methods. 103 patients were recruited from 15 institutions starting December 1997 till December 2004. Eligibility criteria: de novo MDS with Spanish score >2 and/or International Prognostic Scoring System (IPSS) >1; or sAML. Induction chemotherapy was the FLAG-IDA regime (Fludarabine, cytarabine (ARA-C), idarubicin (IDA) and G-CSF). Patients achieving complete remission (CR) had consolidation chemotherapy with IDA+ARAC+G-CSF. Patients younger than 65 yrs old who mobilized enough hematopoietic progenitors proceeded to autologous stem cell transplantation. Poor mobilizers were treated further either with...
Blood
The protein-tyrosine-phosphatase (PTPase) SHP-1 is expressed almost exclusively in hematopoetic c... more The protein-tyrosine-phosphatase (PTPase) SHP-1 is expressed almost exclusively in hematopoetic cells. Most growth factor receptors rely on the Jak/Stat pathway for intracellular transduction, to drive specific gene expression which are involved in cell proliferation and differentiation. Abnormal signaling in some of these pathways has been linked to hematopoietic malignancies, like the ALL tel-Jak2 gene fusion. In some other cases activation is mediated by kinases not normally associated with Stats, like abl. Recently several authors have linked dysregulation of PTPases, especially SHP-1, to non-Hodgkin lymphoma, familial polycythemia, chronic neutropenia and AML. There are no reports on expression of SHP-1 in myelodysplastic syndromes. Here we hypothesize that SHP-1 downregulation may play a role in the leukemic transformation of myelodysplastic syndromes and may impact survival. Retrospectively, from January 1990 to January 2004, we studied 45 patients (29 men, 16 women; median a...
Page 1. DEPARTAMENT DE MEDICINA INTERACCIÓN DE LA PROTEÍNA-TIROSINA FOSFATASA SHP-1 Y LA PROTEÍNA... more Page 1. DEPARTAMENT DE MEDICINA INTERACCIÓN DE LA PROTEÍNA-TIROSINA FOSFATASA SHP-1 Y LA PROTEÍNA-TIROSINA KINASA FLT3 CONSTITUTIVAMENTE ACTIVADA EN LAS ENFERMEDADES MALIGNAS MIELOIDES: UN NUEVO FACTOR PRONÓSTICO ...
Http Dx Doi Org 10 1080 08905767008593691, May 11, 2012
Review: Literature and Arts of the Americas, 1970
Interactive CardioVascular and Thoracic Surgery, 2016
We aim to describe our experience in coronary artery bypass grafting (CABG) with or without cardi... more We aim to describe our experience in coronary artery bypass grafting (CABG) with or without cardiopulmonary bypass by comparing intraoperative and postoperative outcomes. From January 1993 to June 2013, 3097 patients underwent consecutive emergency and scheduled CABG surgery. A total of 1770 patients underwent on-pump CABG (ONCABG) and 1327 off-pump CABG (OPCABG). A propensity score matching was performed to identify appropriate matched-pair patients; univariable and multivariable logistic regression analyses were performed to assess significant predictors of hospital and 30-day morbidity and mortality composite end-points. Morbidity composite end-point was defined as any renal, pulmonary, cardiovascular and neurological complication that occurred during hospital stay. We collected all-cause mortality data during the study period. We identified 1004 patients in each group. There were no significant differences in thirty day mortality, 2.8 vs 3.8%, in OPCABG and ONCABG, respectively (P = 0.21). Cardiovascular, neurological, respiratory and renal complications were more frequent in the ONCABG group: 13.9 vs 8.7% (P < 0.001), 3.9 vs 2.2% (P = 0.03), 13.5 vs 7.5% (P < 0.001), 7.1 vs 5.3% (P = 0.095), respectively. The long-term all-cause mortality rate was 12.3 vs 12.9% in the OPCABG versus ONCABG group (P = 0.42), respectively. In both uni- and multivariable analysis preoperative renal failure, chronic obstructive pulmonary disease and ONCABG were independent predictors of mortality and morbidity composite end-points. OPCABG is associated with less postoperative morbimortality and shorter hospital and intensive care unit length of stay. ONCABG resulted as an independent predictor of morbidity and mortality composite end-point. No statistically significant differences were observed in long-term all-cause mortality between groups.
Thrombosis and haemostasis, 2005
Schattauer GmbH - Verlag für Medizin und Naturwissenschaften ist ein renommierter Herausgeber von... more Schattauer GmbH - Verlag für Medizin und Naturwissenschaften ist ein renommierter Herausgeber von Fachbüchern, -zeitschriften und -software. Besuchen Sie unser Internetangebot mit Onlinezugang zu unseren Zeitschriftenartikeln.
PLOS ONE, 2015
Diagnosis of fibromyalgia (FM), a chronic musculoskeletal pain syndrome characterized by generali... more Diagnosis of fibromyalgia (FM), a chronic musculoskeletal pain syndrome characterized by generalized body pain, hyperalgesia and other functional and emotional comorbidities, is a challenging process hindered by symptom heterogeneity and clinical overlap with other disorders. No objective diagnostic method exists at present. The aim of this study was to identify changes in miRNA expression profiles (miRNome) of these patients for the development of a quantitative diagnostic method of FM. In addition, knowledge of FM patient miRNomes should lead to a deeper understanding of the etiology and/or symptom severity of this complex disease.
Virology Journal, 2013
Background: The etiology of fibromyalgia and chronic fatigue syndrome (FM/CFS) is currently unkno... more Background: The etiology of fibromyalgia and chronic fatigue syndrome (FM/CFS) is currently unknown. A recurrent viral infection is an attractive hypothesis repeatedly found in the literature since it would explain the persistent pain and tiredness these patients suffer from. The initial striking link of two distinct orphan retroviruses: the gamma retroviruses murine leukemia virus (MLV)-related virus and the delta retrovirus T-lymphotropic virus type 2 (HTLV-2) to chronic fatigue have not been confirmed to date. Results: Genomic DNA (gDNA) from 75 fibromyalgia patients suffering from chronic fatigue and 79 age-matched local healthy controls were screened for the presence of MLV-related and HTLV-2 related proviral sequences. The XMRV env gene was amplified in 20% of samples tested (24% patients/15% healthy controls). Unexpectedly, no PCR amplifications from independent gDNA preparations of the same individuals were obtained. None of the positive samples showed presence of contaminating murine sequences previously reported by other investigators, neither contained additional regions of the virus making us conclude that the initial env amplification came from spurious air-driven amplicon contaminants. No specific HTLV-2 sequences were obtained at any time from any of the 154 quality-controlled gDNA preparations screened.
Molecular Immunology, 2004
The molecular mechanisms of signal transduction have been the focus of intense research during th... more The molecular mechanisms of signal transduction have been the focus of intense research during the last decade. In T cells, much of the work has centered on protein tyrosine kinase-mediated signaling from the TCR and cytokine receptors, while the study of protein tyrosine phosphatases has lagged behind. Nevertheless, it has now become clear that many protein tyrosine phosphatases play equally important roles in T cell physiology and that no kinase-regulated system would work without the counterbalancing participation of phosphatases. In fact, we have learned that many processes are regulated primarily on the phosphatase side. This minireview summarizes the current state-of-the art in our understanding of the regulation and biology of protein tyrosine phosphatases in T lymphocyte physiology.
Internal Medicine, 2012
Acute liver failure is an unusual complication in multiple myeloma. Here, we report a case of mul... more Acute liver failure is an unusual complication in multiple myeloma. Here, we report a case of multiple myeloma with light chain deposition disease (LCDD) that presented with progressive jaundice due to intrahepatic cholestasis. Diagnosis was made after liver biopsy that showed deposition of kappa light chains occupying perisinusoidal spaces. The patient developed encephalopathy and liver failure and died despite prompt initiation of dexamethasone therapy. The current prognosis of multiple myeloma patients with liver failure due to LCDD is dismal. New therapeutic strategies might improve this condition.
Cancer Genetics and Cytogenetics, 2007
The chromosomal alterations at 11q23 that involve the mixed-lineage leukemia gene (MLL, HTRX1, HR... more The chromosomal alterations at 11q23 that involve the mixed-lineage leukemia gene (MLL, HTRX1, HRX, ALL1) are one of the most common cytogenetic abnormalities in acute leukemia and have been associated with a poor prognosis. Given that not all MLL alterations are seen under conventional cytogenetics or fluorescence in situ hybridization (FISH), it is very important to use molecular techniques to determine the cause of alteration. In this study, we describe two cases of AML in which FISH analysis showed a high-level 11q23 amplification, to confirm if this overexpression may be accompanied by partial tandem duplication of the MLL gene (MLL-PTD). Both patients showed complex karyotype and an unfavorable clinical course. The 11q23 region characterization included conventional cytogenetics, FISH, and comparative genomic hybridization analysis to study the expression patterns of several oncogenes located within the amplified region and detection of partial tandem duplication of the MLL gene by reverse-transcription polymerase chain reaction (RT-PCR) and sequencing. MLL-PTD were detected in the two patients. Moreover, patient 1 showed amplification of the MLL flanking region. Our data suggest that molecular methods such as RT-PCR or sequencing should be used to detect MLL alterations, and that amplification of MLL locus may be extended to its flanking region.
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Papers by Armando Mena-Duran