Papers by Krystyna Skalicka-woźniak
Chemistry & Biodiversity, 2009
The volatile compounds from Peucedanum cervaria (Lap.) L. were obtained by hydrodistillation (HD)... more The volatile compounds from Peucedanum cervaria (Lap.) L. were obtained by hydrodistillation (HD) and headspace solid-phase microextraction techniques (HS-SPME), and then analyzed by GC/MS methods. The composition of samples from a botanical garden was compared with plants collected in the wild. The main compounds of the essential oils of P. cervaria were identified as a-pinene, sabinene, and bpinene (more than 80% of oil). The content of b-myrcene, limonene þ b-phellandrene, and germacrene D was higher than 1%. The in vitro antibacterial activity of the essential oil was evaluated by the agar dilution method against ten reference strains of Gram-positive and Gram-negative bacteria.
Food Chemistry, 2015
This is a PDF file of an article that has undergone enhancements after acceptance, such as the ad... more This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
Natural Product Research, 2013
A simple HPLC method has been used for separation and quantitative analysis of the phenolic acids... more A simple HPLC method has been used for separation and quantitative analysis of the phenolic acids in the methanolic extracts of Ligusticum mutellina aerial parts. Chlorogenic acid was the predominant phenolic acid. Additionally, gallic, p-OH-benzoic, caffeic, p-coumaric and ferulic acids were identified. Moderate antibacterial and antifungal activity (MIC = 1.25-2.5 mg mL(-1)) was observed for the methanol extract of L. mutellina herb received from plants in flowering stage against a broad spectrum of bacteria. Micrococcus luteus, Pseudomonas aeruginosa and Candida spp. were the most sensitive to this plant material. Total phenolic content for the methanol extract of L. mutellina herb received from plants in flowering stage was 1.56 g of chlorogenic acid equivalents/100 g dry weight. The methanol extract of L. mutellina herb received from plants in flowering stage showed antioxidant activity with DPPH (IC50 value of 0.40 mg mL(-1)) and with ABTS (IC50 value of 8.65 mg mL(-1)).
Chemistry & Biodiversity, 2010
Plants of the genus Peucedanum have been used in traditional medicine for a long time to treat di... more Plants of the genus Peucedanum have been used in traditional medicine for a long time to treat different diseases including infectious diseases. The hexane fruits extracts of Peucedanum cervaria and P. alsaticum were examined for antimicrobial activity and analyzed for their fatty acid content. Fatty acid composition of oils were analyzed by GC/FID in methyl ester form. Minimal inhibitory concentrations (MICs) of fatty acid fractions against twelve reference bacterial and yeast strains were performed by the twofold serial microdilution broth method. Fourteen fatty acids were identified. Oleic and linoleic acids were found to be dominant. The extracts from both plants examined exhibited inhibitory effects against Gram‐positive strains tested with different MIC values (0.25–2 mg/ml); however, extract from P. alsaticum possessed stronger antibacterial properties and a broader spectrum. The growth of Gram‐negative bacteria and Candida spp. strains was not inhibited even at the highest e...
Acta Chromatographica, 2008
Peucedanum alsaticum L. were isolated by hydrodistillation (HD) and headspace solid-phase microex... more Peucedanum alsaticum L. were isolated by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME), and then analyzed by GC-MS. The predominant volatile compounds were α-pinene, sabinene, limonene, and β-phellandrene (more than 60% of the oil). The β-pinene, β-myrcene, camphene, β-caryophyllene, bornyl acetate, and germacrene D content was also high. The in-vitro antibacterial activity of the essential oil and some of its components against ten reference strains of Gram-positive and Gram-negative bacteria was evaluated by the agar dilution method. The essential oil and the compounds were more active against Gram-positive than Gram-negative bacteria.
Phytochemistry Reviews, 2014
Natural products extracts are commonly highly complex mixtures of active compounds and consequent... more Natural products extracts are commonly highly complex mixtures of active compounds and consequently their purification becomes a particularly challenging task. The development of a purification protocol to extract a single active component from the many hundreds that are often present in the mixture is something that can take months or even years to achieve, thus it is important for the natural product chemist to have, at their disposal, a broad range of diverse purification techniques. Counter-current chromatography (CCC) is one such separation technique utilising two immiscible phases, one as the stationary phase (retained in a spinning coil by centrifugal forces) and the second as the mobile phase. The method benefits from a number of advantages when compared with the more traditional liquid-solid separation methods, such as no irreversible adsorption, total recovery of the injected sample, minimal tailing of peaks, low risk of sample denaturation, the ability to accept particulates, and a low solvent consumption.
Journal of Separation Science, 2012
A high-performance counter-current chromatography (HPCCC) method was applied for the first time f... more A high-performance counter-current chromatography (HPCCC) method was applied for the first time for the preparative separation and purification of three rare compounds which occur as minor constituents in the fruits of Peucedanum alsaticum L.: 5-substituted coumarin notoptol and two dihydropyranochromones: divaricatol and ledebouriellol. A scale-up process from analytical to preparative in a very short time was developed. In order to purify a range of rare and minor compounds with different polarity two separate experiments were performed, one in reverse phase, the other in normal phase, using the same crude extract. A two-phase solvent system composed of n-hexane/ethyl acetate/methanol/water (1:1:1:1) was developed. The components purified and collected were analyzed by high-performance liquid chromatography. The method yielded 0.7 mg of notoptol, 1.46 mg of ledebouriellol at purity of 99.5%, and 10 mg of mixtures of divaricatol, alsaticol and alsaticocoumarin, where divaricatol present 22% by peak area. These amounts were obtained from 1 g of the crude extract in a single run. This is the first time when minor notoptol, ledebouriellol, and divaricatol were isolated in a single run using HPCCC method and first time when these were identified in plant from Peucedanum genus.
Journal of Chromatography A, 2009
A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for ... more A preparative high-speed counter-current chromatography (HSCCC) method was successfully used for isolation of two new minor compounds -alsaticol and alsaticocoumarin A. A two-phase solvent system composed of n-hexane-ethyl acetate-methanol-water (1:1:1:1) was developed. Compounds were obtained from the dichloromethane extract of Peucedanum alsaticum fruits and their identification was performed with NMR and MS methods. Optimized HSCCC offers a rapid method of obtaining new natural compounds.
Imperatorin, a psoralen-type furanocoumarin, is a potent myorelaxant agent acting as a calcium an... more Imperatorin, a psoralen-type furanocoumarin, is a potent myorelaxant agent acting as a calcium antagonist on vascular smooth muscle. Its effects on other types of smooth muscle remain unknown. Therefore, the aim of this study was to investigate the hypothesized myorelaxant effect of imperatorin on gut motor activity and, possibly, to define the underlying mechanism of action. Imperatorin was made available for pharmacological studies from the fruits of the widely available Angelica officinalis through the application of high-performance countercurrent chromatography (HPCCC). Imperatorin generated reversible relaxation of jejunum strips dose-dependently (1-100 M). At 25 and 50 M, imperatorin caused relaxation comparable to the strength of the reaction induced by isoproterenol (Isop) at 0.1 M. The observed response resulted neither from the activation of soluble guanylate cyclase, nor from -adrenoreceptor involvement, nor from Ca 2+ -activated potassium channels. Imperatorin relaxed intestine strips precontracted with high potassium concentration, attenuated the force and duration of K + -induced contractions, and modulated the response of jejunum strips to acetylcholine. The results suggest that imperatorin probably interacts with various Ca 2+ influx pathways in intestine smooth muscle. The types of some calcium channels involved in the activity of imperatorin will be examined in a subsequent study.
Industrial Crops and Products
Ficus drupacea is a widely distributed species with ethnopharmacological applications. Despite th... more Ficus drupacea is a widely distributed species with ethnopharmacological applications. Despite the large body of literature on its antioxidant and phenolic contents, several aspects of its biological activities remain unexplored. In this study, the antimicrobial (fungi and bacteria) and antiproliferative activities of crude extracts of the plant’s stem bark and isolated compounds were investigated. Seven biochemical compounds from stem bark extracts including β-amyrin (1), β-sitosterol-3-O-β-D-glucopyranoside (2), 5-O-methyllatifolin (3), oleanolic acid (4), epifriedelanol (5), friedelin (6) and epilupeol acetate (7) were isolated and identified. Of all the seven compounds, the compounds 3 and 7 exhibited the highest antifungal and antibacterial activities against screened microorganisms. Aspergillus versicolor and A. ochraceus were the most sensitive microorganisms to the isolated compounds whereas Candida albicans was the most resistant fungus. Compounds 4, 5, and 6 did not exhibi...
Evidence-Based Complementary and Alternative Medicine, 2015
Imperatorin, a psoralen-type furanocoumarin, is a potent myorelaxant agent acting as a calcium an... more Imperatorin, a psoralen-type furanocoumarin, is a potent myorelaxant agent acting as a calcium antagonist on vascular smooth muscle. Its effects on other types of smooth muscle remain unknown. Therefore, the aim of this study was to investigate the hypothesized myorelaxant effect of imperatorin on gut motor activity and, possibly, to define the underlying mechanism of action. Imperatorin was made available for pharmacological studies from the fruits of the widely available Angelica officinalis through the application of high-performance countercurrent chromatography (HPCCC). Imperatorin generated reversible relaxation of jejunum strips dose-dependently (1-100 μM). At 25 and 50 μM, imperatorin caused relaxation comparable to the strength of the reaction induced by isoproterenol (Isop) at 0.1 μM. The observed response resulted neither from the activation of soluble guanylate cyclase, nor from β-adrenoreceptor involvement, nor from Ca(2+)-activated potassium channels. Imperatorin relaxed intestine strips precontracted with high potassium concentration, attenuated the force and duration of K(+)-induced contractions, and modulated the response of jejunum strips to acetylcholine. The results suggest that imperatorin probably interacts with various Ca(2+) influx pathways in intestine smooth muscle. The types of some calcium channels involved in the activity of imperatorin will be examined in a subsequent study.
Pharmaceutical Biology, 2015
Plants of the genus Heracleum L. (Apiaceae) have a long history of being used in traditional medi... more Plants of the genus Heracleum L. (Apiaceae) have a long history of being used in traditional medicines for the treatment of alimentary tract disorders, and these biological effects have been ascribed to the presence of furanocoumarins (including bergapten). This study aimed to develop an efficient, preparative, counter-current chromatographic separation of bergapten in order to characterize its spasmolytic activity in isolated rat jejunum strips. Successful separation of the dichloromethane extract of the fruits of Heracleum leskovii Grossh. was achieved by high-performance countercurrent chromatography (HPCCC) using a two-phase solvent system composed of n-heptane/EtOAc/MeOH/H2O (6:5:6:5, v/v/v/v). The pharmacological assessment of bergapten (0.0001-50 μM) on jejunum smooth muscle strips isolated from rats was conducted under isotonic conditions, following up to three hours of incubation. The separation method was scaled up six-fold from analytical to semi-preparative conditions, affording bergapten of >99% purity in less than 30 min. This permitted bergapten to be available in quantity for spasmolytic tests on isolated jejunum strips from rats. Bergapten caused myorelaxation of the intestine preparations in the concentration range of 0.0001-1 μM. At higher doses, bergapten caused either relaxation or contraction of the smooth muscle. Bergapten was successfully isolated by rapid HPCCC and its spasmolytic activity was confirmed, thereby providing a preliminary evidence base for the traditional medicine application. The data suggest that bergapten causes no irreversible changes to intestinal tissue.
Phytochemistry Reviews, 2014
Natural products extracts are commonly highly complex mixtures of active compounds and consequent... more Natural products extracts are commonly highly complex mixtures of active compounds and consequently their purification becomes a particularly challenging task. The development of a purification protocol to extract a single active component from the many hundreds that are often present in the mixture is something that can take months or even years to achieve, thus it is important for the natural product chemist to have, at their disposal, a broad range of diverse purification techniques. Counter-current chromatography (CCC) is one such separation technique utilising two immiscible phases, one as the stationary phase (retained in a spinning coil by centrifugal forces) and the second as the mobile phase. The method benefits from a number of advantages when compared with the more traditional liquid-solid separation methods, such as no irreversible adsorption, total recovery of the injected sample, minimal tailing of peaks, low risk of sample denaturation, the ability to accept particulates, and a low solvent consumption. The selection of an appropriate two-phase solvent system is critical to the running of CCC since this is both the mobile and the stationary phase of the system. However, this is also by far the most time consuming aspect of the technique and the one that most inhibits its general take-up. In recent years, numerous natural product purifications have been published using CCC from almost every country across the globe. Many of these papers are devoted to terpenoids-one of the most diverse groups. Naturally occurring terpenoids provide opportunities to discover new drugs but many of them are available at very low levels in nature and a huge number of them still remain unexplored. The collective knowledge on performing successful CCC separations of terpenoids has been gathered and reviewed by the authors, in order to create a comprehensive document that will be of great assistance in performing future purifications.
Food Chemistry, 2009
The aim of this paper was to evaluate the antioxidant properties of the different extracts from L... more The aim of this paper was to evaluate the antioxidant properties of the different extracts from Lycopus lucidus (crude methanolic extract, dichloromethane, diethyl ether and ethyl acetate sub-fractions) and to correlate their antioxidant potential to the composition of polyphenols. The ability of deactivating free radicals was measured using Å DPPH and Å NO scavenging assays. The reducing power towards transition metals was tested by the phosphomolybdenum method. Qualitative and quantitative analyses of major phenolic acids were also performed. The main antioxidant compound from the methanol extract was confirmed to be rosmarinic acid. Other identified phenolic acids that are likely to contribute to the antioxidant potential are: ferulic, caffeic, chlorogenic, vanillic, p-hydroxybenzoic acid, p-coumaric acid and protocatechuic acid. The most active ethyl acetate extract contained the highest level of rosmarinic acid, but the luteolin glycosides are also the major determinants of the antioxidant activity as has been revealed by analysis of the two most active fractions from column chromatography. These two classes of antioxidants are likely to act synergistically in the ethyl acetate fraction and the crude extract of the plant.
Brain Research Bulletin, 2015
According to the World Health Organization, two billion people will be aged 60 years or older by ... more According to the World Health Organization, two billion people will be aged 60 years or older by 2050. Aging is a major risk factor for a number of neurodegenerative disorders. These age-related disorders currently represent one of the most important and challenging health problems worldwide. Therefore, much attention has been directed towards the design and development of neuroprotective agents derived from natural sources. These phytochemicals have demonstrated high efficacy and low adverse effects in multiple in vitro and in vivo studies. Among these phytochemicals, dietary flavonoids are an important and common chemical class of bioactive products, found in several fruits and vegetables. Luteolin is an important flavone, which is found in several plant products, including broccoli, pepper, thyme, and celery. Numerous studies have shown that luteolin possesses beneficial neuroprotective effects both in vitro and in vivo. Despite this, an overview of the neuroprotective effects of luteolin has not yet been accomplished. Therefore, the aim of this paper is to provide a review of the available literature regarding the neuroprotective effects of luteolin and its molecular mechanisms of action. Herein, we also review the available literature regarding the chemistry of luteolin, its herbal sources, and bioavailability as a pharmacological agent for the treatment and management of age-related neurodegenerative disorders.
Postępy Higieny i Medycyny Doświadczalnej (Advances in Hygiene and Experimental Medicine)
Mimo znaczącego postępu w medycynie i farmacji w ostatnich dekadach tradycyjne strategie terapeut... more Mimo znaczącego postępu w medycynie i farmacji w ostatnich dekadach tradycyjne strategie terapeutyczne stosowane wterapii chorób bakteryjnych czy wirusowych są często niezadowalające iwywołują wiele działań niepożądanych. W związku z tym obecnie obserwuje się ponowny wzrost zainteresowania substancjami pochodzenia roślinnego jako potencjalnymi, obiecującymi terapeutykami w profilaktyce i leczeniu wielu chorób, w tym chorób infekcyjnych. Terpeny oraz ich pochodne stanowią ogromną grupę naturalnych związków organicznych, które wchodzą wskład olejków eterycznych i są szeroko rozpowszechnione w królestwie roślin. Liczne badania potwier-dzają, że olejki eteryczne oraz ich terpenowe składniki wykazują szeroki zakres działania biologicznego i farmakologicznego w warunkach in vitro. Udowodniono, że działają one przeciwbakteryjnie, przeciwwirusowo, przeciwgrzybiczo i przeciwpasożytniczo. Ponadto przejawiają aktywność przeciwzapalną oraz stymulują układ immunologiczny. Szeroki zakres właściwo...
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Papers by Krystyna Skalicka-woźniak