Octospora neerlandica'nın Asya Kıtasından İlk Kaydı

MANTAR DERGİSİ/The Journal of Fungus Geliş(Recevied) Kabul(Accepted) Nisan(2021)12(1)61-64 :10.12.2020 :28.01.2021 Research Article Doi: 10.30708.mantar.838701 First Report of Octospora neerlandica from Asian Continent Osman BERBER1, Yasin UZUN2* Abdullah KAYA3 *Sorumlu yazar: yuclathrus@gmail.com 1Karaman Provincial Directorate of Agriculture and Forestry, 70100, Karaman, Turkey Orcid ID: 0000-0002-0265-4441 / ober70@hotmail.com 2Karamanoğlu Mehmetbey University, Ermenek Uysal & Hasan Kalan Health Services Vocational School, 70400, Karaman, Turkey Orcid ID:0000-0002-6423-6085 / yuclathrus@gmail.com 3Gazi University, Science Faculty, Department of Biology, 06560 Ankara, Turkey Orcid ID: 0000-0002-4654-1406 / kayaabd@hotmail.com Abstract: The bryophillic ascomycete species, Octospora neerlandica Benkert & Brouwer, is reported as a new record from Turkey, based on the identification of the samples collected from Niğde province. A brief description and photographs, related to the macroscopy and microscopy of the species, are provided. Key words: Biodiversity, bryophillic ascomycete, new record, Pyronemataceae, Turkey Octospora neerlandica'nın Asya Kıtasından İlk Kaydı Öz: Briyofilik askomiset türü olan, Octospora neerlandica Benkert & Brouwer, Niğde’den toplanan örneklerin teşhis edilmesiyle, Türkiye’den yeni kayıt olarak rapor edilmiştir. Türün kısa bir betimlemesi ve makroskobi ve mikroskobisine ilişkin fotoğrafları verilmiştir. Anahtar kelimeler: Biyoçeşitlilik, briyofilik askomiset, yeni kayıt, Pyronemataceae, Türkiye Introduction Octospora Hedw is an ascomycete genus within the family Pyronemataceae Corda. Moss associated apothecia, typical marginal hyphae, and ellipsoid to globose or rounded, sometimes ornamented, guttulate spores generally characterize the genus (Yao and Spooner, 1996). Kirk et al. (2008) reports the presence of 84 species of the genus, eighty one of which also exist in Europe (Benkert, 2007). Octospora itzerottii Benkert and O. leucoloma Hedw. were the first two Octospora species reported in Turkey (Çolak and Kaygusuz, 2017; Uzun et al., 2017). In the following two years, eleven members of the genus, O. areolata (Seaver) Caillet & Moyne, O. axillaris (Nees) M.M. Moser, O. coccinea (P. Crouan & H. Crouan) Brumm., O. excipulata (Clem.) Benkert, O. gemmicola Benkert, O. grimmiae Dennis & Itzerott, O. lilacina (Seaver) Svrček & Kubička, O. musci-muralis Graddon, O. orthotrichi (Cooke & Ellis) K.B. Khare & V.P. Tewari, O. polytrichi (Schumach.) Caillet & Moyne and O. rustica (Velen.) J.Moravec were also presented as new records for the mycobiota of Turkey by Uzun et al. (2018) and Uzun and Kaya (2019). But the current checklists (Sesli and Denchev, 2014; Solak et al., 2015) on Turkish macromycota and the later contributions (Alkan et al., 2018; Doğan et al., 2018; Işık and Türkekul, 2018; Acar et al., 2019; Allı et al., 2019; Berber et al., 2019; Keleş, 2019; Şelem et al., 2019; Türkekul and Işık, 2019; Akçay, 2020; Çağli and Öztürk, 2020; Çelik et al., 2020; İleri et al., 2020; Sadullahoğlu and Uzun, 2020; Sesli, 2020; Uzun et al., 2020), indicate that, O. neerlandica hasn’t been reported from Turkey before. The study aims to make a contribution to the mycobiota of Turkey. Material and method The fruit body of O. neerlandica was collected from Ulukışla district of Niğde province, in 2018, during a routine field trip performed to determine the macrofungal biodiversity of the district. The sample was photographed at its natural habitat, and ecological characteristics and geographic position were noted. Then it was put in a 61 MANTAR DERGİSİ/The Journal of Fungus paper box and transferred to the fungarium where it was dried and prepared as fungarium material. Microscopic investigations were carried out on dry samples. A Nikon Eclipse Ci-S trinocular microscope was used for microscopic investigation and a DS-Fi2 digital camera was used to get microstructural photographs. The sample was identified with the help of Benkert and Brouwer (2004). The specimen is kept at Karamanoğlu Mehmetbey University, Kâmil Özdağ Science Faculty, Department of Biology. Results Ascomycota Caval-Sm Pezizomycetes O.E.Erikss. & Winka Pezizales J.Schröt. Nisan(2021)12(1)61-64 Pyronemataceae Corda Octospora neerlandica Benkert & Brouwer, Persoonia 18(3): 381 (2004) Macroscopic and microscopic features: Apothecium 1.8 mm, margin fimbriate, membranaceous, hymenial surface pinkish-orange to pale orange (Figure 1a), outside portion somewhat paler. Asci 210-290  1318 μm, cylindrical, 8-spored, spores uniseriate Paraphyses slender, generally straight, some slightly curved, enlarged up to 3-7 μm, at the apex (Figure 1b). Ascopores 16-18  11-12.8 μm, ellipsoid, one oil drop generally accompained by smaller droplets, spore ornamentation constists of an irregular reticulum with variably formed meshes (Figure 1c). Figure 1. Ascocarp (a), asci and paraphyses (b) and ascospores (c) of Octospora neerlandica (bars: a- 1 mm, b,c- 10 μm) 62 MANTAR DERGİSİ/The Journal of Fungus Octospora neerlandica was reported to grow on sandy soil among or directly on the the stems of the mosses Tortula ruralis (Hedw.) Gaertn., Meyer, & Scherb., T. ruraliformis (Besch.) Ingham or T. virescens (De Not.) De Not. (Benkert and Brouwer, 2004). Specimen examined: Turkey, Niğde, Ulukışla, Çiftehan village, on soil among mosses under Pinus sp., 37°30′N, 34°47′E, 930 m, 07.01.2018, O.Ber-108. Discussion Octospora neerlandica is reported for the first time for the mycobiota of Turkey. It is the 14th member of the genus Octospora in Turkey (Çolak and Kaygusuz, 2017; Uzun et al., 2017, 2018; Uzun and Kaya, 2019). Macroscopic, microscopic and habitat characteristics of Turkish collection are in agreement with Benkert and Brouwer (2004). Nisan(2021)12(1)61-64 Due to their morphological similarities and very small size, Octospora species can easily be overlooked and it is very hard to separate their species from each other morphologically (Itzerott, 1977). But the spore characteristics and substrate preferation of O. neerlandica makes it a very distinctive species. The spore ornamentation of this species constists of an irregular reticulum with variably formed meshes. Though Lamprospora seaveri Benkert has a remarkable similar spore ornamentation, it has globose spores and is associated with other moss species. Likewise very little is known about the infection of the memsers of the moss genus Tortula Hedw. by Octospora species. Although O. crosslandii (Dennis & Itzerott) Benkert, has been observed in association with Tortula species together with many other moss species, the globose spores of this species easily differentiates it from O. neerlandica (Benkert and Brouwer, 2004). References Acar, İ., Uzun, Y., Keleş, A. and Dizkırıcı Tekpınar, A. (2019). Suillellus amygdalinus, a new species record for Turkey from Hakkari Province. Anatolian Journal of Botany, 3(1): 25-27. Akçay, M.E. (2020). A new record for the Mycota of Turkey. Anatolian Journal of Botany, 4(1): 8-10. Alkan, S., Aktaş, S. and Kaşık, G. (2018). Tuber species in Turkey and a new locality for Tuber aestivum. Selcuk University Journal of Science Faculty, 44(1): 25-29. Allı, H., Tevlim, G. and Şen, İ. (2019). A new record for Turkey's mycobiota from an interesting habitat in the Muğla province: Hortiboletus bubalinus (Oolbekk. & Duin) L. Albert & Dima. Muğla Journal of Science and Technology, 5(1): 114-118. Benkert, D. (2007). Zur Kenntnis des Vorkommens bryophiler Pezizales (Ascomycota) in Südost-Europa. Mycologia Montenegrina, 10: 7-21. Benkert, D. and Brouwer, E. (2004). New species of Octospora and some further remarkable bryoparasitic Pezizales from the Netherlands. Persoonia, 18(3): 381-391. Berber, O., Uzun, Y. and Kaya, A. (2019). Genea lobulata, a new hypogeous ascomycete record for Turkish Mycobiota. Süleyman Demirel University Journal of Natural and Applied Sciences, 23(3): 922-924. Çağli, G. and Öztürk, A. (2020). Macromycetes determined in Muradiye (Van) district. Anatolian Journal of Botany, 4(1): 57-64. Çelik, A., Uzun, Y. and Kaya, A. (2020). Macrofungal biodiversity of Güneysınır district (Konya-Turkey). The Journal of Fungus, 11(1): 75-83. Çolak, Ö.F. and Kaygusuz, O. (2017). Octospora leucoloma (Pyronemataceae): a new bryoparasitic genus record for Turkish mycobiota. Phytologia Balcanica, 23(3): 345-348. Doğan, H.H., Bozok, F. and Taşkın, H. (2018). A new species of Barssia (Ascomycota, Helvellaceae) from Turkey. Turkish Journal of Botany, 42(5): 636-643. Işık, H. and Türkekul, İ. (2018). New additions to Turkish macrofungi from Tokat and Yozgat Provinces. Mycotaxon, 133(4): 697-709. Itzerott, H. (1977). The genus Octospora and an attempt to solve its taxonomic problems. Kew Bulletion, 31(3): 497-500. İleri, R., Uzun, Y. and Kaya, A. (2020). Macromycetes of Karadağ (Karaman) and its environs. The Journal of Fungus, 11(1): 57-63. Keleş, A. (2019). New records of macrofungi from Trabzon province (Turkey). Applied Ecology and Environmental Research, 17(1): 1061-1069. Kirk, P.M., Cannon, P.F., Minter, D.W. and Stalpers, J.A. (2008). Dictionary of the Fungi, 10th ed. CAB International, Wallingford. Sadullahoğlu, C. and Uzun, Y. (2020). Karz Dağı (Tatvan-Bitlis) ve çevresinde belirlenen makrofunguslar. Mantar Dergisi, 11(1): 1-11. Sesli, E. (2020). Clitopilus cystidiatus (Entolomataceae), Türkiye Mikotası için yeni bir kayıt. Mantar Dergisi, 11(1): 90-93. 63 MANTAR DERGİSİ/The Journal of Fungus Nisan(2021)12(1)61-64 Sesli, E. and Denchev, C.M. (2014). Checklists of the myxomycetes, larger ascomycetes, and larger basidiomycetes in Turkey. 6th edn. Mycotaxon Checklists Online (http://www.mycotaxon.com/resources/checklists/sesli-v106checklist.pdf): 1-136. Solak, M.H., Işıloğlu, M., Kalmış, E. and Allı, H. (2015). Macrofungi of Turkey, Checklist. Volume- II. Üniversiteliler Ofset, İzmir. Şelem, E., Keleş, A., Acar, İ. and Demirel, K. (2019). Edible macrofungi determined in Gürpınar (Van) district. Anatolian Journal of Botany, 3(1): 7-12. Türkekul, İ. and Işık, H. (2019). Macrofungal biodiversity of Reşadiye (Tokat) district. Acta Biologica Turcica, 32(2): 95-101. Uzun, Y. and Kaya, A. (2019). New Additions to Turkish Pezizales from East Blacksea Region. Turkish Journal of Botany, 43(2): 262-270. Uzun, Y., Acar, İ., Akçay, M.E. and Sadullahoğlu, C. (2020). Kağızman (Kars) yöresi makrofungusları. Mantar Dergisi, 11(1): 19-28. Uzun, Y., Karacan, İ.H., Yakar, S. and Kaya, A. (2017). Octospora Hedw., A New Genus Record for Turkish Pyronemataceae. Anatolian Journal of Botany, 1(1): 18-20. Uzun, Y., Karacan, İ.H., Yakar, S. and Kaya, A. (2018). New bryophillic Pyronemataceae records for Turkish Pezizales from Gaziantep province. Anatolian Journal of Botany, 2(1): 28-38. Yao, Y.J. and Spooner, B.M. (1996). Notes on British species of Octospora. Mycological Research, 100: 175-178. 64