Documentation
Summary
- EMDB data model
- EMDB header data model
- Policies
- Search engine
- Chart builder
- FAQ
- Deposition
- Segmentation data model prototype
EMDB map data model
The EM Data Bank (EMDB) accepts and distributes 3D map volumes derived from several types of EM reconstruction methods, including single particle averaging, helical averaging, 2D crystallography, and tomography. Since its inception in 2002, the EMDB map distribution format has followed CCP4 definition (CCP4 map format) , which is widely recognized by software packages used by the structural biology community. CCP4 map format is closely related to the MRC map format used in the 3DEM community (MRC map format); CCP4 is slightly more restrictive, in that voxel positions are limited to a grid that includes the Cartesian coordinate origin (0,0,0). Further details can be found here.
EMDB header data model
Every EMDB entry has a header file containing meta data (e.g., sample, detector, microscope, image processing) describing the experiment. The header file is an XML file and the structure and content of the header file is described by a XSD data model. With a highly dynamic field such as cryo-EM there is a constant need to adapt and modify the schema to keep it up-to-date with the most recent developments. We consult extensively with the EM community regarding such issues and version the schema according to the policy described here.
Data model version 1.9
This has been a long-term stable version of the data model. It was be replaced in 2018 with an updated model but XML header files in version 1.9 continues to be distributed in parallel for at least one year to give EMDB users ample time to switch. It should be noted that the generation of the version 1.9 header files will be on a best effort basis but involves a back translation from recent versions that are richer in content and will therefore not contain all the information that can be found in the more recent versions.
Download schema
Browse schema documentation
Download Python code to facilitate reading and writing XML version 1.9 header files
Data model version 3.0 (current model)
This data model replaced version 1.9, however header files corresponding to both data models will be distributed in parallel with the view of stopping the distribution of the version 1.9 files in 2019 once users have had a chance to adopt version 3.0.
This version adds a number of features including:
- An improved description of direct electron detectors, specimen preparation and tomography experiments.
- A hierarchal description of the overall sample composition in combination with a low-level description of the macromolecular composition to allow the description of both molecular and cellular samples.
- Specific data items describing the half-maps and segmentations included with the entry.
Download schema
Browse schema documentation
Download Python code to facilitate reading and writing XML version 1.9 header files
Segmentation data model prototype
Find out more about the project here.
Publications
- Patwardhan, Ardan, Robert Brandt, Sarah J. Butcher, Lucy Collinson, David Gault, Kay Grünewald, Corey Hecksel et al. Building bridges between cellular and molecular structural biology. eLife 6 (2017).
- Patwardhan, Ardan, Alun Ashton, Robert Brandt, Sarah Butcher, Raffaella Carzaniga, Wah Chiu, Lucy Collinson et al. A 3D cellular context for the macromolecular world. Nature structural & molecular biology 21, no. 10 (2014): 841-845.
- Patwardhan, Ardan, José-Maria Carazo, Bridget Carragher, Richard Henderson, J. Bernard Heymann, Emma Hill, Grant J. Jensen et al. Data management challenges in three-dimensional EM. Nature structural & molecular biology 19, no. 12 (2012): 1203-1207.
Quick links
Recent Entries
(Show all)Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-9 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
Structure of Lassa virus glycoprotein (Josiah) on the surface of VSVdG-Lassa-GPC vaccine particle.
Cryo-EM structure of Maackia amurensis seed Leukoagglutinin (lectin), MASL
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-8 at Day 26
nsEM map of macaque 32653 week 10 polyclonal Fab in complex with BG505 v5.2 Prime immunogen
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-9 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-13 at Day 26
Polyclonal immune complex of Fab from pooled Ferret sera at day 28 binding B/Washington NA after infection with B/Washington/02/2019
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-11 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26
nsEM map of macaque 32653 week 18 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-9 at Day 26
AlphaIIbbeta3 in fully-extended conformation in complex with R6H8 Fab
nsEM map of macaque 32647 week 10 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
In situ cryoET of an EPI vesicle in a Goslar infected chmA KD E. coli cell 30 mpi
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-9 at Day 26
Polyclonal immune complex of Fab from pooled Ferret sera at day 28 binding B/Washington HA after infection with B/Washington/02/2019
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26
Cryo-EM structure of the Measles Virus polymerase (L) protein in complex with the tetrameric phosphoprotein (P)
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-9 at Day 26
nsEM map of macaque 32653 week 14 polyclonal Fab in complex with BG505 v5.2 Prime immunogen
nsEM map of macaque 32653 week 6 polyclonal Fab in complex with BG505 v5.2 Prime immunogen
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-9 at Day 26
Cryo-EM structure of the Measles Virus polymerase (L) protein in complex with the tetrameric phosphoprotein (P) and C protein
Polyclonal Immune Complex of Fab binding the Lassa Virus Glycoprotein from serum of Subject-5 at Day 26
Nipah virus fusion glycoprotein in complex with a broadly neutralizing antibody 5C8
The chimeric flagellar motor complex between MotA1B1 from Paenibacillus sp. TCA20 and MotAB from E.coli, state 3
Cryo-EM structure of nanodisc-reconstituted wildtype human MRP4 (apo-form)
The chimeric flagellar motor complex between MotA1B1 from Paenibacillus sp. TCA20 and MotAB from E.coli, state 1
Cryo-EM structure of Neuropeptide FF receptor 2 in complex with hNPSF and Gi
Cryo-EM structure of nanodisc-reconstituted human MRP4 withE1202Q mutation (in complex with lapatinib)
Cryo-EM structure of nanodisc-reconstituted wildtype human MRP4 (in complex with vincristine)
Cryo-EM structure of nanodisc-reconstituted human MRP4 withE1202Q mutation (in complex with 5-Fluorouracil)
Nipah virus fusion glycoprotein in complex with a broadly neutralizing antibody 1D6
Cryo-EM structure of neuropeptide FF receptor 2 in the ligand-free state with BRIL fusion, anti-BRIL Fab, and nanobody
The chimeric flagellar motor complex between MotA1B1 from Paenibacillus sp. TCA20 and MotAB from E.coli, state 2
Structure of nucleosome of isw1-nucleosome double-bound complex in ADP-ADP+ state
Cryo-EM structure of the RNA-dependent RNA polymerase complex from Marburg virus
Cryo-EM structure of OXGR1 bound to alpha-ketoglutarate and Gq proteins
Cryo-EM structure of the CHIKV nsP3 peptide in complex with the NTF2L domain of G3BP1 (Conformation III)
Cryo-EM structure of the CHIKV nsP3 peptide in complex with the NTF2L domain of G3BP1 (Conformation II)
Cryo-EM structure of the CHIKV nsP3 peptide in complex with the NTF2L domain of G3BP1 (Conformation I)
Structure of motor2 of isw1-nucleosome double-bound complex in ADP-ADP+ state
Cryo-EM structure of OXGR1 bound to leukotriene E4 and Gq proteins
Cryo-EM structure of neurotensin receptor 1 in complex with beta-arrestin1 and SBI-553 (complex 3)
Cryo-EM structure of neurotensin receptor 1 in complex with beta-arrestin1 and SBI-553 (complex2)
Cryo-EM structure of the RNA-dependent RNA polymerase complex in a compact conformation from Ebola virus
Structure motor1 of isw1-nucleosome double-bound complex in ATP-ATP state
Structure of motor2 of isw1-nucleosome double-bound complex in ATP-ATP state
Structure of nucleosome of isw1-nucleosome double-bound complex in ATP-ATP state
Structure of isw1-nucleosome double-bound complex in ADP-ADP+ state
Cryo-EM structure of neurotensin receptor 1 in complex with beta-arrestin 1
Cryo-EM structure of the RNA-dependent RNA polymerase complex from Marburg virus
Structure of isw1-nucleosome double-bound complex in ATP-ATP state
Lysosomal transporting complex of beta-glucocerebrosidase (GCase) and lysosomal integral membrane protein 2 (LIMP-2) with bound Pro-macrobodies (LIMP-2 local refinement)
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 central spike protein - knob and petal domains
Cryo-EM structure of E. coli transcription factor NrdR in complex with DNA
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - complete
Structure of the BMOE-crosslinked transcription termination factor Rho in the presence of ppGpp; S84C/M405C double mutant
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, inner tripod, arm segment, and proximal tail sheath proteins
ADP:BeF3-bound human mitochondrial Hsp60-Hsp10 half-football complex
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - complete
Tail tube and extended tail sheath tube of Klebsiella phage KP1 variant vB_Kpn_Lilla1
Cryo-EM structure of KBTBD4 WT-HDAC2 2:2 complex mediated by molecular glue UM171
CryoEM structure of bacterial transcription intermediate complex mediated by activator PspF containing nifH promoter DNA containing mismatch from -11 to -8 - conformation 3
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the post-contraction state - sheath initiator, wedge module, and inner tripod proteins
3DFlex refinement of the CryoEM structure of DeCLIC nanodisc with 10mM EDTA in sym-like state
Lysosomal transporting complex of beta-glucocerebrosidase (GCase) and lysosomal integral membrane protein 2 (LIMP-2) with bound Pro-macrobodies (Combined focus map)
Cryo-EM structure of KBTBD4 WT-HDAC2 2:1 complex mediated by molecular glue UM171
Lysosomal transporting complex of beta-glucocerebrosidase (GCase) and lysosomal integral membrane protein 2 (LIMP-2) with bound Pro-macrobodies (consensus map)
Lysosomal transporting complex of beta-glucocerebrosidase (GCase) and lysosomal integral membrane protein 2 (LIMP-2) with bound Pro-macrobodies (GCase local refinement map)
auxin transporter PIN8 as asymmetric dimer (inward/outward) with 2,4-D bound in the inward vestibule prebinding state
Non-uniform refinement of the CryoEM structure of DeCLIC nanodisc with 10mM EDTA in asym state
Non-uniform refinement of the CryoEM structure of DeCLIC nanodisc with 10mM calcium
auxin transporter PIN8 as asymmetric dimer (outward/inward) with 4-CPA bound in the outward partly released state
auxin transporter PIN8 as symmetric dimer (outward/outward) with 4-CPA bound in the outward binding state
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - core, wedge module, and proximal tail proteins
3DFlex refinement of the CryoEM structure of DeCLIC nanodisc with 10mM calcium
Cryo-EM map of KBTBD4 WT-HDAC2-CoREST1 2:1:1 complex mediated by molecular glue UM171
3DFlex refinement of the CryoEM structure of DeCLIC nanodisc with 10mM EDTA in asym state
auxin transporter PIN8 as asymmetric dimer (inward/outward) with 4-CPA bound in the inward vestibule prebinding state
Non-uniform refinement of the CryoEM structure of DeCLIC nanodisc with 10mM EDTA in sym-like state
Cryo-EM structure of Staphylococcus aureus bacteriophage phi812 baseplate in the pre-contraction state - core, and wedge module proteins
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
In situ cryoET of an EPI vesicle in a Goslar infected chmA KD E. coli cell 90 mpi
nsEM map of macaque 32647 week 14 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-11 at Day 26
Fab1-1 in complex with the capsid of Adeno-associated virus type 9
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-8 at Day 26
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-7 at Day 26
nsEM map of macaque 32647 week 22 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26
In situ cryoET of an EPI vesicle in a Goslar infected chmA KD E. coli cell 90 mpi
nsEM map of macaque 32653 week 26 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
nsEM map of macaque 32653 week 22 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
nsEM map of macaque 32647 week 18 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
nsEM map of macaque 32653 week 42 polyclonal Fab in complex with BG505 v5.2 Boost #2 immunogen
In situ cryoET of an EPI vesicle in a Goslar infected WT E. coli cell 1 mpi
Immune complex of Lassa virus glycoprotein (Josiah) with polyclonal Fab from Subject-12 at Day 26