Disney has donated $1 million to the OneOrlando fund, which was set up to support the victims of the tragic Orlando club shootings.
The company's pledge will help to support the families of the 49 victims, and aid the recovery of the 53 injured in the attack.
Late on Saturday night, gunman Omar Mateen opened fire in a gay club in Florida in the worst mass shooting in recent US history.
Disney has also announced that it will match pledges from individual members of staff.
Bob Chapek, chairman of Walt Disney Parks and Resorts, released a statement saying: "We are heartbroken by this tragedy and hope our commitment will help those in the community affected by this senseless act.
"With 74,000 cast members who call Orlando home, we mourn the loss of the victims and offer our condolences to their families, friends and loved ones."
The world is still in shock over the attack, with many taking a moment to pay tribute to the victims on social media.
Meanwhile, Owen Jones - author and columnist for The Guardian - stormed out of a live segment on Sky News during a very heated discussion on the motivations behind the attack.
