Uranyl nitrate hexahydrate was procured from Honeywell Fluka.
Muscle samples were washed in 0.1 M sodium cacodylate buffer, post-fixed in 1% Os[O.sub.4] for 2 h at room temperature, washed again in the same buffer followed by distilled water, contrasted with 5% aqueous
uranyl acetate solution for 1 h and finally washed in distilled water (Rocha, Leonardo, De Souza, Palma, Da Cruz-Hofling, 2008; Rocha, Souza, Palma, da Cruz-Hofling, & Harris, 2009).
A known amount of
uranyl nitrate was added into it and mixed thoroughly and then 1mL of this solution was pipetted out in a 50 mL pyrex glass beaker as reference before extraction.
The TEM of 100-nm thin sections stained after fixation with
uranyl acetate and lead citrate revealed numerous membrane-bound envelopes, some of which appeared as empty voids, approximately 500 nm across and 100-200 nm in height, near the BL (Fig.
Noticeable exceptions include
uranyl chloride complexes, where we use the new properties obtained recently by Migdisov et al.
Sections were stained with
uranyl acetate and lead citrate, and examined.
District Court for the District of Columbia, found that the federal Department of Energy met all the requirements to allow subcontracted flatbed tractor-trailers to haul a total of 6,000 gallons of "target material"--called
uranyl nitrate--from a laboratory in Ontario, Canada, to a federal processing plant in South Carolina.
In recent years,
uranyl complexes have attracted much attention for exploring reactivity and coordination behavior of 5f-elements, developing suitable trivalent actinide (An(III)) extractants for nuclear remediation [1], screening highly effective chelators structure for decorporation of trivalent actinide (An(III)) [2], and investigating applications in the fields of catalysis, ion exchange, sensors, and photochemistry [3-5].
Ultrathin sections were stained with
uranyl acetate and lead citrate and observed in a Philips EM 400.
The deeply yellow-colored
uranyl nitrate solution consisted of 21 mg U[O.sup.2.sup.+2] per gram of solution in (1.06 [+ or -] 0.01) mol [L.sup.-1] HN[O.sub.3] with a density of (1.057 [+ or -] 0.001) g m[L.sup.-1] at 21.6 [degrees]C.
As a proof-of-concept, aptamer against
uranyl ion was used for the decontamination of uranium.
12 [micro]l of the dilutions were allowed to adsorb on carbon coated copper grid for 1 min followed by negative staining with aqueous
uranyl acetate (0.5%).
