SYBR Safe
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| Names | |
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| IUPAC name
(Z)-4-((3-Methylbenzo[d]thiazol-2(3H)-ylidene)methyl)-1-propylquinolin-1-ium 4-methylbenzenesulfonate
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| Identifiers | |
1030826-36-8  |
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| Properties | |
| C28H28N2O3S2 | |
| Molar mass | 504.66 g/mol |
| Solubility | Soluble in Dimethylsulfoxide |
| Vapor pressure | {{{value}}} |
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Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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| Infobox references | |
SYBR Safe is a cyanine dye[1] used as a nucleic acid stain in molecular biology. SYBR Safe is one of a number of SYBR dyes made by the Life Technologies Corporation. SYBR Safe binds to DNA. The resulting DNA-dye-complex absorbs blue light (λmax = 509 nm) and emits green light (λmax = 524 nm).
Uses
SYBR Safe is most commonly used as a DNA stain for identifying DNA in agarose gels.
Safety
SYBR Safe is a safer alternative to the known mutagen ethidium bromide. Interestingly, SYBR Safe is has a very similar structure as thiazole orange, which has a methyl group attached to the charged nitrogen where as SYBR Safe has an N-propyl group. Thiazole Orange has been shown to be three to four times less mutagenic as ethidium bromide whereas SYBR Safe is four to five less mutagenic.[2] Additionally, according to the Life Technologies website, SYBR Safe is not lethal in rats at doses as high as 5 g/kg, and rats don't show symptoms of acute toxicity. Thiazole orange does show toxicity at this dose.
Similar cyanine dyes
- TO (Thiazole Orange)
- SYBR Green I
- SYBR Green II
- SYBR Gold
- YO (Oxazole Yellow)
- PG (PicoGreen)
