TRIM5alpha

Tripartite motif containing 5
250px Rendering based on PDB 2ECV.
Available structures PDB Ortholog search: PDBe, RCSB List of PDB id codes 2ECV, 2YRG
Identifiers
Symbols	TRIM5 ; RNF88; TRIM5alpha
External IDs	OMIM: 608487 MGI: 2441735 HomoloGene: 75345 GeneCards: TRIM5 Gene
EC number	6.3.2.-
Gene ontology Molecular function • ubiquitin-protein transferase activity • protein binding • zinc ion binding • signaling pattern recognition receptor activity • ligase activity • protein kinase binding • protein binding, bridging • identical protein binding • protein homodimerization activity Cellular component • cytoplasmic mRNA processing body • cytoplasm • cytosol • omegasome Biological process • activation of innate immune response • pattern recognition receptor signaling pathway • autophagy • viral process • cytokine-mediated signaling pathway • regulation of lipopolysaccharide-mediated signaling pathway • regulation of protein localization • positive regulation of I-kappaB kinase/NF-kappaB signaling • positive regulation of MAPK cascade • innate immune response • negative regulation of viral entry into host cell • positive regulation of sequence-specific DNA binding transcription factor activity • positive regulation of NF-kappaB transcription factor activity • defense response to virus • interferon-gamma-mediated signaling pathway • protein trimerization • protein K63-linked ubiquitination • negative regulation of viral release from host cell Sources: Amigo / QuickGO
RNA expression pattern
File:PBB GE TRIM5 210705 s at tn.png
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	85363	319236
Ensembl	ENSG00000132256	ENSMUSG00000057143
UniProt	Q9C035	n/a
RefSeq (mRNA)	NM_033034	NM_001146007
RefSeq (protein)	NP_149023	NP_001139479
Location (UCSC)	Chr 11: 5.66 – 5.94 Mb	Chr 7: 104.34 – 104.35 Mb
PubMed search	[1]	[2]

Tripartite motif-containing protein 5 also known as RING finger protein 88 is a protein that in humans is encoded by the TRIM5 gene. The alpha isoform of this protein, TRIM5α, is a retrovirus restriction factor, which mediates species-specific, early block to retrovirus infection.

TRIM5α is composed of 493 amino acids that is found in the cells of most primates. TRIM5α is an intrinsic immune factor important in the innate immune defense against retroviruses, along with the APOBEC family of proteins, tetherin and TRIM22.

Structure

TRIM5α belongs to the TRIM protein family (TRIM stands for TRIpartite Motif); this family was first identified by Reddy in 1992 as the proteins that contain a RING finger zinc binding domain, a B-box zinc binding domain, followed by a coiled-coil region. TRIM5α bears the C-terminal PRY-SPRY or B30.2 domain in addition to the other domains.

Function

When a retrovirus enters a host cell's cytoplasm, it undergoes processes such as capsid uncoating and reverse transcription. TRIM5 present in the cytoplasm recognizes motifs within the capsid proteins and interferes with the uncoating process, therefore preventing successful reverse transcription and transport to the nucleus of the viral genome. The exact mechanism of action has not been shown conclusively, but capsid protein from restricted viruses is removed by proteasome-dependent degradation.

The involvement of other cellular proteins in the inhibition mediated by TRIM5α is suspected but as yet not demonstrated. However, Cyclophilin A is important for the inhibition of HIV-1 by TRIM5α in Old World monkey species.

The "specificity" of restriction, that is, whether a given retrovirus can be targeted by TRIM5α, is entirely determined by the amino acid sequence of the C-terminal domain of the protein, called the B30.2/PRY-SPRY domain. Amino acid 332, which occurs within this domain, seems to play a critical role in determining the specificity of retrovirus restriction.

TRIM5α may have played a critical role in the human immune defense system about 4 million years ago, when the retrovirus PtERV1 was infecting the ancestors of modern chimpanzees. While no trace of PtERV1 has yet been found in the human genome, about 130 traces of PtERV1 DNA have been found in the genome of modern chimpanzees. After recreating part of the PtERV1 retrovirus, it was reported that TRIM5α prevents the virus from entering human cells in vitro. While this cellular defense mechanism may have been very useful 4 million years ago when facing a PtERV1 epidemic, it has the side effect of leaving cells more susceptible to attack by the HIV-1 retrovirus. Recently, doubt has been cast over these conclusions. By using a PtERV1 capsid, which produces higher titer virus-like particles, Perez-Caballero et al. reported that PtERV1 is not restricted by either human or chimpanzee TRIM5α.

Clinical significance

Old World monkeys cannot be infected with HIV-1, the virus that causes AIDS in humans; they can be infected, however, with SIV, a related virus. TRIM5α was isolated as a rhesus macaque protein responsible for blocking infection by HIV-1.

The human version of TRIM5α does not target HIV-1, but can inhibit strains of the murine leukemia virus (MLV) as well as equine infectious anemia virus (EIAV).

Prior to the discovery of TRIM5α as an antiviral protein, the inhibition phenotype had been described and coined Ref1 (in human cells) and Lv1 (in monkey cells). This terminology is now largely abandoned.

A related protein, named TRIMCyp (or TRIM5-CypA), was isolated in the owl monkey, a species of New World monkey, and shown to potently inhibit infection by HIV-1. A similar protein has arisen independently in Old World monkeys and has been identified in several species of macaque.

See also

• Peptidylprolyl isomerase A