皇后盃第43回全国都道府県対抗女子駅伝は2025年1月12日、京都市右京区のたけびしスタジアム京都を発着点に京都市左京区の国立京都会館前を折り返す9区間、42・195キロで行われ、京都が中盤から独走態勢を築き、2時間15分26秒で3年ぶり19度目の優勝を飾った。2位は2時間17分52秒で大阪、1秒遅れの3位に福岡が入った。

The 43rd Empress's Cup National Inter-Prefectural Women's Ekiden will be held on January 12, 2025, covering nine sections and 42.195 km, starting and ending at Takebishi Stadium Kyoto in Ukyo Ward, Kyoto City, and wrapping up in front of the National Kyoto Kaikan Hall in Sakyo Ward, Kyoto City. Kyoto took the lead from the middle of the race, winning in 2 hours 15 minutes 26 seconds to take its 19th victory in three years. Osaka came in second with a time of 2 hours 17 minutes 52 seconds, and Fukuoka came in third, one second behind.