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    10 Chapter 10 Answers

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    Student A counted bacteria from soil using two media - R2A and nutrient agar. Higher counts were found on R2A since it allows growth of oligotrophic bacteria found in soil, unlike the nutrient-rich nutrient agar. Student B counted bacteria from soil poured onto plates and calculated the dilution plate containing 80 colonies was 10^-7. Student C's direct count of bacteria in the same soil as Student A would exceed A's culturable count as direct counts include non-culturable bacteria. C's count after letting the soil extract sit overnight would also likely exceed A's due to potential bacterial growth in the extractant.

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    10 Chapter 10 Answers

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    157 views2 pages
    Student A counted bacteria from soil using two media - R2A and nutrient agar. Higher counts were found on R2A since it allows growth of oligotrophic bacteria found in soil, unlike the nutrient-rich nutrient agar. Student B counted bacteria from soil poured onto plates and calculated the dilution plate containing 80 colonies was 10^-7. Student C's direct count of bacteria in the same soil as Student A would exceed A's culturable count as direct counts include non-culturable bacteria. C's count after letting the soil extract sit overnight would also likely exceed A's due to potential bacterial growth in the extractant.

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    jawaban 10 soal

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    10~Chapter_10_Answers

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    Student A counted bacteria from soil using two media - R2A and nutrient agar. Higher counts were found on R2A since it allows growth of oligotrophic bacteria found in soil, unlike the nutrient-rich nutrient agar. Student B counted bacteria from soil poured onto plates and calculated the dilution plate containing 80 colonies was 10^-7. Student C's direct count of bacteria in the same soil as Student A would exceed A's culturable count as direct counts include non-culturable bacteria. C's count after letting the soil extract sit overnight would also likely exceed A's due to potential bacterial growth in the extractant.

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    © All Rights Reserved
    Download as RTF, PDF, TXT or read online from Scribd
    Download as rtf, pdf, or txt
    157 views2 pages

    10 Chapter 10 Answers

    Uploaded by

    oppinokio
    Student A counted bacteria from soil using two media - R2A and nutrient agar. Higher counts were found on R2A since it allows growth of oligotrophic bacteria found in soil, unlike the nutrient-rich nutrient agar. Student B counted bacteria from soil poured onto plates and calculated the dilution plate containing 80 colonies was 10^-7. Student C's direct count of bacteria in the same soil as Student A would exceed A's culturable count as direct counts include non-culturable bacteria. C's count after letting the soil extract sit overnight would also likely exceed A's due to potential bacterial growth in the extractant.

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    Chapter10

    CulturalMethods
    IanL.PepperandCharlesP.Gerba
    1.

    StudentAperformsasoilextractionwithpeptoneastheextractingsolution.Adilutionandplating
    techniqueisperformedusingthespreadplatemethod.Thefollowingresultsareobtained:
    a.150coloniesarecountedonanR2Aplateatthe105dilution
    b.30coloniesarecountedonanutrientagarplateat104dilution
    CalculatethenumberofviablebacteriapergramofsoilforR2Aandnutrientagar.Why
    arethecountsfornutrientagarlowerthanfortheR2Amedia?
    NumberofviablebacteriapergramofsoilfromR2A:.
    Assuming0.1mlofsoilextractisspreadplated.
    N=1501/106=1.5108CFU/gsoil
    Numberofviablebacteriapergramofsoilfromnutrientagar.
    N=301/105=3106CFU/gsoil.
    Countsfromnutrientagararelowersinceitisanutrientrichmediumwhichdoesnotallowforthe
    growthofoligotrophicbacteriafoundinsoil.R2Aisanutrientpoormediumthatdoesallowfor
    oligotrophicgrowth.

    2.

    StudentBperformsasoilextractionwithpeptoneastheextractingsolution.Adilutionandplating
    techniqueisperformedusingthepourplatemethod.Thenumberofbacteriapergramof
    soiliscalculatedtobe8108.Whatdilutionplatecontained80colonies?
    Sincethepourplatemethodisused,assume1mlofsoilextractisplated.

    Number

    of CFU

    / g soil

    for80colonies

    80

    Dilution

    8 10

    dilution

    80
    8 10

    10

    # colonies
    dilution

    8 10

    3.

    StudentCuseddirectmicroscopicmethodstocountbacteriainthesamesoilasstudentA
    (seeproblem1).HowwouldstudentCsnumberscomparetostudentAsnumbers
    andwhy?
    ThenumberforstudentC(directcounts)wouldexceedthenumberforstudentA
    (culturablecount)sincethedirectcountalsomeasuresviablebutnonculturable,viable
    butdifficulttoculture,andalsononviablebacteria.

    4.

    StudentCalsoextractedthesamesoilinpeptonebutlettheextractionsitovernightat
    roomtemperaturebeforedilutingandplating.Howwouldthesenumberscompare
    tostudentAsnumbersandwhy?
    ThenumberforstudentCwouldmostlikelyexceedstudentAsnumbersduetopotential
    growthofbacteriawithinthepeptoneextractant.

    5.

    Identifythemajorerrorsassociatedwithdilutionandplatingtechniques.
    Therearemanymajorerrors,including:
    a.Only110%ofsoilbacteriaareculturable.
    b.Noanaerobesarecultured.
    c.Theassumptionof1colonyarisingfrom1cellisusuallyinvalidduetocell
    clumping,resultinginanunderestimationofCFUs.
    d.Sedimentationofsoilduringpreparationofdilutionscanleadtoan
    underestimationofCFUs.

    6.

    Whatisthedifferencebetweenaprimarycelllineandacontinuouscellline?Whichcell
    linedoyouthinkisbetterforisolatingvirusesfromtheenvironment?Why?
    Inprimarycellculturethecellscomedirectlyfromtheanimalandonlycanbepasseda
    limitednumberoftimes.Incontrast,continuouscellslinescanbepassed

    indefinably.

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