Biology f212 Mixed Question Paper

1.
A balanced diet is essential for good health.

Complete the following passage by using the most appropriate terms from the list to fill
the gaps.
Each term should not be used more than once.
haemoglobin
calcium
iron
collagen
anorexic
obese
sodium
A balanced diet is one which provides an adequate intake of energy and nutrients for
the maintenance of our body. If energy intake exceeds energy usage over a period of
time, an individual can become . .
The deficiency disease anaemia can be caused by a lack of the mineral
. in the diet. As a result of this deficiency, the body is unable to
produce sufficient amounts of the protein . in red blood cells.
[Total 3 marks]
2.
The Body Mass Index (BMI) is one way of determining whether a person is
underweight or overweight.
BMI can be calculated using the formula:
BMI =
mass in kg
(height in m) 2
Calculate the BMI of a female of mass 69 kg and a height of 1.67 m.

Show your working. Give your answer to one decimal place.
Answer = ..........................................
[Total 2 marks]
3.
A way of determining whether a person is underweight or overweight is to use a graph

showing the relationship between height and body mass.
The figure below is an example of this type of graph.
underweight
acceptable
1.90
overweight
morbidly
obese
1.80
height (m)
obese
1.70
1.60
1.50
40
50
60
70
80
90
100 110 120 130 140 150

mass (kg)
(i)
Using the figure above, state the category into which a female who has a body
mass of 69 kg and a height of 1.67 m is placed.
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[1]
(ii)
There are many factors that determine the category into which a person is
placed. The figure above does not take into account all of these factors.
Suggest why the female in (i) might be placed in the wrong category.
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[2]
[Total 3 marks]
4.
Name two diseases associated with obesity.

1 ...............................................................................................................................
2 ...............................................................................................................................
[Total 2 marks]
5.
The figure below represents a water molecule.

H
Water molecules are polar. As a result, they attract each other.

Draw a second water molecule on the figure above.
Your drawing should show:
the bond(s) between the two molecules

the name of the bond
the charges on each atom.
[Total 3 marks]
6.
Ponds provide a very stable environment for aquatic organisms.

Three properties of water that contribute to this stability are as follows:
the density of water decreases as the temperature falls below 4 C so ice floats
on the top of the pond
it acts as a solvent for ions such as nitrates (NO3)
a large quantity of energy is required to raise the temperature of water by 1 C.
Explain how these three properties help organisms survive in the pond.
In your answer you should make clear the links between the behaviour of the water
molecules and the survival of the organisms.
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[Total 8 marks]
7.
Water is important in many biological reactions.

Complete the table below by writing an appropriate term next to each description.
description
term
the type of reaction that occurs

when water is added to break a
bond in a molecule
the phosphate group of a
phospholipid that readily attracts
water molecules
[Total 2 marks]
8.
The enzyme DHPS is involved in the production of folic acid in bacteria.
The substrate for DHPS is a molecule known as PABA.
The enzyme DHPS is inhibited by the drug sulfonamide.
The figure below shows the structure of PABA and that of sulfonamide.
H
N
O
HO
N
H
N
H
PABA
sulfonamide
(i)
Diagrams X, Y and Z represent these enzyme molecules and their active sites.
State the letter, X, Y or Z, that most accurately represents the enzyme DHPS.
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[1]
(ii)
Using the information in the figure above, explain why sulfonamide acts as a
competitive inhibitor of DHPS.
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[3]
[Total 4 marks]
9.
The figure below shows the effect of increasing the concentration of the substrate
(PABA) on the rate of reaction.
Curve A shows the rate of reaction without the presence of the competitive
inhibitor sulfonamide.
Curve B shows the rate of reaction in the presence of the competitive inhibitor
sulfonamide.
Key:
A = without inhibitor
B = with inhibitor
rate of
reaction
(arbitrary
units)
concentration of substrate
PABA (arbitrary units)
Explain the effect of increasing the concentration of substrate on the rate of reaction;
(i)
without inhibitor,
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[3]
(ii)
with inhibitor.
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[2]
[Total 5 marks]
10.
Antibiotic resistance in bacteria is becoming an increasing problem.

Describe how a sulfonamide-resistant population of bacteria could develop.
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[Total 4 marks]
11.
Hospitals can check to see if a strain of bacteria causing an infection is resistant to a

range of antibiotics by using a multodisc. A multodisc contains different antibiotics.
The bacteria are isolated from a patient.

The bacteria are spread on nutrient agar in a Petri dish.
The multodisc is placed on the agar.
The figure below shows a Petri dish with the bacteria, in which is placed a multodisc
containing six different antibiotics.
clear
area
Key:
1
6
1 tetracycline
2
2 amoxicillin
3 neomycin
Petri dish
with nutrient
agar
multodisc
4 streptomycin
5 penicillin
6 sulfonamide
4
bacteria present
(i)
Explain why there are clear areas of agar in the Petri dish.
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[1]
(ii)
Using the figure above, name the antibiotic that is most effective against the
bacteria causing the infection.
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[1]
(iii)
Suggest three reasons why a hospital might use a multodisc to select the most
suitable antibiotic for treating a patient.
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[Total 5 marks]
12.
Drugs, such as antibiotics, are often first discovered in the natural environment.
Explain why it may become increasingly difficult to discover new drugs in the future.
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[Total 2 marks]
13.
(a)
Amino acids are the basic building blocks for proteins. The figure below shows
the amino acid cysteine.
M
CH2
SH
OH
N
L
O
H
K
(i)
Complete the table by selecting the letter, J, K, L or M, that represents the

following groups in cysteine.
group
letter
carboxyl
R group
amine group
[3]
(ii)
The primary structure of a protein consists of a chain of amino acids.

Describe how a second amino acid would bond to cysteine in forming the
primary structure of a protein.
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[3]
(b)
Each amino acid has a different R group.

Describe how these R groups can interact to determine the tertiary structure of a
protein.
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[4]
[Total 10 marks]
14.
The figure below shows the structure of two polymers, glycogen and collagen, that are
found in mammals.
glycogen
collagen
(i)
Complete the table below to give three differences between the structure of
glycogen and collagen.
glycogen
collagen
[3]
(ii)
Collagen is found in the ligaments which hold bones together at joints.

State two properties of collagen that make it suitable for this purpose.
1 ......................................................................................................................
2 ......................................................................................................................
[2]
[Total 5 marks]
15.
Coronary heart disease (CHD) can be described as a multifactorial disease. This

means that a number of different risk factors contribute to the development of the
disease.
The figure below shows the percentage of cases of CHD in a population to which each
risk factor contributed.
CHD due to lack of
exercise (37%)
all CHD
CHD due to high

blood cholesterol
(46%)
CHD due to high

blood pressure
(13%)
(i)
CHD due to
smoking (19%)
When you add up the different risk factor percentages for the population you find
that it is greater than 100%.
Suggest why.
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[1]
(ii)
State two further risk factors that are not shown in the figure above.
1 ......................................................................................................................
2 ......................................................................................................................
[2]
(iii)
Smoking is a contributing factor in 19% of all cases of CHD.

The table below lists a number of effects of cigarette smoke.
Use a tick ( ) to indicate which component of cigarette smoke causes each
effect.
The first row has been done for you.
effect
nicotine
carbon
monoxide
increases heart rate

constricts arterioles
damages the lining of arteries
reduces the ability of
haemoglobin to carry oxygen
makes platelets sticky
[4]
[Total 7 marks]
16.
Cholesterol is transported in the form of lipoproteins. High levels of low density

lipoproteins (LDLs) in the blood are a risk factor in heart disease.
Outline the role of LDLs in the formation of an atheroma.
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[Total 2 marks]
17.
An atheroma can grow to a point where it restricts blood flow in a coronary artery,
causing coronary heart disease (CHD).
The figure below shows a method of reducing the symptoms of CHD.
A stent is a tubular device, containing a balloon, which can be inserted into the
damaged artery. The stent can be opened up by inflating the balloon.
Explain how the inserted stent would reduce the symptoms of CHD.
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[Total 4 marks]
18.
DNA and RNA are nucleic acids.

(i)
State the components of a DNA nucleotide.

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[3]
(ii)
Describe how the structure of RNA differs from that of DNA.

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[2]
[Total 5 marks]
19.
Before a cell divides, the DNA needs to be accurately replicated.

Describe how a DNA molecule is replicated.
In your answer you should make clear how the steps in the process are sequenced.
[Total 7 marks]
20.
(i)
State what a gene codes for.

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[1]
(ii)
Suggest how changing the sequence of DNA nucleotides could affect the final
product the DNA codes for.
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[2]
[Total 3 marks]
21.
Fig. 1 below shows a photograph of a part of a heathland habitat. A study was carried
out on the biodiversity of this habitat.
Fig. 1
(a)
Define the terms:

habitat .............................................................................................................
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biodiversity ......................................................................................................
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[3]
(b)
In this study, a student placed his quadrat on areas he considered to have the
most biodiversity.
Explain what is wrong with this technique.
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[2]
(c)
The student looked at the abundance of three plants at different distances from
the bottom of the slope.
The results table drawn by the student is shown below.
distance from
bottom of slope
(i)
percentage cover of each plant species

cotton grass
ling
bracken
0m
76
10 m
68
20 m
30 m
35
40 m
50
50 m
60
60 m
40
17
70 m
10
42
80 m
68
90 m
71
100 m
74
The format of the students table is incorrect.

Suggest one way in which the student could correct the table.
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[1]
Fig. 2 is a graph showing the distribution of cotton grass and bracken at different
distances from the bottom of the slope.
100
cotton grass
%
cover
bracken
50
0
0
bottom
of slope
100
distance (m)
top of
slope
Fig. 2
(ii)
Using the information given in the table above, sketch on Fig. 2 a curve to
show the distribution of ling.
[3]
(iii)
Describe the distribution of bracken.

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[2]
(d)
(i)
The student was asked to calculate the biodiversity using Simpsons Index
of Diversity.
Suggest what additional data he would need to collect in order to calculate
Simpsons Index of Diversity in this habitat.
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[2]
(ii)
The student calculated Simpsons Index as 0.2. This is a low value.

State the significance of this low value for this habitat.
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[1]
[Total 14 marks]
22.
Complete the following passage by using the most appropriate terms from the list to fill
the gaps.
Each term should not be used more than once.
-pleated sheet
anti-parallel
covalent
double helix
hydrogen
parallel
polypeptide
ribose
sugar-phosphate
DNA is found in the nucleus. The molecule is twisted into a ..................................

.................................. in which each of the strands are .......................................... . It
has two ........................................................ backbones attached to one another by
complementary bases. These bases pair in the centre of the molecule by means of
................................. bonds.
[Total 4 marks]
23.
The table below shows the relative proportions of different DNA bases in four different
organisms.
relative proportions of bases in DNA

as a percentage
organism
human
30.9
19.8
19.9
29.4
grasshopper
29.3
20.7
20.5
29.3
wheat
27.3
22.8
22.7
27.1
E. coli
24.7
25.7
26.0
23.6
(i)
Describe the patterns shown by the data given in the table above.
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[3]
(ii)
Suggest how the data given in the table above might have been helpful to
scientists in working out the structure of DNA.
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[2]
[Total 5 marks]
24.
DNA in the nucleus acts as a template for the production of RNA.

Complete the table below to show three ways in which the structure of DNA differs
from that of RNA.
feature
DNA
RNA
number of strands
bases present
sugar present
[Total 3 marks]
25.
DNA codes for the structure of polypeptides.

State the role of messenger RNA (mRNA).
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[Total 2 marks]
26.
Malaria kills over one million people every year, the vast majority being under the age
of ten.
Adults who have survived malaria in childhood and then continue to live in an area
where malaria is found, develop a limited form of immunity.
(a)
(i)
Name the parasite that causes malaria.

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[1]
(ii)
Name the vector for the malarial parasite.

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[1]
(iii)
Name a human cell in which the malarial parasite reproduces.

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[1]
(b)
Scientists are developing a vaccine using an attenuated (inactive) form of the

malarial parasite.
The aim is to trigger an immune response without the development of the
disease.
Describe the actions of the B lymphocytes in the immune response.
In your answer you should make clear how the steps in this part of the immune
response are sequenced.
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[8]
(c)
Suggest why adults who have survived malaria may lose their immunity when
they leave a malarial area.
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[2]
(d)
State three biological reasons why it has not been possible to produce an
effective vaccine for malaria.
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[Total 16 marks]
27.
In the search for new biofuels, research has been done into the digestion of wood
waste by fungi.
The cellulase enzymes produced by the fungi break cellulose into sugars. These
sugars can then be converted into ethanol, a biofuel.
The figure below shows the stages in this digestion process.
O
OH
OH
A
OH
cellulose
OH
O
O
O
O
enzyme 1
B
O
disaccharide
O
enzyme 2
CH2OH
H
OH
O
H
OH H
H
(a)
(i)
OH
H
OH
Name bonds A and B shown in the figure.

A .............................................................................................................
B .............................................................................................................
[2]
(ii)
State how bond B is broken in the digestion of the disaccharide.

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[1]
(iii)
Name the sugar that is the final product of this digestion process.
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[1]
(b)
Explain why different enzymes are involved in each stage of the digestion
process.
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[3]
(c)
In the search for new biofuels, research has been done into the digestion of wood
waste by fungi.
The cellulase enzymes produced by the fungi break cellulose into sugars. These
sugars can then be converted into ethanol, a biofuel.
The figure below shows the stages in this digestion process.
O
O
cellulose
OH
OH
A
OH
OH
O
O
O
O
enzyme 1
B
O
disaccharide
O
enzyme 2
CH2OH
H
OH
O
H
OH H
H
OH
OH
H
The figure below shows the effect of changing pH on the rate of activity of
enzyme 2.
rate of enzyme activity (arbitrary units)
50
40
30
20
10
0
1
pH
(i)
Explain why the activity of enzyme 2 falls to zero at pH 7.

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[3]
(ii)
State two factors that should have been controlled when investigating the
effect of pH on the activity of enzyme 2.
1 .............................................................................................................
2 .............................................................................................................
[2]
[Total 12 marks]
28.
The activity of an enzyme can be measured by testing for the concentration of its
product at regular intervals.
Describe how the concentration of a reducing sugar can be measured using a
colorimeter.
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[Total 6 marks]
29.
In India, the population of the white-backed vulture, Gyps bengalensis, has fallen by
97% to an estimated 4 000 vultures. This vulture is now considered to be critically
endangered. Reasons for the decline in numbers include:
vultures feed on carcasses including those from farm animals.
hese farm animals may have been treated with a pain killer. This particular pain
killer causes kidney failure in the vultures.
the use of this pain killer is being phased out. However, many farmers continue to
use up their stocks of the drug.
this pain killer is not easily biodegradable and will remain in the environment for
many years.
(i)
Suggest what is meant by critically endangered.

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[1]
(ii)
Calculate the original population of the white-backed vulture.

Show your working.
Answer = ........................................................
[2]
[Total 3 marks]
30.
many years.
In an effort to save the white-backed vulture, a captive breeding programme has been
set up.
Three centres in India have been built, each housing up to 40 individuals. These
vultures have been collected from different areas of the Indian subcontinent.
(i)
Explain why the decision was made to conserve the species in captivity (ex situ)
rather than in the wild (in situ).
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[4]
(ii)
Explain why the white-backed vultures in the captive breeding programme were,
collected from several different areas
housed in three separate centres.
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[Total 7 marks]
31.
many years.
set up.
Outline three reasons why the conservation of the white-backed vulture is important.
1 ...............................................................................................................................
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[Total 3 marks]
32.
many years.
set up.
Suggest three measures that could be taken in the long term to preserve the
numbers of white-backed vultures, once the captive bred individuals have been
released into the wild.
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[Total 3 marks]
33.
The figure below shows a section of a leaf from a pear tree that is infected by the
mildew fungus.
spore
nuclei
wall
upper
epidermis
fungalh ypha
(secretesenzymes
todigestcell)
mesophyll
cells
chloroplast
(i)
State one feature, shown in the figure above, that excludes both the pear tree
and mildew from the kingdom Prokaryotae.
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[1]
(ii)
State two reasons why mildew should be placed in the kingdom Fungi.
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[2]
(iii)
State two reasons why the pear tree should be placed in the kingdom Plantae.
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[2]
(iv)
Name two kingdoms other than Prokaryotae, Fungi and Plantae.

1 ......................................................................................................................
2 ......................................................................................................................
[2]
[Total 7 marks]
34.
The mildew fungus also infects wheat plants, causing disease.
Most wheat plants in the UK show little resistance to this disease.
Some Iranian wheat plants are resistant.
The yield from these resistant wheat plants is very low.
(i)
An investigation into the resistance of the Iranian wheat plants to mildew

produced the results shown in the figure below.
numbers of
plants
low
resistance
high
resistance
State the type of variation that is shown in the figure above and describe its
characteristics.
type of variation ..............................................................................................
characteristic of this type of variation
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[3]
(ii)
Outline how a breeding programme could be carried out to produce wheat plants
which have both high yield and resistance to mildew.
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[3]
[Total 6 marks]
35.
Over a period of time, mildew can overcome the resistance bred into the wheat.
Use the theory of natural selection to explain how the mildew fungus adapts to
overcome this resistance.
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[Total 4 marks]
36.
The figure below shows a diagram of alveoli in a healthy lung and alveoli in a lung from
a person with advanced emphysema.
interior of alveoli
alveoli in a healthy lung
alveoli from a person with advanced emphysema
(i)
Describe how smoking could cause changes in alveoli, such as those shown in
the figure above.
In your answer you should make the links between the changes and their causes
clear.
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[6]
(ii)
Emphysema is a form of chronic obstructive pulmonary disease (COPD).

Describe two signs or symptoms of emphysema.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(iii)
Emphysema is described as a chronic disease.

Suggest the meaning of the term chronic.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 10 marks]
37.
An investigation was conducted into the effect of smoking on lung function. One
measure of lung function is peak flow rate.
The peak flow rate is the maximum volume of air expelled from the lungs in one minute
(dm3 min1).
Two male volunteers, one a smoker and one a non-smoker, had their peak flow
measured once a year for seven years.
Key:
non smoker
smoker
375
350
325
peak flow rate
(dm3 min1)
300
275
250
13
14
15
16
age (years)
17
18
19
(i)
Describe the data shown in the figure above.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
(ii)
Explain the results obtained for the smoker.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(iii)
Suggest three ways of improving the reliability of this investigation.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 9 marks]
38.
Some species of Acacia tree produce gum arabic. Gum arabic is classed as a
heteropolysaccharide. This means that it is made up of a number of different sugars.
Hydrolysis of gum arabic releases four different monosaccharides.
Describe what happens during the hydrolysis of a polysaccharide molecule.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
39.
Complete the table below, comparing gum arabic with some other polysaccharides.
gum arabic
amylase
cellulose
branched structure
yes
no
heteropolysaccharide
yes
no
plants
plants
found in
animals/plants
function in organism
healing cuts
glycogen
energy store
[Total 4 marks]
40.
Acacia senegal is a species of tree which is common in the drier parts of Africa. Cattle
are allowed to graze on both its leaves and the fallen seed pods. The seed pods have
relatively high protein content.
(i)
Describe how you would test an extract of the seed pods for protein.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
Describe how you could compare the reducing sugar content of the leaves with
that of the seed pods.
In your answer you should make clear how the steps in the process are
sequenced.
[8]
(iii)
The seeds of Acacia species are sometimes eaten by people.

Suggest why it might be better for people living in areas where the tree grows to
let their cattle feed on the trees and fallen seed pods and then obtain their
nutrition from the cattle.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 13 marks]
41.
DNA and RNA are nucleic acids.

(i)
Describe the structure of a DNA nucleotide.

In your answer you should spell the names of the molecules correctly.
You may use the space below to draw a diagram if it will help your description.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
Describe how the two nucleotide chains in DNA are bonded together.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 7 marks]
42.
State three ways in which the structure of DNA differs from that of RNA.
1 ...............................................................................................................................
..................................................................................................................................
2 ...............................................................................................................................
..................................................................................................................................
3 ...............................................................................................................................
..................................................................................................................................
[Total 3 marks]
43.
An antibody is an example of a protein molecule, which has a specific 3-dimensional

shape.
The diagram below shows the structure of an antibody molecule.
constant region
variable region
heavy polypeptide
chains
light polypeptide
chain
(i)
Outline how the structure of an antibody molecule is related to its function.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
Suggest why the base sequence in the genes for human antibodies is more
similar to that found in a chimp than to that found in a mouse.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 5 marks]
44.
The fungus, yeast, contains the enzyme catalase.

Catalase speeds up the decomposition of hydrogen peroxide, a toxic metabolic
product, to oxygen and water.
2 H2O2 2 H2O + O2
A student decided to investigate the activity of catalase using the apparatus shown in
Fig. 1.
The total volume of gas collected was recorded every 20 seconds.
The results are shown in Fig. 2.
10cm3 of 5%
hydrogen peroxide
mixed with 5cm3 of
yeast suspension
graduated
cylinder
water
Fig. 1
10
9
8
7
volume
of
6
oxygen/cm3
5
4
3
2
1
0
0
20
40
60
80
100
120
140
time/s
Fig. 2
The rate of decomposition can be calculated using the formula:

rate of decomposition =
(a)
volume of oxygen collected

time taken for collection
Calculate the rate of decomposition over the first 30 seconds.

Show your working and give your answer in cm3 min1.
.......................................cm3 min1
[2]
(b)
The initial rate of decomposition is the rate measured within the first few seconds.
Using the dashed line in Fig. 2, the initial rate of decomposition is calculated to be
19 cm3 min1.
Explain why the initial rate of reaction is greater than the rate you calculated
in (a).
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 5 marks]
45.
Fungi such as Fusarium venenatum are grown in huge batch cultures to manufacture
protein for food products.
Explain why these cultures are often maintained at the optimum temperature for protein
production and not at a temperature above the optimum.
In your answer you should make clear how the structure and activity of enzymes
relates to the effects described.
[Total 8 marks]
46.
A group of students carried out some fieldwork to investigate the diversity of insects in
three habitats:
a field of barley
a field of wheat
the vegetation under a hedge.
Their results are shown in the table below. The table also shows how they used their
data to calculate Simpsons Index of Diversity (D) for each habitat.
D = 1 ((n/N)2)
where N = the total number of insects found, and n is the number of individuals of a
particular species.
number of individuals of each
species
in each habitat
species
barley
field
wheat
field
under
hedge
32
78
126
12
25
10
86
56
species richness
10
196
226
52
0.61
0.86
total number of
insects (N)
Simpsons Index
of Diversity (D)
(a)
State what is meant by the term species richness.

.........................................................................................................................
.........................................................................................................................
[1]
(b)
(i)
Calculate the value for Simpsons Index of Diversity (D) for the barley field.
Show your working and write your answer in the shaded box in the table.
[2]
(ii)
Using the data in the table, suggest why the value of Simpsons Index
of Diversity (D) for the vegetation under the hedge is so much higher
than that for the wheat field.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[3]
(c)
Describe how the students may have determined the numbers of individuals of
each species in each habitat.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[5]
[Total 11 marks]
47.
Studies of biodiversity are an integral part of an environmental impact assessment

(EIA).
(i)
Discuss the role of an EIA as part of a local planning decision.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
Suggest why some conservationists might object to these studies.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 5 marks]
48.
The leopard, Panthera pardus, is a large member of the cat family.

Complete the following table to show the full classification of the leopard.
Kingdom
Class
..
Family
Genus
Chordata
Mammalia
Carnivora
Felidae
pardus
[Total 5 marks]
49.
The leopard belongs to a kingdom in which all members are eukaryotic. Plants are also
eukaryotic.
Name two other kingdoms that contain eukaryotic organisms.
1 ...............................................................................................................................
2 ...............................................................................................................................
[Total 2 marks]
50.
Historically, all organisms were classified into just two kingdoms. In 1988 a fivekingdom system of classification was accepted. In 1990 a three domain system was
proposed.
Discuss, with reference to the Prokaryotes, the reasons why classification systems are
not universally accepted and why they change over time.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 4 marks]
51.
(a)
Staphylococcus aureus is a species of bacterium that is found on the skin.

(i)
Describe how variation may arise within a species of bacterium such as

S. aureus.
................................................................................................................
................................................................................................................
................................................................................................................
[1]
(ii)
Suggest why such variation alters the characteristics of the individual

organism.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(b)
Discuss the difficulties that variations arising in S. aureus may cause to the
medical profession.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 7 marks]
52.
The Human Immunodeficiency Virus (HIV) is spread by exchange of body fluids

between an infected person and an uninfected person. This often occurs as a result of
unprotected sexual intercourse.
The diagram below shows the percentage of people infected with HIV in different parts
of the world at the end of 2002.
Western Europe
0..5 1%
North America
0.5 1%
Caribbean
15%
Latin America
1 5%
(i)
Eastern Europe
& Central Asia
1 5%
North Africa & Middle East

0.5 1%
East Asia & Pacific

0.5 1%
South
& South-East Asia
0.5 - 1%
Sub-Saharan Africa
15 39%
%
Australia
& New Zealand
0.1%
The percentage of people infected with HIV is much higher in Sub-Saharan Africa
than in much of Europe.
Suggest three reasons why the percentages are so much higher in Sub-Saharan
Africa.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
Explain why it is useful to collect information, such as that shown in the figure
above.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 7 marks]
53.
At present there is no cure for HIV / AIDS. Researchers have found that some people
in Africa are not infected despite continual exposure to the disease. HIV uses a specific
cell surface receptor known as the CD4 receptor to enter a human cell.
Suggest how this information and knowledge of the Human Genome might be used to
help reduce the spread of HIV.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
54.
The black rhinoceros, Diceros bicornis, is an endangered species whose numbers

have fallen to approximately 3000 in the past thirty years. For this reason, the species
was placed on Appendix I of the Convention on International Trade in Endangered
Species (CITES) agreement. Since the black rhinoceros has been placed on the
appendix, numbers have stabilised, or even increased, in several countries.
(a)
(i)
Explain the term endangered species.

................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(ii)
Suggest two reasons why the black rhinoceros is endangered.

1 .............................................................................................................
................................................................................................................
2 .............................................................................................................
................................................................................................................
[2]
(b)
State two ways in which the CITES agreement is helping to save endangered
species, such as the black rhinoceros.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 6 marks]
55.
Outline the potential benefits to agriculture of maintaining the biodiversity of wild

animals and plants.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 4 marks]
56.
Health Milk and Energy Boost are flavoured milk drinks.

The manufacturers make the following claims:
HEALTH MILK
Flavoured with real fruit extract.
No added sugar.
ENERGY BOOST
A delicious milk drink packed full of
energy.
Convenient, quick and easy.
The two different flavoured milk drinks and a sample of fresh milk were all tested for the
presence of some biological molecules.
The methods used and the results obtained are shown in the table below.
colour change observed for
method used
a few drops of iodine
solution added
5 cm3 biuret solution added
5 cm3 Benedicts reagent
added and solution boiled
sample that has been

tested with Benedicts
reagent is filtered
the filtrate (solution) is

boiled with 5 cm3 dilute
acid, cooled and
neutralised
(a)
fresh milk
Health Milk
Energy Boost
remains yellow
remains yellow
remains yellow
blue to lilac
blue to lilac
blue to lilac
blue to green
blue to green to
yellow
blue to green to
yellow to orange
remains blue
blue to green to
yellow to orange
blue to green to
yellow to orange
to red
then 5 cm3 Benedicts

reagent is added and the
solution is boiled
Using only the information in the table, state the biological molecules present in
(i)
fresh milk;
................................................................................................................
................................................................................................................
[2]
(ii)
Health Milk.
................................................................................................................
................................................................................................................
[3]
(b)
What differences between Health Milk and Energy Boost are identified by
the information in the table?
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(c)
Explain why the claims made by the manufacturer for Health Milk could be
misleading.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(d)
Suggest why it would not be appropriate to test milk for lipids using the emulsion
test.
.........................................................................................................................
.........................................................................................................................
[1]
[Total 11 marks]
57.
The diagram below is an electron micrograph of part of a cell from a human liver.
This cell is responsible for converting glucose in the body into glycogen for storage.
The glycogen can be seen as granules in the cytoplasm.
(i)
Describe the molecular structure of glycogen.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
(ii)
Name the type of chemical reaction that takes place during the formation of
glycogen.
.........................................................................................................................
[1]
[Total 5 marks]
58.
The formation of glycogen is one of many enzyme-controlled reactions carried out by

liver cells in humans. The liver is a very active organ and generates a lot of heat. The
temperature must not be allowed to increase too much as it will affect the rate at which
glucose is converted into glycogen.
(i)
Suggest the optimum temperature for these enzyme-controlled reactions.

.........................................................................................................................
[1]
(ii)
A significant increase in temperature above the optimum has an effect on the

rate of an enzyme-controlled reaction.
Explain why this is so.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 5 marks]
59.
DNA is found in the nucleus of a cell.
During interphase DNA replicates.
DNA is involved in the transcription stage of protein synthesis.
The following statements, A to H, refer to events that may take place during:
DNA replication only
transcription only
both DNA replication and transcription
neither DNA replication nor transcription.
Complete the table by marking the appropriate boxes with a tick ( ) if the event takes
place or a cross ( ) if it does not take place.
DNA replication
A
Nucleotides line up along an exposed

DNA strand.
The whole of the double helix unzips.
Uracil pairs with adenine.
A tRNA triplet pairs with an exposed

codon.
Both DNA polynucleotide chains act

as templates.
Adjacent nucleotides bond, forming a

sugar-phosphate backbone.
The original DNA molecule is

unchanged after the process.
Adenine pairs with thymine.
transcription
[Total 8 marks]
60.
Over the last few years there has been much public concern over the diet of people in
the UK and its effects upon their weight and health.
Body Mass Index is a calculation used by doctors to indicate whether a person is
underweight or overweight.
(a)
State the medical term used to describe a person whose Body Mass Index is
greater than 30.
.........................................................................................................................
[1]
The table below shows the daily intake of certain components in three diets,
A, B and C for men in the UK.
Diet A
a normal balanced diet for a typical man
Diet B
a weight-reducing low fat diet

restricted to avoid fats
includes any fruit, vegetables and proteins
energy intake is monitored carefully
Diet C
a weight-reducing low carbohydrate diet

restricted to avoid carbohydrates
excludes fruit as these contain sugars
includes any non-starchy vegetables, proteins and fats
energy intake is not counted and may exceed 10 000 kJ
on some days
Diet A
normal balanced
diet
energy / kJ
Diet B
weight-reducing
low fat diet
Diet C
weight-reducing
low carbohydrate
diet
9720
6000
8000
fats / g
87
34
124
carbohydrates / g
275
200
20
proteins / g
88
76
165
combined
minerals / g
12
12
18
(b)
In any unbalanced diet it is possible that there may be a deficiency of certain

nutrients.
Suggest one nutrient that may be deficient in diet B and one in diet C.
Diet B ..............................................................................................................
Diet C ..............................................................................................................
[2]
(c)
(i)
Explain which diet, B or C, is likely to cause more rapid weight loss.

................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(ii)
State the relationship between energy intake and energy use that would
allow a person to lose weight.
................................................................................................................
[1]
(d)
Doctors suggested that diet C may not be very healthy in the long term, as it
contains unlimited amounts of fats and no fruit.
Suggest what potential health problems, other than continued weight loss,
might result in a person who kept to a low carbohydrate diet, similar to diet C.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 9 marks]
61.
Read the following passage and complete each sentence by writing the most
appropriate word in the spaces provided.
Health can be defined as a state of complete ....................................., mental and social
well-being. It is not merely the absence of infirmity or ..................................... . Many people
may consider themselves healthy, even though they do not fully match the above criteria. A
young woman can improve her health in a number of ways.
To improve physical well-being she should eat a balanced diet in which the majority of her
energy needs come from ...................................... Her diet should include only small
quantities of ..................................... fats and more plant oils, such as olive oil. She should
exercise for at least ..................................... minutes on three or more occasions per week.
This exercise should be at an intensity that raises her heart rate to .....................................
percent of her maximum heart rate. She should not smoke at all and should avoid passive
smoking.
[Total 6 marks]
The diagram below shows the incidence of coronary events and mortality from
coronary heart disease (CHD) in some countries around the world. The incidence of
coronary events refers to the number of reported cases of any form of coronary illness
per 100 000 of the population.
The diagram also shows the prevalence of smoking in the same countries. The
prevalence of smoking is the percentage who smoke cigarettes every day.
The figures in the diagram refer to men aged from 35 to 64 during the late 1990s.
Key:
incidence
of coronary
events and
mortality from
CHD /
no. per 100 000
incidence of coronary events / no. of cases per 100 000

mortality from CHD / no. per 100 000
prevalence of smoking / %
900
90
800
80
700
70
600
60
500
50
400
40
300
30
200
20
100
10
prevalence of smoking / %
62.
0
Australia China
Finland Germany Russia Scotland
USA
Source: British Heart Foundation Health Promotion Research Group www.heartstats.org
(a)
The diagram above shows that the relationship between the incidence of
coronary events and the mortality from CHD is not the same in all the countries
shown.
Suggest three reasons why this relationship is not the same.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
3 ......................................................................................................................
.........................................................................................................................
[3]
(b)
Using the information in the diagram, describe the evidence to suggest that
smoking is not the only factor involved in causing heart disease.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(c)
Describe three steps a government might take to try to reduce the mortality from
CHD.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
3 ......................................................................................................................
.........................................................................................................................
[3]
[Total 8 marks]
63.
Below is a diagram showing the structure of a typical antibody.

hinge
region
binding
site
light
polypeptide
chain
constant
region
disulphide
bridge
heavy
polypeptide
chain
(a)
Name the type of cell that produces antibodies.

.........................................................................................................................
[1]
(b)
(i)
State one function for each of the component parts listed below.
binding site .............................................................................................
................................................................................................................
disulphide bridge ....................................................................................
................................................................................................................
constant region ......................................................................................
................................................................................................................
hinge region ...........................................................................................
................................................................................................................
[4]
(ii)
Explain why the part of the antibody molecule incorporating the binding site
is often called the variable region.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
[Total 7 marks]
64.
The figure below shows the concentration of antibodies in the blood following a first
infection by a pathogen on day 0.
140
120
concentration 100
of antibodies / 80
arbitrary units 60
40
20
0
0
10
20
30
40
50
60
time / days
70
80
90
100
(i)
Explain why there is a delay between the first infection by the pathogen and the
appearance of antibodies in the blood.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
On the figure above, draw a curve to show the expected concentration of

antibodies in the blood following a second infection of the same pathogen at
day 30.
[2]
[Total 4 marks]
65.
Antibiotics can be used to artificially prevent bacterial infections. However, resistance

to antibiotics is common among bacteria. For example, the so-called superbug MRSA
(methicillin-resistant Staphylococcus aureus) is resistant to many antibiotics.
(i)
State the way in which a bacterium develops resistance to an antibiotic.

.........................................................................................................................
[1]
(ii)
Suggest two measures, apart from use of antibiotics, that could be taken in a
hospital to combat possible infection with MRSA.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 3 marks]
66.
The figure below shows some deadwood that has been colonised by fungi.
(i)
List three features of organisms belonging to the Kingdom Fungi.

1 ......................................................................................................................
2 ......................................................................................................................
3 ......................................................................................................................
[3]
(ii)
State two features that fungi have in common with plants.

1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 5 marks]
67.
Malaria is caused by the parasite Plasmodium which is a single-celled protoctist. At

one stage in the life cycle the parasites reproduce asexually within human red blood
cells.
(i)
Describe how Plasmodium reproduces asexually.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
State two advantages of asexual reproduction to a parasite such as Plasmodium.

1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
68.
In order to reproduce asexually, Plasmodium needs to obtain amino acids from red
blood cells of its host.
Suggest how Plasmodium obtains amino acids from haemoglobin within red blood
cells.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
In Finland, a national campaign in 1988 led to a reduction in the use of the antibiotic
erythromycin.
The figure below shows the number of doses of erythromycin used per thousand
people per month over the eleven year period 198494.
The percentage of infections each year caused by the bacterium Streptococcus
pyogenes that were resistant to erythromycin is also shown.
key:
doses of erythromycin
percentage of bacterial infections resistant to erythromycin
3.0
percentage
of
bacterial
infections
resistant to
erythromycin
doses of
erythromycin
per
2.5
thousand
people per
month
year
1994
1993
0.0
1992
1991
0.5
1990
10
1989
1.0
1988
15
1987
1.5
1986
20
1985
2.0
1984
69.
(i)
With reference to the information in the figure, explain the changes in the
percentage of infections of S. pyogenes that are resistant to erythromycin.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
(ii)
Describe two ways in which resistance to erythromycin may arise in a population

of S. pyogenes.
1 ......................................................................................................................
.........................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 8 marks]
70.
The table below shows data from research investigating the presence of pesticide
(insecticide and herbicide) residues in drinking water in the UK from 1995 to 1998.
number of samples
analysed over 4 years
% of samples over
safe limit for drinking
lindane
2227
0.7
DDT
1057
0.2
mecoprop
2281
27.9
333
10.2
pesticide
insecticide
herbicide
atrazine
Data from Guide to a Green Planet, edited by Jules Pretty, pp.7175, University of Essex, 2002
(a)
DDT was banned from use in the UK in 1986.

Suggest why DDT was detected in drinking water more than ten years following
the ban.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(b)
The table above shows that a greater percentage of samples analysed for
herbicide residues were over the safe limit than those analysed for insecticide
residues.
Suggest why farmers used larger quantities of herbicides than insecticides.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 5 marks]
71.
Aphids are pest species which farmers control using insecticides. It is important that
farmers use the correct dose of the active ingredient in an insecticide on their crops to
ensure effective control. The following table shows doses that a farmer would apply to
a crop of barley to control aphids.
active ingredient
deltamethrin
dimethoate
tau-fluvalinate
-cyhalothrin and pirimicarb
chlorpyrifos
dose / cm3 ha1

250
845
150
1000
700
Data from Guide to a Green Planet, edited by Jules Pretty, pp.7175,

University of Essex, 2002
Using the table state which active ingredient is the most toxic to aphids and explain
your answer.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total: 2 marks]
72.
The Millennium Seed Bank Project (MSBP) is a global conservation programme

conceived, developed and managed by the Seed Conservation Department at the
Royal Botanic Gardens, Kew. The aims of the project are to collect and conserve 10%
of the worlds seed-bearing plants by 2010.
The project aims to make seeds available for research and species re-introduction into
the wild. Scientists working in seed banks have to maintain the viability and genetic
variability of the seeds they store. Samples of seeds stored are germinated to assess
their variability.
Describe how scientists working in seed banks maintain the viability and genetic
variability of seeds.
viability .....................................................................................................................
..................................................................................................................................
..................................................................................................................................
genetic variability .....................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
73.
In this question, one mark is available for the quality of spelling, punctuation and
grammar.
Zoos and botanic gardens, such as Kew Gardens, are involved in many conservation
projects throughout the world.
Outline the problems experienced by zoos and botanic gardens in managing such
projects and explain why it is important for such projects to be successful.
[7]
Quality of Written Communication [1]
[Total 8 marks]
74.
The effect of diet on cancer mortality is partly due to its effect on obesity. Obesity is
defined as having a Body Mass Index (BMI) over 24.9. BMI is calculated by the
following formula.
BMI =
body mass (kg)

height (m)
The figure below shows the effect of BMI on the relative risk of dying from cancer. Nonobese people with a BMI of 18.524.9 are assigned a baseline risk of 1.
2.0
men
women
relative
risk
of death
from cancer
1.5
1.0
0.5
0.0
18.5-24.9
25.0-29.9 30.0-34.9 35.0-39.9
40.0
BMI
National Obesity Forum, Reproduced by kind permission of the National Obesity Forum
(i)
Calculate the BMI of a woman 1.7 m tall with a mass of 105 kg.
Answer = .........................................................
[2]
(ii)
Use the figure above to explain the likelihood of this woman dying from cancer.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
75.
The table below shows six statements that apply to biochemical tests.
Complete the table to show which of these statements apply to the biochemical tests
carried out on the substances listed.
Fill in each box using a tick ( ) to show that the statement applies or a cross (
does not. The first row has been completed for you.
) if it
statement
substance
use heat
use biuret
reagent
use
Benedicts
reagent
boil with a
dilute acid
a positive
result is a
blue-black
colour
a positive
result is an
emulsion
lipid
protein
starch
reducing sugar
non-reducing
sugar
[Total 4 marks]
76.
A sucrose molecule is a carbohydrate molecule made by joining a glucose unit to a

fructose unit.
(i)
Name the bond that joins the units in a molecule of sucrose.

.........................................................................................................................
[1]
(ii)
Name the type of reaction that breaks this bond.

.........................................................................................................................
[1]
[Total 2 marks]
77.
Sucralose is a chemical that is similar in structure to sucrose. It has been made from
sucrose by replacing three of the OH (hydroxyl) groups with Cl (chlorine) atoms.
The diagram below shows a molecule of sucralose.
CH2OH
Cl
H
CH2Cl
O
H
H
OH
O
H
OH
OH
O
H
OH
CH2Cl
The following claim is made for sucralose:

Sucralose has the same sweet taste as sucrose.
It cannot be digested by enzyme action in the human body and so it
does not lead to weight increase.
Using the information in the diagram to help you, suggest why sucralose cannot be
digested in the body.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 4 marks]
78.
The table below shows a number of categories of disease, a definition of each category
and one example of a disease that fits into each category.
Complete the table.
category
definition of disease category
one example
deficiency
diseases caused by poor diet
scurvy
.................................
diseases caused by a genetic fault

passed from the parents
cystic fibrosis
...............................................................
degenerative
...............................................................
Huntingtons disease
...............................................................
infectious
diseases that are transmitted by a

pathogenic organism which invades the
body
.................................
diseases caused by changes to the mind
physical
...............................................................
...............................................................
................................
schizophrenia
asthma
[Total 5 marks]
79.
Epidemiology is the study of patterns of disease and the factors that affect their
occurrence and spread.
State three ways in which members of the medical profession can use information
about how diseases spread.
1 ...............................................................................................................................
..................................................................................................................................
2 ...............................................................................................................................
..................................................................................................................................
3 ...............................................................................................................................
..................................................................................................................................
[Total 3 marks]
80.
The different parts of the gaseous exchange system, such as the bronchi, show
structural adaptations to their functions. The diagram below shows a section through
the wall of a bronchus as seen with a light microscope.
(a)
(i)
State one function for each of the following components of the bronchus
wall.
goblet cell ...............................................................................................
................................................................................................................
cartilage .................................................................................................
................................................................................................................
[2]
(ii)
State two ways in which the structure of the wall of the bronchus would be
different in a long-term smoker.
1 .............................................................................................................
................................................................................................................
2 .............................................................................................................
................................................................................................................
[2]
(b)
Gaseous exchange occurs across the walls of the alveoli.

Explain why the walls of the alveoli contain elastic fibres.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(c)
One feature of the disease emphysema is that the alveoli lose their elasticity.
Explain the effects of this loss of elasticity on the gaseous exchange system of a
person with emphysema.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 10 marks]
81.
The diagram below shows an artery lying on the surface of living heart muscle as seen
by an instrument called an endoscope. The lumen of the artery has become narrowed
at the point labelled Y.
The Forum on Ischaemic Heart Disease.

Reproduced by kind permission of Dr Graham Jackson,
Cardiology Unit, Guys and St Thomas Hospital.
(i)
Name the artery shown in the diagram.

.........................................................................................................................
[1]
(ii)
Explain how the lumen of the artery has become narrowed at point Y.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 3 marks]
82.
(i)
Suggest how doctors might treat a patient with narrowing of the arteries that
supply the heart muscle.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
Suggest two pieces of advice that a doctor might give to such a patient to try to
reduce the likelihood of further narrowing of the arteries.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
83.
(a)
Milk contains a number of important nutrients including:
proteins which contain amino acids
fats which contain fatty acids
minerals.
Name two other groups of nutrients found in a balanced diet.

1 ......................................................................................................................
2 ......................................................................................................................
[2]
(b)
Some amino acids are known as essential amino acids.

(i)
State what is meant by the term essential amino acids.

................................................................................................................
................................................................................................................
[1]
(ii)
Outline the functions of essential amino acids in the body.

................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(c)
The photograph below shows a child with kwashiorkor, a form of protein energy
malnutrition.
Reproduced by kind permission of Tom D Thacher,

MD, www.thachers.org
(i)
Describe the symptoms of kwashiorkor.

................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[3]
(ii)
Explain why the onset of this disease often occurs between the ages of six
months and eighteen months.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
[Total 10 marks]
84.
The table below shows:
the percentage of people with HIV/AIDS in different regions of the world at

the end of 2002
the number of new infections with HIV between 1999 and 2002 expressed
as a percentage of those with HIV/AIDS at the end of 2002.
region
percentage of people
with HIV/AIDS at the
end of 2002
number of new infections

between 1999 and 2002 as
% of those with HIV/AIDS at
the end of 2002
East Asia and Pacific
76
Eastern Europe and

Central Asia
15
196
North Africa and

Middle East
74
North America
16
15 39
52
15
Sub-Saharan Africa
Western Europe
It has been suggested that HIV/AIDS is a greater problem in less economically

developed regions than in more economically developed regions.
Describe the evidence in the table that supports this suggestion.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
85.
grammar.
Explain why it is difficult to prevent the spread of HIV/AIDS and explain why the
increase in the number of cases is so much higher in some parts of the world than in
others.
[7]
[Total 8 marks]
86.
(a)
Complete the following passage.

In October 2004, scientists announced successful trials of a malaria vaccine. The
vaccine was developed from proteins taken from the parasite, ......................................
falciparum. When the proteins enter the body they act as ......................................
which are recognised as foreign by the immune system. These foreign proteins activate
lymphocytes called T cells which then divide to increase in numbers. Some of these
newly cloned cells become ...................................... cells which attack infected cells in
the liver. Others become ...................................... cells which release a hormone-like
messenger molecule called a ...................................... . These molecules activate B
cells to divide and produce plasma cells. The B cells also produce
...................................... cells that stay in the body for a number of years. The result is
an immune system prepared to make a strong attack on the parasite when it enters the
body.
[6]
(b)
Name the molecules, released by plasma cells, that attack the parasite when it
enters the body.
.........................................................................................................................
[1]
(c)
Suggest why it has been difficult to produce a malaria vaccine.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 10 marks]
87.
(a)
The malarial parasite, Plasmodium, and its vector, the mosquito, are both
eukaryotes.
The treatment and control of malaria is difficult because Plasmodium rapidly
develops resistance to most anti-malarial drugs as do mosquitoes to insecticides.
Also, vaccine production has proved to be very difficult. The B-cell responses
induced by experimental vaccines are not yet very effective.
Explain
(i)
the genetic basis of resistance in eukaryotes;

................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[5]
(ii)
why producing an effective vaccine against Plasmodium has proved to be

so difficult.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[3]
(b)
A gene has been identified in several species of Plasmodium which codes for a
small transmembrane protein.
A mutant form of P. berghei exists in which this protein is not produced. P.
berghei infects mice. The mutants:
(i)
develop normally in a mosquito and infect the salivary glands in numbers

comparable to wild type parasites
infect mouse liver cells but do not multiply
do not infect red blood cells.
Describe one mutation of this gene that could have occurred in P. berghei
so that the encoded protein is not produced.
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(ii)
Suggest one reason why mutant P. berghei do not infect red blood cells.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(c)
It has been suggested that Plasmodium with this mutation could be used as a
whole organism vaccine against malaria.
Mice were inoculated with different numbers of mutant Plasmodium and then
given one or two booster inoculations. Their protection against infection by wildtype Plasmodium was compared with that of mice that had not been inoculated.
The results of the investigation are shown in the table below.
number of mutant Plasmodium
in initial inoculation
in first booster
inoculation
in second booster
inoculation
percentage of mice
resistant to infection by
wild-type Plasmodium
50 000
25 000
25 000
100
10 000
10 000
10 000
100
10 000
10 000
70
With reference to the information in the table and in (b), comment on the use of
this mutant Plasmodium as a whole organism vaccine.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total: 15 marks]
88.
Musk deer occur throughout forested mountain habitats in Asia and eastern Russia.
They live in small groups, normally three individuals in a group, and are primarily active
at night.
The deer are hunted illegally for traditional medicine and also threatened by habitat
destruction. Populations of musk deer in China and Mongolia are listed in Appendix II
of the Convention for International Trade in Endangered Species (CITES).
Explain what is meant by the term endangered species.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
89.
In this question, one mark is available for the quality of the use and organisation of
scientific terms.
Musk deer populations in isolated habitats could be conserved by a programme of
captive breeding. Zoos, such as London Zoo and Jersey Zoo, are involved in captive
breeding of many endangered species.
Describe how a captive breeding programme for musk deer would be set up and
discuss the problems associated with the reintroduction of the captively bred deer back
into the wild.
[7]
[Total 8 marks]
90.
Hedgerows are important in farming as they act as sites of refuge for beneficial insects,
provide protection for the crop from adverse weather conditions and act as wildlife
corridors.
Farmers are advised to leave strips of land between hedgerows and the crops in the
fields to encourage biodiversity.
Describe how you would investigate whether leaving strips of land around fields
encourages plant biodiversity.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 5 marks]
91.
When hedgerows are destroyed there is a loss of biodiversity.

Suggest why this loss of biodiversity is of concern.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total: 3 marks]
92.
The Royal Society for the Protection of Birds (RSPB) and the British Trust for
Ornithology (BTO) carried out research into the declining populations of farmland bird
species. In a study carried out from 1970 to 1998 they found that farmland bird species
had decreased by up to 68% across rural areas of the UK.
(i)
Changes in farming practices, such as the increased use of pesticides, are

highlighted by the RSPB and BTO as possible causes of this decline in bird
species.
Suggest how the increased use of pesticides could have caused this decline.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
An increase in the number of predators, such as magpies and sparrowhawks,

has also been suggested as a possible cause for the decline in the populations of
farmland bird species.
Suggest why this may not be the cause.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 5 marks]
93.
grammar.
The immense biodiversity of the oceans includes:
corals that host symbiotic algae which die if the sea temperature rises by 1C;
animals like polar bears that use floating ice as a base for sea fishing
expeditions;
sessile animals like mussels that feed by filtering food particles from the water
and reproduce by releasing gametes into the water;
reef-building animals like corals that form hard calcium carbonate skeletons by
extracting mineral ions such as Ca2+ from the water;
seaweeds of different colours which occur in shallow water;
animals like fish that hunt prey using well-developed visual skills;
large animals such as the blue whale;
physically delicate organisms like jellyfish that lose their shape in air.
Using examples from the list above, describe and explain how the properties of water
make it a suitable environment for these organisms.
[7]
[Total 8 marks]
94.
Two students carried out an investigation into the effect of pH on the activity of a
lysosomal enzyme. Student A drew the graph shown in the diagram below.
50
40
rate
of 30
enzyme
activity \
arbitrary
units
20
10
0
1
pH
(i)
A teacher asked two students to state the optimum pH for this enzyme. Student A
gave the answer pH 3.25 but student B gave the answer somewhere between
pH 3.0 and pH 4.3. The teacher said that student B had given the better answer.
Explain why student Bs answer was better.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
Explain why this enzyme is not active at pH 7.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
95.
grammar.
Explain the effects of enzyme concentration, substrate concentration and competitive
inhibitors on the rate of an enzyme-controlled reaction.
[9]
[Total 10 marks]
96.
Read the following passage and then answer the questions that follow.
Human Factor VIII is a glycoprotein found in blood plasma. It is involved in blood
clotting.
This glycoprotein contains 2332 amino acids linked into a single chain. This chain
is folded and coiled into a secondary structure and then further folded. The chain
forms six individual regions, each with its own function.
An artificial source of Factor VIII, created using genetic engineering, is now used
to treat patients with haemophilia, a medical condition in which the blood clots
more slowly than normal. The Factor VIII gene is first removed from the genome
of human cells. It is then inserted into the genome of hamster cells.
10
Cancer cells or cells taken from an ovary are usually used to produce Factor VIII
as these grow very well in industrial tanks. The Factor VIII that is produced is
then removed from the tanks and purified before use in treating patients.
(a)
State what is meant by the term glycoprotein (line 1).

.........................................................................................................................
.........................................................................................................................
[1]
(b)
The secondary structure of a protein is identified by its shape.

(i)
Name a shape formed by coiling of the primary structure.

................................................................................................................
[1]
(ii)
Name a shape formed by folding of the primary structure.

................................................................................................................
[1]
(c)
State the name given to the level of structure formed by further folding of the
secondary structure (line 4).
.........................................................................................................................
[1]
[Total 4 marks]
97.
Complete the following passage by using the most suitable word(s) in each of the blank
spaces.
Water is essential for life. It makes up a high proportion of the cytoplasm in a cell. Many
different compounds can dissolve in it and it is therefore described as an excellent
..................................... .
Water remains in the ....................... state over a wide range of environmental
temperatures. As it cools below 4 C it becomes less ............................. than warmer
water. Ice floats on water, forming a layer that ................................... the water beneath
with the result that large bodies of water rarely freeze entirely.
The ............................... bonds that form between water molecules are responsible for
its high ............................................................. , which allows small insects such as
pond skaters to move on its surface without sinking.
[Total 6 marks]
98.
The Human Immunodefficiency Virus (HIV) is spread by exchange of body fluids

between an infected person and an uninfected person. This often occurs as a result of
unprotected sexual intercourse. HIV / AIDS is categorised as an infectious disease.
Listed below are three other categories of disease.
(a)
State one example of a disease that fits into each category.

mental disease ...............................................................................................
self-inflicted disease .......................................................................................
inherited disease ............................................................................................
[3]
The diagram below shows the percentage of people infected with HIV in different parts
of the world at the end of 2002.
North America
0.5 - 1%
Caribbean
1 - 5%
Latin America
1 - 5%
Western Europe
0.5 - 1%
Eastern Europe
& Central Asia
1 - 5%
East Asia & Pacific
0.5 - 1%
North Africa & Middle East

0.5 - 1%
Sub-Saharan Africa
15 - 39%
South &
South-East Asia
0.5 - 1%
Australia &
New Zealand
0.1%
data from UNAIDS
(b)
(i)
Explain why it is useful to collect information, such as that shown in the

diagram.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[3]
(ii)
The percentage of people infected with HIV is much higher in Sub-Saharan

Africa than in much of Europe.
Suggest why the percentages are so much higher in Sub-Saharan Africa.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(c)
At present there is no cure for HIV/AIDs. Efforts to reduce the spread of HIV
infection are centred on reducing the chances of a person carrying HIV passing it
on to others.
Suggest how information gained from the Human Genome Project might be used
to help reduce the spread of HIV.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 10 marks]
99.
The diagram below shows stages in the immune response to invading bacteria.
1
bacteria engulfed by cells from the immune system
removal of antigens from bacteria
antigens presented on surface of

engulfing cells
correct T helper cells are selected
reproduction of T helper cells
release of cytokines
cytokines activate B cells
reproduction of B cells
some B cell clones

differentiate to make
plasma cells
10
plasma cells manufacture

antibodies
(i)
Name the type of cell that engulfs the bacteria in stage 1.
some B cell clones

differentiate to make
B memory cells
.........................................................................................................................
[1]
(ii)
Suggest how the antigens are removed from the bacteria in stage 2.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(iii)
Explain how the correct T helper cells are selected in stage 4.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(iv)
Name the type of cell division used for reproduction of the T helper cells in
stage 5.
.........................................................................................................................
[1]
(v)
Explain the importance of B memory cells in immunity.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 10 marks]
100. Describe how antibodies act on invading pathogens, such as bacteria or viruses.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
101. Both Fig. 1 and Fig. 2 are photographs of lung tissue taken through a light microscope
at the same magnification.
Fig. 1
John Burbridge / Science Photo Library
Fig. 2
Fig. 1 shows healthy lung tissue.
Fig. 2 shows lung tissue damaged by tuberculosis.
(a)
Name the organism that causes tuberculosis.

.........................................................................................................................
[1]
(b)
Suggest how damage to lung tissue in tuberculosis, as seen in Fig. 2, is likely to

affect a person with this disease.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(c)
Outline the reasons why tuberculosis has not been eradicated.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[5]
[Total 8 marks]
102. (a)
Name two diseases that may be caused by many years of cigarette smoking.
1 ......................................................................................................................
2 ......................................................................................................................
[2]
(b)
In this question, one mark is available for the quality of use and organisation of
scientific terms.
The diagram below shows three components inhaled as a result of smoking a
cigarette.
carbon monoxide
nicotine
tar
Describe the effects of these three components of cigarette smoke on the body.
[8]
[Total 11 marks]
103. The tiger, Panthera tigris, is the largest and most distinctive cat in the world.
Complete the following table to show the classification of the tiger.
kingdom
.....................................................
.....................................................
chordata
.....................................................
mammalia
order
carnivora
family
felidae
genus
.....................................................
.....................................................
Panthera tigris
[Total 5 marks]
104. Haemolytic disease of the newborn can occur if red blood cells are broken down too
rapidly.
This is caused by antibodies, produced by the mother, crossing the placenta into the
fetal circulation.
Suggest how antibodies cause the breakdown of red blood cells in haemolytic disease
of the newborn.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
105. Seed banks maintain the genetic diversity of plant populations.

State two methods used to preserve seeds in seed banks.
1 ...............................................................................................................................
2 ...............................................................................................................................
[Total 2 marks]
106. When Darwin first came to the Galapagos there were some 3000 tortoises which had
been reduced to 14 by 1965. Since then each type of tortoise has been kept on its own
island as part of a captive breeding programme. The Espanola Tortoise was
successfully bred so that there are now about 1000 of them on Santa Cruz Island.
Conservationists are now planning to release them into the wild on another island to
increase the population there.
(a)
Suggest two reasons why the population decreased before 1965.

1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
Captive breeding programmes are not always as successful as the one described
above.
(b)
Explain why many species do not breed successfully in captivity.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(c)
State two problems encountered when releasing captive bred individuals into the
wild.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
(d)
A further study of 134 individuals was carried out on the tortoise population. It
was found that the population had a very low genetic diversity. This was caused
by an introduced male tortoise from the San Diego Zoo who fathered nearly 80 of
the individuals sampled.
Suggest how a low genetic diversity may be damaging to the population.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 10 marks]
107. Cholesterol is a lipid which forms part of the structure of membranes of animal cells. It
is absorbed from food and can also be synthesised by liver cells.
Cholesterol is transported by the blood with the help of specific transport proteins to
which cholesterol molecules become reversibly attached. These complexes of lipid and
protein are known as lipoproteins. There are three different types of lipoprotein
transporting cholesterol in the blood. The concentration of cholesterol in blood can be
measured, either as the total cholesterol, or as the amount carried by each of the
different types of lipoprotein.
Explain why cholesterol must be carried in the blood by proteins while glucose does
not need any transport protein.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
108. Researchers and medical practitioners calculate the ratio of the total blood cholesterol
concentration (TC) to the concentration of cholesterol carried by one type of lipoprotein,
called high density lipoprotein (HDL). This ratio is called the TC : HDL ratio.
The TC : HDL ratio and the resting systolic blood pressure are both factors which are
associated with the risk of having coronary heart disease (CHD).
Systolic pressure is the pressure in the major distributing arteries when the left ventricle
contracts.
The way in which both the TC : HDL ratio and the resting systolic blood pressure are
associated with the risk of CHD, is shown in the figure below.
key
less than 15% probability of developing coronary heart disease in the
next ten years
15% to 30% probability of developing coronary heart disease in the
next ten years
more than 30% probability of developing coronary heart disease in the
next ten years
24.0
21.5
resting
systolic blood
pressure / kPa
19.0
16.5
14.0
3
7
TC : HDL ratio
11
Using only the information above, describe the influence of the TC : HDL ratio and the
resting systolic blood pressure on the risk of developing CHD.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
109. The table below shows information about tests that identify three different types of
biological molecule.
Complete the table to show the names of the types of molecule that are tested, the
reagents used and the results obtained.
type of molecule tested
reagents used
positive result
negative result
................................
protein
...............................
................................
blue solution
...............................
................................
.................................
starch
alcohol and water

...............................
white emulsion
clear liquid
...............................
yellow solution
................................
[Total 5 marks]
110. A student followed a procedure to find the concentration of reducing sugars in a fruit
juice. The first part of the method used was as follows:
A range of glucose solutions of different concentrations was made up, starting

with a 20 g dm3 glucose solution.
Each solution was boiled with excess Benedicts solution.
When there was no further change in colour, the liquid was cooled and filtered.
The absorbance of the liquid was measured with a colorimeter. (A colorimeter
measures the amount of light that is absorbed by a coloured solution.)
The students results are shown in the diagram below.

1.0
0.9
0.8
0.7
absorbance /
arbitrary units
0.6
0.5
0.4
0.3
0.2
0.1
0.0
0
(i)
5
10
15
glucose concentrationg/ dm3
20
State two precautions that the student should have taken during the procedure to
ensure that the results give a valid comparison between the different glucose
solutions.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
(ii)
In the second part of the method, the student tested the fruit juice. The
absorbance reading obtained was 0.60 arbitrary units.
Use the diagram above to determine the reducing sugar concentration of the fruit
juice.
.............................................................................................................. g dm3
[1]
(iii)
This procedure does not test for non-reducing sugars, such as sucrose.
How should the procedure be altered to determine the concentration of nonreducing sugar in the fruit juice?
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 5 marks]
111. The diagram below is a drawing of an alveolus together with an associated blood
capillary.
Oxygen diffuses from the alveolus into cell X. Cell X carries oxygen around the body in
the blood stream.
(i)
Name the compound inside cell X that combines with oxygen.

.........................................................................................................................
[1]
(ii)
Name the metal ion required for the formation of the compound in (i).
.........................................................................................................................
[1]
[Total 2 marks]
112. An experiment was carried out in which the enzyme lipase was used to hydrolyse a
triglyceride. The pH of the reaction mixture was recorded at regular intervals during the
experiment. The results are shown in the table.
(i)
time / min
pH
7.0
6.2
5.6
5.1
4.7
10
4.6
12
4.6
14
4.6
State what is meant by the term hydrolysis.

.........................................................................................................................
.........................................................................................................................
[1]
(ii)
Explain why the pH falls during the reaction.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(iii)
After 14 minutes, the mixture was analysed and unreacted triglyceride was found
to be present. No inhibitor was added to the reaction mixture.
Explain why the reaction had stopped after 10 minutes.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 5 marks]
113. Explain how a non-competitive inhibitor affects the rate of an enzyme-catalysed

reaction.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
114. During research into the mechanism of DNA replication, bacteria were grown on a
medium containing nitrogen isotopes. The nitrogen isotopes used were heavy
nitrogen, 15N, and light nitrogen, 14N. After growth, the bacterial DNA was isolated
from the cells and spun in a centrifuge. The DNA settled in the centrifuge tube at a
position that corresponded to its density, indicating the proportion of the different types
of DNA present in the sample.
Bacteria were grown for many generations in a medium containing only the heavy
isotope of nitrogen, 15N. This resulted in all the DNA molecules containing only 15N.
The result after centrifugation is shown in Fig. 1.
These bacteria were then grown in a medium containing only light nitrogen, 14N. After
allowing time for the DNA to replicate once, the DNA was analysed as before. The
result is shown in Fig. 2.
centrifuge
tube
14
14
14
14
N DNA
N/ 15 N DNA
15
N DNA
N DNA
N/ 15 N DNA
15
N DNA
key:
Fig. 1
(a)
DNA
Fig. 2
Explain how this information supports the semi-conservative hypothesis of DNA

replication.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
The bacteria were allowed to continue to grow in the light nitrogen, 14N, until the DNA
had replicated once more. The DNA was analysed as before and the result is shown in
Fig. 3.
14
N DNA
N/ 15 N DNA
15
N DNA
14
Fig. 3
Fig. 4 shows simple diagrams of DNA molecules, indicating the nitrogen content of
each.
Key:
represents
DNA with 15N
C
represents
DNA with 14N
Fig. 4
(b)
Select the letter or letters from Fig. 4 representing the bacterial DNA in Fig. 1,
Fig. 2 and Fig. 3.
Fig. 1 ...............................................................................................................
Fig. 2 ...............................................................................................................
Fig. 3 ...............................................................................................................
[3]
(c)
The bacteria were allowed to continue to grow in the light nitrogen, 14N, until the
DNA had replicated once more. The DNA molecules were analysed as before.
Complete the diagram to indicate the expected results showing the composition
of these DNA molecules.
14
N DNA
14
15
N DNA
15
N DNA
[2]
[Total 9 marks]
115. Smoking is considered to be a disease. In 2004, in the UK, the Wanless Report
recommended a change in emphasis in the way that the National Health Service
tackles certain diseases, such as smoking and obesity.
(a)
Name one category of disease which includes smoking and obesity.

.........................................................................................................................
[1]
(b)
Both these diseases increase the risk of developing coronary heart disease
(CHD). CHD is known as a multifactorial disease.
Suggest what is meant by the term multifactorial disease.
.........................................................................................................................
.........................................................................................................................
[1]
(c)
Explain how cigarette smoke can increase the risk of developing CHD.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(d)
Suggest reasons why the incidence of CHD is not the same in all parts of the
world.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
(e)
The Wanless Report recommended greater emphasis on prevention rather than

cure.
With reference to CHD, suggest what benefits this change in approach may
bring.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 12 marks]
116. A number of definitions are listed in the table below.

In the right hand column, write a term that best matches the definition in the left hand
column. The first one has been done for you.
The type of B cell which secretes antibodies.
plasma cell
The term which refers to any organism that causes

infectious disease.
Diseases which cause a progressive deterioration of part
of the body.
The type of exercise that uses the heart and lungs to
provide oxygen for respiration in muscles.
The volume of air breathed in or out during a single
breath.
A term used to describe a disease that spreads across
continents.
[Total 5 marks]
117. During an immune response, phagocytic cells are attracted to the site of infection.
Describe the action of these phagocytic cells at the site of infection.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 4 marks]
118. Sometimes the immune system overreacts and responds to otherwise harmless
substances. This occurs in allergic reactions.
The diagram below shows the increase in the percentage of children with allergies. The
figures were collected between 1975 and 2005 in the UK.
50
40
% children
with allergies
30
20
10
0
1970
1980
1990
year
2000
2010
(i)
Suggest one reason for the increase shown in the diagram.

.........................................................................................................................
[1]
(ii)
Use the information in the diagram to estimate the percentage of children who
are likely to have an allergy in 2010. Assume the rate of increase remains
constant.
.........................................................................................................................
[1]
[Total 2 marks]
119. An investigation was carried out to determine the effects of increasing carbohydrate
levels in the diet. Consuming extra carbohydrate is a technique called carbohydrate
loading. It is often used by endurance athletes, such as long distance cyclists.
The diagram below shows the effect that different amounts of carbohydrate have on
the length of time an athlete can continue exercising until exhausted.
Clyde Williams (1999) 'Does carbo-loading work?'

Biological Sciences Review vol. 12 No. 2
Using the evidence in the diagram, describe the effect of carbohydrate loading on an
athletes performance.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
120. The diagram below shows the effect that different amounts of carbohydrate have on
the concentration of glycogen stored in the muscles.
Clyde Williams (1999) 'Does carbo-loading work?'

Biological Sciences Review vol. 12 No. 2
Explain why a high concentration of glycogen in the muscles improves an athletes

performance in an endurance event.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
121. (i)
Name the virus that leads to AIDS.

.........................................................................................................................
[1]
(ii)
The virus infects and kills T helper cells in the immune system.
State three ways in which the lack of T helper cells will affect the functioning of
the immune system.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
3 ......................................................................................................................
.........................................................................................................................
[3]
(iii)
State three ways in which this virus can be transmitted from person to person.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
3 ......................................................................................................................
.........................................................................................................................
[3]
[Total 7 marks]
122. The Millennium Seed Bank Project is an international plant conservation project. Its aim
is to prevent the extinction of about 24 000 plant species.
Suggest two benefits of preventing extinction of plant species.
1 ...............................................................................................................................
..................................................................................................................................
2 ...............................................................................................................................
..................................................................................................................................
[Total 2 marks]
123. Papaya fruit are an important commercial crop in many tropical countries. The wild
relatives of C. papaya are found in tropical South America.
(i)
Explain the importance of keeping seeds of the wild relatives of commercial crop
plants, such as papaya, in a seed bank.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
Outline the main steps by which disease resistance could be selectively bred into
commercially grown papaya.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 6 marks]
124. The black rhinoceros, Diceros bicornis, is an endangered species whose numbers
have fallen to approximately 3000 in the past thirty years. For this reason, the species
was placed on Appendix I of the Convention on International Trade in Endangered
Species (CITES) agreement. Since the black rhinoceros has been placed on the
appendix, numbers have stabilised, or even increased, in several countries.
(i)
Explain the term endangered species.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
Suggest two reasons why the black rhinoceros is endangered.

1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
125. The clearing of tropical forests across the Earth has been occurring on a large scale
basis for many centuries. This process, known as deforestation, involves the cutting
down, burning and damaging of forests. The loss of tropical rain forest is more
profound than merely destruction of beautiful areas. If the current rate of deforestation
continues, the worlds rain forests will vanish within 100 years, causing unknown
effects on global climate and eliminating the majority of plant and animal species on the
planet.
State three reasons for the decline in rain forest.
1 ...............................................................................................................................
2 ...............................................................................................................................
3 ...............................................................................................................................
[Total 3 marks]
126. Over half of the species of plants and animals comprising the biodiversity of the Earth
are thought to exist in tropical rain forests.
What are the economic and ethical reasons for maintaining biodiversity?
economic reasons
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
ethical reasons
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 5 marks]
127. In this question, one mark is available for the quality of spelling, punctuation and
grammar.
The figure below shows a green alga, Chlamydomonas, and a cyanobacterium,
Microcystis.
flagellum
Chlamydomonas
contractile vacuole
cell wall
nucleus
starch granule
Microcystis
slime layer
cell wall
DNA
location of
photosynthetic
pigments
2m
Green algae and cyanobacteria (blue-green bacteria) were once classified in the Kingdom
Plantae. As more information became available, taxonomists re-classified these organisms
into separate kingdoms. The green algae are now in the Kingdom Protoctista. Although
cyanobacteria and green algae share certain structural and functional features, they are
placed in different kingdoms.
Suggest why the green algae and cyanobacteria were originally considered to be
plants and explain why taxonomists decided to re-classify them into separate
kingdoms.
Use the information given in the figure above and the box to help you. You may
annotate the figure if you wish.
[7]
[Total 8 marks]
128. Cholesterol molecules are transported in the blood as lipoproteins. Some lipoproteins
are high density lipoproteins (HDLs) and others are low density lipoproteins (LDLs).
The liver regulates the blood cholesterol concentration.
A high blood cholesterol concentration indicates that a persons health may be at risk.
Cholesterol also has many beneficial functions in the body.
Outline the functions of cholesterol in the body.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
129. Suggest why the people were required not to eat before the blood test.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
130. The diagram below represents part of a collagen molecule.
(i)
Collagen is a protein made of three chains of amino acids, twisted together like a
rope. State the name given to a chain of amino acids.
.........................................................................................................................
[1]
(ii)
Name the amino acid that forms a high proportion of the collagen molecule.
.........................................................................................................................
[1]
(iii)
Collagen has tremendous strength, having about one quarter of the tensile
strength of mild steel.
Using information given in the diagram to help you, explain how the structure of
collagen contributes to its strength.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
131. Complete the following passage by inserting the most appropriate terms in the spaces
provided.
Cellulose and collagen are both fibrous molecules. Cellulose, a carbohydrate, is the
main component of the in plants.
Cellulose is made of chains of many glucose molecules which are joined by 1,4
bonds. Each glucose molecule is rotated relative
to its neighbour, resulting in a chain. Adjacent chains are held
to one another by bonds.
[Total 6 marks]
132. Deoxyribonucleic acid (DNA) is a polynucleotide.

(i)
State how many different types of nucleotide are found in DNA.

.........................................................................................................................
[1]
(ii)
Name the components of one of these nucleotides.

.........................................................................................................................
.........................................................................................................................
[3]
[Total 4 marks]
133. DNA replication is described as semi-conservative. Below is a diagram showing the

replication of a DNA molecule.
parent
strands
complementary
new strand
complementary
new strand
Explain what is meant by the term semi-conservative replication.

..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
134. Radiation is one factor that can increase the chance of developing lung cancer.
Radon, a naturally occurring radioactive gas, is found in all rocks and soils. On
average, radon contributes about 50% of the total radiation dose received by people.
Different areas of the UK have different levels of radon because of their rock and soil
types.
Radon seeps out of soils and rocks into the air and may build up in enclosed spaces
such as buildings.
Air containing an average concentration of 20 units of radon is considered to be a
typical figure.
Air containing an average concentration of 200 units has been defined as a Threshold
Level at which radon is considered to be a significant risk.
Research has linked exposure to radon with an increased risk of lung cancer. Smoking
also increases the risk of lung cancer.
Some typical figures are shown in the table below.
estimated risk of developing lung cancer / %
radon level / arbitrary units
no exposure to
cigarette smoke
smoker (15 cigarettes

per day)
0.1
1.0
20
0.1
1.0
200
1.0
10.0
Using the data in the table,

(i)
calculate by how much smoking increases the risk of developing lung cancer;
.........................................................................................................................
.........................................................................................................................
[1]
(ii)
comment on the risks of radon and smoking on the development of lung cancer.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 3 marks]
135. Cyclo-oxidase (COX) is one of the enzymes needed in the formation of prostaglandins,
compounds that are involved in causing fever, pain and inflammation. COX catalyses
the conversion of arachidonate to prostaglandinH2, as shown in Fig. 1.
phospholipid
arachidonate
COX
prostaglandinH2
prostaglandin
Fig. 1
The COX enzyme is found attached to the inner surface of the endoplasmic reticulum
membrane. This is shown in Fig. 2.
Fig. 2
(a)
Using the information given above, suggest why the COX enzyme is found
attached to the inner surface of the endoplasmic reticulum.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(b)
Arachidonate reaches the active site of COX through a hydrophobic channel in

the surface of the enzyme.
Ibuprofen and aspirin are drugs that inhibit the action of the COX enzyme.
Ibuprofen enters and occupies the hydrophobic channel in the surface of

the enzyme.
Aspirin reacts with one of the R groups close to the active site of the
enzyme.
Suggest how each drug inhibits the action of the COX enzyme.
ibuprofen
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
aspirin
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
(c)
Describe the effect of low temperature, such as 5 C, on enzyme action.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 10 marks]
136. The diagram below shows the transmission cycle of the single-celled organism that
causes malaria.
infective stages of parasite
invade mosquito's salivary
glands
cell division produces thousands

of immature malarial parasites
infected mosquito
takes blood meal
male and female gametes
fuse in mosquito's stomach
infective stages
of parasite enter
bloodstream and
then liver cells
liver
parasites leave liver cells

and enter red blood cells
mosquito takes
infected blood meal
maturation and production of parasite's

male and female sex cells (gametes)
Adapted from Fosbery, R. Human Health and Disease, CUP 1997
(a)
Use your knowledge and the information shown in the diagram above to
complete the passage below.
Malaria is caused by a single-celled organism called .................................. . The
organism is transmitted from one person to another by female .............................
mosquitoes. A mosquito takes up the gametes of the malarial parasite when it
feeds on the blood of an ................................... person. Fertilisation occurs in the
mosquitos stomach and the immature parasites reproduce. Infective stages of
the parasite migrate to the mosquitos salivary glands. A new person becomes
infected when the mosquito takes another meal of ................................ . The
parasites enter the liver of the new victim where further reproduction takes place
before migrating to the red blood cells. When an organism, such as the mosquito,
is involved in transmission it is called a ............................. . The malarial parasite
can also be transmitted by .......................... ............................. .
[6]
(b)
Describe two ways in which the transmission cycle of malaria can be disrupted.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 8 marks]
137. Outline how substances such as benzpyrene affect the cells in the lungs.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
138. State two symptoms which could alert someone to the possibility that they may have
lung cancer.
symptom 1 ................................................................................................................
..................................................................................................................................
symptom 2 ................................................................................................................
..................................................................................................................................
[Total 2 marks]
139. Severe Acute Respiratory Syndrome (SARS) is a severe form of viral pneumonia. The
disease was first described in China in November 2002. Within six weeks, 29 countries
were affected. The number of cases in China was considered to be of epidemic
proportions. The World Health Organisation called SARS the first worldwide epidemic
of the twenty-first century.
Vaccination can provide protection against many diseases by inducing artificial active
immunity.
(i)
What is meant by the word artificial in the term artificial active immunity?
.........................................................................................................................
.........................................................................................................................
[1]
(ii)
Describe how an effective vaccine can produce active immunity to a disease.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
(iii)
In 2003, scientists started working to produce a vaccine for SARS.

Explain how vaccination may be used as part of an eradication programme for
diseases such as SARS.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 8 marks]
140. The figure below shows the effect of increasing phosphate concentration on the growth
of three plant species, which commonly grow as weeds amongst crops in the UK.
nettle
dry
mass
small scabious
wavy hair grass
phosphate concentration
Adapted from an article by I.H.Rorison in New Phytologist /
1968 / Volume 67 / Issue 4 / Figure 2b (p.917)
Describe the effects of increasing phosphate concentration on the growth of these

three plant species.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 4 marks]
141. Glucose:
is a carbohydrate
is a hexose (six-carbon sugar)
has the formula C6H12O6
has a six-membered ring structure.
The diagram below shows the molecular structures of two monosaccharide sugars,
glucose and fructose.
CH2OH
H
4
H
OH
OH
H
1
OH
3
O H
glucose
CH2OH
1
2
H
OH
OH
5
6
CH2OH
3
OH
fructose
State one way, visible in the diagram above, in which the structure of fructose is:
similar to glucose;
..................................................................................................................................
..................................................................................................................................
different from glucose.
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
142. Maltose and sucrose are disaccharide sugars in which a bond joins two
monosaccharide molecules. Sucrose is formed by the formation of a bond between
carbon 1 of a glucose molecule and carbon 2 of a fructose molecule.
(i)
Name the bond that joins the two molecules to form a disaccharide.
.........................................................................................................................
[1]
(ii)
Complete the diagram below to show what happens when the glucose and
fructose molecules join together.
CH2OH
H
4
H
OH
OH
H
1
OH
3
O H
CH2OH
1
2
H
OH
5
6
CH2OH
OH
3
OH
[2]
[Total 3 marks]
143. (i)
Describe the test that is used to indicate the presence of a reducing sugar, such
as glucose, and state the observation that would be made if glucose was present.
description of test
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
observation if glucose is present
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
No change is observed if sucrose, a non-reducing sugar, is tested for in this way.

The bond between the glucose and fructose units must first be broken. The test
for a reducing sugar can then be carried out.
Describe how this bond can be broken chemically before carrying out the test for
a reducing sugar.
.........................................................................................................................
.........................................................................................................................
[1]
[Total 4 marks]
144. The diagram below represents an enzyme and a number of other molecules.
A
enzyme
(a)
E
C
Label on the diagram the active site of the enzyme.

[1]
(b)
Write the letter of the molecule that is most likely to be the substrate for this
enzyme.
.........................................................................................................................
[1]
(c)
Use the information in the diagram to explain enzyme specificity.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(d)
One hypothesis of the mechanism of enzyme action is the lock and key
hypothesis. Another hypothesis, the induced fit hypothesis, involves the enzyme
changing shape slightly to allow the substrate to fit perfectly. The substrate also
changes shape slightly.
Suggest how the substrate changing shape slightly will assist enzyme action.
.........................................................................................................................
.........................................................................................................................
[1]
[Total 6 marks]
145. The smoke produced by burning tobacco leaves contains over 4000 different chemical
compounds. Whilst some of these compounds may be harmless, others are addictive
or may cause an increased risk of certain diseases.
(a)
Name one compound in tobacco smoke that is addictive.

.........................................................................................................................
[1]
(b)
Name two other harmful substances found in tobacco smoke. For each
substance describe briefly the nature of the damage caused to the gaseous
exchange system.
substance 1 ....................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
substance 2 ....................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[5]
[Total 6 marks]
146. The diagram below shows the results of a study into the effects of smoking patterns in
different socio-economic groups in the UK.
% heavy smokers in each socio-economic group
number of persons with long-standing illness per 1000 of population
in each socio-economic group
20
500
18
450
16
400
14
350
12
300 number of persons

with long standing
250 illness per 1000 of
200 population
% heavy
10
smokers
8
6
150
100
50
0
1
3
4
socio-economic group
The socio-economic groups shown in the diagram are:

1
2
3
4
5
6
professional, e.g. doctors, teachers

semi-professional, e.g. employers, managers
skilled non-manual, e.g. computer technicians
skilled manual, e.g. plumbers, bricklayers
semi-skilled manual, e.g. painters, decorators
unskilled manual, e.g. labourers, delivery drivers
(i)
With reference to the diagram above, describe the relationship between socioeconomic group and the percentage of heavy smokers.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(ii)
It has been suggested that the proportion of people suffering with long-term
illness in each socio-economic group is directly linked to the percentage of heavy
smokers in each group.
What evidence is there for or against this view?
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(iii)
Suggest two other factors that may contribute to the higher rates of long-term
illness found in the groups of manual workers as compared to non-manual
workers.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 6 marks]
147. Body mass index (BMI) can be used to place people into categories according to their
mass. BMI is calculated by the equation:
BMI =
(a)
body mass in kg
(height in metres) 2
People with a BMI of greater than 30 are classed as obese. State two causes of
obesity.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
As part of a long-term survey into the health of the nation, a random sample of the
English population was selected every five years. The BMI of each member of the
sample was calculated and the percentage of people fitting into each mass category
was recorded. The results are shown in the table below.
year
BMI
category
1980
1985
1990
1995
2000
underweight
12
acceptable
53
51
46
41
36
overweight
28
32
34
36
39
obese
14
17
20
(b)
Describe the trends shown in the table above.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 6 marks]
148. In this question, one mark is available for the quality of the use and organisation of
scientific terms.
Obesity is one of the risk factors that increases the chance of coronary heart disease
(CHD).
Explain how poor diet and obesity can affect the health of the heart.
[7]
[Total 8 marks]
149. The diagram below is a representation of the three-dimensional structure of an

antibody molecule. The shaded sections represent the heavy polypeptide chains. The
diagram shows two antigen molecules attached to the antibody.
antigen
A
antigen
A
Q
Anne White Biological Science Review 1993
(i)
Select the letter P, Q, R or S, which identifies the position of a variable region of

the antibody shown in the diagram above.
.........................................................................................................................
[1]
(ii)
Explain why this antibody will bind only to antigen A.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 3 marks]
150. MMR vaccine is a triple vaccine that contains antigenic material from measles, mumps
and rubella. It gives 90% of all children who are vaccinated protection against measles.
In the UK, the highest percentage of children in any year group that has been given the
MMR vaccine is 92%.
(i)
Calculate the percentage of children who were left unprotected against measles
in the year that a 92% vaccination rate was achieved. Show your working.
Answer = ..................................................%
[2]
(ii)
Measles has proved to be difficult to eradicate from any country and vaccination
programmes have been less successful than with smallpox.
Suggest two reasons why measles has been more difficult to eradicate than
smallpox.
1 ......................................................................................................................
.........................................................................................................................
2 ......................................................................................................................
.........................................................................................................................
[2]
[Total 4 marks]
151. Preserving the diversity of life on Earth has come to be an accepted goal for many
people.
However, this goal can sometimes come into conflict with other goals, such as
economic development.
In 1980, the International Union for the Conservation of Nature and Natural Resources
(IUCN) proposed a statement to form the basis for conserving biodiversity. One of the
points included in the statement is:
All species have an inherent right to exist. The ecological processes that support the
integrity of the biosphere and its diverse species and habitats are to be maintained.
Within the UK, many initiatives have been set up to help maintain biodiversity. One is
the Dartmoor Biodiversity Project, part of which is the Habitat Action Plan for moorland,
which covers almost 50% of the National Park.
This Action Plan specifies many objectives, all of which aim to maintain the range of
habitats on Dartmoor and ensure that all native plants and animals continue to breed
successfully and maintain healthy populations.
(a)
State what is meant by the term biodiversity.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(b)
grammar.
Outline the ecological, economic and ethical reasons behind initiatives such as
the Dartmoor Biodiversity Project and other similar projects around the world.
(Allow one line page)
[8]
(c)
State four activities of conservation organisations, such as the Royal Society for
the Protection of Birds, which contribute to the maintenance of biodiversity.
1 ......................................................................................................................
2 ......................................................................................................................
3 ......................................................................................................................
4 ......................................................................................................................
[4]
[Total 15 marks]
152. Scientists have discovered that certain microorganisms can survive in the Antarctic,
completely surrounded by ice.
Suggest how this discovery was useful in the development of certain biological washing
powders.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 2 marks]
153. In this question, one mark is available for the quality of written communication.
Some of the properties of water are listed below.
boils at 100 C
freezes at 0 C
water below 4 C is less dense than water above 4 C
excellent solvent
much energy is required to raise the temperature of water
much energy is required to change water into water vapour
high surface tension and cohesion
Describe and explain the roles of water in living organisms and as an environment for
living organisms.
You will gain credit for using information about the properties of water.
(Allow one and a half lined pages).
[9]
[Total 10 marks]
154. DNA and RNA are nucleic acids.

State two ways in which the structure of DNA differs from that of RNA.
1 ...............................................................................................................................
2 ...............................................................................................................................
[Total 2 marks]
155. The DNA molecule is made of two chains of nucleotides, wound into a double helix.
(i)
Describe the structure of a DNA nucleotide.

You may use the space below to draw a diagram if it will help your description.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(ii)
Describe how the two nucleotide chains in DNA are bonded together.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 6 marks]
156. An enzyme, such as amylase, has a specific 3-dimensional shape.

Explain how DNA structure determines the specific shape of enzymes.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 4 marks]
157. Read the following passage carefully, then answer the questions below.
10
15
Rhizobium is a bacterium that is closely associated with the roots of certain

plants known as legumes. These plants produce chemicals to attract the
bacteria and extra root hairs are produced. The bacteria attach to the surface
of the root hairs. Chemical links are formed between a complex
polysaccharide on the bacterial surface and lectin, a protein, formed by the
plants. The bacteria penetrate the cell walls of the root hairs and enter the
cells. The presence of the bacteria stimulates the cells of the root to divide,
forming swellings known as nodules.
The bacteria produce an enzyme, nitrogenase, that is the catalyst for the
conversion of nitrogen gas to ammonia. The bacteria use carbon compounds
manufactured by the plant to respire, making energy available for this
conversion. The ammonia is then used to form amino acids. Nitrogenase only
functions in low oxygen concentrations. The root cells produce a pigment,
leghaemoglobin, that is very similar to haemoglobin. Leghaemoglobin absorbs
oxygen, leaving low concentrations in the nodules.
(i)
Rhizobium is a prokaryotic organism.

State one characteristic that is typical of prokaryotes, but not of eukaryotes.
.........................................................................................................................
.........................................................................................................................
[1]
(ii)
Lectin (line 5) and polysaccharides are compounds that are formed from small
molecules joined together by chemical bonds.
Explain how the small molecules are joined together to form these compounds.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(iii)
Leghaemoglobin contains the same metal element as haemoglobin.

Name this metal element.
.........................................................................................................................
[1]
(iv)
State the names of two proteins, other than lectin, mentioned in the passage.
1 ......................................................................................................................
2 ......................................................................................................................
[2]
(v)
Name the process that occurs in Rhizobium to convert nitrogen gas into
ammonia.
.........................................................................................................................
[1]
(vi)
It has been suggested that oxygen is an inhibitor of nitrogenase.

Explain one way in which oxygen could act as an inhibitor.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 10 marks]
158. Fats in the diet provide the two essential fatty acids, linoleic acid and linolenic acid.
(a)
Give one reason why these two fatty acids must be present in the diet.
.........................................................................................................................
.........................................................................................................................
[1]
A 25 year old woman is concerned that she may be eating too much saturated fat.
She discovers that there are Dietary Reference Values (DRVs) for fats. These include:
total fat in the diet should not be greater than 35% of the total energy intake per day
of this no more than 10% should be saturated fat.
She calculates that her total energy intake should be 8 830 kJ per day.
Each gram of fat provides 37 kJ.
(b)
Calculate the maximum mass of fat in grams that the woman could consume if
she is not to exceed the DRV for total fat in the diet.
Show your working and express your answer to the nearest whole number.
Answer ........................... g
[2]
(c)
Explain the reasons for limiting the quantity of fat in the diet.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
The woman decided to find out whether it is healthier to eat meat or fish. She
compared the composition of mackerel (fish) and stewing steak (meat).
Some of the information that she found is summarised in the table below.
mass per 100g
nutrient
mackerel (fish)
stewing steak (meat)
protein/g
18.7
30.9
vitamin A/g
45.0
0.0
vitamin D/g
25.0
0.0
calcium/mg
11.0
15.0
iron/mg
0.8
3.0
Data from Food Tables and Labelling, pp.32 and 33; 56 and 57, by A. E. Bender and D. A. Bender.
Published by Oxford University Press, 1999 (ISBN 0 19 832815 X).
(d)
Explain, using the data in the table, the advantages and disadvantages for the
health of the young woman of including mackerel in her diet instead of stewing
steak.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[4]
[Total 11 marks]
159. Most children have antibodies to measles in their bloodstream at birth, giving them a
natural immunity. The concentration of these antibodies decreases quite quickly after
birth. Between the ages of about 6 to 12 months the concentration is low enough to
make children susceptible to measles.
(a)
(i)
State the term given to the type of natural immunity described above.
................................................................................................................
[1]
(ii)
State how antibodies to measles come to be present in children at birth.

................................................................................................................
................................................................................................................
[1]
A two year old child, who had not been vaccinated against measles, became infected
with the disease. The concentration of antibodies specific to measles was measured in
samples of blood taken at intervals of time for the following ten years. The results for
the first eight weeks of this study are shown in the graph below.
antibody concentration / arbitrary units
8
7
6
5
4
3
2
1
0
0
infection
4
time / weeks
(b)
State the name of the type of cell that produces antibodies.

.........................................................................................................................
[1]
(c)
Explain why there is a delay between the time of infection and the first
appearance of antibodies in the blood.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(d)
This child is unlikely to develop the symptoms of measles if exposed to the

pathogen again.
Explain why.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
(e)
Some children fail to respond to the measles vaccine when it is given too early,
for example at or before 6 months of age.
Suggest why this is the case.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[2]
[Total 10 marks]
160. People in many countries, especially in Africa and South-East Asia, are at high risk of
acquiring malaria.
Blood collected from a person known to have malaria was examined in an electron
microscope. The diagram below shows a drawing made from an electron micrograph of
a red blood cell infected by malarial parasites.
(a)
State the name of the organism that causes malaria.

.........................................................................................................................
[1]
(b)
Explain how an infected person is likely to have acquired malaria.

.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(c)
In this question, one mark is available for the quality of written communication.
Outline the problems that are associated with controlling the spread of malaria.
[8]
[Total 13 marks]
161. The table below shows the death rates from coronary heart disease (CHD) of men and
women between the ages of 35 and 74 for some European countries. It also shows the
prevalence of cigarette smoking among men and women of all ages in those countries.
The prevalence of smoking is the percentage of men and women who smoke
cigarettes every day.
deaths from CHD/
deaths per 100 000
prevalence of
smoking/%
non-Mediterranean countries
men
women
men
Latvia
321
107
54
24
Russian Federation
459
153
70
27
United Kingdom
121
40
37
35
Finland
134
45
32
24
Czech Republic
210
70
37
25
Hungary
243
81
46
34
Greece
200
29
64
40
Italy
116
39
33
19
Spain
91
30
36
31
France
85
21
37
27
women
Mediterranean countries
(a)
Suggest one reason why health authorities are especially concerned about the
death rates from CHD for people in the 35 to 74 age group.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[1]
(b)
Using only the information given in the table, explain whether or not the following
hypotheses are supported by the data.
You should quote data from the table in support of your answers.
(i)
Mediterranean countries have lower death rates from CHD than nonMediterranean countries.
supported or not supported ....................................................................
explanation .............................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
(ii)
Men are more at risk of CHD than women.

explanation .............................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
(iii)
Death rates from CHD are highest in countries with the highest prevalence
of smoking.
explanation .............................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[5]
[Total 6 marks]
162. A 50 year old man who regards himself as in good health was asked by an insurance
company to answer a series of questions about his health.
Here are some of his answers to the questions:
smoke 20 cigarettes a day
drink at least 8 pints of beer a week - most on Saturday evenings
play football in the park on Sundays
drive to work
spend most evenings at home watching television
rarely eat fresh fruit and vegetables
father died of a heart attack at age 45.
Use the answers above to explain the statement health is more than simply the
absence of disease.
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 3 marks]
163. State the word or phrase that best describes a region on the surface of an enzyme
molecule where a substrate can bind.
..................................................................................................................................
[Total 1 mark]
164. State the word or phrase that best describes the energy that must be provided for a
chemical reaction to take place.
..................................................................................................................................
[Total 1 mark]
165. State the word or phrase that best describes a length of DNA that codes for a particular
polypeptide.
..................................................................................................................................
[Total 1 mark]
166. A student was carrying out tests to determine which biological molecules were present
in a food sample.
(a)
(i)
Describe a test that the student could carry out to discover whether this
sample contained a lipid.
................................................................................................................
................................................................................................................
................................................................................................................
................................................................................................................
[2]
(ii)
State what would be seen if a lipid was present.

................................................................................................................
[1]
(b)
Describe how the structure of a phospholipid differs from that of a triglyceride.

You may use the space below for a diagram to help your answer.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(c)
(i)
Describe a test that the student could carry out to discover whether the
food sample contained protein.
................................................................................................................
................................................................................................................
................................................................................................................
[1]
(ii)
State what would be seen if protein was present.

................................................................................................................
[1]
[Total 8 marks]
167. Explain what is meant by the primary and secondary structure of a protein.
primary structure
..................................................................................................................................
..................................................................................................................................
secondary structure
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
..................................................................................................................................
[Total 5 marks]
168. An important enzyme that is used in respiration is succinate dehydrogenase. Its

substrate is succinate, which is converted into fumarate. Malonate acts as a
competitive inhibitor, but does not bind permanently to the enzyme.
(a)
Describe how malonate inhibits the enzyme. You may use the space below for a
diagram to help your answer.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(b)
Explain what would happen to the reaction if the concentration of succinate was
increased relative to the concentration of malonate.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 6 marks]
169. The following passage is taken from a medical encyclopaedia.

Smokers may develop two serious conditions. Firstly they may experience
breathlessness, wheezing and a cough which develops as sputum is produced. As the
smoker ages, the condition may become permanent and disable the smoker. This can
be associated with the second condition. Symptoms may include a barrel-shaped
chest, an oxygen deficiency that limits the possibility of exercise and a blueness of the
skin. If the condition advances, oxygen may need to be supplied through a mask. Name the two medical conditions described in the passage.
1 ................................................................................................................................
2 ...............................................................................................................................
[Total 2 marks]
170. (a)
The diagram below shows a cross section of a coronary artery from a patient who
had a heart attack.
Describe how changes in the walls of coronary arteries make it likely that a blood
clot will develop.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
(b)
Outline how nicotine and carbon monoxide in cigarette smoke may increase the
risks of atherosclerosis and blood clotting.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[3]
[Total 6 marks]
171. Fig. 1 is a diagram that shows the origin and maturation of lymphocytes.
Fig. 1
Fig. 2 shows the changes that occur to B and T lymphocytes during an infection by a
pathogen, such as a virus.
Fig. 2
Complete the following passage using the most appropriate terms.

The cells of the immune system originate from ..........................
...............................
where stem cells divide by mitosis to produce cells that differentiate into lymphocytes
and ..................................... .
Immature T lymphocytes migrate to the ................................. gland where they mature.
Mature B lymphocytes and mature T lymphocytes circulate and enter lymph nodes.
During an immune response some B lymphocytes differentiate into ..............................
................................. and secrete ................................. .
[Total 5 marks]
172. Fig. 1 is a diagram that shows the origin and maturation of lymphocytes.
Fig. 1
Fig. 2 shows the changes that occur to B and T lymphocytes during an infection by a
pathogen, such as a virus.
Fig. 2
In this question, one mark is available for the quality of written communication.
Describe the changes that occur to T lymphocytes during an immune response.
Explain the roles of T lymphocytes in fighting an infection by a pathogen, such as a
virus.
You may use information from Fig. 2 in your answer.
[7]
[Total 8 marks]
173. HIV is transmitted in a variety of ways.

In the UK, most of the HIV infections reported to the Communicable Disease
Surveillance Centre (CDSC) have occurred as a result of sex between men.
Describe three ways in which HIV is transmitted, other than during sexual activity.
1 ...............................................................................................................................
..................................................................................................................................
2 ...............................................................................................................................
..................................................................................................................................
3 ...............................................................................................................................
..................................................................................................................................
[Total 3 marks]
174. HIV is transmitted in a variety of ways.

In the UK, most of the HIV infections reported to the Communicable Disease
Surveillance Centre (CDSC) have occurred as a result of sex between men.
The CDSC collects statistics on HIV/AIDS and estimates the total number of people
who are infected with HIV. This increases in the UK each year and in 2002, the CDSC
estimated the number to be about 33 500. It also estimated that 30% of these people
were not diagnosed.
The graph below shows the numbers of new HIV and AIDS diagnoses and deaths in
those infected through sex between men in the UK between1985 and 2001.
2400
key
new HIV diagnoses
new AIDS diagnoses
deaths from AIDS
2000
1600
number
of men
1200
800
400
0
1985 1986 1987 1988 1989 1990 19911992 1993 1994 1995 1996 1997 1998 1999 2000 2001
year
(i)
Using the data in the graph, describe the changes that have occurred in the
numbers of men diagnosed with HIV between 1985 and 2001.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
(ii)
Explain the decrease in number of men who have been diagnosed with AIDS
and who have died from HIV/AIDS since 1994.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
(iii)
Using the data in the graph, explain why the number of people infected with HIV
in the male homosexual population in the UK is increasing.
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
.........................................................................................................................
[Total 6 marks]

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1.

A balanced diet is essential for good health.

Calculate the BMI of a female of mass 69 kg and a height of 1.67 m.

A way of determining whether a person is underweight or overweight is to use a graph

100 110 120 130 140 150

Name two diseases associated with obesity.

The figure below represents a water molecule.

Water molecules are polar. As a result, they attract each other.

the bond(s) between the two molecules

Ponds provide a very stable environment for aquatic organisms.

Water is important in many biological reactions.

the type of reaction that occurs

The enzyme DHPS is involved in the production of folic acid in bacteria.

The substrate for DHPS is a molecule known as PABA.

The enzyme DHPS is inhibited by the drug sulfonamide.

Antibiotic resistance in bacteria is becoming an increasing problem.

Hospitals can check to see if a strain of bacteria causing an infection is resistant to a

The bacteria are isolated from a patient.

Complete the table by selecting the letter, J, K, L or M, that represents the

The primary structure of a protein consists of a chain of amino acids.

Each amino acid has a different R group.

Collagen is found in the ligaments which hold bones together at joints.

Coronary heart disease (CHD) can be described as a multifactorial disease. This

CHD due to high

CHD due to high

Smoking is a contributing factor in 19% of all cases of CHD.

increases heart rate

Cholesterol is transported in the form of lipoproteins. High levels of low density

DNA and RNA are nucleic acids.

State the components of a DNA nucleotide.

Describe how the structure of RNA differs from that of DNA.

Before a cell divides, the DNA needs to be accurately replicated.

State what a gene codes for.

Define the terms:

percentage cover of each plant species

The format of the students table is incorrect.

Describe the distribution of bracken.

The student calculated Simpsons Index as 0.2. This is a low value.

DNA is found in the nucleus. The molecule is twisted into a ..................................

relative proportions of bases in DNA

DNA in the nucleus acts as a template for the production of RNA.

DNA codes for the structure of polypeptides.

Name the parasite that causes malaria.

Name the vector for the malarial parasite.

Name a human cell in which the malarial parasite reproduces.

Scientists are developing a vaccine using an attenuated (inactive) form of the

Name bonds A and B shown in the figure.

State how bond B is broken in the digestion of the disaccharide.

rate of enzyme activity (arbitrary units)

Explain why the activity of enzyme 2 falls to zero at pH 7.

vultures feed on carcasses including those from farm animals.

Suggest what is meant by critically endangered.

Calculate the original population of the white-backed vulture.

vultures feed on carcasses including those from farm animals.

collected from several different areas

housed in three separate centres.

vultures feed on carcasses including those from farm animals.

vultures feed on carcasses including those from farm animals.

Name two kingdoms other than Prokaryotae, Fungi and Plantae.