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    Animal Care and Use Statement

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    Von Claude Galinato Alingcayon
    This study aims to investigate the effects of quail eggs in increasing platelet levels in rats with induced thrombocytopenia. Specifically, it will determine if quail eggs are effective in increasing platelet counts and if higher consumption leads to higher platelet values. The study will be conducted from February to March 2015 involving 15 rats divided into groups. One group will be the standard, one the negative control, and one will consume quail eggs after inducing thrombocytopenia to study the treatment effect. Spleen dissection and smear analysis will be performed to analyze platelet levels.

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    Animal Care and Use Statement

    Uploaded by

    Von Claude Galinato Alingcayon
    234 views3 pages
    This study aims to investigate the effects of quail eggs in increasing platelet levels in rats with induced thrombocytopenia. Specifically, it will determine if quail eggs are effective in increasing platelet counts and if higher consumption leads to higher platelet values. The study will be conducted from February to March 2015 involving 15 rats divided into groups. One group will be the standard, one the negative control, and one will consume quail eggs after inducing thrombocytopenia to study the treatment effect. Spleen dissection and smear analysis will be performed to analyze platelet levels.

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    This study aims to investigate the effects of quail eggs in increasing platelet levels in rats with induced thrombocytopenia. Specifically, it will determine if quail eggs are effective in increasing platelet counts and if higher consumption leads to higher platelet values. The study will be conducted from February to March 2015 involving 15 rats divided into groups. One group will be the standard, one the negative control, and one will consume quail eggs after inducing thrombocytopenia to study the treatment effect. Spleen dissection and smear analysis will be performed to analyze platelet levels.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    234 views3 pages

    Animal Care and Use Statement

    Uploaded by

    Von Claude Galinato Alingcayon
    This study aims to investigate the effects of quail eggs in increasing platelet levels in rats with induced thrombocytopenia. Specifically, it will determine if quail eggs are effective in increasing platelet counts and if higher consumption leads to higher platelet values. The study will be conducted from February to March 2015 involving 15 rats divided into groups. One group will be the standard, one the negative control, and one will consume quail eggs after inducing thrombocytopenia to study the treatment effect. Spleen dissection and smear analysis will be performed to analyze platelet levels.

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    ANIMAL CARE AND USE STATEMENT

    (Protocol and Review Form)


    I.

    PROCEDURE(S) OR TITLE OF RESEARCH STUDY:


    Pro-megakaryopoietic
    Thrombocytopenia

    II.

    effects

    of

    Quail

    Eggs

    in

    Rats

    with

    Induced

    PURPOSE/ OBJECTIVES:
    This study aims to investigate the pro-megakaryopoietic effect of quail eggs in
    rats with induced thrombocytopenia. Specifically to determine:
    a. The effectiveness of quail eggs in increasing platelet levels.
    b. Whether greater consumption of quail eggs leads to higher platelet values.

    III.

    DURATION OR TIME FRAME:


    The research study will be conducted on February up to March, 2015.

    IV.

    RESPONSIBLE PERSON OR PRINCIPAL INVESTIGATOR:


    a. NAME: MARJI SIM JAMIAS-RAGANIT
    b. QUALIFICATION (degree or qualification/s): REGISTERED MEDICAL
    TECHNOLOGIST, MSMT

    V.

    BACKGROUND AND SIGNIFICANCE OF THE PROCEDURE OR RESEARCH:


    This study aims to investigate the pro-megakaryopoietic effect of quail eggs in
    rats with induced thrombocytopenia.

    VI.

    DESCRIPTION OF METHODOLOGIES/ EXPERIMENTAL DESIGN:


    Quail eggs will be administered to Group C rats with induced
    thrombocytopenia through oral gavage. The rats to be used are female albino
    weighing approximately 100 to 150 grams. Fifteen rats will be needed in the research
    study. The rats will be divided into three groups consisting five rats each and will be
    placed in a cage. Group A will be the standard, group B will be the negative control,
    and group C will be the treatment group. Food and drink will be given to the rats as
    diet for the one week acclimatization at the SLU Animal House. Hydroxyurea will be
    orally administered to Group B and group C to induce thrombocytopenia.
    Administration of quail eggs to group C only will be done for fourteen days.
    Dissection and smear preparation of spleen of all the rats will be performed by a
    licensed veterinarian at Benguet State University. A pathologist will be reading the
    results of the smears at Saint Louis University Laboratory.

    VII.

    DECLARATION BY THE RESPONSIBLE PERSON:


    I ACCEPT RESPONSIBILITY FOR ASSURING THAT THE PROCEDURE/
    STUDY WILL BE IN ACCORDANCE WITH THE APPROVED PROTOCOL. I
    ASSURE THAT ALL PERSON WHO USE THIS PROTOCOL AND WORK
    WITH ANIMALS HAS RECEIVED APPROPRIATE
    TRAINING/INSTRUCTIONS IN PROCEDURAL AND HANDLING
    TECHNIQUES, AND ON ANIMAL WELFARE CONSIDERATIONS.
    I AGREE TO OBTAIN WRITTEN APPROVAL FROM THE INSTITUTIONAL
    ANIMAL CARE AND USE COMMITTEE PRIOR TO MAKING ANY
    CHANGES AFFECTING MY PROTOCOL. I ALSO AGREE PROMPTLY
    NOTIFY THE IACUC IN WRITING OF ANY EMERGENT PROBLEMS THAT
    MAY ARISE IN THE COURSE OF THIS STUDY INCLUDING THE
    OCCURRENCE OF ADVERSE SIDE EFFECTS.

    Signature of the Responsible Person:

    _________________________

    Date:___________________

    Noted:

    RUTH C. DIEGO
    Chair, BSU-IACUC

    Date:___________________

    APPLICATION FOR AUTHORIZATION


    (For the Conduct of Scientific Procedures using Animals)

    1. Name of Entity: Pro-megakaryopoietic effects of Quail Eggs in Rats with Induced


    Thrombocytopenia
    2. Address: Saint Louis University, Baguio City
    3. Telephone number: 444-8246
    4. Name and Position of Representative Person:
    RAGANIT
    Last Name

    MARJI SIM
    First Name

    JAMIAS
    Middle Name

    Position: RESEARCH PROMOTER


    5. Description/ Profile of entity (attach organization chart):
    This study is A Research Proposal in Partial Fulfillment of the Requirements for the
    Degree, Bachelor in Medical Laboratory Science.
    6. Purpose of the conduct of Scientific Procedures (encircle one or more):
    a. Biochemical research experiment, studies, investigation (including pre-clinical
    research)
    b. Teaching and Instruction
    c. Product testing
    d. Production of antiserum or other biological
    7. Identify the key Instructional representatives (including the ACLUC Chairperson,
    Veterinarians, and researcher)
    I certify that the statements made herein are correct and true:

    ______________________

    __________________________

    Signature of Representative

    Signature of Head of Institutions

    Date:__________________

    Date: _____________________

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