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    Activity 2a - Qualitative Test For Proteins

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    Dave Marimon
    The document describes qualitative tests performed to detect proteins in a sample. Six different color reaction tests were conducted - Millon's test, Xanthoproteic test, Glyoxylic acid test, Biuret test, Ninhydrin test, and Sakaguchi test for arginine. The results of each test are recorded, with four tests being negative and two positive for the presence of proteins. An analysis section then explains the principle behind each test for proteins.

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    Activity 2a - Qualitative Test For Proteins

    Uploaded by

    Dave Marimon
    485 views4 pages
    The document describes qualitative tests performed to detect proteins in a sample. Six different color reaction tests were conducted - Millon's test, Xanthoproteic test, Glyoxylic acid test, Biuret test, Ninhydrin test, and Sakaguchi test for arginine. The results of each test are recorded, with four tests being negative and two positive for the presence of proteins. An analysis section then explains the principle behind each test for proteins.

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    Color reaction tests

    Original Title

    ACTIVITY 2A - QUALITATIVE TEST FOR PROTEINS

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    The document describes qualitative tests performed to detect proteins in a sample. Six different color reaction tests were conducted - Millon's test, Xanthoproteic test, Glyoxylic acid test, Biuret test, Ninhydrin test, and Sakaguchi test for arginine. The results of each test are recorded, with four tests being negative and two positive for the presence of proteins. An analysis section then explains the principle behind each test for proteins.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    485 views4 pages

    Activity 2a - Qualitative Test For Proteins

    Uploaded by

    Dave Marimon
    The document describes qualitative tests performed to detect proteins in a sample. Six different color reaction tests were conducted - Millon's test, Xanthoproteic test, Glyoxylic acid test, Biuret test, Ninhydrin test, and Sakaguchi test for arginine. The results of each test are recorded, with four tests being negative and two positive for the presence of proteins. An analysis section then explains the principle behind each test for proteins.

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    ACITIVITY 2A

    QUALITATIVE TEST FOR PROTEINS/COLOR REACTION TEST


    GROUP 6
    MARIMON, Dave Michael C.
    MACION, Ayn Rand Louise V.
    TANDUYAN, Maria Cristalia
    RUIZO, Roichinne
    DATUMANONG, Madatu Datudick Jr.
    BAID, Ivy Zea
    MALIRONG, Chiezyl

    OBJECTIVES:

    To detect proteins through the different color reaction tests.


    To know the significance of peptide bonds and amino acids in detecting proteins.
    To understand the principle behind every test.

    BMLS 2H
    April 21, 2014

    RESULTS AND OBSERVATION:


    NAME OF TEST

    SUBSTANCE TO
    BE TESTED

    REAGENTS USED

    VOLUME OF
    REAGENTS USED

    OBSERVATIONS/RESULTS

    Millons Test

    Proteins

    Millons Reagent

    2 drops

    The solution DOES NOT have flesh precipitate to red color. NEGATIVE
    (-)

    Xanthoproteic Test

    Proteins

    Concentrated Nitric
    Acid

    5 drops

    The solution is cloudy color yellow with residue remained.


    POSITIVE ( + )

    5 drops
    Ammonium Hydroxide
    Glyoxylic Acid
    Reactions
    (Hopkins Cole)

    Proteins

    Hopkins Cole
    Reagent

    1 mL
    10 drops

    The solution DOES NOT have a violet ring that will form between the 2
    liquids. NEGATIVE ( - )

    Pure Concentrated
    Sulfuric Acid
    Biuret Test

    Proteins

    10% Sodium
    Hydroxide

    1 mL

    The solution is clear blue and violet.


    POSITIVE ( + )

    1 mL
    0.5% Copper Sulfate
    Ninhydrin Test

    Proteins

    Sakaguchi Reaction
    for Arginine

    Proteins

    0.1% aqueous solution


    of Ninhydrin
    10% Sodium
    Hydroxide

    1 mL

    The solution DID NOT produce a blue color. NEGATIVE ( - )

    1 mL
    6 drops

    Diltute Alcoholic
    a-naphthol solution

    The solution DID NOT produce a red color. NEGATIVE ( - )


    10 drops

    Sodium Hypobromite
    Solution

    PROOF/DOCUMENTATION:

    FROM LEFT TO RIGHT:


    A. MILLONS TEST
    B. XANTHOPROTEIC TEST
    C. GLYOXYLIC ACID REACTIONS (HOPKINS COLE)
    D. BIURET TEST
    E. NINHYDRIN TEST
    F. SAKAGUCHI REACTION FOR ARGININE

    ANALYSIS AND CONCLUSION: GUIDE QUESTIONS


    1. Explain the principle involved in each of the test for proteins.

    Millons test
    Millons reagent gives the positive results with proteins containing the phenolic amino acid tyrosine, the precipitate turns flesh red
    color, proteins containing tyrosine are present.

    Xanthoproteic test
    - Determined the amount of the protein soluble in a solution using the concentrated Nitric acid. The test gives the positive result in those
    proteins with amino acid carrying aromatic groups especially tyrosine. The proof is neutralized with an alkali, turning dark yellow.

    Hopkins Cole test


    There was a violet color produced at the point of contact. Tryptophan has an in dole nucleus which is responsible for the violet ring
    found at the junction between the two layers.

    Biuret test

    Ninhydrin test
    - Amino acids containing a free amino group and a free carboxylic acid group that react together with ninhydrin to produce color
    products. Group on the alpha- carbon and can react with ninhydrin to produce blue purple product.

    Sakaguchi test

    Reduction of Cu2+ which then complexes with the N atoms on the peptide bonds at high Ph.

    - In alkaline solution, protein containing Arginine gives red color with Alpha-Napthol and Sodium hypochloride.

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