Scribd Logo
Upload

Welcome to Scribd!

  • 83 views

    Chapter One

    Uploaded by

    Arjun Kataria
    The document summarizes key points about pH and buffers from a biochemistry practical group assignment. It includes: 1) Definitions of pH as the negative logarithm of hydrogen ion concentration and how pH is measured using indicators and meters. 2) An explanation of what buffers are and how they resist changes in pH. Important buffer systems in the human body are discussed like bicarbonate buffers in blood. 3) The Henderson-Hasselbalch equation is presented as a way to calculate pH from concentrations of acid/base and pKa.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Chapter One

    Uploaded by

    Arjun Kataria
    83 views6 pages
    The document summarizes key points about pH and buffers from a biochemistry practical group assignment. It includes: 1) Definitions of pH as the negative logarithm of hydrogen ion concentration and how pH is measured using indicators and meters. 2) An explanation of what buffers are and how they resist changes in pH. Important buffer systems in the human body are discussed like bicarbonate buffers in blood. 3) The Henderson-Hasselbalch equation is presented as a way to calculate pH from concentrations of acid/base and pKa.

    Original Description:

    Chapter one

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    The document summarizes key points about pH and buffers from a biochemistry practical group assignment. It includes: 1) Definitions of pH as the negative logarithm of hydrogen ion concentration and how pH is measured using indicators and meters. 2) An explanation of what buffers are and how they resist changes in pH. Important buffer systems in the human body are discussed like bicarbonate buffers in blood. 3) The Henderson-Hasselbalch equation is presented as a way to calculate pH from concentrations of acid/base and pKa.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    83 views6 pages

    Chapter One

    Uploaded by

    Arjun Kataria
    The document summarizes key points about pH and buffers from a biochemistry practical group assignment. It includes: 1) Definitions of pH as the negative logarithm of hydrogen ion concentration and how pH is measured using indicators and meters. 2) An explanation of what buffers are and how they resist changes in pH. Important buffer systems in the human body are discussed like bicarbonate buffers in blood. 3) The Henderson-Hasselbalch equation is presented as a way to calculate pH from concentrations of acid/base and pKa.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    You are on page 1of 6

    Group-2 biochemistry practical:

    Subject : biochemistry

    Topic : pH & buffers

    Lecturer: Alexander N. Tan Jr., M.D.

    Group members :

    1. Arjun kataria
    2. Parth faldu
    3. Nayan gadhiya
    4. Roshani chaudhari
    5. Pallak gautam
    6. Krishna godhani
    7. Sophia givertz
    8. Anurag kamlakar
    9. Sandesh gamot
    CHAPTER ONE: pH AND BUFFERS

    pH :

    the pH of solution is the common logarithm of the reciprocal of the


    hydrogen ion concentration expressed as:

    ph=log 1/H+

    pH is detected and measured by using a pH meter, but the most common


    detectors are the color changes of acid-base indicators. an acid-base
    indicator is a weak acid. the ionized and unionized forms of the indicator
    have different colors:

    Hn + H2O <====> H+ + in-

    When the indicator is placed in an solution the equilibrium is shifted


    to the Hin form predominants. In an alkaline solution depends upon the
    ratio of Hn and is related to pH in the following equaton:

    Log10 [in-]/ [HIn] = pH-pKa

    (comes from Handerson-Hasselbalch equation)

    The color of the indicator solution is therefore a measure of the pH.

    BUFFERS :

    A buffer solution is one of that resits a change solution in pH when a


    small amount of acid or base is added. A buffer solution contain a weak
    acid, HA and its conjugate base. An explain is the mixture of acetic acid
    and sodium acetate.
    Buffers are significant in biochemistry. An excellent example is its in
    blood. blood contains phosphates, carbohydrates, and proteins as buffering
    components and has a content pH between 7.35 and 7.45 even though
    there are large amount of acids and bases contantly introduced by
    designation of foods, absorption, muscular activity and respiration.

    Procedure:

    pH determination

    1. prepare the following samples:

    a. defibrinated blood

    b. fresh milk

    c. freshly volded urine

    2. Determine the approximate pH of the sample using the pH paper by


    following this steps:

    a. dip a piece of paper in the sample for about 10 seconds. remove the
    paper and place it on a watch glass.

    b.match the color produced in the pH paper with the color chart to
    determine the pH of the sample.

    c.record this results.

    3.determine the accurate pH of the samples using the pH meter.


    QUESTIONS AND PROBLEMS:

    1. What is the H+ concentration of a phosphate buffer solution if its


    pH is 7.4?
    Ans.

    pH=7.4
    [H+]=?
    Now pH = log1/[H+]
    7.4 = log1/[H+]
    [H+] = log1/
    [H+] = 3.98 X 10-8 M
    So like this we can find H+ concentration of any solution by
    using this equation

    2. What is the pH of a 0.125 M NaOH solution? (clue: pH + pOH =


    14)
    Ans.

    Same as above with some twist …..

    pH= ??
    [OH-]=0.125 M

    So pOH = -log[OH-]
    pOH = invlog

    3. What is the significant of buffer systems in humans?


    Ans. There are three buffer systems in the human body. The protein
    buffer system is the most important and widely operating buffer in the
    bodies fluid. An example of a protein buffer is hemoglobin. The
    phosphate buffer system is made up of two ions, hydrogen phosphate
    ion, and dihydrogen phosphate ion. The bicarbonate buffer system
    function is to maintain the pH level in blood mammals. The
    bicarbonate buffer system neutralize the pH enzymes in the stomach
    and intestines.

    4. What is the Henderson-hasselbalch equation?explain.


    Ans.

    In chemistry, the Henderson–Hasselbach equation describes the
    derivation of pH as a measure of acidity (using pKa, the negative log
    of the acid dissociation constant) in biological and chemical systems.
    The equation is also useful for estimating the pH of a buffer solution
    and finding the equilibrium pH in acid-base reactions (it is widely used
    to calculate the isoelectric point of proteins).
    The equation is given by:

    pH = pKa + log [A-]/[HA]

    5. what is the normal pH of blood?urine?gastric juice? why is it


    necessary to know the pH of these physiologic fluids?

    Ans. Normal ph of the …


    lood ph

    Urine ph

    Gastric juice ph–


    6.what is the biochemical relevance of pH?

    Ans. pH is a measure of acidity or alkalinity and is measured on a scale of


    0-14, with 0 being 100% acidic and 14 being 100% basic. A pH of 7 is
    neutral, neither acidic or basic. pH has relevance to biochemical systems,
    because most biological systems require a mainly neutral pH environment
    to survive. For example, the pH of blood is 7.4 with a very narrow range.

    7.calculate the ph of 0.01 M HCL

    You might also like