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    Mitotic Pool: 1' Granules: Large Blue To Reddish-Purple Staining

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    Dan Paolo Sanchez
    There are 3 pools of bone marrow that produce white blood cells: the mitotic pool, maturation pool, and storage pool. Cells progress through different stages in each pool, starting as myeloblasts in the mitotic pool and maturing into segmented neutrophils, eosinophils, or basophils in the storage pool. A similar process occurs in the lymphocyte and monocyte series. Mature cells then circulate or marginate in the blood.

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    Mitotic Pool: 1' Granules: Large Blue To Reddish-Purple Staining

    Uploaded by

    Dan Paolo Sanchez
    66 views6 pages
    There are 3 pools of bone marrow that produce white blood cells: the mitotic pool, maturation pool, and storage pool. Cells progress through different stages in each pool, starting as myeloblasts in the mitotic pool and maturing into segmented neutrophils, eosinophils, or basophils in the storage pool. A similar process occurs in the lymphocyte and monocyte series. Mature cells then circulate or marginate in the blood.

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    Gran Ulo Poiesis

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    There are 3 pools of bone marrow that produce white blood cells: the mitotic pool, maturation pool, and storage pool. Cells progress through different stages in each pool, starting as myeloblasts in the mitotic pool and maturing into segmented neutrophils, eosinophils, or basophils in the storage pool. A similar process occurs in the lymphocyte and monocyte series. Mature cells then circulate or marginate in the blood.

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    Download as docx, pdf, or txt
    66 views6 pages

    Mitotic Pool: 1' Granules: Large Blue To Reddish-Purple Staining

    Uploaded by

    Dan Paolo Sanchez
    There are 3 pools of bone marrow that produce white blood cells: the mitotic pool, maturation pool, and storage pool. Cells progress through different stages in each pool, starting as myeloblasts in the mitotic pool and maturing into segmented neutrophils, eosinophils, or basophils in the storage pool. A similar process occurs in the lymphocyte and monocyte series. Mature cells then circulate or marginate in the blood.

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    GRANULOPOIESIS

    3 pools of bone marrow 1. mitotic pool


    2.maturation pool
    3.storage pool
    Mitotic pool Myeloblast
    Promyeloblast
    Myelocyte
    Maturation pool Metamyelocyte
    Storage pool Metamyelocyte
    Bands
    Segmented cells
    Segmented cells 1.neutrophils
    2.eosinophils
    3.basophils
    Pools of blood 1.circulating pool
    2.marginating pool
    marginating pool Not included in total WBC count and differential count

    Myeloblast Size: 15-20 u


    Nucleus: round or slightly oval; fine chromatin
    Nucleoli: 2-5
    Cytoplasm: basophilic
    N:C ration: 4:1

    Promyelocyte Size: 15 – 21 u
    Nucleus: round or oval; slightly coarse chromatin
    Nucleoli: 2-3
    Cytoplasm: with primary granules / non – specific granules
    N:C ration: 3:1 – 2:1

    1’ granules: large blue to reddish- purple staining

    Acid hydrolase,
    acid phosphatase,
    glucoronidase,
    myeloperoxidase,
    elastase,
    arylsulfatase,
    cationic antibacterial proteins,
    esterase,
    sulfated mucosubstance,
    arylsulfatase
    Myelocyte Size: 12 – 18 u
    Nucleus: round or oval; coarser chromatin
    Nucleoli: none
    Cytoplasm: slightly acidophilic; with secondary granules/ specific granules
    N:C ratio: 1:1
    Metamyelocyte /juvenile Size: 10 – 15 u
    Nucleus: kidney – shaped or indented; clumped chromatin
    Nucleoli: none
    Cytoplasm: salmon- colored; with secondary granules /specific granules
    N:C ratio: 1:1

    Band / stab /staff Size: 9- 15 u


    Nucleus: C or S -shaped; clumped chromatin
    Nucleoli: none
    Cytoplasm: salmon- colored; faint granules
    N:C ratio: 1:1 or 1:2

    Neutrophils Size: 9 – 15 u
    Nucleus: 3 -5 lobes; chromatin highly condensed
    Nucleoli: none
    Cytoplasm: salmon- colored; pink/ lavender / lilac / rose -violet granules
    N:C ratio: 1:2

    2’ granules: pink to rose- violet staining


    aminopeptidase,
    collagenase,
    muramidase,
    lysozyme,
    lactoferrin,
    basic proteins,
    plasminogen activator,
    alkaline phosphate

    3’ granules: gelatinase

    Eosinophils Size: 9-15 u


    Nucleus: 2 lobes; chromatin condensed
    Nucleoli: none
    Cytoplasm: salmon- colored; reddish- orange granules
    N:C ratio: 1:2

    2’ granules: dirty orange staining


    Major Basic Protein,
    acid hydrolase,
    peroxidase,
    phospholipase,
    cathepsin,
    eosinophil- cationic protein,
    neurotoxin

    arylsulfatase,
    peroxidase,
    acid phosphatase
    Basophils Size: 10 -16 u
    Nucleus: unsegmented or bilobed; chromatin condensed
    Nucleoli: none
    Cytoplasm: salmon- colored; dark- purple or blue-black
    N:C ratio: 1:2

    2’ granules: dark- blue purple staining


    histamine,
    heparin,
    peroxidase,
    eosinophilic chemotactic factor A

    LYMPHOCYTE SERIES

    LYMPHOCYTE
    LYMPHOBLAS PROLYMPHOC Small
    T YTE Medium
    Large
    Lymphoblast Size: 10- 18 u
    Nucleus: round or oval; coarse chromatin
    Nucleoli: 1 -2
    Cytoplasm: moderate to dark blue; no granules

    Prolymphocyte Size: 10 – 18 u
    Nucleus: round or oval; clumped chromatin
    Nucleoli: 1- 2
    Cytoplasm: moderate to dark blue; no granules

    Small lymphocyte Size: 7- 10 u


    Nucleus: round or oval; dense chromatin
    Nucleoli: none
    Cytoplasm: thin rim around the nucleus; ROBIN EGG BLUE

    Medium lymphocyte Size: 10 – 12 u


    Nucleus: round or oval; dense chromatin
    Nucleoli: none
    Cytoplasm: more abundant than in small lymphocyte; pale to moderate blue

    Large lymphocyte Size: 12 – 16 u


    Nucleus: round or oval; dense chromatin
    Nucleoli: none
    Cytoplasm: abundant; clear , very pale blue

    LYMPHOCYTES:
    1. T- lymphocytes: 60- 80 %; lifespan- 4- 10 years; cellular- mediated immunity
    2. B- lymphocytes: 10- 20 %; lifespan- 3-4 days; humoral- mediated immunity
    3. Null- lymphocytes: 10 %; tumor host defense
    MATURE B-
    ACTIVATED B- CELL PLASMA CELL
    CELL

    PLASMA CELL SERIES


    Plasma blast Size: 18 – 25u
    Nucleus: eccentric
    Cytoplasm: abundant; basophilic; no granules

    Proplasmacyte Size: 15 – 25 u
    Nucles: eccentric
    Cytoplasm: abundant; intensely basophilic; no granules

    Plasmacyte Size: 8- 20u


    Nucleus: eccentric; exhibits ‘cartwheel-like pattern’
    Cytoplasm: deeply basophilic; no granules; well- defined hof/ perinuclear halo

    MONOCYTE SERIES

    PROMONOCY
    MONOBLAST MONOCYTE
    TE
    Monoblast Size: 12- 20 u
    Nucleus: round, folding/ clefting
    Nucleoli: 1-2
    Cytoplasm: basophilic; non- granular
    N:C ratio: 4: 1- 3: 1

    Promonocyte Size: 14- 18 u


    Nucleus: oval, indented, or folded
    Nucleoli: none
    Cytoplasm: blue- gray
    N:C ration: 3: 1- 2: 1

    Monocyte Size: 14- 20 u


    Nucleus: round; kidney- shaped; convoluted
    Nucleoli: none
    Cytoplasm: blue- gray; fine azurophilic granules
    (ground glass/ frosted appearance)

    N:C ratio: 2: 1- 1: 1

    Tissue: macrophages/ histiocytes

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