Supercritical Fluid Extraction of Polyphenols From Grape Skin Using Carbon Dioxide and Ethanol As A Cosolvent: Kinetic Study and Extract Composition

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Supercritical fluid extraction of polyphenols from

grape skin using carbon dioxide and ethanol as a


cosolvent: kinetic study and extract composition
Fernando Villanuevaa, Jennifer Mezaa, Fredy Huaytaa, Fiorella Cardenas-Toroa,*
a
Department of Engineering, Section of Industrial Engineering, Faculty of Science and
Engineering. Pontifical Catholic University of Peru. Lima - Perú
* fcardenas@pucp.pe

In recent years, there is a growing interest of companies to develop new processes using
industrial residues and, mitigate the impact of their production activities on
environment. Furthermore, governments invest on research projects for the development
of clean technologies as an alternative to traditional techniques. One of these
technologies is the supercritical extraction technology, which uses solvents such as CO2,
ethanol and water (GRAS solvents) for the separation of high added value bioproducts.
In Peru, one of the main industries in the food and beverage sector is the wine industry,
which produces around 135000 tonnes annually of a residue composed mainly of 60%
of grape skin. Several studies showed that grape skin is a source of polyphenols with
beneficial health effects against chronic diseases such as coronary heart, and some
cancers. In the present work, a study of the supercritical extraction of polyphenols from
Malbec grape skin was performed. The experiments were carried out in a commercial
equipment with an extractor capacity of 0.1 L. The operating conditions were
temperature of 40, 50, 60 °C, pressure of 200, 300 and 400 bar, and 8.5% of ethanol
mass at a final flow rate of 0.14 m3/h. The kinetics of the extract yield, and the quality
of the extract in terms of total polyphenols were evaluated. The results indicated that
extract yield increased with pressure at temperature of 50 and 60°C, with highest value
at 400 bar and 60°C (7.4 g extract/100 g raw material dry basis), while polyphenol
content in extract varied between 1.7 – 3.0 mg GAE (gallic acid equivalent)/g extract,
with high values al 40°C. The polyphenol profile was also evaluated at selected
conditions.

Keywords: grape skin, polyphenols, supercritical extraction, carbon dioxide, ethanol

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