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    Jorgensen Laboratories, Inc.: Urine Sediment Stain

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    Loralie Paña
    This document provides information about the JorVet urine sediment stain, including its formulation and directions for use. The stain is a modification of the Sternheimer-Malbin urine stain that allows for easier recognition of leukocytes and "glitter cells" under microscopic examination. An included chart describes the expected colors that various elements in a urine sediment will take on when stained, to aid in microscopic interpretation and diagnosis.

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    Jorgensen Laboratories, Inc.: Urine Sediment Stain

    Uploaded by

    Loralie Paña
    115 views2 pages
    This document provides information about the JorVet urine sediment stain, including its formulation and directions for use. The stain is a modification of the Sternheimer-Malbin urine stain that allows for easier recognition of leukocytes and "glitter cells" under microscopic examination. An included chart describes the expected colors that various elements in a urine sediment will take on when stained, to aid in microscopic interpretation and diagnosis.

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    Urine Sediment Stain

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    This document provides information about the JorVet urine sediment stain, including its formulation and directions for use. The stain is a modification of the Sternheimer-Malbin urine stain that allows for easier recognition of leukocytes and "glitter cells" under microscopic examination. An included chart describes the expected colors that various elements in a urine sediment will take on when stained, to aid in microscopic interpretation and diagnosis.

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    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    115 views2 pages

    Jorgensen Laboratories, Inc.: Urine Sediment Stain

    Uploaded by

    Loralie Paña
    This document provides information about the JorVet urine sediment stain, including its formulation and directions for use. The stain is a modification of the Sternheimer-Malbin urine stain that allows for easier recognition of leukocytes and "glitter cells" under microscopic examination. An included chart describes the expected colors that various elements in a urine sediment will take on when stained, to aid in microscopic interpretation and diagnosis.

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    Jorgensen Laboratories, Inc.

    Loveland, CO 80538

    J-326U
    Urine Sediment Stain

    Microscopic examination of urine sediment is a valuable diagnostic tool in the


    evaluation of urinary tract disease.

    Due to the special refractile and varied nature of the formed substances in
    urine sediment, a specialized stain is required. The JorVet urine sediment
    stain is a stabilized modification of the Sternheimer-Malbin urine stain. This
    modification allows easier recognition of leukocytes and so-called glitter cells
    (another form of leukocyte).

    Directions for Use

    1. The urine specimen should be a freshly voided sample collected in a


    clean and sealed container. Refrigerated specimens can also be used. A
    non-refrigerated delay of longer than 4 hours can result in new microbial
    growth.

    2. Put in centrifuge tube and centrifuge for 5 minutes at about 1500 RPM.

    3. Remove the supernatant without disturbing the sediment.

    4. Add 1-2 drops of stain to the sediment in the tube.

    5. Flick the bottom of the tube several times sharply with a finger.

    6. Transfer one drop to a microslide. A cover slip should be used.

    7. Low power: will see casts and various crystals.


    High power: not presence of RBC and WBC’s per field.

    Interpretations
    See chart on reverse side

    Precautions
    - For invitro diagnostic use only
    - Harmful if swallowed
    - Store at room temperature

    Formulation
    Crystal Violet 0.10% Ethyl Alcohol 10.00%
    Safranin 0.25% Water & Stabilizers 89.62%
    Ammonium Oxalate 0.03%

    Catalog #J-326U
    Contents: 15 ml

    Made in USA • Jorgensen Laboratories, Inc. • Loveland, CO 80538


    ELEMENTS IN UNUSUAL DISTINGUISHING COLOR COMMENTS
    URINARY SEDIMENT OF STAINED ELEMENTS

    Red Blood Cells Neutral - pink to purple


    Acid - pink (unstained)
    Alkaline - purple

    Nucleii Cytoplasm

    White Blood Cells


    Dark Staining Cells purple purple
    granules

    Glitter Cells colorless or pale blue


    (Sternheimer Malbin light blue or grey
    positive cells)

    Renal Tubular dark shade of light shade


    Epithelial Cells blue-purple of blue-purple

    Bladder Tubular blue-purple light purple


    Epithelial Cells

    Squamous Epithelial Cells dark shade of light purple


    orange-purple or blue

    INCLUSIONS & MATRIX

    Hyaline Casts pale pink or pale purple Very uniform color.


    Slightly darker than
    mucous threads.

    Coarse Granular dark purple granules in purple


    Inclusion Casts matrix

    Finely Granular fine dark purple granules in


    Inclusion Casts pale pink or pale purple matrix

    Waxy Casts pale pink or pale purple Darker than hyaline casts,
    but of a pale even color.
    Distinct broken ends.

    Fat Inclusion Cast fat globules unstained in a Rare. Presence is con-


    pink matrix firmed if examination
    under polarized light
    indicates double
    refraction.

    Red Cell pink to orange-red Intact cells can be seen


    Inclusion Cast in matrix

    Blood (Hemoglobin) orange-red No intact cells


    Casts

    Bacteria motile: don’t stain Motile organisms are not


    non-motile: stain purple impaired.

    Trichomonas light blue green Motility is unimpaired in


    fresh specimens when
    recommended volumes
    of stain are used.
    Immotile organisms are
    also indentifiable.

    Mucous pale pink or pale blue

    Background pale pink or pale purple

    LIMITATIONS
    Microscopic examination of urinary sediment is a semi-quantitative procedure.
    In cases where exact count of leukocytes, bacteria, cast, etc., are required,
    techniques employing a hemocytometer are preferred.

    EXPECTED VALUES
    Some erythrocytes, leukocytes and casts are excreted by normal individuals,
    but they are seen only occasionally in urinary sediments examined
    microscopically.
    Two to three red blood cells, 4-5 leukocytes per high powered field and
    occasional hyaline casts are accepted as normal.

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