Sigma Centrifuge 4-16 Manual
Sigma Centrifuge 4-16 Manual
Sigma Centrifuge 4-16 Manual
Centrifuge
4-16
Serial number:
Copyright by
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany
for service
or contact:
Phone +49 (0) 55 22 / 50 07-84 25
Fax +49 (0) 55 22 / 50 07-94 25
E-mail: service@sigma-zentrifugen.de
Table of Contents
Table of Contents
1 General Information 9
1.1 Importance of the Operating Manual .......................................................................... 9
1.2 Intended Use .............................................................................................................. 9
1.3 Warranty and Liability ................................................................................................. 9
1.4 Copyright.................................................................................................................. 10
1.5 Standards and Regulations ...................................................................................... 10
1.6 Scope of Supply ....................................................................................................... 10
3 Safety 13
3.1 Marking of the Unit ................................................................................................... 13
3.2 Explanation of the Symbols and Notes ..................................................................... 14
3.3 Responsibility of the Operator .................................................................................. 15
3.4 Operating Personnel................................................................................................. 15
3.5 Informal Safety Instructions ...................................................................................... 15
3.6 Safety Instructions.................................................................................................... 16
3.6.1 Electrical Safety .......................................................................................... 16
3.6.2 Mechanical Safety ...................................................................................... 16
3.6.3 Fire prevention............................................................................................ 17
3.6.4 Chemical and Biological Safety................................................................... 17
3.6.5 Safety Instructions for Centrifugation .......................................................... 17
3.6.6 Resistance of Plastics................................................................................. 18
3.6.7 Service Life of Rotors and Accessories....................................................... 18
3.7 Safety Devices ......................................................................................................... 19
3.7.1 Lid Lock Device .......................................................................................... 19
3.7.2 Standstill Monitoring.................................................................................... 19
3.7.3 System Check............................................................................................. 19
3.7.4 Ground Wire Check .................................................................................... 19
3.7.5 Imbalance Monitoring System..................................................................... 20
3.8 Measures in the Event of Hazards and Accidents..................................................... 20
3.9 Remaining Hazards .................................................................................................. 20
Table of Contents
Table of Contents
Table of Contents
9 Disposal 67
9.1 Disposal of the Centrifuge ........................................................................................ 67
9.2 Disposal of the Packaging ........................................................................................ 67
10 Technical Data 68
10.1 Ambient Conditions .................................................................................................. 68
11 Appendix 69
11.1 Suitable Accessories ................................................................................................ 69
11.1.1 Maximum speed for tubes........................................................................... 79
11.2 Grafical Representation of the Rotors....................................................................... 79
11.3 Acceleration and Deceleration Curves...................................................................... 81
11.3.1 Linear Curves ............................................................................................. 81
11.3.2 Quadratic Curves ........................................................................................ 82
11.4 Formulae - Mathematical Relations .......................................................................... 83
11.4.1 Relative Centrifugal Force (RCF) ................................................................ 83
11.4.2 Density........................................................................................................ 83
11.4.3 Speed-Gravitational-Field-Diagram ............................................................. 83
Table of rotors and accessories with a different service life ...................................... 85
Resistance Data ....................................................................................................... 86
EC-Declaration of Conformity ................................................................................... 90
12 Index 91
General Information
1 General Information
General Information
1.4 Copyright
The copyright concerning the operating manual remains Sigma
Laborzentrifugen GmbH.
The operatin manual is solely intended for the operator and their
personnel. It includes instructions and information that may not be
• duplicated,
• distributet, or
• communicated in any othe way
neither in full nor in parts.
Non-compliance may be prosecuted under criminal law.
Documentation:
Operating Manual incl. EC Declaration of Conformity (page 90)
Accessories
according to your order, our order confirmation, and your delivery
note.
8 Name plate
(see page 12)
9 Mains power input
10 Equipotential
bonding screw
9
10
Safety
3 Safety
3.1 Marking of the Unit
International symbols used for SIGMA centrifuges:
Ein (Netzverbindung)
I On (Power)
Marche (mise sous tension)
Aus (Netzverbindung)
O Off (Power)
Arrêt (mise hors tension)
Schutzleiteranschluss
Protective earth (ground)
Liaison à la terre
Erde
Earth (ground)
Terre
Netzstecker ziehen
Unplug mains plug
Tirer la fiche de prise
Vorsicht Quetschgefahr
Caution! Risk of bruising
Attention! Danger de blessure
→
Drehrichtungspfeil
Arrow direction of rotation
Flèche sens de rotation
Heiße Oberfläche
Hot surface
Surface chaude
Safety
This symbol stands for a direct hazard to the life and health of
persons due to electrical voltage.
This symbol stands for a potential hazard to the life and health of
persons.
Safety
Safety
Safety
Safety
Safety
Safety
Height: 489 mm
Width: 496 mm
Depth: 650 mm
Weight: 82 kg
Fig. 4.1: Dimensions and weight
Removal:
• Lift the centrifuge upwards at the front side. Always reach under
from the side.
• Put a suitable object, e.g. a wooden block, between table and
centrifuge. Two plastic screws can now be seen at the bottom
panel.
• Unscrew the screws by hand anti-clockwise.
• Retain the transport safety device for the possibility of the return
of the centrifuge.
The operating voltage on the name plate must correspond to the local
supply voltage!
5.4.2 Fuses
Typically, the centrifuges must be protected on-site with 16 Amp L or
B fuses.
• Do not hold your fingers between the lid and the housing when
closing the lid. Risk of crushing!
When using rotors for microtiter plate formats: Ensure that the plate
holders are inserted together with the plates into the bucket.
• The lid screw serves for the fastening of the lid onto the rotor
only, not for the fastening of the rotor onto the motor shaft.
• Follow the safety instructions and hazard warnings in chapter 3!
24 Version 11/2008, Rev. 1.11 of 01/09/2011 - sb
Translation of the original operating manual
4-16
Fig. 6.2:
Permissible loading of the swing-
out rotor with different tube sizes
Fig. 6.3:
Permissible loading of the
swing-out rotor
6.2.2.4 Adapters
In order to ensure easy handling, even if vessels of various sizes are
used, carrier systems were developed.
• Load the opposite carriers with the same number of vessels and
with the same weights in order to avoid imbalance.
• If all of the compartments of a carrier are not used, the buckets
must be loaded evenly. Loading the edges of a bucket only is not
permissible.
6.2.2.5 Tubes
• Load the tubes outside of the centrifuge. Liquids in the buckets or
multiple carriers cause corrosion .
• Fill the tubes carefully and arrange them according to their
weight. Imbalances result in the excessive wear of the bearings.
• In high-speed angle rotors, the vessels must be filled up to their
useful volume (= the volume stated for the vessel). If the vessels
are only partially filled, they will deform. This may result in leaks
at the seals that may become loose.
• When using glass tubes, the maximum value of 4,000 x g must
not be exceeded (except special high-strengh glass tubes;
please refer to the information provided by the manufacturer).
• Observe the instructions on safety and hazards in chapter 3!
1 Centrifuge display
2 Functional key
3 Start key
4 Stop key
5 Lid key
1 2 3 4 5
The centrifuge is started directly via the operating panel. When the
centrifuge is switched on, all of the operating keys and displays will
be illuminated for a short time. It is now ready for operation.
1 2
1 Speed field
2 RCF field
3 Time field 3
4 Program field
5 Rotor field
6 Parameters
7 Configurations
4 5 6 7
All entries beyond the permissible limits, or incorrect entries, will not
be accepted.
Speed
In the upper section of the field, the set speed of the centrifuge is
displayed. The actual speed is displayed below this value. The values
are stated in revolutions per minute (rpm) and depend on the RCF
values (see 11.4 "Formulae – Mathematical Relations "). The
maximum speed values depend on the rotor that is used.
Time
The set runtime is displayed in the upper section of this field, with the
remaining runtime shown below. The runtime is defined as the period
from the start of the centrifuge to the beginning of the deceleration
phase. The maximum value is 9 h 59 min. The unit of the
programmed centrifugation time is underlined.
The set value is indicated in hours, minutes, and seconds. The actual
value has the same units as the set value and is displayed in
hours:minutes, or in minutes:seconds if the value is below 10
minutes. Values greater than 59 will be automatically converted to the
next higher unit.
Continuous Run
During the continuous run, the runtime of the centrifuge is unlimited
and must be stopped manually. The centrifuge accelerates during the
continuous run until the set speed is reached.
To start the continuous run of the centrifuge :
• Starting with the time setting 0:10 (see page 29), turn the knob
counter-clockwise or
• Starting with the time setting 09:59, turn the knob clockwise to the
next setting. "HOLD” will be displayed. After the start of the
centrifuge, the elapsed time will be displayed.
Short Run
• Keep the start key pressed during the short run.
During the short run, the centrifuge accelerates with the maximum
acceleration curve until the maximum speed of the rotor is reached.
When the start key is released, the centrifuge decelerates at
maximum brake to a standstill.
Program
In this field, the number of the actual program is displayed.
• Select the double arrow ( ) next to the program list and confirm
the entry. An overview of all stored programs will be displayed
(see 6.3.4 "Program Mode").
Acceleration
This function is used to select an acceleration curve. One can select
a linear rise (curves 0-9) or a quadratic rise (curves 10-19). The
acceleration curves 20-29 can be programmed as desired.
Their shape is explained in detail in 11.3.1 "Linear Curves" and 11.3.2
"Quadratic Curves".
Deceleration
This function is used in order to select a curve that decelerates the
centrifuge to a standstill. Deceleration curves are inverted images of
the acceleration curves and are labelled with identical numbers.
Deceleration curve no. 0 represents a brakeless deceleration.
Radius
The radius determines the value of the relative centrifugal force
(RCF) that the sample is subjected to. Normally, the maximum RCF
value is displayed. If the value is reduced manually, a downward
facing arrow (↓) will be displayed in the RCF field.
Density
If the density of the liquid is higher than 1.2 g/cm3, the value must be
adapted manually. This, in turn, reduces the maximum final speed
(see 11.4.2 “Density“). A downward facing arrow (↓) will be displayed
in the speed field. Values between 1.2 and 9.9 g/cm3 are possible.
Start Delay
If the start delay is activated, the centrifuge will not start until the
preset time has elapsed.
Code
In order to prevent any unauthorised use of the centrifuge, the
following functions can be blocked:
• Saving of programs
• Changing of parameters
• Loading of programs
• Start key
Blocking a function:
• Select the function that is to be blocked.
• Select the button "Activate code".
• Enter a four-digit code and confirm the entry.
• For safety reasons, the code must be entered a second time.
The blocking is now active.
If changes are made to a blocked function, the system will ask for the
code prior to executing the change.
Unblocking a function
• Select the button "Delete code".
• Enter the code and confirm the entry.
The function is now unblocked.
Language
The German or English languages can be selected as the menu
language.
Screen
Various representation variants enable a zoomed representation of
the display:
- RCF and speed in the standard size
- Speed enlarged (zoomed). The RCF display is eliminated.
- RCF enlarged (zoomed). The speed display is eliminated.
• Select the “Display” field in the configuration overview and confirm
the selection. The display overview will be displayed.
• In order to set the display "SPEED - (ZOOM)“, select the
corresponding line and confirm the selection.
• Quit the display by selecting and confirming “EXIT”. The system
will return to the configuration overview.
After another confirmation, the standard menu will be displayed. The
fields "SPEED“ and "TIME“ are zoomed. The field “RCF” is
eliminated.
Fine Adjustment
This menu can be used to preselect the set speed in steps of 1 or 10
rpm, and the set time in steps of 1 min or 1 sec.
Cycles
For each rotor/bucket combination, the number of cycles and runtime
are stored.
Buzzer
In this menu, a sound signal can be selected for
• the end of a centrifugation run
• an imbalance message
• an error message.
The duration of the buzzer signal can be specified.
Sensor
The sensor overview shows various system data. In the event of a
malfunction, these data facilitate an immediate diagnosis.
Info
The Info overview shows information such as the type of centrifuge,
and EPROM version, number of cycles, the total runtime, as well as
software version and date.
In the menus "Cycles", “Info“, and “Sensor“, the values can neither be
entered nor changed.
Reset
This function resets the centrifuge and restores the factory settings.
All of the programs, parameters, curves, and configurations will be
deleted.
Curves
In this submenu, the user-defined acceleration and deceleration
curves can be created and edited (see 6.3.3.8).
• Activate the time display of the second interval and enter the
desired time. The extended curve will be displayed.
• Select the speed field "SPEED" and enter a speed value. Confirm
the entry.
• You can enter up to 10 intervals in this manner.
• Save the curve under a curve number between 20 and 29
together with the current operating parameters.
• Select “EXIT” and quit the curve mode by confirming the entry.
Only acceleration curves are programmed. The corresponding
deceleration curves are generated by reflection.
If the curve speed of an interval exceeds the set speed in the
operating mode, a dialog box will pop up.
Centrifugation curves
It is also possible to create centrifugation curves. The following
conditions must be fulfilled:
• The preselected maximum final speed of an interval corresponds
to the set speed for the centrifugation run.
• The sum of the intervals corresponds to the set time of the centri-
fugation run. The last speed checkpoint of an interval equals 0.
Deceleration curve
1 Start key
2 Centrifuge display
3 Function knob
4 Stop key
5 Lid key
1 2 3 4 5
1 Menu bar 1
2 Speed field 7
2
3 RCF field
4 Rotor field
5 Program field
8
6 Status bar 3
7 Runtime field
8 Acceleration
9 Deceleration (brake) 4 9
5 10
6
Fig. 6.27: Centrfuge display Spincontrol Professional
Fast stop:
• Press the stop key for more than three seconds.
The centrifuge decelerates with the maximum deceleration curve.
After a fast stop, the centrifuge lid must be opened before a new
centrifugation run can be started.
A fast stop can also be triggered during a normal deceleration, e.g. in
order to speed up the deceleration.
When a fast stop is triggered, “Fast stop” will be displayed in the
speed field.
This menu is displayed a few seconds after the centrifuge has been
switched on.
The following parameters can be displayed and modified:
Speed
In the upper section of the field, the set speed of the centrifuge is
displayed. The actual speed is displayed below this value. The values
are stated in revolutions per minute (rpm) and depend on the RCF
values (see 11.4 "Formulae – Mathematical Relations "). The
maximum speed values depend on the rotor that is used.
Runtime
The set runtime is displayed in the upper section of this field, with the
remaining runtime shown below. The runtime is defined as the period
from the start of the centrifuge to the beginning of the deceleration
phase. The maximum value is 99 h 59 min 59 sec.
In the “Setup” menu, one can specify that the counting of the runtime
is not to be started until the set speed is reached (see 6.4.3.8 "Setup
Menu"). In this case, the symbol "!" appears in the speed field.
Continuous Run
During the continuous run, the runtime of the centrifuge is unlimited
and must be stopped manually. The centrifuge accelerates during the
continuous run until the set speed is reached.
• Select the field “Runtime” and confirm the selection. The display
flashes when it is activated
• Select the time 0:00:00 and confirm the selection. "Infinite" will be
displayed. After the start of the centrifuge, the elapsed time will be
displayed.
• Deactivate the continuous run by pressing the stop key or by
entering a specific run time.
Short Run
• Keep the start key pressed during the short run.
During the short run, the centrifuge accelerates with the maximum
acceleration curve until the maximum speed of the rotor is reached.
When the start key is released, the centrifuge decelerates at
maximum brake to a standstill.
Acceleration
This function is used to select an acceleration curve. One can select
a linear rise (curves 0-9) or a quadratic rise (curves 10-19). The
acceleration curves 20-29 can be programmed as desired.
Their shape is explained in detail in 11.3.1 "Linear Curves" and 11.3.2
"Quadratic Curves".
Deceleration (Brake)
This function is used in order to select a curve that decelerates the
centrifuge to a standstill. Deceleration curves are inverted images of
the acceleration curves and are labelled with identical numbers.
Deceleration curve no. 0 represents a brakeless deceleration.
Process
Radius
The radius determines the value of the relative centrifugal force
(RCF) that the sample is subjected to. Normally, the maximum RCF
value is displayed. If the value is reduced manually, a downward
facing arrow (↓) will be displayed in the RCF field.
Density
If the density of the liquid is higher than 1.2 g/cm3, the value must be
adapted manually. This, in turn, reduces the maximum final speed
(see 11.4.2 “Density“). A downward facing arrow (↓) will be displayed
in the speed field. Values between 1.2 and 9.9 g/cm3 are possible.
Lock
In order to prevent any unauthorised use of the centrifuge, the
following functions can be blocked:
• Saving of programs
• Changing of parameters
• Loading of programs
• Start key
Blocking a function:
• Select the function that is to be blocked. The preceding functions
will also be automatically selected (example: If the Parameter
function is selected, the Save function will also be selected).
• Select the button "Activate code".
• Enter a four-digit code and confirm the entry.
The blocking is now active. A padlock symbol " " will be displayed in
the status line.
If changes are made to a blocked function, the system will ask for the
code prior to executing the change.
Unblocking a function
• Select the button "Deactivate code".
• Enter the code and confirm the entry.
The function is now unblocked.
Curve Editor
The Curve Editor function can be used to create and edit user-
specific acceleration and deceleration curves.
General information concerning this subject can be found in chapter
11.3 "Acceleration and Deceleration Curves".
Restrictions
• Deceleration and acceleration curves may include intervals with a
positive slope as well as with a zero slope.
• The slop of the curve intervals may be between 4 rpm min. and
1000 rpm max.
• Square curve intervals are only possible between 0 and 1000 rpm
max. If the selected final speed is higher than 1000 rpm, this
interval will automatically turn into a linear one above 1000 rpm.
The possible runtime depends on the maximum possible speed
(depending on the rotor) and the slop limitation.
Function
Open lid after run
The Auto-Lid-Open function must be activated so that the lid opens
automatically at the end of the operation.
Program rotation
See chapter 6.4.4.4 "Automatic program rotation"
Start delay
If the start delay function is active, the centrifuge will not start until the
preset time has elapsed. The symbol "" will be displayed in the time
field.
Display
Language
Various languages are available.
If a language is selected by mistake, it can be changed on any screen
as follows:
• Press and hold the stop key.
• Turn the function knob one notch to the left and then one notch to
the right.
• Release the stop key. The window "Language" will be displayed.
• Select the desired language.
Invert
This function switches the display from black/white to white/black.
Buzzer
In this menu, a sound signal can be selected for
• the end of a centrifugation run
• an imbalance message
• an error message.
The duration of the buzzer signal can be specified.
External
This function is only available if the centrifuge is equipped with the
option for the input and output of data (external signal, floating
switch) (see 6.4.4.5).
System
The following system data are displayed:
• Cycles
• Info
• Sensor
In the menus "Cycles", “Info“, and “Sensor“, the values can neither be
entered nor changed.
Parameter changes concerning the speed, RCF, and time do not refer
to acceleration or deceleration processes, but rather to the run phase.
The current program is loaded and can be started. After the end of
the run, the next program will be loaded and started automatically.
The rotation ends with the first empty location and restarts from the
beginning:
Example 1: Loading of sample 1
Rotation: sample 1, sample 2, sample 3, sample 1,...
Example 2: Loading of sample 2
Rotation: sample 2, sample 3, sample 2,...
Lid cannot be opened Lid lock has not released Unlock the lid manually (see
7.1.1 "Emergency Lid Release")
and contact service
Lid seal sticks Clean the lid seal and apply
talcum powder
Hard running noise during the Screws of the transport safety Remove screws of the transport
centrifugation device are not removed safety device (see 5.2)
Fig. 7.1: Possible error causes
Fig. 7.2: Position of the opening for the emergency lid release
• Insert the supplied hexagon socket key horizontally into the hole.
The key will be guided through a funnel-shaped tube to the shaft
of the lid lock motor.
Fig. 7.3: The emergency lid release key must be inserted horizontally
(similar to illustration)
• Unlock the motorised lid lock with the supplied hexagon socket
key (size 5) by turning it anti-clockwise.
• Then, reinsert the plug.
58 Version 11/2008, Rev. 1.11 of 01/09/2011 - sb
Translation of the original operating manual
4-16
If the lid is opened via the emergency lid release system during a
centrifuge run, the centrifuge will be switched off immediately and
decelerate in an unbraked manner.
from Germany:
• use the service request form at
www.sigma-zentrifugen.de → [Service Area]
• or contact
outside Germany:
contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de → [Contacts] → [Foreign agencies]
• If you would like to utilise our service, please state the type of
your centrifuge and its serial number.
• Make use of our service request on the Internet. Please use the
request form (see above) on our website.
8.1 Maintenance
8.1.1 Centrifuge
• Disconnect the power cord from the wall outlet or instrument
receptacle before cleaning.
• Carefully remove all liquids, including water and particularly all the
solvents, acids, and alkaline solutions from the rotor chamber
using a cloth in order to avoid damage to the motor bearings.
• If the centrifuge has been contaminated with toxic, radioactive, or
pathogenic substances, clean the rotor chamber immediately with
a suitable decontamination agent (depending on the type of
contamination).
• Grease the motor shaft slightly after cleaning (Grease for load-
bearing bolts part no. 70284).
8.1.2 Accessories
For the care of the accessories, special safety measures must be
considered as these are measures that will ensure operational safety
at the same time.
In order to completely remove the glass particles and the metal dust
from the rotor chamber:
• Grease the upper third of the rotor chamber with e.g. Vaseline.
• Then, let the rotor rotate for a few minutes at a moderate speed
(approximately. 2,000 rpm). The glass and metal particles will
now collect at the greased part.
• Remove the grease with the glass and metal particles with a cloth.
• If necessary, repeat this procedure.
8.2.1. Autoclaving
The service life of the accessories essentially depends on the
frequency of autoclaving and use.
• Replace the accessories immediately when the parts show
changes in color or structure or in the occurrence of leaks etc.
• During autoclaving, the caps of the tubes must not be screwed on
in order to avoid the deformation of the tubes.
It cannot be excluded that plastic parts, e.g. lids or carriers, may
deform during autoclaving.
Accessories max. temp.(°C) min.time max. time max.cycles
Glass tubes 134-138 3 40 -
Polycarbonate tubes 115-118 30 40 20
Polypropylene copolymer tubes 115-121 30 40 30
Teflon tubes 134-138 3 5 100
Aluminium rotors 134-138 3 20 -
Polycarbonate/Polyallomer lids for angle rotors 115-118 30 40 20
Polysulfone lids for angle rotors 134-138 3 5 100
Aluminium buckets 134-138 3 20 -
Polysulfone caps for buckets 134-138 3 5 100
Rubber adapter 115-118 30 40 -
Round carriers made of polypropylene 115-118 30 40 -
ditto, made of polyallomer and polycarbonate 115-118 30 40 -
Rectangular carriers made of polypropylene 115-118 30 40 -
ditto, made of polyallomer and polycarbonate 115-118 30 40 -
Fig. 8.1: Autoclaving table
8.3 Service
In the event of service work that requires the removal of the panels,
there is a risk of electric shock or mechanical injury. Only qualified
specialist personnel is authorised to perform this service work.
in Germany:
• use the service request form at
www.sigma-zentrifugen.de → [Service Area]
• or contact
outside Germany:
contact our agency in your country. All agencies are listed at
www.sigma-zentrifugen.de → [Contacts] → [Foreign agencies]
• If you would like to utilise our service, please state the type of
your centrifuge and its serial number.
• Make use of our service request on the Internet. Please use the
request form (see above) on our website.
1. Declaration of decontamination
As a certified company and due to the legal regulations for the
protection of our employees and of the environment, we are
obliged to certify the harmlessness of all incoming goods. For this
purpose, we require a declaration of decontamination.
• The form must be filled in completely and signed by authorised
and specialised personnel only.
• Affix the original form in a clearly visible manner to the outside
of the packaging.
Disposal
9 Disposal
9.1 Disposal of the Centrifuge
In accordance with the directive 2002/96/EC, SIGMA centrifuges are
marked with the symbol shown to the left. This symbol means that it
is not permissible to dispose of the unit among household trash.
• You can return these centrifuges free of cost to SIGMA
Laborzentrifugen GmbH.
• Ensure that the unit is decontaminated. Fill in a declaration of
decontamination.
• Comply with any other applicable local rules and regulations.
Technical Data
10 Technical Data
Manufacturer: S I G M A Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode
Germany
Type: 4-16
Electr. Connection: see name plate
Protection class: I
IP Code: 20
Connected load (kVA): 1,5
Power consumption (kW): 1,7
Max. current consumption (A): 6.5 (at 22 -240 V / 50-60 Hz)
13.0 (at 120 V / 60 Hz)
Performance data
Max. speed (rpm): 13 500
Max. capacity (ml): 2 600
Max. gravitational field (x g): 20 376
Max. kin. energy (Nm): 49 543
Other parameters
Time range: short run, continuous run,
Spincontrol Comfort 10 sec – 9 h 59 min
Spincontrol Professional 10 sec – 99 h 59 min
Storage locations:
Spincontrol Comfort 50
Spincontrol Professional 60
Physical data
Height (mm): 489
Height with opened lid (mm) : 940
Width (mm): 496
Depth (mm): 650
Weight (kg): 82
EMC as per EN 61326: Class B
Noise level (dBA): 73 (at max. speed)
Fig. 10.1: Technical Data
∗
At a nominal voltage of 100V or 200V, a tolerance of +10% / -5% applies.
Appendix
11 Appendix
11.1 Suitable Accessories
Part no. Description Max. speed Max.
(rpm) gravitational
field (x g)
11650 Swing-out rotor, 4 place, for buckets 13421, 13450 4 200
13421 Bucket for microtiter- and deepwellplates, , incl. plate
holder no. 17979
Radius max. 15,7 cm 4 200 3 096
Radius min. 9,4 cm 1 854
max. height of plates 60 mm
1 set = 4 pcs.
13450 Round bucket, sealable with cap no. 17170, for the
round carrier system Ø 100, (no. 17649 - 17699), for 650
ml tube no. 13456 , adapter no. 13654, max. tube length 3 708
140 mm, 1 set = 2 pcs. 4 200
17653 Round carrier for 26 reaction vials 1.5/2.2 ml,
max. Ø 11 mm, e.g. no. 15008, 15040, 1 set = 2 pcs.
17651 Round carrier for 20 tubes 4 - 7 ml, flat bottom,
max. Ø 15.5/18 x 50 - 75 mm, e.g. Monovette,
Primavette, 1 set = 2 pcs.
17652 Round carrier for 36 round bottom tubes 5 ml,
max. Ø 12.5 x 60 - 80 mm, e.g. no. RIA tube 15060,
hemolyse tubes, 1 set = 2 pcs.
17656 Round carrier for 22 round bottom tubes 5 - 6 ml,
max. Ø 13.5/17.5 x 70 - 90 mm, e.g. no. RIA tube 15060
Vacutainer -, hemolyse tubes, 1 set = 2 pcs.
17654 Round carrier for 32 round bottom tubes approx. 7 ml,
max. Ø 12.5 x 85 - 115 mm, e.g. no. 15007, 15027,
1 set = 2 pcs.
17658 Round carrier for 19 round bottom tubes 9 - 15 ml,
max. Ø 17.2/19.5 x 85 - 115 mm, e.g. no. 15015, 15020,
15022, 15023, 15024 and Monovettes 9 - 10 ml,
1 set = 2 pcs.
17655 Round carrier for 20 round bottom tubes 10 - 12 ml,
max. Ø 16.2/18 x 65 - 90 mm, e.g. no. 15000, 15010,
15039, 1 set = 2 pcs.
17659 Round carrier for 12 culture tubes 15 ml no. 15115
Ø max. 23 mm, 1 set = 2 pcs.
17660 Round carrier for 15 culture tubes 15 ml
with caps Ø max. 22 mm, 1 set = 2 pcs.
17670 Round carrier for 8 round bottom tubes 25 - 30 ml,
max. Ø 25.4/30 x 90 - 115 mm, e.g. no. 15025, 15026,
15029, 15030, 15032, 15033, 1 set = 2 pcs.
17662 Round carrier for 7 tubes 30 ml, flat bottom or skirt,
max. Ø 25/31 x 65 - 95 mm, e.g. Sterilin tube 30 ml,
Barloworld Scientific Ltd., 1 set = 2 pcs.
Appendix
Appendix
Appendix
Appendix
Appendix
Appendix
Appendix
Appendix
Appendix
Further Accessories
Part no. Description
16018 Rubber adapter for special glass tube 15 ml no. 15022 and for glass tube no. 15085,
1 set = 2 pcs.
Appendix
Appendix
Fig 11.1:
Minimum and maximum radius of
a swing-out rotor
Fig. 11.2:
Minimum and maximum radius of
an angle rotor
Appendix
Linear Curves
98 7 6 5 4 3 2 1 0
1000
900
curve 0
800
curve 1
700 curve 2
curve 3
Speed [rpm]
600
curve 4
500
curve 5
400 curve 6
300 curve 7
curve 8
200
curve 9
100
0
0 50 100 150 200 250
Time [sec]
Appendix
Quadratic Curves
18
19 17 16 15 14 13 12 11 10
1000
900
curve 10
800
curve 11
700 curve 12
600 curve 13
Speed [rpm]
curve 14
500
curve 15
400 curve 16
300 curve 17
curve 18
200
curve 19
100
0
0 100 200 300 400 500
Time [sec]
Appendix
Quadratic curve no. Time until 1,000 rpm Slope as of 1,000 rpm
10 500 sec 4 [rpm / sec]
11 333 sec 6 [rpm / sec]
12 250 sec 8 [rpm / sec]
13 118 sec 17 [rpm / sec]
14 80 sec 25 [rpm / sec]
15 60 sec 33 [rpm / sec]
16 40 sec 50 [rpm / sec]
17 20 sec 100 [rpm / sec]
18 10 sec 200 [rpm / sec]
19 2 sec 1000 [rpm / sec]
Fig. 11.6: Slope of quadratic curves
11.4.2 Density
If the density of the liquid is higher than 1.2 g/cm3, the maximum
permissible speed of the centrifuge is calculated according to the
following formula:
n = nmax x (1.2 / Rho)
Rho = density in g/cm3
11.4.3 Speed-Gravitational-Field-Diagram
Additional help can be found in the enclosed speed-gravitational-field-
diagram (see fig. 11.7, next page).
Appendix
Appendix
If other no data concerning the service life are engraved on the rotor or accessory, rotors
and buckets must be checked by the manufacturer after 10,000 cycles. After 50,000 cycles,
rotors must be scrapped for safety reasons.
Appendix
Resistance Data
Resistant at +20 °C
Concentration
Polyamide
Polycarbonate
Polyoxymethylene
Polypropylene
Polysulfone
Polytetrafluorethylene
Aluminum
- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant
HDPE
PTFE
POM
PSU
PVC
PVC
PC
PA
PP
AL
Medium Formula [%]
Acetaldehyde C2H4O 40 3 2 4 2 3 4 4 - 1 1
Acetamide C2H5NO saturated 1 1 4 1 1 4 4 - 1 1
Acetone C3H6O 100 1 1 4 1 1 4 4 - 1 1
Acrylonitrile C3H3N 100 1 1 4 3 3 4 4 4 1 1
Allyl alcohol C3H6O 96 1 3 3 2 2 2 2 4 1 1
Aluminum chloride AlCl3 saturated 1 3 2 4 1 - 1 - 1 4
Aluminum sulfate Al2(SO4)3 10 1 1 1 3 1 1 1 1 1 1
Ammonium chloride (NH4)Cl aqueous 1 1 1 2 1 1 1 1 1 3
Ammonium hydroxide NH3 + H2O 30 1 3 4 1 1 2 1 - 1 1
Aniline C6H7N 100 1 3 4 1 2 4 4 4 1 1
Anisole C7H8O 100 3 4 4 1 4 4 2 - 1 1
Antimony trichloride SbCl3 90 1 4 1 4 1 - 1 - 1 4
Benzaldehyde C7H6O 100 1 3 4 1 1 3 4 4 1 1
Benzene C6H6 100 3 2 4 1 3 4 4 - 1 1
Boric acid H3BO3 aqueous 1 3 1 2 1 - - - 1 1
Butyl acrylate C7H12O2 100 1 2 4 2 3 4 4 4 1 1
Butyl alcohol, normal C4H10O 100 1 1 2 1 1 2 2 4 1 1
Calcium chloride CaCl2 alcoholic 1 4 2 3 1 - - 4 1 3
Carbon disulfide CS2 100 4 3 4 2 4 4 4 4 1 1
Carbon tetrachloride (TETRA) CCl4 100 4 4 4 2 4 4 4 4 1 1
Chlorine Cl2 100 4 4 4 4 4 4 4 4 1 3
Chlorine water Cl2 x H2O 3 4 4 4 3 - 3 3 1 4
Chlorobenzene C6H5Cl 100 3 4 4 1 3 4 4 4 1 1
Chloroform CHCl3 100 3 3 4 4 3 4 4 4 1 3
Chromic acid CrO3 10 1 4 2 4 1 4 1 - 1 1
KCr(SO4)2 x
Chromic potassium sulfate saturated 1 2 1 3 1 - 1 - 1 3
12H2O
Citric acid C6H8O7 10 1 1 1 2 1 1 1 1 1 1
Citric acid C6H8O7 50 1 3 1 2 1 - - - 1 1
Copper sulfate CuSO4 x 5H2O 10 1 1 1 1 1 1 1 1 1 4
Cyclohexanol C6H12O 100 1 1 3 1 1 1 1 4 1 1
Decane C10H22 100 - 1 2 1 3 - - - 1 1
Diaminoethane C2H8N2 100 1 1 3 1 1 - 3 4 1 1
Diesel fuel — 100 1 1 3 1 1 - 1 3 1 1
Dimethyl formamide (DMF) C3D7NO 100 1 1 4 1 1 4 3 - 1 1
Dimethyl sulfoxide (DMSO) C2H6SO 100 1 2 4 1 1 4 4 - 1 1
Dimethylaniline C8H11N 100 - 3 4 2 4 - - - 1 1
Appendix
Resistant at +20 °C
Concentration
Polyamide
Polycarbonate
Polyoxymethylene
Polypropylene
Polysulfone
Polytetrafluorethylene
Aluminum
- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant
HDPE
PTFE
POM
PSU
PVC
PVC
PC
PA
PP
AL
Medium Formula [%]
Dioxane C4H8O2 100 2 1 4 1 3 2 3 4 1 1
Dipropylene glycol
C4H10O 100 3 1 4 1 4 4 4 4 1 1
(mono)methyl ether
Ethyl acetate C4H8O2 100 1 1 4 1 1 4 4 4 1 1
Ethylene chloride C2H4Cl2 100 3 3 4 1 3 4 4 4 1 1
Ferrous chloride FeCl2 saturated 1 3 1 3 1 1 1 1 1 4
Formaldehyde solution CH2O 30 1 3 1 1 1 - - - 1 1
Formic acid CH2O2 100 1 4 3 4 1 3 3 1 1 1
Furfural C5H4O2 100 1 3 3 2 4 - - - 1 1
Gasoline C5H12 - C12H26 100 2 1 3 1 3 3 2 - 1 1
Glycerol C3H8O3 100 1 1 3 1 1 1 1 2 1 1
Heptane, normal C7H16 100 2 1 1 1 2 1 2 4 1 1
Hexane, n- C6H14 100 2 1 2 1 2 1 2 4 1 1
Hydrogen chloride HCl 5 1 4 1 4 1 1 1 - 1 4
concentrat
Hydrogen chloride HCl 1 4 4 4 1 1 2 3 1 4
ed
Hydrogen peroxide H2O2 3 1 3 1 1 1 1 1 - 1 3
Hydrogen peroxide H2O2 30 1 4 1 4 1 1 1 - 1 3
Hydrogen sulfide H2S 10 1 1 1 1 1 1 1 3 1 1
Iodine, tincture of I2 1 4 3 1 1 - 4 4 1 1
Isopropyl alcohol C3H8O 100 1 1 1 1 1 1 1 4 1 2
Lactic acid C3H6O3 3 1 3 1 2 1 1 2 - 1 1
Magnesium chloride MgCl2 10 1 1 1 1 1 1 1 1 1 1
Mercuric chloride HgCl2 10 1 4 1 3 1 1 1 1 1 4
Mercury Hg 100 1 1 1 1 1 1 1 3 1 3
Methyl acetate C3H6O2 100 1 1 4 2 1 - 4 4 1 1
Methyl alcohol CH4O 100 1 2 4 1 1 3 1 3 1 1
Methyl benzene C7H8 100 3 1 4 1 3 4 4 4 1 1
Methyl ethyl ketone (MEK) C4H8O 100 1 1 4 1 1 4 4 4 1 1
Methylene chloride CH2Cl2 100 4 3 4 3 3 4 4 4 1 1
Mineral oil — 100 1 1 1 1 1 1 1 - 1 1
Nitric acid HNO3 10 1 4 1 4 1 1 1 - 1 3
Nitric acid HNO3 100 4 4 4 4 4 - 4 - 1 1
Nitrobenzene C6H5NO2 100 3 4 4 3 2 4 4 4 1 1
Oleic acid C18H34O2 100 1 1 1 2 1 - 1 - 1 1
Oxalic acid C2H2O4 x 2H2O 100 1 3 1 4 1 1 1 1 1 1
Ozone O3 100 3 4 1 4 3 1 1 - 1 2
Petroleum — 100 1 1 3 1 1 1 1 3 1 1
Phenol C6H6O 10 1 4 4 4 1 4 1 3 1 1
Phenol C6H6O 100 2 4 4 4 1 3 4 3 1 1
Phosphoric acid H3PO4 20 1 4 2 4 1 - - - 1 4
Phosphorus pentachloride PCl5 100 - 4 4 4 1 - 4 4 1 1
Appendix
Resistant at +20 °C
Concentration
Polyamide
Polycarbonate
Polyoxymethylene
Polypropylene
Polysulfone
Polytetrafluorethylene
Aluminum
- no data
1 resistant
2 practically resistant
3 partially resistant
4 not resistant
HDPE
PTFE
POM
PSU
PVC
PVC
PC
PA
PP
AL
Medium Formula [%]
Potassium hydrogen carbonate CHKO3 saturated 1 1 2 1 1 - - - 1 4
Potassium hydroxide KOH 30 1 1 4 3 1 1 1 1 1 4
Potassium hydroxide KOH 50 1 1 4 3 1 1 1 1 1 4
Potassium nitrate KNO3 10 1 1 1 1 1 - - - 1 1
Potassium permanganate KMnO4 100 1 4 1 1 1 - 1 - 1 1
Pyridine C5H5N 100 1 1 4 1 3 4 4 4 1 1
Resorcinol C6H6O2 5 1 4 2 3 1 4 2 - 1 2
Silver nitrate AgNO3 100 1 1 1 1 1 1 1 1 1 4
Sodium bisulfite NaHSO3 10 1 1 2 4 1 - - - 1 1
Sodium carbonate Na2CO3 10 1 1 1 1 1 - - - 1 3
Sodium chloride NaCl 30 1 1 1 1 1 1 1 1 1 3
Sodium hydroxide NaOH 30 1 1 4 1 1 1 1 1 1 4
Sodium hydroxide NaOH 50 1 1 4 1 1 1 1 - 1 4
Sodium sulfate Na2SO4 10 1 1 1 1 1 1 1 1 1 1
Spirits C2H6O 96 1 1 1 1 1 1 1 3 1 1
Styrene C8H8 100 4 1 4 1 3 - 4 4 1 1
Sulfuric acid H2SO4 6 1 4 1 4 1 1 1 - 1 3
Sulfuric acid H2SO4 fuming 4 4 4 4 4 4 4 4 1 3
Tallow — 100 1 1 1 1 1 - 1 1 1 1
Tetrahydrofuran (THF) C4H8O 100 3 1 4 1 3 4 4 4 1 1
Tetrahydronaphthalene C10H12 100 3 1 4 1 4 4 4 4 1 1
Thionyl chloride Cl2SO 100 4 4 4 2 4 4 4 4 1 3
Transformer oil — 100 1 1 3 3 1 1 1 - 1 1
Trichloroethane C2H3Cl3 100 3 3 4 2 4 4 4 4 1 4
Urea CH4N2O 10 1 1 1 1 1 - - - 1 1
Urine — 100 1 1 1 1 1 - 1 1 1 2
Vinegar C2H4O2 10 1 4 1 1 1 1 1 1 1 1
Vinegar C2H4O2 90 1 4 4 4 1 3 1 4 1 1
Wax — 100 - 1 1 1 - - - 1 1
Wines — 100 1 1 1 2 1 1 1 1 1 4
Xylene C8H10 100 3 1 4 1 4 4 4 4 1 1
Zinc chloride SnCl2 10 1 4 2 2 1 - - - 1 4
Inhaltsverzeichnis
EC-Declaration of Conformity
Index
12 Index
Acceleration....................................................................................................... 29, 32, 41, 45
Acceleration curve ..................................................................................32, 35, 37, 45, 52, 81
Accident prevention ............................................................................................................. 15
Acid ............................................................................................................. 18, 61, 86, 87, 88
Activating/deactivating the help function .............................................................................. 51
Adapters .................................................................................................................. 26, 63, 79
Adapters, steel tubes and plastic tubes................................................................................ 76
Alkaline solutions ........................................................................................................... 18, 62
Aluminium accessories ........................................................................................................ 62
Ambient conditions .............................................................................................................. 68
Ambient temperature ........................................................................................................... 68
Autoclaving .......................................................................................................................... 64
Autoclaving table ................................................................................................................. 64
Automatic lid opening function ........................................................................... 32, 37, 50, 52
Automatic program rotation.................................................................................................. 54
Automatical rotor identification ....................................................................................... 31, 44
Blocking a function......................................................................................................... 33, 47
Brake ................................................................................................................................... 45
Brakeless deceleration as of a set speed............................................................................. 49
Brief mains power failure ..................................................................................................... 57
Bucket ............................................................................................................... 16, 26, 62, 63
Buzzer ........................................................................................................................... 34, 51
Calling up the program list ................................................................................................... 30
Calling up the rotor selection list .......................................................................................... 31
Carrier ..................................................................................................................... 26, 63, 64
Centrifugation curves ........................................................................................................... 36
Centrifugation with different tube sizes ................................................................................ 25
Centrifugation with low capacity........................................................................................... 25
Centrifuge cannot be started................................................................................................ 57
Centrifuge decelerates during operation .............................................................................. 57
Centrifuge display .................................................................................................... 24, 27, 41
Centrifuge glass tubes ......................................................................................................... 78
Centrifuge is inclined............................................................................................................ 57
Centrifuge was moved during run ........................................................................................ 57
Centrifuges
- definition .......................................................................................................................... 9
Changes in color.................................................................................................................. 64
Changing of parameters ................................................................................................ 33, 47
Changing the code......................................................................................................... 33, 47
Changing the contrast.......................................................................................................... 52
Changing the scale .............................................................................................................. 46
Chemical and biological safety............................................................................................. 17
Chemical reactions .............................................................................................................. 62
Cleaning agents............................................................................................................. 61, 62
Closing the lid ................................................................................................................ 16, 24
Code.............................................................................................................................. 33, 47
Communication error ........................................................................................................... 59
Condensation ...................................................................................................................... 23
Configuration menu ............................................................................................................. 33
Connected load.................................................................................................................... 68
Connection .......................................................................................................................... 23
Contamination................................................................................................................ 17, 61
Continuous heat resistance.................................................................................................. 64
Version 11/2008, Rev. 1.11 of 01/09/2011 - sb 91
Translation of the original operating manual
4-16
Index
Index
Index
Index
Index
Index
8 Name plate
9 Mains power input
10 Equipotential bonding screw
9
10
1. Before the initial start-up, please ensure that your centrifuge is properly set up and
installed (see chapter 5).
2. Press the mains power switch on the right side of the front to switch on the centrifuge.
3. Open the lid and install the rotor according to the detailed operating instructions (see
chapter 6.2.2).
Manual Mode
• Select a parameter by pressing or
turning the function knob. The selected
field will be inverted.
• Press the function knob in order to
activate the modification mode. "SET" Manual Mode
and the selected area will be inverted.
• Turn the function knob in order to select
• Turn the function knob until the desired a field. The set value of the field starts to
value is displayed. flash.
• Press the function knob to confirm the • Press the function knob. The display
selected value and to quit the starts to flash and the modification mode
modification mode. "SET" and the is active.
selected area will be deactivated.
• Turn the function knob in order to modify
the set value of the selected field.
• Press the function knob again to confirm
the entry and to quit the modification
mode.
7. Parameter values can be modified during the centrifugation run as described under
"Manual Mode" in this document.
8. The centrifugation run can be interrupted at any time by pressing the stop key.