Scribd Logo
Upload

Welcome to Scribd!

  • 417 views

    Ovary Culture

    Uploaded by

    Subha Maheswari
    Ovary culture involves growing ovaries in vitro in a nutrient medium under optimal conditions. This can produce haploid plantlets through fertilization of the egg cell or megaspore via embryogenesis or regeneration from callus tissue. The principle is based on the ovary's ability to regenerate into a fully differentiated plant. Flowers are excised and ovaries removed, then cultured on nutrient medium. This helps study early embryo development, fruit physiology, and the effects of phytohormones. However, the technique has low haploid production rates, requires technical skill, and can only be used in some plant species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd

    Ovary Culture

    Uploaded by

    Subha Maheswari
    417 views3 pages
    Ovary culture involves growing ovaries in vitro in a nutrient medium under optimal conditions. This can produce haploid plantlets through fertilization of the egg cell or megaspore via embryogenesis or regeneration from callus tissue. The principle is based on the ovary's ability to regenerate into a fully differentiated plant. Flowers are excised and ovaries removed, then cultured on nutrient medium. This helps study early embryo development, fruit physiology, and the effects of phytohormones. However, the technique has low haploid production rates, requires technical skill, and can only be used in some plant species.

    Original Description:

    Ovary culture

    Original Title

    Ovary culture

    Copyright

    © © All Rights Reserved

    Available Formats

    DOCX, PDF, TXT or read online from Scribd

    Did you find this document useful?

    Is this content inappropriate?

    Ovary culture involves growing ovaries in vitro in a nutrient medium under optimal conditions. This can produce haploid plantlets through fertilization of the egg cell or megaspore via embryogenesis or regeneration from callus tissue. The principle is based on the ovary's ability to regenerate into a fully differentiated plant. Flowers are excised and ovaries removed, then cultured on nutrient medium. This helps study early embryo development, fruit physiology, and the effects of phytohormones. However, the technique has low haploid production rates, requires technical skill, and can only be used in some plant species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    417 views3 pages

    Ovary Culture

    Uploaded by

    Subha Maheswari
    Ovary culture involves growing ovaries in vitro in a nutrient medium under optimal conditions. This can produce haploid plantlets through fertilization of the egg cell or megaspore via embryogenesis or regeneration from callus tissue. The principle is based on the ovary's ability to regenerate into a fully differentiated plant. Flowers are excised and ovaries removed, then cultured on nutrient medium. This helps study early embryo development, fruit physiology, and the effects of phytohormones. However, the technique has low haploid production rates, requires technical skill, and can only be used in some plant species.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    You are on page 1of 3

    Ovary culture

    Ovary: An ovary is a part of a flower which is the female reproductive part and also refers as
    “Gynoecium”. 

     Ovary culture can define as the in-vitro culturing of ovaries in an aseptic


    condition from the pollinated or un-pollinated flowers, in an appropriate nutrient
    medium and under optimal conditions.
     Ovary culture gives rise to haploid plantlets by the fertilization of egg-cell or
    megaspore either through embryogenesis or regeneration from callus.

    Principle - The principle of gynogenesis is based on the Regeneration principle,


    where an ovary can regenerate into a fully differentiated plant. In the ovary culture, the
    flowers are excised from the plant either in pollinated or non-pollinated stage, and from
    that pistil containing ovary is removed. The ovary comprises of ovule which is a female
    reproductive part of a plant by the growth on nutrient medium and under controlled
    conditions.

    Protocol - Gynogenesis includes the following steps:


    1. First, collect the open flowers from a healthy plant, in a sterilized zip-lock bag.
    2. Wash the flowers with the distilled water.
    3. Then, dip the flowers into the 5% of Teepol solution for 10 minutes.
    4. Again, wash with the flowers with the distilled water.
    5. Wash the flowers again with the distilled water.
    6. Bring the flowers to the laminar airflow chamber.
    7. Then subject the flowers for surface-sterilization by immersing in a 5% of
    sodium hypochlorite solution for 5-7minutes. And, then wash it with the distilled
    water.
    8. To a sterile Petri-plate, transfer the surface sterilized flower and by using sharp
    scalpel dissect out the calyx, petal, anther filaments etc. to separate the ovary.
    9. Then, the ovary can culture either through induction or regeneration process.
    In induction, the ovaries float over the liquid medium. And, in regeneration
    culture the ovaries on the solid nutrient medium.
    10. Incubate the cultures for 16hours at 25֯ C. For the regeneration of ovary, keep the
    culture plates in a daylight regime by using a fluorescent lamp. Place the culture
    tubes in a dark for the induction process.
    11. After 2 weeks, haploid plantlets grow either through embryogenesis or
    through plant regeneration from callus.
    Importance

    Ovary culture helps in the study of the early stages of embryo development.
    Gynogenesis also helps in the study of fruit development and its physiology including
    maturation.
    From the culture of the un-pollinated pistil, the effect of phytohormones on the
    parthenocarpic fruit can be studied. Role of floral organs can be studied which plays a
    significant role in fruit development.
    Gynogenesis also helps in inducing Polyembryony where instead of single plantlet,
    many shoots also develop. Ovary culture also explains the in-vitro
    pollination and seed formation method.
    The culture of an ovary from the apomictic plant can help us to understand the
    stimulus provided by pollination. As in apomistic plant, there is no fertilization, the only
    pollination occurs which alone stimulates the ovary growth and seed development.
    Ovary culture also helps to understand the hybridization process to obtain hybrid
    varieties of seedlings by crossing over of interspecific and intergenic species.
    Limitations

    The frequency of haploid production is very low in Ovary culture as only 1-5% of the


    responding ovaries are there. This technique requires high technical skills and
    management. In some case, besides a haploid plant, some different traits may develop.
    Gynogenesis can be employed only in limited species, for example, wheat, rice,
    maize, sugar beet etc.

    You might also like