MEK7222 Service

MEK- 7222J SERVICE MANUAL
MEK- 7222K
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HEMATOLOGY ANALYZER
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MEK-7222
0634-001896A
Model: MEK-7222J/K
Manual code no.: 0634-001896A
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International Div., Sales Promotion Section, Nihon Kohden Corp., 1-31-4, Nishiochiai Shinjuku-ku, Tokyo
161-8560, Japan
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CONTENTS
Contents
EMC Related Caution ............................................................................................................... i
Conventions Used in this Manual and Instrument .................................................................. iii
Warnings, Cautions and Notes ..................................................................................... iii
Explanations of the Symbols in this Manual and Instrument ........................................ iv
On panel ............................................................................................................. iv
On screen and recorded data .............................................................................. v
Section 1 General .................................................................................. 1C.1

Introduction .......................................................................................................................... 1.1
Service Policy ...................................................................................................................... 1.2
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Specifications ...................................................................................................................... 1.3
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Measured Parameters, Ranges and Reproducibility to Specimen from Venous
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Blood ................................................................................................................ 1.3
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Detection Method ............................................................................................. 1.3
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Standardization Analysis Method ..................................................................... 1.3
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Interference Substances .................................................................................. 1.4
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eennpp Display ............................................................................................................. 1.6
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Data Storage .................................................................................................... 1.6
Environmental Conditions ................................................................................. 1.6
nng Power Requirements ........................................................................................ 1.6

Dimensions and Weight .................................................................................... 1.7
Electromagnetic Compatibility .......................................................................... 1.7
Safety .............................................................................................................. 1.7
Panel Descriptions ................................................................................................................ 1.8
Front Panel ................................................................................................................. 1.8
Right Side Panel ......................................................................................................... 1.9
Rear Panel ............................................................................................................... 1.10
Inside Panels ........................................................................................................... 1.11
Composition ....................................................................................................................... 1.12
Standard Composition .............................................................................................. 1.12
Options ..................................................................................................................... 1.14
Section 2 Troubleshooting ................................................................... 2C.1

General ................................................................................................................................. 2.1
Error Messages .................................................................................................................... 2.2
System Error Messages ....................................................................................................... 2.5
Inaccurate Counting and Other Problems ............................................................................. 2.6
Section3 Board/Unit Description ........................................................ 3C.1

CBC Measuring Unit ............................................................................................................. 3.1
Service Manual MEK-7222 C.1

CONTENTS
Diluter Unit ........................................................................................................................... 3.2

Pump Unit ............................................................................................................................ 3.2
Triple Pump Unit ................................................................................................................... 3.3
Sampler Unit ........................................................................................................................ 3.3
Power Supply Unit and POWER Board ................................................................................. 3.4
Inlet/Outlet Unit .................................................................................................................... 3.4
Sheath Unit .......................................................................................................................... 3.5
Front Panel Unit .................................................................................................................... 3.5
Optional Printer Unit WA-720VK ........................................................................................... 3.6
Rinse Unit and Switch Assembly ......................................................................................... 3.6
Laser Optical Unit ................................................................................................................. 3.7
Flow Cell Adjuster ................................................................................................................ 3.7
AMP CONTROL Board ......................................................................................................... 3.7
DRIVER Board ..................................................................................................................... 3.8
Optional Cap Pierce Unit MS-721V ...................................................................................... 3.8
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Section4
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Disassembly and Assembly ...............................................
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Before You Begin .................................................................................................................. 4.1
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Warnings and Cautions ............................................................................................... 4.1
Required Tools ............................................................................................................ 4.1
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Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the
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Instrument .................................................................................................................. 4.2
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Board and Unit Location ....................................................................................................... 4.3
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Right Side .................................................................................................................. 4.3
lest Side ..................................................................................................................... 4.3
nng Upper Part of Right Side ............................................................................................. 4.4

Lower Part of Right Side ............................................................................................. 4.4
Front View of Chassis ................................................................................................. 4.4
Draining the Hematology Analyzer ....................................................................................... 4.5
Opening the Front Cover ....................................................................................................... 4.7
Removing the Top Cover ....................................................................................................... 4.8
Replacing the Boot ROM ...................................................................................................... 4.9
Removing the Rear Cover Including the Power Supply Unit ............................................... 4.11
Removing the CBC Measuring Unit .................................................................................... 4.12
Removing the Diluter Unit ................................................................................................... 4.13
Removing the Sampler Unit ................................................................................................ 4.15
Removing the Pump Unit .................................................................................................... 4.16
Removing the Triple Pump Unit .......................................................................................... 4.18
Removing the WA-720VK Printer Unit (Option) ................................................................... 4.19
Removing the Front Panel Unit ........................................................................................... 4.20
Removing the Inlet/Outlet Unit ........................................................................................... 4.22
Removing the Flow Cell Adjuster ........................................................................................ 4.24
Removing the Laser Optical Unit ........................................................................................ 4.26
Removing the Sheath Unit .................................................................................................. 4.28
Removing the Rinse Unit and Switch Assembly ................................................................. 4.31
Replacing the AMP CONTROL Board ................................................................................ 4.32
Replacing the DRIVER Board ............................................................................................. 4.33
Replacing the VOLUME Board, KEY DISPLAY Board and LCD Unit .................................. 4.34
Replacing the MS-721V Cap Pierce Unit (Option) .............................................................. 4.36
C.2 Service Manual MEK-7222

CONTENTS
Section 5 Adjustment ............................................................................ 5C.1

Adjusting HGB Sensor Output Voltage ................................................................................. 5.1
Adjusting Upper and Lower Sensor Output Voltage of the Manometer .................................. 5.2
Compensating manometer Volume ....................................................................................... 5.3
Adjusting Gain for WBC 5 Part Differential Measurement ..................................................... 5.4
Counting the Polymer Microsphere Suspensions and
Adjusting the Flow Cell Position ................................................................................. 5.5
Adjusting Gain for WBC 5 Part Differential Measurement Roughly
by Polymer Microsphere Suspensions ....................................................................... 5.9
Adjusting Gain for WBC 5 Part Differential Measurement Finely
by Human Blood Sample .......................................................................................... 5.10
Editing the Peak List ................................................................................................ 5.13
Manually Adjusting the Gain .................................................................................... 5.15
Compensating Dilution Ratio .............................................................................................. 5.15
Calibrating Touch Screen .................................................................................................... 5.16
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Adjusting Liquid Sensor Output Voltages ............................................................................ 5.17
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Adjusting Contrast of LCD .................................................................................................. 5.18
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Section 6 Maintenance ......................................................................... 6C.1
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To be Replaced Periodically .................................................................................................. 6.1
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Periodic Maintenance Check Procedure ............................................................................... 6.1
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Preparation ................................................................................................................. 6.1
eennpp Appearance ................................................................................................................ 6.1
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Safety ........................................................................................................................ 6.1
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Reagents .................................................................................................................... 6.1
Cleaning/Replacing ..................................................................................................... 6.2
SENSOR MONITOR screen ....................................................................................... 6.3
CIRCUIT CHECK Screen ........................................................................................... 6.3
Background noise ...................................................................................................... 6.4
Measuring the Polymer Microsphere Suspensions ..................................................... 6.4
Measuring the MEK-5DN Hematorogy Control ........................................................... 6.4
Current Calibration Coefficients .................................................................................. 6.4
New Calibration Coeffisients ...................................................................................... 6.4
Software Version ........................................................................................................ 6.5
Checking the Operations ............................................................................................ 6.5
Built-in Printer Unit Operation (When the printer is installed) ...................................... 6.5
External Printer Unit Operation (When the external printer is connected) ................... 6.6
Bar Code Reader Operation (WHen the bar code reader is installed) .......................... 6.6
Others ........................................................................................................................ 6.6
Displaying Operation History Screen .................................................................................... 6.7
General ....................................................................................................................... 6.7
Displaying the OPERATION HISTORY Screen ........................................................... 6.7
Checking, Cleaning or Replacing Filters ............................................................................... 6.8
Checking and Cleaning the Sub Baths, Measurement Baths, and Sample Cup ................. 6.10
Checking and Cleaning the Rinse Unit and Sampling Nozzles ........................................... 6.12
Checking and Replacing the Pump Tubes ........................................................................... 6.14
Cleaning the Aperture Caps ............................................................................................... 6.16
Checking and Replacing the Sampling Nozzles ................................................................. 6.19

CONTENTS
Checking and Cleaning the Cap Pierce Rinse Unit, Sampling Nozzle and
Cap Pierce Needle (Optional MS-721V Cap Pierce Unit) .................................................... 6.21
Checking and Replacing the Cap Pierce Needle (Optional MS-721V Cap Pierce Unit) ....... 6.23
Calibrating Touch Screen .................................................................................................... 6.25
Maintenance Check Sheet ................................................................................................. 6.26
Section 7 Test Point, Variable Resistor, LED and Switch on Board. 7C.1
PRE AMP board ................................................................................................................... 7.1
HGB AMP board .................................................................................................................. 7.2
MANOMETER board ............................................................................................................ 7.2
MV CONNECTION board ..................................................................................................... 7.3
4WAY LIQUID SENSOR board ............................................................................................. 7.4
SHEATH board ..................................................................................................................... 7.5
TRIPLE PUMP board ........................................................................................................... 7.6
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DILUTER DRIVER board ...................................................................................................... 7.7
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SAMPLER-X board .............................................................................................................. 7.8
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SAMPLER-Z board ............................................................................................................... 7.8
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KEY DISPLAY board ............................................................................................................ 7.9
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VOLUME board .................................................................................................................... 7.9
POWER board .................................................................................................................... 7.10
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AMP CONTROL board ....................................................................................................... 7.12
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DRIVER board .................................................................................................................... 7.14
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Section 8 Demonstration
nng Introduction .......................................................................................................................... 8.1
Demonstration Outline ................................................................................................ 8.1
Required Items for Demonstration .............................................................................. 8.2
Preparing the Hematology Analyzer ..................................................................................... 8.4
Installation Flowchart ................................................................................................. 8.4
Connecting Tubes ....................................................................................................... 8.4
Connecting the Power Cord and the Grounding the Hematology Analyzer .................. 8.6
Turning the Laser Switch On ...................................................................................... 8.6
Turning On the Power ................................................................................................. 8.7
Setting the Date & Time and Cleaning the Hematology Analyzer ......................................... 8.8
Checking the Date and Time Settings ........................................................................ 8.8
Cleaning the Fluid Path in the Hematology Analyzer .................................................. 8.8
Adjusting Gain and Measuring Background Noise ................................................................ 8.9
Flowchart ................................................................................................................... 8.9
Reference: Principle of Differentiating WBC ............................................................... 8.9
Counting the Polymer Microsphere Suspensions ..................................................... 8.10
Adjusting the Flow Cell Unit Position ........................................................................ 8.12
Adjusting Gain (FS and FL) for WBC 5 Part Differential Parameters in Rough Mode
Using Polymer Microsphere Suspensions ................................................................ 8.15
Measuring Background Noise ................................................................................... 8.16
Adjusting Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous
Blood Samples ......................................................................................................... 8.17
Checking the Gain Adjustment ................................................................................. 8.20

CONTENTS
Calibration .......................................................................................................................... 8.21

Procedure Flowchart ................................................................................................ 8.21
Calibration for CBC Paramters with Calibrator .......................................................... 8.21
Calibration for WBC 5 Part Differential Parameters ................................................... 8.26
Checking Data .................................................................................................................... 8.27
Checking Data with MEK-5D Hematology Control .................................................... 8.27
Checking Data with Human Blood Samples ............................................................. 8.28
Interference Substances .................................................................................................... 8.29
Storing and Transporting the Hematology Analyzer ............................................................ 8.32
MEK-7222J/K Hematology Analyzer Installation Check Sheet ........................................... 8.34
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CONTENTS
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EMC RELATED CAUTION
This equipment and/or system complies with the International Standard EN61326-1 for electromagnetic
compatibility for electrical equipment and/or system for measurement, control and laboratory use.
However, an electromagnetic environment that exceeds the limits or levels stipulated in the EN61326-1,
can cause harmful interference to the equipment and/or system or cause the equipment and/or system to
fail to perform its intended function or degrade its intended performance. Therefore, during the
operation of the equipment and/or system, if there is any undesired deviation from its intended
operational performance, you must avoid, identify and resolve the adverse electromagnetic effect before
continuing to use the equipment and/or system.
The following describes some common interference sources and remedial actions:
1. Strong electromagnetic interference from a nearby emitter source such as an authorized radio station
or cellular phone:
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Install the equipment and/or system at another location if it is interfered with by an emitter source
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such as an authorized radio station. Keep the emitter source such as cellular phone away from the
equipment and/or system.
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and/or system:
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2. Radio-frequency interference from other equipment through the AC power supply of the equipment
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Identify the cause of this interference and if possible remove this interference source. If this is not
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possible, use a different power supply.
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3. Effect of direct or indirect electrostatic discharge:
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Make sure all users and patients in contact with the equipment and/or system are free from direct or
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indirect electrostatic energy before using it. A humid room can help lessen this problem.
4. Electromagnetic interference with any radio wave receiver such as radio or television:
If the equipment and/or system interferes with any radio wave receiver, locate the equipment and/or
system as far as possible from the radio wave receiver.
If the above suggested remedial actions do not solve the problem, consult your Nihon Kohden
Corporation subsidiary or distributor for additional suggestions.
This equipment complies with International Standard EN55011 (1999) Group 1, Class B. Class B
EQUIPMENT is equipment suitable for use in domestic establishments and in establishments directly
connected to a low voltage power supply network which supplies buildings used for domestic purposes.
Service Manual MEK-7222 i

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ii Service Manual MEK-7222

Conventions Used in this Manual and Instrument
Warnings, Cautions and Notes
Warnings, cautions and notes are used in this manual to alert or signal the reader to specific information.
WARNING
A warning alerts the user to the possible injury or death associated with the use or misuse of the
instrument.
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A caution alerts the user to possible injury or problems with the instrument associated with its use or
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misuse such as instrument malfunction, instrument failure, damage to the instrument, or damage to other
property.
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A note provides specific information, in the form of recommendations, prerequirements, alternative
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methods or supplemental information.
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Service Manual MEK-7222 iii

Explanations of the Symbols in this Manual and Instrument
The following symbols found in this manual/instrument bear the respective descriptions as given.
On panel
Symbol Description Symbol Description
AC power off ISOTONAC•3

(Disconnection from the mains) (diluent)
AC power on CLEANAC
(Connection to the mains) (detergent)
CLEANAC•3
Main power lamp
(detergent)
HEMOLYNAC•3*
“On” only for part of the equipment
(hemolysing reagent)
HEMOLYNAC•3N*
“Off” only for part of the equipment
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Laser ON
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Reset
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Clean
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Capillary blood mode Alternating current
nng Count Equipotential terminal
Dispense Printer socket
Auto print 1 Serial port 1
Bar code reader socket

Feed 2 (Serial port 2)
Print Biohazard
Inlet Year of manufacture
Outlet Serial number
Screen brightness
* When using the HEMOLYNAC•3N hemolysing reagent, attach the HEMO3N label over the HEMO3 label.
iv Service Manual MEK-7222

On screen and recorded data
Symbol Description Symbol Description

Beside WBC or RBC measured
value: Sample error Beside HGB measured value: HGB
Beside HGB measured value: Dirty circuit error
measurement baths
Beside WBC measured value: Poor
hemolyzation
Beside HGB measured value: HGB Beside WBC or PLT measured
voltage adjustment error C value: Platelet coagulation
Beside MCHC measured value:
Abnormal MCHC
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Service Manual MEK-7222 v

Section 1 General
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Introduction ......................................................................................................................... 1.1
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Service Policy ..................................................................................................................... 1.2
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Specifications ..................................................................................................................... 1.3
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Measured Parameters, Ranges and Reproducibility to Specimen from Venous
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Blood ............................................................................................................... 1.3
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Detection Method ............................................................................................ 1.3
Standardization Analysis Method .................................................................... 1.3
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eennpp Dilution Ratio ................................................................................................... 1.6
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Counting Time ................................................................................................. 1.6
Display ............................................................................................................ 1.6
nng Data Storage ................................................................................................... 1.6
Environmental Conditions ................................................................................ 1.6
Power Requirements ....................................................................................... 1.6
Dimensions and Weight ................................................................................... 1.7
Electromagnetic Compatibility ......................................................................... 1.7
Safety ............................................................................................................. 1.7
Panel Descriptions ............................................................................................................... 1.8
Front Panel ................................................................................................................ 1.8
Right Side Panel ........................................................................................................ 1.9
Rear Panel .............................................................................................................. 1.10
Inside Panels .......................................................................................................... 1.11
Composition ...................................................................................................................... 1.12
Standard Composition ............................................................................................. 1.12
Options .................................................................................................................... 1.14
Service Manual MEK-7222 1C.1

1. GENERAL
Introduction
CAUTION
To maintain the instrument in normal condition, the user must perform
the periodic maintenance. Refer to “Maintenance” of the operator’s
manual.
This service manual provides useful information to qualified service personnel to

understand, troubleshoot, service, maintain and repair the MEK-7222 Hematology
Analyzer (referred to as “the instrument” in this service manual).
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The maintenance must be periodically performed because the instrument has fluid
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paths and precision parts. Accordingly, the user is responsible for performing the
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periodic maintenance. The “Maintenance” section in this service manual
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describes the maintenance that should be performed by qualified service
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personnel. The “Maintenance” section in the operator’s manual describes the
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maintenance that can be performed by the user.
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eennpp If the instrument has a problem and there has been no periodic
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nng the fluid paths or replacing a consumable with a new one.
The information in the operator’s manual is primarily for the user. However, it is
important for service personnel to thoroughly read the operator’s manual and
service manual before starting to troubleshoot, service, maintain or repair this
instrument. This is because service personnel needs to understand the operation
of the instrument in order to effectively use the information in the service manual.
Service Manual MEK-7222 1.1

1. GENERAL
Service Policy
CAUTION
• Be careful not to directly touch any place where blood is or may
spread to.
• Wear rubber gloves to protect yourself from infection before doing
maintenance.
Nihon Kohden Corporation’s basic policy for technical service is to replace faulty
units, printed circuit boards or parts. We do not support component-level repair of
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boards and units outside the factory.
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• When ordering parts or accessories from your nearest Nihon Kohden
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Corporation’s distributor, please quote the NK code number and part
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name which is listed in this service manual, and the name or model
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of the unit in which the required part is located. This will help us to
eennppromptly attend to your needs.
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• Always use parts and accessories recommended or supplied by
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1.2 Service Manual MEK-7222

1. GENERAL
Specifications
Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood

Specifications except WBC population were determined using hematology control blood (MEK-3DN), counted 10
times consecutively.
Reproducibility to Specimen from Venous
Measured Parameters Measuring Range Blood
(CV: Coefficient of Variation)
WBC: White blood cell count 0 to 300 × 103/µL within 2.0%CV (4.0 to 9.0 × 103/µL)
within 5.0%CV (WBC: 4.0 to 9.0 × 103/µL,
NE%: Neutrophil percent 0 to 99.9%
NE%: 40 to 70%)
LY%: Lymphocyte percent 0 to 99.9%
LY%: 20 to 45%)
MO%: Monocyte percent 0 to 99.9%
MO%: 2 to 10%)
EO%: Eosinophil percent
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0 to 99.9%
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within CV30.0% (>2%) or average value ±1% (0 to
BA%: Basophil percent
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2%) (WBC: 4.0 to 9.0 × 103/µL, BA%: 0 to 3%)
NE: Neutrophil count
LY: Lymphocyte count
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0 to 99.9 × 103/µL
0 to 99.9 × 103/µL
MO: Monocyte count
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EO: Eosinophil count
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BA: Basophil count
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RBC: Red blood cell count
0 to 99.9 × 103/µL
0 to 14.9 × 106/µL within 1.5%CV (5.0 × 106/µL)
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HGB: Hemoglobin concentration 0 to 29.9 g/dL within 1.5%CV (16 g/dL)
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HCT: Hematocrit
MCV: Mean cell volume
0 to 99.9%
20 to 199 fL within 1.0%CV (70 to 120 fL)
MCH: Mean cell hemoglobin 10 to 50 pg
MCHC: Mean cell hemoglobin concentration 10 to 50 g/dL
RDW: Red blood cell distribution width 0 to 50%
PLT: Platelet count 0 to 1490 × 103/µL within 4.0%CV (3.0 × 103/µL)
PCT: Platelet crit 0 to 2.9%
MPV: Mean platelet volume 0 to 20.0 fL
PDW: Platelet distribution width 0 to 50%
Detection Method
Blood cell count: Electrical resistance detection
Hemoglobin: Cyanmethemoglobin optical detection
Hematocrit: Histogram calculation
WBC population: Light scatter by laser
Platelet crit: Histogram calculation
RBC distribution width: Histogram calculation
Platelet distribution width: Histogram calculation
Standardization Analysis Method

WBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood cell counters.
Clin Lab Haematol, 10:203-212, 1988
RBC: ICSH1988 ICSH: The assignment of values to fresh blood used for calibrating automated blood cell counters.
Clin Lab Haematol, 10:203-212, 1988
1. GENERAL
HGB: NCCLS H15-A2 H15-A2: Reference and Selected Procedures for the Quantitative Determination of Hemoglobin
in Blood Second Edition; Approved Standard (1994)
HCT: NCCLS H7-A2 H7-A2: Procedure for Determining Packed Cell Volume by the Microhematocrit Method Second
Edition; Approved Standard (1993)
PLT: Brecher & Cronkite
Interference Substances
WBC: Unlysed red cells
In some rare occasions, the RBC in the blood sample may not completely lyse and these non-lysed
RBC may be detected as WBC and cause increase in WBC count.
Multiple myeloma
The precipitation of proteins in multiple myeloma patients may increase the WBC count.
Leukemia
WBC is fragile in leukemia patient and WBC may be destroyed during measurement. These WBC
fragments may also interfere with WBC differential measurement.
Chemotherapy
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Cytotoxic and immunosuppressive drugs cause low WBC count.
Cryoglobulins
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Cryoglobulin may be increased in patients who are or have myeloma, cancer, leukemia,
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macroglobulinemia, lymphoproliferative disorders, metastatic tumors, autoimmune disorders,
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infections, anerurism, pregnant, thromboembolic phenomena, diabetes, etc, which cause increase in
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WBC, RBC or PLT counts and HGB concentration. In such cases, warm the blood sample to 37°C in a
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water bath for 30 minutes and measure the sample immediately.
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RBC: Leukemia
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An increase in WBC in leukemia patient causes increase in RBC.
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Agglutinated RBC
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Agglutinated RBC may decrease RBC count. This can be checked by abnormal MCH and MCHC
values and examination of the stained blood film.
Cold agglutinins
IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT
counts and increase MCV.
HGB: Turbidity of the blood sample
Any physiologic and/or therapeutic factors may increase HGB. In such a case, determine the cause of
turbidity and follow the appropriate method below.
1. Increased WBC
An extreme increase in WBC will cause excessive light scatter. In these cases, measure manually.
Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer.
2. Increased lipids
The blood sample may be milky when there is excessive lipid. This may occur with
hyperlipidemia, hyperproteinemia and hyperbilirubinemia. Accurate HGB measurement can be
achieved by manual methods and a plasma blank.
3. Increased turbidity
When RBC are resistant to lysing, turbidity may increase causing increase in HGB. Observe if
MCH and MCHC values are abnormal. HGB result affects MCH and MCHC result.
4. Fetal bloods
The mixing of fetal and maternal blood may increase HGB value.
HCT: Agglutinated RBC
RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values
are abnormal. In such a case, measure manually.

1. GENERAL
MCV: Agglutinated RBC

RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values
are abnormal. In such a case, measure manually.
Excessive number of large PLT
Excessive number of large PLT and/or excessively high WBC may affect the MCV value. Check by
careful examination of the stained blood film.
MCH: MCH is determined from HGB and RBC values. Therefore, the limitations for HGB and RBC also
affect MCH value.
MCHC: MCHC is determined from HGB and HCT values. Therefore, the limitations for HGB and HCT also
affect MCHC value.
RDW: RDW is determined from RBC value. Therefore, the limitations for RBC also affect RDW value.
Agglutinated RBC
Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked by abnormal
MCH and MCHC values and examination of the stained blood film.
Nutritional deficiency or blood transfusion
PLT: Very small fragments

.ccoom
Iron and/or cobalamin and/or folate deficiency may increase RDW.
m
aaiill.
Very small RBC, RBC fragments and WBC fragments may be the cause in increased PLT count.
Agglutinated RBC
ggmm
eed@@
PLT may be trapped in the agglutinated RBC resulting in decrease in PLT. This can be checked by
d
abnormal MCH and MCHC values and examination of the stained blood film.
Very large PLT
n
n g
g m
m
u o
o
Large PLT may exceed the PLT threshold and may not be counted which results in low PLT count.
hh u
eennpp
Chemotherapy
g u
uy
Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT
y
count. In such a case, measure manually.
nng Hemolysis
Hemolyzed specimens contain red cell stroma which may increase PLT count.
Anticoagulated blood
Blood anticoagulated with acid-citrate-dextrose may have clumped PLT which may cause decrease in
PLT count.
Agglutinated PLT
Clumped PLT may decrease PLT count and/or increase WBC count. For such sample, collect the
sample in sodium citrate anticoagulant and measure only PLT. The PLT result must be corrected for the
sodium citrate dilution effect.
MPV: Very large PLT
Large PLT may exceed the PLT threshold and not be counted which results in low MPV.
Very small fragments
Very small RBC, RBC fragments and WBC fragments may interfere with MPV measurement.
Agglutinated RBC
PLT may be trapped in the agglutinated RBC resulting erroneous MPV. This can be checked by
Chemotherapy
Cytotoxic and immunosuppressive drugs may affect MPV. In such a case, measure manually.
NOTE
Blood samples collected in EDTA does not maintain stable MPV because platelets swell
depending on the interval after collection and storage temperature.

1. GENERAL
WBC 5 part differential parameters are derived from the WBC count, therefore, the limitations for WBC also affect these
parameters.
LY and LY%: Erythroblasts, certain parasites and RBC that are resistant to lysis may interfere with an accurate LY
count.
MO and MO%: Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils may interfere
with an accurate MO count.
NE and NE%: Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may
interfere with an accurate NE count and NE%.
EO and EO%: Abnormal granules may interfere with an accurate EO count.
BA and BA%: Immature cell, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere with
an accurate BA count and BA%.
Dilution Ratio
• Venous blood
Sample volume: 55 µL (for 22 parameters)/30 µL (for WBC, RBC, HGB, HCT, MCV, MCH, MCHC and PLT)
WBC/HGB:
RBC/PLT:
200:1
40,000:1
.ccoomm
• Capillary blood
aaiill.
Sample volume: 10 µL 20 µL
ggmm
WBC/HGB:
RBC/PLT:
1200:1
240,000:1
600:1
ed
e
120,000:1
d@@
n
n g
g m
m
Counting Time
hhuuo
o
eennpp
Within 70 s/sample (from measurement start to data display)
Display
g u
uyy
Display:
Resolution:
nng 5.5 inch LCD with backlight and touch screen keys
320 × 240 dots
Screen size: approx. 86 × 115 mm
Display contents: Numerical data, scattergrams, histograms, measuring conditions, alarm message and other
messages, touch screen keys
Data Storage
Numerical data for all counted parameters for up to 400 samples and histograms and scattergrams for up to 50 samples
Environmental Conditions
Storage temperature: −20 to 60°C
Operating temperature: 15 to 30°C
Storage humidity: 10 to 95% (Non-condensing)
Operating humidity: 30 to 85% (Non-condensing)
Storage atmospheric pressure: 70 to 106 kPa
Operating atmospheric pressure: 70 to 106 kPa
Power Requirements
Power requirements: MEK-7222J: 110, 117 or 127 V ±10% AC, 50/60 Hz
MEK-7222K: 220, 230 or 240 V ±10% AC, 50/60 Hz
Power consumption: 250 VA

1. GENERAL
Dimensions and Weight

Dimensions: 382 W × 485 D × 520 H (mm)
Net weight: approx. 38 kg
Electromagnetic Compatibility
IEC 61326-1 (1997) Amendment 1 (1998) Amendment 2 (2000)
EN 61326-1 (1997) Amendment 1 (1998)
CISPR11 (1997), Group 1, Class B

EN 55011 (1998) Amendment 1 (1999), Group 1, Class B
The power supply short interruption test is performed through the transformer which has three times or more as large
capacity as the instrument.
Safety
Safety standards: IEC 61010-1 2nd Edition (2001)
EN 61010-1 2nd Edition (2001)
.ccoomm
aaiill.
Laser: IEC 60825-1 (1993) Amendment 1 (1997), Class 1
g mm
EN 60825-1 (1994) Amendment 11 (1996)
g
ed
ed@@
According to the type of protection against electrical shock: CLASS I EQUIPMENT
According to the degree of protection against harmful ingress of water: IPX0 (Ordinary EQUIPMENT)
n g
g m
m
According to the degree of safety of application in the presence of a FLAMMABLE ANAESTHETIC MIXTURE WITH AIR,
n
u o
o
OR WITH OXYGEN OR NITROUS OXIDE: EQUIPMENT not suitable for use in the presence of FLAMMABLE
hh u
eennpp ANAESTHETIC MIXTURE WITH AIR, OR WITH OXYGEN OR NITROUS
g u
uyy
OXIDE
According to the mode of operation: CONTINUOUS OPERATION
nng
EQUIPMENT types (classification): Indoor stationary EQUIPMENT
Pollution Degree: 2 EQUIPMENT
Requirements for marking of IN VITRO DIAGNOSTIC instruments: EN1658 (1996)

1. GENERAL
Panel Descriptions
Front Panel
1 14
15
2 16
3 17
4 18
5
6
19
7
8
9
.ccoomm 20
10
aaiill.
11
12
ggmm
13
ed
ed@@
No. Name
n
n g
g m
m Description
1 LCD display
u o
o
Displays various messages, measured data and touch screen keys.
hh u
2
3
Power lamp
Main power lamp
eennp
Lights when the main power switch on the rear panel and power key on the front panel are turned on.
p
Lights when the main power switch on the rear panel is tuned on.
4 Laser lamp
g u
uyy Lights when the laser switch on the right side panel is turned on.
5 Power key nng Turns the hematology analyzer power on or off when the main power switch on the rear panel is
turned on. When the power is turned on, priming and self-check are automatically performed, and the
READY screen appears.
Stops operation when pressed during operation. Returns to the READY screen when pressed while
6 Reset key
changing settings. Use this key only when an error occurs.
Cleans the fluid path, aperture and manometer with detergent. Automatically primes after cleaning the
7 Clean key fluid path. Press this key when clogging occurs, the manometer becomes dirty or bubbles occur in the
manometer.
Auto print mode
8 Lights when automatic printing mode is selected (optional).
lamp
9 Auto print key Switches the printing mode between automatic and manual for the WA-720VK printer unit (optional).
10 Feed key Feeds paper of the WA-720VK printer unit while held down (optional).
11 Print key Prints measured data on the WA-720VK printer unit (optional).
Printer unit
12 Roll printer. Prints out measured data and sample ID no. (optional)
(WA-720VK)
13 Printer door For the recording paper of the WA-720VK printer unit. To open, pull the upper right corner (optional).
Screen brightness
14 Adjusts the screen brightness
control
Capillary blood
15 Lights when in capillary blood mode.
mode lamp
Capillary blood
16 Switches the operation mode to capillary blood mode. The capillary blood mode lamp lights.
mode key
Dispenses diluent (approx. 2 mL) from the sampling nozzle. Available only for the capillary blood
17 Dispense key
mode.
Blinking: Aspirating the sample
Operation indicator
18 Off: Counting the sample
lamps
Lit: Ready for next counting
19 Sampling nozzle Aspirates the sample. Dispenses the diluent when in the capillary blood mode.
20 Count switch Aspirates the sample and starts counting.

1. GENERAL
Right Side Panel
3
4
5
Refer to “Connecting the Tubes” in
Section 2 of the operator’s 6
manual.
7
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy No. Name Description
nng
Turns the laser on or off with the laser key for WBC 5 part
1 Laser switch
differential measurement.
ISO3
2 Inlet for the ISOTONAC•3 diluent.
Diluent inlet
CLN
3 Inlet for the CLEANAC detergent.
Detergent inlet
CLN3
4 Inlet for the CLEANAC•3 detergent.
Detergent inlet
HEMO3/HEMO3N Inlet for the HEMOLYNAC•3 or HEMOLYNAC•3N
5
Hemolysing reagent inlet* hemolysing reagent.
HEMO5
6 Inlet for the HEMOLYNAC•5 hemolysing reagent.
Hemolysing reagent inlet
WASTE Outlet for waste such as used lyse, detergent and aspirated
7
Waste outlet samples.
* When using the HEMOLYNAC•3N reagent, attach the HEMO3N label
provided with the hematology analyzer to cover the HEMO3 label at the
HEMO3 inlet.

1. GENERAL
Rear Panel
Refer to warnings and

cautions in “Connecting an
1 External Instrument to the
Hematology Analyzer” in
2 Section 2 of the operator’s
manual.
.ccoomm
aaiill. 4
ggmm 5
ed
ed@@ 6
n
n g
g m
m 7
hhuuo
o
eennpp
Refer to warnings and cautions in “Connecting the Power
u
uyy
Cord and Grounding the Hematology Analyzer” in Section
g
nng
2 of the operator’s manual.
No. Name Description

Connects to the optional WA-460V card printer, other external
1 Serial port
printer or PC. (Serial port 1)
Connects to the optional handy bar code reader. Can be used as a
serial port. (Serial port 2)
2 Bar code socket Also supplies the power to the bar code reader.
Power supply voltage: 5 VDC (pin 9: 5 V, pin 5: GND)
Rated current: 200 mA
3 Printer socket Connects to an external printer (WA-710V or other).
Supplies the power to the hematology analyzer when it is turned
4 Main power switch
on. Under normal conditions keep this switch turned on.
Contains the time lag fuse. To replace the fuse, contact your
5 Fuse holder
Nihon Kohden distributor.
AC SOURCE Connects the AC power cord to supply AC power to the
6
AC source socket hematology analyzer.
Equipotential ground Connects the ground lead to the equipotential ground terminal on
7
terminal the wall for earth grounding.
CAUTION
• In order to avoid any safety hazard, only connect personal
computers which are approved by IEC 60950 using the information
found in the reference in Section 10.
• Only use the 3-prong power cord for the PC.

1. GENERAL
Inside Panels
CAUTION
• For WBC 5 part differential measurement, the laser beam is used. Do
not open any part labeled “CAUTION”. The laser can cause burns
and blindness.
• Do not remove the caution labels.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
NOTE
• Replace the filter periodically.
• When attaching the filter joint assembly, be careful not to bend or
damage the filter packing at the bottom of the measurement bath.
• When there is a leakage, check that there is no scratch or damage to
the circumference of the filter.

1. GENERAL
Composition
Standard Composition
MEK-7222K
CD-720V Chassis
MEK-7222J
MA-720V Flow Cell Adjuster
MC-720V CBC Measuring Unit
UT-71351 CBC Measuring Board Set
UT-71211 PRE AMP Board
UT-71301
.ccoomm
MANOMETER Board
iill.
UT-71311
aa
HGB AMP Board
ggmm
UT-7133 HGB LED Board
ed
ed@@ UT-71341 MV CONNECTION Board
n
n g
g m
m
hhuuo
o
UT-7137 LED Board
eennpp
g u
uyy
XP-512V 2-way Valve
nng MD-630V
UT-7119
Diluter Unit
DILUTER DRIVER Board
XP-513V 3-way Valve
MJ-720V Inlet/Outlet Unit
UT-7179 4WAY LIQUID SENSOR Board
XP-513V 3-way Valve
MJ-721V Sheath Unit
UT-7169 SHEATH Board
XP-512V 2-way Valve
XP-513V 3-way Valve
XP-513VS 3-way Valve

1. GENERAL
MO-820V Laser Optical Unit
MF-820V Flow Cell Unit
UT-7172 MO Preamp Board Set
UT-7173 MO FS PREAMP Board
UT-7174 MO FL PREAMP Board
UT-7175 MO SD PREAMP Board
UT-7176 MO LD DRIVER Board
MP-520V Pump Unit (For CBC)
MP-520V Pump Unit (For WBC 5 part)
.ccoomm
MP-720V
aaiill. Triple Pump Unit
ggmm
ed
ed@@UT-7170 TRIPLE PUMP Board
n
n g
g m
m XP-503V 3-way Valve
hhuuo
o XP-513V 3-way Valve
eennpp
g u
uyy
XP-513VS 3-way Valve
nng MR-720V
MS-720V
Rinse Unit and Switch Assembly
Sampler Unit
UT-7165 SAMPLER-X Board
UT-7166 SAMPLER-Z Board
XP-513V 3-way Valve
PV-720V Front Panel Unit
UT-7188 Front Panel Board Set
UT-7167 KEY DISPLAY Board
UT-7168 VOLUME Board
SC-720VJ for MEK-7222J

Power Supply Unit
SC-720VK for MEK-7222K
UT-7162 AMP CONTROL Board
UT-7163 DRIVER Board
UT-7164 POWER Board

1. GENERAL
Options WA-720VK Printer unit
UT-7136 Printer Board Set
UT-7123 PRINTER INTERFACE Board
UT-7124 PRINTER KEY Board
MS-721V Cap Pierce Unit
UT-7189 Cap Pierce Board Set
MT-7190 CAP PIERCE -1 Board
MT-7191 CAP PIERCE -2 Board
XP-513V
.ccoom
3-way Valve
m
aaiill.
WA-460V
ggmm Card Printer
ed
ed@@
n
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g m
m
hhuuo
o
eennpp
g u
uyy
nng

Section 2 Troubleshooting
.cc omm
General ................................................................................................................................ 2.1
o
aa ill.
Error Messages ................................................................................................................... 2.2
i
System Error Messages ...................................................................................................... 2.5
ggmm
Inaccurate Counting and Other Problems ............................................................................ 2.6
ed
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n
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hhuuo
o
eennpp
g u
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nng

2. TROUBLESHOOTING
General
If a problem occurs, the built-in error detection function displays an error message
with an alarm sound. The tables in the next sections show the types of error
message, their causes and their countermeasures. Use them when an error message
occurs.
There are two types of error messages.

• Error message when turning the power on
• Error message related to measurement
CAUTION
When an alarm occurs, the acquired data may not be correct, especially
.ccoomm
when the “!” or “sample error” message appears. Do not use the
aaiill.
alarmed data.
ggmm
ed
ed@@
If the hematology analyzer detects an error, the error message appears on the screen
n
n g
g m
m as shown below.
NOTE
hhuuo
o When performing the strong cleaning as the countermeasure, after the
eennpp strong cleaning is completed, press the count switch without aspirating
g u
uyy the diluent from the sampling nozzle. Perform this procedure two or
nng more times so that the diluent replaces the CLEANAC•3 detergent inside
the hematology analyzer.
Error message when turning the power on
Error message after cleaning or priming
Error message after measurement

2. TROUBLESHOOTING
Error Messages
Messages Possible Cause/Criteria Countermeasure

A: 001 NO DILUENT
A: 002 NO CLEANAC•3 Replace the diluent, detergent or hemolysing
Out of diluent, detergent or
A: 003 NO HEMOLYNAC•3 reagent. Then press the PRIME key on the
hemolysing reagent.
A: 005 NO CLEANAC OPERATION screen.
A: 007 NO HEMOLYNAC•5
A: 021 WBC LEVEL 1 Press the Clean key to perform cleaning.
Erroneous operation during counting.
A: 022 WBC LEVEL 2 Then recount the sample.
If the alarm still occurs, perform strong
A: 023 WBC LEVEL 3 The WBC aperture cap is clogged. cleaning, clean the aperture cap and replace
the pump tube.
Press the Clean key to perform cleaning.
Then recount the sample.
A: 024 WBC CLOG The WBC aperture cap is clogged. If the alarm still occurs, perform strong
cleaning, clean the aperture cap and replace
A: 025 WBC BUBBLE 1

.ccoomm the pump tube.
A: 026 WBC BUBBLE 2

aaiill.
Air bubbles in WBC manometer.
A: 027 WBC BUBBLE 3
A: 028 WBC BUBBLE 4
ggmm Then recount the sample.
edd@@
Insufficient diluent in the manometer
e
Add the diluent or check that the connection
tube is connected properly. Perform counting
A: 029 WBC PRIME ERROR
n g
position.
n m
or the connection tube is out of
g m again after priming or cleaning the
A: 030 WBC NOISE 1

hhuuo
o
hematology analyzer.
Check the grounding, and separate the
A: 031 WBC NOISE 2
eennpp Noise is detected during counting. instrument from other equipment and their
power sources. Then recount the sample.
g u
uyy Prepare the sample and measure again.
nng
A: 032 WBC SAMPLE ERROR
A: 041 RBC LEVEL 1

Abnormal sample
Erroneous operation during counting.

Check that the temperature of the diluent is
between 15 and 30°C.
A: 042 RBC LEVEL 2 Then recount the sample.
If the alarm still occurs, perform strong
A: 043 RBC LEVEL 3 The RBC aperture cap is clogged. cleaning, clean the aperture cap and replace
the pump tube.
Then recount the sample.
A: 044 RBC CLOG The RBC aperture cap is clogged. If the alarm still occurs, perform strong
cleaning, clean the aperture cap and replace
the pump tube.
A: 045 RBC BUBBLE 1
A: 046 RBC BUBBLE 2 Press the Clean key to perform cleaning.
Air bubbles in RBC manometer.
A: 047 RBC BUBBLE 3 Then recount the sample.
A: 048 RBC BUBBLE 4
Add the diluent or check that the connection
Insufficient diluent in the manometer
tube is connected properly. Perform counting
A: 049 RBC PRIME ERROR or the connection tube is out of
again after priming or cleaning the
position.
Prepare the sample and measure again.
A: 050 RBC SAMPLE ERROR Abnormal sample Check that the temperature of the diluent is
between 15 and 30°C.
Check the grounding, and separate the
A: 051 RBC NOISE 2
Noise is detected during counting. instrument from other equipment and their
A: 052 PLT NOISE 1
power sources. Then recount the sample.
The upper part of the manometer is Press the Clean key to perform the cleaning.
A: 060 UPPER MANO ERROR
dirty. Manometer sensor output is low. Then press STRONG CLEAN on the
The lower part of the manometer is OPERATION screen to perform the strong
A: 061 LOWER MANO ERROR
dirty. Manometer sensor output is low. cleaning.
2. TROUBLESHOOTING
Error Messages Possible Cause/Criteria Countermeasure

- Adjust the HGB sensor output. Refer to
"Adjusting HGB Sensor Output Voltage" in
Section 5 "Adjustment".
HGB sensor output adjustment
A: 062 HGB VOLTAGE HIGH - Replace the CBC measuring unit with a new
error.
one if the internal connections have no
problem and the message still appears after
the adjustment.
Replace the CBC measuring unit with a new one
A: 063 HGB CIRCUIT ERROR Error in the HGB circuit. if the internal connections have no problem and
the message still appears after the adjustment.
- Check the HGB sensor output and adjust it
after cleaning the measurement bath. Refer to
"Adjusting HGB Sensor Output Voltage" in
The WBC measurement bath is
Section 5 "Adjustment".
A: 064 HGB VOLTAGE LOW dirty or the CBC measuring unit
- Replace the CBC measuring unit with a new
has a failure.
one if the internal connections have no
problem and the message still appears after
the adjustment.
A: 070 DOOR IS OPEN

.c oom
The hematology analyzer does not
m
operate because the door of the
c
Close the door (tube holder).
aaiill.
MS-721V cap pierce unit is open.
A: 071 TUBE IS CONTAINED

ggmm
Open mode can not be selected
because the sample tube is set in
Remove the sample tube and close the door (tube
holder).
eed@@
the MS-721V cap pierce unit.
d To measure WBC 5 part differential parameters,
A: 081 LASER OFF
n
n g
g m
m
The laser switch is not set to on. set the laser switch on the right side panel to on
with the laser key.
h
A: 090 ROOM TEMP OVER
huuo
o Measurement is performed at
eennpp
temperatures over 30°C.
Measurement is performed at
Perform measurement at temperatures between 15
and 30°C.
g u
uy
A: 091 ROOM TEMP LOW
y temperatures below 15°C.
nng
A: 092 CASE TEMP OVER
A: 093 CASE TEMP LOW

Internal temperature, especially at
the sheath unit, is abnormal.
-
-
Turn off the hematology analyzer. After
ventilating it, turn it on. Check the operation.
Replace the sheath unit with a new one if the
message still appears after improving the
ventilation.
Internal temperature, especially at
Turn off the hematology analyzer. After
A: 094 POWER TEMP OVER the power supply unit, is
ventilating it, turn it on. Check the operation.
abnormal.
- Set the head on the paper with the head set
lever.
The thermal head of the built-in
A: 101 WA-720VK ERROR - Replace the printer with a new one if the
printer is not ready for printing.
message still appears after setting the head on
the paper.
There is no paper in the built-in
A: 102 NO PAPER Load the paper properly.
printer.
The locally purchased external Check the printer according to the instruction
A: 103 WA-710V ERROR
printer is not ready for printing. manual of the printer.
There is no paper in the locally Load the paper properly according to the
A: 104 NO PAPER
purchased external printer. instruction manual of the printer.

2. TROUBLESHOOTING
Error Messages Possible Cause/Criteria Countermeasure

WBC 5.0?
Check that the temperature of the diluent is
RBC 4.50?
Abnormal sample between 15 and 30°C. Then recount the sample.
(? is displayed on the right of
Water and alcohol cannot be measured.
measured value.)
HGB 13.4?
The WBC measurement bath is
(? is displayed on the right of Clean the measurement bath.
dirty.
HGB measured value.)
HGB 13.4*
Needs measuring unit replacement. Contact your
(* is displayed on the right of HGB circuit error.
Nihon Kohden representative.
MCHC 38.0!
Abnormal RBC. Error occurred
(! is displayed on the right of
when diluting the sample.
MCHC measured value.)
WBC 9.0!
WBC counting error. Poor
hemolyzation.
WBC measured value.)
Check the hematology analyzer by counting the
HGB 13.4!
hematology control. Then recount the sample.
.cc om
HGB voltage adjustment error.
o m
WBC 9.0C
PLT 490C
a iill.
WBC and PLT counting error.
a
(C is displayed on the right of
gmm
Platelet coagulated. (Poor
g
hemolyzation and low PLT)
WBC and PLT measured values.)
ed
ed@@
Measurement is performed more
Replace filters
n
n g
g m
m
than 1000 times.
Measurement is performd more
Replace the filters and reset the operation history.
Replace the pump tubes and reset the operation
Replace pump tubes
hhuuo
o
than 3000 times. history.
eennpp The measurement is performed

more than 1000 times in closed Check or replace the cap pierce needle and reset
Check cap pierce needle
g u
uyy mode when the MS-721V cap the operation history.
nng pierce unit is installed.

2. TROUBLESHOOTING
System Error Messages
System Error Message Possible Cause/Criteria Countermeasure

E: 001 DILUTER INITIALIZE The diluter unit does not perform the - Replace the diluter unit with a new
ERROR initialization. one.
E: 002 BLOOD SAMPLING The diluter unit does not aspirate a blood - Replace the DRIVER board with a
ERROR sample. new one.
E: 003 DILUTER ERROR
E: 021 SAMPLER INITIALIZE The sampler unit does not perform the - Replace the sampler unit with a
ERROR initialization. new one.
E: 022 SAMPLER ERROR The sampler unit does not work. - Replace the DRIVER board with a
new one.
E: 041 WBC SUB BATH The WBC sub bath does not perform the - Replace the CBC measuring unit
INITIALIZE ERROR initialization. with a new one.
E: 042 WBC SUB BATH ERROR The WBC sub bath does not work. - Replace the DRIVER board with a
E: 051 RBC SUB BATH The RBC sub bath does not perform the new one.
INITIALIZE ERROR initialization.
E: 052 RBC SUB BATH ERROR
oomm
The RBC sub bath does not work.
.cc
E: 081 TRIPLE PUMP INITIALIZE
ERROR
aaiill.
The triple pump unit does not perform the
initialization.
- Replace the triple pump unit with a
new one.
E: 082 TRIPLE PUMP ERROR
g mm
The triple pump unit does not work.
g
- Replace the DRIVER board with a
E: 101 WASTE PUMP1 ERROR

edd@@
The pump unit does not drain the waste
e
new one.
- Replace the pump unit with a new
E: 102 WASTE PUMP2 ERROR

n
n g
g m
m
fluid from the measurement bath.
The pump unit does not drain the waste
one.
h
E: 103 PRESSURE SENSOR
huuo
o fluid from the mixing chamber.
The output voltage from the pressure
new one.
- Replace the SHEATH board with a
ERROR
eennpp sensor on the SHEATH board is out of the new one.
g u
uyy
acceptable range. - Replace the sheath unit with a new
one.
nng
E: 121 A/D CIRCUIT ERROR
E: 122 CHECK SETTINGS

The A to D converter on the AMP
CONTROL board has a failure.
The backup data is inappropriately
Replace the AMP CONTROL board
with a new one.
- Re-enter the calibration coefficient
E: 123 MEMORY ERROR changed. and all other settings. Then sample
counting can be performed.
- If the error still occurs, replace the
back-up battery with a new one.
E: 124 CIRCUIT ERROR (CBC) There is a circuit error in the CBC - Replace the CBC measuring unit
measuring unit or DRIVER board. with a new one.
new one.
E: 125 CIRCUIT ERROR (DIFF) There is a circuit error on the AMP - Replace the AMP CONTROL board
CONTROL board or DRIVER board. with a new one.
new one.
E: 141 CAP PIERCE INITIALIZE The cap pierce unit does not perform the - Replace the cap pierce unit with a
ERROR initialization. new one.
E: 142 CAP PIERCE RISING The cap pierce unit does not perform the - Replace the DRIVER board with a
ERROR upward movement. new one.
E: 144 CAP PIERCE OPERATION The cap pierce unit opens the tube holder - Check that the blood sample tube is
ERROR door during measurement. properly set in the tube holder.
- Replace the cap pierce unit with a
new one.
E: 145 TUBE HOLDER The cap pierce unit does not open the tube - Check that a blood sample tube is
OPERATION ERROR holder door when the Dispense/Open key properly set in the tube holder.
is pressed before or after measurement. - Replace the cap pierce unit with a
new one.
E: 146 CAP PIERCE RINSING The cap pierce unit does not move to the - Replace the cap pierce unit with a
ERROR position of the rinse unit. new one.
new one.
2. TROUBLESHOOTING
Inaccurate Counting and Other Problems
If operation is not accurate after attempting the countermeasure in the previous

sections, check the causes according to the following table.
Problem Possible Cause/Criteria Countermeasure

Insufficient grounding. Make sure the ground is sufficient.
Equipment near the instrument is Separate the instrument from other equipment
Noise interference during
A generating noise. and their power sources.
counting
Noise in the power line. Use a different power line.
The front cover is open. Close the front cover.
Dirty diluent. Replace the diluent.
.ccoomm
Dirty sub bath, measurement bath and Clean the sub bath, measurement bath and
filters.
aa
Dirty aperture caps.iill. filters. Refer to Section 9.
Clean the aperture caps. Refer to Section 9.
ggmm
Poor contact of external electrode to the
B High background noise
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socket of the instrument.
ee
Firmly tighten the measurement bath.
n
n g
g m
m Press the Clean key to clean the fluid path
using the CLEANAC detergent. Perform the
hhuuo
o
The fluid path and diluent tube are dirty.
strong cleaning by selecting STRONG
eennpp CLEAN on the OPERATION screen.
g u
uyy Insufficient stirring of sample.
Stir the sample sufficiently without creating
C
nng
Poor reproducibility of Dirty sub bath and/or measurement bath.
bubbles.
Clean the sub bath and/or measurement bath.
Refer to Section 9.
blood cell count
Dirty aperture caps. Clean the aperture caps. Refer to Section 9.
Reduce the background noise. Refer to
High background noise.
Problem B.
Water leaks from inside Pump tube is broken. Replace the pump tube. Refer to Section 9.
D
the hematology analyzer Filter is clogged. Replace the filter. Refer to Section 9.
Poor reproducibility of The WBC measurement bath is dirty. Clean the measurement bath. Refer to Section
E
HGB value 9.
Incorrect LCD display Circuit error. Press the Reset key. If the problem still
F Instrument repeats same occurs, turn off the instrument, wait about 10
operation seconds, then turn it on again.
Paper jammed. Remove the jammed paper. Refer to the
printer manual.
G No printing Circuit error. Press the Reset key. If the problem still
occurs, turn off the instrument, wait about 10
seconds, then turn it on again.
The pressed position and operating Calibrate the touch screen. Refer to Section 9.
position do not match.
The touch screen keys do
Circuit error. Press the Reset key. If the problem still
H not function
occurs, turn off the instrument, wait about 10
seconds, then turn it on again.

2. TROUBLESHOOTING
Problem Possible Cause/Criteria Countermeasure

Priming starts suddenly Power cord is not connected properly. Connect the power cord properly.
(When noise interferes with
the instrument program, Equipment near the instrument is Separate the instrument from other equipment
I
priming automatically starts generating noise. and their power sources.
and the READY screen
appears.) Noise in the power line. Use a different power line.
The date and time setting is not Set the correct date and time setting on the
correct. DATE & TIME screen. Refer to Section 3.
The time displayed on the Check the date and time setting on the DATE &
J upper right corner of the TIME screen and turn off and on the power of
screen is not correct. The backup battery is old. the hematology analyzer. If the time is
incorrect, replace the backup battery with a new
one. Contact your Nihon Kohden distributor.
Scattergrams appear outside Bubbles in the flow cell unit
the alloted area. Incorrect flow cell position
K Flags related to WBC 5 part Clean the flow cell unit. Refer to Section 9.
differential frequently
c oom
Incorrect gain for WBC 5 part
m
differential parameters
. c
appear.
aaiill.
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hhuuo
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eennpp
g u
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Section 3 Board/Unit Description
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CBC Measuring Unit ............................................................................................................ 3.1
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Diluter Unit .......................................................................................................................... 3.2
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Pump Unit ........................................................................................................................... 3.2
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Triple Pump Unit .................................................................................................................. 3.3
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Sampler Unit ....................................................................................................................... 3.3
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Power Supply Unit and POWER Board ................................................................................ 3.4
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Inlet/Outlet Unit ................................................................................................................... 3.4
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u o
o
Sheath Unit ......................................................................................................................... 3.5
hh u
eennpp
Front Panel Unit ................................................................................................................... 3.5
g u
uyy
Optional Printer Unit WA-720VK .......................................................................................... 3.6
Rinse Unit and Switch Assembly ........................................................................................ 3.6
nng Laser Optical Unit ................................................................................................................ 3.7
Flow Cell Adjuster ............................................................................................................... 3.7
AMP CONTROL Board ........................................................................................................ 3.7
DRIVER Board .................................................................................................................... 3.8
Optional Cap Pierce Unit MS-721V ..................................................................................... 3.8

3. BOARD/UNIT DESCRIPTION
CBC Measuring Unit
The CBC measuring unit has two measurement baths and a common manometer.
.cc omm
Each measurement bath has an aperture cap. The aperture size is different for the
o
two baths (WBC and RBC/PLT). Two electrodes are positioned so that the aperture
aaiill.
is in between them. A constant current flows between the two electrodes. When
g mm
blood cells pass through the aperture, it causes electrical resistance and the voltage
g
eed@@
between the two electrodes varies in proportion to the cell size. These voltage
d
pulses for each cell are obtained until a constant volume of diluted sample is
n g
g m
m
aspirated. For more details about counting method and hydraulic operation, refer
n
hhuuo
o to “Electric Cell Counting” and “Principle of Hydraulic Operation” in Section 10
eennpp “Reference” of the operator’s manual.
g u
uyy After the chemical processing is applied to the blood sample, the CBC measuring
nng unit measures the hemoglobin concentration when a specific wavelength light
(540 nm) is transmitted through the blood sample and diluent to the HGB sensor.
The light intensity passed through the blood sample is inversely proportional to
the hemoglobin concentration. The light intensity passed through diluent is used
as reference. The HGB sensor converts the light intensity to current. A voltage is
obtained from the current. For more details about chemical processing, refer to
“Hemoglobin Measurement” in Section 10 “Reference” of the operator’s manual.

Diluter Unit
.ccoomm
aaiill.
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ed
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The diluter unit aspirates a whole blood sample and dilutes it 200 times for WBC/
@
HGB and 40,000 times (200 x 200) for RBC/PLT with diluent. The diluter unit
n
n g m
m
supplies diluent to all the fluid paths.
g
hhuuo
o
eennpp
Pump Unit
g u
uyy
nng
There are two pump units. One is used for counting the blood cells and measuring
the hemoglobin. The other is used for WBC 5-part differential measurement.
The pump unit generates pressure to aspirate the blood sample and drain the waste
fluid.

Triple Pump Unit
.ccoomm
aaiill.
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The triple pump unit aspirates a constant volume of diluent or one of two types of
@
the hemolysing reagents and dispenses the diluent or hemolysing reagent to the
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m
specified fluid path.
hhuuo
o
e n
e npp
u yy
Sampler Unit
g u
nng
The sampler unit aspirates a whole blood sample with the WBC sampling nozzle,
moves it to the sub bath of the WBC measurement bath, and dispenses the
aspirated sample and diluent (from the diluter unit) to the sub bath so that the
sample is diluted 200 times in the sub bath. The sampler unit aspirates the 200
times diluted sample with the RBC sampling nozzle, moves it to the sub bath of
the RBC measurement bath, and dispenses the 200 times diluted sample and
diluent (from the diluter unit) to the sub bath so that the sample is diluted 40,000
times in the sub bath.

Power Supply Unit and POWER board
POWER board
.ccoomm
aaiill.
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ed
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The power supply unit has a power transformer and AC inlet socket with main
@
power switch and two fuses. The power supply unit transforms the AC line voltage
g m
m
to AC voltages. The POWER board converts the AC voltages to the DC supply
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hhuuo
o
voltages for the boards, motors and valves.
eennpp
g uuyy
nng
Inlet/Outlet Unit
The inlet/outlet unit detects the diluent, detergent and two different hemolysing
reagents. These fluids are supplied to the specified fluid path through this unit. All
waste fluids are drained through this unit.

Sheath Unit
.ccoomm
aaiill.
ggmm
ed
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The sheath unit produces a specific sample for WBC 5-part differential
@
measurement. The sample is mixed with the hemolysing reagent and injected into
g m
m
the center of the flow cell. The pressurized diluent is injected into the sheath of the
n
n g
hhuuo
o flow cell.
eennpp
Front g u
uy y
nng Unit
Panel
The front panel unit consists of the KEY DISPLAY board and VOLUME board. The
KEY DISPLAY board has key switches such as Power key and Dispense/Open key,
and LED lamps such as power lamp and operation indicator lamps. The numeric,
graphic and message data are processed on this board to display them on the LCD.
The touch screen communicates with this board. The VOLUME board has a
variable resistor for contrast control. This front unit has a space for the optional
WA-720VK printer unit.

Optional Printer Unit WA-720VK
.ccoomm
aaiill.
ggmm
ed
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The printer unit is installed into the front unit. The pinter unit prints the numeric
@
data, scattergrams and histograms on thermal roll paper.
n
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g m
m
hhuuo
o
eennp
Rinse Unit and Switch
pAssembly
g uuy
y
nng
The rinse unit cleans the sampling nozzle that blood clots can build up on the
surface. The count switch is included in this assembly.

Laser Optical Unit
Laser optical unit
Flow cell adjuster
The laser optical unit consists of optical components such as the laser diode and
.ccoom
flow cell. To differentiate WBC into 5 parts, a light scatter technique (flow
m
cytometry with laser) is used. The laser optical unit detects the light scattered by
aaiill.
the hemolyzed cells which pass in single file. The scattered light is converted to
g mm
electrical signals.
g
ed
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nnggmm
Flow Cell Adjuster
h
h u
uoo
eennpp
g u uyy
nng The flow cell adjuster adjusts the position of the flow cell in the laser optical unit
with a stepping motor.
AMP CONTROL Board
The AMP CONTROL board controls operations such as measurement and cleaning
the fluid path. This board amplifies the analog signals which are output from the
CBC measuring unit, converts the analog signals to digital signals, receives the
signals from the switches and sensors, and sends the control data to the DRIVER
board so that the valves and motors are optimally controlled.

DRIVER Board
The DRIVER board receives the control data from the AMP CONTROL board and
.ccoom
drives the valves and motors by setting the drive current to on or off.
m
aaiill.
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Optional Cap Pierce Unit MS-721V
ed
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nnggmm
hhuuoo
e n
e npp
g uuyy
nng Cap pierce needle
Cap pierce rinse unit
Tube holder door
Count switch
The cap pierce unit allows you to get the measurement data without opening the
cap of the sample tube and touching the blood.

Section 4 Disassembly and
Assembly
.cc omm
Before You Begin ................................................................................................................. 4.1
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aa ill.
Warnings and Cautions .............................................................................................. 4.1
i
Required Tools ........................................................................................................... 4.1
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Caution and Notes Related to Valve Joint, Black Screw and Tube Joint in the
d @@
Instrument ................................................................................................................. 4.2
eed
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g m
Board and Unit Location ...................................................................................................... 4.3
m
Right Side ................................................................................................................. 4.3
n
hhuuo
olest Side .................................................................................................................... 4.3
eennpp Upper Part of Right Side ............................................................................................ 4.4
g u
uyy
Lower Part of Right Side ............................................................................................ 4.4
Front View of Chassis ................................................................................................ 4.4
nng Draining the Hematology Analyzer ...................................................................................... 4.5
Opening the Front Cover ...................................................................................................... 4.7
Removing the Top Cover ...................................................................................................... 4.8
Replacing the Boot ROM ..................................................................................................... 4.9
Removing the Rear Cover Including the Power Supply Unit .............................................. 4.11
Removing the CBC Measuring Unit ................................................................................... 4.12
Removing the Diluter Unit .................................................................................................. 4.13
Removing the Sampler Unit ............................................................................................... 4.15
Removing the Pump Unit ................................................................................................... 4.16
Removing the Triple Pump Unit ......................................................................................... 4.18
Removing the WA-720VK Printer Unit (Option) .................................................................. 4.19
Removing the Front Panel Unit .......................................................................................... 4.20
Removing the Inlet/Outlet Unit .......................................................................................... 4.22
Removing the Flow Cell Adjuster ....................................................................................... 4.24
Removing the Laser Optical Unit ....................................................................................... 4.26
Removing the Sheath Unit ................................................................................................. 4.28
Removing the Rinse Unit and Switch Assembly ................................................................ 4.31
Replacing the AMP CONTROL Board ............................................................................... 4.32
Replacing the DRIVER Board ............................................................................................ 4.33
Replacing the VOLUME Board, KEY DISPLAY Board and LCD Unit ................................. 4.34
Replacing the MS-721V Cap Pierce Unit (Option) ............................................................. 4.36

4. DISASSEMBLY AND ASSEMBLY
The procedures in this section tell how to remove, replace and install major
components in the instrument.
Before You Begin
Removing, replacing and installing major components should be done by

qualified service personnel.
Warnings and Cautions

WARNINGS
• To avoid the possibility of injury to yourself or damage to the
instrument, do not install or remove any component or change switch
.cc omm
settings while the power is on and wait 10 minutes before installing to
o
aa ill.
or removing any component from the instrument after the power is off.
i
• To avoid accidental discharge of static electricity which could damage
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the instrument components, use a wrist ground strap when installing
ed
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or removing any component of the instrument.
• When replacing any parts or units in the instrument, do not touch the
n
n g
g m
m
site of the instrument where blood is or may be.
hhuuo
o • Wear rubber gloves to prevent infection by blood.
eennpp
g u
uyy
nng CAUTIONS
• Before connecting or disconnecting any cables, turn off the instrument
and unplug the AC power cord from the instrument.
• Fuses cut off the power when an abnormality occurs in the instrument.
Eliminate the malfunction before replacing the fuse. Use the correct
fuse only. The fuse rating is shown on the holder.
• Removal and replacement of any component in the instrument should
be done by qualified service personnel.
• Use only parts recommended by Nihon Kohden to assure maximum
performance from your instrument.
• Anti-static bench mat

Required Tools
• Wrist ground strap
• Phillips screwdriver (insulated type)
• Flat-blade screwdriver (insulated type)
• Tweezers

Caution and Notes Related

to Valve Joint, Black Screw CAUTION
and Tube Joint in the Valve Joint
Instrument When connecting the valve joint to the electromagnetic valve, turn the
valve joint clockwise, using moderate force until the valve joint comes
to a stop. Do not use extreme force to tighten the valve joint further
because this will damage the tip of the valve joint. If the valve joint is
loosely connected, it will leak.
Black screw
.ccoomm NOTE
aaiill.
Black screws are used to fasten the individual units to the chassis of the
g mm
instrument to enable the quick removal and replacement of these units.
g
used.
eed@@
However, to fasten the pump unit to the chassis, normal screws are
d
n
n g
g m
m
hhuuo
oSpring type tube joint
eennpp • Spring type tube joints are used in the instrument to prevent over-
g u
uyy
tightening of the joints, and to prevent loosening of the joints after the
joints are tightened.
nng
• There are 2 types of spring tube joints, white (inlet side) and black
(outlet side). Each tube joint and its corresponding port in the
instrument are marked with the same color or number to ensure
matching.

No spring type tube joint

The no spring type tube joint consists of the O-ring, tube stopper
and fitting nut. To disconnect the tube from this joint, turn the fitting
nut counterclockwise to loosen the joint and pull the tube toward you.
To reconnect the tube to this joint, insert the tube into the fitting nut and
turn the fitting nut clockwise to fasten it.
O-ring Tube Fitting Tube

stopper nut
Board and Unit Location

.ccoomm
aaiill.
ggmm
This following pictures show the location of the boards and units in the
ed
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n
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m
Right Side
hhuuo
o Inlet/outlet unit
eennpp
g u
uyy
nng
Front panel unit
Power supply unit
Sheath unit
Left Side
Laser optical unit
Flow cell adjuster
Printer unit (Option)
AMP CONTROL board

Upper Part of Right Side
Diluter unit
Lower Part of Right Side
.ccoomm
aaiill.
ggmm Triple pump unit
ed
ed@@
n
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g m
m
Front View of Chassis
hhuuo
o
eennpp Sampler unit
g u
uyy
nng Rinse unit and switch assembly
CBC measuring unit
DRIVER board Pump units

Draining the Hematology Analyzer
To avoid any trouble, drain all the fluid from the hematology analyzer. Some of the
units described in this section require the following procedure.
1. Press the STRONG CLEAN key on the OPERATIONS screen to perform strong
cleaning.
2. Disconnect the diluent tube from the ISO3 inlet, the hemolysing reagent tubes
from the HEMO3 and HEMO5 inlets, and the detergent tubes from the CLN
and CLN3 inlets on the right side panel.
CAUTION
.c oomm
Do not disconnect the waste fluid tube from the WASTE outlet.
c
aaiill.
ggmm
ed
ed@
3. Press the DRAIN ALL key on the OPERATIONS screen.
@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
A confirmation messages appears on the screen.
4. Press YES to start draining. If you press NO, the process is canceled and the
screen returns to the READY screen.

During draining, the screen shows the “DRAINING ALL” message.
When draining is completed, the “PRESS [CLEAN] KEY” message appears.
.ccoomm
aaiill.
ggmm
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n
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g m
m
hhuuo
o
eennpp
g u
uyy
nng
5. Press the main power switch on the rear panel to turn the power off. The power
lamp and main power lamp go off.
WARNING
For you safety, before disassembling the hematology analyzer, wait
approx. 10 minutes after turning off the main power switch.

Opening the Front Cover
WARNING
For your safety, before disassembling the hematolozy analyzer, wait
2. Remove the 4 screws which secure the front cover to the hematology analyzer
chassis.
.ccoomm
aaiill.
ggmm
ed
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n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
3. Slightly pull the front cover toward you and open it as shown below.
To assemble the hematology analyzer, reverse the above procedure.

Removing the Top Cover
WARNING
For your safety, before disassembling the hematolozy analyzer, wait
2. Remove the 6 screws which secure the top cover to the hematology analyzer
chassis at the right and left side.
.ccoomm
aaiill.
ggmm
ed
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n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
3. Slide the top cover rearward and remove it from the chassis.
NOTE in Assembling the Hematology Analyzer

There are hooks at the top, right and left inside of the top cover. Fit
these hooks into the front cover edge.

Replacing the Boot ROM
CAUTION
The ROM is easily damaged by static electricity. Before touching the
ROM, touch the metal chassis to eliminate static electricity from your
body.
1. If there are different settings from the default settings on the CALIBRATION
and SETTINGS screens, write down these settings.
.ccoomm
aaiill. WARNING
g mm
For your sefety, before disassembling the hematolozy analyzer, wait
g
ed
ed@
@
n
n g
g m
m
hhuuo
o 3. Remove the top cover according to the procedure described in “Removing the
eennpp
Top Cover”.
4. Insert a small flat-blade screwdriver between the ROM and its socket on the
g u
uyy AMP CONTROL board. Carefully lift the ROM with the screwdriver until the
nng ROM slightly tilts. Take out the screwdriver and insert it into the oppisite side
of the IC socket. Continue lifting both sides of the ROM with the screwdriver
until the ROM is removed.
CAUTION
When you remove the ROM from the IC socket with a sharp edge tool
such as a flat-blade screwdriver, be sure that it does not damage the
printed pattern under the IC socket.

5. Put the new ROM on the IC socket and carefully insert the pins of the new
ROM into the IC socket.
CAUTION
- Before putting the new ROM on the IC socket, check that the ROM
position is correct as shown in step 4.
- Do not break or bend a pin of the ROM. The pins must be correctly
inserted into the socket. Otherwise the hematology analyzer may not
work.
6. Put the top cover back to the original position. Refer to “Removing the Top
Cover”.
7. Turn on the main power switch on the rear panel.
.cc omm
8. Continue pressing the Reset key, Clean key and Dispense/Open key on the
o
aa ill.
front panel, press the Power key on the panel, release the Power key, and
i
release the Reset key, Clean key and Dispense/Open key.
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9. While pressing the Reset key, press the Capillary blood mode key on the front
ed
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panel. The TOUCH SCREEN CALIBRATION screen appears. Adjust the touch
screen properly. Refer to "Calibrating Touch Screen" in Section 9
n
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g m
m
"Maintenance" of the operator's manual.
hhuuo
o 10. Initialize the settings according to “Initializing Settings” in Section 3
eennpp “Changing Settings” of the operator’s manual.
g u
uyy
11. Enter the settings which you wrote down in step 1.
nng

Removing the Rear Cover Including the Power Supply Unit
WARNING
2. Remove the top cover according to the procedure described in “Removing the
Top Cover”.
3. Loosen the 2 screws at the bottom of the rear cover.
.ccoomm
aaiill.
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ed
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n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
4. Remove the 7 screws which secure the rear cover to the hematology analyzer
chassis.
5. Pull the rear cover including the power supply unit upward. Carefully separate
the rear cover from the hematology analyzer chassis and disconnect the 4
cables at the POWER board. Completely remove the rear cover from the
chassis.

Removing the CBC Measuring Unit
1. Drain all the fluid from the hematology analyzer according to the procedure
described in “Draining the Hematolozy Analyzer”.
2. Open the front cover according to the procedure described in “Opening the
Front Cover”.
3. Remove the 4 black screws which secure the valve shield cover and sampling
cup support bracket including the sampling cup. Remove them from the
Sampling cup support bracket
Sampling cup
.ccoomm
aaiill.
ggmm Valve shield cover
ed
ed@@
n
n g
g m
m
hhuuo
o 4. Remove the 4 screws which secure the CBC measuring unit to the hematology
eennpp analyzer chassis.
g u
uyy
nng
5. Release the tube tie which binds the tubes.
Tube tie

6. Disconnect the No. 1, No. 3, No. 4, No. 5 and No. 8 tube joints.
7. Disconnect the 2 flat cables from the CBC measuring unit.
8. Remove the CBC measuring unit from the chassis completely.
.c oomm
c
aaiill.
ggmm
Removing the Diluter Unit
ed
ed@@
n nggmm
hh uuoo
eennpp
g u
uy y 2. Remove the top cover according to the procedure described in “Removing the
nng Top Cover”.

3. Remove the black screw which secures the lower part of the diluter unit.
Screw at the upper part of the unit

is behind the valve
Screw at the lower part of the unit
4. Remove the black screw which secures the upper part of the diluter unit.

5. Disconnect the white tube joint and black tube joint from the diluter unit.
Black tube joint
White tube joint
6. Pull the diluter unit upward. Carefully separate it from the hematology
analyzer chassis and disconnect the cable from the diluter unit. Completely
remove the diluter unit from the chassis.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng To assemble the hematology analyzer, reverse the above procedure.
CAUTION in Assembling the Hematology Analyzer

Check that all the tube joints are firmly connected to the original
positions. Otherwise, fluid may leak.

Removing the Sampler Unit
Front Cover”.
Top Cover”.
Nozzle mover
.ccoomm
aaiill.
ggmm
ed
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n
n g
g m
m
hhuuo
o 4. Move the sampling nozzle support plate upward and move the nozzle mover
leftward as shown below so that you can remove the 4 screws which secure the
eennpp sampler unit to the chassis.
g u
uyy
nng Sampling nozzle support plate
5. Remove the 4 screws which secure the sampler unit to the chassis.
6. Release the 3 tube ties and remove the tube joint as shown below.

7. Pull the sampler unit upward until the hooks at both sides are released. Pull the
sampler unit toward you.
8. Disconnect the 2 cables from the sampler unit.
.ccoomm
9. Completely remove the sampler unit from the chassis.
aaiill.
ggmm
ed
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n
n g
g m
m
CAUTION in Assembling the Hematology Analyzer
Check that all the tube joints are firmly connected to the original
hhuuo
o positions. Otherwise, fluid may leak.
eennpp
g u
uyy
nng
Removing the Pump Unit
There are two pump units at the front of the hematology analyzer chassis. To
remove one of the pump units, perform the following procedure.
Front Cover”.
3. Remove the rear cover including the power supply unit according to the
procedure described in “Removing the Rear Cover Including the Power
Supply Unit”.

4. Remove the 3 screws which secure the pump unit to the chassis. (The
following picture shows the right pump unit about to be removed.)
5. Disconnect the motor cable of the pump unit at the DRIVER board on the rear
cover.
.ccoomm
aaiill.
Motor cable of right pump unit
ggmm Motor cable of left pump unit
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng 6. Pull the pump unit frontward and remove the pump unit from the chassis.

Removing the Triple Pump Unit
2. Remove the top cover according to the procedure described in “Removing
the Top Cover”.
3. Disconnect the 8 tube joints (No. 18 to No. 25).
19
20
22
23
21
.ccoomm 18
aaiill.
ggmm 24
ed
ed@@ 25
n
n g
g m
m
hhuuo
o
eennpp 4. Remove the 2 black screws which secure the triple pump unit to the chassis.
5. Disconnect the black tube joint (No. 12) connected to the inlet/outlet unit as
g u
uyy shown below.
nng 12
6. Carefully pull the triple pump unit frontward. Disconnect the cable from the
triple pump unit. Remove the triple pump unit from the chassis.
To assemble the hematology analyzer, reverse

the above procedure.
Removing the WA-720VK Printer Unit (Option)
WARNING
Front Cover”.
.ccoom
3. Release the 2 cable ties which bind the flat cable of the printer unit.
m
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
Cable ties
4. Remove the 4 screws which secure the printer unit to the front cover.

5. Disconnect the flat cable from the printer unit as shown below.
.c oomm
6. Completely remove the printer unit from the front cover.
c
aaiill.
ggmm
ed
ed@@
nn g
g m
m
h u
Removing the Front Panel
h uoo
Unit
eenn pp
g uuyy
nng 1. Press the main power switch on the rear panel to turn the power off. The power
WARNING
Front Cover”.
Top Cover”.
4. Remove the 4 screws which secure the front panel unit to the chassis through
the 2 hinges.
CAUTION
Hold the front panel unit immediately after removing the 4 screws.
Otherwise, the front panel unit can fall down and be damaged due to its
weight.

.c oomm
5. Release the cable tie and disconnect the cable from the front panel unit as
c
shown below.
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o Cable tie
eennpp
g u
uyy
nng
6. Completely remove the front panel unit from the chassis.

Removing the Inlet/Outlet Unit
Front Cover”.
Top Cover”.
4. Loosen the 2 black screws which secure the inlet/outlet unit to the chassis.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng 5. Release the tube tie as shown below and disconnect the No. 9 tube joint.
No. 9

6. Disconnect the No. 15 tube joint as shown below.
No. 15
oomm
7. Disconnect the 5 tube joints (No. 10 to No. 14).
.cc
No. 13
aaiill.
No. 12 No. 11 No. 10
ggmm
ed
ed@@ No. 14
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
8. Disconnect the cable from the inlet/outlet unit as shown below.

9. Slightly pull the inlet/outlet unit upward and move it rightward. Completely
remove the inlet/outlet unit.
.ccoomm
aaiill.
ggmm
Removing the Flow Cell Adjuster
ed
ed@@
nngg m
m
hhuuoo
eennpp 1. Press the main power switch on the rear panel to turn the power off. The power
g uuyy
nng WARNING
Front Cover”.
Top Cover”.
4. Remove the 2 black screws which secure the flow cell adjuster to the chassis.
2 screws

5. Gently pull the flow cell adjuster upper part upward and separate it from the
laser optical unit.
6. Disconnect the flow cell adjuster cable at the sampler unit.
.ccoomm
iill.
7. Remove the adjuster pin from the flow cell unit. Refer to “Removing the Laser
aa
ggmm
Optical Unit”.
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng

Removing the Laser Optical Unit
Top Cover”.
Supply Unit”.
4. Remove the 4 nuts (M5) which secure the laser optical unit to the chassis. Use
a suitable hex socket driver.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy 5. Remove the 2 screws which secure the flow cell unit to the holder.
nng 2 screws
Holder
6. Move the holder leftward and downward and remove it from the laser optical
unit.

7. Disconnect the 2 PharMed tubes from the single manifold.
Manifold
2 PharMed tubes
8. Disconnect the tube joint at the manifold of the sheath unit as shown below.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
9. Remove the tube joint No. 4. Release the 3 tube ties. Disconnect the cable as
shown below. Remove the laser optical unit and flow cell adjuster from the
chassis.
No. 4
Cable
NOTE
To remove only the laser optical unit. separate the flow cell adjuster
from the laser optical unit. Refer to the “Removing the Flow Cell
Adjuster”.

Removing the Sheath Unit
Top Cover”.
Supply Unit”.
3. Disconnect the 6 tube joints (No. 16 to No. 21) as shown below.
No. 19
.ccoomm No. 20
aaiill.
ggmm No. 21
ed
ed@@
n
n g
g m
m No. 17 No. 16 No. 18
hhuuo
o Black screw
eennpp
g u
uyy 5. Remove the black screw which secures the lower part of the sheath unit to the
nng
chassis.
6. Disconnect the 4 black tube joints (No. 4, No. 10, No. 13, No. 14) and white
tube joint from the diluter unit.
No. 10 No. 14
No. 13 No. 4
White tube joint from diluter unit
7. Remove the 2 black screws which secure the upper part of the sheath unit to
the chassis.

8. Disconnect the 3 cables between the sheath unit and DRIVER board.
.c oomm
9. Disconnect the 2 cables at the SHEATH board as shown below.
c
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
10. Disconnect the tube joint from the manifold of the sheath unit.

11. Remove the 2 black screws which secure the flow cell unit holder.
unit.
.ccoom
12. Move the holder leftward and downward and remove it from the laser optical
m
a iill.
13. Disconnect the 2 PharMed tubes from the single manifold.
a
ggmm Manifold
ed
ed@@
n
n g
g m
m 2 PharMed tubes
hhuuo
o
eennpp
g u
uyy
nng
14. Pull the sheath unit upward and pull it toward you.
CAUTION
When removing the sheath unit from the chassis, check that there is no
damage on the tubes and cables connected to the sheath unit.

Removing the Rinse Unit and Switch Assembly
WARNING
Front Cover”.
Top Cover”.
.ccoom
m
a iill.
4. Disconnect the 2 tube joints connected to the rinse unit.
a
ggmm
ed
ed@@
n
n g
g m
m 2 tube joints
hhuuo
o
eennpp
g u
uyy
nng
5. Release the tube tie which binds the 2 tubes.

6. Remove the 4 screws which secure the rinse unit and switch assembly to the
chassis.
7. Disconnect the white connector of the assembly as shown below.
To assemble the hematology analyzer,

reverse the above procedure.

Replacing the AMP CONTROL Board
1. If there are different settings from the default settings on the CALIBRATION
and SETTINGS screens, write down these settings.
WARNING
Top Cover”.
.ccoom
m
a iill.
4. Remove the rear cover according to the procedure described in “Removing the
a
ggmm
Rear Cover Including the Power Supply Unit”.
ed
ed@
5. Disconnect the 9 connection cables from the AMP CONTROL board.
@
6. Remove the 3 screws which secure the AMP CONTROL board to the chassis.
n
n g
g m
m
Remove the board from the chassis.
hhuuo
o
eennpp
g u
uyy
nng
7. Attach the new AMP CONTROL board to the original position with the 3
screws.

8. Connect the 9 connection cables to the AMP CONTROL board.

9. Put the rear cover and top cover back to the original positions.
10. Turn on the main power switch on the rear panel.
11. Continue pressing the Reset key, Clean key and Dispense/Open key on the
front panel, press the Power key on the panel, release the Power key, and
release the Reset key, Clean key and Dispense/Open key.
12. While pressing the Reset key, press the Capillary blood mode key on the front
panel. The TOUCH SCREEN CALIBRATION screen appears. Adjust the touch
screen properly. Refer to "Calibrating Touch Screen" in Section 9
"Maintenance" of the operator's manual.
13. Initialize the settings according to “Initializing Settings” in Section 3
“Changing Settings” of the operator’s manual.
14. Enter the settings which you wrote down in step 1.
Replacing the DRIVER Board

.ccoomm
aaiill.
ggmm
eed@@
d
n
n g
g m
m
hhuuo
o
eennpp WARNING
g u
uyy
nng
Top Cover”.
3. Remove the rear cover according to the procedure described in “Removing the
Rear Cover Including the Power Supply Unit”.
4. Disconnect the 8 connection cables from the DRIVER board.
5. Remove the 4 screws which secure the DRIVER board to the chassis. Remove
the DRIVER board from the chassis.

6. Attach the new DRIVER board to the chassis with the 4 screws.
7. Reverse the above procedure to assemble the hematology analyzer.
Replacing the VOLUME Board, KEY DISPLAY Board and LCD Unit
WARNING
.ccoomm
aaiill.
2. Remove the front panel unit according to the procedure described in
ggmm
“Removing the Front Panel Unit”.
d @@
3. Remove the 12 screws which secure the optional printer unit and shield cover
eed
n
n g
g m
to the front panel unit. Remove first the printer unit and second the shield
m
cover from the front panel unit.
hhuuo
o
eennpp
g u
uyy
CAUTION
Pay attention to the sharp edges of the shield cover. It can cause cuts or
nng injuries. To reduce these possibilities, wear gloves.
Shield cover
Printer unit

4. To remove the VOLUME board, disconnect the cable 1 at the KEY DISPLAY
board and remove the 2 screws (screws 1 and 2) from the VOLUME board.
Screw 3 Screw 12 Cable 3

Cable 2 LCD unit chassis
Screw 11 Screw 4
Screw 2 Screw 13
Screw 1
Screw 5
Screw 10
Screw 16
Screw 6
Cable 1
Screw 14
.ccoomm
Secrew 9 Screw 8
aaiill. Screw 15 Screw 7 Cable 4
ggmm NOTE
d @@
The VOLUME board is not available. If this board has a problem, only
eed
n
n g
g m
the variable resistor will be sent to you for replacement.
m
hhuuo
o 5. To remove the KEY DISPLAY board, disconnect the 4 cables (cables 1 to 4)
eennpp from the board and remove the 8 screws (screws 3 to 10) from the board.
g u
uyy 6. To remove the LCD unit, put the front panel unit face down on a table covered
nng
with a clean, soft and smooth antistatic material, disconnect the 3 cables
(cables 2 to 4) from the KEY DISPLAY board, remove the 6 screws (screws 11
to 16) from the LCD unit chassis, and remove the LCD unit chassis from the
front panel unit by pulling the LCD unit chassis toward you.
NOTE
If the front panel unit is not put face down on a flat space, the following
key top covers and spacers will be apart from the original positions.
Key top covers
Spacers
Key top covers

7. Turn the LCD unit chassis face up on the table.

8. Remove the 4 screws which secure the LCD unit to the chassis. Remove the
LCD unit from the chassis.
.ccoomm
Replacing the MS-721V Cap Pierce Unit (Option)
aaiill.
ggmm
edd@@
e
n
n g
g m
m
hhuuo
o
Front Cover”.
eennpp 3. Remove the top cover according to the procedure described in “Removing the
g u
uyy Top Cover”.
nng 4. Move the sampler unit leftward by hand.

5. Disconnect the 2 tubes from the rinse unit.
6. Disconnect the 2 cables connected to the cap pierce unit at the DRIVER board.
7. Remove the 4 screws which secure the cap pierce unit to the chassis.
8. Pull the cap pierce unit toward you.

9. Put the new cap pierce unit to the original position and reverse the above
procedure to assemble the hematology analyzer.
Section 5 Adjustment
.cc omm
Adjusting HGB Sensor Output Voltage ................................................................................ 5.1
o
aa ill.
Adjusting Upper and Lower Sensor Output Voltage of the Manometer ................................. 5.2
i
Compensating manometer Volume ...................................................................................... 5.3
ggmm
Adjusting Gain for WBC 5 Part Differential Measurement .................................................... 5.4
d @@
Counting the Polymer Microsphere Suspensions and
eed
n g
g m
Adjusting the Flow Cell Position ................................................................................ 5.5
m
Adjusting Gain for WBC 5 Part Differential Measurement Roughly
n
hhuuo
o by Polymer Microsphere Suspensions ...................................................................... 5.9
eennpp Adjusting Gain for WBC 5 Part Differential Measurement Finely
g u
uyy
by Human Blood Sample ......................................................................................... 5.10
Editing the Peak List ............................................................................................... 5.13
nng Manually Adjusting the Gain ................................................................................... 5.15
Compensating Dilution Ratio ............................................................................................. 5.15
Calibrating Touch Screen ................................................................................................... 5.16
Adjusting Liquid Sensor Output Voltages ........................................................................... 5.17
Adjusting Contrast of LCD ................................................................................................. 5.18

5. Adjustment
Adjusting HGB Sensor Output Voltage
Front Cover” of Section 4 “Disassembly and Assembly”.
2. Remove the screw which secures the HGB cover to the CBC measuring unit
chassis. Remove the HGB cover.
Screw
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o TP052 (HANA)
eennpp VR051 (HGB VR)
g u
uyy
nng
TP051 (EA)
3. Fill the sub bath for the WBC measurement bath with diluent.
4. Press the OTHER key on the MENU screen. The OTHER screen appears.
5. Press the SENSOR MONITOR key on the OTHER screen. The SENSOR
MONITOR screen which displays each sensor output voltage appears.
6. Adjust the VR051 (HGB VR) variable resistor on the HGB board so that the
HGB LED ON voltage is 3 V ±0.1 V.
7. Connect a digital voltmeter between the TP051 (EA) and TP052 (HANA) test
points and check that the voltage between the TP051 and TP052 test points is
3 V ±0.1 V. If the voltage is out of the range, adjust the VR051 (HGB VR)
variable resistor for 3 V ±0.1 V.
NOTE
If the output voltage from the sensor is less than before when the
diluent is aspirated through the aperture cap, it does not affect the HGB
measurement value but the resolution for HGB measurement decreases.
Refer to “Hemoglobin Measurement” in Section 10 “Reference” of the
operator’s manual.

5. Adjustment
NOTE in Assembling the Instrument

Before closing the front cover, check that the HGB cover is attached to
the original position.
Adjusting Upper and Lower Sensor Output Voltage of the

Manometer
Manometer window
D041 (UPPER)
.ccoomm VR041 (UP VR)
aaiill. VR042 (LOW VR)
ggmm D042 (LOWER)

TP041 (UPE)
ed
ed@@
TP012 (ED)
TP042 (LOWE)
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy 1. Open the front cover according to the procedure described in “Opening the
nng Front Cover” of Section 4 “Disassembly and Assembly”.

2. Press the OPERATIONS key on the MENU screen. The OPERATIONS screen
appears.
3. Press the PRIME key on the OPERATIONS screen. The “PRIMING” message
appears on the screen. When the WBC and RBC measurement baths fill with
diluent, watch the manometer window as shown above and check that the
manometer also fills with diluent. Also check that the two LEDs, D041
(UPPER) and D042 (LOWER), light.
6. Adjust the VR041 (UP VR) and VR042 (LOW VR) variable resistors on the
MANOMETER board so that each sensor output voltage is 1.5 V or less.
7. Press the count switch and watch the diluent level in the manometer. When the
diluent level is lower than the bottom of the manometer, press the reset key.
Check that there is no diluent in the manometer. Also check that the two LEDs,
D041 (UPPER) and D042 (LOWER), do not light.
10. Adjust the VR041 (UP VR) and VR042 (LOW VR) variable resistors so that
each sensor output voltage is 3.5 V or more.
appears.
5. Adjustment
12. Press the PRIME key on the OPERATIONS screen. The “PRIMING” message
appears on the screen. When the WBC and RBC measurement baths fill with
diluent, watch the manometer window and check that the manometer also fills
with diluent. Also check that the two LEDs, D041 (UPPER) and D042
(LOWER), light.
NOTE
• Since the threshold for fluid detection in the manometer is set to
around 2.5 V, an alarm such as UPPER MANO ERROR or LOWER
MANO ERROR can occur when the sensor output voltage comes
closer to 2.5 V.
• When the difference between the sensor output voltages with fluid and
without fluid becomes less than 2 V, a bubble alarm can frequently
occur because the difference voltage is not enough to discriminate
between the two statuses. To maintain the difference voltage 2 V or
.ccoomm
more, clean the manometer completely before checking the sensor
output voltages.
aaiill.
g
g mm
e
Compensating Manometer Volumeed
d @
@
n
n g
g m
m
hhuuo
o
eennpp
g uuyy
nng
SW012 (DIL.ADJ.1)
SW011 (DIL.ADJ.2)
Label on MC unit side
When the manometer is disassembled and assembled or replaced with a new one,
perform the compensation of the manometer volume. Refer to the following
example. The label of the default compensation value set at our factory is attached
to the CBC measuring unit.
There are two rotary switches on the PRE AMP board. Each rotary switch selects
one of 10 terminals.
The rotary switch for the first digit of the two-digit compensation value has 0.2%
increment or decrement per terminal. The rotary switch for the second digit of the
two-digit compensation value has 2% increment or decrement per terminal.

5. Adjustment
When the WBC, RBC and PLT data have 2% decreases with the hematology
control after replacing the manometer with a new one, change the compensation
value with the rotary switches so that the WBC, RBC and PLT data have 2%
increase.
If the first digit and second digit rotary switches are set to “3” and “5”,
respectively, the compensation value is “53”.
To increase the WBC, RBC and PLT data, decrease the compensation value.
In this case, to have 2% increases at WBC, RBC and PLT data, change the
compensation value “53” to “43” because 2%/0.2%=”10". Therefore, set the
second digit rotary switch to “4”.
If you change the compensation value from “53” to “60”, the WBC, RBC and PLT
data have 1.4% decrease because “7” x 0.2%=1.4%.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
Adjusting Gain for WBC 5 Part Differential Measurement
When the scattergrams appear outside their allotted area on the screen or the flags
frequently appear, adjust the gain for WBC 5 part differential measurement.
Two steps must be performed. First adjust the gain roughly by measuring 7 µm
polymer microsphere suspensions. Then adjust the gain minutely by measuring
human blood.
Observe the instructions on the 7 µm polymer microsphere suspensions manual to

achieve optimum performance and safety.

5. Adjustment
CAUTION
• Do not swallow the polymer microsphere suspensions. If swallowed,
contact your physician immediately.
• Avoid contact with the mouth and eyes. If the polymer microsphere
suspensions contacts the mouth or eyes, wash thoroughly and
immediately with water, and contact your physician immediately.
Counting the Polymer Microsphere Suspensions and Adjusting the Flow

Cell Position
The hematology analyzer uses the light scatter technique to differentiate WBC
into 5 parts. To ensure accurate laser beam scatter for the sample blood cells, count
the polymer microsphere suspensions and adjust the flow cell position.
.ccoomm
1. Clean the flow cell to remove dusts or bubbles inside the flow cell. Press the
aaiill.
CLEAN FLOWCELL key on the OPERATIONS screen to clean the flow cell.
g mm
Refer to “Cleaning the Flow Cell” in Section 9.
g
ed
ed@@ NOTE
n
n g
g m
m
After cleaning, if an alarm appears to indicate there is not sufficient
hhuuo
o amount of diluent, detergent or hemolysing reagent in the bottle, refill
eennpp the bottle and clean the flow cell again.
g u
uyy 2. Prepare about 1 mL of polymer microsphere suspensions in a sample container.
nng When the MS-721V cap pierce unit is installed, select OPEN mode on the
ORDER screen.
3. Press the OPERATIONS key on the MENU screen to display the OPERATIONS
screen.

5. Adjustment
4. Press the PARTICLE TEST key to display the PARTICLE TEST screen.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
5. Press the MEASURE key. The “PRESS [COUNT] KEY TO START” message
appears and the hematology analyzer is ready for counting the polymer
microsphere suspensions.

5. Adjustment
6. Put the sampling nozzle into the bottom of the sample container containing
the polymer microsphere suspensions so that the tip of the sampling nozzle
comes near the bottom of the sample container, and press the Count switch.
Sampling nozzle
The polymer microsphere suspensions is aspirated and measurement starts.
Count switch
The “MEASURING PARTICLES” message appears on the screen and the
scattergrams and histograms appear in real-time. The measurement lasts
Put the sampling approximately 40 seconds.
nozzle to this level
NOTE
Do not let the sampling nozzle touch the bottom of the sample
container. This may prevent aspiration of the polymer microsphere
suspensions.
The result appears on the screen.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng If the CV of FS and FL are below 7%, go to “Adjusting Gain for WBC 5 Part
Differential Measurement Roughly by Polymer Microsphere Suspensions”
section.
If the CV of FS and FL are above 7%, repeat the procedure to clean the flow
cell and measure polymer microsphere suspensions again. Then proceed to
next step.
7. Press the ADJUST FLOWCELL key on the OPERATIONS screen to display the
ADJUST FLOWCELL screen.

5. Adjustment
8. Press the MANUAL ADJUST key.
9. Press the MEAS key. The “PRESS [COUNT] KEY TO START” message
appears.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng
10. Count the polymer microsphere suspensions again. Refer to step 6.
11. Press the → or ← key to adjust the flow cell position. The histogram is
automatically redrawn. Adjust the flow cell until the CV of FS and FL is
minimum and the peak is maximum.
Adjust flow cell

position
For clearing and redrawing
scattergrams without
adjusting flow cell position

5. Adjustment
12. After adjusting the flow cell to obtain the optimum result, count the polymer
microsphere suspensions again to make sure the optimum results can be
obtained again. Refer to step 6.
If the CV of FS and FL are below 7% and the peaks match the following value, go
to “Adjusting Gain for WBC 5 Part Differential Measurement Finely by Human
Blood” section.
If the CV of FS and FL are below 7% but the peaks do not match the following
value, go to “Adjusting Gain for WBC 5 Part Differential Measurement Roughly
by Polymer Microsphere Suspensions” section.
FS Peak: 61 ±3 FL Peak: 90 ±3
Adjusting Gain for WBC 5 Part Differential Measurement Roughly by

Polymer Microsphere Suspensions
FS (Forward Small): Size (vertical
.ccoom
1. Display the SENS & THRESHOLD screen for WBC 5 part differential
m
parameters by doing one of the following:
axis of the
scattergram)
aaiill.
a)Press the GAIN key on the PARTICLE TEST screen.
FL (Forward Large): Complexity
ggmm
b)Press the SENS & THRESHOLD key on the SETTINGS screen and press the
d
(horizontal axis)
eed@@ DIFF key.
NOW: Current peak
SD (Side): Granularity
n g
g m
m
(horizontal axis)
n
AIM: Target peak
THR:
uuo
o
Threshold of FS
hh
eennpp
Press this key to
select sample type
g u
uyy
In BLOOD mode, the scattergram from PARTICLE,
nng
changes among MAIN, NE/EO,
and MO/BA when touched.
BLOOD and
CONTROL.
MEAS key in PARTICLE mode

REDRAW key in BLOOD and
CONTROL modes
2. Press the sample type key to select PARTICLE. The scattergram and the peak
for the polymer microsphere suspensions appear on the screen.
3. Press the CALC key. The optimal FS value calculated by the current (NOW)
and target (AIM) peak values appear next to VALUE.
4. Press the ENTER key to register the value to the FS box. The value now
appears next to FS and the cursor moves to FL.
5. Repeat steps 3 and 4 to register the optimal FL value.

5. Adjustment
NOTE
In PARTICLE mode, SD gain cannot be adjusted. SD gain is adjusted
in fine mode by using human blood samples.
6. Press the MEAS key to return to the PARTICLE TEST screen and count the
polymer microsphere suspensions again.
7. If the peak is not optimum, repeat the procedure in the “Counting the Polymer
Microsphere Suspensions and Adjusting the Flow Cell Position” section until
you obtain the optimum value.
NOTE
The gain adjusted by the polymer microsphere suspensions may not be
the optimum gain for human blood. Every time you adjust the gain
roughly by the polymer microsphere suspensions, adjust the gain in
.ccoom
fine mode by using human blood samples.
m
aaiill.
g mm
Adjusting Gain for WBC 5 Part Differential Measurement Finely by
g
Human Blood Sample
ed
ed@@
n
n g
g m
m NOTE
u o
o
Adjust the gain in rough mode using polymer microsphere suspensions
hh u
eennpp
before adjusting gain in fine mode.
g u
uyy 1. Measure 10 human blood samples from different healthy persons, which is 30
nng minutes to 8 hours after collection for all 22 parameters.

NOTE
• Do not use a blood sample which was collected more than 8 hours
before or refrigerated.
• Some blood samples may cause poor hemolysing when you use a
blood sample collected within 30 minutes. In this case, leave the
blood sample more than 30 minutes before measurement.
• Stir the blood sample carefully before measurement.
• Be careful that too much stirring cause poor hemolysing.
• Do not measure a blood sample which is clumped or clotted. This
may damage the hematology analyzer.
2. Press the AUTO DIFF GAIN key on the SENS & THRESHOLD screen.

5. Adjustment
The scattergram The number of data that

serial number can be registered (up to
50 samples) The sample ID
The * mark
The date and time of
appears
measurement
when the sample
is registered on The number of
the peak list. registered samples
The target MO peaks
The average peaks of

the registered MO
distribution
MO peaks of the
displayed scattergram
Actual gain
Change gain to the
optimum gain
Displays the PEAK LIST

Gain set by the AUTO
screen
key
(optimum gain) Change samples to Register the displayed sample on
.ccoomm
be displayed the peak list
aaiill.
ggmm
3. Register an appropriate blood sample for adjustment on the peak list.
Register only the blood samples which can be used for adjustment. You can
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register up to 12 samples. You should have at least 5 samples registered.
n
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m Blood samples to be registered can be displayed with the ← or → key.
hhuuo
o <Check points for blood samples which can be used for adjustment>
eennpp • The distributions of NE, LY and MO are elliptical shapes on the scattergram.
g u
uyy • The distributions of NE, LY and MO are separate from each other.
nng • NE, LY and MO should be located in the allotted positions.
Optimum scattergram
Scattergrams which cannot be used for adjustment
a)There is no NE distribution, and MO and LY are not separated on the

scattergram. (A scattergram of a leukemia patient.)
b)MO is distributed on NE. (A scattergram of a patient who has immature
granulocytes.)
c)NE is distributed too close to MO. (The scattergram of a blood sample
collected more than 8 hours ago.)

5. Adjustment
4. Press the ADD key to register the blood sample. “*” is displayed in the
scattergram serial No. after registration, and the number of N in the MO PEAK
table is incremented.
5. After registering blood samples to the peak list, check that the obtained result
is optimum on the PEAK LIST screen. Refer to the “Editing the Peak List”
section.
6. Press the AUTO key after finishing registration. The “ADJUST DIFF GAINS?”
message appears.
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7. Press the YES key to change diff gains and the peak list of the registered blood
eennpp
sample is initialized.
g u
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nng
Check the gain with a human blood sample after changing the gain.

5. Adjustment
Editing the Peak List

1. Press the LIST key on the AUTO DIFF GAIN screen to check and delete a
registered blood sample on the PEAK LIST screen.
ID keys of the registered
The number of the registered samples
samples
WBC peak CV of the

The date and time of measurement
registered samples
Peaks of MO distribution The average of WBC peak of

the registered sample
Peaks of LY distribution
Peaks of NE distribution
Delete registered sample

from the peak list
Go to another page Print the list book
.ccoomm
aaiill.
2. Press the sample ID key to return to the AUTO DIFF GAIN screen where the
ggmm scattergram of the chosen blood sample can be checked.
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m • The MO and LY scattergrams appear within the allotted area (ABCDEF) in
hhuuo
o the MAIN scattergram.
eennpp • The NE scattergrams appear within the allotted area (FEGHIJ).
g u
uyy
• The MO scattergrams appear within the allotted area (KLOP) in the MO-BA
scattergram.
nng • The LY scattergrams appear within the allotted area (LMNO) in the MO-BA
scattergram.
• The NE scattergrams appear within the allotted area (QRST) in the NE-EO
scattergram.
• Flags do not frequently appear.
MO scattergram NE scattergram MO scattergram NE scattergram
A F J K P Q T
LY scattergram L
O
E G
R S
LY scattergram
B D
I M
C H N
MAIN MO-BA NE-EO

5. Adjustment
3. To delete undesirable data from the list:

i) Press the DELETE key on the PEAK LIST screen. The “SELECT FIRST
DATA” message appears.
ii) Press the ID key of the first sample to be deleted. The “SELECT LAST
DATA” message appears.
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hhuuo
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eennpp
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iii) The confirming message for deletion appears after pressing the ID key of
the last sample to be deleted.
iv) Press the YES key to delete selected data.
4. Press the OK key on the PEAK LIST screen to return to the AUTO DIFF GAIN
screen.

5. Adjustment
Manually Adjusting the Gain

The gain can be manually adjusted.
<Adjusting the gain with automatic calculation function>

1. Measure 10 human blood samples from different healthy persons, which is 30
minutes to 8 hours after collection for all 22 parameters.
2. Press the DIFF key on the SENS & THRESHOLD screen.
Sample type
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3. Select “BLOOD” for the sample type to display the scattergram, FS, FL and SD
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m
of the blood sample counted in step 1.
hhuuo
o 4. Repeat steps 3 and 4 of the procedure in the “Adjusting Gain for WBC 5 Part
eennpp Differential Measurement Roughly by Polymer Microsphere Suspensions”
g u
uyy section to automatically adjust gain for FS, FL and SD.
nng <Gain adjustment with numeric keypad>

1. Select the gain to be changed (FS, FL, SD or THR).
2. Enter the value with the number keys. The entered value is displayed in the
VALUE box.
3. Press the ENTER key to enter the value for the selected gain.
Compensating Dilution Ratio
When the cylinder block is disassembled and assembled or replaced with a new
one, perform the compensation of the dilution ratio. Refer to the following
example. The label of the default compensation value set at our factory is attached
to the diluter unit.
There are two rotary switches on the DILUTER DRIVER board. Each rotary switch
selects one of 10 terminals.
The rotary switch for the first digit of the two-digit compensation value has 0.2%
increment or decrement per terminal. The rotary switch for the second digit of the
two-digit compensation value has 2% increment or decrement per terminal.

5. Adjustment
When the HGB and WBC data have 2% decrease with the hematology control after
replacing the cylinder block with a new one, change the compensation value with
the rotary switches so that the HGB and WBC data have 2% increase.
If the first digit and second digit rotary switches are set to “3” and “5”,
respectively, the compensation value is “53”.
To increase the HGB and WBC data, decrease the compensation value.
In this case, to have 2% increases at HGB and WBC data, change the compensation
value “53” to “43” because 2%/0.2%=”10". Therefore, set the second digit rotary
switch to “4”. Note that the RBC and PLT data have 4 (22) % increase when the
HGB and WBC data have 2% increase.
.ccoomm
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SW012 (DIL.ADJ.1)
ggmm SW011 (DIL.ADJ.2)
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hhuuo
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eennpp
g uuyy
nng Screen
Calibrating Touch
Calibrate the touch screen when the pressed position and operationg position do
not match.
1. Press the Capillary mode key while holding down the Reset key.
The TOUCH SCREEN CALIBRATION screen appears.

5. Adjustment
2. Follow the instructions on the screen to calibrate the screen.
.ccoomm
aaiill. NOTE
Do not use a sharp object to press the mark. Use your finger.
g
g mm
d @@
After calibration ins completed, the screen returns to the READY screen.
ee d
nnggmm
hhuuoo
eennp
Adjusting Liquid Sensor
p Output Voltages
g uuyy
nng
VR011 (DILUENT)
VR012 (HEMORYNAC-5)
VR013 (HEMORYNAC-3)
VR014 (SHEATH)
2. Disconnect the diluent, detergent and reagent tubes other than the waste fluid
tube from the hematology analyzer.
3. Press the Clean key on the front panel to drain all fluid from the hematology
analyzer. Alarms such as “NO DILUENT” appear on the screen.
4. Check that there is no fluid in the fluid path of the hematology analyzer.

5. Adjustment
7. Adjust the following variable resistors so that the sensor output voltages,
SHEATH, DILUENT, HEMOLYNAC-5 and HEMOLYNAC-3, displayed on the
screen are 3.5 V.
Adjusting Contrast of LCD
Turn the screen brightness control to obtain the optimal contrast of the screen.
Screen brightness control
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Section 6 Maintenance
.cc omm
To be Replaced Periodically ................................................................................................. 6.1
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Periodic Maintenance Check Procedure .............................................................................. 6.1
i
Preparation ................................................................................................................ 6.1
ggmm
Appearance ............................................................................................................... 6.1
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Safety ....................................................................................................................... 6.1
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Reagents ................................................................................................................... 6.1
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Cleaning/Replacing .................................................................................................... 6.2
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hhuuo
o SENSOR MONITOR screen ...................................................................................... 6.3
eennpp CIRCUIT CHECK Screen .......................................................................................... 6.3
g u
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Background noise ..................................................................................................... 6.4
Measuring the Polymer Microsphere Suspensions .................................................... 6.4
nng Measuring the MEK-5DN Hematorogy Control .......................................................... 6.4
Current Calibration Coefficients ................................................................................. 6.4
New Calibration Coeffisients ..................................................................................... 6.4
Software Version ....................................................................................................... 6.5
Checking the Operations ........................................................................................... 6.5
Built-in Printer Unit Operation (When the printer is installed) ..................................... 6.5
External Printer Unit Operation (When the external printer is connected) .................. 6.6
Bar Code Reader Operation (WHen the bar code reader is installed) ......................... 6.6
Others ....................................................................................................................... 6.6
Displaying Operation History Screen ................................................................................... 6.7
General ...................................................................................................................... 6.7
Displaying the OPERATION HISTORY Screen .......................................................... 6.7
Checking, Cleaning or Replacing Filters .............................................................................. 6.8
Checking and Cleaning the Sub Baths, Measurement Baths, and Sample Cup ................ 6.10
Checking and Cleaning the Rinse Unit and Sampling Nozzles .......................................... 6.12
Checking and Replacing the Pump Tubes .......................................................................... 6.14
Cleaning the Aperture Caps .............................................................................................. 6.16
Checking and Replacing the Sampling Nozzles ................................................................ 6.19
Checking and Cleaning the Cap Pierce Rinse Unit, Sampling Nozzle and
Cap Pierce Needle (Optional MS-721V Cap Pierce Unit) ................................................... 6.21
Checking and Replacing the Cap Pierce Needle (Optional MS-721V Cap Pierce Unit) ...... 6.23
Calibrating Touch Screen ................................................................................................... 6.25
Maintenance Check Sheet ................................................................................................ 6.26

6. MAINTENANCE
To be Replaced Periodically
Qty.
Description NK code
(per instrument)
O-ring for manual mode sampling nozzle 315366 1 pc.
Filter for measurement bath and air trap 6144-0018990 2 pc.
Filter packing for measurement bath and air trap 2114-082062B 2 pcs.
Pump tube 2114-080599A 2 tubes
O-ring (white) for WBC aperture cap 553198 1 pc.
O-ring (red) for RBC aperture cap 553233 1 pc.
O-ring (black) for WBC and RBC aperture caps 556827 2 pcs.
Periodic Maintenance Check Procedure
.ccoomm
aaiill.
According to Section 2 “Preparation” of the operator’s manual, turn on the power
Preparation
ggmm
and check the obtained data from the hematology analyzer before the periodic
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maintenance check by counting the diluent and MEK-5D hematology control. The
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m
obtained data must be printed out with the optional printer or written for the record
of the maintenance check. Also check that the “Priming” operation is normal after
hhuuo
o turning on the power. Prepare a container, syringe and CLEANAC·3 detergent to
eennpp clean fluid path components such as aperture cap. When calibration in capillary
g u
uyy mode is required, prepare the T857 sample cup and T812 or T813 micro cap.
nng Check that there is no damage or fluid leakage on the hematology analyzer.
Appearance
Checking the power cord:

Safety
- Check that there is no damaged AC plug and exposed wire on the power cord.
- Check that the 3-pin plug type power cord is used and the 3 pins and plug
housing are not deformed.
- Check that the resistance of the protective ground line of the power cord is 0.1
Ù or less by using an earth tester or check the continuity with a multimeter.
Check that the earth leakage current is 0.5 mA or less under normal condition.
Check that the earth leakage current is 1.0 mA or less under each single fault
condition.
Check that Nihon Kohden recommended diluent, detergent and hemolysing

Reagents
reagent are used.
Check that these reagents are used before the expiration date.
Especially, the expiration date of the Hemolynac·3 hemolysing reagent which

includes cyanogen is 30 days after the package is opened while the expiration date
of the Hemolynac·3N (non-cyanogen type) is 90 days after the package is opened.

6. MAINTENANCE
If you use Hemolynac·3 which is past the expiration date, the HGB value can be
lower than the actual value because the cyanogen is deteriorated. If Hemolynac·3
which is past the expiration date is used and the HGB value of the hematology
control is lower than the assay sheet, suggest the customer to replace the
Hemolynac·3 with a new one. If a facility does not have many blood samples and a
regular size bottle of hemolysing reagent is not consumed by one hematology
analyzer within the expiration date, suggest the customer to divide the hemolysing
reagent into several smaller bottles for several hematology analyzers.
Cleaning/Replacing 1. Press the STRONG CLEAN key on the OPERATIONS screen to start strong
cleaning.
2. Disconnect the diluent, detergent and reagent tubes other than the waste fluid
tube from the right side panel of the hematology analyzer.
3. Press the DRAIN ALL key on the OPERATIONS screen to drain all the fluid
inside the hematology analyzer.
.ccoomm
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4. Turn off the main power switch on the rear panel.
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6. Perform the following checking, cleaning and replacing.
eed
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Cleaning the rinse unit:
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hhuuo
o
Clean the rinse unit according to the procedure described in “Checking and
een pp
Cleaning the Rinse Unit and Sampling Nozzles” in this section.
n
g u
uyy
nng
Cleaning the cap pierce needle:
Clean the cap pierce needle according to the procedure described in “Checking
and Cleaning the Cap Pierce Rinse Unit, Sampling Nozzle and Cap Pierce Needle”
in this section.
Replacing the filters:

Replace the filters with new ones according to the procedure described in
“Checking, Cleaning or Replacing the Filters” in this section.
Replacing the pump tubes:

Replace the pump tubes with new ones according to the procedure described in
“Checking and Replacing the Pump Tubes” in this section.
Cleaning the sub baths, measurement baths and sampling cup:

Clean the sub baths, measurement baths and sampling cup according to the
procedure described in “Checking and Cleaning the Sub Baths, Measurement
Baths, and Sampling Cup” in this section.
Cleaning the aperture caps:

Clean the aperture caps according to the procedure described in “Cleaning the
Aperture Caps” in this section.
Cleaning the touch screen:

Clean the touch screen with a soft cloth moistened with 80% alcohol.

6. MAINTENANCE
Checking and replacing the sampling nozzles:

Check the sampling nozzles and replace them with new ones if they are damaged
or PLT background noise increases according to the procedure described in
“Checking and Replacing the Sampling Nozzles” in this section.
SENSOR MONITOR Screen 1. Turn on the main power switch on the rear panel.
2. While pressing the Reset key on the front panel, press the Power key on the
front panel. The power turns on without priming. The READY screen appears.
3. Press the MENU key on the READY screen. The MENU screen appears.
6. Check that the sensor output voltages of MANO UPPER, MANO LOWER,
DILUENT, HEMOLYNAC-5 and HEMOLYNAC-3 are 3.5 V or more under no
.cc omm
fluid condition. Write down each voltage on the check sheet.
o
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7. Connect the diluent, detergent and reagent tubes to the right side panel of the
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appears.
9. Press the PRIME key on the OPERATIONS screen. The screen returns to the
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READY screen after priming operation is completed.
hhuuo
o 10. Check that the following sensor output voltages are displayed on the SENSOR
eennpp MONITOR screen. Write down each voltage on the check sheet. If there is a
g u
uyy
sensor output voltage out of the acceptable range, adjust the sensor output
voltage for the acceptable range according to Section 5 “Adjustment”.
nng MANO UPPER, MANO LOWER, DILUENT, HEMOLYNAC-5 and

HEMOLYNAC-3: 1.5 V or less
ELECTRODE: 17.7 V to 18.3 V
HGB LED ON: 1.5 V to 4.5 V (Adjust this voltage for 2.9 V to 3.1 V if it is out
of 1.5 V to 4.5 V.)
HGB LED OFF: 0.5 V or less
CIRCUIT CHECK Screen 1. Press the MENU key on the READY screen. The MENU screen appears.
3. Press the CIRCUIT CHECK key on the OTHER screen. The CIRCUIT CHECK
screen which displays the check result of specified measurement parameters
appears.
4. Check that the following values are displayed on the CIRCUIT CHECK
screen.
WBC: 8.0±5%
RBC: 1.6±5%
HGB ON: 1.5 V to 4.5 V
HGB OFF: 0.5 V or less
MCV: 100±15%
PLT: 160±5%

6. MAINTENANCE
Sensitivity and threshold

WBC: 5 (sensitivity), 4 (threshold)
RBC: 5 (sensitivity), AT (AUTO) or 5 (threshold)
PLT: 5 (threshold)
Background noise 1. Press the count switch to count the diluent so that the background noise is
checked. Refer to “Measuring Background Noise” in Section 2 “Preparation”
of the operator’s manual. The measurement result appears.
2. Check that the following values are displayed on the screen.
WBC: 0.2 or less (x 103/ìL)
RBC: 0.05 or less (x 106/ìL)
HGB: 0.1 or less (g/dL)
PLT: 10 or less (x 103/ìL)
TOC: 100 or less
.ccoomm
Measuring the Polymer aaiill.
1. Measure the polymer microsphere suspensions according to the procedure
Microsphere Suspensions
ggmm
described in “Counting the Polymer Microsphere Suspensions and Adjusting
d @@
the Flow Cell Position” in Section 3 “Changing Settings” of the operator’s
eed
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manual.
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2. Check that the CV of FS and FL are 7% or less on the PARTICLE TEST screen.
n
hhuuo
o
If the CV is more than 7%, clean the flow cell, measure the polymer
eennpp microsphere suspensions again, and adjust the flow cell position.
g u
uyy
nng
Measuring the MEK-5DN
Hematology Control
1. Write down the lot number of the MEK-5DN hematology control on the check
sheet.
2. Check the reproducibility by measuring the MEK-5DN hematology control.
Refer to “Counting the MEK-5D Hematology Control” in Section 6 “Quality
Control” of the operator’s manual.
3. Check that the obtained results are within the acceptable range. If the obtained
data is out of the range, go to “New Calibration Coefficients”.
Current Calibration 1. Press the CALIBRATION key on the MENU screen. The CALIBRATION
Coefficients screen appears.
2. Press the VENOUS key or CAPILLARY key on the CALIBRATION screen
according to the facility’s requirement. The current calibration coefficients are
displayed on the screen.
3. Write down the calibration coefficients on the check sheet if the obtained
results are within the acceptable range at “Measuring the MEK-5DN
Hematology Control” in this section.
New Calibration 1. Write down the lot number of the MEK-3DN hematology control on the check
Coefficients sheet.
2. Measure the MEK-3DN hematology control three times. Refer to “Measuring
Calibrator” in Section 7 “Calibration” of the operator’s manual.

6. MAINTENANCE
3. Calibrate the hematology analyzer by entering the new calibration coefficient

or measured data. Refer to “Calibration in Venous Blood Mode” or
“Calibration in Capillary Blood Mode” in Section 7 “Calibration” of the
operator’s manual.
NOTE
Before entering the new calibration coefficient or measured data, ask the
customer if you can change the setting. Some customers calibrate the
hematology analyzer for data consistency with other manufacturers’
hematology analyzers.
Software Version 1. Press the OTHER key on the MENU screen. The OTHER screen appears.
2. Press the OPERATION HIST key on the OTHER screen. The OPERATION
HISTORY screen appears. It displays the software version, total operating time,
total number of counts, and number of counts that filters and pump tubes are
.cc omm
used. Refer to “Displaying Operation History Screen” in this section.
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3. Write down the software version on the check sheet.
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Checking the Operations
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Touch screen
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Check the touch screen function. Refer to “Calibrating Touch Screen” in this
m
section.
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hhuuo
o
eennpp Date and time
g u
uyy
Check the date and time settings. Refer to “Setting Date and Time” in Section 3
nng
“Changing Settings” of the operator’s manual.
Automatic cleaning operation

Check that the automatic cleaning operations are normal during this maintenance
check.
Fluid leakage
Check that there is no fluid leakage on the hematology analyzer, especially after
each automatic cleaning operation.
Dispense operation
Check that the constant volume of the diluent is properly dispensed when pressing
the Dispense/Open key on the front panel in capillary blood mode.
Open/Closed mode selection operation (when the cap pierce unit is

installed)
Check that the cap pierce unit properly works when selecting the Open mode or
Closed mode.
Built-in Printer Unit Check that Pressing the Feed key on the front panel feeds the recording paper and
Operation (When the there is no dot missing on the paper by pressing the Print key on the front panel.
printer is installed)

6. MAINTENANCE
External Printer Unit Check that the paper feed operation properly works on the external printer and
Operation (When the there is no dot missing on the paper.
external printer is
connected)
Bar Code Reader 1. Clean the light emitter and detector parts with a cotton swab moistened with
Operation (When the bar 80% alcohol.
code reader is installed) 2. Check that the bar code reading operation properly works.
Others Write down any other points on the check sheet.
.ccoomm
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6. MAINTENANCE
Displaying Operation History Screen
General You can display the total operating time, total number of counts, and number of
counts that filters and pump tubes are used to determine the maintenance schedule.
When filters or tubes are used more than the following number of sample counts,
the messages appears on the READY screen to prompt you to check and replace
them.
Filters: 1000 counts
Pump tubes: 3000 counts
Cap pierce needle: 1000 counts
Displaying the
OPERATION HISTORY
c oom
1. Press the OPERATION HIST key on the OTHER screen to display the
m
OPERATION HISTORY screen.
. c
Screen
aaiill.
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Total number of counts
Total operating hours
Number of counts
the filters are used
Number of counts the

pump tubes are used
Number of counts the cap pierce
needle is used (only when the
cap pierce unit is installed)
The screen shows the total operating time, total number of counts, and number
of counts that filters and pump tubes are used.
After checking and replacing filters or tubes and the cap pierce needle, reset
the counts to zero by pressing the RESET key.
2. Press the OK key to return to the OTHER screen.

6. MAINTENANCE
Checking, Cleaning or Replacing Filters
Check and clean the filters once a week or every 300 sample counts. Replace them
when they are clogged or dirty (every 1000 sample counts).
cleaning.
2. Press the DRAIN BATHS key on the OPERATIONS screen to drain the
measurement baths and sub baths.
3. Press the Power key while holding down the Reset key to turn the power off.
.ccoom
Check that the power lamp is off.
m
aaiill.
ggmm
ed
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hhuuo
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eennpp 5. Open the front cover by pulling it from the right side.
g u
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nng
6. Remove the 2 filter joint assemblies by turning the tube

connectors.

6. MAINTENANCE
Filter 7. Remove the filter from each assembly. Use tweezers to remove any dust from
the filter. If it is still dirty, replace it with a new one.
8. Reattach the filter joint assemblies.
Filter packing
NOTE
• When attaching the filter joint assembly, be careful not to bend or
damage the filter packing at the bottom of the measurement bath.
• When there is a leakage, check that there is no scratch or damage
around the filter.
.ccoomm
aaiill.
9. Reattach the front cover and fasten it with the two screws on each side.
ggmm
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10. On the OPERATION HISTORY screen, press the RESET key for FILTERS to
d reset the counts to zero.
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hhuuo
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eennpp
g u
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6. MAINTENANCE
Checking and Cleaning the Sub Baths, Measurement Baths, and

Sample Cup
Check the sub baths, measurement baths, and sample cup every day.
Clean the measurement baths, sub baths, and sample cup when there is any blood
stain or dust on them. (Once a month or every 1000 sample counts)
NOTE
Be careful not to damage the sub baths and measurement baths. The
baths are made of special plastic.
.c oomm
c
cleaning.
aaiill.
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@
e
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hh uo
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u
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4. Remove the two screws on each side of the front cover of the hematology
analyzer.
5. Open the front cover by pulling it from the right side.

6. MAINTENANCE
6. Check the WBC and RBC measurement baths, sub baths and sample cup. If
there is any blood stain or dust on them, remove and clean them taking the
following steps.
Sample cup 7. Remove the joint of the sample cup.
Tube
8. Hold the tabs of the sample cup and push the
cup back to remove it.
9. Remove the HGB cover of the WBC

measurement bath by removing the screw.
Joint
Sub baths
10. Loosen the screws fastening the measurement

baths. (The screws cannot be removed from the
.ccoomm
measurement baths.)
aaiill. 11. Remove the measurement baths and sub baths
ggmm by pulling them together.
ed
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m sample cup in CLEANAC•3 detergent for about
hhuuo
o 10 minutes.
eennpp WBC measurement bath

13. Rinse the measurement baths, sub baths, and
uyy
RBC measurement bath
g u sample cup with water and wipe them with a dry
nng cloth.
14. Reattach the baths and cup to its original

position and tighten screws. Make sure that the
HGB cover
baths are attached firmly, especially the sub
baths.
Measurement bath screws
15. Replace the HGB cover and fasten it with the

screw.
16. Reattach the front cover and fasten it with two screws on each side.
17. Turn on the power. The hematology analyzer starts priming the fluid path.

6. MAINTENANCE
Checking and Cleaning the Rinse Unit and Sampling Nozzles
Check and clean the rinse unit and sampling nozzles once a month or every 1000
sample counts.
cleaning.
.ccoomm
aaiill.
ggmm
ed
ed@@ 4. Remove the two screws on each side of the front cover
n
n g
g m
m of the hematology analyzer.
hhuuo
o 5. Open the front cover by pulling it from the right side.
eennpp
g u
uyy
nng
6. Remove blood or salt crystals on the rinse unit cap and the tip of the sampling
nozzles with a cotton swab or cloth moistened with CLEANAC•3 detergent.
Rinse unit cap
Sampling
nozzles
Rinse unit
Tip of sample nozzles

6. MAINTENANCE
Sampling nozzle plate 7. Slide the sampling nozzle plate to the left.
8. Turn the rinse unit cap counterclockwise to remove the rinse

unit.
9. Loosen the joint assemblies to remove the tubes. Be careful not

to lose the O-ring from the rinse unit.
Rinse unit cap
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Joint
assemblies
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ggmm
ed
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Rinse unit
n
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m
hhuuo
o 10. Insert a cotton swab into the rinse unit from the bottom and push out the O-
eennpp
ring to remove it from the rinse unit.
g u
uyy
nng
11. Wipe the inside of the rinse unit and rinse unit cap with a cotton swab
moistened with CLEANAC•3 detergent. If they are still dirty, soak them in
CLEANAC•3 for about 10 minutes.
12. Rinse the rinse unit, rinse unit cap, and O-ring with water and dry thoroughly
with a dry cloth.
13. Reattach the O-ring to the rinse unit and return the rinse unit and rinse unit cap
to the original position.
15. Turn the power on. The hematology analyzer starts priming the fluid path.

6. MAINTENANCE
Checking and Replacing the Pump Tubes
Check the pump tubes for water droplets and leaks every day.
Replace the pump tubes when there are water droplets or leaks. (Once every 4
months or every 3000 sample counts)
NOTE
Do not leave the pump tubes with water droplets or leaks on them. This
may erode them.
cleaning.
.ccoomm
a iill.
a
ggmm
ed
ed@@
n
n g
g m
m Check that the power lamp is off.
hhuuo
o
eennpp
g u
uyy 4. Remove the two screws on each side of the front cover of the
nng hematology analyzer.
6. Check the pump tubes for water droplets and leaks. If any
droplet or leak is found, replace the tube with a new one taking
the following steps.
7. Remove the pump covers by removing the two screws on each

cover.
Pump covers
Pump tube Pump tube

6. MAINTENANCE
8. Pull out the white tube joint from the tube holder and pull out the
White
Pump rotator tube by turning the pump rotator counterclockwise. Then pull out
the black tube joint from the tube holder.
Tube holder Black
9. Remove the white and black tube joints and replace the pump tube
with a new one.
.ccoomm
aaiill.
ggmm
Tube guide
ed
ed@@ 10. Put the white tube joint back to the original position and push the
n
n g
g m
m
pump tube into the tube guide by turning the rotator counterclockwise.
hhuuo
o 11. Put the black tube joint back to the original position.
eennpp
g u
uyy
nng NOTE
• Do not damage the pump tube with the tube guide.
• Do not attach the black tube joint to the tube holder before the
white tube joint. This causes disconnection of the tube due to
compressed internal air.
• Put back the pump tube properly. If the pump tube has slack, it
will be damaged by the tube guide.
• Make sure the joints are held properly by the tube holder as
shown below. Otherwise, the life of the pump tube will be
shortened.
14. Display the OPERATION HISTORY screen and press the RESET key for
TUBES to reset the counts to zero.

6. MAINTENANCE
Cleaning the Aperture Caps
For daily cleaning of the aperture caps, press the Clean key on the front panel.
However, if the “clogged” messages frequently appears or background noise is still
high, clean the aperture caps taking the following steps.
cleaning.
2. Remove the diluent tube from the ISO3 inlet, the hemolysing reagent tubes
from the HEMO3 and HEMO5 inlets, and the detergent tubes from the CLN
and CLN3 inlets on the right side panel. Do not disconnect the waste fluid
tube from the WASTE outlet.
.c oomm
3. Press the DRAIN ALL key on the OPERATIONS screen. A confirmation
c
a iill.
message appears on the screen.
a
ggmm
ed
ed@
4. Press YES to start draining the hematology analyzer.
@
n
n g
g m
m
hhuuo
o
eennp
5. After draining, press the Power key while holding down the Reset key to turn
pthe power off. Check that the power lamp is off.
g u
uyy
nng
analyzer.

6. MAINTENANCE
Sub baths 8. Remove the HGB cover of the WBC measurement

bath by removing the screw.
9. Loosen the screws fastening the measurement baths.

(The screws cannot be removed from the
measurement baths.)
WBC
measurement bath
RBC
measurement bath
10. Remove the measurement baths and sub baths by
pulling them together.
.ccoomm
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HGB cover
ggmm
HGB cover screws
ed
ed@@
Measurement bath screws
n
n g
g m
m
hhuuo
o
eennpp 11. Place a cloth or tissue paper under your hand and remove the aperture
g u
uyy cap by pulling it toward you. If it is not easy to pull the aperture cap,
nng move it slowly left and right to remove it.
12. Carefully rinse the aperture cap. Remove all dirt, especially from the
inside.
Syringe 13. Insert a syringe containing CLEANAC•3 detergent into the aperture cap.
Concave Aspirate and dispense the detergent by pressing the syringe’s plunger up
and down to remove the dust around the aperture cap.
NOTE
Be careful not to press the syringe with too much force.
Otherwise the aperture cap may be damaged.
Aperture
The condition of the aperture cap can be checked with a 100×

microscope.

6. MAINTENANCE
14. If clogged dust still remains in the aperture caps, soak the
aperture caps in CLEANAC•3 detergent for about an hour.
CAUTION
Handle the aperture caps with care. It may be damaged if a
sharp object is used to clean it.
Red mark
15. Rinse the aperture caps with water and reattach them to the
White mark
original position.
• There are separate aperture caps for WBC and RBC. Be careful
not to attach the aperture caps to the wrong positions.
Attach the WBC aperture cap (white O-ring) to the white side.
.ccoom
Attach the RBC aperture cap (red O-ring) to the red side.
m
• Check that the red or white O-ring is at the front.
Black O-ring
aaiill.
Red O-ring Black O-ring
g mm
16. Return each bath to the original position and tighten the screws.
g
White O-ring
ed
ed@@
Make sure that the baths are attached firmly, especially the sub
baths.
n
n g
g m
m
hhuuo
o 17. Fasten the HGB cover with the screw.
eennpp
g u
uyy
18. Reattach the front cover and fasten it with the two screws on each
side.
nng 19. Turn the power on. The hematology analyzer starts priming the
fluid path.
20. Count a diluent sample and check that the background noise is
decreased. Refer to “Measuring Background Noise” in Section 2.

6. MAINTENANCE
Checking and Replacing the Sampling Nozzles
Check the sampling nozzles once a month or every 1000 sample counts taking the
following steps.
When PLT background noise increases, replace the sampling nozzles with a new
one.
The plastic part of the sampling nozzle for the MEK-7222J/K hematology analyzer
is colored black to avoid misuse. Do not use the sampling nozzle which has a gray
plastic part.
cleaning.
.ccoom
m
aaiill.
ggmm
ed
ed@
@
g m
m
n
n g
hhuuo
o Check that the power lamp is off.
eennpp
g u
uyy
nng
analyzer.

6. MAINTENANCE
Sampling nozzle screws 6. Check the sampling nozzles for blood stains, flaking of
the coating, or bending. There are two sampling nozzles.
If the sampling nozzle needs to be replaced, take the
Tube holder following steps.
Spacers 7. Remove the screw, tube holder, and spacer from the
sampling nozzle.
NOTE
Be careful not to drop the screws, tube holder and
spacers into the hematology analyzer.
.ccoomm
aaiill.
ggmm
8. Remove the joint from the sampling nozzle.
Sampling nozzle
ed
ed@@
Joint
n
n g
g m
m
hhuuo
o 9. Turn the sampling nozzle 90° counterclockwise and pull
eennpp it up to remove it.

Sampling nozzle
g u
uyy
nng
Joint
10. Insert the new sampling nozzle into the sampling nozzle
guide, attach the joint and fasten the nozzle with the
screw.
11. Reattach the front cover and fasten it with the two screws
on each side.
12. Turn the power on. The hematology analyzer starts

Sampling nozzle priming the fluid path.
guide

6. MAINTENANCE
Checking and Cleaning the Cap Pierce Rinse Unit, Sampling

Nozzle and Cap Pierce Needle (Optional MS-721V Cap Pierce Unit)
Check and clean dried blood and dirt on the cap pierce rinse unit, sampling
nozzles and cap pierce needle every month or every 1000 sample countings.
cleaning.
.c oomm
c
aaiill.
ggmm
ed
ed@@
n
n g
g m
m 4. Remove the two screws on each side of the front cover of the
hhuuo
o hematology analyzer.
eennpp
g u
uyy
nng
6. Check for dried blood or dirt on (1), (2), (3) and the bottom of the cap pierce
rinse unit as shown below.
Cap pierce unit
Cap pierce needle cover
(2)
Cap pierce rinse unit
Cap pierce rinse
(1) unit
(3)
(1) Check for dirt around the opening
of the cap pierce rinse unit (3) Check for dirt on the
(2) Check for dirt around the opening of sampling nozzle (especially
the cap pierce needle cover tip of the nozzle)
Wipe any dirt off (1), (2), (3) and the bottom of the cap pierce rinse unit with
the cotton swab if there is dried blood or dirt.
6. MAINTENANCE
NOTE
To remove crystals of blood or salt from the sampling nozzles, wipe
them off with a gauze or a cotton swab moistened with CLEANAC•3
detergent.
9. Display the OPERATION HISTORY screen and press the RESET key for
NEEDLE to reset the counts to zero.
.ccoomm
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ggmm
ed
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n
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g m
m
hhuuo
o
eennpp
g u
uyy
nng RESET key

6. MAINTENANCE
Checking and Replacing the Cap Pierce Needle (Optional MS-

721V Cap Pierce Unit)
Check that there is no blood clot, dirt or damage on the cap pierce needle every
month or every 1000 sample counts.
If there is any damage to the cap pierce needle, replace it with a new one according
to the following procedure.
WARNING
The cap pierce needle is sharp and sample blood may have contacted it.
Be careful not to prick yourself with the cap pierce needle.
.ccoomm
aaiill.
ggmm
ed
ed@@ 1. Press the STRONG CLEAN key on the OPERATIONS
n
n g
g m
m
screen to perform strong cleaning.
hhuuo
o
eennpp
g u
uyy 2. Press the DRAIN BATHS key on the OPERATIONS screen
nng to drain the measurement baths and sub baths.
3. Press the power key while holding down the reset key to
turn the power off. Check that the power lamp is off.
4. Remove the two screws on each side of the front cover of


6. MAINTENANCE
Sampling nozzle plate 6. Slide the sampling nozzle plate to the left.
7. Remove the screw from the cover.

Cover
Pull the cover towards you and remove it from the cap pierce
needle.
Cap pierce needle
.ccoomm
aaiill.
Cap pierce
rinse unit
ggmm
ed
ed@@
n
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g m
m
Tube joint
hhuuo
o 8. Pull the cap pierce needle upwards and remove it from the
eennpp cap pierce rinse unit. Turn the tube joint counterclockwise
g u
uyy
and disconnect it from the cap pierce needle.
Cap pierce needle

nng
Car pierce 9. Replace the cap pierce needle with a new one.
rinse unit
10. Assemble the cap pierce needle cover and put back the
sampling nozzle plate to its original position.
11. Reattach the front cover and fasten it with the two screws on
each side.
12. Turn the power on. The hematology analyzer starts priming
the fluid path.
13. Display the OPERATION HISTORY screen and press the

RESET key for NEEDLE to reset the counts to zero.

6. MAINTENANCE
Calibrating Touch Screen
Calibrate the touch screen when the pressed position and operating position do
not match.
1. Press the Capillary mode key while holding down the Reset key.
The TOUCH SCREEN CALIBRATION screen appears.
.ccoomm
aaiill.
ggmm
ed
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n
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g m
m
hhuuo
o
eennpp
g u
uyy
nng 2. Follow the instructions on the screen to calibrate the screen.
NOTE
Do not use a sharp object to press the mark. Use your finger.
After calibration is completed, the screen returns to the READY screen.

6. MAINTENANCE
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ggmm
This Page is intentionally left blank.
ed
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m
hhuuo
o
eennpp
g u
uyy
nng

6. MAINTENANCE
Maintenance Check Sheet

Fill out and save this check sheet each time you do maintenance or service.
Date:
Customer:
Customer Address:
Service Personnel: Service Company:
Instrument Name: Hematology Analyzer Instrument Model: MEK-7222J K

Instrument Serial Number: Hardware Revision:
Software Version:
Appearance
c oomm
There is no damage on the hematology analyzer.
. c
Yes No
aaiill.
There is no fluid leakage on the hematology analyzer. Yes No
ggmm
Safety
edd@@
There is no damaged AC plug and exposed wire on the power cord.
e Yes No
n
n g
g m
m
3-pin plug type power cord is used and the 3 pins and plug housing are not deformed. Yes No
u o
Resistance of the protective ground line of the power cord is 0.1 Ù or less.
o Yes No
Cut here
n pphh u
Earth leakage current is 0.5 mA or less under normal condition. Yes ( mA) No
uyyee n
Earth leakage current is 1.0 mA or less under each single fault condition. Yes ( mA) No
g
nng u
Reagents
Nihon Kohden recommended diluent, detergent and hemolysing reagent are used. Yes No
ISOTONAC·3 diluent is not past the expiration date. Yes No
CLEANAC detergent is not past the expiration date. Yes No
CLEANAC·3 detergent is not past the expiration date. Yes No
HEMOLYNAC·3 or HEMOLYNAC·3N hemolysing reagent is not past the expiration date. Yes No
HEMOLYNAC·5 hemolysing reagent is not past the expiration date. Yes No
Cleaning/Replacing
Rinse unit is cleaned. Yes No
Cap pierce needle is cleaned. Yes No
Two filters are replaced with new ones. Yes No
Two pump tubes are replaced with new ones. Yes No
Sampling cup (Bath for WBC 5 part differential measurement) is cleaned. Yes No
Two sub baths and two measurement baths are cleaned. Yes No
Two aperture caps are cleaned. Yes No
Touch screen is cleaned with a soft cloth moistened with 80% alcohol. Yes No
Two sampling nozzles are cleaned or replaced with new ones if they are damaged or
PLT background noise increases. Yes No

6. MAINTENANCE
SENSOR MONITOR screen

Write each sensor output voltage on the SENSOR MONITOR screen as follows:
- MANO UPPER: _____V under no fluid condition (Acceptable range: 3.5 V or more)
- MANO LOWER: _____V under no fluid condition (Acceptable range: 3.5 V or more)
- DILUENT: _____V under no fluid condition (Acceptable range: 3.5 V or more)
- HEMOLYNAC-5: _____V under no fluid condition (Acceptable range: 3.5 V or more)
- HEMOLYNAC-3: _____V under no fluid condition (Acceptable range: 3.5 V or more)
- MANO UPPER: _____V filled with fluid (Acceptable range: 1.5 V or less)
- MANO LOWER: _____V filled with fluid (Acceptable range: 1.5 V or less)
- DILUENT: _____V filled with fluid (Acceptable range: 1.5 V or less)
- HEMOLYNAC-5: _____V filled with fluid (Acceptable range: 1.5 V or less)
- HEMOLYNAC-3: _____V filled with fluid (Acceptable range: 1.5 V or less)
- HGB LED ON: _____V with fluid (Acceptable range: 1.5 V to 4.5 V)
- HGB LED OFF: _____V with no fluid (Acceptable range: 0.5 V or less)
- ELECTRODE: _____V filled with fluid (Acceptable range: 17.7 V to 18.3 V)
.ccoom
- MJ (Warmer temperature): _____°C filled with fluid
m
- CD (Temperature inside chassis): _____°C filled with fluid
aaiill.
- DILUTER CF: _____ (Compensation of dilution ratio)
g mm
- MEAS UNIT CF: _____ (Compensation of manometer volume)
g
CIRCUIT CHECK screen
ed
ed@@
n g
g m
m
Write each value on the CIRCUIT CHECK screen as follows:
n
u o
o
Cut here
- WBC: ______(Acceptable range: 8.0±5%)
pphh u
- RBC: ______(Acceptable range: 1.6±5%)
n
uy ee n
- HGB ON: ______(Acceptable range: 1.5 V to 4.5 V)
y
g u
- HGB OFF: ______(Acceptable range: 0.5 V or less)
nng
- MCV: ______(Acceptable range: 100±15%)
- PLT: ______(Acceptable range: 160±5%)
- Sensitivity: WBC: (___), RBC: (___)
- Threshold: WBC: (___), RBC: (___), PLT: (___)
Background noise check

Write each value displayed on the screen after pressing the count switch:
- WBC: ____ (Acceptable range: 0.2 or less (x 103/ìL))
- RBC: ____ (Acceptable range: 0.05 or less (x 106/ìL))
- HGB: ____ (Acceptable range: 0.1 or less (g/dL))
- PLT: ____ (Acceptable range: 10 or less (x 103/ìL))
- TOC: ____ (Acceptable range: 100 or less)
Check with polymer microsphere suspensions

Write each value on the PARTICLE TEST screen when the polymer microsphere suspensions are aspirated with
the sampling nozzle.
- FS CV%: ___ (Acceptable range: 7% or less)
- FS PEAK: ___
- FL CV%: ___ (Acceptable range: 7% or less)
- FL PEAK: ___
- TOC: ___

6. MAINTENANCE
Check with MEK-5DN Hematology Control

Write down the lot number of the MEK-5DN hematology control and all the X-R data on the X-R (NORMAL)
screen when the hematology control is aspirated with the sampling nozzle.
Lot No. of MEK-5DN: _____________
- WBC: X ____, R ____
- NE%: X ____, R ____
- LY%: X ____, R ____
- MO%: X ____, R ____
- EO%: X ____, R ____
- BA%: X ____, R ____
- RBC: X ____, R ____
- HGB: X ____, R ____
- HCT: X ____, R ____
- MCV: X ____, R ____
- MCH: X ____, R ____
- MCHC: X ____, R ____
- PLT: X ____, R ____
.ccoomm
aaiill.
g mm
Current Calibration Coefficients
g
-
ed
ed@@
Write down the calibration coefficients as follows:
WBC: ____ (Venous blood mode), ____ (Capillary blood mode)
-
n g
g m
m
RBC: ____ (Venous blood mode), ____ (Capillary blood mode)
n
u o
o
Cut here
- HGB: ____ (Venous blood mode), ____ (Capillary blood mode)

-
pphh u
HCT: ____ (Venous blood mode), ____ (Capillary blood mode)
n
-
uyyee n
PLT: ____ (Venous blood mode), ____ (Capillary blood mode)
g
nng
-
-u NE%: ____ (Venous blood mode)
LY%: ____ (Venous blood mode)
- MO%: ____ (Venous blood mode)
- EO%: ____ (Venous blood mode)
- BA%: ____ (Venous blood mode)
- RDW: ____ (Venous blood mode)
- MPV: ____ (Venous blood mode)
New Calibration Coefficients

Write down the lot number of the MEK-3DN hematology control, new calibration coefficients and measured
data as follows:
Lot No. of MEK-3DN: _____________
Venous blood Capillary blood

Parameter Measured data Calibration coefficient Measured data Calibration coefficient
WBC
RBC
HGB
HCT
PLT
NE%
LY%
MO%
EO%
BA%
RDW
MPV

6. MAINTENANCE
Software Version
Write down the software version on the OPERATION HISTORY screen:
Software version: ___________
Operations
Touch screen function is checked. Yes No
Date and time settings are checked. Yes No
Automatic cleaning operations are normal during this maintenance check. Yes No
There is no fluid leakage on the hematology analyzer. Yes No
Constant volume of the diluent is properly dispensed when pressing the Dispense/Open key
on the front panel in capillary blood mode. Yes No
Cap pierce unit properly works when the cap pierce unit is installed
and the Open mode or Closed mode is selected. Yes No
Built-in Printer Unit Operation (When the printer is installed)
.cc omm
Pressing the Feed key on the front panel feeds the recording paper and there is no dot missing
o
aaiill.
on the paper by pressing the Print key on the front panel. Yes No
g mm
External Printer Unit Operation (When the external printer is connected)
g
ed
ed@@
Paper feed operation properly works on the external printer and there is no dot missing on the paper.
Yes No
n
n g
g m
m
u o
o
Cut here
Bar Code Reader Operation (When the bar code reader is installed)
pphh u
Light emitter and detector parts are cleaned with a cotton swab moistened with 80% alcohol.
n
uyyee n Yes No
g
nng u
Bar code reading operation properly works. Yes No
Others
Write down any other points on this sheet.

Section 7 Test point, Variable
Resister, LED and
Switch on Board
.cc omm
PRE AMP board .................................................................................................................. 7.1
o
aa ill.
HGB AMP board ................................................................................................................. 7.2
i
MANOMETER board ........................................................................................................... 7.2
ggmm
MV CONNECTION board .................................................................................................... 7.3
d @@
4WAY LIQUID SENSOR board ............................................................................................ 7.4
eed
n g
g m
SHEATH board .................................................................................................................... 7.5
m
TRIPLE PUMP board .......................................................................................................... 7.6
n
u o
o
DILUTER DRIVER board ..................................................................................................... 7.7
hh u
eennpp
SAMPLER-X board ............................................................................................................. 7.8
g u
uyy
SAMPLER-Z board .............................................................................................................. 7.8
KEY DISPLAY board ........................................................................................................... 7.9
nng VOLUME board ................................................................................................................... 7.9
POWER board ................................................................................................................... 7.10
AMP CONTROL board ...................................................................................................... 7.12
DRIVER board ................................................................................................................... 7.14

7. Test Point, Variable Resistor, LED and Switch on Board
PRE AMP board
SW011
SW012
TP022, TP021, TP011
.ccoomm
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ggmmVR021 D023, D024, D025, D026, D027, D028
ed
ed@@
n
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g m
TP011[EA] : Ground terminal for analog circuit
m
TP021[C ADJ] : Test point for circuit check operation
hhuuo
o TP022[CIN] : Test point for blood cell pulse operation
eennpp VR021[ELE VR] : Variable resistor for adjustment of voltage between the
g u
uyy electrodes
nng D023[HGB LDE] : LED for operation check of the HGB LED. When the HGB LED
is lit, this LED is also lit.
D024[WBC AMP] : LED for operation check of WBC measurement circuit
selection. When the circuit is selected, this LED is lit.
D025[WBC VOLT] : LED for operation check of WBC high voltage circuit
D026[RBC VOLT] : LED for operation check of RBC high voltage circuit
D027[RBC AMP] : LED for operation check of RBC measurement circuit selection.
When the circuit is selected, this LED is lit.
D028[CAL ON] : LED for operation check of circuit check pulse selection. When
the circuit check pulse is selected, this LED is lit.
SW011[MC-ADJ.1] : Switch for compensation of the manometer. This switch is
used for the second digit of the two-digit compensation value.
SW012[MC-ADJ.2] : Switch for compensation of the manometer. This switch is
used for the first digit of the two-digit compensation value.

HGB AMP board
TP052
TP051 VR051
TP051[EA] : Ground terminal for analog circuit.

TP052[HANA] : Test point for output voltage from HGB sensor.
VR051[HGB VR] : Variable resistor for adjustment of output voltage from HGB
sensor
.ccoomm
aaiill.
MANOMETER board
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp D041
g u
uyy
nng VR041
VR042
D042
TP041
TP042
TP043
TP041[UPE] : Test point for output voltage from the optical sensor at the upper
part of the manometer
TP042[LOWE] : Test point for output voltage from the optical sensor at the lower
part of the manometer
TP043[ED] : Ground terminal for digital circuit
VR041[UP VR] : Variable resistor for adjustment of output voltage from optical
sensor at the upper part of the manometer
VR042[LOW VR] : Variable resistor for adjustment of output voltage from optical
sensor at the lower part of the manometer
D041[UPER] : LED for operation check of optical sensor at the upper part of the
manometer. When the fluid level is higher than the upper sensor, this LED is lit.
D042[LOWER] : LED for operation check of optical sensor at the lower part of the
manometer. When the fluid level is higher than the lower sensor, this LED is lit.

MV CONNECTION board
MV8,MV9
MV1, MV2, MV3, MV4
MV5, MV6, MV7
D061[MV1] : LED for operation check of MV1 valve. When the MV1 works, this
LED lights.
LED lights.
.c oomm
c
LED lights.
aaiill.
ggmm
edd@
LED lights.
@
e
g m
m
LED lights.
n
n g
hhuuo
o D066[MV6] : LED for operation check of MV6 valve. When the MV6 works, this
LED lights.
eennpp D067[MV7] : LED for operation check of MV7 valve. When the MV7 works, this
g u
uyy LED lights.
nng D068[MV8] : LED for operation check of MV8 valve. When the MV8 works, this
LED lights.
LED lights.

4WAY LIQUID SENSOR board
TP011 VR011
TP012 VR012
TP013 VR013
TP014 VR014
TP015
TP015
TP016
.ccoomm
aaiill.
TP011[LIQ0] : Test point for output voltage from sensor for diluent and detergent
ggmm
TP012[LIQ1] : Test point for output voltage from sensor for hemolysing reagent
HEMOLYNAC5
edd@@
TP013[LIQ2] : Test point for output voltage from sensor for hemolysing reagent
e
n
n g
g m
m
HEMOLYNAC3
hhuuo
o
TP014[LIQ3] : Test point for output voltage from sensor in the sheath unit.
TP015[+5V] : +5 V DC terminal for digital circuit.
eennpp TP016[ED] : Ground terminal for digital circuit.
g u
uyy VR011[LIQ0] : Variable resistor for adjustment of output voltage from sensor for
nng diluent and detergent.

VR012[LIQ1] : Variable resistor for adjustment of output voltage from sensor for
HEMOLYNAC5 reagent
VR013[LIQ2] : Variable resistor for adjustment of output voltage from sensor for
HEMOLYNAC3 reagent
VR014[LIQ3] : Variable resistor for adjustment of output voltage from sensor in
the sheath unit.

SHEATH board
VR011 VR012
TP012
TP013
TP014
TP011
TP022
TP021
TP023
SW021
.ccoomm
aaiill.
ggmm
ed
ed@@ D022, D023
n
n g
g m
m
hhuuo
o TP011[EA] : Ground terminal for analog circuit
eennpp
TP012[PRESSURE] : Test point for output voltage from pressure sensor.
TP013[+15V] : Test point for +15 V DC for analog circuit.
g u
uyy TP014[-15V ] : Test point for -15 V DC for analog circuit.
nng TP021[TEMP_MIX] : Test point for temperature inside the mix chamber.
TP022[HEAT_STATUS] : Test point for heater operation
TP023[TEMP_BOTTOM] : Test point for environmental temperature at the bottom
of the instrument.
VR011[GAIN] : Variable resistor for gain adjustment of pressure sensor.
VR012[OFF SET] : Variable resistor for offset adjustment of pressure sensor.
D021[HEATER] : LED for operation check of the heater. When the heater works,
this LED lights.
D022[BOTTOM HOT] : LED for operation check in high environmental
temperature. When the temperature control works, the LED lights.
SW021 : Switch for temperature setting. See the following table
Bit switch setting Bit 1

Combination OFF ON
Default: Temperature in the mix chamber is
When the environmental temperature fixed to 36.3°C.
is 15 to 23°C, temperature in the mix
OFF chamber is set to 36.3°C.
When the environmental temperature
is 23°C or higher, temperature in the
Bit 2 mix chamber is set to 30°C.
When the environmental temperature Temperature in the mix chamber is
is 15 to 23°C, temperature in the mix fixed to 34.1°C.
chamber is set to 34.1°C.
ON
When the environmental temperature
is 23 to 28°C, temperature in the mix
chamber is set to 28°C.

TRIPLE PUMP board
D014, D015, D016, D017, D018, D011, D012, Do13
.ccoomm
aa ill.
D011[HCPHI] : LED for operation check of the high position sensor. When the
i
sensor is turned on, this LED is lit.
ggmm
D012[HCPMID] : LED for operation check of the middle position sensor. When the
d @@
eed
n
n g
g m
D013[HCPLOW] : LED for operation check of the low position sensor. When the
m
hhuuo
o D014[MV1] : LED for operation check of the MV1 valve. When the valve is turned
eennpp on, this LED is lit.
g u
uyy
D015[MV2] : LED for operation check of the MV2 valve. When the valve is turned
nng
on, this LED is lit.

DILUTER DRIVER board
D0112
D0111
SW012
SW011
TP012
D0110
TP011
D019
TP013 TP014
TP011[ORIGIN] : Test point for output voltage from the home position sensor for
.ccoom
the piston of the diluter.
m
aaiill.
TP012[ENCODE] : Test point for output voltage from the position encoder for the
ggmm
piston of the diluter.
ed
ed@@
TP014[+5V] : Test point for +5 V DC for digital circuit
n
n g
g m
m
SW011[DIL.ADJ.2] : Switch for compensation of the diluter. This switch is used for
the first digit of the two-digit compensation value.
hhuuo
o SW012[DIL.ADJ.1] : Switch for compensation of the diluter. This switch is used for
eennpp the second digit of the two-digit compensation value
g u
uyy D019[ENCODE] : LED for operation check of the position encoder for the piston
nng of the diluter. When the encoder is turned on, this LED is lit.
D0110[ORIGIN] : LED for operation check of the home position sensor for the
piston of the diluter. When the sensor is turned on, this LED in lit.
D0111[MD1MV] : LED for operation check of the MV1 valve. When the valve is
turned on, this LED is lit.
D0112[MD2MV] : Reserved. (LED for operation check of the MV2 valve. When
the valve is turned on, this LED is lit.)

SAMPLER-X board
D011 D012 D013

D014
D015 D017 D024
D016 D025
.ccoomm
aaiill.
D011[STB] : LED for operation check of standby position sensor on the X-axis.
When the sensor is turned on, the LED is lit.
ggmm
D012[SEN1] : LED for operation check of the position sensor 1 on the X-axis.
ed
ed@@
n
n g
g m
D013[SEN2] : LED for operation check of the position sensor 2 on the X-axis.
m
hhuuo
o D014[HIGH] : LED for operation check of the high position. When the sensor is
eennpp turned on, this LED is lit.
g u
uyy D015[MID] : LED for operation check of the middle position. When the sensor is
nng turned on, this LED is lit.

D016[LOW] : LED for operation check of the low position. When the sensor is
turned on, this LED is lit.
D017[MSMV] : LED for operation check of the valve. When the valve is turned
D024[MFLENCD] : Reserved (LED for operation check of the position encorder
for MA-720V flow cell adjuster. When the encoder is turned on, this LED is lit.)
D025[MFLORG] : LED for operation check of the original position sensor for MA-
720V flow cell adjuster. When the sensor is turned on, this LED is lit.
SAMPLER-Z board
There is no test point

KEY DISPLAY board
VR011 (UT-7168) UT-7167 D018
D011
SW011
SW012
D012
D013
D014
D015
.ccoomm
aaiill. SW015, SW014, SW013
D017
D019
ggmm
d @@
D011[BIRYOU] : LED for capillary mode operation. When the instrument is in the
eed
n g
g m
capillary mode, this LED is lit.
m
D012[SAMPLE 0] : LED for operation indication. When the instrument is in
n
hhuuo
o standby mode, this LED is lit.
eennpp D013[SAMPLE 1] : LED for operation indication. When the instrument is in
g u
uyy
standby mode, this LED is lit.
nng standby mode, this LED is lit.
standby mode, this LED is lit.
D017[MAIN POWER] : LED for status indication of the main power switch. When
the main power switch is set to on, this LED is lit.
D018[SUB POWER] : LED for status indication of the Power key When the Power
key is pressed, this LED is lit.
D019[LASER KEY] : LED for status indication of the laser switch. When the laser
switch is set to on, this LED is lit.
SW011[BIRYOU] : Capillary mode switch
SW012[TOSYUTU] : Diluent dispense switch in capillary mode.
SW013[POWER] : Power key
SW014[RESET] : Reset key
SW015[CLEAN] : Clean key
VOLUME board
VR012[OFF SET] : Variable resistor for offset adjustment of pressure sensor.

POWER board
D017
TP0117 D0113 D0110
TP0114
TP0113, TP0112
TP0115
TP0118
SW022
TP0116 D0111, TP019, TP0110
SW021
TP0111
TP014
D0114
TP015
.ccoomm
TP016
aaiill.
ggmm TP011
ed
ed@@ TP013
TP012
n
n g
g m
m
hhuuo
o
eennpp TP011[+27V] : +27 V DC terminal for LCD
g u
uyy TP013[-36V] : -36 V DC terminal for analog circuit.
nng TP014[+15V] : +15 V DC terminal for analog circuit.

TP015[EA] : Ground terminal for analog circuit.
TP016[-15V] : -15 V DC terminal for analog circuit
TP017[+A12V] : +12 V DC for analog circuit
TP018[EA12] : Ground terminal for +12 V DC use analog circuit
TP019[Vu] : Supply voltage (Vu) terminal for motor control
TP0110[+12V] : +12 V DC terminal for valve control
TP0112[+5V] : +5 V DC terminal for digital circuit
TP0113[+3.3V] : +3.3 V DC terminal for digital circuit
TP0115[Vcc] : Supply voltage (Vcc) terminal for secondary power circuit control
TP0116[Vp] : Supply voltage (Vp) terminal for optional built-in type printer unit.
TP0117[Ep] : Ground terminal for optional built-in type printer unit.
TP0118[E12] : Ground terminal for valve control
TP0111[Eu] : Ground terminal for motor control
TP021[POWER ON] : Test point for power off control signal
D017[Vcc] : LED for supply voltage check for secondary power circuit control.
When the voltage is supplied to the circuit, this LED is lit.
D0110[+5V] : Test point for +5 V DC for digital circuit. When the +5 V DC is
supplied to the digital circuit, this LED is lit.
D0111[Vu] : LED for check of supply voltage for motor control. When the voltage
is supplied to the motor control circuit, this LED is lit.

D0112[+15V] : LED for check of +15 V DC for analog circuit. When the +15 V DC
is supplied to the analog circuit, this LED is lit.
D0113[+A12V] : LED for check of +12 V DC for analog circuit. When the voltage
is supplied to the analog circuit, this LED is lit.
D0114[-36V] : LED for check of -36 V DC for analog circuit. When the voltage is
supplied to the analog circuit, this LED is lit.
SW021[POEWR] : Power switch
SW022 : Bit switches for power supply mode selection. See the following table.
Bit switch Default Operation when the bit switch is set to ON.
No. 1 OFF When the main power switch is turned on, only +5 V DC is live.
No. 2 OFF When the main power switch is turned on, the -36 V, ±15 V, +12 V,
+A12 V, +27 V, Vu and Vp supply voltages are live.
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng

AMP CONTROL board
TP017
TP018
TP0112
TP0115
TP0114, TP0113, TP0111
TP013, TP014, TP015, TP016

TP0110
TP011, TP012, TP019
SW061
D178
.ccoomm
TP113, TP114
aaiill.
TP111, TP112
ggmm
ed
ed@@
D251
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy TP076, TP075
nng
TP073
TP074
TP071, TP081, TP091, TP061
TP072, TP082. TP092
TP077, TP078, TP084, TP083
TP094
TP093
TP021 TP022
TP033
TP034
TP023
TP031
TP024
TP032
TP079 TP103, TP102

TP101
TP011[+5V] : +5 V DC terminal for digital circuit

TP015[+A12V] : +12 V DC terminal for analog circuit
TP016[EA12] : Ground terminal for +12 V DC supplied to analog circuit

TP017[Vp] : Supply voltage (Vp) terminal for optional built-in printer
TP018[Ep] : Ground terminal for optional built-in printer
TP019[Vcc] : Supply voltage (Vcc) for secondary power circuit control
TP0110[+3.3V] : +3.3 V DC terminal for digital circuit
TP0112[+27V] : +27 V DC terminal for LCD
TP0114[EA] : Ground terminal for analog circuit
TP0115[+15V] : +15 V DC termial for analog circuit
TP021[CIN] : Test point for blood cell pulses from MC-720V CBC measuring unit
TP022[CFIL] : Test point for output waveform through the low-pass filter
TP023[RB] : Test point for output waveform from the Robinson gate circuit
TP024[CLOUT] : Test point for output waveform from baseline stabilizer circuit
TP031[PEAK HOLD] : Test point for output waveform from the peak-hold circuit
.ccoom
TP032[CANA] : Test point for output waveform from the gain setting circuit
m
TP033[LOW THR] : Test point for lower threshold level
aaiill.
TP034[HIGH THR] : Test point for upper threshold level
g mm
TP061[+VLD] : Test point for laser operation
g
eed@@
TP071[ANA1] : Test point for output waveform for FS (channel 1) from MO-820V
d
laser optical unit
n g
g m
m
TP072[FIL1] : Test point for output waveform through the low-pass filter for FS
n
hhuuo
o (channel 1)
eennpp TP073[GATE] : Test point for output waveform from the gate circuit for 5-part
g u
uyy
differential WBC
TP074[RBP] : Test point for output waveform of Robinson gate pulse for 5-part
nng differential WBC

TP075[RB1] : Test point for output waveform from the Robinson gate circuit for 5-
part differential WBC
TP076[RBTHR] : Test point for threshold level on the Robinson gate circuit for 5-
part differential WBC
TP077[PHA1] : Test point for output waveform from the peak-hold circuit for FS
(channel 1)
TP078[CL1] : Test point for output waveform from the baseline stabilizer circuit
for FS (channel 1)
TP079[PEAK-HOLD] : Test point for the peak-hold control signal for 5-part
differential WBC
TP081[ANA2] : Test point for output waveform for FL (channel 2) from MO-820V
laser optical unit
TP082[FIL2] : Test point for output waveform through the low-pass filter for FL
(channel 2)
for FL (channel 2)
TP084[PHA2] : Test point for output waveform from the peak-hold circuit for FL
(channel 2)
TP091[ANA3] : Test point for output waveform for SD (channel 3) from MO-820V
laser optical unit
TP092[FIL3] : Test point for output waveform through the low-pass filter for SD
(channel 3)

for SD (channel 3)
TP094[PHA3] : Test point for output waveform from the peak-hold circuit for SD
(channel 3)
TP101[PKD] : Test point for peak detection pulse of 5-part differential WBC
TP102[ANA1-THL] : Test point for threshold level of 5-part differential WBC
TP103[THP] : Test point for threshold pulse of 5-part differential WBC
TP111 : Test point for operation check of 5-part differential WBC (A/D BUSY)
TP112 : Test point for operation check of 5-part differential WBC (IRQ1CK)
TP113 : Test point for operation check of 5-part differential WBC (IRQ1RS)
TP114 : Test point for operation check of 5-part differential WBC (IRQ1EN)
D178[POWER] : LED for status indication of power supply to this board. When the
power is supplied to the board, this LED is lit.
D251[CARD IN] : LED for status indication of memory card insertion. When the
card is inserted into the slot of the instrument, this LED is lit.
.ccoom
SW061[RESET] : Reset switch for this board
m
aaiill.
DRIVER board ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o D051 TP018
eennpp D052 TP017
g uuyy D091 TP016
nng D092
D093
D094
TP015
TP014
TP013
D095 TP012
D096 TP011
D097
D098
TP093
D099
TP092
D0910
TP091
D0911
D0912
D0913
D0914
D0915
D0916
D0917
D0918
D0919
D0920
D0921
D087
D088
D061
D062

TP011[+5V] : +5V DC terminal for digital circuit

TP015[+A12V] : +12 V DC terminal for analog circuit
TP016[EA12] : Ground terminal for +12 V DC (+A12V) supplied analog circuit
TP017[Vu] : Supply voltage (Vu) for motor control
TP018[Eu] : Ground terminal for motor control
TP091 : Test point for protection to a wrong connector connection
D051[RS] : LED for operation check of the RBC sub bath in the MC-720V CBC
measuring unit. When the RBC sub bath works, this LED lights.
D052[WS] : LED for operation check of the WBC sub bath in the MC-720V CBC
measuring unit. When the WBC sub bath works, this LED lights.
.ccoom
D061[P2] : LED for operation check of sensor 2 in the MP-520V pump unit. When
m
the sensor is turned on, this LED is lit.
aaiill.
D062[P1] : LED for operation check of sensor 1 in the MP-520V pump unit. When
g mm
the sensor is turned on, this LED is lit.
g
eed@@
D087[C1] : LED for operation check of valve 1 in the MS-721V cap pierce unit.
d
When the valve is turned on, this LED is lit.
n g
g m
m
D088[C2] : LED for operation check of valve 2 in the MS-721V cap pierce unit.
n
hhuuo
o When the valve is turned on, this LED is lit.
eennpp D091[1] : LED for operation check of valve 1 in the MJ-721V sheath unit. When
g u
uyy
the valve is turned on, this LED is lit.
D092[2] : LED for operation check of valve 2 in the MJ-721V sheath unit. When
nng the valve is turned on, this LED is lit.

D0910[10] : LED for operation check of valve 10 in the MJ-721V sheath unit.

D0918[E1] : LED for operation check of spare valve 1. When the valve is turned
D0919[J3] : LED for operation check of valve 3 in the MJ-720V inlet/outlet unit.
D0920[J4] : LED for operation check of valve 4 in the MJ-720V inlet/outlet unit.
D0921[E2] : LED for operation check of spare valve 2. When the valve is turned
.ccoomm
aaiill.
ggmm
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng

Section 8 Demonstration Guide
.cc omm
Introduction ......................................................................................................................... 8.1
o
aa ill.
Demonstration Outline ............................................................................................... 8.1
i
Required Items for Demonstration ............................................................................. 8.2
ggmm
Preparing the Hematology Analyzer .................................................................................... 8.4
d @@
Installation Flowchart ................................................................................................ 8.4
eed
n g
g m
Connecting Tubes ...................................................................................................... 8.4
m
Connecting the Power Cord and the Grounding the Hematology Analyzer ................. 8.6
n
hhuuo
o Turning the Laser Switch On ..................................................................................... 8.6
eennpp Turning On the Power ................................................................................................ 8.7
g u
uyy
Setting the Date & Time and Cleaning the Hematology Analyzer ........................................ 8.8
Checking the Date and Time Settings ....................................................................... 8.8
nng Cleaning the Fluid Path in the Hematology Analyzer ................................................. 8.8
Adjusting Gain and Measuring Background Noise ............................................................... 8.9
Flowchart .................................................................................................................. 8.9
Reference: Principle of Differentiating WBC .............................................................. 8.9
Counting the Polymer Microsphere Suspensions .................................................... 8.10
Adjusting the Flow Cell Unit Position ....................................................................... 8.12
Adjusting Gain (FS and FL) for WBC 5 Part Differential Parameters in Rough Mode
Using Polymer Microsphere Suspensions ............................................................... 8.15
Measuring Background Noise .................................................................................. 8.16
Adjusting Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous
Blood Samples ........................................................................................................ 8.17
Checking the Gain Adjustment ................................................................................ 8.20
Calibration ......................................................................................................................... 8.21
Procedure Flowchart ............................................................................................... 8.21
Calibration for CBC Paramters with Calibrator ......................................................... 8.21
Calibration for WBC 5 Part Differential Parameters .................................................. 8.26
Checking Data ................................................................................................................... 8.27
Checking Data with MEK-5D Hematology Control ................................................... 8.27
Checking Data with Human Blood Samples ............................................................ 8.28
Interference Substances ................................................................................................... 8.29
Storing and Transporting the Hematology Analyzer ........................................................... 8.32
MEK-7222J/K Hematology Analyzer Installation Check Sheet .......................................... 8.34

8. DEMONSTRATION GUIDE
Introduction
This demonstration guide provides an installation and setup flow chart for a
demonstration of the MEK-7222J/K hematology analyzer.
The installation takes approximately 2 hours. Copy the check sheet provided at
the end of this manual and use it for checks. It is also useful in troubleshooting.
Demonstration Outline Do the following steps to install and set up the hematology analyzer for
demonstration.
.c oomm
1. Prepare the hematology analyzer.
c
a iill.
a) Connect the tubes.
a
ggmm
b) Connect the power cord and ground the hematology analyzer.
ed
ed@
c) Turn the laser switch on.
@
d) Turn on the power.
n
n g
g m
m
hhuuo
o2. Set the settings and clean the fluid path in the hematology analyzer.
eennpp
a) Check and set the date and time settings.
b) Clean the fluid path in the hematology analyzer by pressing the clean key
g u
uyy on the front panel.
nng 3. Adjust the gain and measure background noise

a) Count the polymer microsphere suspensions to check the CV and PEAK
values.
b) Adjust the flow cell unit position.
c) Adjust the gain (FS and FL) for WBC 5 part differential parameters in rough
mode using polymer microsphere suspensions.
d) Measure background noise.
e) Adjust gain for WBC 5 part differential parameters in fine mode using
venous blood samples from different healthy people which are 30 minutes
to 8 hours after collection.
f) Check the actual peak list for all samples to see if the gain is adjusted
appropriately. Make sure that the average peak of MO is optimum.
4. Calibrate the hematology analyzer.

a) Perform calibration measurement with MEK-3DN calibrator to obtain a
mean value for calculating the new calibration coefficient. The MEK-3DN
calibrator can be used as a calibrator if it is stored at 2 to 8°C and is used
within 3 days after opening. To ensure higher accuracy, use the calibrator
soon after opening.
b) Perform the calibration for CBC parameters (WBC, RBC, HGB, MCV, PLT,
RDW, MPV).
c) Check that the calibration coefficients for WBC 5 part differential
parameters (LY%, MO%, EO%, BA%) are 1,000 on the CALIBRATION
screen. In the gain adjustment performed in step 3, WBC 5 part differential

parameters are already accurately calibrated. Therefore, you only need to
check the calibration coefficient.
5. Check the following data.

a) Measure the MEK-5DN/H/L hematology control and check that the
obtained data falls within the acceptable range on the assay sheet attached
to the hematology control.
b) Count more than 10 venous blood samples of different healthy people
which are 30 minutes to 8 hours after collection and make sure that there is
no flag such as “Left Shift”, “Eosinophilia”, “Blasts”, “Immature
Granulocyte”, or “Atypical Lymphocytes” displayed.
Required Items for

.ccoomm
Make sure that you have the following necessary items for the demonstration.
Demonstration
aaiill.
These are provided with the hematology analyzer. The number in the parentheses
g mm
indicates the necessary quantity.
g
ed
ed@@
n
n g
g m
m
hhuuo
o
eennpp
Power cord (1)
g u
uyy Ground lead (1) Waste tube (1), CLEANAC tube (1), 18 L cap (3)
nng ISOTONAC tube (1)
18 L diluent tube assy (1) 18 L diluent tube assy Cleanac tube 8 for Cleanac tube assy
8 WASTE (1) CLEANAC•3 (1) 8222 (2)
Hemolynac3 cap (2) Hemolynac•3 tube assy (1) Hemolynac•5 tube assy (1) Waste container (1)
Laser key (2) Manuals (1 set)

The following items are also required.
• Diluent ISOTONAC•3, MEK-640

• Detergent CLEANAC, MEK-520
• Detergent CLEANAC•3, MEK-620
• Hemolysing reagent HEMOLYNAC•3, MEK-660, or HEMOLYNAC•3N, MEK-
680
• Hemolysing reagent HEMOLYNAC•5, MEK-910
• 7 µm polymer microsphere suspensions, YZ-0194 (supply code no. T905)
• Hematology control, MEK-3DN
• Hematology control, MEK-5DN/H/L
• Sample containers
• Wiping cloth (tissue papers)
• Rubber or latex gloves
• Distilled water
•
•
.cc om
Cleaning bottle kit, YZ-0252
o m
15 blood samples from different healthy people
aaiill.
ggmm
ed
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n
n g
g m
m
hhuuo
o
eennpp
g u
uyy
nng

Preparing the Hematology Analyzer
Installation Flowchart 1. Connect the diluent, detergent, hemolysing reagent and waste containers to
2. Connect the power cord and perform grounding, if necessary.
3. Turn the laser switch on the right side panel to on for WBC 5 part differential
measurement.
4. Turn on the power of the hematology analyzer.
.ccoomm
Connecting Tubes
a iill.
Connect the following reagent bottles and waste container.
a
• Waste container
ggmm
ed
ed@
• Diluent ISOTONAC•3
@
• Detergent CLEANAC•3
g m
m
• Detergent CLEANAC
n
n g
hhuuo
o
• Hemolysing reagent HEMOLYNAC•3 or HEMOLYNAC•3N
eennpp
• Hemolysing reagent HEMOLYNAC•5
g u
uyy Tubes and caps necessary for connecting the reagents and container and the inlets
nng and outlets to which these reagents and container are to be connected are indicated
in the following illustration and table. For details, refer to the operator’s manual.
CAUTION
Avoid reagents or waste fluid contact with the skin. If it contacts the skin
or eyes or is swallowed, wash thoroughly with water and see a
physician immediately.
NOTE
• Be careful not to let dust get in the hemolysing reagent, diluent or
detergent.
• When using the diluent container, detergent container or waste
container, follow the instructions on each package.
• Place the diluent and detergent containers at the same level as the
• Do not squeeze or bend the tubes. Otherwise the hematology analyzer
may be damaged.
• If necessary, cut the tube to an appropriate length if it does not fit.
• Only use the specified detergent tubes for the detergent.
• When using HEMOLYNAC•3N reagent after using HEMOLYNAC•3
reagent, calibrate the hematology analyzer after changing the reagent.
Otherwise the hemoglobin concentration decreases.
• When using HEMOLYNAC•3N reagent, attach the HEMO3N label

provided with the hematology analyzer to cover the HEMO3 label at
the HEMO3 inlet.
Refer to “Connecting tubes”

in section 2 of the MEK-
7222 operator’s manual.
5
4 3 1
2
.ccoomm Inlet/Outlet on the

Reagent/Container Cap
aaiill. Tube/Tube ASSY

18 L diluent tube assy ISOTONAC
hematology analyzer
ggmm
18 L cap tube ISO3 Inlet
1 Diluent ISOTONAC•3
ed
ed@@ (A)
n
n g
g m
m Cleanac tube assy CLEANAC tube
2
h
Detergent CLEANAC
huuo
o (8222) CLN Inlet
(B)
eennpp
g u
uyy Cleanac tube 8
(A)
3
nng
Detergent
CLEANAC•3
CLN3
Inlet (C)
(B)
(C)
(D)
(E)
Hemolysing reagent Hemolynac3 tube assy
Hemolynac3 HEMO3
4 HEMOLYNAC•3 or (F)
cap Inlet (D)
HEMOLYNAC•3N
Hemolynac5 tube assy
Hemolysing reagent HEMO5
5
HEMOLYNAC•5 Inlet (E)
18 L tube assy 8 Waste tube

Attached to (WASTE) WASTE
6 Waste fluid container
the container Outlet (F)

Connecting the Power Connecting the Power Cord

Cord and Grounding the Connect the provided power cord to the AC SOURCE socket on the rear panel and
Hematology Analyzer plug the cord into a 3-prong AC outlet on the wall.
CAUTION
Only use the provided power cord. Using other power cords may result
in electrical shock or other injury to the operator.
Equipotential ground terminal
AC SOURCE socket Equipotential Grounding

When equipotential grounding is required, connect the equipotential ground
terminal on the rear panel of the hematology analyzer (terminal marked by ) to
the equipotential ground terminal on the wall (equipotential grounding system)
.ccoomm
with the equipotential grounding lead (potential equalization conductor).
aaiill.
ggmm WARNING
d @@
For operator safety, equipotential grounding of all instruments must be
eed
n
n g
g m
performed. Consult with a qualified biomedical engineer.
m
hhuuo
o
eennpp
g u
uyy
On
nng
Turning the Laser Switch Insert the laser key into the laser switch hole on the right side panel and turn the
laser switch to the side marked by .
NOTE
• When the measurement is performed with the laser switch turned off,
CBC can be measured but there is no WBC 5 part differential
scattergram and data, and an alarm occurs.
• Store the key in a safe place.

Turning On the Power 1. Press the main power switch on the rear panel of the hematology analyzer to
on. The main power lamp on the front panel lights.
Main power switch
2. Press the power key on the front panel to on. The power lamp lights. When
the laser switch on the right side panel is turned on, the laser lamp also lights.
Main power lamp Checking the reagents, priming and circuit self-check are automatically
Power lamp
.cc om
performed. After priming operation is completed, the READY screen
o m
appears. The hematology analyzer is ready for counting.
aaiill.
ggmm
Laser lamp
Power key
ed
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m
hhuuo
o
eennpp
g u
uyy
nng

Setting the Date & Time and Cleaning the Hematology Analyzer
Checking the Date and After turning the hematology analyzer power on, check that the date and time
Time Settings displayed at the upper right of the screen are correct.
1. Display the DATE & TIME screen.

MENU → SETTINGS → DATE & TIME
2. Select the setting item by pressing the item key. The cursor moves to the item.
Item keys
.ccoomm
aaiill. Numeric keypad
ggmm
ed
ed@@
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m
hhuuo
o
3. Enter the value using the numeric keys. The entered value appears next to
eennpp VALUE.
g u
uyy 4. Press the ENTER key to register the value. The cursor moves to the next item.
nng 5. Repeat steps 2 to 4 to enter other items.
6. Press the OK key to finish setting and return to the SETTINGS screen. The
clock starts immediately from the new date and time.
Cleaning the Fluid Path in 1. Clean the fluid path in the hematology analyzer by pressing the clean key ( )
the Hematology Analyzer on the front panel.
Clean key
The READY screen appears after cleaning. It takes about 12 minutes for
cleaning.
2. Perform cleaning again by pressing the Clean key.

Clean the fluid path more than 2 times.
Adjusting Gain and Measuring Background Noise
Flowchart 1. Count the polymer microsphere suspensions to check the CV and PEAK
values.
2. Adjust the flow cell unit position.
3. Adjust gain for WBC 5 part differential parameters in rough mode using
polymer microsphere suspensions.
4. Measure background noise.
5. Adjust gain for WBC 5 part differential parameters in fine mode using venous
c oom
blood samples from different healthy people which are 30 minutes to 8 hours
m
after collection.
. c
aaiill.
ggmm
6. Check the actual peak list for all samples. Make sure the average peak of MO
ed
ed@@
is optimum.
n
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g m
m
hhuuo
o
e nnpp
Reference: Principle of
e The hematology analyzer uses the light scatter
g u
uyy
Differentiating WBC technique to differentiate WBC into neutrophil,
nng lymphocyte, monocyte, eosinophil and basophil counts.
The diluted blood sample is injected into the flow cell.

Blood cells pass through the sensing zone one by one.
A laser beam through the sensing zone is scattered by
Granularity (SD) Complexity (FL)
Laser the passing cells and the scattered light is detected. The
angle and intensity of scattered light indicates the
Size (FS) volume and characteristics of the cell. From this, WBC
is differentiated into 5 parts.
In gain adjustment, you adjust the following.

• FS (forward small angle scatter)
• FL (forward large angle scatter)
• SD (side scatter)
From the forward small angle scatter, the size of the cell
Diluent Diluent is detected.
Sample From the forward large angle scatter, the complexity of
the cell is detected.
From the side scatter, the granularity of the cell is
detected.

Counting the Polymer Count the polymer microsphere suspensions (YZ-0194, supply code no. T905) to
Microsphere Suspensions check the CV and PEAK values.
1. Clean the flow cell to remove dust and bubbles inside the flow cell. Press the
CLEAN FLOWCELL key on the OPERATIONS screen to clean the flow cell.
2. Prepare 1 mL of polymer microsphere suspensions in a sample container.
3. When the MS-721V cap pierce unit is installed, select OPEN mode on the
ORDER screen.
4. Display the PARTICLE TEST screen.
.ccoom
MENU → OPERATIONS → PARTICLE TEST
m
aaiill.
g mm
5. Press the MEASURE key. The “PRESS [COUNT] KEY TO START” message
g
ed
ed@@
appears and the hematology analyzer is ready for counting the polymer
microsphere suspensions.
n
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g m
m
hhuuo
o
eennpp
g u
uyy
nng 6. Put the sampling nozzle into the bottom of the sample container containing
comes near the bottom of the sample container.
Sampling nozzle
NOTE
Count switch Do not let the sampling nozzle touch the bottom of the sample
Put the sampling suspensions.
7. Press the Count switch. The polymer microsphere suspensions is aspirated and
measurement starts. The “MEASURING PARTICLES” message appears on the
screen and the scattergrams and histograms appear in real-time.
The measurement lasts approximately 40 seconds.
8. After finishing the particle test, the CV and PEAK values calculated from the
scattergram and histogram appear on the screen.

Scattergram FS histogram
FL histogram
SD histogram
Check the CV and PEAK of FS and FL
9. Check that CV and PEAK values are within the following range.
FS CV: 7.0% or less

PEAK: 61 ± 3
.ccoomm
FL CV: 7.0% or less
aaiill. PEAK: 90 ± 3
ggmm NOTE
edd@
• There is no need to check CV and PEAK values of SD.
@
• If the CV value of FS or FL is more than 7%, the flow cell position
e
n
n g m
m
must be adjusted. Refer to the “Adjusting the Flow Cell Unit Position”
g
hhuuo
o section.
• If the PEAK value of FS or FL is outside the above range, the gain for
eennpp WBC 5 part differential measurement must be adjusted. Press the
g u
uyy GAIN key to go to the SENS&THRESHOLD screen to adjust gain. Refer
nng to the “Adjusting Gain (FS and FL) for WBC 5 Part Differential
Parameters in Rough Mode Using Polymer Microsphere Suspensions”
section.
FS (Forward Small): Size (vertical axis of the scattergram)

FL (Forward Large): Complexity (horizontal axis)
SD (Side): Granularity (horizontal axis)
THR: Threshold of FS
When the CV is Not Optimum

• CV of FS or FL is over 7.0%
If the polymer microsphere suspension is old, it clots and CV value is not
optimum. Replace the polymer microsphere suspensions and perform the
particle test again.
The polymer
microsphere
suspensions clots.
• The scattergram is large

If dust or bubbles accumulate inside the flow cell, the scattergram of polymer
microsphere suspensions becomes large. Clean the flow cell by pressing the
CLEAN FLOWCELL key on the OPERATIONS screen or clean the fluid path in
the hematology analyzer by pressing the Clean key on the front panel. If this
does not improve it, adjust the flow cell position.
The scattergram is
large.
.ccoomm
aaiill.
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ed
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Adjusting the Flow Cell
g m
m
Check Points before Adjusting the Flow Cell Unit Position
n
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Unit Position
hhuuo
o
The flow cell unit position is adjusted to the most appropriate position at the
factory. When the CV value from the particle test is over the normal range, it is
eennpp probably caused by dust or bubbles inside the flow cell. Check the following
g u
uyy items before adjusting the flow cell unit position.
nng 1. Did you clean the fluid path and flow cell in the hematology analyzer by
pressing the Clean key on the front panel or by pressing the CLEAN
FLOWCELL key on the OPERATIONS screen?
You need to completely remove air bubbles from inside the flow cell with the
CLEANAC detergent when the hematology analyzer is first installed. Bubbles
inside the flow cell cannot be removed by priming at power on or strong
cleaning. Bubbles and dust can be removed by cleaning the flow cell by
pressing the Clean key on the front panel or pressing the CLEAN FLOWCELL
key on the OPERATIONS screen.
If the CV from the particle test is over the normal range, perform cleaning by
pressing the Clean key on the front panel or CLEAN FLOWCELL key on the
OPERATIONS screen.
2. CLEANAC and CLEANAC•3 detergents are connected correctly?
If CLEANAC and CLEANAC•3 detergents are connected incorrectly, bubbles

inside the flow cell can not be removed.
Connect CLEANAC and CLEANAC•3 correctly and clean the hematology
analyzer by pressing the Clean key on the front panel or CLEAN FLOWCELL
key on the OPERATIONS screen.

3. Out of diluent or detergent alarm are not displayed?
If diluent or detergent runs out, bubbles enter the flow cell. If the “out of
diluent or detergent” alarm appears, replace diluent or detergent and clean the
fluid path in the hematology analyzer by pressing the Clean key on the front
panel or CLEAN FLOWCELL key on the OPERATIONS screen.
4. The polymer microsphere suspensions are old or coagulated.
Perform the particle test after getting new polymer microsphere suspensions
and adjust the flow cell unit position only when the CV value of the particle
test greatly exceeds 7%.
Adjusting the Flow Cell Unit Position
.ccoom
The flow cell unit must be adjusted when the laser does not scatter exactly on the
m
sample flowing inside the flow cell. Check if the laser scatters on the sample by
aaiill.
the particle test.
ggmm
ed
ed@@ NOTE
• When the flow cell is clogged with dust, it cannot be adjusted
n
n g
g m
m correctly.
hhuuo
o • Clean the fluid path in the hematology analyzer by pressing the Clean
eennpp key on the front panel or CLEAN FLOWCELL key on the OPERATIONS
g u
uyy
screen before adjusting the flow cell unit position.
nng 1. Prepare 1 mL of polymer microsphere suspensions in a sample container.
2. Display the MANUAL ADJUSTMENT screen.

MENU → OPERATIONS → ADJUST FLOWCELL → MANUAL ADJUST
3. Press the MEAS key. The “PRESS [COUNT] KEY TO START” message
appears and the hematology analyzer is ready to count the polymer
microsphere suspensions. Use the CANCEL key to return to the OPERATIONS
screen.
MEAS key

4. Put the sampling nozzle into the bottom of the sample container containing
comes near but does not touch the bottom of the sample container.
Sampling nozzle
NOTE
Count switch Do not let the sampling nozzle touch the bottom of the sample
Put the sampling suspensions.
5. Press the Count switch. The polymer microsphere suspensions in the sample
container are aspirated and measurement starts. The “MEASURING
PARTICLE” message appears on the screen and the scattergrams and
histograms appear in real-time.
The measurement takes approximately 40 seconds.
FS histogram
.ccoomm
aaiill. FL histogram
ggmm
and FL are displayed.
ed
CV and PEAK values of FS
ed@@
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n g
g m
m
hhuuo
o
eennpp
g u
uyy Adjust the flow cell
nng The histogram is redrawn without

adjusting the flow cell unit.
unit
6. Press the → or ← key to adjust the flow cell unit position. The histogram is
automatically redrawn. Adjust the flow cell unit until the CV of FS and FL is
minimum and the peak is maximum.
NOTE
• Check the CV and PEAK values by measuring the polymer
microsphere suspensions again after adjusting the flow cell unit.
• If the CV values obtained from measuring new polymer microsphere
suspensions which are not coagulated are not optimum after
adjusting the flow cell unit, the hematology analyzer may be damaged.
Contact your Nihon Kohden distributor.

Adjusting Gain (FS and FL) NOTE

for WBC 5 Part Differential Adjust the FS and FL gain only when the PEAK values are not optimum
Parameters in Rough Mode in the particle test.
Using Polymer
Microsphere Suspensions 1. Display the SENS & THRESHOLD screen for WBC 5 part differential
parameters by doing one of the following:
a) Press the GAIN key on the PARTICLE TEST screen.
b) Press the SENS & THRESHOLD key on the SETTINGS screen and press
the DIFF key.
NOW: Current peak Press this key to select

sample type from
AIM: Target peak PARTICLE, BLOOD and
CONTROL.
In PARTICLE mode, the

scattergram of the polymer The entered value
microsphere suspensions is displayed.
is displayed.
.ccoomm
aaiill.
ggmm
ed
ed@@
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m
uuo
o
Press OK key to return
hh
Register the entered
eennp
to the SETTINGS
p
screen.
value.
g u
uyy The appropriate setting gain
value is calculated from the peak
nng
In PARTICLE mode, press this
key to return to the PARTICLE NOW and AIM and the calculated
TEST screen. gain is displayed in the VALUE
box.
The 3 types of histogram (Particle, Blood and Control) and its PEAK value can
be displayed with the sample type key on the SENS & THRESHOLD screen for
Diff.
NOW PEAK is the current peak of the displaying scattergram and AIM is the
target peak. If the gain is adjusted to the most appropriate value, the values of
NOW and AIM match. If the difference between the NOW value and AIM
value is more than 5%, the gain must be adjusted.
NOTE
Check that the CV of FS and FL are optimum (less than 7.0%) by
counting the polymer microsphere suspensions before adjusting gain.
Refer to the “Cleaning the Fluid Path If the CV is not optimum, count the polymer microsphere suspensions
in the Hematology Analyzer”, again after cleaning the fluid path in the hematology analyzer by
“Adjusting the Flow Cell Unit pressing the Clean key on the front panel or CLEAN FLOWCELL key
Position” and “Counting the Polymer on the OPERATIONS screen and check that the CV values are
Microsphere Suspensions” sections. optimum. Adjust the gain only when the CV is optimum.
If the CV of the polymer microsphere suspensions is still not optimum

after cleaning and adjusting the flow cell position, contact your Nihon
Kohden distributor.
Sample type key 2. Press the sample type key to select PARTICLE to display the scattergram and
the peak for the polymer microsphere suspensions.
3. Press the CALC key. The appropriate setting value of FS calculated from the
NOW peak and AIM peak appears in the VALUE box.
4. Press the ENTER key. The value displayed in VALUE is entered in FS and the
cursor moves to FL.
5. Press the CALC key. The appropriate setting value of FL appears in the
VALUE box.
FS, FL
CALCULATE key
MEASURE key 6. Press the ENTER key to register the value for FL.
ENTER key
NOTE
.ccoom
In PARTICLE mode, SD gain cannot be adjusted. SD gain is adjusted
m
in fine mode by using human blood samples.
aaiill.
g mm
7. Press the MEAS key to return to the PARTICLE TEST screen to count the
g
eed@@
polymer microsphere suspensions to check the CV and peak value with the
d
adjusted gain.
n
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g m
m
hhuuo
o 8. Repeat the procedure in the “Counting Polymer Microsphere Suspensions” to
eennpp this section (“Adjusting the Gain (FS and FL) for WBC 5 Part Differential
g u
uyy
Parameters in Rough Mode Using Polymer Microsphere Suspensions”) until
you obtain the optimum peak value.
nng
Measuring Background 1. Display the READY screen and press the Count switch to count the diluent.
Noise (There is no need to set the sample.)
The result appears after measurement is complete.
TOC
Check the value.

2. Check the measured values on the result screen. Make sure that the values are
less than or equal to the following values.
WBC: 0.2 (× 103/µL)

RBC: 0.05 (× 106/µL)
HGB: 0.1 (g/dL)
PLT: 10 (× 103/µL)
TOC: 100 (count)
(TOC: total optical count. This parameter only appears in a background
noise measurement.)
Disregard the other parameter values because noise does not affect the other
parameters.
If the values are greater than the above values, check the following items, press
.ccoom
the Clean key to clean the fluid path and recount the diluent.
m
• The diluent is clean.
aaiill.
• No bubbles in the diluent.
g mm
• The apertures and aperture tubes are clean.
g
eed@@
• The aperture is firmly attached.
d
• The measurement baths and sample cup are clean.
n
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g m
m
hhuuo
o
eennpp
g u
uyy
Adjusting Gain for WBC 5 Adjust the gain for WBC 5 part differential parameters in fine mode using for
nng
Part Differential
Parameters in Fine Mode
several to 15 venous blood samples from different healthy people which are 30
minutes to 8 hours after collection.
Using Venous Blood
Samples
WARNING
Always wear rubber gloves to protect yourself from infection when
handling and measuring blood samples.
Notes about Blood Samples

• The anticoagulant may cause the blood samples to be unstable for
measurement when measured within 30 minutes after collection. For
gain adjustment, leave the samples for more than 30 minutes before
measurement.
• When a sample is stored in a cool place, such as a refrigerator, or
when the sample is left for more than 12 hours after collection, it may
affect WBC differential measurement.
• Thoroughly mix the samples immediately before measurement.
• If the blood sample is stirred too much so that bubbles are created,
the sample may be hemolyzed.
• Do not measure coagulated samples. Counting a coagulated sample
may damage the hematology analyzer.

1. Count from several to about 15 human blood samples from different healthy
people which are 30 minutes to 8 hours after collection.
2. Display the AUTO DIFF GAIN screen.

MENU → SETTINGS → SENS & THRESHOLD → AUTO DIFF GAIN
The number of data that

The scattergram serial can be registered
number The sample ID
The date and time of
measurement
The number of
The * mark appears when the
registered samples
sample is registered on the
peak list.
NOW: MO peaks of the
displayed scattergram
AVE: The average peaks of
Actual gain → Optimum the registered MO distribution
gain calculated by MO
.ccoomm AIM: The target MO peaks

peak
aaiill.
ggmm Change gain to
Return to the
ed
e
SENS&THRESHOLD
d@@ the optimum gain
Go to the PEAK LIST
screen
n
n g
g m
m Change samples to be
screen
hhuuo
o
displayed Register the displayed
sample on the peak list
eennpp
g u
uyy
nng 3. Check if the sample is appropriate to use in gain adjustment. Only register
appropriate samples for gain adjustment.
Check Points of the optimum sample for adjustment

• The distributions of NE, LY and MO are oval in shape on the scattergram.
• The distributions of NE, LY and MO are separate from each other.
• NE, LY and MO should be located in the allotted positions.
Optimum scattergram for gain adjustment
MO
NE
LY

Scattergrams which cannot be used for automatic gain adjustment (FS GAIN
too high)
(a) (b) (c)
(a) There is no NE distribution, and MO and LY are not separated on the

scattergram. (Scattergram of a leukemia patient)
(b) MO is distributed on NE. (Scattergram of a patient with immature
granulocytes)
(c) NE is distributed too close to MO. (Scattergram of a blood sample
The number of
.cc omm
collected more than 8 hours ago)
o
Registered mark registered samples
aaiill.
4. Press the ADD key to register the blood sample to the peak list. “*” is
ggmm
displayed in the scattergram serial No. after registration, and the number of N
ed
ed@@ in the MO PEAK table is incremented. Up to 12 samples can be registered.
You should have at least 5 samples registered. Press the → and ← keys to
n
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g m
m change displayed samples.
hhuuo
o
eennpp NOTE
g u
uyy
The mean value (AVE) of the registered MO distribution peak data is
updated after the sample is registered on the peak list. The gain
nng adjustment is performed using this mean value.
You can check if the obtained result is optimum on the PEAK LIST screen
before adjusting gain. For details on the PEAK LIST, refer to the operator’s
manual.
5. Press the AUTO key after finishing registration. The “ADJUST DIFF GAINS?”
message appears.
6. Press the “YES” key to change diff gains. The peak list of the registered blood
samples is initialized.

Checking the Gain 1. Count from several to about 15 human blood samples from different healthy
Adjustment people which are 30 minutes to 8 hours after collection.
2. Display the AUTO DIFF GAIN screen.

MENU → SETTINGS → SENS & THRESHOLD → AUTO DIFF GAIN
3. Register the measured samples.
4. Check that the scattergram and MO are optimum.
5. Check the scattergram in each measured data. Press the scattergram

display area to change the scattergram between MAIN, NE/EO and MO/BA.
Scattergram check points

a) The scattergrams are oval in shape and not touching the division line.
.ccoom
b) The scattergrams are in the MO, LY and NE area on the left scattergram.
m
c) There are not many scattergrams in the upper right and central area.
aaiill. c)
b)
MO
ggmm NE
ed
ed@@
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g m
m
hhuuo
o
eennpp
g u
uyy
LY
nng MO PEAK check point

AVERAGE PEAK and IDEAL PEAK are the same.
Check the values
FS AVE = 186 to 192

FL AVE = 47 to 51
SD AVE = 21 to 23
6. If the measured data is not optimum, perform the procedure in the “Adjusting
Gain for WBC 5 Part Differential Parameters in Fine Mode Using Venous
Blood Samples” section again.
NOTE
The measured data is not optimum if the human blood sample used in
the above measurement is abnormal or the gain was adjusted greatly in
the adjustment in fine mode.

Calibration
Procedure Flowchart 1. Perform calibration measurement with MEK-3DN calibrator to obtain a mean
value for calculating the new calibration coefficient.
2. Perform the calibration for CBC parameters (WBC, RBC, HGB, MCV, PLT,
RDW, MPV) using the MEK-3DN calibrator.
3. Check that the calibration coefficients for WBC 5 part differential parameters
(LY%, MO%, EO%, BA%) are 1,000 on the CALIBRATION screen. In the gain
adjustment performed in the previous section, WBC 5 part differential
parameters are already accurately calibrated. Therefore, you only need to
check the calibration coefficient.
.ccoomm
aaiill.
ggmm
Calibration for CBC
Parameters with Calibrator
eed@
Calibration can be performed for the following parameters in venous blood mode
d @
and capillary blood mode individually.
n
n g
g m
m
hhuuo
o • WBC
eennpp
• MCV
• RDW
g u
uyy • RBC
nng • PLT
• MPV
• HGB
The MEK-3DN hematology control can be used as a calibrator if it is stored at 2 to

8o C and is used within 3 days after opening. For higher accuracy, use the
calibrator soon after opening.
HCT is calibrated from RBC and MCV. Therefore, it is calibrated by calibrating

RBC and MCV.
NOTE
• Calibration with calibrator can be performed for WBC, RBC, HGB, HCT,
PLT and MPV, but not for WBC differential parameters. To calibrate
WBC differential parameters, refer to the “Calibration for WBC 5 Part
Differential Parameters” section.
• The MEK-5DN hematology control cannot be used as a calibrator.
MEK-5DN is for accuracy control.
• MEK-3DN is for accuracy control of the hematology analyzer refined
from normal human blood. The calibrator contains living cells.
Handle and store the calibrator properly following the instructions in
the assay sheet.

Measuring the MEK-3DN Hematology Control

To perform accurate calibration, you need to obtain a mean value of the calibration
measurement by measuring the MEK-3DN more than 3 times. The mean value of
the measured data can be automatically calculated and entered on the
CALIBRATION screen.
NOTE
• Check that the measurement mode is correct (venous blood mode or
capillary blood mode).
• Calibration in venous mode must be checked before calibration in
capillary mode.
• Perform the measurement for capillary blood calibration in the
capillary blood mode by pressing the Capillary mode key on the front
panel. Prepare the capillary blood samples of the volume you have
selected on the CAPILLARY screen on the MEASURE MODE screen.
.ccoom
For details, refer to the operator’s manual.
m
aaiill.
1. Display the CALIBRATION MEASURE screen.
g mm
MENU → CALIBRATION → CAL MEASURE
g
ed
ed@@
If there is no calibrator measured data stored in the memory, the “MEASURE
n
n g
g m
m CALIBRATION SAMPLE” message appears.
hhuuo
o
eennpp
g u
uyy
nng
2. Measure the calibrator. The “MEASURING” message appears on the screen.
After measurement, the measured data appears on the screen.
3. Measure the calibrator more than 3 times. The screen lists the measured data
for each measurement and the mean value of all measurements.
Up to 5 measurement data can be listed. If you measure more than 5 times, the
oldest data is deleted to store the latest data.
Mean value
Measured data
Press OK to return to
CALIBRATION screen

4. Check that there is no considerable difference in the listed data and press the
OK key to return to the CALIBRATION screen. If one data is extremely
different from the others, delete the data and measure the calibrator again to
obtain the data close to other measured data.
To delete data:
a) Press the DELETE key. The “SELECT DATA TO DELETE” message
appears.
Select the data to delete
.ccoomm DELETE key
aaiill.
ggmm
ed
ed@@
b) Press the time key of the desired data. The “DELETE DATA?” message
n
n g
g m
m appears.
hhuuo
o
eennpp
c) Press the YES key to delete the data. The screen automatically returns to
the CALIBRATION MEASURE screen after deleting the data.
g u
uyy If you press NO, the process is canceled and the screen returns to the
nng CALIBRATION MEASURE screen.
5. Press the OK key to return to the CALIBRATION screen.
NOTE
If you return to the MENU screen or go to another screen except for the
CALIBRATION screen from the CALIBRATION MEASURE screen, all
measured data are automatically deleted. After calibration, all measured
data are automatically deleted.
You can perform calibration by entering either the calibration coefficient or the
new measured value.
Calibration by Calibration Coefficient

1. Press the VENOUS key on the CALIBRATION screen to display the
CALIBRATION (VENOUS) screen.
NOTE
Write down the current settings before changing them.

2. Calculate the new calibration coefficient based on the following formula. The
calibration coefficient is on the assay sheet provided with the hematology
control and the mean measure value which were obtained in the “Measuring
the MEK-3DN Hematology Control” procedure appears in the DATA column.
.ccoomm
aaiill.
New calibration coefficient = Previous calibration coefficient ×
Calibrator assay value
ggmm Mean measured value
ed
ed@@
n
n g
g m
3. Select the desired parameter by pressing the parameter key. As you press the
m
key, the cursor moves between the DATA and CAL fields.
hhuuo
o Measured value
eennpp
Calibration coefficient
g u
uyy Parameter key
nng Setting value
4. Move the cursor to the CAL field and enter the new calibration coefficient
obtained in step 2 using the numeric keypad. The entered value appears in the
VALUE box.
5. Press the ENTER key to register the value. When you register a new
calibration coefficient, the measured data also changes. Change all necessary
values.

Calibration by Measured Data

1. Press the VENOUS key on the CALIBRATION screen to display the
CALIBRATION (VENOUS) screen.
2. Select the desired parameter by pressing the parameter key. As you press the
key, the cursor moves between the DATA and CAL fields.
Measured value Calibration coefficient
Parameter key
Setting value
Numeric keypad
.ccoomm
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e d@@
OK key to return to the
d
CALIBRATION screen
e
NEXT key to go to Enter key to register
the entered value
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another page
hhuuo
o
eennpp 3. Move the cursor to the DATA field and enter the median of the assay value
g u
uyy listed in the assay sheet using the numeric keypad. The entered value appears
nng in the VALUE box.
4. Press the ENTER key to register the value. When you register new measured
data, the calibration coefficient also changes. Change all necessary values.
NOTE
After calibration, all measured data are automatically deleted.

Calibration for WBC 5 Part Check that the calibration coefficients of LY%, MO%, EO% and BA% for WBC 5
Differential Parameters part differential parameters are 1000. In the gain adjustment performed in the
previous sections, WBC 5 part differential parameters are already accurately
calibrated. Therefore, you only need to check the calibration coefficient.
1. Display the CALIBRATION (VENOUS) screen.

MENU → CALIBRATION → VENOUS
.ccoomm
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2. Press the NEXT key to display the WBC 5 part differential parameters.
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3. Check that the calibration coefficients of LY%, MO%, EO% and BA% are
1000.
If the calibration coefficients are not 1000, move the cursor to that CAL field
and enter “1000” using the numeric keypad. Press the ENTER key to register
1000 as the calibration coefficient.

Checking the Data
Check the following data to confirm that the gain is adjusted and calibration is
performed properly.
a) Measure the MEK-5DN/L/H hematology control to check that the obtained
data falls within the acceptable range on the assay sheet attached to the
hematology control.
b) Count more than 10 venous blood samples from different healthy people
which are 30 minutes to 8 hours after collection and make sure that there is no
flag such as “Left Shift”, “Blasts”, “Immature Granulocyte”, or “Atypical
Lymphocytes” displayed.
.ccoomm
Checking Data with MEK-
a iill.
1. Press the ORDER key on the READY screen or the measured data display
a
5D Hematology Control
ggmm
screen to display the ORDER screen.
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2. Select CONTROL as the sample and select 22 as the parameters.
Press OK key to return to

READY screen.
3. Press the OK key to return to the READY screen.
4. Measure the MEK-5D hematology control. The result appears on the screen.
5. Check that the obtained data falls within the acceptable range on the assay
sheet attached to the hematology control.

Checking Data with Human Count more than 10 venous blood samples from different healthy people which are
Blood Samples 30 minutes to 8 hours after collection and make sure that there is no flag such as
“Left Shift”, “Blasts”, “Immature Granulocyte”, “Atypical Lymphocytes” or
“Eosinophilia” displayed.
WARNING
Always wear rubber gloves to protect yourself from infection when
handling and measuring blood samples.
1. Count more than 10 human blood samples from different healthy people
which are 30 minutes to 8 hours after collection.
check this.
.ccoom
2. Check that none of the following flags is displayed. Press the flag key to
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• Immature Granulocyte
• Blasts
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• Left Shift
d
• Atypical Lymphocyte
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• Eosinophilia
hhuuo
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eennpp Flag Display Screen
g u
uyy
The Flag key appears and flashes on the screen if there is flag. Press the flag
key to display the flag display screen. There is no flag if the flag key is not
nng Flag key

displayed.
Measured data
Press the flag display Press here to display the
area to return to the magnification of the data.
previous screen.
If there is a flag, repeat the procedure in the “Adjusting Gain for WBC 5 Part
Differential Parameters in Fine Mode Using Venous Blood Samples” section.
Perform the calibration again if the data obtained in the above measurement differs
from the data obtained using other instruments or at other test facilities. Calculate
the new calibration coefficient for each parameter from the mean value of measured
data obtained by the MEK-7222J/K hematology analyzer and other means.

Interference Substances
WBC: Unlysed red cells

In some rare occasions, the RBC in the blood sample may not completely lyse and these non-lysed RBC
may be detected as WBC and cause increase in WBC count.
Multiple myeloma
The precipitation of proteins in multiple myeloma patients may increase the WBC count.
Leukemia
WBC is fragile in leukemia patients and WBC may be destroyed during measurement. These WBC
fragments may also interfere with WBC differential measurement.
Chemotherapy
Cytotoxic and immunosuppressive drugs cause low WBC count.
Cryoglobulins
c oom
Cryoglobulin may be increased in patients who are pregnant or have myeloma, cancer, leukemia,
m
macroglobulinemia, lymphoproliferative disorders, metastatic tumors, autoimmune disorders, infections,
. c
a iill.
aneurysm, thromboembolic phenomena, diabetes, etc, which cause increase in WBC, RBC or PLT counts
a
ggmm
and HGB concentration. In such cases, warm the blood sample to 37°C in a water bath for 30 minutes and
RBC: Leukemia
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measure the sample immediately.
@
g m
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An increase in WBC in leukemia patient causes increase in RBC.
n
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Agglutinated RBC
e nnp
Agglutinated RBC may decrease RBC count. This can be checked by abnormal MCH and MCHC values
p
and examination of the stained blood film.
e
g u
uyy
Cold agglutinins
nng IgM immunoglobulins which are elevated in clod agglutinin disease may decrease RBC and PLT counts
and increase MCV.
HGB: Turbidity of the blood sample
Any physiologic and/or therapeutic factors may increase HGB. In such a case, determine the cause of
turbidity and follow the appropriate method below.
1. Increased WBC
An extreme increase in WBC causes excessive light scatter. In these cases, measure manually.
Centrifuge the diluted sample and measure the supernatant fluid with a spectrophotometer.
2. Increased lipids
The blood sample may be milky when there is excessive lipids. This may occur with hyperlipidemia,
hyperproteinemia and hyperbilirubinemia. Accurate HGB measurement can be achieved by manual
methods and a plasma blank.
3. Increased turbidity
When RBC are resistant to lysing, turbidity may increase causing increase in HGB. Observe if MCH
and MCHC values are abnormal. HGB result affects MCH and MCHC result.
4. Fetal bloods
The mixing of fetal and maternal blood may increase HGB value.
5. High WBC levels
Turbidity of blood increases and the hemoglobin concentration becomes high if WBC level of the
blood sample is high. MCH and MCHC levels also become high.
HCT: Agglutinated RBC
RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are
abnormal. In such a case, measure manually.

MCV: Agglutinated RBC

RBC agglutination may cause erroneous HCT and MCV values. Observe if MCH and MCHC values are
abnormal. In such a case, measure manually.
Excessive number of large PLT
Excessive number of large PLT and/or excessively high WBC may affect the MCV value. Check by careful
examination of the stained blood film.
MCH: MCH is determined from HGB and RBC values. Therefore, the limitations for HGB and RBC also affect
MCH value.
MCHC: MCHC is determined from HGB and HCT values. Therefore, the limitations for HGB and HCT also affect
MCHC value.
RDW: RDW is determined from RBC value. Therefore, the limitations for RBC also affect RDW value.
Agglutinated RBC
Agglutinated RBC may decrease RBC count and erroneous RDW. This can be checked by abnormal MCH
and MCHC values and examination of the stained blood film.
Nutritional deficiency or blood transfusion
PLT: Very small fragments

.ccoom
Iron and/or cobalamin and/or folate deficiency may increase RDW.
m
aaiill.
Very small RBC, RBC fragments and WBC fragments may be the cause in increased PLT count.
Agglutinated RBC
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eed@@
PLT may be trapped in the agglutinated RBC resulting in decrease in PLT. This can be checked by
d
Very large PLT
n
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g m
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u o
o
Large PLT may exceed the PLT threshold and might not be counted which results in low PLT count.
hh u
eennp
Chemotherapy
p
g u
uy
Cytotoxic and immunosuppressive drugs may increase the fragility of cells which may cause low PLT
y
count. In such a case, measure manually.
nng
Hemolysis
Hemolyzed specimens contain red cell stroma which may increase PLT count.
Anticoagulated blood
Blood anticoagulated with acid-citrate-dextrose may have clumped PLT which may cause decrease in PLT
count.
Agglutinated PLT
Clumped PLT may decrease PLT count and/or increase WBC count. For such sample, collect the sample in
sodium citrate anticoagulant and measure only PLT. The PLT result must be corrected for the sodium citrate
dilution effect.
MPV: Very large PLT
Large PLT may exceed the PLT threshold and not be counted which results in low MPV.
Very small fragments
Very small RBC, RBC fragments and WBC fragments may interfere with MPV measurement.
Agglutinated RBC
PLT may be trapped in the agglutinated RBC resulting in erroneous MPV. This can be checked by
Chemotherapy
Cytotoxic and immunosuppressive drugs may affect MPV. In such a case, measure manually.
NOTE
Blood samples collected in EDTA do not maintain stable MPV because platelets swell
depending on the interval after collection and storage temperature.

WBC 5 part differential parameters are derived from the WBC count, therefore, the limitations for WBC also affect these
parameters.
NE and NE%: Excessive eosinophils, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere
with an accurate NE count and NE%.
LY and LY%: Erythroblasts, certain parasites and RBC that are resistant to lysis may interfere with an accurate LY count.
MO and MO%: Large lymphocytes, atypical lymphocytes, blasts and excessive number of basophils may interfere with an
accurate MO count.
EO and EO%: Abnormal granules may interfere with an accurate EO count.
BA and BA%: Immature cell, metamyelocytes, myelocytes, promyelocytes, blasts and plasma cells may interfere with an
accurate BA count and BA%.
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Storing and Transporting the Hematology Analyzer
WARNING
This procedure must be performed to safely store and transport the
hematology analyzer. There are many expensive units installed in the
hematology analyzer. If there is any malfunction caused from not doing
this procedure, the expense necessary for performing maintenance for
that damage will be charged to you.
When storing and transporting the hematology analyzer is necessary after

demonstration, clean it by the following procedures. Clean the inside of the
.ccoom
hematology analyzer only with distilled water. After cleaning, make sure that all
m
distilled water is completely drained from the hematology analyzer.
aaiill.
g mm
If diluent is left inside the hematology analyzer, the inside of the hematology
g
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analyzer will become dirty because of dried diluent crystal or other dust. This
@
increases background noise.
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1. Press the Clean key ( ) on the front panel to clean the fluid path.
eennpp
g u
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Clean key
2. Remove the diluent tube from the ISO3 inlet, the hemolysing reagent tubes
from the HEMO3 (or HEMO3N) and HEMO5 inlets, and the detergent tubes
from the CLN3 and CLN inlets on the right side panel.
Do not disconnect the waste fluid tube from the WASTE outlet.
CAUTION
Make sure that the waste fluid tube is correctly connected.
NOTE
• To handle the diluent container, detergent container and waste
container, follow the instructions on each package.
• To handle the Hemolynac•3 (or Hemolynac•3N) and Hemolynac•5
hemolysing reagents, follow the instructions in the operator’s manual
for each hemolysing reagent.
3. Press the DRAIN ALL key on the OPERATIONS screen, and press YES key.
The hematology analyzer starts draining all fluid.

4. Connect the spare tubes to the ISO3, HEMO3 (or HEMO3N), HEMO5, CLN
YZ-0252 Cleaning bottle kit
and CLN3 inlets and put the other ends of the tubes into the distilled water.
(The optional YZ-0252 Cleaning bottle kit is available for easy setup.)
5. Press the Clean key on the front panel to prime the hematology analyzer with
distilled water.
6. Repeat steps 2 and 3 to drain all fluid from the hematology analyzer.
7. Press the main power switch on the rear panel to turn the main power off.
Main power switch
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MEK-7222J/K Hematology Analyzer Installation Check Sheet
Use this check sheet to check that the hematology analyzer is properly installed. The values in the gain adjustment and
calibration should always be entered as they are useful in troubleshooting.
Facility/Hospital Name: Serial Number:
Delivery Date: Person in charge of installation:
Checking Items OK Not OK

Preparing the hematology analyzer
1 Connect tubes
2 Connect power cord and grounding lead
3 Turn laser switch on
4 Turn power on
Date and Time Check and Cleaning
1 Check date and time settings
.ccoomm
2 Clean fluid path in the hematology analyzer
Adjusting gain and measuring background noise
aaiill.
ggmm Item Value Standard range
ed
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1
n
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Count polymer microsphere suspensions
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FS peak
FL CV%
61±3
<7.0%
hhuuo
o FL peak 90±3
2
n pp
Adjust flow cell unit position (CV% of FS and FL are <7.0%)
ee n
3
g u
uyy
Adjust FS and FL gain in rough mode
FS peak 61±3
nng FL peak
WBC
RBC
90±3
<0.2×103/µL
<0.05×106/µL
4 Measure background noise HGB <0.1 g/dL
PLT <10×103/µL
TOC <100
5 Adjust FS, FL and SD gain in fine mode
MO peak AVE AIM
FS 186 to 192
6 Check the gain adjustment
FL 47 to 51
SD 21 to 23
Calibration
1 Measure MEK-3DN hematology control
Calibration
Parameter
coefficient
WBC
2 Calibrate CBC parameters RBC
HGB
HCT
PLT
3 Calibrate WBC 5 part differential parameters
Checking data
1 Check with MEK-5D hematology control
2 Check with human blood samples
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MEK- 7222J SERVICE MANUAL

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Section 1 General .................................................................................. 1C.1

eennpp Display ............................................................................................................. 1.6

nng Power Requirements ........................................................................................ 1.6

Section 2 Troubleshooting ................................................................... 2C.1

Section3 Board/Unit Description ........................................................ 3C.1

Service Manual MEK-7222 C.1

Diluter Unit ........................................................................................................................... 3.2

nng Upper Part of Right Side ............................................................................................. 4.4

C.2 Service Manual MEK-7222

Section 5 Adjustment ............................................................................ 5C.1

eennpp Appearance ................................................................................................................ 6.1

Service Manual MEK-7222 C.3

C.4 Service Manual MEK-7222

Calibration .......................................................................................................................... 8.21

Service Manual MEK-7222 C.5

C.6 Service Manual MEK-7222

Service Manual MEK-7222 i

ii Service Manual MEK-7222

Warnings, Cautions and Notes

Service Manual MEK-7222 iii

AC power off ISOTONAC•3

.ccoomm (hemolysing reagent)

ggmm (hemolysing reagent)

nng Count Equipotential terminal

Dispense Printer socket

Auto print 1 Serial port 1

Bar code reader socket

Inlet Year of manufacture

Outlet Serial number

iv Service Manual MEK-7222

Symbol Description Symbol Description

Service Manual MEK-7222 v

eennpp Dilution Ratio ................................................................................................... 1.6

Service Manual MEK-7222 1C.1

This service manual provides useful information to qualified service personnel to

nng the fluid paths or replacing a consumable with a new one.

Service Manual MEK-7222 1.1

eennppromptly attend to your needs.

nng your instrument.

1.2 Service Manual MEK-7222

Measured Parameters, Ranges and Reproducibility to Specimen from Venous Blood

aaiill. EO%: 2 to 10%)

Standardization Analysis Method

1.4 Service Manual MEK-7222

MCV: Agglutinated RBC

PLT: Very small fragments

Service Manual MEK-7222 1.5

1.6 Service Manual MEK-7222

Dimensions and Weight

CISPR11 (1997), Group 1, Class B

Requirements for marking of IN VITRO DIAGNOSTIC instruments: EN1658 (1996)

Service Manual MEK-7222 1.7

1.8 Service Manual MEK-7222

Right Side Panel

Service Manual MEK-7222 1.9