Fruit Juices+
Fruit Juices+
Fruit Juices+
INTRODUCTION
6
EXECUTIVE SUMMARY
Soft Drinks were common preference among all the individuals before juices were being
introduced,
With the changing lifestyle and income levels, people are shifting their consumption patterns and
have therefore become more health conscious thus leading to increase in demand of juices.
The basic subject matter of the research, comparative analysis of Soft Drinks and Juices is focused
to study the mind/taste of different age group of people.
The study starts with determining the major players in the soft drinks and the juices market, their
overall consumption pattern among the people and ends up with the conclusion as per the state of
mind of the average rational human being.
7
BEVERAGE
What is beverage?
A drink, or beverage, is a liquid specifically prepared for human consumption. In addition to basic
needs, beverages form part of the culture of human society.
or
any liquid suitable for drinking; "may I take your beverage order?"
or
A liquid to consume, usually excluding water; a drink. This may include tea, coffee, liquor, beer,
milk, or soft drinks
Types of beverage
The various types of beverage are:
Alcoholic beverages
Non-Alcohol beverages
Soft drinks
Fruit juice
Hot beverages
Other
1. Alcoholic beverages
An alcoholic beverage is a drink containing ethanol, commonly known as alcohol, although
in chemistry the definition of an alcohol includes many other compounds. Alcoholic beverages,
such as wine, beer, and liquor have been part of human culture and development for 8,000 years.
2. Non-alcohol beverages
Non-alcoholic beverages are drinks that would normally contain alcohol, such as beer and wine but
are made with less than .5 percent alcohol by volume. The category includes drinks that have
undergone an alcohol removal process such as non-alcoholic beers and de-alcohol zed wines.
8
Non-alcoholic variants:
Low alcohol beer
Non-alcoholic wine
Sparkling cider
3. Soft drinks
The name "soft drink" specifies a lack of alcohol by way of contrast to the term "hard drink" and
the term "drink", the latter of which is nominally neutral but often carries connotations of alcoholic
content. Beverages like colas, sparkling water, iced tea, lemonade, squash, and fruit are among the
most common types of soft drinks, while hot chocolate, hot tea, coffee, milk, tap water, alcohol,
and milkshakes do not fall into this classification. Many carbonated soft drinks are optionally
available in versions sweetened with sugars or with non-caloric sweeteners.
4. Fruit juice
Juice is a liquid naturally contained in fruit or vegetable tissue. Juice is prepared by mechanically
squeezing or macerating fresh fruits or vegetables without the application of heat or solvents. For
example,orange juice is the liquid extract of the fruit of the orange tree. Juice may be prepared in
the home from fresh fruits and vegetables using variety of hand or electric juicers. Many
commercial juices are filtered to remove fiber or pulp, but high pulp fresh orange juice is a popular
beverage. Juice may be marketed inconcentrate form, sometimes frozen, requiring the user to
add water to reconstitute the liquid back to its "original state"
9
5.Hot beverages
Hot beverages, including infusions. Sometimes drunk chilled.
Coffee-based beverages
Cappuccino
Coffee
Espresso
Café au lait
Frappé
Flavored coffees (mocha etc.)
Latte
Hot chocolate
Hot cider
Mulled cider
Tea-based beverages
Flavored teas (chai etc.)
Green tea
Pearl milk tea
Tea
Herbal teas
Yerba Mate
Roasted grain beverages
Sanka
6.Other
Some substances may either be called food or drink, and accordingly be eaten with a spoon or
drunk, depending on solid ingredients in it and on how thick it is, and on preference:
Soup
Yogurt
OVERVIEW OF THE INDUSTRY
10
The beverage market is worth $55 billion worldwide.
The tides are turning for many beverage categories. While the carbonated soft drink and beer
categories are merely treading water with flat sales, the energy drink category is surging ahead like
never before. Bottled water, ready-to-drink coffee, ready-to-drink tea and sports drinks follow close
behind with substantial sales increase- drinks without added sugar, no beer, along with
developments in juice drinks and dairy-based drinks, are helping to turn around sales in these
categories. What follows is a category-by-category look at the state of the beverage industry,
including the top brands, new products, innovations and future trendsetters.
In order to be successful in the marketplace, one has to think in terms of health innovation, flavor
innovation, ingredient innovation and specific age groups. These are the factors that will shape the
future of the beverage industry.
“Today’s consumers are concerned with overall health and wellness. As a result, there is significant
impact on food and beverage purchases. Many studies have shown that consumers are as concerned
with good health as they are about maintaining a high quality of life.”
Do you know what type of new beverage consumers are most likely to try? Do you know where
they are most likely to pick those products up? Do you know why?
Beverage Industry wanted to know the answers to these questions and to delve deeper into the
ever-increasing number of new product launches in the beverage market. “The soft drink industry
is training people to seek out new products, even the big guys are coming out with limited-edition
flavors, and consumers are beginning to see that there is more flavor activity going on in the
category. Whether that really nets anybody any sales gains is another thing, but it is teaching
consumers to seek out and try new products. It’s also trying to create some excitement there.”
11
In spite of several challenges and restrictions faced by this industry, it is a ‘roll’ like never before.
Customer preferences may have shifted, but they are still always on the look out for a can of ‘coke’
or a new ‘flavored’ drink to quench their thirst.
12
13
RECENT ISSUES
One of the aspirants is Xtazy, an energy drink from the US. “Xtazy is the forth largest energy
drink inthe US”, says Rohan Malhotra, Managing Director of R. M. Indian Liquor Pvt. Ltd., the
exclusive importer for Xtazy. Malhotra has launched Xtazy already in Eastern India, and was
looking now for a distributor in the Delhi area, when FII spoke to him during IFE fair in Delhi
recently.
In order to take on Red Bull and get a share of 20% from their business, Malhotra wants to
offer better conditions to distributors. “We provide a margin of 6-8% to a distributor, who thus can
earn about Rs 6 a can”, Malhotra says. “ This is more than what Red Bull offers which is only
about 2 – 3 Rs per can.” Xtazy is available in cans of 350 ml, thus more than the usual 250 ml of
other energy drinks, and will be priced with a MRP of Rs 85. Four variants are offered, Cranberry
Blast, Sugar Free Passion Fruit-Pineapple, Orange Blast and Lime Blast.
Malhotra has planned several marketing measures to promote Xtazy, like PoS actions, coupons
and direct marketing in a first round, and night parties in a second round. In marketing
communication, he is highlighting not only the variants, but also health effects as the USPs of
Xtazy, which are derived from ancient herbs used in the drink like gingko and guarana. The extract
of gingko biloba leaf has been shown to dilate blood vessels and has the ability to increase
peripheral blood circulation, especially to the brain, the company writes in a leaflet. Guarana from
Brasil would serve to promote weight loss by increasing the metabolic rate and reducing the
appetite.
Besides the US, Malhotra says, Xtazy would be marketed also in Israel, Ukraine and in Turkey
and would soon be launched in China.
14
2. Australia-based "Aromas" launches first coffee store in India.
Australia-based "Aromas," one of the leading coffee chain shops, launched its first café outlet in
India at Hiranandani, Powai, and Mumbai. The company also tied up with Ideal Hospitality Private
Ltd (IHPL), which would own the brand in the country and south-east Asia.
Jayant Mahiskar, chairman and MD, IHPL, said, "Aromas is being launched keeping in mind the
true coffee connoisseurs. We aim at targeting the youth and corporate at our outlets and Powai with
a mix of residential and office complexes was a natural choice. The coffee has been created from
selection of the original beans and blended to suit all tastes. Aromas ensures freshness and fullness
of flavor." According to the agreement with Aromas, IHPL will pay about 1.5% of the gross
revenue earned.
Further, the company has decided to invest about Rs. 50-75 crore in the next three years to expand
its footprint with 99 outlets in India.
Coca-Cola's bid in September prompted an outcry by nationalists who urged the government to bar
foreigners from acquiring one of China's most successful homegrown brands. Rival juice producers
warned that the acquisition would give Coca-Cola too dominant a position in China's beverage
market. A Coca-Cola spokesman in Hong Kong learned of the rejection of the sale had no
immediate comment. Huiyuan's founders and major shareholders already had endorsed the sale.
If Coke were to take over Huiyuan, it will dominate the soft drinks market in China, which not only
hurts consumers, but also other sector participants. Huiyuan controls more than a tenth of the
Chinese fruit and vegetable juice market that grew 15% last year to $2 billion. Coca-Cola has a
9.7% share and dominates in diluted juices.
15
According to analysts China's ruling on Coke could cut both ways in that Chinese firms that have
been making increasingly high profile acquisitions abroad may run into trouble of their own.
Pepsi has now opted for a new brand ambassador, Katrina Kaif.
"The creative thought behind the new communication was to further enhance the Slice experience
into dimensions of pleasure, sensuality and indulgence. Last year's commercial was about
enumerating the principles of 'Aamsutra' or the art of experiencing pure mango pleasure with the
new Slice. This year, the commercial portrays the next level to bring alive the mango indulgence,
stated Hari Krishnan, Vice President, JWT.
The company has now opted for a 360 multimedia campaigns involving digital, print, radio, impact
outdoors and sampling in core markets.
On the occasion of LMN's launch, Nadia Chauhan, joint managing director and CMO, Parle Agro,
said, "Nimbu pani has traditionally been India's most commonly consumed cold beverage. In fact
the idea of a branded lemon drink is so simple that you would wonder why nobody thought of it
earlier. The challenge for us was packaging a natural product while retaining its fresh, original taste
throughout its shelf life."
LMN will be available in 110 ml Tetra, 200 ml Tetra and 500 ml PET packs priced at Rs 5, Rs 10
and Rs 23 respectively. The company aims to touch a turnover of Rs 3000-3500 crore by 2011.
The company will target both (youth and adult) segments of consumers to turn them into branded
consumers of nimbu pani. Besides this LMN will also target an emerging segment of consumers
who are looking for a healthy and refreshing beverage in the country. "For the last 20 years, Parle
Agro has been the market leader in fruit based beverages, we have constantly worked keeping in
mind Indian preferences while formulating products that cater to the Indian palate. It is without any
doubt that only an Indian company can understand what real nimbu pani tastes like and what the
Indian consumer wants in a packaged offering," Chauhan added.
Further, the company claims that packaged nimbu pani will have tremendous growth potential,
higher than other packaged drinks mainly because of a major shift in consumer behavior. Today,
the beverage consumer is looking for hygiene, convenience, refreshing taste, affordability and year-
round availability. The name LMN is derived from the SMS version of the word lemon. Parle Agro
also owns other fruit drink brands like Frooti, Appy Fizz and packaged drinking water, Bailey.
6. PepsiCo launches 'Nimbooz,' packaged lemon juice with no fizz and artificial flavours
PepsiCo India has launched its packaged nimbu paani, Nimbooz, under its 7Up brand. The home-
made nimbu paani or lime juice has been specially created to suit Indian tastes.
The lemon juice, no fizz and artificial flavours, is available in trendy, convenient packs.
The drink offers great value to consumers in three packaging formats of 200 ml returnable glass
bottles (RGB), 350 ml PET and 200 ml Tetra attractively priced at Rs 10, Rs 15 and Rs 10,
17
respectively.
According to Ms Punita Lal, Executive Director- Marketing, PepsiCo India, Nimbooz, is specially
developed to suit Indian tastes and preferences.
"Nimbooz is an affordable offering for consumers on the go because of its ready-to-drink format
that is both convenient and hygienic. The proposition of the Indian refresher perfectly captures the
mass appeal of this product and will certainly drive consumer connect, stated Ms Alpana Titus,
Executive VP-Flavours, PepsiCo India.
PepsiCo has drawn up an intensive consumer activation campaign to market Nimbooz. The 360
degree marketing communication plan will revolve around building awareness through multi-city
launches and road shows, comprehensive 3D activation, leveraging Out-of-Home (OOH) media,
radio, press and outdoors. Aggressive trial generation and sampling initiatives will also be taken
forward across major cities of the country. A special 'Nimbooz Highway Gadi' has been created
that will visit the four major highways connecting Delhi to Jaipur, Dehradun, Agra to drive trails
and consumer education.
During the Fanta Apple launch in October 2008, Venkatesh Kini, marketing vice-president, Coca-
Cola India, said that the company had planned to reach about 3.5 lakh customers with sample apple
flavoured drink to extend its market leadership in the fruit flavoured segment in Andhra Pradesh
and Tamil Nadu.
"As per consumer research, we have found that after orange, apple is the most preferred fruit in the
country and Fanta Apple has been developed specially for the Indian palate," Kini said on Monday.
According to experts, the nationwide launch of Fanta Apple is a part of the company's $250 million
business plan for the country.
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Fanta Apple is the second flavour after Fanta Orange under "Fanta" brand of the company. "We
have had an excellent response down south with a reused value to the drink and with the national
launch of Fanta Apple, we are stepping stones to extend Coca Cola India's market leadership in the
fruit-flavoured sparkling drink segment," Kini added. The company has also announced Bollywood
actress Genelia D'Souza as the new brand ambassador of the Fanta brand.
According to reports, the current expected Indian soft drink market is about Rs 6,000 crore, in
which the company shares about 50% market with its various brands like Coke, 7 Up, Fanta, Sprite
and Thums Up.
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STUDY OF GROWTH OF SOFT DRINK MARKET
SOFT DRINKS
Carbonated drinks are dominated by artificial flavors based on cola, orange and lime with Pepsi
and coca-cola dominating the market. The entire part of the drink is based on its artificial flavors
and sweetening agents as no natural juice is used.
MARKET
Cola products account for nearly 61-62% of the total soft drinks market.
Two global majors’ Pepsi and coke dominate the soft drink market.
NCAER survey says 91% of soft drink in the country is in the lower, lower middle and
upper middle class people.
The market is worth around Rs.5000 crores with growth rate of around 10-15%.
The annual per capita consumption in India is only about 6 bottles vis- a- Vis 340 bottles in
the U.S.
The production as soft drinks has increased from 5670 million bottles in 1998-99 to 6230
million bottles in 1999-2000 industry source.
Growth market this year is expected to be 10-15% in value terms and 20-22% in volume
terms.
However, the market for carbonated drinks is stagnating and not growing as expected.
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MAJOR PLAYERS IN SOFT DRINKS SEGMENT
Coca cola has truly remarkable heritage. From a humble beginning in 1886 it has now become
the flagship brand of largest manufacturer, distributor of non alcoholic beverages in the world.
In India, coca cola was the leading soft drink till 1977 when govt. policies necessitated its
departure. Coca cola has made its return to the country in 1993.and made significant investment to
ensure that the beverage is available to more and more people in remote as well as inaccessible
parts of the world.
Coca cola returned to India in 1993 and over the past ten years has captured the imagination of the
nation, building strong association with cricket, the thriving cinema industry, music etc. coca cola
has been very strongly associated with cricket, sponsoring the world cup in 1996.
In 2002, coca cola launched the campaign,”Thanda Matlab coca cola”. in 2003,coke was available
for just rs,5 crores in the country.
21
FANTA : GHOONTH BHAR SHARARAT KAR LEY!!!
Fanta entered the Indian market in year 1996 under the coca cola brand .over the years, Fanta
has occupied a strong market place and is identified as “the fun catalyst”. Fanta stands for its
vibrant color, tempting taste and tingling bubbles that not just uplifts feelings but also helps free
spirit thus encouraging one to indulge in the moment.
Drink that can cast a tangy refreshing spell on anyone, anywhere. Born in 1971, Limca has been
the original thirst choice, of millions of consumers for over three decades.
The brand has been displaying healthy volume growing year on year and limca continues to be
leading flavoring soft drinks in the country.
Dive into the zingy refreshment of limca and walk away a new person.
22
SPIRITE: SPIRITE BHUJAYE PYAAS BAKI SAB BAKWAAS!!!
World wide sprite ranked as no.4 soft drink and is sold in more than 190 countries In India, sprite
was launched in year 1999 and today it has grown to be one of the fastest growing soft drinks,
leading clear lime category.
Today sprite is perceived as a youth icon. With strong appeal to youth sprite has stood for a straight
forward and honest attitude. Its clear crisp hingtaste encourages today’s youth to trust their
instincts, influence them to be true who they are and to obey their thirst.
Maaza was launched in 1976. In 1993, maaza was acquired by coca cola India. Maaza currently
dominates the fruit drink category. Over the years, maaza has become synonymous with mango.
“Taaza Mango, Maaza mango, Botal mei aam, maaza hai naam”.consumers regard maaza as
wholesome, natural, fun loving drink real experience of fruit.
The campaign builds on the existing equity of the brand and delivers a relevant emotional benefit
to the moms rightly captured in tagline, “yaari dosti, and taaza maaza”.
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PEPSI: YEH DIL MAANGE MORE!!!
Pepsi cola is a carbonated beverage that is produced and manufactured by Pepsi co. It is sold in
stores, restaurants and from vending machines. The drink was first made in the 1890’s in North
Carolina.
The brand was trademarked on June 16, 1903.There have been many Pepsi variants produced over
the years.
Diet Pepsi
Crystal Pepsi
Pepsi twist
Pepsi max
Pepsi samba
Pepsi blue
Pepsi gold
Pepsi holiday spice
Pepsi jazz
Pepsi x(available in Finland & brazil)
Pepsi next(available in Japan & south Korea)
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STUDY OF GROWTH OF FRUIT DRINK MARKET
FRUIT JUICES
Usually confused and considered synonymous with non-aerated drinks, fruit pulps, juices and
squash are high sugar beverages, which are centrifuged and filtered to give a semi- clear
appearance.
In the past, this sector enjoyed an excise exemption, keeping cost at minimal. However the
withdrawal of exemption has inflated costs and can affect growth, with dramatic change possible
on reintroduction of excise exemption.
MARKET
The organized fruit beverage market is estimated at Rs.500 crores market. (Nectars, drinks
and juices combined).
The market has grown at a 20% to 25% rate.
Of this, more expensive juices segment has grown at rate of 40%this year. It accounted for
only 15% of the fruit beverage 3 years back.
In –home consumption of juices has gone up from 30%, three years back to 80%today.
Mango based drinks account for two thirds of fruit drinks industry.
26
MAJOR PLAYERS IN THE FRUIT DRINK SEGMENT
DABUR REAL
Dabur’s flagship brand real fruit juice is a market leader in packaged fruit juice category. Real was
launched in 1996 and the brand has carved a niche for itself by claiming to be the only fruit juice in
packaged form .i.e.100%preservative free.
Real, with market share of 57% comes in nine flavors:
Orange
Mango
Pineapple
Mix fruit
Grape
Guava
Litchi
Tomato
Cranberry
Real Active is 100%fruit juice with no added sugar and is available in following variants:
Orange
Apple
Orange- carrot
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GODREJ
The food division of godrej industry produces and market fruit drinks, fruit nectar and sofit
soymilk. Godrej’s brand JUMPIN comes in the following flavor:
Mango
Pineapple
Apple
Litchi
Orange
Godrej’s x’s is a range of fruit nectar with more fruits. It’s available in following flavors:
Orange
Apple
Grape
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PEPSI’S TROPICANA
Tropicana brand fruit juice enjoys a market share of 25% and has registered a double digit growth
and has outpaced the growth of fruit juice market in India. It is available in following flavors:
Orange
Apple
Grape
Cranberry
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LEH BERRY
It is a product from Ladakh Foods. Its first fruit juice in Delhi and it's selling it in the more affluent
parts of town. It enjoys a market share of 4% and is available in a variety of flavors:
Pineapple
Apple
Mixed fruit
Orange
Blackcurrant
Mango
Guava
30
PARLE’S FROOTI and APPY
Frooti was launched back in 1985 and enjoys market dominance with 85% of market share. Parle’s
Agro’s APPY, in 1996 had a market share of 5% in the fruit drink segment; in 2003 its new
variant-APPY FIZZ was launched.
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OBJECTIVE OF THE STUDY
To study the preferences of the people for soft drinks and fruit juices.
To find out the factor(s) that influences the consumer’s consumption of soft drinks and fruit
juices.
To test the know-how of the consumers regarding the various existing brands of soft drinks
and fruit juices.
To explore the next best beverage after soft drinks and fruit juices.
To find out how the beverage is positioned in the mind of the consumers.
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SCOPE OF THE STUDY
This study is confined to the North West Delhi region covering areas of
Paschim Vihar, Pitam Pura, Rohini area, Punjabi Bagh.
The main aim of this research study is to analyze the preference of people (of different age groups)
on consumption patterns of Soft Drinks and Fruit Juices.
33
Chapter-2
Review of literature
34
REVIEW OF LITERATURE
Fruit juice
The manufacture of juices from fruits and vegetables is as old (or older) than agriculture. In
simple words, juice is the extractable fluid contents of cells or tissues. It is defined as
fermentable but unfermented juice, intended for direct consumption, obtained by the
mechanical process from sound, ripe fruits, preserved exclusively by physical means. The
juice may be turbid or clear. The addition of sugars or acids can be permitted but must be
endorsed in the individual standard (Bates et al., 2001; ICMSF, 2005; Bevilacqua et al.,
2011).
Fruits and vegetables form a versatile and complex substance group category of
foods. The relevant substance groups are carbohydrates, acids, minerals, polyphenols
(tannins) including the colourful anthocyanins, water-soluble vitamins, amino acids, aroma
compounds, carotenoids, fibers and other bioactive substances. During processing, they are
essentially transferred into the pressed juice or into the puree (Bates et al., 2001).
35
pH of fruit juices
Fruit juices usually have low pH values that range between 2.0 and 4.5. Lime or lemons have
the lowest pH. The low pH of fruit juices is due to the presence of organic acids which varies
with the different type of juices (Table 2.1).
36
Table 2.1. Typical pH values and the naturally occurring organic acids in fruit
juices*
Spoilage
Food spoilage is defined as a change in the appearance, smell or taste of a food that makes it
unacceptable to the consumer (Aneja et al., 2008). Spoilage of fruit and vegetable juices is
primarily due to the proliferation of their natural acid tolerant and osmophillic microflora.
They contain high levels of sugar and possess ideal water activity for microbial growth; their
low pH (Table 2.1) makes them more susceptible to yeast and fungal spoilage because a big
7
part of bacterial contamination is eliminated due to the preference of bacteria to grow at
neutral pH (Worbo and Splittoesser, 2004; Patil et al., 2011; Bevilacqua et al., 2012).
Sources of contamination
Fruits and vegetables commonly used in juice processing are exposed to variety of potential
spoilage microorganisms during agricultural production, harvesting and transportation to fruit
sorting and juice extraction facilities. Most microorganisms that are initially observed on
37
whole fruit surfaces are soil inhabitants. Vectors for disseminating the microbes include soil
particles, airborne spores and irrigation water. Fruit and fruit juices are contaminated with
yeasts and moulds often from insect damage. Flavourings, water, processing machinery,
filling lines and other chemicals are all potential sources of microbial contamination
(Wareing and Davenport, 2005; Barth et al., 2009; Lawlor et al., 2009).
Spoilage microorganisms
Fresh fruit juices are more susceptible to spoilage because fluid contents are in touch with air
and microbes from the environment during the time of handling. Yeasts, heat sensitive
moulds and lactic acid bacteria are indicator for the quality of raw materials. Heat resistant
fungi and other spore forming bacteria such as Clostridium pasteurianum and Bacillus
coagulans are used as targets for fruit juice pasteurization processes (Tribst et al., 2009). The
various group of organisms involved in spoilage of various fruits, fruit products and fruit
juices are described here.
Yeasts
Yeasts predominate in the spoilage of acid fruit products because of high tolerance, frequent
ability to grow anaerobically and certain species are preservative resistance. More than 110
species of yeasts have been associated with foods; of which large proportion occur on fruits.
The presence of yeasts in fruit juices may result from failures in fruit juice pasteurization and
in sanitation practices. Pichia, Candida, Hansenula, Rhodotorula, Saccharomyces,
Torulopsis, Trichosporon and Zygossacharomyces, are some well known and important food
spoiling yeasts. Yeast species that cause spoilage in citrus fruits are Candida parapsilosis, C.
stellata, Saccharomyces cerevisiae, Torulaspora delbrueckii and Zygosaccharomyces rouxii
(Arias et al., 2002; ICMSF, 2005; Stratford, 2006; Tribst et al., 2009; Bevilacqua et al.,
2011; Vantarakis et al., 2011; Patil et al., 2011; Tyagi et al., 2013, 2014; Bukvicki et al.,
2014).
8
Tournas et al. (2006) also reported the presence of Rhodotorula rubra, C. sake,
Kloeckera apis and C. lambica being the most frequently encountered organism from apple,
carrot, grape, grapefruit and orange juices. Candida spp., Trichosporon mucoides, Kloeckera
sp., yeast-like fungus Cryptococcus neoformans were observed in freshly squeezed juices of
orange, lemon, grapefruit, and apple. Spoilage by yeasts in fruit juices is characterized by
formation of CO2 and alcohol. Yeasts may also produce turbidity, flocculation, pellicles, and
38
clumping. Yeasts also produced pectinesterases which degrade pectin causing spoilage,
organic acids, and acetaldehyde, which contribute for a “fermented flavor,” may also be
formed (Lawlor et al., 2009).
Yeasts resistant to preservatives
Resistance to preservatives is a great threat to the stability of fruit juices. Examples of yeasts
resistant to preservatives include Zygosaccharomyces bailli, Candida krusei, Saccharomyces
bisporus, Schizosaccharomyces pombe and Pichia membranarfaciens. Resistance to
preservatives has been attributed to the ability of cells to tolerate chronic intracellular pH
drops by phosphofructokinase enzyme. P. membranifaciens is resistant to heat, moderate
amount of salt, SO2, sorbic, benzoic and acetic acid hence it is considered as target
microorganism for optimization of thermal pasteurization of fruit juices (ICMSF, 2005;
Lenovich et al., 2006; Stratford, 2006; Bevilacqua et al., 2011; Tyagi et al., 2013, 2014).
Moulds
Mould spoilage in fruits and fruit juices is divided into two categories:-
1) Growth of mould due to poor hygiene within factory or field conditions.
2) Growth of heat resistant moulds within heat processed juices.
The former type can cause tainting, discoloration and other problems associated with gross
mould growth. The latter type can result in slow growth of the mould within the processed
product. Juice cloud loss occurs through the activity of pectin esterases. The dominant
moulds recorded in fruit juices belong to Penicillium sp., Cladosporium sp., Aspergillus
niger, A. fumigatus, Botrytis sp., Aureobasidium pullulans. Rhizopus and Mucor are also
associated with spoilage of fresh fruits and vegetables (ICMSF, 2005; Wareing and
Davenport, 2005; Tournas et al., 2006; Moss, 2008; Lawlar et al., 2009).
Aspergillus and Peniciliium were the dominant mould genera isolated from orange,
guava and banana juices freshly prepared from the respective fruits collected from the local
9
markets of Zagazig city, Sharkia Govenmorate, Egypt (Helal et al., 2006). Penicillium,
Fusarium and Geotrichum were reported in pasteurized grapefruit juice (Tournas et al.,
2006). Among these, some moulds produce mycotoxins which are of great threat to human
health. Major mycotoxins associated with fruit juices are byssochlamic acid (Byssochlamys
fulva, B.nivea), patulin (B. fulva, B. nivea, P. expansum), ochratoxin (Aspergillus
carbonarius) and citrinin (Penicillium expansum, P. citrinum) (Delage et al., 2003; Wareing
39
and Davenport, 2005). Presence of patulin in fruit juices is indicator of poor quality of fruits
used in processing of juices (Sylos et al., 1999).
Heat resistant fungi
Spoilage of pasteurized fruit juices is caused by heat resistant fungi. Principal heat resistant
moulds belong to Byssochlamys nivea, B. fulva, Neosartorya fischeri, Eupenicillium
brefeldianum and Talaromyces macrospores. These moulds survive commercial heat
pasteurization treatment, usually applied to fruits and fruit products, due to the presence of
heat resistant ascospores. Byssochlamys spp. are historically most widely encountered
moulds causing spoilage of heat processed fruits (ICMSF, 2005; Salomao et al., 2007;
Lawlor et al., 2009). Kutama et al. (2010) reported the presence of heat resistant moulds such
as Byssochlamys, Neosartorya and Talaromyces in orange, mango, tomato and pineapple
juices. The presence of heat resistant fungi such as Paecilomyces variotii, Aspergillus tamari,
A. flavus and A. ochraceous has been reported in sixty packaged Nigerian fruit juices
consisting of mango, pineapple, orange and tomato (Obeta and Ugwuyani, 2007).
Chlamydospores, sclerotia and aleurospores are the resistant structures/spores produced by
these moulds (Voldrich et al., 2004; Salomao et al., 2007).
Bacteria
Bacteria are usually present in low numbers on fresh fruits and vegetables. Some bacteria
such as heterofermentative lactic acid bacteria (LAB), acetic acid bacteria, Erwinia sp.,
Enterobacter sp., Clostridium, Alicyclobacillus acidoterristeris, Propionibactreium
cyclohexanicum, Pseudomonas sp. and Bacillus have been reported as deteriorative in cut
fruits and juices (ICMSF, 2005; Lawlor et al., 2009; Raybaudi-Massilia et al., 2009b; Tribst
et al., 2009; Bevilacqua et al., 2011).
40
metabolites in spoiled fruit juices, contributing to buttery or butter milk off flavor to citrus
juices (ICMSF, 2005; Lawlor et al., 2009; Steyn et al., 2011).
Alicyclobacilli
al., 2011). Alicyclobacillus In recent years, Alicyclobacillus a thermoacidohile, endospore
producing bacterium has emerged as major concern to the beverage industry worldwide as
many high concentrated fruit products which are valuable semi prepared food components to
the bakery, dairy, canning, baby foods, distilling and beverage industries have been found to
be contaminated with these spoilage microbes. The thermoacidophile nature and presence of
highly resistant endospores is responsible for their survival during the production of
concentrated fruit products. Soil is considered to be the main source of contamination of
fresh fruits during harvesting (Walls and Chuyate, 2000; Parish and Goodrich, 2005; Bahceci
et al., 2007; Groenewald et al., 2008, 2009; Steyn et al., 2011).
Of the over 20 species of Alicyclobacillus isolated from different environments. A.
acidocaldarious, A. hesperidium, A. acidophilus, A. cyclohaptanicus, A. fastidious and A.
pomorum have been implicated in spoilage incidents in high acid fruit and vegetable products
(Goto et al., 2007). Alicyclobacillus acidoterrestris has emerged as new spoilage bacterium
for commercialized fruit juices that can survive pasteurization at 95 oC for 2 minutes and can
spoil heat treated fruit juices by the formation of taint chemicals (guaiacol and halophenolic)
(Witthuhn et al., 2007; Steyn et contains ω- alicyclic fatty acids (ω-cyclohexane and ω-
cycloheptane fatty acids) in their cell membrane that are responsible for heat resistance of by
1
forming a protective coating with strong hydrophobic bonds. These 1 hydrophobic bonds
41
calcium- dipicolinate complex (Wisotzsky et al., 1992; Chang and Kang, 2004; Jay et al.,
2005; Smit et al., 2017
Contamination of Alicyclobacillus in fruit juices results from sources like soil, water and
processing facilities. Spoilage of fruit juices by Alicyclobacillius is difficult to detect because it
does not produce any visible changes such as gas during growth and incipient swelling of
containers does not occur so that spoilage in retail products cannot be noticed. It produces a
smoky, medicinal and antiseptic off odour associated with guaiacol. Other compounds such as
2,6- dibromophenol and 2,6- dichlorophenol have also been detected (Silva and Gibbs, 2004;
Witthuhn et al., 2007; Durak et al., 2010; Danyluk et al., 2011; Smit et al., 2011; Witthuhn et al.,
2013). Endospores of Alicyclobacillus have D values in the range of 16-23 minutes at 900C,
greater than the pasteurization treatments applied in fruit juice processing (Walker and Phillips,
2008b). Hence, Silva and Gibbs (2004) suggested that Alicyclobacillus be designated as the target
microbe in the design of pasteurization processes for acidic foods and beverages.
Propionibacterium cyclohexanicum
Propionibacterium cyclohexanicum was first isolated from spoiled orange juice in 1993. It
possesses ω-cyclohexyl undecanoic acid in cell membrane as Alicyclobacillus genus but
lacks the production of endospores (Kusano et al., 1997; Walker and Phillips, 2008a).
Walker and Phillips (2007) reported that P. cyclohexanicum survives at 950C for 10 minutes
in orange juice and hence would survive treatments commonly used in pasteurization process
used in fruit juice industry.
Bacillus
Bacillus coagulans, B. marcesens and B. polymyxa spoil several fruit juices (Stratford et al.,
2000). B. coagulans spoils canned tomato juice and vegetable products. It causes flat sour
spoilage in juice (ICMSF, 2005; Silva and Gibbs, 2004; Steyn et al., 2011; Daryaei and
Balasubramanium, 2013).
Clostridium 1
2
Two species of Clostridium mainly C. pasteurianum and C. butyricum have been isolated at
low pH of fruit juices (Stratford et al., 2000).
42
Members of Enterobacteriaceae
Cedecea sp., are capable of multiplying in citrus juices with pH values below 4.3. These
strains Psychrotrophic bacteria such as Klebsiella sp., Serratia sp., Citrobacter sp., and cause
a mixed-acid fermentation resulting in citrate, acetate, and CO 2 production, along
with“unclean” flavor and aroma defects. In certain cases, enteric bacteria may produce a
sulfur- like off-aroma in spoiled citrus juices (Lawlor et al., 2009). Suguna et al. (2011)
observed the presence of Klebsiella pneumoniae in dragon-fruit (pitaya) juices in Penang city
of Malaysia.
43
Pathogenic microorganisms
In tropical countries consumer preference is for fresh-cut fruits and juices rather than their
processed counterparts. Fruit juices sold by the street vendors are consumed regularly by
the local owing to their fresh look, original nutritional and sensory attributes (Brackett,
2001; Thunberg et al., 2002; Suguna et al., 2011). In India, a large population of all income
and age groups consume freshly squeezed fruit and vegetable juice, but the presence of
pathogenic microorganisms in street vended fruit juices have been reported in various parts
of India such as Vishakhapatnam (Lewis et al., 2006), Mumbai (Mahale et al., 2008),
Amravati (Tambaker et al., 2009), Nagpur (Titarmare et al., 2009), Kolkata
(Mukhopadhyay et al., 2011), Mysore (Divyashree et al., 2013) and Tirumula (Suneetha et
al., 2013). Other researchers have also carried out study on the microbiological quality of
street vended fruit juices in other parts of the world as summarized in table 2.2. Food borne
pathogens such as Escherichia coli and Salmonella survive in acidic environment of fruit
juices due to acid stress response (Ghenghesh et al., 2005; Tribst et al., 2009; Ray-Baudi
Massilia et al, 2009). Some strains of
E. coli, Shigella and Salmonella may survive for several days and even weeks in acidic
environment by regulating their internal pH that maintained at neutral pH by combination
of passive and active mechanisms (Vantarakis et al., 2011).
Shigella flexneri and S. sonnei survive in apple (pH 3.3) and tomato juices (pH
4.0) at 7 C for at least 14 days (Opstal et al., 2005). Sospedra et al. (2012) reported the
0
44
Fig.2.2. Amomum subulatum- plant, fruits and Fig.2.3.Cinnamomum tamala- plant
with seeds (inset) leaves
45
Fig.2.7. Curcuma longa- rhizome Fig.2.8. Elettaria cardamomum-
plant and fruits (inset)
46
Fig. 2.12. Illicium verum- fruits Fig. 2.13. Mentha arvensis- plan
47
Chapter 3
RESEARCH METHODOLOGY
48
METHODOLOGY
RESEARCH DESIGN
EXPLORATORY RESEARCH
CONCLUSIVE RESEARCH
49
Exploratory research is one type of research design, which has its primary
objective the provision of insights into, and comprehension of, the problem situation
confronting the researcher.
Conclusive research is designed to assist the decision maker in determining
evaluating and selecting the best course of action to take in a given situation.
Descriptive study as the name implies is designed to describe something-for example the
characteristics of users of a given product, the degree to which the product use the varies
with income, age, etc.
1. Primary data
2. Secondary data
50
1) PRIMARY DATA:
Questionnaire: Primary data was collected by preparing questionnaire and the people
were randomly being requested to fill them.
2) SECONDARY DATA:
Secondary data will consist of different literatures like books which are published,
articles, internet and websites.
In order to reach relevant conclusion, research work needed to be designed in a proper
way.
The main statistical tools used for the collection and analyses of data in this project are:
Questionnaire
Pie Charts
Bar Diagrams
51
CHAPTER 4
DATA ANALYSIS & FINDINGS
52
Q1. What do you prefer to drink? (Tick any 1)
60% 57%
50%
43%
No. of Respondents
40%
30% Series1
20%
10%
0%
Soft Drinks Fruit juices
ANALYSIS:
53
Q2. Frequency of consumption of your preferred drink in a week?
SOFT DRINKS
Daily 28 49%
2-6 times 23 40%
Above 7 6 11%
Total 57 100%
60%
49%
50%
40%
No. of Respondents
40%
30% Series1
20%
11.00%
10%
0%
Daily 2-6 times Above 7
Weekly Consumption
ANALYSIS:
54
FRUIT JUICES
Daily 10 23%
2-6 times 30 70%
Above 7 3 7%
Total 43 100%
35
30
30
25
No. of respondents
20
Series1
15
10
10
5 3
0
Daily 2-6 times Above 7
weekly consumption
ANALYSIS:
55
Q3. On what occasions, do you often consume the Soft Drinks?
Feeling Thirsty
12% 18%
Parties /
Celebrations
25%
Without any
reason (just like
that)
Others
45%
ANALYSIS:
a) 18% of the respondents consume Soft Drinks when they feel thirsty.
b) 45% of the respondents consume Soft Drinks at the time of celebrations.
c) 25% of the respondents consume Soft Drinks without any reason.
d) 12% of the respondents consume Soft Drinks on other occasions.
56
Q4. On what occasions, do you often consume the Fruit Juices?
Feeling Thirsty
17%
27%
Parties /
Celebrations
Without any
reason (just like
32% that)
24% Others
ANALYSIS:
a) 17% of the respondents consume Fruit Juices when they feel thirsty.
b) 32% of the respondents consume Fruit Juices at the time of celebrations.
c) 24% of the respondents consume Fruit Juices without any reason.
d) 27% of the respondents consume Fruit Juices on other occasions.
57
Q5. What induces you to buy Soft Drinks?
35 32
30
30
26
No. of respondents
25
20
Series1
15
10 8
4
5
0
Price with Health Status Taste Variety
quantity Drink symbol
Factors
ANALYSIS:
58
Q6. What induces you to buy Fruit Juices?
45 40
40
35
No. of respondents
30
25 22
Series1
20 15
15 13
10
10
5
0
Price with Health Status Taste Variety
quantity Drink symbol
Factors
ANALYSIS:
59
Q7. If not a Soft Drink or a Fruit juice, which other beverage (other than water) tops your
mind?
Coffee 30 30%
Tea 25 25%
Shakes(Milk/Ice cream/Fruit) 8 8%
Lassi 12 12%
Sharbat 25 25%
Total 100 100%
Coffee
25%
30% Tea
Shakes(Milk/Ice-
cream/Fruit)
12% Lassi
ANALYSIS:
60
Q8. How do you rate canned juices as compared to fresh juices?
24%
Equivalent to fresh
40% juices
Have artificial
added flavor
Healthy with
preservatives
36%
ANALYSIS:
a) 24% of the respondents think canned juices are Equivalent to fresh juices.
b) 36% of the respondents think canned juices Have artificial added flavor.
c) 40% of the respondents think canned juices are Healthy but have
preservatives.
61
Q9. How do you view Soft Drinks?
As a health drink 4 4%
As a status symbol 8 8%
As an aid to put off thirst 55 55%
Any other 33 33%
Total 100 100%
60 55
50
No. of respondents
40
33
30 Series1
20
8
10 4
0
As a As a As an aid Any
health status to put off other
drink symbol thirst
ANALYSIS:
62
Q10. How do you view Fruit Juices?
45
40
40
35 32
No. of respondents
30
25
Series1
20
15
15 13
10
5
0
As a As a As an aid Any
health status to put off other
drink symbol thirst
ANALYSIS:
63
Q11. Do advertisements affect your purchases?
Yes 77 77%
No 23 23%
Total 100 100%
23%
Yes
No
77%
ANALYSIS:
64
CHAPTER 5
CONCLUSION, SUGGESTIONS AND LIMITATIONS
65
CONCLUSION
Following are the concluding points taken into consideration after the conduct of the
research study:
An important finding that emerged out of the survey was that 57%of people like to
have soft drinks while 43% go for juices.
Through the research it was conveyed that weekly consumption of soft drinks is
higher than the weekly consumption of juices.
A majority of the respondents consume soft drinks and fruit juices at the time of
parties & celebrations.
Most of the respondents consume soft drinks because of its taste and price.
As it is seen that canned Juices enjoys the largest market share though they do contain
some preservatives but majority of the respondents consider it healthy.
Most of the respondents view soft drinks as an aid to put off thirst and fruit juice as a
health drink.
Most of the respondents (77%) were of the strong view that advertisements affect
their purchases and the rest (23%) were not at all affected by the advertisements.
66
SUGGESTIONS
With the changing lifestyle, people have started becoming more health conscious.
Therefore, the fruit juice company’s should use appropriate marketing techniques
thereby reducing the demand for soft drink in the future.
The survey shows that parties/celebrations are the places where these drinks are
consumed mostly. The company’s must try to increase the sales by creating and
focusing on more utilities so that their product is used more frequently.
67
LIMITATIONS
68
ANNEXURE
QUESTIONNAIRE
PERSONAL DETAILS
Name - _________________
Age - 15-25 25-35 35-50
Gender - Male Female
Occupation - Student Housewife Business Service
69
Q4. On what occasions, do you often consume the Fruit juices?
a) Feeling Thirsty
b) Parties / Celebrations
c) Without any reason (just like that)
d) Others, please specify ________
Q5. What induces you to buy Soft Drinks?
a) Price with quantity ______
b) Health Drink ______
c) Status symbol ______
d) Taste ______
e) Variety ______
Q7. If not a Soft Drink or a Fruit juice, which other beverage (other than water) tops your
mind?
a) Coffee
b) Tea
c) Shakes (Milk/Ice-cream/Fruit)
d) Lassi
e) Sharbat
70
Q9. How do you view Soft Drinks?
a) As a health drink
b) As a status symbol
c) As an aid to put off thirst
d) Any other, please specify ________
Q12. Name any three brands of Fruit juices & Soft Drinks that are there in your mind at
present?
Soft Drinks Fruit Juices
______ ______
______ ______
______ ______
71
BIBLIOGRAPHY
http://en.wikipedia.org/wiki/Beverage
www.foodindustryindia.com
http://fnbnews.com/article/detarchive.asp?articleid=25105§ionid=3
http://www.foodindustryindia.com/newfood/detailnews.jsp?n=Xtazy,%20anot
her%20energy%20drink%20for%20the%20Indian%20market&id=598
http://fnbnews.com/redfr.asp?fn=/other/aboutus.asp&title=About%20Us#Foo
d&beverage
www.google.com
72