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    Genotype: Laci Laci Laci Laci Lacp Laci Laci Laco Laci Laci Lacp

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    Gia Hoàng
    1. The document provides information about gene expression in different E. coli genotypes under lactose present and lactose absent conditions. 2. It lists the expression of beta-galactosidase and permease enzymes for 4 E. coli genotypes with different combinations of lacI, lacP, lacO, lacZ, and lacY genes. 3. The second question asks to provide possible diploid genotypes that could produce different expression patterns of beta-galactosidase and permease under lactose present and lactose absent conditions.

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    Genotype: Laci Laci Laci Laci Lacp Laci Laci Laco Laci Laci Lacp

    Uploaded by

    Gia Hoàng
    230 views1 page
    1. The document provides information about gene expression in different E. coli genotypes under lactose present and lactose absent conditions. 2. It lists the expression of beta-galactosidase and permease enzymes for 4 E. coli genotypes with different combinations of lacI, lacP, lacO, lacZ, and lacY genes. 3. The second question asks to provide possible diploid genotypes that could produce different expression patterns of beta-galactosidase and permease under lactose present and lactose absent conditions.

    Original Title

    ASM1 SEM2

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    1. The document provides information about gene expression in different E. coli genotypes under lactose present and lactose absent conditions. 2. It lists the expression of beta-galactosidase and permease enzymes for 4 E. coli genotypes with different combinations of lacI, lacP, lacO, lacZ, and lacY genes. 3. The second question asks to provide possible diploid genotypes that could produce different expression patterns of beta-galactosidase and permease under lactose present and lactose absent conditions.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    230 views1 page

    Genotype: Laci Laci Laci Laci Lacp Laci Laci Laco Laci Laci Lacp

    Uploaded by

    Gia Hoàng
    1. The document provides information about gene expression in different E. coli genotypes under lactose present and lactose absent conditions. 2. It lists the expression of beta-galactosidase and permease enzymes for 4 E. coli genotypes with different combinations of lacI, lacP, lacO, lacZ, and lacY genes. 3. The second question asks to provide possible diploid genotypes that could produce different expression patterns of beta-galactosidase and permease under lactose present and lactose absent conditions.

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    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
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    ACADEMIC YEAR 2019 - SEM2 - GENETICS ASSIGNMENT 1- MAY

    FULL NAME: 1. STUDENT ID: 1.


    2. 2.
    3. 3.
    ---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
    QUESTION 1 (40 marks): Check the expression of indicated enzymes (USING “+” for expression, “-“ for no expression) in E.coli strains with the following
    genotypes

    β-galactosidase Permease
    Genotype
    Lactose present Lactose absent Lactose present Lactose absent

    lacI+ lacP+ lacOc lacZ - lacY+/lacI – lacP - lacO+ lacZ+ lacY+ _ _ + +

    lacIs lacP - lacO+ lacZ+ lacY -/lacI - lacP+ lacO+ lacZ – lacY+ _ _ + +

    lacI - lacP+ lacOc lacZ+ lacY+/lacI+ lacP - lacOc lacZ+ lacY+ _ _ _ _

    lacIs lacP - lacOc lacZ+ lacY+/lacI- lacP+ lacO+ lacZ+ lacY - + + _ _

    QUESTION 2 (60 marks): Provide 2 possible diploid genotypes that can produce the following products in EACH indicated condition.

    Lactose present Lactose absent


    β
    β
    Genotypes
    -galactosidas Permease Permease
    -galactosidase
    e

    G1: lacI+ lacP+ lacOc lacZ - lacY+/ lacIs lacP - lacO+ lacZ+ lacY -
    - + - +
    G2: lacIs lacP - lacO+ lacZ+ lacY -/ lacI - lacP+ lacO+ lacZ – lacY+

    G1: lacI+ lacP+ lacOc lacZ - lacY+/lacI- lacP+ lacO+ lacZ+ lacY -
    + - + +
    G2: lacI – lacP - lacO+ lacZ+ lacY+/lacI - lacP+ lacO+ lacZ – lacY+

    G1: lacI - lacP+ lacO+ lacZ – lacY+/lacI- lacP+ lacO+ lacZ+ lacY -
    + + - +
    G2: lacI - lacP+ lacO+ lacZ – lacY+/ lacI+ lacP+ lacOc lacZ - lacY+

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