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PowerSoil® DNA Isolation Kit

Catalog No. 12888-50

Quantity: 50 preps

Catalog No. 12888-100

Quantity: 100 preps

INSTRUCTION MANUAL

Version 07272016

Please recycle

www.mobio.com | T: 800-606-6246 | T: 760-929-9911 | technical@mobio.com

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TABLE OF CONTENTS

Introduction 5

Protocol Overview 5

Bead Beating Options 5

Equipment Required 8

Reagents Required But Not Included 8

Kit Contents & Storage 8

Precautions 9

Experienced User Protocol 10

Detailed Protocol 13

Vacuum Protocol using the PowerVac™ Manifold 17

Hints & Troubleshooting Guide 19

Technical Support 21

Trademarks 21

Contact Information 24

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INTRODUCTION
The PowerSoil® DNA Isolation Kit is comprised of a novel and proprietary method
for isolating genomic DNA from environmental samples utilizing our patented
Inhibitor Removal Technology® (IRT). The kit is intended for use with environmental
samples containing a high humic acid content including difficult soil types such as
compost, sediment, and manure. Other more common soil types have also been
used successfully with this kit. The isolated DNA has a high level of purity allowing
for more successful PCR amplification of organisms from the sample. PCR analysis
has been performed to detect a variety of organisms including bacteria (e.g. Bacillus
subtilis, Bacillus anthracis), fungi (e.g. yeasts, molds), algae and Actinomycetes
(e.g. Streptomyces).

PROTOCOL OVERVIEW
The PowerSoil® DNA Isolation Kit is effective at removing PCR inhibitors from even
the most difficult soil types. Environmental samples are added to a bead beating
tube for rapid and thorough homogenization. Cell lysis occurs by mechanical and
chemical methods. Total genomic DNA is captured on a silica membrane in a spin
column format. DNA is then washed and eluted from the membrane. DNA is then
ready for PCR analysis and other downstream applications.

BEAD BEATING OPTIONS

The PowerSoil® DNA Isolation Kit does not require homogenization using a high
velocity bead beater. However, if the microorganism of interest requires stronger
homogenization than provided by a vortex, or if using a bead beater is desired, the
PowerSoil® DNA Isolation Kit may be used in conjunction with the PowerLyzer® 24
homogenizer. MO BIO now offers the PowerLyzer® PowerSoil® DNA Isolation Kit
(cat# 12855-50) with a Bead Tube suitable for high powered bead beating of soil.
For more information about these products, or for references using the PowerSoil®
DNA Isolation Kit with a FastPrep® instrument, please contact Technical Service at
1-800-606-6246 or technical@mobio.com.

Additional information can be found at www.mobio.com/blog in the following articles:

https://mobio.com/blog/cat/technical-tips/post/Molecular_Biology_of_Soil_an_
introduction/

https://mobio.com/blog/cat/technical-tips/post/Molecular_Biology_of_Soil_
DNA_Isolation_Part_I/

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PowerLyzer® 24 Bench Top Bead-Based Homogenizer
The PowerLyzer® 24 Bench Top Bead-Based Homogenizer is a bead beating instrument
uniquely designed for the most efficient and complete lysis and homogenization of
any biological sample. In as little as 30 seconds, the PowerLyzer® 24 homogenizer
is capable of processing up to 24 samples in 2 ml tubes. With true “hands-free”
operation, the downtime associated with manipulating samples through multiple
cycles is eliminated. Even the toughest and most difficult samples such as pine
needles, seeds, spores, fungal mats, and clay soils are easily and effectively lysed.
For more information and protocols, call technical service.

PowerLyzer® 24
Bench Top Bead-Based Homogenizer
Catalog#13155
www.mobio.com/powerlyzer

High Throughput Options

MO BIO offers a vacuum based protocol for faster processing without
centrifugation for the DNA binding and column washing steps for Spin Filters.
The MO BIO PowerVac™ Manifold allows for processing of up to 20 spin filter
preps at a time using the PowerVac™ Mini Spin Filter Adapters. For additional
high throughput options MO BIO offers the PowerSoil®-htp 96 Well Soil DNA
Isolation Kit for processing up to 2 x 96 samples using a centrifuge capable of
spinning two 96 Well Blocks stacked (13 cm x 8 cm x 5.5 cm) at 2500 x g.
For 96 well homogenization of soil, MO BIO offers the 96 Well Plate Shaker
and Plate Adapter Set (MO BIO Catalog# 11996 & 11990, respectively.)

This kit is for research purposes only. Not for diagnostic use.

Other Related Products Catalog # Quantity

PowerMax® Soil DNA Isolation Kit 12988-10 10 preps

PowerSoil®-htp 96 Well Soil DNA Isolation Kit 12955-4 4 x 96 preps

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EQUIPMENT REQUIRED
FF Microcentrifuge (10,000 x g)
FF Pipettors (50 μl - 500 μl)
FF Vortex-Genie® 2 Vortex (MO BIO Catalog# 13111-V or 13111-V-220)
FF Vortex Adapter (MO BIO Catalog # 13000-V1-24)

REAGENTS REQUIRED BUT NOT INCLUDED

FF 100% ethanol (for the PowerVac™ Manifold protocol only)

KIT CONTENTS

Kit Catalog # Kit Catalog #

Component Catalog # Amount Catalog # Amount
PowerBead Tubes
12888-50-PBT 50 12888-100-PBT 100
(contain 750 µl solution)
PowerSoil® Solution C1 12888-50-1 3.3 ml 12888-100-1 6.6 ml
PowerSoil Solution C2
® 12888-50-2 14 ml 12888-100-2 28 ml
PowerSoil Solution C3
® 12888-50-3 11 ml 12888-100-3 22 ml
PowerSoil Solution C4
® 12888-50-4 72 ml 12888-100-4 144 ml
PowerSoil® Solution C5 12888-50-5 30 ml 12888-100-5 60 ml
PowerSoil® Solution C6 12888-50-6 6 ml 12888-100-6 12 ml
PowerSoil Spin Filters
®
12888-50-SF 50 12888-100-SF 100
(units in 2 ml tubes)
PowerSoil® 2 ml
12888-50-T 200 12888-100-T 400
Collection Tubes

KIT STORAGE
Kit reagents and components should be stored at room temperature (15-30°C).

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PRECAUTIONS
Please wear gloves when using this product. Avoid all skin contact with
reagents in this kit. In case of contact, wash thoroughly with water. Do not
ingest. See Material Safety Data Sheets for emergency procedures in case
of accidental ingestion or contact. All SDS information is available upon
request (760-929-9911) or on our web site at www.mobio.com. Reagents
labeled flammable should be kept away from open flames and sparks.

WARNING: Solution C5 contains ethanol. It is flammable. Do not use bleach to

clean the inside of the PowerVac™ Manifold or to rinse the PowerVac™ Mini Spin
Filter Adapters when attached to the manifold.

IMPORTANT NOTE FOR USE: Make sure the 2 ml PowerBead Tubes rotate freely in
your centrifuge without rubbing. Shake to mix Solution C4 before use.

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EXPERIENCED USER PROTOCOL
PowerSoil® DNA Isolation Kit
Catalog No. 12888-50 & 12888-100

Please wear gloves at all times

1. To the PowerBead Tubes provided, add 0.25 grams of soil sample.

2. Gently vortex to mix.
3. Check Solution C1. If Solution C1 is precipitated, heat solution to 60°C until
dissolved before use.
4. Add 60 μl of Solution C1 and invert several times or vortex briefly.
5. Secure PowerBead Tubes horizontally using the MO BIO Vortex Adapter
tube holder for the vortex (MO BIO Catalog# 13000-V1-24) or secure tubes
horizontally on a flat-bed vortex pad with tape. Vortex at maximum speed for 10
minutes.

Note
If you are using the 24 place Vortex Adapter for more than
12 preps, increase the vortex time by 5-10 minutes.

6. Make sure the PowerBead Tubes rotate freely in your centrifuge without
rubbing. Centrifuge tubes at 10,000 x g for 30 seconds at room temperature.
CAUTION: Be sure not to exceed 10,000 x g or tubes may break.
7. Transfer the supernatant to a clean 2 ml Collection Tube (provided).

Note
Expect between 400 to 500 μl of supernatant. Supernatant
may still contain some soil particles.

8. Add 250 μl of Solution C2 and vortex for 5 seconds. Incubate at 4°C for 5
minutes.
9. Centrifuge the tubes at room temperature for 1 minute at 10,000 x g.
10. Avoiding the pellet, transfer up to, but no more than, 600 μl of supernatant
to a clean 2 ml Collection Tube (provided).
11. Add 200 μl of Solution C3 and vortex briefly. Incubate at 4°C for 5 minutes.
12. Centrifuge the tubes at room temperature for 1 minute at 10,000 x g.
13. Avoiding the pellet, transfer up to, but no more than, 750 μl of supernatant
into a clean 2 ml Collection Tube (provided).
14. Shake to mix Solution C4 before use. Add 1200 μl of Solution C4 to the
supernatant and vortex for 5 seconds.

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15. Load approximately 675 μl onto a Spin Filter and centrifuge at 10,000
x g for 1 minute at room temperature. Discard the flow through and add an
additional 675 μl of supernatant to the Spin Filter and centrifuge at 10,000 x g
for 1 minute at room temperature. Load the remaining supernatant onto the Spin
Filter and centrifuge at 10,000 x g for 1 minute at room temperature.

Note
A total of three loads for each sample processed are
required.

16. Add 500 μl of Solution C5 and centrifuge at room temperature for 30

seconds at 10,000 x g.
17. Discard the flow through.
18. Centrifuge again at room temperature for 1 minute at 10,000 x g.
19. Carefully place spin filter in a clean 2 ml Collection Tube (provided). Avoid
splashing any Solution C5 onto the Spin Filter.
20. Add 100 μl of Solution C6 to the center of the white filter membrane.
Alternatively, sterile DNA-Free PCR Grade Water may be used for elution from
the silica Spin Filter membrane at this step (MO BIO Catalog# 17000-10).
21. Centrifuge at room temperature for 30 seconds at 10,000 x g.
22. Discard the Spin Filter. The DNA in the tube is now ready for any downstream
application. No further steps are required.

We recommend storing DNA frozen (-20° to -80°C). Solution C6 contains no

EDTA. To concentrate the DNA see the Hints & Troubleshooting Guide.

Thank you for choosing the PowerSoil® DNA Isolation Kit!

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DETAILED USER PROTOCOL (DESCRIBES WHAT IS HAPPENING AT EACH STEP)
PowerSoil® DNA Isolation Kit
Catalog No. 12888-50 & 12888-100

Please wear gloves at all times

1. To the PowerBead Tubes provided, add 0.25 grams of soil sample.
What’s happening: After your sample has been loaded into the PowerBead
Tube, the next step is a homogenization and lysis procedure. The PowerBead
Tube contains a buffer that will (a) help disperse the soil particles, (b) begin
to dissolve humic acids and (c) protect nucleic acids from degradation.
2. Gently vortex to mix.
What’s happening: Gentle vortexing mixes the components in the PowerBead
Tube and begins to disperse the sample in the PowerBead Solution.
3. Check Solution C1. If Solution C1 is precipitated, heat solution to 60°C until
the precipitate has dissolved before use.
What’s happening: Solution C1 contains SDS and other disruption agents
required for complete cell lysis. In addition to aiding in cell lysis, SDS is an
anionic detergent that breaks down fatty acids and lipids associated with
the cell membrane of several organisms. If it gets cold, it will form a white
precipitate in the bottle. Heating to 60°C will dissolve the SDS and will not
harm the SDS or the other disruption agents. Solution C1 can be used while
it is still warm.
4. Add 60 μl of Solution C1 and invert several times or vortex briefly.
5. Secure PowerBead Tubes horizontally using the MO BIO Vortex Adapter
tube holder for the vortex (MO BIO Catalog# 13000-V1-24) or secure tubes
horizontally on a flat-bed vortex pad with tape. Vortex at maximum speed for 10
minutes.

Note
Note: If you are using the 24 place Vortex Adapter for more
than 12 preps, increase the vortex time by 5-10 minutes.

Note
The vortexing step is critical for complete homogenization
and cell lysis. Cells are lysed by a combination of chemical
agents from steps 1-4 and mechanical shaking introduced
at this step. By randomly shaking the beads in the presence
of disruption agents, collision of the beads with microbial
cells will cause the cells to break open.

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 What’s happening: The MO BIO Vortex Adapter is designed to be a simple
platform to facilitate keeping the tubes tightly attached to the vortex. It
should be noted that although you can attach tubes with tape, often the tape
becomes loose and not all tubes will shake evenly or efficiently. This may
lead to inconsistent results or lower yields. Therefore, the use of the MO BIO
Vortex Adapter is a highly recommended and cost effective way to obtain
maximum DNA yields.
6. Make sure the PowerBead Tubes rotate freely in your centrifuge without
rubbing. Centrifuge tubes at 10,000 x g for 30 seconds at room temperature.
CAUTION: Be sure not to exceed 10,000 x g or tubes may break.
7. Transfer the supernatant to a clean 2 ml Collection Tube (provided).

Note
Expect between 400 to 500 μl of supernatant at this step.
The exact recovered volume depends on the absorbency of
your starting material and is not critical for the procedure to
be effective. The supernatant may be dark in appearance
and still contain some soil particles. The presence of carry
over soil or a dark color in the mixture is expected in many
soil types at this step. Subsequent steps in the protocol will
remove both carry over soil and coloration of the mixture.

8. Add 250 μl of Solution C2 and vortex for 5 seconds. Incubate at 4°C for 5
minutes.
What’s happening: Solution C2 is patented Inhibitor Removal Technology®
(IRT). It contains a reagent to precipitate non-DNA organic and inorganic
material including humic substances, cell debris, and proteins. It is important
to remove contaminating organic and inorganic matter that may reduce
DNA purity and inhibit downstream DNA applications.
9. Centrifuge the tubes at room temperature for 1 minute at 10,000 x g.
10. Avoiding the pellet, transfer up to 600 μl of supernatant to a clean 2 ml
Collection Tube (provided).
What’s happening: The pellet at this point contains non-DNA organic and
inorganic material including humic acid, cell debris, and proteins. For the
best DNA yields, and quality, avoid transferring any of the pellet.
11. Add 200 μl of Solution C3 and vortex briefly. Incubate at 4°C for 5 minutes.
What’s happening: Solution C3 is patented Inhibitor Removal Technology®
(IRT) and is a second reagent to precipitate additional non-DNA organic
and inorganic material including humic acid, cell debris, and proteins. It is
important to remove contaminating organic and inorganic matter that may
reduce DNA purity and inhibit downstream DNA applications.

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12. Centrifuge the tubes at room temperature for 1 minute at 10,000 x g.
13. Transfer up to 750 μl of supernatant to a clean 2 ml Collection Tube
(provided).
What’s happening: The pellet at this point contains additional non-DNA
organic and inorganic material including humic acid, cell debris, and
proteins. For the best DNA yields, and quality, avoid transferring any of
the pellet.
14. Shake to mix Solution C4 before use. Add 1.2 ml of Solution C4 to the
supernatant (be careful solution doesn’t exceed rim of tube) and vortex for 5
seconds.
What’s happening: Solution C4 is a high concentration salt solution. Since
DNA binds tightly to silica at high salt concentrations, this will adjust the
DNA solution salt concentrations to allow binding of DNA, but not non-DNA
organic and inorganic material that may still be present at low levels, to the
Spin Filters.
15. Load approximately 675 μl onto a Spin Filter and centrifuge at 10,000
x g for 1 minute at room temperature. Discard the flow through and add an
additional 675 μl of supernatant to the Spin Filter and centrifuge at 10,000 x g
for 1 minute at room temperature. Load the remaining supernatant onto the Spin
Filter and centrifuge at 10,000 x g for 1 minute at room temperature.

Note
A total of three loads for each sample processed are
required.

What’s happening: DNA is selectively bound to the silica membrane in the

Spin Filter device in the high salt solution. Contaminants pass through the
filter membrane, leaving only DNA bound to the membrane.
16. Add 500 μl of Solution C5 and centrifuge at room temperature for 30
seconds at 10,000 x g.
What’s happening: Solution C5 is an ethanol based wash solution used
to further clean the DNA that is bound to the silica filter membrane in the
Spin Filter. This wash solution removes residual salt, humic acid, and other
contaminants while allowing the DNA to stay bound to the silica membrane.
17. Discard the flow through from the 2 ml Collection Tube.
What’s happening: This flow through fraction is just non-DNA organic
and inorganic waste removed from the silica Spin Filter membrane by the
ethanol wash solution.

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18. Centrifuge at room temperature for 1 minute at 10,000 x g.
What’s happening: This second spin removes residual Solution C5
(ethanol wash solution). It is critical to remove all traces of wash
solution because the ethanol in Solution C5 can interfere with many
downstream DNA applications such as PCR, restriction digests, and
gel electrophoresis.
19. Carefully place Spin Filter in a clean 2 ml Collection Tube (provided).
Avoid splashing any Solution C5 onto the Spin Filter.

Note
It is important to avoid any traces of the ethanol based wash
solution.

20. Add 100 μl of Solution C6 to the center of the white filter membrane.

Note
Placing the Solution C6 (sterile elution buffer) in the
center of the small white membrane will make sure the
entire membrane is wetted. This will result in a more
efficient and complete release of the DNA from the
silica Spin Filter membrane. As Solution C6 (elution
buffer) passes through the silica membrane, DNA that
was bound in the presence of high salt is selectively
released by Solution C6 (10 mM Tris) which lacks salt.

Alternatively, sterile DNA-Free PCR Grade Water may be

used for DNA elution from the silica Spin Filter membrane
at this step (MO BIO Catalog# 17000-10). Solution C6
contains no EDTA. If DNA degradation is a concern, Sterile
TE may also be used instead of Solution C6 for elution of
DNA from the Spin Filter.

21. Centrifuge at room temperature for 30 seconds at 10,000 x g.

22. Discard the Spin Filter. The DNA in the tube is now ready for any downstream
application. No further steps are required.

We recommend storing DNA frozen (-20° to -80°C). Solution C6 does not

contain any EDTA. To concentrate DNA see the Hints & Troubleshooting Guide.

Thank you for choosing the PowerSoil® DNA Isolation Kit!

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VACUUM PROTOCOL USING THE POWERVAC™ MANIFOLD
Please wear gloves at all times

For each sample lysate, use one Spin Filter column. Keep the Spin Filter in the
attached 2 ml Collection Tube and continue using the Collection Tube as a
Spin Filter holder until needed for the Vacuum Manifold Protocol. Label each
Collection Tube top and Spin Filter column to maintain sample identity. If the
Spin Filter becomes clogged during the vacuum procedure, you can switch to the
procedure for purification of the DNA by centrifugation.
You will need to provide 100% ethanol for step 4 of this protocol.
1. For each prep, attach one aluminum PowerVac™ Mini Spin Filter Adapter
(MO BIO Catalog# 11992-20) into the Luer-Lok® fitting of one port in the
manifold. Gently press a Spin Filter column into the PowerVac™ Mini Spin Filter
Adapter until snugly in place. Ensure that all unused ports of the vacuum manifold
are closed.

Note
Aluminum PowerVac™ Mini Spin Filter Adapters are
reusable.

2. Transfer 650 μl of prepared sample lysate (from step 14) to the Spin Filter
column.
3. Turn on the vacuum source and open the stopcock of the port. Hold the
tube in place when opening the stopcock to keep the spin filter steady. Allow the
lysate to pass through the Spin Filter column. After the lysate has passed through
the column completely, load again with the next 650 μl of lysate. Continue until
all of the lysate has been loaded onto the Spin Filter column. Close the one-way
Luer-Lok® stopcock of that port.

Note
If Spin Filter Columns are filtering slowly, close the ports
to samples that have completed filtering to increase the
pressure to the other columns.

4. Load 800 μl of 100% ethanol into the Spin Filter so that it completely fills the
column. Open the stopcock while holding the column steady. Allow the ethanol
to pass through the column completely. Close the stopcock.
5. Add 500 μl of Solution C5 to each Spin Filter. Open the Luer-Lok® stopcock
and apply a vacuum until Solution C5 has passed through the Spin Filter
completely. Continue to pull a vacuum for another minute to dry the membrane.
Close each port.
6. Turn off the vacuum source and open an unused port to vent the manifold.
If all 20 ports are in use, break the vacuum at the source. Make certain that all
vacuum pressure is released before performing the next step. It is important to
turn off the vacuum at the source to prevent backflow into the columns.

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7. Remove the Spin Filter column and place in the original labeled 2 ml
Collection Tube. Place into the centrifuge and spin at 13,000 × g for 1 minute to
completely dry the membrane.
8. Transfer the Spin Filter column to a new 2 ml Collection Tube and add 100
μl of Solution C6 to the center of the white filter membrane. Alternatively, sterile
DNA-Free PCR Grade Water may be used for elution from the silica Spin Filter
membrane at this step (MO BIO Catalog # 17000-10).
9. Centrifuge at room temperature for 30 seconds at 10,000 x g.
10. Discard the Spin Filter column. The DNA in the tube is now ready for any
downstream application. No further steps are required.
We recommend storing DNA frozen (-20° to -80°C). Solution C6 contains no
EDTA. To concentrate the DNA see the Hints & Troubleshooting Guide.

Thank you for choosing the PowerSoil® DNA Isolation Kit!

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HINTS AND TROUBLESHOOTING GUIDE
Amount of Soil to Process
This kit is designed to process 0.25 grams of soil. For inquiries regarding the use
of larger sample amounts, please contact technical support for suggestions. For wet
soils, see information under “Wet Soil Sample” below.

Wet Soil Sample

If soil sample is high in water content, remove contents from PowerBead Tube (beads
and solution) and transfer into another sterile microcentrifuge tube (not provided).
Add soil sample to PowerBead Tube and centrifuge at room temperature for 30
seconds at 10,000 x g. Remove as much liquid as possible with a pipet tip. Add
beads and bead solution back to PowerBead Tube and follow protocol starting at
step 2.

If DNA Does Not Amplify

•  Make sure to check DNA yields by gel electrophoresis or spectrophotometer
reading. An excess amount of DNA will inhibit a PCR reaction.
•  Diluting the template DNA should not be necessary with DNA isolated with the
PowerSoil® DNA Isolation Kit; however, it should still be attempted.
•  If DNA will still not amplify after trying the steps above, then PCR optimization
(changing reaction conditions and primer choice) may be needed.

Eluted DNA Sample Is Brown

We have not observed any coloration in DNA isolated using the PowerSoil® DNA
Isolation Kit. If you observe coloration in your samples, please contact technical
support for suggestions.

Alternative Lysis Methods

•  After adding Solution C1, vortex 3-4 seconds, then heat to 70°C for 5 minutes.
Vortex 3-4 seconds. Heat another 5 minutes. Vortex 3-4 seconds. This alternative
procedure will reduce shearing but may also reduce yield.
•  If cells are difficult to lyse, a 10 minute incubation at 70°C, after adding Solution
C1, can be performed. Follow by continuing with protocol step 5.

Concentrating the DNA

The final volume of eluted DNA will be 100 μl. The DNA may be concentrated by
adding 4 μl of 5 M NaCl and inverting 3-5 times to mix. Next, add 200 μl of 100%
cold ethanol and invert 3-5 times to mix. Centrifuge at 10,000 x g for 5 minutes
at room temperature. Decant all liquid. Remove residual ethanol in a speed vac,
dessicator, or air dry. Resuspend precipitated DNA in sterile water or sterile 10 mM
Tris.

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DNA Floats Out of Well When Loaded on a Gel
This usually occurs because residual Solution C5 remains in the final sample. Prevent
this by being careful in step 19 not to transfer liquid onto the bottom of the spin filter
basket. Ethanol precipitation (described in “Concentrating the DNA”) is the best
way to remove residual Solution C5.

Storing DNA
DNA is eluted in Solution C6 (10 mM Tris) and must be stored at -20° to -80°C to
prevent degradation. DNA can be eluted in TE without loss, but the EDTA may inhibit
downstream reactions such as PCR and automated sequencing. DNA may also be
eluted with sterile DNA-Free PCR Grade Water (MO BIO Catalog# 17000-10).

Cleaning of the PowerVac™ Mini Spin Filter Adapters

It is recommended to rinse the PowerVac™ Mini Spin Filter Adapters promptly after
use to avoid salt build up. To clean the PowerVac™ Mini Spin Filter Adapters, rinse
each adapter with DI water followed by 70% ethanol and flush into the manifold
base. Alternatively, remove the adapters and wash in laboratory detergent and DI
water. PowerVac™ Mini Spin Filter Adapters may be autoclaved.

Do not use bleach to clean the PowerVac™ Mini Spin Filter Adapters while attached
to the PowerVac™ Manifold. Bleach should never be mixed with solutions containing
guanidine and should not be used to clean the PowerVac™ Manifold. For more
information on cleaning the PowerVac™ Manifold, please refer to the PowerVac™
Manifold manual.

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TECHNICAL SUPPORT Committed to resolving your technical
Phone: Toll Free 800-606-6246, questions promptly, our technical support
team is trained to work with you to rapidly
or 760-929-9911
and effectively trouble shoots any issues.
Email: technical@mobio.com We commit to providing you with relevant
Mail: MO BIO Laboratories, Inc., online support resources that help you
complete your research projects.
2746 Loker Ave West, Carlsbad,
CA 92010

Frequently Asked Questions:

https://mobio.com/faq

Trademarks
Inhibitor Removal Technology® (IRT) is a registered trademark of MO BIO Laboratories, Inc. and
is covered by patents.
For other Trademarks and Limited Use Label License information go to:
www.mobio.com/lull-tm

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Corporate Headquarters
MO BIO Laboratories, Inc.
2746 Loker Avenue West
Carlsbad, CA 92010

Technical Support:
Toll Free 800-606-6246, or 760-929-9911
Email: technical@mobio.com

Ordering Information:
Toll Free 800-606-6246, or 760-929-9911
Fax: 760-929-0109
Email: orders@mobio.com

For the distributor nearest you, visit our website at www.mobio.com/distributors

World Leader in Soil DNA & RNA Isolation®. © 2014 MO BIO Laboratories, Inc. All Rights Reserved

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