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    Cell Communication: Sally Roden Wcsu Bio 300 Dr. Dye Spring 2018

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    This laboratory procedure observed the life cycle stages of the organism Dictyostelium discoideum from spores. When food is plentiful, D. discoideum lives as individual cells reproducing through binary fission. When food is scarce, the cells aggregate using cyclic AMP signaling and undergo morphological changes to form a multicellular pseudoplasmodium and ultimately a sorocarp containing spores. The life cycle stages observed included the Mexican hat, pseudoplasmodium, and sorocarp. It was difficult to prepare wet mount slides of the pseudoplasmodium and sorocarp due to damage from needles. The sorocarp contained two cell types - elongated spore cells and larger cube-

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    Cell Communication: Sally Roden Wcsu Bio 300 Dr. Dye Spring 2018

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    sally r
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    This laboratory procedure observed the life cycle stages of the organism Dictyostelium discoideum from spores. When food is plentiful, D. discoideum lives as individual cells reproducing through binary fission. When food is scarce, the cells aggregate using cyclic AMP signaling and undergo morphological changes to form a multicellular pseudoplasmodium and ultimately a sorocarp containing spores. The life cycle stages observed included the Mexican hat, pseudoplasmodium, and sorocarp. It was difficult to prepare wet mount slides of the pseudoplasmodium and sorocarp due to damage from needles. The sorocarp contained two cell types - elongated spore cells and larger cube-

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    Cell Communication

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    This laboratory procedure observed the life cycle stages of the organism Dictyostelium discoideum from spores. When food is plentiful, D. discoideum lives as individual cells reproducing through binary fission. When food is scarce, the cells aggregate using cyclic AMP signaling and undergo morphological changes to form a multicellular pseudoplasmodium and ultimately a sorocarp containing spores. The life cycle stages observed included the Mexican hat, pseudoplasmodium, and sorocarp. It was difficult to prepare wet mount slides of the pseudoplasmodium and sorocarp due to damage from needles. The sorocarp contained two cell types - elongated spore cells and larger cube-

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    © All Rights Reserved
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    28 views4 pages

    Cell Communication: Sally Roden Wcsu Bio 300 Dr. Dye Spring 2018

    Uploaded by

    sally r
    This laboratory procedure observed the life cycle stages of the organism Dictyostelium discoideum from spores. When food is plentiful, D. discoideum lives as individual cells reproducing through binary fission. When food is scarce, the cells aggregate using cyclic AMP signaling and undergo morphological changes to form a multicellular pseudoplasmodium and ultimately a sorocarp containing spores. The life cycle stages observed included the Mexican hat, pseudoplasmodium, and sorocarp. It was difficult to prepare wet mount slides of the pseudoplasmodium and sorocarp due to damage from needles. The sorocarp contained two cell types - elongated spore cells and larger cube-

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    Cell Communication

    Sally Roden
    WCSU BIO 300
    Dr. Dye
    Spring 2018
    Introduction:
    This laboratory procedure is designed to help students learn about cell communication
    by making a culture of Dictyostelium discoideum from spores and observing the various life
    cycle stages of the organism. In addition, The procedure also included preparing slides of
    pseudoplasmodium and sorocarp and observing them under the microscope and sketching the
    stages of the life cycle identified. Dictyostelium dicoideum has the ability to live as individual
    cells and reproduce by binary fission when conditions are ideal. The organism can also initiate a
    cooperative phase of its life cycle when its food supply is decreased. The cells will aggregate
    together through the use of cyclic AMP that leads to morphological, biological and molecular
    changes that result in the formation of the fruiting body. The spores are able to act as a resting
    phase until food becomes available and the spores geminate resulting in amoeba and
    continuing the life cycle.

    Materials and Methods:


    Refer to page 43 in the Cell Biology Laboratory Manual (Dye, 2018).

    Observations:

    The life cycle stages of Dictyostelium observed included the Mexican hat, pseudoplasmodium
    (the slug), and the sorocarp. We had difficulty in obtaining a pseudoplasodium (slug) and
    sorocarp for the wet mount slides. Each time we had a suitable specimen selected the needle
    would sever the specimen or force it into the agar. We were unable to make the wet mount
    slides and had to view another group’s slides for observation. The cells of the sorocarp showed
    two different types of cells. The sorus contained the spore cells which were elongated and pill
    (bacilli) shaped. The stalk (sorophore) had cell that were a bit larger and more cube shaped.
    Sketches/Data:

    Discussion:

    Dictyostelium is an organism of interest for biomedical researchers and cell biologists for a
    number of reasons. First, Dictyostelium demonstrates cell communication and cell
    differentiation. It has a 24 hour turnover time, and the organism uses cAMP as part of
    chemotaxis in its intracellular communication. The Dictyostelium can be manipulated in its life
    cycle patterns by removing its food source and inducing a chemotaxis response. This lab gave
    us insight into the life cycle of Dictyostelium. It was fascinating to learn how even after
    dissection the organism has the ability to continue on in its life cycle as separate (but smaller)
    individuals able to regulate for the missing pieces. Learning how the cells can go from one type
    as a pseudoplasmodium and differentiate into the two types of cells in the sorocarp (the stalk
    and soros) was very interesting.

    References:

    Dye, Frank Ph.D., Cell Biology Laboratory Manual. WCSU. 2018.

    www.dictybase.org

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