Instruction Manual

HI 96715C
Ammonia Medium Range ISM

www.hannainst.com

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Dear Customer,
Thank you for choosing a Hanna product. This manual will provide you with the necessary PRELIMINARY EXAMINATION
information for the correct use of the instrument. Please read it carefully before using the meter. If
you need additional technical information, do not hesitate to e-mail us at tech@hannainst.com. Please examine this product carefully. Make sure that the instrument is not damaged. If any
damage occurred during shipment, please notify your Dealer.
Each HI 96715 Ion Selective Meter is supplied complete with:
• Two Sample Cuvettes and Caps
TABLE OF CONTENTS • Two CAL CHECKTM standard cuvettes HI 96715KIT
• 9V Battery
PRELIMINARY EXAMINATION ................................................................................................ 3
• Tissue for wiping cuvettes
GENERAL DESCRIPTION ....................................................................................................... 4
• Instrument quality certificate
ABBREVIATIONS .................................................................................................................. 4
SPECIFICATIONS ................................................................................................................... 5 • Instruction Manual
PRECISION AND ACCURACY .................................................................................................. 5 • Rigid carrying case
PRINCIPLE OF OPERATION ................................................................................................... 6
FUNCTIONAL DESCRIPTION .................................................................................................. 7
ERRORS AND WARNINGS .................................................................................................... 9
GENERAL TIPS FOR AN ACCURATE MEASUREMENT ............................................................. 11
STARTUP ........................................................................................................................... 13
MEASUREMENT PROCEDURE ............................................................................................. 13
VALIDATION PROCEDURE ................................................................................................... 15
CALIBRATION PROCEDURE ................................................................................................. 17
GLP ................................................................................................................................... 20
BATTERY MANAGEMENT .................................................................................................... 21
BATTERY REPLACEMENT .................................................................................................... 22
ACCESSORIES ..................................................................................................................... 22
WARRANTY ........................................................................................................................ 23

Note:Save all packing material until you are sure that the instrument works correctly. Any
defective item must be returned in its original packing.
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895 , USA.

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 GENERAL DESCRIPTION SPECIFICATIONS

The HI 96715 is an auto diagnostic portable microprocessor meter that benefits from Hanna’s Range 0.00 to 9.99 mg/L (as NH3-N)
years of experience as a manufacturer of analytical instruments. It has the advanced optical Resolution 0.01 mg/L for measurement
system based on a special tungsten lamp and a narrow band interference filter that allows most 0.1 mg/L for calibration and validation
accurate and repeatable readings. All instruments are factory calibrated and the electronic and Accuracy ±0.05 mg/L ±5% of reading @ 25°C
optical design minimizes the need of frequent calibration. Typical EMC Deviation ±0.01 mg/L
With the powerful CAL CHECKTM validation function, you are able to validate good performance Light Source Light Emitting Diode
of your instrument at any time. The validation procedure is extremely user friendly. Just use the Light Detector Silicon Photocell with narrow band interference filter @ 466 nm
exclusive HANNA ready-made, NIST traceable standards to verify the performance of the Method Adaptation of the ASTM Manual of Water and Environmental Technology,
D1426-93, Nessler Method. The reaction between ammonia and the
instrument and recalibrate if necessary. reagents causes a yellow tint in the sample.
The instrument is splash waterproof and the LED and filter units are protected from dust or dirt Environment 0 to 50°C (32 to 122°F); max 95% RH non-condensing
by a transparent cup. This makes the instruments fulfill field applications. Display messages aid Battery Type 1 x 9 volt
the user in routine operation. The meter has an auto-shut off feature that will turn off the Auto-Shut off After 10’ of non-use in measurement mode;
instrument after 10 minutes of non use in measurement mode or after 1 hour if left in after 1 hour of non-use in calibration mode;
calibration mode. with last reading reminder.
The meter uses an exclusive positive-locking system to ensure that the cuvette is in the same Dimensions 192 x 104 x 69 mm (7.6 x 4.1 x 2.7”)
position every time it is placed into the measurement cell. It is designed to fit a cuvette with a Weight 360 g (12.7 oz.).
larger neck making it easier to add both sample and reagents. The cuvette is made from special REQUIRED REAGENTS
optical glass to obtain best results. Code Description Quantity/test
The HI 96715 meter measures the ammonia nitrogen (NH3-N) content in water samples in the HI 93715A-0 First Reagent 4 drops (6 drops in seawater)
0.00 to 9.99 mg/L (ppm) range. The method is an adaptation from ASTM Manual of Water and HI 93715B-0 Second Reagent 4 drops (10 drops in seawater)
Environmental Technology, D1426-93, Nessler Method.
PRECISION AND ACCURACY
The reagents are liquid form and are supplied in bottles. The amount of reagent is precisely
dosed to ensure the maximum repeatability. Precision is how closely repeated measurements
agree with each other. Precision is usually
expressed as standard deviation (SD).
ABBREVIATIONS Accuracy is defined as the nearness of a test
result to the true value.
Although good precision suggests good accuracy,
°C: degree Celsius
precise results can be inaccurate. The figure
°F: degree Fahrenheit explains these definitions.
mg/L: milligrams per liter. mg/L is equivalent to ppm (parts per million) In a laboratory using a standard solution of 6.00
mL: milliliter mg/L ammonia nitrogen (NH 3-N) and a
LED: Light Emitting Diode representative lot of reagents, an operator
LCD: Liquid Crystal Display obtained with a single instrument a standard
ASTM: American Society for Testing and Materilas deviation of 0.12 mg/L.
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 A microprocessor controlled LED emits radiation which is first optically conditioned and beamed
PRINCIPLE OF OPERATION
to the sample contained in the cuvette. The optical path is fixed by the diameter of the cuvette.
Then the light is spectrally filtered to a narrow spectral bandwidth, to obtain a light beam of
Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and intensity Io or I.
matter. When a light beam crosses a substance, some of the radiation may be absorbed by
The photoelectric cell collects the radiation I that is not absorbed by the sample and converts
atoms, molecules or crystal lattices.
it into an electric current, producing a potential in the mV range.
If pure absorption occurs, the fraction of light absorbed depends both on the optical path length
The microprocessor uses this potential to convert the incoming value into the desired measuring
through the matter and on the physical-chemical characteristics of the substance according to the
unit and to display it on the LCD.
Lambert-Beer Law:
The measurement process is carried out in two phases: first the meter is zeroed and then the
-log I/Io = ελ c d
actual measurement is performed.
or
A = ελ c d The cuvette has a very important role because it is an optical element and thus requires
particular attention. It is important that both, the measurement and the calibration (zeroing)
Where: cuvettes, are optically identical to provide the same measurement conditions. Whenever possible
-log I/Io = Absorbance (A) use the same cuvette for both. It is necessary that the surface of the cuvette is clean and not
Io = intensity of incident light beam scratched. This to avoid measurement interference due to unwanted reflection and absorption of
I = intensity of light beam after absorption light. It is recommended not to touch the cuvette walls with hands.
ελ = molar extinction coefficient at wavelength λ Furthermore, in order to maintain the same conditions during the zeroing and the measuring
c = molar concentration of the substance phases, it is necessary to close the cuvette to prevent any contamination.
d = optical path through the substance

Therefore, the concentration "c" can be calculated from the absorbance of the substance as the FUNCTIONAL DESCRIPTION
other factors are known.
Photometric chemical analysis is based on the possibility to develop an absorbing compound INSTRUMENT DESCRIPTION
from a specific chemical reaction between sample and reagents. Given that the absorption of a
compound strictly depends on the wavelength of the incident light beam, a narrow spectral
bandwidth should be selected as well as a proper central wavelength to optimize measurements. 1) GLP/ key
The optical system of Hanna's HI 96715 colorimeter is based on a Light Emitting Diode (LED) 2) CAL CHECK key
combined with a narrow-band interference filter to guarantee both high performance and reliable 3) ZERO/CFM key
results. 4) READ/ /TIMER key
5) ON/OFF key
6) Liquid Crystal Display (LCD)
7) Cuvette alignment indicator
8) Cuvette holder
HI 96715 block diagram (optical layout)

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KEYPAD DESCRIPTION
• ON/OFF: to turn the meter on and off. ERRORS AND WARNINGS
• ZERO/CFM: this is a bi-functional key. Just press to zero the meter prior to measurement,
or to confirm edited values. In or to confirm factory calibration restore. The instrument shows clear messages when erroneous condition appears. Messages are also displayed
when the obtained values are outside expected range. The beeper is playing a beep on errors.
• READ/ /TIMER: this is a multi-functional key. In measurement mode, press to make a
measurement, or press and hold for three seconds to start a pre-programmed countdown prior
to measurement. In GLP mode press to view the next screen. a) on zero reading
• CAL CHECK: this is a bi-functional key. Just press to perform the validation of the meter, or
press and hold for three seconds to enter calibration mode. Light High: There is too much light to perform a measurement.
• GLP/ : this is a bi-functional key. Just press to enter GLP mode. In calibration mode press Please check the preparation of the zero cuvette.
to edit the date and time.

OPERATING MODES
• Measurement mode: default operation mode, enables both validation and measurement. Light Low: There is not enough light to perform a measurement.
• Calibration mode: may be entered by keeping CAL CHECK pressed for three seconds (the Please check the preparation of the zero cuvette.
“CAL” tag appears), it enables calibration of the instrument.
• GLP mode may be entered by pressing GLP/ (“GLP” appears), it enables consulting of
user calibration date or restore factory calibration.
No Light:The instrument cannot adjust the light level. Please
DISPLAY ELEMENTS DESCRIPTION check that the sample does not contain any debris.
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1 Inverted cuvettes: The sample and the zero cuvette are inverted.

1) The measuring scheme (lamp, cuvette, detector), appears during different phases of zero or
reading measurement
2) Error messages and warnings
3) The battery icon shows the charging level of the battery
4) The hourglass appears when an internal checking is in progress Zero: A zero reading was not taken. Follow the instructions of
5) Status messages the measurement procedure for zeroing the meter.
6) The chronometer appears when the reaction timer is running
7) The month, day and date icons appear when a date is displayed
8) Four digit main display
9) Measuring units
10) Four digit secondary display
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 Under range: A blinking “0.00” indicates that the sample Battery low: The battery must be replaced soon.
absorbs less light than the zero reference. Check the procedure
and make sure you use the same cuvette for reference (zero) and
measurement.

Over Range: A flashing value of the maximum concentration Dead battery: This indicates that the battery is dead and must
indicates an over range condition. The concentration of the be replaced. Once this indication is displayed, the meter will
sample is beyond the programmed range: dilute the sample and lock up. Change the battery and restart the meter.
re-run the test.

c) during calibration procedure GENERAL TIPS FOR AN ACCURATE MEASUREMENT

Standard Low: The standard reading is less than expected.
The instructions listed below should be carefully followed during testing to ensure best accuracy.

• Color or suspended matter in large amounts may cause

interference, therefore these should be removed by
treatment with active carbon and by prior filtration.
Standard High: The standard reading is higher than expected.
• For a correct filling of the cuvette: the liquid in the
cuvette forms a concavity on the top; the bottom of this
concavity must be at the same level of the 10 mL mark.

d) other errors and warnings • Proper use of dropper:

(a) to get good reproducible results, tap the dropper on the table for several times and wipe
Cap error: Appears when external light enters in the analysis the outside of the dropper with a cloth.
cell. Assure that the cuvette cap is present. (b) always keep the dropper bottle in a vertical position while dosing the reagent.

Cooling lamp: The instrument waits for the lamp to cool down.

(a) (b)

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 • It is important that the sample does not contain any debris.
This would corrupt the reading. STARTUP
Prepare the instrument for measurement as follows:
• In order to avoid reagent leaking and to obtain more • Unpack the instrument by removing the dust protection sleeve from the instrument cuvette
accurate measurements, it is recommended to close the holder.
cuvette first with the supplied HPDE plastic stopper and • Place the battery in the instrument as described in the “BATTERY REPLACEMENT” chapter.
then with the black cap.
• Place the instrument on a flat table.
• Do not place the instrument under direct sun light.
• Each time the cuvette is used, the cap must be tightened
to the same degree. MEASUREMENT PROCEDURE
• Whenever the cuvette is placed into the measurement To compensate the meter for the sample turbidity or color, the measurement takes place in two
cell, it must be dry outside, and completely free of phases. First, the meter is zeroed using the unreacted sample. After the reagents are added the
fingerprints, oil or dirt. Wipe it thoroughly with HI 731318 reacted sample is measured.
or a lint-free cloth prior to insertion.

• Turn the meter on by pressing ON/OFF

ON/OFF.
• Shaking the cuvette can generate bubbles in the sample, causing higher readings. To obtain
accurate measurements, remove such bubbles by swirling or by gently tapping the cuvette. The display briefly shows all tags on.

• Do not let the reacted sample stand too long after reagent is added, or accuracy will be lost.
• When the beeper sounds briefly and the
• It is possible to take multiple readings in a row, but it is recommended to take a new zero LCD displays dashes, the meter is ready.
reading for each sample and to use the same cuvette for zeroing and measurement.
The blinking “ZERO” indicates that the
instrument needs to be zeroed first.
• After the reading it is important to discard immediately the sample, otherwise the glass
might become permanently stained.

• All the reaction times reported in this manual are referred to 20°C (68°F). As a general rule • Fill the cuvette with 10 mL of unreacted
of thumb, they should be doubled at 10°C (50°F) and halved at 30°C (86°F). sample, up to the mark, and replace the
cap.
• In order to maximize accuracy, prior to a measurement follow the validation procedure to
be sure that the instrument is properly calibrated. If necessary, calibrate the instrument.

• Place the cuvette into the cuvette holder

and ensure that the notch on the cap is
positioned securely into the groove.

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• Press ZERO/CFM and the lamp, cuvette Alternatively, wait for 3 minutes and 30
and detector icons will appear on the seconds and just press READ/ /TIMER
/TIMER.
display, depending on the measurement In both cases, the lamp, cuvette and
phase. detector icons will appear on the display,
depending on the measurement phase.

• After a few seconds, the display will show

“-0.0-”. The meter is now zeroed and
ready for measurement. • At the end of measurement, the instrument
directly displays concentration in mg/L of
ammonia nitrogen (NH3-N) on the LCD.

• Remove the cuvette, open it and add 4 • To convert the reading to mg/L of
drops of HI 93715A-0 First Reagent (6 ammonia (NH3), multiply by a factor of
drops in case of seawater analysis). Swirl 1.216.
the cuvette gently to mix. INTERFERENCES
• Organic compounds like: chloramines, various aliphatic and aromatic amines, glycine,
(positive error).
To eliminate these interferences distillation is required.
• Add 4 drops of HI 93715B-0 Second
Reagent to the cuvette (10 drops in case • Organic compounds like: aldehydes, alcohols (e.g. ethanol) or acetone (negative error).
of seawater analysis). Replace the cap To eliminate these interferences distillation is required.
and swirl the cuvette to mix. • Sulfide (S2-): may cause turbidity.
• Hardness above 1 g/L as Calcium Carbonate (CaCO3).

VALIDATION PROCEDURE
• Replace the cuvette into the cuvette holder
and ensure that the notch on the cap is Use the validation procedure to ensure that the instrument is properly calibrated.
positioned securely into the groove. Warning: Do not validate the instrument with any standard solutions other than the HANNA CAL
CHECKTM Standards, otherwise erroneous results will be obtained. For accurate validation, please
perform test at room temperature: 18 to 25°C (64.5 to 77.0°F)

• Press and hold READ/ /TIMER for three • Turn the meter on by pressing ON/OFF.
seconds. The display will show the • When the beeper sounds briefly and the
countdown prior to measurement. The LCD displays dashes, the meter is ready.
beeper is playing a beep at the end of
countdown period.

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• Place the CAL CHECKTM Standard HI 96715-11 CALIBRATION PROCEDURE
Cuvette A into the cuvette holder and ensure that
the notch on the cap is positioned securely Note: It is possible to interrupt calibration procedure at any time by pressing CAL CHECK or
into the groove. ON/OFF keys.
Warning: Do not calibrate the instrument with standard solutions other than the HANNA CAL
CHECKTM Standards, otherwise erroneous results will be obtained. For accurate calibration, please
• Press ZERO/CFM and the lamp, cuvette and perform test at room temperature: 18 to 25°C (64.5 to 77.0°F)
detector icons will appear on the display,
depending on the measurement phase. • Turn the meter on by pressing ON/OFF.

• After a few seconds, the display will show

• When the beeper sounds briefly and the
“-0.0-”. The meter is now zeroed and ready
LCD displays dashes, the meter is ready.
for validation.

• Remove the cuvette.

• Press and hold CAL CHECK for three
• Place the CAL CHECKTM Standard HI 96715-11 seconds to enter calibration mode. The
Cuvette B into the cuvette holder and ensure display will show “CAL” during
that the notch on the cap is positioned securely calibration procedure. The blinking
into the groove. “ZERO” asks for instrument zeroing.

• Press CAL CHECKTM and the lamp, cuvette

and detector icons together with “CAL CHECK”
will appear on the display, depending on the • Place the CAL CHECK TM Standard
measurement phase. HI 96715-11 Cuvette A into the cuvette
holder and ensure that the notch on the
cap is positioned securely into the groove.
• At the end of the measurement the display
will show the validation standard value.

• Press ZERO/CFM and the lamp, cuvette

and detector icons will appear on the
The reading should be within specifications as reported in the CAL CHECKTM Standard
display, depending on the measurement
Certificate. If the value is found out of the specifications, please check that the cuvettes are free phase.
of fingerprints, oil or dirt and repeat validation. If results are still found out of specifications,
then recalibrate the instrument.

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• After a few seconds the display will show DATE INPUT
“-0.0-”. The meter is now zeroed and ready
• Press GLP/ to edit the desired year
for calibration. The blinking “READ” asks for
(2000-2099). If the key is kept pressed,
reading calibration standard.
the year number is automatically
increased.
• Remove the cuvette.
• When the correct year has been set, press or
ZERO/CFM or READ/ /TIMER to
• Place the CAL CHECKTM Standard HI 96715-11 confirm. Now the display will show the
Cuvette B into the cuvette holder and ensure month blinking.
that the notch on the cap is positioned securely
into the groove.
• Press GLP/ to edit the desired month
(01-12). If the key is kept pressed, the
month number is automatically increased.

• Press READ/ /TIMER and the lamp, cuvette

and detector icons will appear on the display, or
• When the correct month has been set,
depending on the measurement phase. press ZERO/CFM or READ/ /TIMER
to confirm. Now the display will show
the day blinking.

• After measurement the instrument will show

for three seconds the Cal Check Standard value. • Press GLP/ to edit the desired day
Note: If the display shows “STD HIGH”, the (01-31). If the key is kept pressed, the
standard value was too high. If the display day number is automatically increased.
shows “STD LOW”, the standard value was Note: It is possible to change the editing
too low. Verify that both CAL CHECK TM from day to year and to month by pressing
Standards HI 96715-11 Cuvettes, A and B are READ/ /TIMER.
free from fingerprints or dirt and that they are
inserted correctly. • Press ZERO/CFM to save the calibration
date.
Then the date of the last calibration (e.g.:
”01.08.2005”) appears on the display, or
“01.01.2005” if the factory calibration was
selected before. In both cases the year number • The instrument displays “Stor” for one
is blinking, ready for date input. second and the calibration is saved.

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• The instrument will return automatically • Press READ/ /TIMER to enter in the
to the measurement mode by displaying factory calibration restore screen. The
dashes on the LCD. instrument asks for confirmation of user
calibration delete.

• Press ZERO/CFM to restore the factory

GLP calibration or press GLP/ again to
abort factory calibration restore.
In the GLP mode, the last user calibration date can be consulted and the factory calibration can
be restored. • The instrument briefly notifies “done”
when restores factory calibration and
returns to measurement mode.
LAST CALIBRATION DATE
To display the calibration date:
• Press GLP/ to enter GLP mode. The
calibration month and day will appear on
the main display and the year on the
secondary display.
BATTERY MANAGEMENT

To save battery, the instrument shuts down after 10 minutes of non-use in measurement mode
• If no calibration was performed, the and after 1 hour of non-use in calibration mode.
factory calibration message, “F.CAL” will If a valid measurement was displayed before
appear on the main display and the auto shut off, the value is displayed when
instrument returns to measurement mode the instrument is switched on. The blinking
after three seconds. “ZERO” means that a new zero has to be
performed.
One fresh battery lasts for around 750 measurements, depending on the light level.
FACTORY CALIBRATION RESTORE The remaining battery capacity is evaluated at the instrument startup and after each measurement.
The instrument displays a battery indicator with three levels as follows:
It is possible to delete the calibration and
restore factory calibration. • 3 lines for 100 % capacity
• Press GLP/ to enter GLP mode. • 2 lines for 66 % capacity
• 1 line for 33 % capacity
• Empty battery icon blinking if the capacity is under 10 %.
If the battery is empty and accurate measurements can’t be taken anymore, the instrument
dead batt
shows “dead batt” and turns off.
To restart the instrument, the battery must be replaced with a fresh one.
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 BATTERY REPLACEMENT WARRANTY

To replace the instrument’s battery, follow the steps: HI 96715 is warranted for two years against defects in workmanship and materials when used
• Turn the instrument off by pressing ON/OFF. for its intended purpose and maintained according to the instructions.
This warranty is limited to repair or replacement free of charge.
• Turn the instrument upside down and remove the battery cover by turning it counterclockwise.
Damages due to accident, misuse, tampering or lack of prescribed maintenance are not covered.
If service is required, contact your dealer. If under warranty, report the model number, date of
purchase, serial number and the nature of the failure. If the repair is not covered by the warranty,
you will be notified of the charges incurred.
If the instrument is to be returned to Hanna Instruments, first obtain a Returned Goods Authorization
Number from the Customer Service Department and then send it with shipment costs prepaid.
When shipping any instrument, make sure it is properly packaged for complete protection.
To validate your warranty, fill out and return the enclosed warranty card within 14 days from the
date of purchase.
• Extract the battery from its location and replace it with a fresh one.
• Insert back the battery cover and turn it clockwise to close.

ACCESORIES

REAGENT SET
HI 93715-01 Reagents for 100 tests
HI 93715-03 Reagents for 300 tests

OTHER ACCESORIES
HI 96715-11 CAL CHECKTM Standard Cuvettes (1 set) Recommendations for Users
HI 721310 9V battery (10 pcs.) Before using these products, make sure that they are entirely suitable for your specific application and for the environment in
HI 731318 Tissue for wiping cuvettes (4 pcs.) which they are used.
HI 731331 Glass cuvettes (4 pcs.) Operation of these instruments may cause unacceptable interferences to other electronic equipments, this requiring the operator
to take all necessary steps to correct interferences.
HI 731335 Caps for cuvettes
Any variation introduced by the user to the supplied equipment may degrade the instruments' EMC performance.
HI 93703-50 Cuvettes cleaning solution (230 mL) To avoid damages or burns, do not put the instrument in microwave oven. For yours and the instrument safety do not use or
store the instrument in hazardous environments.

Hanna Instruments reserves the right to modify the design, construction and appearance of its
products without advance notice.

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Hanna Instruments Inc.
Highland Industrial Park
584 Park East Drive
Woonsocket, RI 02895 USA

Technical Support for Customers

Tel. (800) 426 6287
Fax (401) 765 7575
E-mail tech@hannainst.com
www.hannainst.com

Local Sales and Customer Service Office

Printed in EUROPE
(ROMANIA) MAN96715 07/10

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