Automated ELISA System: Operator's Manual

DSX™ Automated ELISA System
Operator’s Manual
IMPORTANT
Please read carefully before using the DSX
Part No. 91000060 Version 07-2000
This manual is published by DYNEX TECHNOLOGIES INC.
Questions or comments regarding the content of this manual can be
directed to the address below or to your supplier.
DYNEX TECHNOLOGIES INC.
14340 Sullyfield Circle
Chantilly, VA 20151-1683 USA
®
Microtiter is a registered trademark of DYNEX TECHNOLOGIES INC.
 2000 This document is the copyright of DYNEX TECHNOLOGIES and

must not be copied or reproduced in any form without prior consent.
DYNEX reserves the right to make technical improvements to this
equipment and documentation without prior notice as part of a continuous
program of product development. This manual supersedes all previous
editions.
Table of Contents
Table of Contents
Table of Contents ......................................................................................................... i
About this Manual........................................................................................................iii
Chapter 1 Overview.................................................................................................... 1
Introduction .......................................................................................................... 1
Samples.......................................................................................................... 1
Reagents ........................................................................................................ 2
Pipetting.......................................................................................................... 2
Dilutions.......................................................................................................... 2
Incubation ....................................................................................................... 2
Washing ......................................................................................................... 2
Detection ........................................................................................................ 2
Features............................................................................................................... 3
Chapter 2 Description................................................................................................. 5
Hardware Components........................................................................................ 5
Indicator Light ................................................................................................. 5
System Cover ................................................................................................. 6
Workspace ..................................................................................................... 7
Ambient Drawer.............................................................................................. 9
Plate Holders ................................................................................................ 10
Pipette Module.............................................................................................. 11
Sample Mixing During Dilution ..................................................................... 12
Pre-Dilution................................................................................................... 13
Incubator Modules ........................................................................................ 14
Wash Module ............................................................................................... 15
Absorbance Module...................................................................................... 22
Software............................................................................................................. 26
DSX Configuration........................................................................................ 27
Consumables Management ......................................................................... 28
Assay Definition............................................................................................ 29
Worklist Creation .......................................................................................... 31
Worklist Execution........................................................................................ 31
Data Analysis................................................................................................ 31
Results Reporting ......................................................................................... 31
Data Backup ................................................................................................. 31
Required But Not Provided........................................................................... 32
Computer...................................................................................................... 32
Specifications..................................................................................................... 33
Warning Labels.................................................................................................. 35
Chapter 3 Installation................................................................................................ 37
Unpacking.......................................................................................................... 37
Materials Provided........................................................................................ 37
Positioning the Instrument ................................................................................. 38
Connecting the Computer System..................................................................... 39
Connecting to a LIMS ........................................................................................ 40
Checksum .................................................................................................... 41
Loading Revelation™ Software ......................................................................... 42
Creating a Shortcut Icon............................................................................... 43
DSX™ System Operator’s Manual i

Table of Contents
Connecting the DSX Power Cord ...................................................................... 44

Starting the System ........................................................................................... 45
Self Tests .......................................................................................................... 46
Chapter 4 Preparing the System for Use ................................................................. 47
Configuring the Reader ..................................................................................... 47
Editing Washer Plate Settings........................................................................... 49
Selecting the Results Font................................................................................. 51
Setting Options .................................................................................................. 52
Changing the Password .................................................................................... 53
Specifying Consumables ................................................................................... 54
Filling the Wash Buffer Containers.................................................................... 55
Connecting the Waste Container ...................................................................... 56
Chapter 5 Defining an Assay..................................................................................... 57
Creating a New Assay ....................................................................................... 57
Modifying an Assay............................................................................................ 58
Chapter 6 Creating a Worklist .................................................................................. 59
Creating a New Worklist.................................................................................... 59
Modifying a Worklist .......................................................................................... 60
Chapter 7 Running a Worklist .................................................................................. 61
Preparation ........................................................................................................ 61
Starting the Run................................................................................................. 63
Monitoring Run Status ....................................................................................... 64
Chapter 8 Service and Maintenance ........................................................................ 65
Routine Maintenance Procedures ..................................................................... 65
Cleaning and Decontamination ......................................................................... 67
Removing a Module........................................................................................... 68
Replacing the Absorbance Module Lamp ......................................................... 69
Replacing an Absorbance Module Filter............................................................ 71
Replacing the Tubing......................................................................................... 73
Cleaning the Wash Head .................................................................................. 75
Chapter 9 Service..................................................................................................... 77
Returning a Module for Service ......................................................................... 77
Limited Warranty ............................................................................................... 79
Warranty and Special Provisions ................................................................. 79
Sales Offices ..................................................................................................... 80
Index.......................................................................................................................... 81
ii DSX™ System Operator’s Manual

Introduction
About this Manual

This manual has been written for laboratory technicians and provides
detailed instructions for using the DSX™ Automated ELISA System.
This manual gives you the information needed to:
• Install the DSX™
• Set up the DSX™ to suit your specific application requirements
• Understand the DSX™ menus
• Run assays using the DSX™
• Create or modify assays
• Perform required preventive maintenance
• Service the DSX™
• Review safety precautions
DSX™ System Operator’s Manual iii

Introduction
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iv DSX™ System Operator’s Manual

Introduction
Chapter 1 Overview
Introduction
The DSX™ Automated ELISA System (Figure 1) is a computer-controlled
microplate processing system that fully automates ELISA assays. The DSX
System automates the sample distribution, incubation, reagent addition,
washing and detection phases of microplate assays. It is intended for use in
clinical, research and industrial laboratories.
The DSX is an automated system that is useful for medium throughput,
multiple assay applications. The system is designed to process specimens
in a continuous flow from sample to result. Operator attendance is normally
not required once a run has started.
Samples
A run can contain up to 96 samples. Serum and/or plasma specimens are
generally used for clinical testing, although other fluids such as urine or
spinal fluid can be run. Specimens containing large particulate matter, such
as stool, tissue homogenates or culture media, can also be used. It is
recommended that these specimens are processed through a mesh filter
prior to loading onto the system to assure sample homogeneity and
pipetting precision.
A wide variety of standards or control samples can also be run. Standards
or controls are loaded onto a separate rack that contains up to 33
standard/control tubes. The tubes are available from your supplier.
Figure 1. The DSX™ Automated ELISA System
DSX™ System Operator’s Manual 1

Introduction
Reagents
The DSX System utilizes a single reagent rack that contains up to 24
bottles. The required reagent bottles are available from your supplier.
Pipetting
Pipetting of samples, standards/controls and reagents is performed using
custom-designed disposable pipette tips to assure pipetting precision and
eliminate possible cross-contamination. Reagent tips pipette from 25 µL to
1 mL of reagents, and sample tips pipette from 5 µL to 300 µL of samples
or standards/controls.
Dilutions
The DSX System will perform single-stage or multi-stage dilutions of
sample. Dilutions ranging from 1:2 to 1:40,000 can be performed, using
deep-well dilution plates on the system for dilutions above 1:60.
Incubation
Up to four temperature-controlled plate incubators may be present. Each
incubator can be set at a temperature ranging from ambient plus 7 °C to
50 °C, and each plate can be shaken during incubation. The particular
incubator modules that are used during a worklist, the temperature of each
incubator, and the shake parameters are automatically set by the system
when the worklist is created.
Washing
Eight wells in one column of a 96-well plate can be washed simultaneously.
Washing protocols can be defined so that all of the columns are washed in
the same manner, or different wash cycles can be applied to specified
columns on a plate.
A wide variety of user-defined wash protocols can be programmed on the
system. In addition, different plate types can be accommodated.
Detection
During operation, each microplate is automatically transferred to the
absorbance module at the appropriate time. The optical densities of the
wells specified during assay definition are read, the various calculations (for
example, blanking, QC of raw data, threshold or curve fitting) are applied,
and the calculated results for the microplate are reported.
2 DSX™ System Operator’s Manual

Features
Features
The DSX™ Automated ELISA System has a number of performance and
convenience features. These are summarized below:
• Different assays can be run on the same plate

• ESP™ (Electronic Signature Pipetting) for liquid level and clot
detection
• Endpoint data analysis to perform qualitative and quantitative data
reduction
• Less than 10 second reading time (using single wavelength)
• On-board self diagnostics
• Selection of up to six filters
• Single, dual and multiple wavelength reading modes
• Easily removable incubator, wash, and absorbance modules for
servicing
• Small footprint
• A variety of wash protocols can be programmed
• A variety of plate types can be programmed
• Liquid level sensing on Waste Container and Wash Buffer
Containers
• Quick dispense
• Aspirating/pipetting speed can be changed for viscous liquids

Features

Hardware Components
Chapter 2 Description
Hardware Components
Locations of the principal hardware components of the DSX™ Automated
ELISA System are shown in Figure 2.
Indicator Light
The system contains an indicator light (Figure 3) that is illuminated
whenever the system power is ON.
! CAUTION: Power is on to the system and to the

incubator heaters whenever the indicator light is
illuminated. A thermal hazard is present.
Wash Buffer/ Pipette Work System Absorbance Wash Incubator

Dispense Containers Module Area Cover Module Module Modules
Ambient
Drawer
Liquid Waste
Container
Waste Tip
Container
Figure 2. Location of Principal Hardware Components

Hardware Components
System Cover
The system cover encloses the workspace and pipette module. The cover
must be closed during operation to prevent the pipette module from
accidentally contacting an operator or bystander. An electrical interlock
prevents operation of the pipette module when the system cover is open.
! CAUTION: The electrical interlock for the system

cover prevents accidental contact with the pipette
module and/or robotic arm. Never disable the
interlock unless instructed to do so by DYNEX
personnel.
To open the cover, lift the handle until the cover is in the upright position
(Figure 3). The cover will remain in this position until it is closed.
To close the cover, push down on the handle until the cover is fully closed
and locked. The system cover rests on the cover stop when it is fully
closed.
! CAUTION: Pinching hazard. Be sure that your hands

and fingers are clear of the cover stop when closing
the system cover.
Handle System Cover
Indicator
Light
Cover
Stop
Figure 3. System Cover

Hardware Components
Workspace
Samples, reagents, standards and controls, and consumables are loaded
onto the workspace. Their locations are shown in Figure 4.
Sample tubes are contained in seven sample racks. Each sample rack
contains 14 tubes and, although up to 98 samples can be contained on the
system at one time, 96 sample tubes are used in routine operation. The
seven sample racks are contained in a sample caddy. Since different
sample tubes can be used on the system, the user must specify the sample
tube dimensions before the tubes can be used.
Standards or controls are contained in the control rack, which contains up
to 33 standard/control tubes. A specific 1.5-mL tube is required for
standards or controls. The tubes can be obtained from your supplier.
Sample tips used for pipetting samples and standards/controls are
contained in four sample tip racks. Each rack contains 108 sample tips,
and a total of 432 sample tips can be loaded on the system. A specific
sample tip is required and can be obtained from your supplier.
Reagent tips used for pipetting reagents are contained in a reagent tip
rack that contains 41 reagent tips. A specific reagent tip is required and can
be obtained from your supplier.
Sample Dilution Reagent Reagent Tip Waste

Racks Plate Rack Tip Rack Chute
Sample
Tip Racks
Reagent
Tip Rack
Dilution
Plate
Control
Rack
Figure 4. The Workspace

Hardware Components
Reagent bottles are contained on the reagent rack, which contains up to

24 reagent bottles. A specific 25-mL reagent bottle is required and can be
obtained from your supplier.
Two deep-well dilution plates can also be loaded. The dilution plates are
used for two-stage (external) dilutions ranging from 22:1 to 1:36,100.
After a sample tip or reagent tip has been used, the pipette module moves
over the tip waste chute and releases the tip. The tip waste chute directs
the used sample tip or reagent tip into the waste container.

Hardware Components
Ambient Drawer
The ambient drawer is used to store microplates when room temperature
incubation in the dark is required. The ambient drawer will extend into the
work area during pipetting.
When setting up a worklist, the plate carrier is extended from the ambient
drawer (Figure 5), plate holders (see the following page) are placed in
each of the ambient drawer positions, and the required microplates are
placed onto the plate holders.
! The microplate positions are numbered from 1 to 4.

Refer to page 59 for a summary of the procedure to
prepare a worklist.
Position 1 Position 2 Position 3 Position 4
Figure 5. The Ambient Drawer

Hardware Components
Plate Holders
Plate holders (Figure 6) allow the pipette module to transfer a microplate
between modules.
Each plate holder has a pickup feature which the pipette module uses to
transfer a microplate.
! Whenever this manual refers to a microplate, it is

implied that the microplate is seated in a plate holder .
Gripping Positioning
Slot Pins
Figure 6. Plate Holder

Hardware Components
Pipette Module
The pipette module is used to transfer microplates, to pipette samples,
controls and standards, dispense reagents, and to perform dilutions. The
pipette module travels in the x-, y- and z- directions to access the samples,
controls, reagents, microplates and consumables on the workspace. The
pipette module is shown in Figure 7.
The pipette module has the following functions:
Function Purpose
Microplate The microplate handler transfers a microplate

Handling between the ambient drawer, an incubator module,
the wash module and the absorbance module by
gripping the microplate holder, moving it to its new
location, and releasing it.
Pipetting The pipettor pipettes samples or standards/controls
(automatic using disposable sample pipette tips. Dispenses
liquid level reagents using disposable reagent pipette tips.
sensor) Each pipette tip is automatically discarded into the
waste bin after use. A new pipette tip is obtained
from a sample tip rack or the reagent tip rack when
it is needed.
Tip Ejection Verifies that a used tip was ejected before obtaining
Detection a new tip.
Pipette Module
Microplate
Holder Clamp
and Tip Ejector
Pipettor
Figure 7. The Pipette Module

Hardware Components
Separate pipetting profiles ranging from 1 to 5 can be specified for any

fluid except wash buffer. The pipetting profile specifies the rate at which
fluids are aspirated or dispensed from the pipette tip.
The pipetting system of the DSX™ Automated ELISA System includes
ESP™ (Electronic Signature Pipetting) software for automatic detection of
clots, foam, or bubbles when pipetting samples. The pipeting signature
observed when pipetting each sample is compared to nominal pipetting
signatures of particular sample types (for example, serum or plasma)
obtained during system configuration. If the pipetting signature does not fall
within the normal range of pipetting profiles for that sample type, the system
records an error.
! CAUTION: Changing the pipette profile can affect

accuracy and precision of an assay.
! The default pipetting profile is 4. The pipetting profile

used for samples is specified during definition of
worklist runtime parameters. Refer to page 61 for a
summary. The pipetting profiles used for standards
and controls are specified during definition of assay
operations. Refer to page 29 for a summary.
! The pipetting signature is specified during definition of

worklist runtime parameters. Refer to page 61 for a
summary
Sample Mixing During Dilution

The user can program up to 9 dispense/aspirate mix cycles during dilution
steps to achieve optimal mixing of samples during dilution.

Hardware Components
Pre-Dilution
The DSX™ Automated ELISA System can perform a pre-dilution of
samples before they are assayed. Pre-dilution can be performed in a single
stage in standard microplates or in two stages using deep-well microplates.
The system can be programmed to add diluent to a plate before or after the
sample is added.
The dilution modes are described below:
Dilution Mode Description
Microplate Ratios of sample to diluent range from 11:1 to

Dilution 1:59 (e.g. 5 µL sample combined with 295 µL of
diluent to yield a 1:59 dilution.
Deep-well Plate Ratios of sample to diluent range from 2:1 to
Dilution 1:190 (e.g. 5 µL sample combined with 950 µL
of diluent to yield a 1:190 dilution.
plus Microplate 1:11,210 (e.g. 5 µL sample combined with
Dilution 950 µL of diluent in a deep-well to form a new
sample from which 5 µL is combined with
295 µL of diluent in a microplate to yield a
1:11,210 dilution.
plus Deep-well 1:36,100 (e.g. 5 µL sample combined with
Plate Dilution 950 µL of diluent in a deep-well to form a new
sample from which 5 µL is combined with
950 µL of diluent in another deep-well to yield a
1:36,100 dilution.

Hardware Components
Incubator Modules
Microplates are incubated and shaken in the incubator modules. A
maximum of four incubator modules are present, so that different
microplates can be incubated at different temperatures with or without
shaking. Incubation temperature and shake duration are specified during
definition of an assay.
! Note: Refer to page 29 for a summary of the

procedures to define an assay. A microplate can also
be incubated at ambient temperature without shaking
by allowing it to remain in the ambient drawer for a
specified period of time.
The particular incubator modules that are used during a worklist and the
temperature and shaking of each incubator are automatically set by the
system when the worklist is created. Processing of the worklist will not
commence until the temperature of each required incubator module is at the
correct value.
! Note: The incubator modules are labelled as 1, 2, 3

and 4 and are designated as such in the Revelation™
software. The incubator modules will control
microplate temperature at any specified temperature
ranging from ambient plus 7 °C to 50 °C.
Incubator
Modules
4
1 Ambient
Drawer
2
Figure 8. Incubator Modules

Hardware Components
Wash Module
The well contents of a microplate are washed in the wash module
(Figure 9). The wash module is designed to wash all 8 wells in one column
of an 8 x 12 microplate simultaneously. The washing protocol can be
defined to wash partially filled plates containing complete columns.
Different user-defined wash protocols can be contained on the system. In
addition, the system can be configured with different plate types so that the
wash head positions for each plate type can be specified. The system can
accommodate flat-bottom, C-bottom, U-bottom and V-bottom types of
microplates.
! Note: Refer to page 29 for a summary of the wash

protocol alternatives.
Wash Head
The wash head contains two sets of wash pins. The shorter pins (the
dispense pins) dispense fluid and the longer pins (the aspirate pins)
aspirate fluid. The aspirate pins and the dispense pins are closely spaced
so that fluid can be aspirated from and dispensed into wells at the same
time.
The wash pins are fixed to the wash head. During operation, the wash head
assembly is lowered to insert the wash pins into the wells or raised to
remove the wash pins from the wells. Lowering the wash head assembly
allows the well contents to be aspirated or a bottom wash to be performed.
Raising the wash head assembly allows the wash head to be moved so
another column can be washed or so the wells can be filled.
Plate Wash Dispense/Aspirate

Drawer Head Pins
Figure 9. Wash Module

Hardware Components
Wash Head Positions

The vertical positions that the wash head can assume (Figure 10) are
described below. Each wash head position can be specified by the user to
within 0.1 mm.
! Note: Wash head positions for various plate types are

specified during consumables management. See
page 28 for additional information.
Wash Head Position Description
Dispense Height Defines the position at which fluid will be

dispensed. While it is often desirable to set
this height just above the top of the well in
order to form a positive meniscus, the user
should ensure that an overflow does not
occur.
Top of Well Positions the aspiration pins so they are
aligned with the top of the well. At this
position, the liquid meniscus is removed
from the top of the well.
Aspiration Height Positions the aspiration pins at the bottom
of the well so that the contents of the well
can be completely aspirated.
Sweep Height Raises the aspiration pins slightly above
the Aspiration Height (see above) so that
the aspiration pins can be moved back and
forth in the well while the fluid is being
aspirated without the danger of scratching
the bottom of the plate.
Bottom Wash Height Lowers the wash head to this height during
dispense so that the force of the
dispensed fluid can wash the bottom of the
wells.
Sweep Stroke Defines the horizontal motion used during
a sweep operation to fully aspirate well
contents.
! Note: Select No Sweep for sweep mode and disable

bottom washing whenever a C-bottom, U-bottom or
V-bottom plate is being used.

Hardware Components
Dispense
Aspirate
Microplate
Figure 10. Position of the Wash Pins
Wash Protocol Operations

A wash protocol consists of a series of Purge, Move, Soak, Aspirate,
Dispense and Fill operations. Purge, Move and Soak can be carried out in
any sequence. Aspirate, Dispense and Fill can only be carried out within a
Move operation, and there cannot be a Move within a Move.
Each of these operations is summarized below:
Operation Description
Purge Dispenses fluid from the dispense wash pins while

the wash head is positioned over the purge tray. A
purge is usually carried out at the beginning of a
wash protocol or at the end of the day to rinse the
dispense wash pins and remove air bubbles.
Move Performs Aspirate, Dispense, Fill and/or Soak
operations on specified strips of the Microplate.
Aspirate Removes the contents of a well by positioning the
wash pins at the aspiration height in the well and
aspirating the liquid from the wells.
A sweep may also be performed during Aspirate.
Dispense Dispenses a specified amount of fluid into the wells
after aspirating the contents of the wells. If a
bottom wash is specified, the wash head is then
lowered to the bottom wash position so that fluid
will be aspirated from the bottom of the wells while
fluid is being dispensed.
Soak The contents of the wells are allowed to equilibrate
for the specified number of seconds.
Fill The wells are filled with a specified amount of fluid.

Hardware Components
Wash Head Sweep Modes
Sweep modes specify whether the aspiration tip moves from side-to-side
during aspiration. Five sweep modes can be used:
• No sweep
• Always sweep
• Sweep on last cycle only
• Always Super Sweep
• Super sweep last cycle only
! Note: Select No Sweep for sweep mode and disable

bottom washing whenever a C-bottom, U-bottom or
V-bottom plate is being used.
Plate Drawer
The plate drawer holds the microplate in a known position so that the wash
pins are precisely positioned in the wells during various wash protocol
operations. The plate drawer allows linear shaking of the plate.
Wash Buffer Containers

Up to four different washing and/or dispensing reagents are contained on
the system in wash buffer containers. The wash buffer containers are
located at the front of the instrument (Figure 11).
Each container contains up to two liters of wash buffer. Dispensing of wash
buffer from a container is controlled by a submersible pump in the wash
container, a dispense valve above the wash bottle, and a dispense valve
located near the wash head. The specified wash buffer is dispensed into
wells whenever a Dispense or Fill operation is specified in the wash
protocol.
Each wash buffer container must contain at least 500 mL of wash buffer in
order to be used.
! Note: The particular wash buffer that is used and the

Dispense, Fill or Purge operations are defined during
assay definition. See page 57 for a summary of the
procedures for assay definition.
A quick connect fitting and a level sensor/pump connector allow easy

removal of a wash buffer container from the system (Figure 12). Disconnect
the wash line by pressing on the metal tab of the quick connect fitting and
gently pulling up on the wash line to remove it. Disconnect the level sensor
and pump connector by pulling it out of the connector socket.
Fill (or empty) a wash buffer container using the filler cap at the rear of the
container.

Hardware Components
Dispense Dispense Dispense Dispense

Valve A Valve B Valve C Valve D
Wash Buffer Wash Buffer Wash Buffer Wash Buffer

Container A Container B Container C Container D
Figure 11. Wash Buffer Containers and Wash Buffer Dispense Valves
Level Sensor and

Pump Connector
Filler Cap
Quick Connect
Fitting
Figure 12. Wash Buffer Container Details

Hardware Components
Waste Containers
Fluid that is removed during purging and washing is collected in the liquid
waste container. Used sample and reagent pipette tips are disposed into
the tip waste container. Both waste containers are located at the front of
the instrument (Figure 13).
Tip Waste Liquid Waste

Container Container
Figure 13. Waste Containers
The liquid waste container (Figure 14) holds up to eight liters of waste. A
level sensor alerts the operator when the liquid waste container is full.
Two quick connect fittings connect the liquid waste and vacuum lines to
the liquid waste container. Disconnect each fitting by pressing on the metal
tab of the quick connect fitting and gently pulling on the line to remove it.
Disconnect the level sensor connector by pulling it out of the connector
socket.
Empty the liquid waste container by removing the waste cap at the front of
the container.
! Note: Be sure that the waste cap is securely

tightened. Otherwise, a vacuum leak will cause the
software to create a vacuum error condition.
! Note: 10% (v/v) solution of household bleach in water

is recommended as a disinfectant.

Hardware Components
Level Sensor Manifold Vacuum Waste

Connector Connector Connector Cap
Figure 14. Liquid Waste Container

Hardware Components
Absorbance Module
The absorbance module measures the optical density (OD) of the final
reaction mixture in the microplate wells. The wavelength mode that is used
and the wavelength(s) at which the optical density is measured are
specified during assay definition.
! Note: Procedures for specifying the wavelength mode

and the wavelength(s) at which the optical density is
measured are defined during assay definition. See
page 57 for a summary of the procedures for assay
definition.
! Note: The current version of Revelation™ software

supports endpoint reactions.
During operation, each microplate is automatically transferred to the

absorbance module at the appropriate time. The optical densities of the
wells specified during assay definition are read, the various calculations (for
example, Blanking, QC Raw Data, Threshold or Curve Fitting) are applied,
and the calculated results for the microplate are reported.
! Note: Procedures for specifying the manner in which

assay results are calculated and reported are defined
during assay definition. See page 57 for a summary
of the procedures for assay definition.
The location of the absorbance module is shown in Figure 15.
Absorbance
Module
Ambient
Drawer
Figure 15. Absorbance Module

Hardware Components
Optical Path
The optical path through the Absorbance module is shown in Figure 16. A
tungsten halogen lamp projects a light beam through a heat absorbing filter
and a lens. The beam is focused by the lens and passes through a filter
(located on the filter wheel), which allows only light of the desired
wavelength range to pass. The beam is then separated into 13 channels,
one of which is used as a reference channel to monitor the light output of
the lamp. The other 12 beams are directed upwards through a row of 12
wells on the microplate, onto an array of silicon photodiodes. The silicon
photodiodes quantify the intensity of light transmitted through the reaction
solution. Absorbance of the solution is measured in terms of optical density
(OD) and the assay results are interpreted accordingly.
1 Lamp 6 Lenses
2 Heat Filter 7 Optic Stops
3 Lens 8 Microplate
4 Filter 9 Photodiodes
5 Optic Fibers 10 Reference Diode
Figure 16. Optical Path of the Absorbance module

Hardware Components
Single and Dual Wavelength Modes

The Reader is able to take readings in three different modes:
• Single--using one test wavelength
• Dual--using one reference wavelength and one test wavelength
• Multiple--using a combination of wavelengths
The Single wavelength mode is sufficient for most applications.
The Dual wavelength mode can be used if it is necessary to reduce errors
caused by dirt and scratches on the bottom of the wells.
The choice of test and reference wavelengths for the Dual Wavelength
mode depends on the particular enzyme/substrate system being tested.
However, the following rules should usually be followed:
1. The test wavelength (λt) should be at or near the maximum

absorbance of the reaction product.
2. The reference wavelength (λr) should lie outside the

absorbance band of the system but not far removed.
405 nm λt λr 690 nm
FILTER WAVELENGTH
Figure 17. Dual Wavelength Selection

Hardware Components
The Reader subtracts the absorbance at the reference wavelength (λr) from
the absorbance at the test wavelength (λt) to minimize the effect of
systematic errors.
If a test requires particular precision, you may specify test and reference
filters of the same wavelength. The Reader will average the ODs produced
using each filter, giving a more precise result.
Multiple Wavelength Mode

The Multiple wavelength mode reads samples at two different wavelengths
and is used to obtain results where the peak absorbance is outside the
optical range of the Reader.
The first reading (or the Primary mode) is at or near the peak wavelength. A
second reading (or the Secondary mode) of the sample is then obtained at
a wavelength that is within the absorbance region but not at the peak.
The Reader automatically uses the Primary mode reading when it
calculates results. If, however, the absorbance in the Primary mode
exceeds the detection limit of the Reader, the Secondary mode reading is
used.
If the Secondary mode reading is used, the peak absorbance is calculated
from the secondary mode reading using an algorithm that is selected by the
user during configuration of the system.
Blanking
The Reader lets you subtract a reference value from all the ODs. It
automatically uses air as a reference, but for certain applications other
reference levels may be more appropriate.
For example, you may want to eliminate the absorbance of a reagent
solution from the test result. The Reader can hold the OD of this reagent
solution in memory and subtract it from all subsequently read ODs.
Blanks may be single wells, or an average of wells.

Software
Software
Revelation™ software is used to control the DSX microplate processing
system. The software automates the sample distribution, incubation,
reagent addition, washing and detection phases of microplate assays. It
also provides the user interface for configuration of the instrument and
management of consumables (Figure 18).
The software includes an extensive menu of assay definition options that
allow you to customize the readings, calculations, QC checks and results
format for an assay.
Additional information about the software can be found in the DSX Online
Operator’s Manual, accessed by selecting the Help menu.
DSX Configuration
Consumables
Management
Assay Definition
Worklist Creation
Data Verification
Worklist Execution
Data Analysis
Results Reporting
Figure 18. Revelation™ Software Overview

Software
DSX Configuration
The DSX System can be configured for particular uses in your laboratory.
Refer to Chapter 4 (Preparing the System for Use) for instructions.
The configuration options are summarized below:
Option Description
Configure Reader Define COM port, filters, conversion limits,

maintenance scripts, and log file processing.
Select Fonts Select the font to be used for printed results
reports.
Set Options Create short-cut buttons.
Set default plate processing options, including
auto save and auto print.
Select the colors for different well types.
Set default directories.
Enter the laboratory name and address to be
printed on results reports.
Assign a System Change the default password in Revelation™
Password software.

Software
Consumables Management
A database of all consumables and fluids is maintained in the software.
Once a consumable or fluid is defined in the database, it can be selected
from a drop-down list during definition of an assay.
Consumables and fluids are defined using the Tools menu. The fluids and
consumables that are defined and the information that is entered for each
are summarized below:
Consumable Information Entered
Washer Fluids Define the fluids that are used for washing and
purging.
Sample Tubes Define sample tubes and their specific
dimensions.
Reagent/Diluent Define reagents and diluents, and specify
Fluids whether they are time-sensitive. If they are,
specify the maximum on-system use time.
Specify the pipette profile used when aspirating
and dispensing a reagent or diluent. The profile
should be set to 4 unless otherwise required.
Standard/ Define standards and controls, and specify

Control Fluids whether they are time-sensitive. If they are,
specify the maximum on-system use time.
Specify the pipette profile used when aspirating
and dispensing a standard or control.
Washer Plates Specify the height of the washer head for
various washing positions (for example, well top
height, dispense height and wash height) for
each type of microplate that is used.
Specify sweep modes that can be used during
washing and their parameters.
Specify whether bottom washing can be used
during washing.

Software
Assay Definition
The reagents, standards and controls that are used and the dilution, wash
and incubation procedures for an assay are specified during assay
definition. These options are defined using the Operations menu.
Arrangement of well types on the microplate, reading options, and
procedures to be used for calculations, QC checks and reporting when
running a test are also specified during assay definition. These options are
defined using the Data Reduction menu.
The assay definition options are summarized below:
Option Purpose
Operations Menu:
Pipette Samples/ Specify plate templates.
Standards/ Specify sample, standard or control volumes.
Controls Specify pipetting techniques and tip usage.
Specify dilutions.
Dispense Fluid Specify reagent volumes.
Wash Plate Specify plate types, wash methods and wash
buffer volumes.
Specify details for the Purge and Clean cycles.
Specify details for the Soak and Shake options.
Incubate Plate Specify incubation time, temperature and
shaking.
Data Reduction Menu:

Data Reduction Displays the Data Reduction dialog boxes in
Wizard the order they appear on the menu, starting
with Assay Title.
Assay Title Specify assay title, author, code and password.
Reader Control Specify assay wavelength(s), wavelength mode
and shake.
Template Specify the use (sample, control, blank, etc.) of
each well on the plate, including the number of
replicates, orientation, and fill direction.
Blank Mode Select a pre-defined blank mode or define a
custom blank mode.

Software
Option (Cont’d) Purpose
Data Reduction Menu:

QC Raw Data Define a quality control equation to be applied
to the raw data obtained when running the
assay.
Threshold Define threshold equations, positive/negative
Q.C. ranges, threshold Q.C. equations, and the
results output format.
Curve Fit Specify the parameters required to obtain
quantitative results with a user-defined curve fit.
Ratio Define the equation and output format required
to report results as a ratio.
Spreadsheet Perform arithmetic calculations on ODs from
different wells. The results of these calculations
are output as a matrix or table of data.
Report Options Select report formats, matrix type, and export
files.
Assay Options Specify area statistics, processing order, and
sample ID setup.

Software
Worklist Creation
A worklist specifies the samples that are to be run. A worklist can include
up to four microplates and multiple assays. More than one assay can be run
on a plate if the assays have the same incubation, washing and shaking
specifications.
! Note: An assay must be created before it can be

assigned to samples on a worklist.
! Note: The procedure for creating a worklist is

summarized on page 59.
Worklist Execution
Once a worklist has been created, the run can be started. The system
prompts the operator to load any microplates and consumables that are
required by the worklist.
! Note: The procedure for running a worklist is

summarized on page 61.
Data Analysis
The optical density results for each well on each of the microplates in the
worklist are analyzed according to the criteria that were specified during
assay definition.
Results Reporting
When the processing of a microplate is completed, the results of the run are
displayed on the screen.
Results files are automatically named xxx.dat, where xxx is the name you
selected for the plate. Results are stored in the Plate Data directory.
! Note: Select the Options command on the Tools

menu to view or change a directory in which results
files or other files are located.
Data Backup
Revelation™ software allows the user to save all assays and worklists on
floppy diskettes to avoid loss of data.

Software
Required But Not Provided
Computer
A personal computer with monitor, keyboard, mouse and printer is required
for operation of the DSX™ Automated ELISA System.
The computer system provides the means for you to enter information and
obtain results. The computer stores and retrieves assay profiles, executes
the defined worklist, and performs the various calculations needed for the
assay.
The computer system that is used for operation of the DSX™ Automated
ELISA System must meet the following minimum requirements:
• Pentium microprocessor running at 500 MHz or better.
• Hard disk with at least 100 MB of free space.
• Microsoft® Windows® NT operating system.
• VGA graphics card (Super VGA recommended). Monochrome,
CGA, EGA or calibrated monitors are not supported.
• The Display properties should be set to a desktop area of at least
600 x 800 pixels and a color palette of at least 256 colors.
• At least 64 megabytes (MB) of random-access memory (RAM).
(128 MB RAM is recommended).
• One unused RS232 serial port is required for connecting the
computer to the DSX™ Automated ELISA System.
• Mouse or other pointing device supported by Windows.
• Any printer that is supported by Windows® NT can be used.
• Compatible sound card

Specifications
Specifications
Dimensions and Weight

Depth <91 cm (35.8 in)
Width <106 cm (41.8)
Height <80 cm (31.5 in)
Weight <110 Kg (243 lbs)
Footprint 106 cm x 91 cm (41.8 in x 35.8 in)
Capacities
Samples 96
Reagents 24
Controls and/or Standards 33
Sample Pipetting Tips 432
Reagent Pipetting Tips 41
Wash Buffer Bottles 4 bottles, 2 liters each
Waste Container 8 liters
Operation
Ambient Drawer Module Ambient plus 5 °C
Incubation Temperatures
Incubator Module Ambient plus 7 °C to 50 °C

Incubation Temperatures
Assays
Blanking Air
Individual, paired or average wells
Whole plate or last plate
Row or column
Each well on the plate
Wavelength Modes Single, dual or multiple
Standard Curves Linear, quadratic, cubic, quartic, spline, polygon,

sigmoid or Akima
Additional Data Analysis Threshold, ratio, QC equations
Flexible Template Up to eight different well types.

Specifications
Power Requirements
Voltage Power Frequency
Main Unit 100 - 240 V 800 VA 50/60 Hz
Line Voltage Variation ± 10%
Line Frequency Variation ± 3 Hz
Environmental
Operating Range 15° C to 30° C
15% to 85% relative humidity (non-condensing)
2000 Meters Altitude
Computer Interface
Ports RS232 serial port
Baud Rate 19200. Character format.
Character Format 7 data bits, 1 stop bit, no parity
Standards
The instrument is designed in accordance with CSA 1010-1, CSA 1010-2-010,
UL 3101-1, EN 61010-1, EN 61010-2-010 and EN 61326-1.

Warning Labels
Warning Labels
The DSX™ Automated ELISA System or its components may contain
certain labels that that either warn the user of a hazard or note an electrical
connection. A hazard is something that can cause personal injury to the
operator or damage to the instrument. The labels that may be used on the
DSX™ Automated ELISA System are described below.
Label Description
Alternating current is present.
(English) Caution symbol. Refer to the

Routine Maintenance chapter.
(French) Attention (voir documents
d’accompagnement).
(English) Caution, motion hazard.

(French) Attention
(English) Caution, pinching or mechanical hazard.

(French) Attention
(English) Caution, hot surface.

(French) Attention, surface chaude.
Protective conductor terminal.
Earth (ground) terminal.
(English) Caution, risk of electric shock.

(French) Attention, risque de choc électrique.
Caution, biohazard.

Warning Labels

Unpacking
Chapter 3 Installation
! IMPORTANT: These installation procedures are

intended for trained personnel.
Unpacking
Materials Provided
Article Quantity
DSX™ Automated ELISA System 1
Instrument Power Cable 1
RS232 Communication Cable 1
Cleaning Wire 1
CD Containing Revelation™ Software Setup 1
Program and Electronic Operator’s Manual
Shipping Check List 1
Wash Buffer Containers 4

Liquid Waste Container 1
Reagent Rack 1
Tip Waste Container 1
Computer and Monitor (optional) 1
Consumables Sample Kit 1
Plastic Tweezers 1
Needle Nosed Pliers 1
3 mm Allen Wrench 1
4 mm Allen Wrench 1

Positioning the Instrument
To unpack the components:
1. Unpack the contents of the carton.
! CAUTION: The contents are heavy (approximately

110 Kg or 240 lbs) .
2. Place the DSX™ Automated ELISA instrument in the

approximate position where it will be located.
3. Examine the packaging to be sure that the power cord, the

computer connector cord and other materials have been
removed. Please save packaging material for future use.
4. Check to verify that all of the materials listed on the previous

page have been unpacked.
5. Inspect the components for damage. If damage is observed,

contact your shipper or service representative immediately.
Positioning the Instrument

Determine the area where the system will be located. You will need an area
that is approximately 106 cm (41.8 inches) wide, 91 cm (35.8 inches) deep,
and 80 cm (31.5 inches) high for the DSX™ Automated ELISA System.
The system should be positioned on a level surface that does not support
other devices that produce vibration (centrifuges, shaker bath, etc.). There
must be at least 20 cm (7.9 inches) of space at the rear of the instrument to
allow for sufficient ventilation.

Connecting the Computer System
Connecting the Computer System
! Note: A computer system is required but not

provided.
To connect the computer system:
1. Place the computer, keyboard, monitor and printer next to the

DSX System.
2. Plug the RS232 communication cable into an unused RS232

port on the computer. Note the computer port (i.e., COM1 or
COM2) that is used.
! Note: Refer to the instructions accompanying the

computer for the location of the ports and for
information on connecting components.
3. Plug the other end of the RS232 communication cable into the
bottom RS232 port at the right side of the instrument (Figure 19).
! Note: The lowest of the 3 RS232 ports must be

used. The other RS232 ports are used for diagnostic
purposes. See Figure 19.
4. Connect the keyboard, monitor and printer to the computer.
5. Connect the power to the computer, monitor and printer.
Cooling Power Power Cord

Fan Switch Connector
RS232 Ports
for Diagnostic
Purposes
RS232 Port
for
Connectio
Figure 19. View of the Right Side of the DSX™ Automated ELISA System

Connecting to a LIMS
The RS232C port is bi-directional when used in computer control mode.
The DSR/DTR handshake signals as well as software handshake are used
to maintain communication status and synchronization.
To Establish Computer Control Mode for the Reader
Computer Reader
1. DSR asserted
2. STX COMMAND ARGUMENTS

checksum ETX. Sends
command character string.
3. ACK/NAK Indicates
proper/improper reception
4. Executes task
5. ENQ Asks if computer is

ready to receive answer
6. ACK/NAK Indicates ready/not

ready to receive
7. STX RESULTS/ANSWER
checksum EXT Sends
answer
8. ACK/NAK Indicates
proper/improper reception
! Note: Steps 2 to 8 are repeated for subsequent

transmissions provided the data link remains
connected. Results may require more than a single
transmission, in which case Step 5 through Step 8
must be repeated. If the computer does not
acknowledge receipt of data (NAK) in either Step 6 or
in Step 8, the DSX will return to Step 5 and re-
transmit the results.

Checksum
The DSX Reader is equipped with a Fletcher’s checksum algorithm to
protect against any communication problems.
The checksum is calculated as follows:
sum 1 = 0
sum 2 = 0
for i from 1 to message length do
sum 1 = (sum 1 + message {i}) modulo 255
sum 2 = (sum 2 + sum 1) modulo 255
end for
checksum = sum 2*255 + sum 1
This checksum is transmitted as a 4 digit hexadecimal (base 16) number in

ASCII format.
Example:
Normal command STX 5 0 ETX
character ASCII code sum 1 sum 2

STX 2 0 0
‘5’ 53 55 57
‘0’ 48 103 160
Checksum = 160 x 255 + 103 = 40903

= 9FC716
The command therefore becomes: STX 5 0 9 F C 7 ETX

Loading Revelation™ Software

Revelation™ software is provided on a CD ROM. Before installing the
software on the personal computer, you will need the following information:
• The installation drive. Usually, this will be d.
• The installation directory.
To install Revelation Software:
1. Start Microsoft Windows NT.
2. Insert the installation CD ROM.
3. Select Start and then Run from the task bar.
4. Type d:\setup in the Run text box.
5. Click OK. The setup program will start and the Revelation™
Installation Window will be displayed.
! Note: To stop the installation, click on Exit.
6. The prompt Setup is complete is displayed when the installation

has been completed. Click on OK and remove the installation
CD ROM.
7. The Revelation™ program group is accessed from the Programs

section of the Start task bar.
! Note: The Revelation™ program icon can be added

to your Windows NT desktop using the Settings
section of the Start task bar.
! Or, a Revelation™ shortcut icon can be placed on

your computer desktop for convenient access to
Revelation™. See the following section for
instructions.

Creating a Shortcut Icon

A Revelation™ shortcut icon can be placed on your computer desktop in
two ways:
• (Recommended) A shortcut program is created during installation.
This program can be dragged to the desktop from Windows
Explorer.
• A new shortcut can be created.
to use the installed shortcut:
1. Open Microsoft Windows Explorer.
2. Locate the Revelation™ shortcut in the directory

c:\Windows\Start Menu\Programs\Revelation.
3. Drag the Revelation™ shortcut icon to the desktop.
! Note: Be sure to drag the Revelation™ shortcut, not

the Revelation™ Help shortcut.
4. Close Microsoft Windows Explorer.

Connecting the DSX Power Cord
Connecting the DSX Power Cord

The power cord connection to the DSX is made at the right side of the
system.
! Note: Depending upon local electrical codes and

electrical service quality, an optional uninterruptible
power supply (UPS) may be required in your
laboratory. The use of a UPS is optional but
recommended.
! CAUTION: The DSX System must be connected to

properly grounded electrical outlets. Obtain
assistance from a qualified electrician to verify that
your electrical outlets are properly grounded.
Before connecting the power cable, be sure that the

components have been connected to each other as
outlined in the previous section.
To connect the power cord:
1. Connect the power cord to the connector at the right side of the
instrument (Figure 19).
2. Connect the other end of the power cord to the laboratory

electrical supply outlet.

Starting the System
Starting the System

To start the system:
! CAUTION: Before starting the system, be sure that all

racks are properly seated and that the lids are
removed from all tubes, plates and sample tip racks.
1. Turn the DSX System power switch (Figure 19) ON.
2. Turn the power ON (if necessary) for the computer, monitor and
printer.
3. Double-click the Revelation™ shortcut icon. Or, select

Revelation from the Program menu in Windows NT.
4. A startup dialog box is displayed listing startup choices

(Figure 20).
5. Select Connect to DSX to operate the DSX System.
Or, select Configure Hardware to display the Setup DSX dialog

box for configuring the system (see page 48).
! Note: Select Connect in Demo Mode to run the

sofware in demonstration mode. The DSX System is
not connected during demonstration mode. Select
Quit Revelation to exit from the Startup dialog box.
Figure 20. Startup Dialog Box

Self Tests
Self Tests
A series of self-tests are carried out after Connect to DSX is selected from
the Startup dialog box. The test results are displayed upon completion of
the tests (Figure 21).
The self-test results should be printed on a monthly basis so that a record
can be kept of the performance of the DSX System. You can save test
results (the test results files are named *.tst) and then open them for review
or printout at a later date.
! NOTE: The self-test results should indicate whether

the system is operating properly. If a self-test failure
is reported in the Self-Test window, it is
recommended that you call Technical Service.
To print self-test results:
1. Select Print from the File menu while the self test results are
displayed.
! NOTE: If the DSX data files have been corrupted, the

DSX prompts for the serial number. This can be found
on the DSX rear panel.
Figure 21. Display of Self-Test Results

Configuring the Reader
Chapter 4 Preparing the System for Use

Configuring the reader allows the user to define the COM port, specify the
filters that are installed and specify conversion limits, specify maintenance
scripts, and specify a log file.
To configure the reader:
1. Select Configure Reader from the Tools menu. The Reader

Type dialog box is displayed (Figure 22).
2. Select Setup. The Setup DSX dialog box is displayed

(Figure 23).
! NOTE: The Setup DSX dialog box can also be

displayed by selecting Configure Hardware from the
Startup dialog box (Figure 20).
3. Specify the settings for each of the Reader configuration options.
! NOTE: Detailed instructions for an operation are

contained in the DSX Online Operator’s Manual,
accessed by selecting the Help button on the dialog
box that is being used.
Figure 22. Reader Type Dialog Box

Figure 23. Setup DSX Dialog Box

Editing Washer Plate Settings

The wash head on the DSX must be aligned so that the tips of the wash
pins are aligned with the base of the washer when the wash module is in
Standby.
To edit the washer plate settings:
1. Obtain a microplate of the type for which the washer is to be

aligned.
2. Eject the washer plate carrier and carefully place the microplate
in the carrier.
3. Retract the plate carrier.
4. Select Edit Washer Plates… from the Tools menu. The Edit
Washer Plate Settings dialog box is displayed (Figure 24).
5. Click New and enter a name for the type of plate for which the
wash settings are being entered.
Or: If settings for a plate that is already defined are being edited,
select the plate from the Plates Currently Defined panel.
6. Click the Show button opposite the Dispense Height field to

position the wash head in the dispense height position.
! Note: Refer to page 16 for a description of the various

wash height positions.
7. If the dispense height needs to be changed, enter the new value

in the Dispense Height field.
8. Click the Show button opposite the Dispense Height field to

position the wash head in the new dispense height position.
9. Repeat Steps 7 and 8 until the dispense height is correct.

Figure 24. Edit Washer Plate Settings Dialog Box
10. Repeat Steps 6 through 9 for each of the other wash height
positions.
11. Select the sweep mode and the sweep stroke.
! Important: Select No Sweep for sweep mode and

disable bottom washing whenever a C-bottom,
U-bottom or V-bottom plate is being used.
12. If sweep mode is enabled, specify the sweep stroke.
13. Click on Close to save the entered values.
! NOTE: Detailed instructions are contained in the DSX

Online Operator’s Manual, accessed by selecting the
Help button on the dialog box that is being used.

Selecting the Results Font
Selecting the Results Font

The user can select the font that is used when results reports are printed.
To select the results font:
1. Select Results Font from the Tools menu. The Font dialog box
is displayed (Figure 25).
2. Select the results font.

Figure 25. Font Dialog Box

Setting Options
Setting Options
Options allow the user to create assay short-cut buttons, set default plate
processing options (including auto save and auto print), select colors for
different well types, set default directories and enter the laboratory name,
address and phone number(s) to be printed on results reports.
To set options:
1. Select Options from the Tools menu. The Options dialog box is
displayed (Figure 26).
2. Specify the settings for each of the Options tabs.

Figure 26. Options Dialog Box

Changing the Password
Changing the Password

The system password can be changed.
To change the system password:
1. Select System Password from the Tools menu. The System

Password dialog box is displayed (Figure 27).
2. Specify a new system password.

Help button on the dialog box.
Figure 27. System Password Dialog Box

Specifying Consumables
Specifying Consumables
A database of all consumables and fluids is maintained in the software.
Once a consumable or fluid is defined in the database, it is available for
selection from a drop-down list during definition of an assay.
To enter information for a consumable:
1. Select the appropriate command (i.e., Edit Washer Fluids…)

from the Tools menu. The corresponding dialog box is
displayed.

Help button on the dialog box.
2. Reagent bottles, Sample tips and Reagent tips are all pre-
defined in the Revelation™ software. Refer to Reagents and
Pipetting in Chapter 1 Overview for the ordering information.

Filling the Wash Buffer Containers
Filling the Wash Buffer Containers

The Wash Buffer Containers must be filled with the appropriate wash buffer
and the dispense tubing and dispense pump power cable must be
connected to the front of the instrument.
To fill the Wash Buffer Containers:
1. Disconnect a Wash Buffer Container (see page 18) and remove

it from the system.
2. Remove the rear cover of the Wash Buffer Container

(Figure 12) and fill it with the wash solution that is to be used.
! Note: The Wash Buffer Containers each contain up to

two liters.
3. Make sure that the dispense tubing is routed through the

appropriate pinch valve at the front of the instrument.
4. Connect the dispense tubing to the Wash Buffer Container and
connect the Wash Buffer cable to the connector at the front of
the instrument (refer to Figure 12 for details).
! Note: Be sure that the wash tubing is not pinched or

crimped.
5. Repeat Steps 1 through 4 for the remaining wash buffer

containers.

Connecting the Waste Container
Connecting the Waste Container

The Waste Container must be connected to the front of the instrument.
To connect the Waste Container:
1. Route the waste tubing through the routing holes inside the front
panel of the instrument.
2. Connect the vacuum tubing and the waste tubing to the

connectors at the front of the instrument.
! Note: The connectors for the vacuum tubing and the

waste tubing are a different size so they cannot be
reversed.
! Note: Be sure that the waste tubing and the vacuum

tubing are not pinched or crimped.
3. Connect the level sensor cable to the connector at the front of

the instrument.

Creating a New Assay
Chapter 5 Defining an Assay
Creating a New Assay

The assay(s) for a worklist must be created before a worklist can be
prepared. Assay operations (pipetting, dispensing, washing and
incubating) and data reduction steps (see page 29) are specified in the
assay.
To create a new assay:
1. Select New then Assay from the File menu. A default assay is
displayed.
2. Define the assay operations and data reduction steps as

outlined below.
To define assay operations:
1. Display the default assay (see above).
2. Select the desired assay operation from the Operations menu.

The appropriate dialog box is displayed.
3. Define the operation.

To define data reduction steps:
1. Display the default assay (see above).
2. Select the desired data reduction step from the Data Reduction
menu. The appropriate dialog box is displayed.
3. Define the data reduction step.


Modifying an Assay
Modifying an Assay
An assay can be modified at any time.
To modify an assay:
1. Select Open from the File menu. The Open dialog box is
2. Select the assay to be modified.
3. Modify the assay operations and/or data reduction steps as

outlined on the previous page.
Figure 28. Open Dialog Box

Creating a New Worklist
Chapter 6 Creating a Worklist
Creating a New Worklist

A worklist specifies the assay(s) to be run on a series of samples. Once at
least one assay exists, a worklist can be created.
! NOTE: The worklist can include up to four

microplates.
Only one worklist can be open at a time.
More than one assay can be run on one plate if the

assays have the same incubation, washing and
shaking specifications.
To create a new worklist:
1. Select New then Worklist from the File menu. The Edit Worklist
dialog box (Figure 29) is displayed.
2. Define the worklist. Detailed instructions are contained in the

DSX Online Operator’s Manual, accessed by selecting Online
Manual from the Help menu.
Figure 29. Edit Worklist Dialog Box

Modifying a Worklist
Modifying a Worklist
An worklist can be modified at any time. However, only one worklist can be
open at a time.
To modify a worklist:
1. Select Edit from the File menu. The Open dialog box is
2. Select worklist (*wor.) as the file type.
3. Select the worklist to be modified.
4. Modify the worklist as outlined on the previous page.

Preparation
Chapter 7 Running a Worklist
Preparation
Prepare for a run by setting the runtime options, emptying the waste
container and filling wash buffer bottles with wash buffer (if required).
To set runtime options:
1. Display the worklist to be run (if needed)
2. Select Runtime Options from the Timeline menu. The Runtime

Options dialog box (Figure 30) is displayed.
3. Define the runtime options. Detailed instructions are contained in

the DSX Online Operator’s Manual, accessed by selecting
Online Manual from the Help menu.
Blue
White
Figure 30. Runtime Options Dialog Box

Preparation
To empty the Waste Container:
1. Disconnect the Waste Container (see page 20) and remove it

from the system.
2. Remove the cover of the Waste Container and discard the waste
in accordance with local regulations.
3. Rinse the waste container with DEIONIZED water. Discard the
rinse water.
! Note: If desired, the waste container can be

disinfected with a 10% (v/v) solution of household
bleach in water, or 70% ethanol. If bleach is used, the
container must be thoroughly rinsed with deionized
water before replacing, as residual bleach may affect
the results of ELISA assays.
4. Replace and tighten the waste cap.
! Note: Be sure that the waste cap is securely

tightened. Otherwise, a vacuum leak will cause the
software to create a vacuum error condition.
5. Place the Waste Container on the system and re-connect the

fittings.
To fill a wash buffer bottle:
1. Disconnect the Wash Buffer Container (see page 18) and

remove it from the system.
2. Remove the rear cover of the Wash Buffer Container
(Figure 12). Lay the cover on a clean paper towel.
3. Fill the wash buffer bottle with the wash buffer that is to be used.
! Note: If the wash buffer in a bottle is being changed,

discard the contents of the bottle and thoroughly
clean it before filling it with the new wash buffer.
4. Replace the cover and tighten it.

5. Place the Wash Buffer Container on the system and re-connect
the fittings.

Starting the Run
Starting the Run

To start the run:
1. Display the worklist to be run (if needed)
2. Select Start from the Timeline menu. A prompt to load the first
microplate is displayed (Figure 30).
3. Place the first microplate on the plate drawer in the position
shown.
4. Enter the plate ID (or read the plate barcode).
5. Click OK. The next prompt is displayed.
6. Follow additional instructions, making sure to load each item at
the designated position. After an item is loaded and information
(if any) is entered, click OK to display the next prompt.
! Important: All racks are mounted on dowel pins. Be

sure that each rack is securely seated and cannot
move in either the x- or y- direction.
7. Detailed instructions are contained in the DSX Online Operator’s
Manual, accessed by selecting Online Manual from the Help
menu.
Figure 31. Load Plate prompt

Monitoring Run Status
Monitoring Run Status
To monitor status of the run:
1. Display the worklist (if needed).
2. Select Worklist, Timeline, Consumables or Plate Layout from

the Timeline menu. The corresponding view of the run is
3. Detailed instructions are contained in the DSX Online Operator’s

Manual, accessed by selecting Online Manual from the Help
menu.
Figure 32. Timeline View

Routine Maintenance Procedures
Chapter 8 Service and Maintenance
The following periodic maintenance procedures are required for the

DSX™ Automated ELISA System:
Daily maintenance:
• Verify that the self-test passes.
! Note: Results of the self-test can be printed if desired.

Refer to page 46 for specifying printing of self-test
results.
• Empty and clean the Waste Tip Container.
! Warning: While the DSX alone does not present a

biohazard, the samples that are used and all parts
and consumables in contact with the samples must
be considered biohazardous.
• (As needed) Empty and clean the Liquid Waste Container.
! Note: If desired, the Waste Tip Container and the

Liquid Waste Container can be disinfected with a 10%
(v/v) solution of household bleach in water, or 70%
ethanol. If bleach is used, the containers must be
thoroughly rinsed with deionized water before
replacing, as residual bleach fumes may affect the
results of ELISA assays.
• Clean all plate drawers and external surfaces, using a towel
moistened with 70% alcohol.
• Purge the washer with 50mL of deionized water.
! Note: The deionized water used for purging should be

placed in Wash Buffer Container D (see Figure 11).

Weekly maintenance:
• Empty the Wash Buffer Containers and clean them with several
rinses of deionized water.
• Remove and clean the waste tip chute.
! Note: If desired, the waste tip chute can be

disinfected with 70% alcohol or by autoclaving it.
Bleach should not be used since it will corrode the
metal.
Six month maintenance:

• Replace the dispense tubing.
• Replace the aspiration tubing.
! Note: The dispense tubing and aspiration tubing may

need to be replaced more frequently than every six
months, depending upon the frequency of use and
the severity of operating conditions.
Contact DYNEX for information on replacement

tubing.

Cleaning and Decontamination
Cleaning and Decontamination
The DSX™ Automated ELISA System is constructed from materials that

resist chemical attack.
Spills should be cleaned as soon as possible. If you need to decontaminate

the DSX™ Automated ELISA System instrument (for example, before
servicing the instrument), clean the system and then decontaminate it as
described below.
! CAUTION: Always disconnect the power cable before

cleaning the instrument.
To clean the system:
1. Clean external surfaces with a cloth moistened with mild

laboratory detergent.
! Note: If needed, dilute the laboratory detergent

according to the manufacturer’s instructions before
using.
To decontaminate the system:
1. Wipe the surfaces with a cloth moistened with a 10% (v/v)

solution of household bleach in water or a 70% (v/v) solution of
alcohol.
! Note: Remove residual bleach from surfaces with a

cloth moistened with deionized water. Residual
bleach may affect the results of ELISA assays.

Removing a Module
Removing a Module
The incubator modules, absorbance module and the wash module can be
easily removed from the DSX™ Automated ELISA System.
To remove an incubator module or the absorbance module:
1. Loosen the fasteners (Figure 33) one-fourth (¼) of a turn using a

4 mm Allen wrench.
! Note: The incubator modules and the absorbance

module each have two fasteners.
2. Pull the module out of the housing.
3. Replace the module by reversing the procedure.
Fasteners
Figure 33. Fasteners on the Incubator Modules or Detection Module
To remove the wash module:
1. Remove the wash head by loosening the thumb screw.

2. Disconnect the tubing from the dispense valve on the washer
module.
3. Loosen the fasteners one-fourth (¼) of a turn using a 4 mm Allen
wrench.
! Note: The wash module has four fasteners.

4. Pull the module out of the housing.
4. Replace the module by reversing the procedure.

Replacing the Absorbance Module Lamp
! CAUTION: The optics assembly may be hot. Allow at

least five minutes for the instrument to cool before
opening the optics assembly.
Also, be careful when removing the filter access panel

as there is a possibility that the bulb may have
broken.
Failure to follow the lamp replacement procedures as

described may result in personal injury.
! CAUTION: Always disconnect the power before

removing the filter access panel.
To replace a lamp:
1. Remove the absorbance module from the system.

2. Remove the filter access panel to expose the optical assembly.
The bulb is mounted in the upper portion of the optical assembly
as shown in Figure 34.
Lamp
Figure 34. Optical Assembly

3. Put on a pair of rubber or latex gloves.
! CAUTION: Gloves are necessary to prevent skin oils

from damaging the lamp. Gloves are also worn as a
safety precaution should the glass bulb accidentally
break.
4. Grasp the lamp with plastic tweezers and pull the lamp out of its
receptacle (Figure 35).
! IMPORTANT: Use a gloved finger to prevent the bulb

from bumping into the metal sidewalls of the
enclosure during removal.
5. Obtain a replacement lamp.
6. Grasp the lamp tines with plastic tweezers and align the tines
with the corresponding holes in the lamp socket.
7. Firmly insert the lamp into the socket.
8. Replace the filter access panel.
9. Replace the absorbance module.
Figure 35. Removing the Lamp

Replacing an Absorbance Module Filter
! CAUTION: The optics assembly may be hot. Allow at

least five minutes for the instrument to cool before
opening the optics assembly.
Also, be careful when removing the filter access panel

as there is a possibility that the bulb may have
broken.

removing the filter access panel.
To remove a filter:
1. Remove the absorbance module from the system.
2. Remove the filter access panel. The filters are mounted on the
filter wheel as shown in Figure 34.
3. Locate the filter that is to be removed.
4. Firmly grasp the exterior filter housing with a pair of needle nose
pliers (Figure 36).
5. Pull the filter out of the spring loaded slot.
Figure 36. Removing a Filter

To install a filter:
1. Remove the filter access panel. The filters are mounted on the
filter wheel as shown in Figure 34.
2. Locate the filter position in which the filter will be installed.
! IMPORTANT: Filters must be installed in adjacent

filter positions, in the order of their wavelength. There
must not be any empty filter positions between the
lowest wavelength and highest wavelength filters.
! Note: If a new filter is installed in a previously empty

position or if the wavelength is being changed, the
wavelength of the new filter must be entered. See
page 47 for details.
3. Firmly grasp the exterior filter housing with a pair of needle nose
pliers.
4. Push the filter into the spring loaded slot.
! IMPORTANT: The bottom groove of the filter must be

firmly seated in the filter wheel. If the groove is
aligned with the spring on the filter wheel then the
filter has been installed incorrectly and will result in
invalid instrument performance.
6. Replace the absorbance module.

Replacing the Tubing
To replace the tubing:
1. Lift the rear edge of the metal panel at the front of the system
(Figure 37) to expose the tubing.
! Note: The metal panel is hinged at the front. When

lifting the front panel, prevent it from dropping all the
way forward.
Side
Panel
Figure 37. Lifting the Front Panel
2. Remove the four Allen screws from the left side panel, using a
3 mm Allen wrench, and remove the panel.
3. A diagram of the hydraulic tubing for the DSX™ Automated
ELISA System is shown in Figure 38. Consult the diagram for
routing of the tubing.
4. Replace the tubing and fittings as required.
5. Replace the side panel and secure it using the Allen screws.
6. Close the front panel.

Aspirate Wash
Tubing Head
Dispense
Tubing
To Vacuum Source
Vacuum
Vacuum
Sensor
Tubing
Figure 38. Hydraulic Tubing Diagram

Cleaning the Wash Head

servicing the system.
To remove the wash head assembly:
1. Grasp the wash head assembly and lift it up from the retaining
cradle.
2. Lift the wash head and tubing clear of the instrument. If

necessary, remove the wash tubing and waste tubing from the
retainer clips at the front of the instrument.
To clean the wash head assembly:
1. Pass the Cleaning Wire through the inside of each wash pin and
waste pin on the wash head.
2. Run a PURGE to rinse any material from the wash pins.
To replace the wash head assembly:
1. Position the wash head assembly back in the retaining cradle.
2. If necessary, replace the wash tubing and waste tubing into the
retainer clips at the front of the instrument.


Returning a Module for Service
Chapter 9 Service
If the instrument must be returned for service, it must be cleaned and

decontaminated if it has been in contact with potentially infectious body
fluids (including human blood), pathological samples, or toxic or radioactive
materials.
! Warning: While the DSX alone does not present a

biohazard, the samples that are used and all parts
and consumables in contact with the samples must
be considered biohazardous.
! Note: Refer to page 67 for cleaning and

decontamination instructions.
To return a module for service:
! Note: Refer to page 68 for module removal

instructions.
1. Contact the nearest DYNEX technical service facility for return

authorization.
2. Clean and decontaminate the module.
3. Fill out an Equipment in Transit form (Figure 39).
4. Pack the module and the Equipment in Transit form for

shipment.
5. After you receive a return authorization, ship the module to the

nearest DYNEX facility (see page 78).

Figure 39. Equipment in Transit Form

Limited Warranty
Limited Warranty
Warranty and Special Provisions
DYNEX TECHNOLOGIES, INC. products are fully guaranteed for one year against
defects in parts, materials, and workmanship. Defective parts and materials will be
replaced or, at the discretion of DYNEX, repaired at no charge for a period of one
year and labor required for such replacement or repair will be provided at no charge
for a period of one year, provided that the products are utilized and maintained in
accordance with the instructions in the applicable operating and servicing manual,
and provided further that the products have not, as determined solely by DYNEX,
been subject to misuse or abuse by the Customer or other parties unrelated to
DYNEX. DYNEX makes no warranty, expressed or implied, as to the fitness of any
product for any particular purposes other than those purposes described in the
applicable operating and servicing manual, nor does DYNEX make any other
warranty, whether expressed or implied, including merchantability, other than those
appearing on the face hereof. Where DYNEX guarantees any product, whether
under this Warranty or as a matter of law, and there is a breach of such guarantee,
the Customer's only and exclusive remedy shall be the replacement or repair of
defective parts and materials, as described above. This shall be the limit of
DYNEXs liability. Furthermore, DYNEX shall not be liable for incidental or
consequential damages. Failure of the Customer to notify DYNEX of a claimed
defect by registered mail within thirty days of the discovery thereof shall constitute a
waiver of any claim for breach of warranty.
When a product is required by DYNEX to be installed by a DYNEX engineer or
technician, the period of this Warranty shall begin on the date of such installation,
provided, however, that any use of the product prior to such installation shall, at the
sole election of DYNEX. void this Warranty. When installation by DYNEX personnel
is not required, the period of this Warranty shall begin on the date of shipment from
DYNEX. The period of this Warranty shall begin as described above whether or not
the product has been installed or shipped pursuant to a purchase order, and any trail
period shall be deducted from the Warranty period that would otherwise apply under
a subsequent placed purchase order for that product.
Limitation of Liability. Notwithstanding anything to the contrary contained herein, the
liability of SELLER (whether by reason of breach of warranty, breach of contract,
tort, or otherwise), including without limitation under any indemnification provision
contained herein, shall be limited to replacement of goods returned to DYNEX which
are shown to DYNEX ’s reasonable satisfaction to have been nonconforming or to
refund the purchase price, or, if not paid, to a credit amount of the purchase price
therefor.
THE FOREGOING WARRANTIES ARE EXCLUSIVE AND ARE GIVEN
AND ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES,
EXPRESS OR IMPLIED, INCLUDING WITHOUT LIMITATION, THE
IMPLIED WARRANTY OF MECHANTABILITY AND THE IMPLIED
WARRANTY OF FITNESS FOR A PARTICULAR PURPOSE. NEITHER
PARTY SHALL BE LIABLE TO THE OTHER FOR ANY INCIDENTAL,
INDIRECT, SPECIAL, OR CONSEQUENTIAL DAMAGES.

Sales Offices
Sales Offices
Please contact your nearest sales office for accessories, technical

assistance or information on product servicing.
Dynex Technologies, Inc. Dynex Hybaid Labsystems

Unit 20E, 20th Floor, Centre 600 Berner Strasse 53
82 King Lam Street 60437 Frankfurt am Main
Cheung Sha Wan, Kowloon Germany
Hong Kong Tel: +49 (0) 69/50919680
China Fax: +49 (0) 69/50919698
Tel: +852 25743389 E-mail: labsystems.de@thermobio.com
Fax: +852 28345217
E-mail: aua@dynextechnologies.com
Dynex Hybaid Labsystems Dynex Labsystems

Na Cihadle, 32 Action Court
160 00 Praha 6 Ashford Road
Czech Republic Middlesex TW15 1XB
Tel: +420 2 24 31 33 75 U.K.
Fax: +420 2 24 32 01 33 Tel: +44 (0) 1784 251 225
E-mail: office@dynex.cz Fax: +44 (0) 1784 258 739
E-mail: o.sales@dynex.co.uk
Labsystems Oy Dynex Technologies, Inc.

Sorvaajankatu 15 14340 Sullyfield Circle
00810 Helsinki Chantilly, VA 20151
Finland USA
Tel: 358-9-329-100 Tel: +1 703-631-7800
Fax: 358-9-3291-0312 Toll Free: 800-336-4543
Fax: +1 703-631-7816
E-mail: info@dynextechnologies.com
Dynex Hybaid Labsystems
Eragny Parc - BP 249
95615 Cergy Pontoise Cedex
France
Tel: +33 1 34 32 51 51
Fax: +33 1 34 32 51 59
E-mail:
labsystems.france@thermobio.com

Index
Index
absorbance, 24, 25 Operations menu, 29
accessories parity, 34
dilution plate, 8 password, 53
liquid waste container, 20 pipette module, 11
standard/control rack, 1 pipetting
wash buffer containers, 18 profiles, 12
ambient drawer, 9 plate drawer, 18
ASPIRATE, 17 positioning, 38
aspiration height, 16 power ON, 44
assay definition, 29 Purge, 18
blanking, 25 PURGE, 17
checksum, 41 return of a module, 77
cleaning, 67 RS232, 34, 39
configuration options, 27 self-tests, 46
consumables, 54 SOAK, 17
sample tips, 2 stop bits, 34
data bits, 34 sweep height, 16
decontamination, 67 test filter, 24
dilution tubing, 73
protocols, 13 wash, 55
Dimensions, 33 unpacking, 38
DISPENSE, 17 Unpacking, 37
dispense height, 16 UPS, 44
DSX usage area, 38
description, 5 warning labels, 35
illustration, 5 Wash Container
DYNEX facilities, 80 cleaning, 55, 62
FILL, 17 installing, 55
Filters refilling, 55, 62
Dual Mode, 24 wash head
installing, 71 cleaning, 75
Multiple Mode, 25, 33 positions, 16
reference filter, 25 replacement, 75
Single Mode, 33 wash pins, 15
test filter, 24 wash module, 15
footprint, 38 wash pins, 15
handshake, 40 wash protocol
lamp, 23, 68, 69, 70 operations, 17
maintenance waste, 20
daily, 65 Waste Container
six-month, 65 cleaning, 62
weekly, 65 emptying, 62
microplates wavelength, 24, 25, 33
deep well, 13 well top, 16
MOVE, 17 worklist, 31, 59, 60, 61

Index

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DSX™ Automated ELISA System

 2000 This document is the copyright of DYNEX TECHNOLOGIES and

Table of Contents ......................................................................................................... i

About this Manual........................................................................................................iii

DSX™ System Operator’s Manual i

Connecting the DSX Power Cord ...................................................................... 44

ii DSX™ System Operator’s Manual

About this Manual

DSX™ System Operator’s Manual iii

This page is intentionally left blank

iv DSX™ System Operator’s Manual

Figure 1. The DSX™ Automated ELISA System

DSX™ System Operator’s Manual 1

2 DSX™ System Operator’s Manual

• Different assays can be run on the same plate

DSX™ System Operator’s Manual 3

This page is intentionally left blank

4 DSX™ System Operator’s Manual

! CAUTION: Power is on to the system and to the

Wash Buffer/ Pipette Work System Absorbance Wash Incubator

Figure 2. Location of Principal Hardware Components

DSX™ System Operator’s Manual 5

! CAUTION: The electrical interlock for the system

! CAUTION: Pinching hazard. Be sure that your hands

Handle System Cover

Figure 3. System Cover

6 DSX™ System Operator’s Manual

Sample Dilution Reagent Reagent Tip Waste

Figure 4. The Workspace

DSX™ System Operator’s Manual 7

Reagent bottles are contained on the reagent rack, which contains up to

8 DSX™ System Operator’s Manual

! The microplate positions are numbered from 1 to 4.

Position 1 Position 2 Position 3 Position 4

Figure 5. The Ambient Drawer

DSX™ System Operator’s Manual 9

! Whenever this manual refers to a microplate, it is

Figure 6. Plate Holder

10 DSX™ System Operator’s Manual

Microplate The microplate handler transfers a microplate

Figure 7. The Pipette Module

DSX™ System Operator’s Manual 11

Separate pipetting profiles ranging from 1 to 5 can be specified for any

! CAUTION: Changing the pipette profile can affect

! The default pipetting profile is 4. The pipetting profile

! The pipetting signature is specified during definition of

Sample Mixing During Dilution

12 DSX™ System Operator’s Manual

The dilution modes are described below:

Dilution Mode Description

Microplate Ratios of sample to diluent range from 11:1 to

DSX™ System Operator’s Manual 13

! Note: Refer to page 29 for a summary of the

! Note: The incubator modules are labelled as 1, 2, 3

Figure 8. Incubator Modules

14 DSX™ System Operator’s Manual

! Note: Refer to page 29 for a summary of the wash