Module 2 - The Metaplasmic Materials in Cells
Module 2 - The Metaplasmic Materials in Cells
Module 2 - The Metaplasmic Materials in Cells
LEARNING OBJECTIVES
At the end of this module, you should be able to appreciate the presence of metaplasmic materials in the cells.
ENGAGE
Metaplasmic materials or ergastic bodies are residual elements of the cytoplasm that are metabolic products of
the cell. Examples of these metabolic products are pigment granules, oil, fat, starch and others. These metaplasms
could be for storage such as glycogen or fat.
EXPLORE
Note the changes in color in the different specimens subjected to different reagents to show the metaplasmic
materials present. These result images were previously taken in the laboratory.
HPO
400X
Banana Benedict’s solution
Peanut Sudan III
HPO
400X
Bean cotyledon Nitric acid and Ammonia
HPO
400X
Beef fat Glycerin
HPO
400X
EXPLAIN
Cell inclusions are considered various nutrients or pigments that can be found within the cell, but do not have
activity like other organelles. Examples of cell inclusions are glycogen, lipids, and pigments such as melanin,
lipofuscin, and hemosiderin.
Lipid spheres in tissues are caused by an accumulation of triglycerides and appear as perfectly spherical
structures. Due to processing, lipids cannot be visualized in paraffin-embedded tissues; it is assumed lipid was
present when clear, spherical structures are present. On frozen tissue, lipids can be visualized using stains such as
Sudan. The material is prominent in adipocytes (fat cells), and may be found in any cell.
Numerous pigments can be observed in tissues and cells. Melanin is a brown pigment in hair and skin. These are
dense ellipsoidal granules produced by specialized cells called melanocytes and are transferred to hair and skin
cells to produce a pigmentary change.
A second pigment often found in cells and tissues is lipofuscin. Lipofuscin often appears as a brown-yellow color
that can be autofluorescent and accumulates over time, giving it the name “age pigment”. It is mainly found in the
lysosomes of post mitotic cells. Accumulation of lipofuscin can have detrimental effects on the cell by disturbing
cellular processes and leading to a degradation of cellular activity.
In plants, metaplasmic materials could be present in the cell walls or vacuoles or in the organelles of
protoplasm. These substances belong to 4 categories: reserve food, inorganic materials, secretory products and
excretory products. Starch grains are found in all parts of the plant although in storage organs.
ELABORATE
Potato
Banana
Peanut
Bean cotyledon
Beef fat
Note: This assessment is graded. Accomplish properly and submit them on/before the specified deadline.
SUBMISSION OF OUTPUT:
For OBL: Post your file on our google classroom submission bin. Rename the file as LAST NAME_Given
Name_ Elaborate e.g. DELA CRUZ_JOHN_ELABORATE
For CBL: Save in OTG and send via courier or e-mail to instructor. Rename the file as LAST NAME_Given
Name_ Elaborate e.g. DELA CRUZ_JOHN_ELABORATE
EVALUATE
Instructions: Explain the changes in color in the different specimens subjected to different reagents. Include in-
text citations and references (follow APA 6th v). Be guided by the rubric found in Appendix A.
Specimen Explanation
Potato
Banana
Peanut
Bean cotyledon
Beef fat
Note: This assessment is graded. Accomplish properly and submit them on/before the specified deadline.
SUBMISSION OF OUTPUT:
For OBL: Post your file on our google classroom submission bin. Rename the file as LAST NAME_Given
Name_ Evaluate e.g. DELA CRUZ_JOHN_EVALUATE
For CBL: Save in OTG and send via courier or e-mail to instructor. Rename the file as LAST NAME_Given
Name_ Evaluate e.g. DELA CRUZ_JOHN_EVALUATE
REFERENCES
Principles of Anatomy and Physiology (2014) by Gerard J. Tortora and Bryan N N. Derrickson
N e x t