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    Observation of Onion Cell-1

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    shamim nazam
    The document provides instructions for observing onion cells under a light microscope. The procedure involves placing an onion membrane on a slide, staining it with iodine or methylene blue, covering it, and viewing it under low power on a microscope. Observations of onion cells show large, rectangular cells with distinct cell walls, dark stained nuclei, large central vacuoles, and small cytoplasmic granules. Plant cells have a more regular shape than animal cells due to their rigid cellulose cell wall.

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    Observation of Onion Cell-1

    Uploaded by

    shamim nazam
    132 views1 page
    The document provides instructions for observing onion cells under a light microscope. The procedure involves placing an onion membrane on a slide, staining it with iodine or methylene blue, covering it, and viewing it under low power on a microscope. Observations of onion cells show large, rectangular cells with distinct cell walls, dark stained nuclei, large central vacuoles, and small cytoplasmic granules. Plant cells have a more regular shape than animal cells due to their rigid cellulose cell wall.

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    The document provides instructions for observing onion cells under a light microscope. The procedure involves placing an onion membrane on a slide, staining it with iodine or methylene blue, covering it, and viewing it under low power on a microscope. Observations of onion cells show large, rectangular cells with distinct cell walls, dark stained nuclei, large central vacuoles, and small cytoplasmic granules. Plant cells have a more regular shape than animal cells due to their rigid cellulose cell wall.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    132 views1 page

    Observation of Onion Cell-1

    Uploaded by

    shamim nazam
    The document provides instructions for observing onion cells under a light microscope. The procedure involves placing an onion membrane on a slide, staining it with iodine or methylene blue, covering it, and viewing it under low power on a microscope. Observations of onion cells show large, rectangular cells with distinct cell walls, dark stained nuclei, large central vacuoles, and small cytoplasmic granules. Plant cells have a more regular shape than animal cells due to their rigid cellulose cell wall.

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    © All Rights Reserved
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    PRACTICAL: OBSERVATION OF ONION CELL BY LIGHT MICROSCOPY

    Procedure:

    1. Add a drop of water at the center of the microscopic slide


    2. Having pulled of a thin membrane from the onion layer, lay it at the center of the
    microscopic slide (the drop of water will help flatten the membrane)
    3. Add a drop of iodine solution on the onion membrane (or methylene blue)
    4. Gently lay a microscopic cover slip on the membrane and press it down gently using a
    needle to remove air bubbles.
    5. Touch a blotting paper on one side of the slide to drain excess iodine/water solution,
    6. Place the slide on the microscope stage under low power to observe.
    7. Adjust focus for clarity to observe.

    Observations

     Large, rectangular interlocking cells,


     Clearly visible distinct cell walls surrounding the cells,
     Dark stained nucleus,
     Large vacuoles at the center,
     Small granules may be observed inside the cells (within the cytoplasm)

    Unlike animal cells,plant cells have a more regular shape. This is because they have a cell wall
    made up of cellulose which maintains its shape.

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