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Group 3 Postlab Lipids

Thin-layer chromatography and column chromatography are compared. Thin-layer chromatography uses a glass plate as the stationary phase, while column chromatography uses purified siliceous earth beads. In thin-layer chromatography, the mobile phase migrates up the plate via capillary action, while in column chromatography the mobile phase flows through the stationary phase. Ninhydrin spray and iodine vapor are used to visualize lipids separated by thin-layer chromatography, with ninhydrin detecting amino groups and iodine detecting unsaturated lipids.

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98 views2 pages

Group 3 Postlab Lipids

Thin-layer chromatography and column chromatography are compared. Thin-layer chromatography uses a glass plate as the stationary phase, while column chromatography uses purified siliceous earth beads. In thin-layer chromatography, the mobile phase migrates up the plate via capillary action, while in column chromatography the mobile phase flows through the stationary phase. Ninhydrin spray and iodine vapor are used to visualize lipids separated by thin-layer chromatography, with ninhydrin detecting amino groups and iodine detecting unsaturated lipids.

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© © All Rights Reserved
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Post- Lab

Analysis of Lipids in Egg Yolk


Name: Group No. : 3
Section: 2E-PH Date Submitted:10/19/20

1. Differentiate thin-layer chromatography from column chromatography based on


(1) the separation of their components in the stationary and mobile phases, (2)
solvent migration, and (3) type of support used.

Thin-layer Chromatography Column Chromatography

m
er as
Separation of The stationary phase or silica The stationary phase or silica gel

co
Components in gel is spotted in the baseline beads are placed in a cylindrical

eH w
the stationary of the TLC plate while the tube or “column” while the

o.
and mobile mobile phase or the solvent is mobile phase or the solvent is
phases rs e
placed in the developing added or poured on the top.
ou urc
chamber.

Solvent When the TLC plate is put In Column Chromatography,


o

Migration inside the developing chamber different components travel at


aC s

where the solvent is located, different rates. The solvent or the


vi y re

the mobile phase or the mobile phase which was poured


solvent will migrate up to the on top of the silica gel flows
TLC plate by capillary action. through the stationary phase. Its
ed d

migration is faster than the


ar stu

stationary phase therefore it will


be eluted first. Since the
stationary phase is slower it will
is

then eluate later.


Th

Type of support The type of support commonly The type of support commonly
used used in Thin-layer used in Column Chromatography
Chromatography is a glass is called purified siliceous earth or
sh

plate. a silica which was purified


through calcining.

This study source was downloaded by 100000830329275 from CourseHero.com on 08-21-2021 03:48:32 GMT -05:00

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2. What is the purpose of using ninhydrin and iodine in thin-layer chromatography.

Ninhydrin spray and iodine vapor is used as a visualizing agent in thin-layer


chromatography. Iodine vapor detects unsaturated lipids and will result in yellow-
brown spots. On the other hand, Ninhydrin spray detects free amino groups, and
will result in purple spots.

m
er as
co
eH w
o.
rs e
ou urc
o
aC s
vi y re
ed d
ar stu
is
Th
sh

This study source was downloaded by 100000830329275 from CourseHero.com on 08-21-2021 03:48:32 GMT -05:00

https://www.coursehero.com/file/72753346/Group-3-Postlab-Lipids/
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